LOC114656385 cDNA ORF clone,

Gene
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gRNAs

The following LOC114656385 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC114656385 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORe54779	XM_028807988.1 Latest version!	Erpetoichthys calabaricus TGF-beta receptor type-2-like (LOC114656385), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC114656385 ( XM_028807988.1 ) cDNA ORF clone, -> XP_028663821.1 Erpetoichthys calabaricus TGF-beta receptor type-2-like (LOC114656385), mRNA.

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CloneID	ORe54779
Clone ID Related Accession (Same CDS sequence)	XM_028807988.1
Accession Version	XM_028807988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-20
Organism	Erpetoichthys calabaricus(reedfish)
Product	TGF-beta receptor type-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041401.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Erpetoichthys calabaricus Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCA GCTTCGTGAT CGCAACAATT TTACTATCTG TTGCTTTACC TCTCCATGCC 
CTGGCTTTTA CTACTGTGAA TTCCAACTTT TGCAAATGGT GTGACTGGTT CACAGGCCCA 
GTGTGTGAGG GGAACGTGTG CACCTCAAAC TGCAACTTCA GCTCCTTCTG TGAACGTACT 
GAAGAAATCT GCGTATCTGT ATGGAAACAG AACAATGAGA GTATCAGCGT CAGGACCCTG 
TGTCATGACC CTTACCTTCC CTTGGAAAAC ATGAAGGTGC CAAATTATAC GAGAAACGAG 
TGCCGCATGG TGCAGCAGCA GACCGACAAG GGAATATATT ATGTCTGTGG GTGTGTAGAA 
GAACACGAAT GCAATGATGT GCTCATCTTT GAAAAGAACT TCAATGGATT TGCAAGACTA 
CAGAGTGAAG AAGTCATTCC TGTTGCAGTG ATCAGCCTGC TTCCTCCTAT TTTGTTGACT 
ATCATGGCTA CAATGGCGTT TTATTTGTAC CGCACTCGTC AGCAAAGGAA GGAGCCCAAA 
GACTGGCCAC CCAAACAGTG CCAAAGCCAA GCGTTAGGCC TTCTAGAGAA TAGAGAACCT 
ACAGAATTTG AAGGGAAAAT GTCCGTTATA ACAGACGATG GCCACTCGGA CATTTCCTCC 
ACATGTGCCA ACAATATCAA TCACAACACC GAACTTCTGC CAATCGAACT TGAAGTAATG 
GTAGGGAAAG GCCGGTTTGC TGAAGTTTGG AGGGCCAGGC TCAACCACAA TGCTTCAGGC 
CAATACGAGA CAGTGGCCGT CAAAATATTC CCTCCAGAGG AATACTTGTC TTGGAAGAGC 
GAGCGGAAAA TCTTCTCCGA TGCAAACCTG AAACATGAGA GTGTTCTACA ATTCCTGACT 
GCGGAAGAAA GGGGAATGGG TTTGCAGAAG CAATACTGGC TTATTACTGC CTATCACAGC 
TTGGGCAACT TGCAAGAATA TTTGACCTCT CATGTTCTAA GCTGGACAGA ACTCTGCTTA 
ATGGCCAGTT CCCTGGTGAA TGGCGTAGCT CATCTACACA GCGACTATAC TGTGTGTGGA 
ACACCCAAAA TAGCAATCGC CCATCGTGAT ATCAAAAGCT CCAATATCGT GGTTAAGAGC 
ACGACAGAAT GTGTCCTGTG TGACTTTGGG CTAGCACTTC GATTGGATCC TTCACTTACT 
GCAAATGATT TTGCCAACAG TGGGCAGGTT GGCACAGCCC GGTACATGGC TCCTGAGGTT 
CTCGAGTCTC GTGTGAACCT TGAAGATCTG GAATCATTCA AACAAATGGA TGTTTATTCA 
ATGGCTCTTG TGCTGTGGGA GATGGCTTCT CGTTGCGACA TCACTGGTGA GGTCAAAAGC 
TATGAACTTC CGTTTGGATC TAAAGTATGT GAACATCCAT GTGTGGAAAG CATGAGAGAC 
ATTGTTCTCA GAGATCGTGG AAGACCAGAC ATCCCAAACA GCTGGATGAT ACACAAGGGC 
ATGAAGTTCT TTTGTGACAC AATCATCGAG TGCTGGGATC ATGACCCCGA GGCTCGTCTA 
ACGGCTCACT GTGTTGCTGA ACGTTTCAGC ATGCTGGGGC AGGAAGGATG CGAGGACATT 
CTGAACAACA ACAGCCAAAA CATCAGCGCA ATGGAACCCT GGAAGCCAGT TGTTGAAGGT 
CTTCCCCAAG AAACTGTGCC TTGTGTGGTT GCTCTACCAG TGAGATTGGT AACTGATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028663821.1
CDS	143..1885
Translation	MESSFVIATILLSVALPLHALAFTTVNSNFCKWCDWFTGPVCEGNVCTSNCNFSSFCERTEEICVSVWKQNNESISVRTLCHDPYLPLENMKVPNYTRNECRMVQQQTDKGIYYVCGCVEEHECNDVLIFEKNFNGFARLQSEEVIPVAVISLLPPILLTIMATMAFYLYRTRQQRKEPKDWPPKQCQSQALGLLENREPTEFEGKMSVITDDGHSDISSTCANNINHNTELLPIELEVMVGKGRFAEVWRARLNHNASGQYETVAVKIFPPEEYLSWKSERKIFSDANLKHESVLQFLTAEERGMGLQKQYWLITAYHSLGNLQEYLTSHVLSWTELCLMASSLVNGVAHLHSDYTVCGTPKIAIAHRDIKSSNIVVKSTTECVLCDFGLALRLDPSLTANDFANSGQVGTARYMAPEVLESRVNLEDLESFKQMDVYSMALVLWEMASRCDITGEVKSYELPFGSKVCEHPCVESMRDIVLRDRGRPDIPNSWMIHKGMKFFCDTIIECWDHDPEARLTAHCVAERFSMLGQEGCEDILNNNSQNISAMEPWKPVVEGLPQETVPCVVALPVRLVTDV

Target ORF information:

RefSeq Version	XM_028807988.1
Organism	Erpetoichthys calabaricus(reedfish)
Definition	Erpetoichthys calabaricus TGF-beta receptor type-2-like (LOC114656385), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028807988.1

ORF Insert Sequence:

ATGGAGAGCA GCTTCGTGAT CGCAACAATT TTACTATCTG TTGCTTTACC TCTCCATGCC 
CTGGCTTTTA CTACTGTGAA TTCCAACTTT TGCAAATGGT GTGACTGGTT CACAGGCCCA 
GTGTGTGAGG GGAACGTGTG CACCTCAAAC TGCAACTTCA GCTCCTTCTG TGAACGTACT 
GAAGAAATCT GCGTATCTGT ATGGAAACAG AACAATGAGA GTATCAGCGT CAGGACCCTG 
TGTCATGACC CTTACCTTCC CTTGGAAAAC ATGAAGGTGC CAAATTATAC GAGAAACGAG 
TGCCGCATGG TGCAGCAGCA GACCGACAAG GGAATATATT ATGTCTGTGG GTGTGTAGAA 
GAACACGAAT GCAATGATGT GCTCATCTTT GAAAAGAACT TCAATGGATT TGCAAGACTA 
CAGAGTGAAG AAGTCATTCC TGTTGCAGTG ATCAGCCTGC TTCCTCCTAT TTTGTTGACT 
ATCATGGCTA CAATGGCGTT TTATTTGTAC CGCACTCGTC AGCAAAGGAA GGAGCCCAAA 
GACTGGCCAC CCAAACAGTG CCAAAGCCAA GCGTTAGGCC TTCTAGAGAA TAGAGAACCT 
ACAGAATTTG AAGGGAAAAT GTCCGTTATA ACAGACGATG GCCACTCGGA CATTTCCTCC 
ACATGTGCCA ACAATATCAA TCACAACACC GAACTTCTGC CAATCGAACT TGAAGTAATG 
GTAGGGAAAG GCCGGTTTGC TGAAGTTTGG AGGGCCAGGC TCAACCACAA TGCTTCAGGC 
CAATACGAGA CAGTGGCCGT CAAAATATTC CCTCCAGAGG AATACTTGTC TTGGAAGAGC 
GAGCGGAAAA TCTTCTCCGA TGCAAACCTG AAACATGAGA GTGTTCTACA ATTCCTGACT 
GCGGAAGAAA GGGGAATGGG TTTGCAGAAG CAATACTGGC TTATTACTGC CTATCACAGC 
TTGGGCAACT TGCAAGAATA TTTGACCTCT CATGTTCTAA GCTGGACAGA ACTCTGCTTA 
ATGGCCAGTT CCCTGGTGAA TGGCGTAGCT CATCTACACA GCGACTATAC TGTGTGTGGA 
ACACCCAAAA TAGCAATCGC CCATCGTGAT ATCAAAAGCT CCAATATCGT GGTTAAGAGC 
ACGACAGAAT GTGTCCTGTG TGACTTTGGG CTAGCACTTC GATTGGATCC TTCACTTACT 
GCAAATGATT TTGCCAACAG TGGGCAGGTT GGCACAGCCC GGTACATGGC TCCTGAGGTT 
CTCGAGTCTC GTGTGAACCT TGAAGATCTG GAATCATTCA AACAAATGGA TGTTTATTCA 
ATGGCTCTTG TGCTGTGGGA GATGGCTTCT CGTTGCGACA TCACTGGTGA GGTCAAAAGC 
TATGAACTTC CGTTTGGATC TAAAGTATGT GAACATCCAT GTGTGGAAAG CATGAGAGAC 
ATTGTTCTCA GAGATCGTGG AAGACCAGAC ATCCCAAACA GCTGGATGAT ACACAAGGGC 
ATGAAGTTCT TTTGTGACAC AATCATCGAG TGCTGGGATC ATGACCCCGA GGCTCGTCTA 
ACGGCTCACT GTGTTGCTGA ACGTTTCAGC ATGCTGGGGC AGGAAGGATG CGAGGACATT 
CTGAACAACA ACAGCCAAAA CATCAGCGCA ATGGAACCCT GGAAGCCAGT TGTTGAAGGT 
CTTCCCCAAG AAACTGTGCC TTGTGTGGTT GCTCTACCAG TGAGATTGGT AACTGATGTG 
TAA