DAG1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAG1
Entrez Gene ID	100049058
Full Name	dystroglycan 1
General protein information	Preferred Names dystroglycan 1 Names dystroglycan dystroglycan 1 (dystrophin-associated glycoprotein 1)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001097540.1	NP_001091009.1	dystroglycan precursor
XM_025154393.1	XP_025010161.1	dystroglycan isoform X1
XM_025154391.1	XP_025010159.1	dystroglycan isoform X1
XM_025154392.1	XP_025010160.1	dystroglycan isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DAG1	NP_776587.1
Danio rerio (zebrafish)	dag1	NP_775381.1
Canis lupus familiaris (dog)	DAG1	NP_001029164.1
Homo sapiens (human)	DAG1	NP_001171105.1
Mus musculus (house mouse)	Dag1	NP_001263421.1
Rattus norvegicus (Norway rat)	Dag1	NP_446149.1
Pan troglodytes (chimpanzee)	DAG1	XP_003339091.1
	DAG1	XP_001108436.1
Gallus gallus (chicken)	DAG1	NP_001091009.1
Xenopus tropicalis (tropical clawed frog)	dag1	NP_001016518.1

Gene Ontology
Bibliography

Related articles in PubMed

Dystroglycan regulates structure, proliferation and differentiation of neuroepithelial cells in the developing vertebrate CNS.
Schr?der JE, Tegeler MR, Grosshans U, Porten E, Blank M, Lee J, Esapa C, Blake DJ, Kr?ger S
Developmental biology307(1)62-78(2007 Jul)

Aberrant glycosylation of alpha-dystroglycan causes defective binding of laminin in the muscle of chicken muscular dystrophy.
Saito F, Blank M, Schr?der J, Manya H, Shimizu T, Campbell KP, Endo T, Mizutani M, Kr?ger S, Matsumura K
FEBS letters579(11)2359-63(2005 Apr)

The effects of post-translational processing on dystroglycan synthesis and trafficking.
Esapa CT, Bentham GR, Schr?der JE, Kr?ger S, Blake DJ
FEBS letters555(2)209-16(2003 Dec)

Agrin is a high-affinity binding protein of dystroglycan in non-muscle tissue.
Gesemann M, Brancaccio A, Schumacher B, Ruegg MA
The Journal of biological chemistry273(1)600-5(1998 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dystroglycan plays a key role in maintaining neuroepithelial cell morphology, and that interference with dystroglycan function influences proliferation and differentiation of neuroepithelial cells
Title: Dystroglycan regulates structure, proliferation and differentiation of neuroepithelial cells in the developing vertebrate CNS.

aberrant glycosylation of alpha-DG in the pathogenesis of muscular degeneration in this model animal of muscular dystrophy
Title: Aberrant glycosylation of alpha-dystroglycan causes defective binding of laminin in the muscle of chicken muscular dystrophy.

The following DAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa143863	XM_025154393.1 Latest version!	Gallus gallus dystroglycan 1 (DAG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa143863	XM_025154391.1 Latest version!	Gallus gallus dystroglycan 1 (DAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa143863	XM_025154392.1 Latest version!	Gallus gallus dystroglycan 1 (DAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa24636	NM_001097540.1 Latest version!	Gallus gallus dystroglycan 1 (DAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAG1 ( XM_025154393.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010161.1 Gallus gallus dystroglycan 1 (DAG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa143863
Clone ID Related Accession (Same CDS sequence)	XM_025154393.1 , XM_025154391.1 , XM_025154392.1
Accession Version	XM_025154393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	dystroglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTACG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGGTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTCGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATTGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCCG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CGCTGCCTCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG ACGACTCCGG GGGGCCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCAGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCAAC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010161.1
CDS	258..2948
Translation	MTVGCVPQPPFLGRTLLPVLLLAASARCHWPSEPAEVVRDWENQLEASMHSVLSDLRETVPAVVGIPDSSAVVGRFFRVSIPTDLIASNGEAVQVSEAGKESLPSWLHWNAESSSLEGLPLDTDKGVHYISVTTLQPFPNGSYVPQAANVFSVEVHQEDHSEPQSVRAAAQEAGDAAPFVCGAEEPVTILTVILDADLTKMTPKQRIELLNRMRSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLSQNSVPNISKVEAPAKEGTMSARLGYPVVGWHIANKKPHLPKRMRRQINATPTPLTAIGPPTTAAQEPPTRIVPTPTSPAIAPPTETTAPPVREPIPLPRKPTVTIRTRGPIVQTPTLGPIQPTRLVEGTGTVSVPIRPTVPGYVEPTAVITPPTTTTKKPRVSTLKPATPSTTDSSTATTRRPTRRPKTPRPTKPPSTTRSTISKLTTASPPTRVRTTASGVPRPWEPNEPPKLTNHIDRVDAWEGTYFEVKIPSDTFYDKEDTTTDKLQLTLKLKEQQMIEENSWVQFNSTSQLMYGMPDRSHVGKHEYFMYATDKGGLFAVDAFEIHVHKRPHGDKSPVKFKARLEGDHSAVANDIHKKIMLVKKLALAFGDRNSSTITVQDIAKGSIVVEWTNNTLPLEPCPREQIRTLSKKIADDSGGPSPAFSNILQPEFKPLNVSVVGSGSCRHIQFVPVTKDGRVISEATPTLAAGKDPEKSSEDDVYLHTVIPAVVVAAILLVAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTIGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_025154393.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus dystroglycan 1 (DAG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154393.1

ORF Insert Sequence:

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTACG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGGTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTCGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATTGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCCG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CGCTGCCTCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG ACGACTCCGG GGGGCCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCAGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCAAC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_025154391.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010159.1 Gallus gallus dystroglycan 1 (DAG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa143863
Clone ID Related Accession (Same CDS sequence)	XM_025154393.1 , XM_025154391.1 , XM_025154392.1
Accession Version	XM_025154391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	dystroglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTACG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGGTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTCGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATTGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCCG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CGCTGCCTCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG ACGACTCCGG GGGGCCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCAGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCAAC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010159.1
CDS	242..2932
Translation	MTVGCVPQPPFLGRTLLPVLLLAASARCHWPSEPAEVVRDWENQLEASMHSVLSDLRETVPAVVGIPDSSAVVGRFFRVSIPTDLIASNGEAVQVSEAGKESLPSWLHWNAESSSLEGLPLDTDKGVHYISVTTLQPFPNGSYVPQAANVFSVEVHQEDHSEPQSVRAAAQEAGDAAPFVCGAEEPVTILTVILDADLTKMTPKQRIELLNRMRSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLSQNSVPNISKVEAPAKEGTMSARLGYPVVGWHIANKKPHLPKRMRRQINATPTPLTAIGPPTTAAQEPPTRIVPTPTSPAIAPPTETTAPPVREPIPLPRKPTVTIRTRGPIVQTPTLGPIQPTRLVEGTGTVSVPIRPTVPGYVEPTAVITPPTTTTKKPRVSTLKPATPSTTDSSTATTRRPTRRPKTPRPTKPPSTTRSTISKLTTASPPTRVRTTASGVPRPWEPNEPPKLTNHIDRVDAWEGTYFEVKIPSDTFYDKEDTTTDKLQLTLKLKEQQMIEENSWVQFNSTSQLMYGMPDRSHVGKHEYFMYATDKGGLFAVDAFEIHVHKRPHGDKSPVKFKARLEGDHSAVANDIHKKIMLVKKLALAFGDRNSSTITVQDIAKGSIVVEWTNNTLPLEPCPREQIRTLSKKIADDSGGPSPAFSNILQPEFKPLNVSVVGSGSCRHIQFVPVTKDGRVISEATPTLAAGKDPEKSSEDDVYLHTVIPAVVVAAILLVAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTIGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_025154391.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus dystroglycan 1 (DAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154391.1

ORF Insert Sequence:

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTACG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGGTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTCGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATTGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCCG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CGCTGCCTCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG ACGACTCCGG GGGGCCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCAGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCAAC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( XM_025154392.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010160.1 Gallus gallus dystroglycan 1 (DAG1), transcript variant X2, mRNA.

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CloneID	OGa143863
Clone ID Related Accession (Same CDS sequence)	XM_025154393.1 , XM_025154391.1 , XM_025154392.1
Accession Version	XM_025154392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	dystroglycan isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTACG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGGTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTCGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATTGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCCG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CGCTGCCTCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG ACGACTCCGG GGGGCCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCAGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCAAC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010160.1
CDS	271..2961
Translation	MTVGCVPQPPFLGRTLLPVLLLAASARCHWPSEPAEVVRDWENQLEASMHSVLSDLRETVPAVVGIPDSSAVVGRFFRVSIPTDLIASNGEAVQVSEAGKESLPSWLHWNAESSSLEGLPLDTDKGVHYISVTTLQPFPNGSYVPQAANVFSVEVHQEDHSEPQSVRAAAQEAGDAAPFVCGAEEPVTILTVILDADLTKMTPKQRIELLNRMRSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLSQNSVPNISKVEAPAKEGTMSARLGYPVVGWHIANKKPHLPKRMRRQINATPTPLTAIGPPTTAAQEPPTRIVPTPTSPAIAPPTETTAPPVREPIPLPRKPTVTIRTRGPIVQTPTLGPIQPTRLVEGTGTVSVPIRPTVPGYVEPTAVITPPTTTTKKPRVSTLKPATPSTTDSSTATTRRPTRRPKTPRPTKPPSTTRSTISKLTTASPPTRVRTTASGVPRPWEPNEPPKLTNHIDRVDAWEGTYFEVKIPSDTFYDKEDTTTDKLQLTLKLKEQQMIEENSWVQFNSTSQLMYGMPDRSHVGKHEYFMYATDKGGLFAVDAFEIHVHKRPHGDKSPVKFKARLEGDHSAVANDIHKKIMLVKKLALAFGDRNSSTITVQDIAKGSIVVEWTNNTLPLEPCPREQIRTLSKKIADDSGGPSPAFSNILQPEFKPLNVSVVGSGSCRHIQFVPVTKDGRVISEATPTLAAGKDPEKSSEDDVYLHTVIPAVVVAAILLVAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTIGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	XM_025154392.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus dystroglycan 1 (DAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154392.1

ORF Insert Sequence:

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTACG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGGTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTCGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATTGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCCG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CGCTGCCTCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG ACGACTCCGG GGGGCCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCAGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCAAC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAG1 ( NM_001097540.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001091009.1 Gallus gallus dystroglycan 1 (DAG1), mRNA.

Price & Availability↑

CloneID	OGa24636
Clone ID Related Accession (Same CDS sequence)	NM_001097540.1
Accession Version	NM_001097540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	dystroglycan precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM690436.1. ##Evidence-Data-START## Transcript exon combination :: AM690436.1, CR524292.1 [ECO:0000332] ##Evidence-Data-END##

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTATG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGCTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTTGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATCGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCTG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CCCTGCCCCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG AAGACTCCGG GGGCGCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCGGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCATC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091009.1
CDS	1..2691
Translation	MTVGCVPQPPFLGRTLLPVLLLAASARCHWPSEPAEVVRDWENQLEASMHSVLSDLRETVPAVVGIPDSSAVVGRFFRVSIPTDLIASNGEAVQVSEAGKESLPSWLHWNAESSSLEGLPLDTDKGVHYISVTTLQPFPNGSYVPQAANVFSVEVHQEDHSEPQSLRAAAQEAGDAAPFVCGAEEPVTILTVILDADLTKMTPKQRIELLNRMRSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLSQNSVPNISKVEAPAKEGTMSARLGYPVVGWHIANKKPHLPKRMRRQINATPTPLTAIGPPTTAAQEPPTRIVPTPTSPAIAPPTETTAPPVREPIPLPRKPTVTIRTRGPIVQTPTLGPIQPTRLVEGTGTVSVPIRPTVPGYVEPTAVITPPTTTTKKPRVSTLKPATPSTTDSSTATTRRPTRRPKTPRPTKPPSTTRSTISKLTTASPPTRVRTTASGVPRPWEPNEPPKLTNHIDRVDAWEGTYFEVKIPSDTFYDKEDTTTDKLQLTLKLKEQQMIEENSWVQFNSTSQLMYGMPDRSHVGKHEYFMYATDKGGLFAVDAFEIHVHKRPHGDKSPVKFKARLEGDHSAVANDIHKKIMLVKKLALAFGDRNSSTITVQDIAKGSIVVEWTNNTLPLEPCPREQIRTLSKKIAEDSGGASPAFSNILQPEFKPLNVSVVGSGSCGHIQFVPVTKDGRVISEATPTLAAGKDPEKSSEDDVYLHTVIPAVVVAAILLVAGIIAMICYRKKRKGKLTIEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSMPETTPLNQDTIGEYTPLRDEDPIAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP

Target ORF information:

RefSeq Version	NM_001097540.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus dystroglycan 1 (DAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097540.1

ORF Insert Sequence:

ATGACTGTTG GATGTGTCCC GCAGCCCCCG TTCCTGGGGA GGACTTTGCT CCCAGTGCTG 
CTGCTCGCAG CCTCAGCTCG CTGCCACTGG CCCAGCGAGC CGGCAGAAGT GGTGCGGGAC 
TGGGAAAACC AGCTGGAGGC CTCCATGCAC TCCGTGCTCT CGGACCTCCG AGAGACTGTG 
CCGGCCGTGG TGGGGATACC AGACAGCTCC GCCGTGGTGG GCCGCTTCTT CAGAGTCTCC 
ATCCCCACAG ATTTAATTGC TTCCAATGGA GAAGCAGTCC AGGTCTCCGA AGCTGGGAAG 
GAGTCTTTGC CTTCGTGGTT ACACTGGAAT GCGGAGAGCA GCTCCCTGGA AGGGCTGCCC 
CTGGACACGG ACAAGGGCGT CCACTACATC TCGGTGACCA CGCTGCAGCC CTTCCCCAAC 
GGCAGCTATG TGCCGCAGGC CGCCAACGTC TTCTCCGTGG AGGTGCACCA GGAGGACCAC 
AGCGAGCCGC AGTCGCTGCG AGCGGCAGCC CAGGAGGCGG GCGATGCCGC GCCGTTCGTG 
TGCGGTGCGG AGGAGCCGGT CACCATCCTG ACCGTCATCC TGGACGCCGA TTTGACAAAA 
ATGACACCAA AGCAGAGGAT CGAACTTTTA AACAGAATGA GGAGCTTCTC AGAGGTGGAG 
CTTCACAACA TGAAGTTGGT TCCTGTTGTA AATAACAGAC TCTTTGACAT GTCGGCCTTC 
ATGGCTGGGC CGGGAAACGC AAAGAAGGTG GTGGAAAACG GGGCCTTACT CTCGTGGAAA 
CTGGGCTGTT CTTTGAGCCA AAACAGCGTC CCCAACATCA GCAAGGTTGA GGCCCCGGCG 
AAAGAAGGGA CTATGTCTGC CCGCCTCGGC TACCCTGTGG TCGGCTGGCA CATCGCTAAC 
AAGAAACCTC ACCTCCCAAA AAGGATGCGA CGGCAGATCA ACGCCACCCC CACGCCTCTG 
ACCGCCATCG GACCCCCCAC CACCGCCGCT CAAGAGCCAC CGACGCGGAT CGTCCCCACC 
CCGACGTCGC CCGCCATCGC TCCCCCCACG GAGACGACGG CCCCGCCGGT CAGGGAGCCC 
ATCCCGCTGC CGAGGAAGCC TACGGTCACC ATCAGGACGA GGGGCCCCAT CGTGCAGACG 
CCCACGCTCG GGCCGATCCA GCCAACCAGG CTGGTGGAAG GCACTGGGAC GGTCTCCGTG 
CCGATTCGCC CCACCGTGCC CGGCTACGTG GAGCCCACCG CGGTGATCAC GCCGCCCACC 
ACCACCACCA AGAAGCCCAG AGTGTCCACT CTGAAGCCTG CCACGCCGTC CACCACGGAT 
TCCTCCACGG CGACGACACG CAGGCCCACC CGCAGGCCCA AGACGCCCCG TCCCACGAAG 
CCTCCCAGCA CCACCCGCTC CACGATCTCC AAGCTGACCA CCGCCTCCCC GCCCACCCGC 
GTGCGCACCA CGGCCAGCGG CGTCCCCCGG CCCTGGGAGC CAAACGAGCC GCCCAAGCTG 
ACGAACCACA TCGACAGGGT GGACGCGTGG GAGGGGACTT ACTTCGAGGT GAAAATACCC 
TCGGATACTT TCTACGACAA GGAGGATACC ACCACTGACA AGCTGCAGCT GACCTTGAAG 
CTGAAGGAGC AGCAAATGAT CGAGGAGAAT TCGTGGGTCC AGTTCAACAG CACCAGCCAG 
CTCATGTACG GCATGCCGGA CCGCAGCCAC GTGGGGAAGC ACGAGTACTT CATGTACGCC 
ACCGACAAAG GGGGGCTGTT TGCCGTGGAC GCTTTTGAAA TCCACGTCCA CAAACGCCCG 
CACGGGGACA AATCCCCGGT GAAGTTCAAG GCCAGGCTGG AAGGAGACCA CAGCGCGGTG 
GCGAACGACA TCCATAAGAA GATCATGCTG GTGAAGAAGC TGGCCCTGGC GTTCGGCGAT 
AGGAACAGCA GCACCATCAC GGTGCAGGAC ATCGCCAAGG GCTCCATCGT GGTGGAATGG 
ACCAACAACA CCCTGCCCCT GGAGCCCTGC CCCCGCGAGC AGATCCGGAC TTTGAGCAAA 
AAGATCGCGG AAGACTCCGG GGGCGCGAGC CCGGCTTTCT CCAACATCCT GCAGCCGGAG 
TTCAAACCTC TGAACGTGTC GGTCGTGGGC TCCGGGAGCT GCGGGCACAT CCAGTTCGTC 
CCCGTGACGA AGGACGGCAG GGTGATCTCG GAGGCGACGC CGACGCTGGC GGCTGGGAAG 
GACCCCGAGA AGAGCAGTGA GGACGATGTG TACCTTCACA CCGTCATCCC CGCCGTGGTG 
GTGGCCGCCA TCCTGCTGGT GGCCGGCATC ATCGCCATGA TCTGCTACCG CAAGAAGAGG 
AAAGGGAAGC TGACCATCGA AGACCAGGCC ACGTTCATTA AGAAGGGCGT GCCCATCATC 
TTCGCCGACG AGCTGGACGA CTCCAAGCCC CCGCCGTCCT CCAGCATGCC CCTCATCCTG 
CAGGAGGAGA AAGCCCCGCT GCCCCCCCCG GAGTACCCCA ACCAGAGCAT GCCCGAGACC 
ACGCCGCTCA ACCAGGACAC CATCGGGGAG TACACGCCGC TGCGGGACGA GGACCCCATC 
GCGCCGCCCT ACCAGCCGCC CCCCCCCTTC ACCGCTCCCA TGGAGGGGAA GGGCTCCCGC 
CCGAAGAACA TGACCCCCTA CCGCTCCCCG CCGCCCTACG TCCCCCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dystroglycan regulates structure, proliferation and differentiation of neuroepithelial cells in the developing vertebrate CNS.
Developmental biology307(1)62-78(2007 Jul)
Schr?der JE,Tegeler MR,Grosshans U,Porten E,Blank M,Lee J,Esapa C,Blake DJ,Kr?ger S

Aberrant glycosylation of alpha-dystroglycan causes defective binding of laminin in the muscle of chicken muscular dystrophy.
FEBS letters579(11)2359-63(2005 Apr)
Saito F,Blank M,Schr?der J,Manya H,Shimizu T,Campbell KP,Endo T,Mizutani M,Kr?ger S,Matsumura K

The effects of post-translational processing on dystroglycan synthesis and trafficking.
FEBS letters555(2)209-16(2003 Dec)
Esapa CT,Bentham GR,Schr?der JE,Kr?ger S,Blake DJ

Agrin is a high-affinity binding protein of dystroglycan in non-muscle tissue.
The Journal of biological chemistry273(1)600-5(1998 Jan)
Gesemann M,Brancaccio A,Schumacher B,Ruegg MA

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DAG1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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