btd cDNA ORF clone, Esox lucius(northern pike)

Gene
Clones
gRNAs

Summary

Gene Symbol	btd
Entrez Gene ID	105012460
Full Name	biotinidase
Gene Type	protein-coding
Organism	Esox lucius(northern pike)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010873320.2	XP_010871622.1	biotinidase

Pathways from BioSystems
Gene Ontology

The following btd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the btd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEs18506	XM_010873320.2 Latest version!	Esox lucius biotinidase (btd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

btd ( XM_010873320.2 ) cDNA ORF clone, Esox lucius(northern pike) -> XP_010871622.1 Esox lucius biotinidase (btd), mRNA.

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CloneID	OEs18506
Clone ID Related Accession (Same CDS sequence)	XM_010873320.2
Accession Version	XM_010873320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-09
Organism	Esox lucius(northern pike)
Product	biotinidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_025977.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 30, 2017 this sequence version replaced XM_010873320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Esox lucius Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGTCC TTATCCTGGC AGTGGCGGTA GCTGCTCTGC GCCTGTCACA GGCCACATTA 
ACCCGGACCG GGGACGCGAC AGATGACATT CCCGGCCCAT CCTACGTTGC CGCGGTCTAC 
GAGCACAAGG TGATCCTGAA CCCGGAACCG CACGTGCCTC TGTCCCGGTC CGATGCGCTC 
GAGCACATGA TGAGGAACCT GGAGGTGTAT GAGCGGCAGA CCTTGCGGGC GGCGGAACAG 
GGGGCTCAGA TCATAGTATT TCCAGAGGAC GGTATCCACG GGTTCAACTT CAGTCGTCTG 
TCCATATCAG GGTACCTAGA GACCATCCCT GACCCCCTGA CTGAGGACTG GAACCCCTGT 
ACAGAACCCC ACAGGCACAC TTCCACTGAG GTTCTCCAGA GGTTGAGCTG CATGGCTCGA 
CGTCATCGCC TCTACCTAGT GGCCAACATG GCTAGTGTCC AGCCTTGCCC TCTGAACTCC 
AGCCTGGACC TTGACCCCGC CTGTCCGCCC GACGGACGTT GGCAGTTCAA CACAAATGCA 
GTCTTCAAGT CCGATGGGTC CTTGGTTGCG CGCTACCACA AACACAATTT GTACTTTGAG 
AAAGCCTTTG ATGCCCCGCC TAGGCTAGAG GTGGTGACCT TCGACACGCC GTTCGCCGGA 
AAGTTTGGGG TGTTAACCTG CTTCGACATC CTGTTTCATG ACCCCGCTGT CAGACTACTG 
GATCAGGGAG TCCGACAGAT GGTCTACCCC ACGGCTTGGA TGAACCAACT TCCTCTGCTG 
GCCGCGGTTC AGTTCCAGAG GGCTTTCAGT CTGGGCGCTA ACGTGACGCT GCTGGCGGCT 
AACATCCGGT CGGACAGCTT GGGTATGACC GGCAGTGGCG TCTTCACCCC CGACGCTGCC 
ACCTACCATC ACGCCCGTGC CGGGGACCCC GAGGAGGGCA GGCTGCTTGT GGTCAGGGTG 
CCTGTGCTGG ACACGGACTG GCTGGCAACG GAGAACCGGG CGGCGGGGCA GGCGAGAGGA 
GGAACTGTGG AGGCCGGTTC ACCTGTGCCT CCATCTGGCC AAGAGGTGGA CTCTGGGTTC 
TGTCGACTGG GGAACCAGCA ATGCCAGGGC GAACATCTGC CTCATTCCCC CTCTCCCTCG 
CCGCCCTCCC CTTTTACCAC CTCTATGATG TACGATCCGT TCTCCTTCCT GCTGCTCCGC 
GGGTCCGAGG GCAAGCTGAC CGTGTGTGAC GGGCCGCTTT GCTGCCACCT GCAGTACCAA 
CGTTCTCCAT TGGGTGGCAA TGCAGAGCTG TATGCTCTAG GGGCGTTCGC TGGCATTCAC 
ACGGTTAATG GACGATACGC TGTACAGGTG TGCGCATTGG TCCGCTGCTC CGGGTCAGAG 
GCCAGCTCCT GTGGACAGGA AGTGGAGGAG GCAGAGTCCA GATTGGACTT TGTGTTGGAG 
GGAAAGTTCA GAACACGTCA CGTTTACCCG TCAGTGCTAG GCAGTGGAAT GGTCCTAGAA 
CAACCTAACC GGGTTGAGAC GACCCCAGAT GGTCGGGTGA CGATGGCGCA CTCTGGAATG 
ACTGTTGGAC TGGTGACCGC ATGTCTCTAC GGCAGAGTGT ACGATCAGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010871622.1
CDS	520..2133
Translation	MSVLILAVAVAALRLSQATLTRTGDATDDIPGPSYVAAVYEHKVILNPEPHVPLSRSDALEHMMRNLEVYERQTLRAAEQGAQIIVFPEDGIHGFNFSRLSISGYLETIPDPLTEDWNPCTEPHRHTSTEVLQRLSCMARRHRLYLVANMASVQPCPLNSSLDLDPACPPDGRWQFNTNAVFKSDGSLVARYHKHNLYFEKAFDAPPRLEVVTFDTPFAGKFGVLTCFDILFHDPAVRLLDQGVRQMVYPTAWMNQLPLLAAVQFQRAFSLGANVTLLAANIRSDSLGMTGSGVFTPDAATYHHARAGDPEEGRLLVVRVPVLDTDWLATENRAAGQARGGTVEAGSPVPPSGQEVDSGFCRLGNQQCQGEHLPHSPSPSPPSPFTTSMMYDPFSFLLLRGSEGKLTVCDGPLCCHLQYQRSPLGGNAELYALGAFAGIHTVNGRYAVQVCALVRCSGSEASSCGQEVEEAESRLDFVLEGKFRTRHVYPSVLGSGMVLEQPNRVETTPDGRVTMAHSGMTVGLVTACLYGRVYDQD

Target ORF information:

RefSeq Version	XM_010873320.2
Organism	Esox lucius(northern pike)
Definition	Esox lucius biotinidase (btd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010873320.2

ORF Insert Sequence:

ATGTCCGTCC TTATCCTGGC AGTGGCGGTA GCTGCTCTGC GCCTGTCACA GGCCACATTA 
ACCCGGACCG GGGACGCGAC AGATGACATT CCCGGCCCAT CCTACGTTGC CGCGGTCTAC 
GAGCACAAGG TGATCCTGAA CCCGGAACCG CACGTGCCTC TGTCCCGGTC CGATGCGCTC 
GAGCACATGA TGAGGAACCT GGAGGTGTAT GAGCGGCAGA CCTTGCGGGC GGCGGAACAG 
GGGGCTCAGA TCATAGTATT TCCAGAGGAC GGTATCCACG GGTTCAACTT CAGTCGTCTG 
TCCATATCAG GGTACCTAGA GACCATCCCT GACCCCCTGA CTGAGGACTG GAACCCCTGT 
ACAGAACCCC ACAGGCACAC TTCCACTGAG GTTCTCCAGA GGTTGAGCTG CATGGCTCGA 
CGTCATCGCC TCTACCTAGT GGCCAACATG GCTAGTGTCC AGCCTTGCCC TCTGAACTCC 
AGCCTGGACC TTGACCCCGC CTGTCCGCCC GACGGACGTT GGCAGTTCAA CACAAATGCA 
GTCTTCAAGT CCGATGGGTC CTTGGTTGCG CGCTACCACA AACACAATTT GTACTTTGAG 
AAAGCCTTTG ATGCCCCGCC TAGGCTAGAG GTGGTGACCT TCGACACGCC GTTCGCCGGA 
AAGTTTGGGG TGTTAACCTG CTTCGACATC CTGTTTCATG ACCCCGCTGT CAGACTACTG 
GATCAGGGAG TCCGACAGAT GGTCTACCCC ACGGCTTGGA TGAACCAACT TCCTCTGCTG 
GCCGCGGTTC AGTTCCAGAG GGCTTTCAGT CTGGGCGCTA ACGTGACGCT GCTGGCGGCT 
AACATCCGGT CGGACAGCTT GGGTATGACC GGCAGTGGCG TCTTCACCCC CGACGCTGCC 
ACCTACCATC ACGCCCGTGC CGGGGACCCC GAGGAGGGCA GGCTGCTTGT GGTCAGGGTG 
CCTGTGCTGG ACACGGACTG GCTGGCAACG GAGAACCGGG CGGCGGGGCA GGCGAGAGGA 
GGAACTGTGG AGGCCGGTTC ACCTGTGCCT CCATCTGGCC AAGAGGTGGA CTCTGGGTTC 
TGTCGACTGG GGAACCAGCA ATGCCAGGGC GAACATCTGC CTCATTCCCC CTCTCCCTCG 
CCGCCCTCCC CTTTTACCAC CTCTATGATG TACGATCCGT TCTCCTTCCT GCTGCTCCGC 
GGGTCCGAGG GCAAGCTGAC CGTGTGTGAC GGGCCGCTTT GCTGCCACCT GCAGTACCAA 
CGTTCTCCAT TGGGTGGCAA TGCAGAGCTG TATGCTCTAG GGGCGTTCGC TGGCATTCAC 
ACGGTTAATG GACGATACGC TGTACAGGTG TGCGCATTGG TCCGCTGCTC CGGGTCAGAG 
GCCAGCTCCT GTGGACAGGA AGTGGAGGAG GCAGAGTCCA GATTGGACTT TGTGTTGGAG 
GGAAAGTTCA GAACACGTCA CGTTTACCCG TCAGTGCTAG GCAGTGGAAT GGTCCTAGAA 
CAACCTAACC GGGTTGAGAC GACCCCAGAT GGTCGGGTGA CGATGGCGCA CTCTGGAATG 
ACTGTTGGAC TGGTGACCGC ATGTCTCTAC GGCAGAGTGT ACGATCAGGA CTAG