CENPA cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPA
Entrez Gene ID	100113359
Full Name	centromere protein A
Synonyms	CENP-A,ggCENP-A
General protein information	Preferred Names centromere protein A Names histone H3-like centromeric protein A
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101037.2	NP_001094507.1	histone H3-like centromeric protein A
XM_015294936.1	XP_015150422.1	histone H3-like centromeric protein A isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CENPA	XP_515346.2
	CENPA	XP_001087306.1
Gallus gallus (chicken)	CENPA	NP_001094507.1
Xenopus tropicalis (tropical clawed frog)	cenpa	NP_001016585.1
Canis lupus familiaris (dog)	CENPA	XP_532899.2
Mus musculus (house mouse)	Cenpa	NP_031707.1
Bos taurus (cattle)	LOC782601	XP_005225982.1
Homo sapiens (human)	CENPA	NP_001800.1
Bos taurus (cattle)	CENPA	NP_001192309.1
Rattus norvegicus (Norway rat)	Cenpa	NP_001100181.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of M18BP1/KNL2 with CENP-A Nucleosome Is Essential for Centromere Formation in Non-mammalian Vertebrates.
Hori T, Shang WH, Hara M, Ariyoshi M, Arimura Y, Fujita R, Kurumizaka H, Fukagawa T
Developmental cell42(2)181-189.e3(2017 07)

The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly.
Hori T, Shang WH, Takeuchi K, Fukagawa T
The Journal of cell biology200(1)45-60(2013 Jan)

CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1.
Okada M, Okawa K, Isobe T, Fukagawa T
Molecular biology of the cell20(18)3986-95(2009 Sep)

CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore.
Hori T, Amano M, Suzuki A, Backer CB, Welburn JP, Dong Y, McEwen BF, Shang WH, Suzuki E, Okawa K, Cheeseman IM, Fukagawa T
Cell135(6)1039-52(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

M18BP1/KNL2 is essential for centromere formation through direct binding to CENP-A nucleosome in non-mammalian vertebrates.
Title: Association of M18BP1/KNL2 with CENP-A Nucleosome Is Essential for Centromere Formation in Non-mammalian Vertebrates.

CCAN has two distinct roles: recruiting CENP-A to establish the kinetochore and serving as a structural core to directly recruit kinetochore proteins.
Title: The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly.

CENP-H-containing complex facilitates deposition of newly synthesized CENP-A into centromeric chromatin in cooperation with FACT and CHD1.
Title: CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1.

Data show that CENP-A induces some positioning of its own and some unwrapping at the entry-exit relative to canonical nucleosomes on both 5 S DNA and the alpha-satellite sequence on which it is normally loaded.
Title: CENP-A-containing nucleosomes: easier disassembly versus exclusive centromeric localization.

CENP-A-depleted cells exhibit a specific defect in maintaining kinetochore localization of the checkpoint protein BubR1 under conditions of checkpoint activation
Title: CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1.

The following CENPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa51394	NM_001101037.2 Latest version!	Gallus gallus centromere protein A (CENPA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00
OGa43136	XM_015294936.1 Latest version!	Gallus gallus centromere protein A (CENPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPA ( NM_001101037.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001094507.1 Gallus gallus centromere protein A (CENPA), mRNA.

Price & Availability↑

CloneID	OGa51394
Clone ID Related Accession (Same CDS sequence)	NM_001101037.2
Accession Version	NM_001101037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-15
Organism	Gallus gallus(chicken)
Product	histone H3-like centromeric protein A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05001139.1 and AH012838.2. On Aug 16, 2016 this sequence version replaced NM_001101037.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201358, SAMEA2201364 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCGGC CCAAACCGCG CAGCCCCCGG CGCCGGGGCC GCCCCCCCCC GGCCGCCCCC 
CCGCCACCCC CCGCGCGGCC TCGCGCCCGC CGGTACCGGC CCGGTCAGAG GGCGCTGCGG 
GAGATCCGCC GCTATCAGAG CAGCACCGCT CTGCTGCTGC GCCGCCAGCC CTTCGCGCGC 
GTGGTGCGGG AGATCTGCTT GCTCTTCACA CGGGGCGTGG ACTACAGGTG GCAGGCTATG 
GCCCTATTGG CCCTACAGGA GGCGGCGGAG GCATTCCTGG TGCACCTGCT GGAGGACGCC 
TACCTGTGCT CGCTGCATGC GCGCCGCGTC ACACTGTACC CCAAAGACCT GCAGCTGGCA 
CGGCGCCTGC GGGGGCTGCA GGGGGAGGGG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094507.1
CDS	1..396
Translation	MPRPKPRSPRRRGRPPPAAPPPPPARPRARRYRPGQRALREIRRYQSSTALLLRRQPFARVVREICLLFTRGVDYRWQAMALLALQEAAEAFLVHLLEDAYLCSLHARRVTLYPKDLQLARRLRGLQGEGF

Target ORF information:

RefSeq Version	NM_001101037.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centromere protein A (CENPA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101037.2

ORF Insert Sequence:

ATGCCGCGGC CCAAACCGCG CAGCCCCCGG CGCCGGGGCC GCCCCCCCCC GGCCGCCCCC 
CCGCCACCCC CCGCGCGGCC TCGCGCCCGC CGGTACCGGC CCGGTCAGAG GGCGCTGCGG 
GAGATCCGCC GCTATCAGAG CAGCACCGCT CTGCTGCTGC GCCGCCAGCC CTTCGCGCGC 
GTGGTGCGGG AGATCTGCTT GCTCTTCACA CGGGGCGTGG ACTACAGGTG GCAGGCTATG 
GCCCTATTGG CCCTACAGGA GGCGGCGGAG GCATTCCTGG TGCACCTGCT GGAGGACGCC 
TACCTGTGCT CGCTGCATGC GCGCCGCGTC ACACTGTACC CCAAAGACCT GCAGCTGGCA 
CGGCGCCTGC GGGGGCTGCA GGGGGAGGGG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPA ( XM_015294936.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150422.1 Gallus gallus centromere protein A (CENPA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa43136
Clone ID Related Accession (Same CDS sequence)	XM_015294936.1
Accession Version	XM_015294936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	histone H3-like centromeric protein A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455973.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGGC CCAAACCGCG CAGCCCCCGG CGCCGGGGCC GCCCCCCCCC GGCCGCCCCC 
CCGCCACCCC CGCGCGCCCG CCGGTACCGG CCCGGTCAGA GGGCGCTGCG GGAGATCCGC 
CGCTATCAGA GCAGCACCGC TCTGCTGCTG CGCCGCCAGC CCTTCGCGCG CGTGGTGCGG 
GAGATCTGCT TGCTCTTCAC ACGGGGCGTG GACTACAGGT GGCAGGCTAT GGCCCTATTG 
GCCCTACAGG AGGCGGCGGA GGCATTCCTG GTGCACCTGC TGGAGGACGC CTACCTGTGC 
TCGCTGCATG CGCGCCGCGT CACACTGTAC CCCAAAGACC TGCAGCTGGC ACGGCGCCTG 
CGGGGGCTGC AGGGGGAGGG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150422.1
CDS	260..646
Translation	MPRPKPRSPRRRGRPPPAAPPPPPRARRYRPGQRALREIRRYQSSTALLLRRQPFARVVREICLLFTRGVDYRWQAMALLALQEAAEAFLVHLLEDAYLCSLHARRVTLYPKDLQLARRLRGLQGEGF

Target ORF information:

RefSeq Version	XM_015294936.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus centromere protein A (CENPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294936.1

ORF Insert Sequence:

ATGCCGCGGC CCAAACCGCG CAGCCCCCGG CGCCGGGGCC GCCCCCCCCC GGCCGCCCCC 
CCGCCACCCC CGCGCGCCCG CCGGTACCGG CCCGGTCAGA GGGCGCTGCG GGAGATCCGC 
CGCTATCAGA GCAGCACCGC TCTGCTGCTG CGCCGCCAGC CCTTCGCGCG CGTGGTGCGG 
GAGATCTGCT TGCTCTTCAC ACGGGGCGTG GACTACAGGT GGCAGGCTAT GGCCCTATTG 
GCCCTACAGG AGGCGGCGGA GGCATTCCTG GTGCACCTGC TGGAGGACGC CTACCTGTGC 
TCGCTGCATG CGCGCCGCGT CACACTGTAC CCCAAAGACC TGCAGCTGGC ACGGCGCCTG 
CGGGGGCTGC AGGGGGAGGG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of M18BP1/KNL2 with CENP-A Nucleosome Is Essential for Centromere Formation in Non-mammalian Vertebrates.
Developmental cell42(2)181-189.e3(2017 07)
Hori T,Shang WH,Hara M,Ariyoshi M,Arimura Y,Fujita R,Kurumizaka H,Fukagawa T

The CCAN recruits CENP-A to the centromere and forms the structural core for kinetochore assembly.
The Journal of cell biology200(1)45-60(2013 Jan)
Hori T,Shang WH,Takeuchi K,Fukagawa T

CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1.
Molecular biology of the cell20(18)3986-95(2009 Sep)
Okada M,Okawa K,Isobe T,Fukagawa T

CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore.
Cell135(6)1039-52(2008 Dec)
Hori T,Amano M,Suzuki A,Backer CB,Welburn JP,Dong Y,McEwen BF,Shang WH,Suzuki E,Okawa K,Cheeseman IM,Fukagawa T

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CENPA cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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