VCL cDNA ORF clone, Galeopterus variegatus(Sunda flying lemur)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	103595124
Full Name	vinculin
Gene Type	protein-coding
Organism	Galeopterus variegatus(Sunda flying lemur)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008578444.1	XP_008576666.1	vinculin isoform X1
XM_008578445.1	XP_008576667.1	vinculin isoform X2

Pathways from BioSystems
Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa106737	XM_008578444.1 Latest version!	Galeopterus variegatus vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa106738	XM_008578445.1 Latest version!	Galeopterus variegatus vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_008578444.1 ) cDNA ORF clone, Galeopterus variegatus(Sunda flying lemur) -> XP_008576666.1 Galeopterus variegatus vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa106737
Clone ID Related Accession (Same CDS sequence)	XM_008578444.1
Accession Version	XM_008578444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-07-21
Organism	Galeopterus variegatus(Sunda flying lemur)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007727800.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Galeopterus variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCGGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGGAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAAATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACGAA GCTTGTCCAA GCAGCCCAGA TGCTTCAGTC AGATCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TTACCTTTGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATCGACGA GAGGCAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTAAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATACGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACCG AAGCCATGAA GAGAGCACTG GCCTCTATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCCGAGACCC CAGTGCCTCC CCAGGGGATG CCGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATGTTCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGGGATGAC ATCCTACGTT CCCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAAGCCCGA 
GCATTGGCCA AACAGGTGGC TACGGCCCTG CAAAACCTGC AGACTAAAAC CAACAGGGCT 
GTAGCCAACA GCAGGCCTGC CAAAGCAGCT GTCCACCTAG AGGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC CACAGTAGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGCCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCCA AGTGTGACCG AGTGGACCAA CTGACAGCCC AGCTGGCTGA TCTGGCTGCC 
AGAGGGGAAG GAGAAAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCAGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GACACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCCCCTCC CGACGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAACCCATTC CGGAAGGCTT 
GGTGCCACAG CAGAGAAAGC GGCTGCTGTT GGCACTGCTA ATAAGTCAAC AGTGGAAGGT 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC GGCTGCTCGC 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCCTATGAAC ATTTTGAGAC TATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACGAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCGAATA TTCAACCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGA 
GAGGCGGTAA AAGCTGCCTC CGATGATTTG AGCAAAACCA TTTCCCCGAT GGTGATGGAC 
GCAAAGGCTG TGGCTGGAAA CATATCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTCGAA CAACTGCGAC TAACAGATGA GCTCGCTCCT 
CCCAAACCAC CCCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCGCC ACCAGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGA AAATGGTCCA GTAAGCCGGG TGTCCCAGCC 
GCAGAGGTAG GTATAGATGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
CTCCCCCCTG ACCTTGAAGA TGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AACCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGTGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG 
ATGTCTCGGC TGGTGAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAAGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGTG AACGGATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAATATCAGT 
GACGAGGAGT CTGAGCAAGC CACAGAGATG CTCGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG AGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008576666.1
CDS	97..3501
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFETHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDDLSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGVPAAEVGIDVVAEADAADAVGFPLPPDLEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_008578444.1
Organism	Galeopterus variegatus(Sunda flying lemur)
Definition	Galeopterus variegatus vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008578444.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCGGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGGAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAAATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACGAA GCTTGTCCAA GCAGCCCAGA TGCTTCAGTC AGATCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TTACCTTTGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATCGACGA GAGGCAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTAAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATACGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACCG AAGCCATGAA GAGAGCACTG GCCTCTATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCCGAGACCC CAGTGCCTCC CCAGGGGATG CCGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATGTTCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGGGATGAC ATCCTACGTT CCCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAAGCCCGA 
GCATTGGCCA AACAGGTGGC TACGGCCCTG CAAAACCTGC AGACTAAAAC CAACAGGGCT 
GTAGCCAACA GCAGGCCTGC CAAAGCAGCT GTCCACCTAG AGGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC CACAGTAGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGCCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCCA AGTGTGACCG AGTGGACCAA CTGACAGCCC AGCTGGCTGA TCTGGCTGCC 
AGAGGGGAAG GAGAAAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCAGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GACACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCCCCTCC CGACGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAACCCATTC CGGAAGGCTT 
GGTGCCACAG CAGAGAAAGC GGCTGCTGTT GGCACTGCTA ATAAGTCAAC AGTGGAAGGT 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC GGCTGCTCGC 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCCTATGAAC ATTTTGAGAC TATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACGAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCGAATA TTCAACCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGA 
GAGGCGGTAA AAGCTGCCTC CGATGATTTG AGCAAAACCA TTTCCCCGAT GGTGATGGAC 
GCAAAGGCTG TGGCTGGAAA CATATCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTCGAA CAACTGCGAC TAACAGATGA GCTCGCTCCT 
CCCAAACCAC CCCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCGCC ACCAGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGA AAATGGTCCA GTAAGCCGGG TGTCCCAGCC 
GCAGAGGTAG GTATAGATGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
CTCCCCCCTG ACCTTGAAGA TGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AACCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGTGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG 
ATGTCTCGGC TGGTGAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAAGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGTG AACGGATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAATATCAGT 
GACGAGGAGT CTGAGCAAGC CACAGAGATG CTCGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG AGAAGCAGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_008578445.1 ) cDNA ORF clone, Galeopterus variegatus(Sunda flying lemur) -> XP_008576667.1 Galeopterus variegatus vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa106738
Clone ID Related Accession (Same CDS sequence)	XM_008578445.1
Accession Version	XM_008578445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-07-21
Organism	Galeopterus variegatus(Sunda flying lemur)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007727800.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Galeopterus variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCGGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGGAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAAATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACGAA GCTTGTCCAA GCAGCCCAGA TGCTTCAGTC AGATCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TTACCTTTGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATCGACGA GAGGCAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTAAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATACGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACCG AAGCCATGAA GAGAGCACTG GCCTCTATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCCGAGACCC CAGTGCCTCC CCAGGGGATG CCGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATGTTCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGGGATGAC ATCCTACGTT CCCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAAGCCCGA 
GCATTGGCCA AACAGGTGGC TACGGCCCTG CAAAACCTGC AGACTAAAAC CAACAGGGCT 
GTAGCCAACA GCAGGCCTGC CAAAGCAGCT GTCCACCTAG AGGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC CACAGTAGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGCCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCCA AGTGTGACCG AGTGGACCAA CTGACAGCCC AGCTGGCTGA TCTGGCTGCC 
AGAGGGGAAG GAGAAAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCAGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GACACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCCCCTCC CGACGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAACCCATTC CGGAAGGCTT 
GGTGCCACAG CAGAGAAAGC GGCTGCTGTT GGCACTGCTA ATAAGTCAAC AGTGGAAGGT 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC GGCTGCTCGC 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCCTATGAAC ATTTTGAGAC TATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACGAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCGAATA TTCAACCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGA 
GAGGCGGTAA AAGCTGCCTC CGATGATTTG AGCAAAACCA TTTCCCCGAT GGTGATGGAC 
GCAAAGGCTG TGGCTGGAAA CATATCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTCGAA CAACTGCGAC TAACAGATGA GCTCGCTCCT 
CCCAAACCAC CCCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCGCC ACCAGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGA AAATGGTCCA GTAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT GAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA AGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAACG GATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAT ATCAGTGACG AGGAGTCTGA GCAAGCCACA 
GAGATGCTCG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGAGAA 
GCAGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008576667.1
CDS	116..3316
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFETHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDDLSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_008578445.1
Organism	Galeopterus variegatus(Sunda flying lemur)
Definition	Galeopterus variegatus vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008578445.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCCG TGGCGGCCGT GCAGGCGGCC GTCAGCAACC TTGTCCGGGT TGGGAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAAATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACGAA GCTTGTCCAA GCAGCCCAGA TGCTTCAGTC AGATCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGCACATCA 
GACCTGCTCC TTACCTTTGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATCGACGA GAGGCAGCAG 
GAGCTGACTC ACCAGGAGCA CCGAGTGATG TTGGTAAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCTGAA 
ATTAATGAGA TCATACGTGT ATTACAACTT ACTTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACCG AAGCCATGAA GAGAGCACTG GCCTCTATAG ACTCCAAACT GAATCAGGCC 
AAAGGTTGGC TCCGAGACCC CAGTGCCTCC CCAGGGGATG CCGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC AGTGGCCATG CAGAAAGCCC AGCAGGTATC TCAGGGTCTG 
GATGTTCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACAAACTCA 
AAGCAGAGCA TTGCAAAGAA GATTGATGCC GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGGCCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGGGATGAC ATCCTACGTT CCCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCCCC AGAAGCCCGA 
GCATTGGCCA AACAGGTGGC TACGGCCCTG CAAAACCTGC AGACTAAAAC CAACAGGGCT 
GTAGCCAACA GCAGGCCTGC CAAAGCAGCT GTCCACCTAG AGGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC CACAGTAGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CTGAAGGGCA TCGCCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTTGCCA AGTGTGACCG AGTGGACCAA CTGACAGCCC AGCTGGCTGA TCTGGCTGCC 
AGAGGGGAAG GAGAAAGTCC TCAGGCGAGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCAGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GACACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCCCCTCC CGACGCACCC 
AATAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAACCCATTC CGGAAGGCTT 
GGTGCCACAG CAGAGAAAGC GGCTGCTGTT GGCACTGCTA ATAAGTCAAC AGTGGAAGGT 
ATTCAGGCGT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTCGTCTC GGCTGCTCGC 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCCTATGAAC ATTTTGAGAC TATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACGAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCGAATA TTCAACCTCA GATGCTGGTC GCTGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC TAAGTTCCGA 
GAGGCGGTAA AAGCTGCCTC CGATGATTTG AGCAAAACCA TTTCCCCGAT GGTGATGGAC 
GCAAAGGCTG TGGCTGGAAA CATATCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTCGAA CAACTGCGAC TAACAGATGA GCTCGCTCCT 
CCCAAACCAC CCCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCGCC ACCAGAGGAG 
AAGGACGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCTCGA AAATGGTCCA GTAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT GAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA AGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAACG GATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAT ATCAGTGACG AGGAGTCTGA GCAAGCCACA 
GAGATGCTCG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGAGAA 
GCAGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G