gRNAs
The following CFTR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFTR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa29014 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015275972.1 , XM_015275972.2 | |
| Accession Version | XM_015275972.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | cystic fibrosis transmembrane conductance regulator isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455712.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCAGAGGT CCCCCCTGGA GAAGGCGAAC ATCTTCTCCA AACTTTTCTT CAGATGGACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015131458.1 |
| CDS | 360..4817 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_015275972.1 |
1 | ATGCAGAGGT CCCCCCTGGA GAAGGCGAAC ATCTTCTCCA AACTTTTCTT CAGATGGACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa29014 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015275972.1 , XM_015275972.2 | |
| Accession Version | XM_015275972.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | cystic fibrosis transmembrane conductance regulator isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015275972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCAGAGGT CCCCCCTGGA GAAGGCGAAC ATCTTCTCCA AACTTTTCTT CAGATGGACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015131458.1 |
| CDS | 370..4827 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015275972.2 |
1 | ATGCAGAGGT CCCCCCTGGA GAAGGCGAAC ATCTTCTCCA AACTTTTCTT CAGATGGACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa25560 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001105666.1 | |
| Accession Version | NM_001105666.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-07-15 | |
| Organism | Gallus gallus(chicken) | |
| Product | cystic fibrosis transmembrane conductance regulator | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC159212.3 and AC138565.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. | |
1 | ATGCAGAGGT CCCCCCTGGA GAAGGCGAAC ATCTTCTCCA AACTTTTCTT CAGATGGACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001099136.1 |
| CDS | 4..4458 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001105666.1 |
1 | ATGCAGAGGT CCCCCCTGGA GAAGGCGAAC ATCTTCTCCA AACTTTTCTT CAGATGGACA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of Cl |
CFTR mediated chloride secretion in the avian renal proximal tubule. |
Comparative analyses of multi-species sequences from targeted genomic regions. |
