VARS cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS
Entrez Gene ID	103126271
Full Name	valyl-tRNA synthetase
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007537166.2	XP_007537228.1	valine--tRNA ligase

Pathways from BioSystems
Gene Ontology

The following VARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr25186	XM_007537166.2 Latest version!	Erinaceus europaeus valyl-tRNA synthetase (VARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS ( XM_007537166.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007537228.1 Erinaceus europaeus valyl-tRNA synthetase (VARS), mRNA.

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CloneID	OEr25186
Clone ID Related Accession (Same CDS sequence)	XM_007537166.2
Accession Version	XM_007537166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804941.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007537166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCC TCTACGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCGCGCT TCGGGGAGGC GGGCGACGGC CCGGGCTGGG GAGGGGCTCC CCCCCGCGTC 
TGCCTCCAGC CGCCCCCGGC GAGCAGGACC CCCTTCCCGC CACCGCGCCT GCCCGCTTTG 
GAGCAAGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GCTGCTGTGG 
CCGGCCGGCT TGGGGGGCCC CGGAGGGAGC CGGGCAGCCG TCCTCGTCCA ACAGTGGGTG 
AGTTATGCCG ACACCGAGCT CATACCTGCA GCCTGTGGGG CGACGCTGCC GTCCTTGGGA 
CTCCGAAACT CTGCCCAGGA CCCCCAGGCT GCACTGGGGA TCCTGGGCAG GGCCCTGAGC 
CCCTTGGAAG AGTGGCTTCG CTTGCACACC TATCTGGCTG GGGAAGCCCC CACTCTGGCT 
GACTTGGCAG CTGTCACAGC TTTGCTGCTA CCTTTCAGAT ATGTCCTGGG CCCTTCTGCC 
CGTCGCATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA ACCAGAATTC 
CGGGCTGTTT TGGGAGAAGT GGTTTTGTAT TCAGGCACCA GGCCTCTGTC CCAACAGCCA 
GGCCCTGAGG TCCCTGGACC TCCAAAGACA GCTGCTCAAC TCAAGAAAGA GGCAAAGAAG 
CGGGAGAAAC TGGAGAAATT CCAACAGAAG CAGAAGTTTC AACAGCAACA GCCACCTCCA 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAAAAACGAG ATCCAGGGGT CATTACCTAT 
GACCTCCAGA CCCCACCTGG GGAAAAGAAA GATGTCAGTG GTACCATGCC TGACTCCTAC 
AGCCCACAGT ATGTGGAAGC TGCCTGGTAC TCGTGGTGGG AGCACCAGGG CTTCTTCAAG 
CCAGAATATG GGCGGTCTAG TGTATCAGCT CCAAATCCCC GAGGCATCTT CATGATGTGC 
ATTCCACCCC CCAACGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTTAC CAATGCCATT 
CAGGACTCCC TGACCAGATG GCACCGAATG CGGGGGGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCACG CAGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAATTGTG GCGTGAACAG 
GGCCTGAATA GACACCAGCT GGGCCGAGAG GCCTTTCTCC AAGAGGTCTG GAAGTGGAAG 
GAGGAAAAAG GGGACAGGAT CTACCACCAG CTAAGGAAGC TAGGCAGTTC CCTGGACTGG 
AATCGAGCTT GTTTCACCAT GGACCCTAAA CTGTCAGCAG CCGTGACGGA GGCCTTTGTC 
CAGCTCCATG AGGAAGGAGT CATCTACCGT AGTACCCGTC TTGTCAACTG GTCCTGCACC 
CTCAACTCTG CCATCTCTGA CATCGAGGTG GATAAGAAGG AGCTGACAGG ACGCACTCTG 
CTCTCTGTGC CTGGCTACAA GGAGAAAGTT GAGTTTGGGG TTCTCATCTC CTTTGCCTAT 
AAGGTCCAAG GATCAGACAG CGAAGAGGAG GTGGTAGTGG CAACAACTCG GATTGAGACA 
ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAGGATT CCAGATACCA GCATCTGAAG 
GGAAAGAGCG TCATTCACCC ATTTGTGTCT CGGAGCCTCC CCATTGTCTT TGATGATTTC 
GTGGACATGG AGTTTGGCAC AGGTGCGGTG AAGATCACCC CTGCCCACGA CCAGAATGAC 
TATGAGGTGG GCCAGCGTCA TGGACTGGAG GCCATCAGCA TCATAGACGC CCGAGGGTCC 
CTAGTCAATG TGCCTCCACC ATTCCTGGGC CTGCCCAGGT TTGAGGCCAG AAAGGCTGTC 
CTGGCTGCGC TGAAGGAACG GGGACTGTTC CGTGGCATTC AGGACAACCC CATGGTGGTC 
CCGCTTTGCA ACCGGTCCAA GGATGTGGTG GAACCTCTGC TGCGGCCACA GTGGTACGTG 
CGCTGCGGGG AGATGGCCCA AGCCGCCAGC GCTGCTGTGA CTCGGTGTGA CCTCCGGATC 
CTGCCTGAGG CCCATCAGAG AACATGGCAT GCCTGGATGG ACAACATCCG GGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGCCACCGC ATCCCAGCCT ACTTCATCAC TGTCAACGAC 
CCTGCCGTGC CCCCAGGGGA GGACCCTGAT GGACGGTACT GGGTGAGTGG GCGCAGCGAG 
GCCGAGGCCC AAGAGAAGGC AGCCAAGGAG TTTGGAGTTT CCCTTGACAA GATCAGTCTC 
CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC 
CTGGGATGGC CCAACCAGTC AGAAGATCTG AGTGTATTCT ACCCAGGGAC GCTGCTGGAG 
ACAGGCTATG ACATTCTCTT CTTCTGGGTG GCCAGGATGG TCATGCTCGG CCTGAAGCTC 
ACGGGCAAAC TGCCCTTCAG AGAGGTCTAC CTCCATGCCA TTGTGCGAGA CGCCCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGACCCCC TGGATGTTAT TCATGGAGTC 
TCCCTGCAGG GGCTCCATGA TCAGTTACTG AACAGCAACC TGGATCCTAG TGAGGTGGAG 
AAGGCTAAAG AAGGACAGAA GACAGACTTC CCATCAGGGA TTCCTGAGTG TGGCACCGAT 
GCCCTCCGTT TCGGATTGTG TGCATATACA TCCCAGGGTC GAGACATCAA CCTGGATGTG 
AACCGGATCC TGGGGTACCG TCACTTCTGC AACAAGCTGT GGAATGCCAC CAAGTTTGCC 
CTTCGTGGAC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CTGAGCCTGG AGGCCACGAG 
AGCCTGGTGG ATCGCTGGAT CAGAAGCCGT CTGGCAGAGG CTGTGAGACT CAGCAACCAA 
GGCTTCCAGG CCTACGATTT CCCAGCTGTC ACTACTGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTATCT GGAGTGCCTA AAGCCTGTGC TGAATGGGGT GGACCAGGTG 
GCTGCTGAGT GTGCCTGTCA GACCCTCTAC ACCTGCCTGG ACGTTGGTCT GCGTCTGCTC 
TCACCTTTCA TGCCTTTTGT GACCGAGGAA CTGTTCCAGA GGCTTCCCCG GCGGATACCA 
CAAGCTCCCC CCAGCCTCTG TGTTACCTCC TATCCGGAGC CCTCAGAGTG CTCCTGGAAG 
GACTCAGCAG CTGAAGCTGC TATGGAGCTG GCCCTGAGCA TCACACGAGC TGTGCGCTCC 
CTGCGTGCCG ACTACAACCT CACTCGGACC CGGCCTGACT GTTTCCTGGA AGTGGCTGAT 
GAAGCAACAG GCTCCCTGGC ATCAGCAGTG TCGGGCTATG TGCAGGCACT GGCCAGTGCC 
GGTGTGGTAG CTGTCCTGGC CCTGGGGGCT CCTGCACCCC AGGGCTGTGC TGTGGCCCTG 
GCCTCTGACC GCTGCTCAGT CCACCTGCAG CTACAGGGAC TGGTGGACCC AGCCCGGGAG 
CTGGGCAAGC TGCAGACCAA GCGTAGTGAG GCACAGAAGC AGGTTCAGCG TCTGCAGGAG 
CGCCGTGCTG CCGCGGGCTA CCCTGTCAAG GTGCCCCTGG AAGTCCAGAA GGCAGATGAA 
GCCAAGCTCC AGCAGACAGA AGCAGAACTC AGGAAGATGG ATGAGGCCAT TGCCCTATTT 
CAGAAGATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007537228.1
CDS	143..3937
Translation	MSILYVSPHPDAFPSLRALIAARFGEAGDGPGWGGAPPRVCLQPPPASRTPFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPSLGLRNSAQDPQAALGILGRALSPLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLGPSARRIWGNVTRWFTTCVQQPEFRAVLGEVVLYSGTRPLSQQPGPEVPGPPKTAAQLKKEAKKREKLEKFQQKQKFQQQQPPPGEKKPKPEKREKRDPGVITYDLQTPPGEKKDVSGTMPDSYSPQYVEAAWYSWWEHQGFFKPEYGRSSVSAPNPRGIFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLNRHQLGREAFLQEVWKWKEEKGDRIYHQLRKLGSSLDWNRACFTMDPKLSAAVTEAFVQLHEEGVIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKEKVEFGVLISFAYKVQGSDSEEEVVVATTRIETMLGDVAVAVHPKDSRYQHLKGKSVIHPFVSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHGLEAISIIDARGSLVNVPPPFLGLPRFEARKAVLAALKERGLFRGIQDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRCDLRILPEAHQRTWHAWMDNIRDWCISRQLWWGHRIPAYFITVNDPAVPPGEDPDGRYWVSGRSEAEAQEKAAKEFGVSLDKISLQQDEDVLDTWFSSGLFPFSILGWPNQSEDLSVFYPGTLLETGYDILFFWVARMVMLGLKLTGKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLHDQLLNSNLDPSEVEKAKEGQKTDFPSGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSEPGGHESLVDRWIRSRLAEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECACQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRIPQAPPSLCVTSYPEPSECSWKDSAAEAAMELALSITRAVRSLRADYNLTRTRPDCFLEVADEATGSLASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSVHLQLQGLVDPARELGKLQTKRSEAQKQVQRLQERRAAAGYPVKVPLEVQKADEAKLQQTEAELRKMDEAIALFQKML

Target ORF information:

RefSeq Version	XM_007537166.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus valyl-tRNA synthetase (VARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007537166.2

ORF Insert Sequence:

ATGTCCATCC TCTACGTGTC TCCTCACCCG GACGCCTTCC CCAGCCTCCG AGCCCTCATA 
GCCGCGCGCT TCGGGGAGGC GGGCGACGGC CCGGGCTGGG GAGGGGCTCC CCCCCGCGTC 
TGCCTCCAGC CGCCCCCGGC GAGCAGGACC CCCTTCCCGC CACCGCGCCT GCCCGCTTTG 
GAGCAAGGGC CCGGCGGGCT CTGGGTGTGG GGGGCCACCG CCGTGGCCCA GCTGCTGTGG 
CCGGCCGGCT TGGGGGGCCC CGGAGGGAGC CGGGCAGCCG TCCTCGTCCA ACAGTGGGTG 
AGTTATGCCG ACACCGAGCT CATACCTGCA GCCTGTGGGG CGACGCTGCC GTCCTTGGGA 
CTCCGAAACT CTGCCCAGGA CCCCCAGGCT GCACTGGGGA TCCTGGGCAG GGCCCTGAGC 
CCCTTGGAAG AGTGGCTTCG CTTGCACACC TATCTGGCTG GGGAAGCCCC CACTCTGGCT 
GACTTGGCAG CTGTCACAGC TTTGCTGCTA CCTTTCAGAT ATGTCCTGGG CCCTTCTGCC 
CGTCGCATCT GGGGTAATGT GACTCGCTGG TTTACCACAT GTGTCCAGCA ACCAGAATTC 
CGGGCTGTTT TGGGAGAAGT GGTTTTGTAT TCAGGCACCA GGCCTCTGTC CCAACAGCCA 
GGCCCTGAGG TCCCTGGACC TCCAAAGACA GCTGCTCAAC TCAAGAAAGA GGCAAAGAAG 
CGGGAGAAAC TGGAGAAATT CCAACAGAAG CAGAAGTTTC AACAGCAACA GCCACCTCCA 
GGGGAGAAGA AACCAAAACC AGAGAAGAGG GAAAAACGAG ATCCAGGGGT CATTACCTAT 
GACCTCCAGA CCCCACCTGG GGAAAAGAAA GATGTCAGTG GTACCATGCC TGACTCCTAC 
AGCCCACAGT ATGTGGAAGC TGCCTGGTAC TCGTGGTGGG AGCACCAGGG CTTCTTCAAG 
CCAGAATATG GGCGGTCTAG TGTATCAGCT CCAAATCCCC GAGGCATCTT CATGATGTGC 
ATTCCACCCC CCAACGTGAC AGGCTCCCTG CACCTGGGCC ATGCACTTAC CAATGCCATT 
CAGGACTCCC TGACCAGATG GCACCGAATG CGGGGGGAGA CCACCCTGTG GAACCCTGGC 
TGTGACCACG CAGGCATTGC CACCCAGGTG GTGGTGGAGA AGAAATTGTG GCGTGAACAG 
GGCCTGAATA GACACCAGCT GGGCCGAGAG GCCTTTCTCC AAGAGGTCTG GAAGTGGAAG 
GAGGAAAAAG GGGACAGGAT CTACCACCAG CTAAGGAAGC TAGGCAGTTC CCTGGACTGG 
AATCGAGCTT GTTTCACCAT GGACCCTAAA CTGTCAGCAG CCGTGACGGA GGCCTTTGTC 
CAGCTCCATG AGGAAGGAGT CATCTACCGT AGTACCCGTC TTGTCAACTG GTCCTGCACC 
CTCAACTCTG CCATCTCTGA CATCGAGGTG GATAAGAAGG AGCTGACAGG ACGCACTCTG 
CTCTCTGTGC CTGGCTACAA GGAGAAAGTT GAGTTTGGGG TTCTCATCTC CTTTGCCTAT 
AAGGTCCAAG GATCAGACAG CGAAGAGGAG GTGGTAGTGG CAACAACTCG GATTGAGACA 
ATGCTGGGAG ATGTGGCTGT AGCTGTGCAC CCCAAGGATT CCAGATACCA GCATCTGAAG 
GGAAAGAGCG TCATTCACCC ATTTGTGTCT CGGAGCCTCC CCATTGTCTT TGATGATTTC 
GTGGACATGG AGTTTGGCAC AGGTGCGGTG AAGATCACCC CTGCCCACGA CCAGAATGAC 
TATGAGGTGG GCCAGCGTCA TGGACTGGAG GCCATCAGCA TCATAGACGC CCGAGGGTCC 
CTAGTCAATG TGCCTCCACC ATTCCTGGGC CTGCCCAGGT TTGAGGCCAG AAAGGCTGTC 
CTGGCTGCGC TGAAGGAACG GGGACTGTTC CGTGGCATTC AGGACAACCC CATGGTGGTC 
CCGCTTTGCA ACCGGTCCAA GGATGTGGTG GAACCTCTGC TGCGGCCACA GTGGTACGTG 
CGCTGCGGGG AGATGGCCCA AGCCGCCAGC GCTGCTGTGA CTCGGTGTGA CCTCCGGATC 
CTGCCTGAGG CCCATCAGAG AACATGGCAT GCCTGGATGG ACAACATCCG GGACTGGTGC 
ATCTCCCGGC AGCTGTGGTG GGGCCACCGC ATCCCAGCCT ACTTCATCAC TGTCAACGAC 
CCTGCCGTGC CCCCAGGGGA GGACCCTGAT GGACGGTACT GGGTGAGTGG GCGCAGCGAG 
GCCGAGGCCC AAGAGAAGGC AGCCAAGGAG TTTGGAGTTT CCCTTGACAA GATCAGTCTC 
CAGCAAGATG AGGATGTATT GGATACCTGG TTCTCCTCTG GCCTCTTCCC CTTCTCCATC 
CTGGGATGGC CCAACCAGTC AGAAGATCTG AGTGTATTCT ACCCAGGGAC GCTGCTGGAG 
ACAGGCTATG ACATTCTCTT CTTCTGGGTG GCCAGGATGG TCATGCTCGG CCTGAAGCTC 
ACGGGCAAAC TGCCCTTCAG AGAGGTCTAC CTCCATGCCA TTGTGCGAGA CGCCCACGGC 
CGGAAGATGA GCAAGTCTCT AGGCAATGTC ATCGACCCCC TGGATGTTAT TCATGGAGTC 
TCCCTGCAGG GGCTCCATGA TCAGTTACTG AACAGCAACC TGGATCCTAG TGAGGTGGAG 
AAGGCTAAAG AAGGACAGAA GACAGACTTC CCATCAGGGA TTCCTGAGTG TGGCACCGAT 
GCCCTCCGTT TCGGATTGTG TGCATATACA TCCCAGGGTC GAGACATCAA CCTGGATGTG 
AACCGGATCC TGGGGTACCG TCACTTCTGC AACAAGCTGT GGAATGCCAC CAAGTTTGCC 
CTTCGTGGAC TTGGGAAGGG TTTTGTGCCC TCACCCACCT CTGAGCCTGG AGGCCACGAG 
AGCCTGGTGG ATCGCTGGAT CAGAAGCCGT CTGGCAGAGG CTGTGAGACT CAGCAACCAA 
GGCTTCCAGG CCTACGATTT CCCAGCTGTC ACTACTGCCC AGTACAGCTT CTGGCTCTAT 
GAGCTCTGTG ATGTCTATCT GGAGTGCCTA AAGCCTGTGC TGAATGGGGT GGACCAGGTG 
GCTGCTGAGT GTGCCTGTCA GACCCTCTAC ACCTGCCTGG ACGTTGGTCT GCGTCTGCTC 
TCACCTTTCA TGCCTTTTGT GACCGAGGAA CTGTTCCAGA GGCTTCCCCG GCGGATACCA 
CAAGCTCCCC CCAGCCTCTG TGTTACCTCC TATCCGGAGC CCTCAGAGTG CTCCTGGAAG 
GACTCAGCAG CTGAAGCTGC TATGGAGCTG GCCCTGAGCA TCACACGAGC TGTGCGCTCC 
CTGCGTGCCG ACTACAACCT CACTCGGACC CGGCCTGACT GTTTCCTGGA AGTGGCTGAT 
GAAGCAACAG GCTCCCTGGC ATCAGCAGTG TCGGGCTATG TGCAGGCACT GGCCAGTGCC 
GGTGTGGTAG CTGTCCTGGC CCTGGGGGCT CCTGCACCCC AGGGCTGTGC TGTGGCCCTG 
GCCTCTGACC GCTGCTCAGT CCACCTGCAG CTACAGGGAC TGGTGGACCC AGCCCGGGAG 
CTGGGCAAGC TGCAGACCAA GCGTAGTGAG GCACAGAAGC AGGTTCAGCG TCTGCAGGAG 
CGCCGTGCTG CCGCGGGCTA CCCTGTCAAG GTGCCCCTGG AAGTCCAGAA GGCAGATGAA 
GCCAAGCTCC AGCAGACAGA AGCAGAACTC AGGAAGATGG ATGAGGCCAT TGCCCTATTT 
CAGAAGATGC TGTGA