PTCH2 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTCH2
Entrez Gene ID	103120565
Full Name	patched 2
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016191653.1	XP_016047139.1	protein patched homolog 2 isoform X1
XM_016191654.1	XP_016047140.1	protein patched homolog 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following PTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr19089	XM_016191653.1 Latest version!	Erinaceus europaeus patched 2 (PTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr19090	XM_016191654.1 Latest version!	Erinaceus europaeus patched 2 (PTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTCH2 ( XM_016191653.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016047139.1 Erinaceus europaeus patched 2 (PTCH2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEr19089
Clone ID Related Accession (Same CDS sequence)	XM_016191653.1
Accession Version	XM_016191653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804424.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGATAC AGACCCCACG ACAGGAGGGG GAGAATGTCC TCACACCTGA GGCTCTCGGC 
CTCCACCTCC AGGCAGCCCT CACTGCTAGT AAAGTGCAAG TATCACTCTA TGGAAAATCC 
TGGGATCTAA ACAAAATCTG CTACAAGTCA GGAGTGCCCC TCATTGAAAA CGGAATGATT 
GAGCGGATGA TTGAGAAGCT GTTTCCATGT GTGATCCTCA CCCCCCTCGA CTGCTTCTGG 
GAGGGAGCCA AACTCCAAGG GGGCTCCGCC TACTTACCGG GTCGGCCTGA CATCCAGTGG 
ACCAACCTGG ACCCGGAGCA GCTGCTAGAG GAGCTGGGCC CCTTCGCCTC CCTTGAGGGC 
TTCCGAGAGC TGCTCGACAA GGCACAGGTG GGCCAGGCCT ACGTGGGGAG GCCCTGTCTG 
CACCCGGATG ACCTTCACTG CCCTCCTAGC GCCCCTAATC ACCACAGCAG GCAGGCTCCC 
AATGTGGCTC AGGAGCTGGA TGGAGGCTGC CATGGCTTCT CTCACAAGTT CATGCATTGG 
CAGGAGGAGT TGCTGCTGGG AGGCATGACC AGAGACCCCC AGGGACAGCT ACTAAGGGCA 
GAGGCTCTGC AAACCACCTT CCTGCTGATG AGTCCCCGGC AGCTGTATGA GCATTTCCGG 
GGAGACTACC AGACACACGA CATCGGCTGG AGTGAGGAGC AGGCTGCCAC CGTGCTGCAG 
GCCTGGCAGC GGCGCTTTGT GCAGCTGGCC CAGGAGGTCT TGCCTACAAA CGCATCCCAG 
CAGGTCCACG CCTTCTCCTC CACCACCCTG GACGACATCC TGCATGCCTT CTCTGAAGTC 
AGTGTTACGC GCGTGGTGGG AGGCTATCTG CTCATGCTGG CCTATGCCTG TGTGACGATG 
CTGAGGTGGG ACTGTGCCCA GTCTCAGGGT GCTGTGGGCC TTGCAGGGGT GCTGCTGGTT 
GCCCTGGCAG TAGCCTCTGG CCTTGGACTC TGTGCCTTGC TTGACATTGC CTTCAATGCT 
GCCACTACCC AGGTGCTGCC CTTCTTGGCA CTGGGCATCG GTGTAGATGA CATTTTCCTG 
CTGGCACATG CTTTCACAGA GGTGCCATCT GGCACCCCTC TCCAGGAGCA CACAGGCGCA 
TGTCTGCAGC GTACGGGCAC CAGCGTGGCA CTCACATCCA TCAACAACAT GGTTGCCTTC 
TTCATGGCTG CCCTGGTGCC CATCCCTGCA CTGCGGGCCT TCTCCTTGCA GGCAGCCATT 
GTGGTCAGCT GCACCTTCGC TGTTGTGATG CTTGTCTTCC CAGCAGTCCT CAGTCTAGAC 
CTGCACCGAC GCCACTCCCA GCGCCTGGAT GTACTCTGCT GCTTCTCCAG CCCCTGCTCT 
GCTCGGGTGA TTCAGATTCT ACCCCAGGAG CTGGGAGACA GGACGGTCCC AGTGGGCATC 
ACCCGCCTGA CAGCCACAGT TCAAGCCTTT GCCCACTGTG AAGCCAGCAG CCAGCATGTG 
GTCACCATTC TGCCTCCCCA AACCCACCTG GTGTCCCCAC CATCTGACCC ACTGGGCTCT 
GAGCTCTTCA TCCCAGGAGG CTCCACGCGG GACCTTCTAG GCCAGGAGGA GGGGACAAGG 
CAGAAGATAG CCCACAGGTC CCCACCCTGT GCCTGCTGGA ATCTTGCCCG TTTCGCCCGC 
TATCAGTTTG CACCGTTACT GCTTCAGTCA CATACCAAGG CTTTGGTGCT GGTGCTCTTT 
GGGGCTCTGC TTGGCCTGAG CCTCTATGGA GCCACACTGG TGCAGGATGG GCTGGCCCTG 
ACAGACGTGG TGCCTCGGGG CACCAAGGAA CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCCCTGT ATGAGGTGGC CCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGCTCCCTCA AGGCCGTGTT GCCCCCACCC 
ACCTCCCAGC CGCCCCGCAC CTGGCTGCAC TATTACCGCA ACTGGCTACA GGGAATCCAG 
GCTGCCTTTG ACCAGGACTG GGCTGCAGGC CGCATTACCC GCCACTCCTA CCGCAATGCC 
TCTGAGGATG GGGCGCTGGC CTATAAGCTG CTCATCCAGA CGGGGGACAC CCAGGAGCCA 
CTGGACTTCA GCCAGCTGCC CACAAGGAAG CTGGTAGACA CAGAGGGGCT GATCCCGCCA 
GAGCTTTTCT ACTCGGGGCT GACCGTGTGG GTGAACAGTG ATCCCCTGGG CCTGGCAGCC 
TCACAAGCCA ACTTCTACCC CCCACCTCCC GAGTGGCTAC ATGACAAATA CGACACCACC 
GGAGAGAACC TCCGCATCCC AGCGGCCCAG CCCCTCGAGT TTGCCCAGTT TCCCTTCCTG 
CTGCGTGGCC TCCAGAGTAC TGCAGACTTC GTGGAAGCCA TTGAGGGGGC CCGAGCAGCG 
TGTGCTGAGG CTGGCAGAGC TGGAGTGCGT GCCTACCCCA GTGGCTCCCC CTTCCTTTTC 
TGGGAGCAGT ATCTGGGCCT GCGGCGCTGC TTCCTGCTGG CTGTGGGCAT CCTGCTGGTC 
TGCACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCTT GGATGGCTGG CCTCATAGTG 
CTGGTCCTGG CGATGATGAC CATGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGCGCTA TCCCCGTGGT GATCCTCGTG GCCTCTGTGG GCATTGGTGT GGAGTTCACG 
GTCCACGTGG CTCTGGGATT TCTGACCACC CAAGGGAGCC GGAGCCTACG AGCTGCCCAT 
GCCCTGGAAC ACACATTTGC CCCGGTGACT GACGGGGCTG TCTCCACTCT GCTGGGTCTG 
CTCATGCTTG CTGGTTCCAG CTTCGACTTC ATCGTAAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016047139.1
CDS	33..2954
Translation	MLIQTPRQEGENVLTPEALGLHLQAALTASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAQELDGGCHGFSHKFMHWQEELLLGGMTRDPQGQLLRAEALQTTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAATVLQAWQRRFVQLAQEVLPTNASQQVHAFSSTTLDDILHAFSEVSVTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLDIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEVPSGTPLQEHTGACLQRTGTSVALTSINNMVAFFMAALVPIPALRAFSLQAAIVVSCTFAVVMLVFPAVLSLDLHRRHSQRLDVLCCFSSPCSARVIQILPQELGDRTVPVGITRLTATVQAFAHCEASSQHVVTILPPQTHLVSPPSDPLGSELFIPGGSTRDLLGQEEGTRQKIAHRSPPCACWNLARFARYQFAPLLLQSHTKALVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPTSQPPRTWLHYYRNWLQGIQAAFDQDWAAGRITRHSYRNASEDGALAYKLLIQTGDTQEPLDFSQLPTRKLVDTEGLIPPELFYSGLTVWVNSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQSTADFVEAIEGARAACAEAGRAGVRAYPSGSPFLFWEQYLGLRRCFLLAVGILLVCTFLVCALLLLNPWMAGLIVLVLAMMTMELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRSLRAAHALEHTFAPVTDGAVSTLLGLLMLAGSSFDFIVR

Target ORF information:

RefSeq Version	XM_016191653.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus patched 2 (PTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016191653.1

ORF Insert Sequence:

ATGTTGATAC AGACCCCACG ACAGGAGGGG GAGAATGTCC TCACACCTGA GGCTCTCGGC 
CTCCACCTCC AGGCAGCCCT CACTGCTAGT AAAGTGCAAG TATCACTCTA TGGAAAATCC 
TGGGATCTAA ACAAAATCTG CTACAAGTCA GGAGTGCCCC TCATTGAAAA CGGAATGATT 
GAGCGGATGA TTGAGAAGCT GTTTCCATGT GTGATCCTCA CCCCCCTCGA CTGCTTCTGG 
GAGGGAGCCA AACTCCAAGG GGGCTCCGCC TACTTACCGG GTCGGCCTGA CATCCAGTGG 
ACCAACCTGG ACCCGGAGCA GCTGCTAGAG GAGCTGGGCC CCTTCGCCTC CCTTGAGGGC 
TTCCGAGAGC TGCTCGACAA GGCACAGGTG GGCCAGGCCT ACGTGGGGAG GCCCTGTCTG 
CACCCGGATG ACCTTCACTG CCCTCCTAGC GCCCCTAATC ACCACAGCAG GCAGGCTCCC 
AATGTGGCTC AGGAGCTGGA TGGAGGCTGC CATGGCTTCT CTCACAAGTT CATGCATTGG 
CAGGAGGAGT TGCTGCTGGG AGGCATGACC AGAGACCCCC AGGGACAGCT ACTAAGGGCA 
GAGGCTCTGC AAACCACCTT CCTGCTGATG AGTCCCCGGC AGCTGTATGA GCATTTCCGG 
GGAGACTACC AGACACACGA CATCGGCTGG AGTGAGGAGC AGGCTGCCAC CGTGCTGCAG 
GCCTGGCAGC GGCGCTTTGT GCAGCTGGCC CAGGAGGTCT TGCCTACAAA CGCATCCCAG 
CAGGTCCACG CCTTCTCCTC CACCACCCTG GACGACATCC TGCATGCCTT CTCTGAAGTC 
AGTGTTACGC GCGTGGTGGG AGGCTATCTG CTCATGCTGG CCTATGCCTG TGTGACGATG 
CTGAGGTGGG ACTGTGCCCA GTCTCAGGGT GCTGTGGGCC TTGCAGGGGT GCTGCTGGTT 
GCCCTGGCAG TAGCCTCTGG CCTTGGACTC TGTGCCTTGC TTGACATTGC CTTCAATGCT 
GCCACTACCC AGGTGCTGCC CTTCTTGGCA CTGGGCATCG GTGTAGATGA CATTTTCCTG 
CTGGCACATG CTTTCACAGA GGTGCCATCT GGCACCCCTC TCCAGGAGCA CACAGGCGCA 
TGTCTGCAGC GTACGGGCAC CAGCGTGGCA CTCACATCCA TCAACAACAT GGTTGCCTTC 
TTCATGGCTG CCCTGGTGCC CATCCCTGCA CTGCGGGCCT TCTCCTTGCA GGCAGCCATT 
GTGGTCAGCT GCACCTTCGC TGTTGTGATG CTTGTCTTCC CAGCAGTCCT CAGTCTAGAC 
CTGCACCGAC GCCACTCCCA GCGCCTGGAT GTACTCTGCT GCTTCTCCAG CCCCTGCTCT 
GCTCGGGTGA TTCAGATTCT ACCCCAGGAG CTGGGAGACA GGACGGTCCC AGTGGGCATC 
ACCCGCCTGA CAGCCACAGT TCAAGCCTTT GCCCACTGTG AAGCCAGCAG CCAGCATGTG 
GTCACCATTC TGCCTCCCCA AACCCACCTG GTGTCCCCAC CATCTGACCC ACTGGGCTCT 
GAGCTCTTCA TCCCAGGAGG CTCCACGCGG GACCTTCTAG GCCAGGAGGA GGGGACAAGG 
CAGAAGATAG CCCACAGGTC CCCACCCTGT GCCTGCTGGA ATCTTGCCCG TTTCGCCCGC 
TATCAGTTTG CACCGTTACT GCTTCAGTCA CATACCAAGG CTTTGGTGCT GGTGCTCTTT 
GGGGCTCTGC TTGGCCTGAG CCTCTATGGA GCCACACTGG TGCAGGATGG GCTGGCCCTG 
ACAGACGTGG TGCCTCGGGG CACCAAGGAA CATGCCTTCC TGAGCGCCCA GCTCAGGTAC 
TTCTCCCTGT ATGAGGTGGC CCTGGTGACA CAGGGTGGCT TTGACTACGC CCACTCCCAA 
CGCGCCCTCT TTGATCTGCA CCAGCGCTTC AGCTCCCTCA AGGCCGTGTT GCCCCCACCC 
ACCTCCCAGC CGCCCCGCAC CTGGCTGCAC TATTACCGCA ACTGGCTACA GGGAATCCAG 
GCTGCCTTTG ACCAGGACTG GGCTGCAGGC CGCATTACCC GCCACTCCTA CCGCAATGCC 
TCTGAGGATG GGGCGCTGGC CTATAAGCTG CTCATCCAGA CGGGGGACAC CCAGGAGCCA 
CTGGACTTCA GCCAGCTGCC CACAAGGAAG CTGGTAGACA CAGAGGGGCT GATCCCGCCA 
GAGCTTTTCT ACTCGGGGCT GACCGTGTGG GTGAACAGTG ATCCCCTGGG CCTGGCAGCC 
TCACAAGCCA ACTTCTACCC CCCACCTCCC GAGTGGCTAC ATGACAAATA CGACACCACC 
GGAGAGAACC TCCGCATCCC AGCGGCCCAG CCCCTCGAGT TTGCCCAGTT TCCCTTCCTG 
CTGCGTGGCC TCCAGAGTAC TGCAGACTTC GTGGAAGCCA TTGAGGGGGC CCGAGCAGCG 
TGTGCTGAGG CTGGCAGAGC TGGAGTGCGT GCCTACCCCA GTGGCTCCCC CTTCCTTTTC 
TGGGAGCAGT ATCTGGGCCT GCGGCGCTGC TTCCTGCTGG CTGTGGGCAT CCTGCTGGTC 
TGCACTTTCC TCGTCTGTGC CCTGCTGCTG CTCAACCCTT GGATGGCTGG CCTCATAGTG 
CTGGTCCTGG CGATGATGAC CATGGAGCTC TTTGGCATCA TGGGTTTCCT GGGCATCAAG 
CTGAGCGCTA TCCCCGTGGT GATCCTCGTG GCCTCTGTGG GCATTGGTGT GGAGTTCACG 
GTCCACGTGG CTCTGGGATT TCTGACCACC CAAGGGAGCC GGAGCCTACG AGCTGCCCAT 
GCCCTGGAAC ACACATTTGC CCCGGTGACT GACGGGGCTG TCTCCACTCT GCTGGGTCTG 
CTCATGCTTG CTGGTTCCAG CTTCGACTTC ATCGTAAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_016191654.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016047140.1 Erinaceus europaeus patched 2 (PTCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEr19090
Clone ID Related Accession (Same CDS sequence)	XM_016191654.1
Accession Version	XM_016191654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804424.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGATC CTCACCCCCC TCGACTGCTT CTGGGAGGGA GCCAAACTCC AAGGGGGCTC 
CGCCTACTTA CCGCTCCCAA TGTGGCTCAG GAGCTGGATG GAGGCTGCCA TGGCTTCTCT 
CACAAGTTCA TGCATTGGCA GGAGGAGTTG CTGCTGGGAG GCATGACCAG AGACCCCCAG 
GGACAGCTAC TAAGGGCAGA GGCTCTGCAA ACCACCTTCC TGCTGATGAG TCCCCGGCAG 
CTGTATGAGC ATTTCCGGGG AGACTACCAG ACACACGACA TCGGCTGGAG TGAGGAGCAG 
GCTGCCACCG TGCTGCAGGC CTGGCAGCGG CGCTTTGTGC AGCTGGCCCA GGAGGTCTTG 
CCTACAAACG CATCCCAGCA GGTCCACGCC TTCTCCTCCA CCACCCTGGA CGACATCCTG 
CATGCCTTCT CTGAAGTCAG TGTTACGCGC GTGGTGGGAG GCTATCTGCT CATGCTGGCC 
TATGCCTGTG TGACGATGCT GAGGTGGGAC TGTGCCCAGT CTCAGGGTGC TGTGGGCCTT 
GCAGGGGTGC TGCTGGTTGC CCTGGCAGTA GCCTCTGGCC TTGGACTCTG TGCCTTGCTT 
GACATTGCCT TCAATGCTGC CACTACCCAG GTGCTGCCCT TCTTGGCACT GGGCATCGGT 
GTAGATGACA TTTTCCTGCT GGCACATGCT TTCACAGAGG TGCCATCTGG CACCCCTCTC 
CAGCACACAG GCGCATGTCT GCAGCGTACG GGCACCAGCG TGGCACTCAC ATCCATCAAC 
AACATGGTTG CCTTCTTCAT GGCTGCCCTG GTGCCCATCC CTGCACTGCG GGCCTTCTCC 
TTGCAGGCAG CCATTGTGGT CAGCTGCACC TTCGCTGTTG TGATGCTTGT CTTCCCAGCA 
GTCCTCAGTC TAGACCTGCA CCGACGCCAC TCCCAGCGCC TGGATGTACT CTGCTGCTTC 
TCCAGCCCCT GCTCTGCTCG GGTGATTCAG ATTCTACCCC AGGAGCTGGG AGACAGGACG 
GTCCCAGTGG GCATCACCCG CCTGACAGCC ACAGTTCAAG CCTTTGCCCA CTGTGAAGCC 
AGCAGCCAGC ATGTGGTCAC CATTCTGCCT CCCCAAACCC ACCTGGTGTC CCCACCATCT 
GACCCACTGG GCTCTGAGCT CTTCATCCCA GGAGGCTCCA CGCGGGACCT TCTAGGCCAG 
GAGGAGGGGA CAAGGCAGAA GATAGCCCAC AGGTCCCCAC CCTGTGCCTG CTGGAATCTT 
GCCCGTTTCG CCCGCTATCA GTTTGCACCG TTACTGCTTC AGTCACATAC CAAGGCTTTG 
GTGCTGGTGC TCTTTGGGGC TCTGCTTGGC CTGAGCCTCT ATGGAGCCAC ACTGGTGCAG 
GATGGGCTGG CCCTGACAGA CGTGGTGCCT CGGGGCACCA AGGAACATGC CTTCCTGAGC 
GCCCAGCTCA GGTACTTCTC CCTGTATGAG GTGGCCCTGG TGACACAGGG TGGCTTTGAC 
TACGCCCACT CCCAACGCGC CCTCTTTGAT CTGCACCAGC GCTTCAGCTC CCTCAAGGCC 
GTGTTGCCCC CACCCACCTC CCAGCCGCCC CGCACCTGGC TGCACTATTA CCGCAACTGG 
CTACAGGGAA TCCAGGCTGC CTTTGACCAG GACTGGGCTG CAGGCCGCAT TACCCGCCAC 
TCCTACCGCA ATGCCTCTGA GGATGGGGCG CTGGCCTATA AGCTGCTCAT CCAGACGGGG 
GACACCCAGG AGCCACTGGA CTTCAGCCAG CTGCCCACAA GGAAGCTGGT AGACACAGAG 
GGGCTGATCC CGCCAGAGCT TTTCTACTCG GGGCTGACCG TGTGGGTGAA CAGTGATCCC 
CTGGGCCTGG CAGCCTCACA AGCCAACTTC TACCCCCCAC CTCCCGAGTG GCTACATGAC 
AAATACGACA CCACCGGAGA GAACCTCCGC ATCCCAGCGG CCCAGCCCCT CGAGTTTGCC 
CAGTTTCCCT TCCTGCTGCG TGGCCTCCAG AGTACTGCAG ACTTCGTGGA AGCCATTGAG 
GGGGCCCGAG CAGCGTGTGC TGAGGCTGGC AGAGCTGGAG TGCGTGCCTA CCCCAGTGGC 
TCCCCCTTCC TTTTCTGGGA GCAGTATCTG GGCCTGCGGC GCTGCTTCCT GCTGGCTGTG 
GGCATCCTGC TGGTCTGCAC TTTCCTCGTC TGTGCCCTGC TGCTGCTCAA CCCTTGGATG 
GCTGGCCTCA TAGTGCTGGT CCTGGCGATG ATGACCATGG AGCTCTTTGG CATCATGGGT 
TTCCTGGGCA TCAAGCTGAG CGCTATCCCC GTGGTGATCC TCGTGGCCTC TGTGGGCATT 
GGTGTGGAGT TCACGGTCCA CGTGGCTCTG GGATTTCTGA CCACCCAAGG GAGCCGGAGC 
CTACGAGCTG CCCATGCCCT GGAACACACA TTTGCCCCGG TGACTGACGG GGCTGTCTCC 
ACTCTGCTGG GTCTGCTCAT GCTTGCTGGT TCCAGCTTCG ACTTCATCGT AAGGTACTTC 
TTCATGGTGC TGACCGTGCT CACAGTCCTG GGCCTCCTCC ACGGCCTCGT GCTGCTACCT 
GTGTTGCTGT CCATCCTGGG TCCCCCACCA GAGGTGGTGC AGGTGTACAA AGAAAACACA 
GAAGTGCTGA ACCCCCCAGC TCCACAGGGA GGAGGGCTCA GGTGGGGGGT TCCCCCCAGC 
CTGCATCCGA GTTTTGCGAG AGTGACTACC TCCATGACCG TGGCCCTCCA CCCACCCCCA 
CTACCTGGTG CCTATATCCA CCCAGCCTCG GATGAACCCG CTTGGCCACC TTCCACCACC 
CCAGCTGCCA GCAGCTCCAG CACCCCCATC TCTAGGGGAC CATGTCCAGC TACTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016047140.1
CDS	474..3353
Translation	MCDPHPPRLLLGGSQTPRGLRLLTAPNVAQELDGGCHGFSHKFMHWQEELLLGGMTRDPQGQLLRAEALQTTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAATVLQAWQRRFVQLAQEVLPTNASQQVHAFSSTTLDDILHAFSEVSVTRVVGGYLLMLAYACVTMLRWDCAQSQGAVGLAGVLLVALAVASGLGLCALLDIAFNAATTQVLPFLALGIGVDDIFLLAHAFTEVPSGTPLQHTGACLQRTGTSVALTSINNMVAFFMAALVPIPALRAFSLQAAIVVSCTFAVVMLVFPAVLSLDLHRRHSQRLDVLCCFSSPCSARVIQILPQELGDRTVPVGITRLTATVQAFAHCEASSQHVVTILPPQTHLVSPPSDPLGSELFIPGGSTRDLLGQEEGTRQKIAHRSPPCACWNLARFARYQFAPLLLQSHTKALVLVLFGALLGLSLYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVLPPPTSQPPRTWLHYYRNWLQGIQAAFDQDWAAGRITRHSYRNASEDGALAYKLLIQTGDTQEPLDFSQLPTRKLVDTEGLIPPELFYSGLTVWVNSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQFPFLLRGLQSTADFVEAIEGARAACAEAGRAGVRAYPSGSPFLFWEQYLGLRRCFLLAVGILLVCTFLVCALLLLNPWMAGLIVLVLAMMTMELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRSLRAAHALEHTFAPVTDGAVSTLLGLLMLAGSSFDFIVRYFFMVLTVLTVLGLLHGLVLLPVLLSILGPPPEVVQVYKENTEVLNPPAPQGGGLRWGVPPSLHPSFARVTTSMTVALHPPPLPGAYIHPASDEPAWPPSTTPAASSSSTPISRGPCPATG

Target ORF information:

RefSeq Version	XM_016191654.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus patched 2 (PTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016191654.1

ORF Insert Sequence:

ATGTGTGATC CTCACCCCCC TCGACTGCTT CTGGGAGGGA GCCAAACTCC AAGGGGGCTC 
CGCCTACTTA CCGCTCCCAA TGTGGCTCAG GAGCTGGATG GAGGCTGCCA TGGCTTCTCT 
CACAAGTTCA TGCATTGGCA GGAGGAGTTG CTGCTGGGAG GCATGACCAG AGACCCCCAG 
GGACAGCTAC TAAGGGCAGA GGCTCTGCAA ACCACCTTCC TGCTGATGAG TCCCCGGCAG 
CTGTATGAGC ATTTCCGGGG AGACTACCAG ACACACGACA TCGGCTGGAG TGAGGAGCAG 
GCTGCCACCG TGCTGCAGGC CTGGCAGCGG CGCTTTGTGC AGCTGGCCCA GGAGGTCTTG 
CCTACAAACG CATCCCAGCA GGTCCACGCC TTCTCCTCCA CCACCCTGGA CGACATCCTG 
CATGCCTTCT CTGAAGTCAG TGTTACGCGC GTGGTGGGAG GCTATCTGCT CATGCTGGCC 
TATGCCTGTG TGACGATGCT GAGGTGGGAC TGTGCCCAGT CTCAGGGTGC TGTGGGCCTT 
GCAGGGGTGC TGCTGGTTGC CCTGGCAGTA GCCTCTGGCC TTGGACTCTG TGCCTTGCTT 
GACATTGCCT TCAATGCTGC CACTACCCAG GTGCTGCCCT TCTTGGCACT GGGCATCGGT 
GTAGATGACA TTTTCCTGCT GGCACATGCT TTCACAGAGG TGCCATCTGG CACCCCTCTC 
CAGCACACAG GCGCATGTCT GCAGCGTACG GGCACCAGCG TGGCACTCAC ATCCATCAAC 
AACATGGTTG CCTTCTTCAT GGCTGCCCTG GTGCCCATCC CTGCACTGCG GGCCTTCTCC 
TTGCAGGCAG CCATTGTGGT CAGCTGCACC TTCGCTGTTG TGATGCTTGT CTTCCCAGCA 
GTCCTCAGTC TAGACCTGCA CCGACGCCAC TCCCAGCGCC TGGATGTACT CTGCTGCTTC 
TCCAGCCCCT GCTCTGCTCG GGTGATTCAG ATTCTACCCC AGGAGCTGGG AGACAGGACG 
GTCCCAGTGG GCATCACCCG CCTGACAGCC ACAGTTCAAG CCTTTGCCCA CTGTGAAGCC 
AGCAGCCAGC ATGTGGTCAC CATTCTGCCT CCCCAAACCC ACCTGGTGTC CCCACCATCT 
GACCCACTGG GCTCTGAGCT CTTCATCCCA GGAGGCTCCA CGCGGGACCT TCTAGGCCAG 
GAGGAGGGGA CAAGGCAGAA GATAGCCCAC AGGTCCCCAC CCTGTGCCTG CTGGAATCTT 
GCCCGTTTCG CCCGCTATCA GTTTGCACCG TTACTGCTTC AGTCACATAC CAAGGCTTTG 
GTGCTGGTGC TCTTTGGGGC TCTGCTTGGC CTGAGCCTCT ATGGAGCCAC ACTGGTGCAG 
GATGGGCTGG CCCTGACAGA CGTGGTGCCT CGGGGCACCA AGGAACATGC CTTCCTGAGC 
GCCCAGCTCA GGTACTTCTC CCTGTATGAG GTGGCCCTGG TGACACAGGG TGGCTTTGAC 
TACGCCCACT CCCAACGCGC CCTCTTTGAT CTGCACCAGC GCTTCAGCTC CCTCAAGGCC 
GTGTTGCCCC CACCCACCTC CCAGCCGCCC CGCACCTGGC TGCACTATTA CCGCAACTGG 
CTACAGGGAA TCCAGGCTGC CTTTGACCAG GACTGGGCTG CAGGCCGCAT TACCCGCCAC 
TCCTACCGCA ATGCCTCTGA GGATGGGGCG CTGGCCTATA AGCTGCTCAT CCAGACGGGG 
GACACCCAGG AGCCACTGGA CTTCAGCCAG CTGCCCACAA GGAAGCTGGT AGACACAGAG 
GGGCTGATCC CGCCAGAGCT TTTCTACTCG GGGCTGACCG TGTGGGTGAA CAGTGATCCC 
CTGGGCCTGG CAGCCTCACA AGCCAACTTC TACCCCCCAC CTCCCGAGTG GCTACATGAC 
AAATACGACA CCACCGGAGA GAACCTCCGC ATCCCAGCGG CCCAGCCCCT CGAGTTTGCC 
CAGTTTCCCT TCCTGCTGCG TGGCCTCCAG AGTACTGCAG ACTTCGTGGA AGCCATTGAG 
GGGGCCCGAG CAGCGTGTGC TGAGGCTGGC AGAGCTGGAG TGCGTGCCTA CCCCAGTGGC 
TCCCCCTTCC TTTTCTGGGA GCAGTATCTG GGCCTGCGGC GCTGCTTCCT GCTGGCTGTG 
GGCATCCTGC TGGTCTGCAC TTTCCTCGTC TGTGCCCTGC TGCTGCTCAA CCCTTGGATG 
GCTGGCCTCA TAGTGCTGGT CCTGGCGATG ATGACCATGG AGCTCTTTGG CATCATGGGT 
TTCCTGGGCA TCAAGCTGAG CGCTATCCCC GTGGTGATCC TCGTGGCCTC TGTGGGCATT 
GGTGTGGAGT TCACGGTCCA CGTGGCTCTG GGATTTCTGA CCACCCAAGG GAGCCGGAGC 
CTACGAGCTG CCCATGCCCT GGAACACACA TTTGCCCCGG TGACTGACGG GGCTGTCTCC 
ACTCTGCTGG GTCTGCTCAT GCTTGCTGGT TCCAGCTTCG ACTTCATCGT AAGGTACTTC 
TTCATGGTGC TGACCGTGCT CACAGTCCTG GGCCTCCTCC ACGGCCTCGT GCTGCTACCT 
GTGTTGCTGT CCATCCTGGG TCCCCCACCA GAGGTGGTGC AGGTGTACAA AGAAAACACA 
GAAGTGCTGA ACCCCCCAGC TCCACAGGGA GGAGGGCTCA GGTGGGGGGT TCCCCCCAGC 
CTGCATCCGA GTTTTGCGAG AGTGACTACC TCCATGACCG TGGCCCTCCA CCCACCCCCA 
CTACCTGGTG CCTATATCCA CCCAGCCTCG GATGAACCCG CTTGGCCACC TTCCACCACC 
CCAGCTGCCA GCAGCTCCAG CACCCCCATC TCTAGGGGAC CATGTCCAGC TACTGGGTGA