MAP3K3 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K3
Entrez Gene ID	103117577
Full Name	mitogen-activated protein kinase kinase kinase 3
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007527656.2	XP_007527718.2	mitogen-activated protein kinase kinase kinase 3

Pathways from BioSystems
Gene Ontology

The following MAP3K3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr15727	XM_007527656.2 Latest version!	Erinaceus europaeus mitogen-activated protein kinase kinase kinase 3 (MAP3K3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K3 ( XM_007527656.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007527718.2 Erinaceus europaeus mitogen-activated protein kinase kinase kinase 3 (MAP3K3), mRNA.

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CloneID	OEr15727
Clone ID Related Accession (Same CDS sequence)	XM_007527656.2
Accession Version	XM_007527656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	mitogen-activated protein kinase kinase kinase 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804267.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007527656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGAGAGTGG CGGTGCTGGT GCTAGCACAG CAGATAAAGC GTGGAGCTCT CAAACGGAGC 
ACCCAGAGTC CCGGCTGTGC AGCTGGTGAA GTGCTTGGCT GGCCTGTATT CAAGAGTTGC 
GTCTCTCTCG TTGCAGATGA GCAGCAGGCC CTGGACTCAA TCATGAAGGA CCTGGTGGCC 
CTCCAGATGA GCCGAAGGAC CCGGATGTCT GGATATGAGA CCGTGAAGAG CAAAGACACG 
GCTCACCCAA ACAGGCAGAG TGACGTCCGA ATCAAGTTTG AGCACAACGG GGAGAGGCGA 
ATTATAGCAT TTGGCCGACC TGTGAGGTAT GAAGATGTGG AACTTAAGGT GACAACGGTA 
TTTGGGCAGC CCCTTGATCT GCACTACATG AACAATGAGC TCTCCATCCT GCTGAAGAAC 
CAAGGTGATC TTGACCAAGC GGTCGGCATT CTGGACAGAA GCTCCAGCAT GAAGAGCCTG 
CGAATACTGC TACTGCCCCA GGACAGGAGC CAGCCCAGTT CCTCTCCCCA CTCTGGGGTG 
GCCCGGCAGG TGAGGATCAA AGCCTCCCAC TCAGCAGGAG ATGTAAACAC CGTCTACCAG 
CCCCCTGAGC CCAGAAGCAG GCATCTCTCT GTCAGCTCCC AGAACCCTGG CCGAAGCTCA 
CCTCCACCTG GGTATGTACC TGAGAGGCAG CAGCGGATTG CCCGGCAGGG ATCCTACACC 
AGCATCAACA GCGAGGGCGA GTTCATTCCA GAGACCAGTG AGCAGTGTAT GCTGGATCCT 
CTGAGCAGTG CTGAGAACTC CTTGTCAGGA AGCTGTCAGT CCTTGGACAG GTCAGCTGAC 
AGCCCCTCCT CCCGGAAGTC TCGAATGTCC AGAGCCCAGA GCTTCCCAGA TAACAGACAG 
GAGTTCTCAG ATCGGGACAC TCAGCTCTAT GACAAAGGGG TCAAAGGTGG CACCTACCCC 
CGGCGCTACC ATGTGTCTGT ACACCACAAG GACTACAGTG ATGGCCGAAG AACATTTCCC 
CGGATACGGC GTCATCAGGG CAACCTGTTC ACTCTGGTGC CCTCCAGTCG CTCGTTGAGC 
ACGAATGGTG AGAACGTGGG CCTAGCGGTG CAGTACCTGG ATGCCCGAGG ACGCCTGCGG 
AGTGCGGACA GCGACAACGC CCTCTCTGTG CAGGAGAGGA CTGTGCCGAC CAAGTCCCCG 
AGTGCCCCCA TCAACTGGCG CCGTGGCAAG CTCCTGGGCC AGGGGGCCTT TGGCAGGGTC 
TATTTGTGCT ATGATGTGGA CACGGGACGC GAACTCGCTT CCAAGCAGGT CCAGTTTGAC 
CCAGACAGTC CTGAAACAAG CAAGGAGGTG AGTGCTCTGG AGTGTGAGAT CCAGTTGCTG 
AAGAACCTGC AGCATGAGCG GATCGTGCAG TACTACGGCT GTCTGCGGGA CCGGGCAGAG 
AAGACCCTGA CCATCTTCAT GGAGTATATG CCAGGGGGCT CAGTGAAGGA CCAGCTGAAG 
GCCTACGGAG CTCTGACAGA AAGTGTGACC CGCAAGTACA CGCGGCAGAT CCTGGAGGGC 
GTGTCCTACC TGCACAGCAA CATGATCGTG CACCGTGACA TCAAAGGTGC CAACATCCTG 
CGAGACTCCG CTGGAAACGT GAAGCTGGGA GACTTCGGGG CCAGCAAGCG GCTGCAGACC 
ATCTGCATGT CGGGCACGGG CATGCGCTCC GTCACGGGCA CTCCCTACTG GATGAGCCCC 
GAGGTGATCA GCGGCGAGGG CTACGGAAGG AAGGCAGACG TGTGGAGCCT GGGGTGCACC 
GTCGTGGAGA TGCTGACGGA GAAGCCGCCG TGGGCAGAGT ACGAGGCCAT GGCCGCCATC 
TTCAAGATCG CCACGCAGCC CACCAACCCG CAGCTGCCCT CCCACATCTC TGAGCACGGC 
CGGGACTTCC TGCGGCGCAT CTTCGTGGAG GCCCGCCGGA GGCCTTCAGC CGAGGAGCTG 
CTCACACACC ACTTTGCACA GCTCGTGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007527718.2
CDS	1..2013
Translation	MRVAVLVLAQQIKRGALKRSTQSPGCAAGEVLGWPVFKSCVSLVADEQQALDSIMKDLVALQMSRRTRMSGYETVKSKDTAHPNRQSDVRIKFEHNGERRIIAFGRPVRYEDVELKVTTVFGQPLDLHYMNNELSILLKNQGDLDQAVGILDRSSSMKSLRILLLPQDRSQPSSSPHSGVARQVRIKASHSAGDVNTVYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQRIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSSRKSRMSRAQSFPDNRQEFSDRDTQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENVGLAVQYLDARGRLRSADSDNALSVQERTVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGVSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARRRPSAEELLTHHFAQLVY

Target ORF information:

RefSeq Version	XM_007527656.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus mitogen-activated protein kinase kinase kinase 3 (MAP3K3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007527656.2

ORF Insert Sequence:

ATGAGAGTGG CGGTGCTGGT GCTAGCACAG CAGATAAAGC GTGGAGCTCT CAAACGGAGC 
ACCCAGAGTC CCGGCTGTGC AGCTGGTGAA GTGCTTGGCT GGCCTGTATT CAAGAGTTGC 
GTCTCTCTCG TTGCAGATGA GCAGCAGGCC CTGGACTCAA TCATGAAGGA CCTGGTGGCC 
CTCCAGATGA GCCGAAGGAC CCGGATGTCT GGATATGAGA CCGTGAAGAG CAAAGACACG 
GCTCACCCAA ACAGGCAGAG TGACGTCCGA ATCAAGTTTG AGCACAACGG GGAGAGGCGA 
ATTATAGCAT TTGGCCGACC TGTGAGGTAT GAAGATGTGG AACTTAAGGT GACAACGGTA 
TTTGGGCAGC CCCTTGATCT GCACTACATG AACAATGAGC TCTCCATCCT GCTGAAGAAC 
CAAGGTGATC TTGACCAAGC GGTCGGCATT CTGGACAGAA GCTCCAGCAT GAAGAGCCTG 
CGAATACTGC TACTGCCCCA GGACAGGAGC CAGCCCAGTT CCTCTCCCCA CTCTGGGGTG 
GCCCGGCAGG TGAGGATCAA AGCCTCCCAC TCAGCAGGAG ATGTAAACAC CGTCTACCAG 
CCCCCTGAGC CCAGAAGCAG GCATCTCTCT GTCAGCTCCC AGAACCCTGG CCGAAGCTCA 
CCTCCACCTG GGTATGTACC TGAGAGGCAG CAGCGGATTG CCCGGCAGGG ATCCTACACC 
AGCATCAACA GCGAGGGCGA GTTCATTCCA GAGACCAGTG AGCAGTGTAT GCTGGATCCT 
CTGAGCAGTG CTGAGAACTC CTTGTCAGGA AGCTGTCAGT CCTTGGACAG GTCAGCTGAC 
AGCCCCTCCT CCCGGAAGTC TCGAATGTCC AGAGCCCAGA GCTTCCCAGA TAACAGACAG 
GAGTTCTCAG ATCGGGACAC TCAGCTCTAT GACAAAGGGG TCAAAGGTGG CACCTACCCC 
CGGCGCTACC ATGTGTCTGT ACACCACAAG GACTACAGTG ATGGCCGAAG AACATTTCCC 
CGGATACGGC GTCATCAGGG CAACCTGTTC ACTCTGGTGC CCTCCAGTCG CTCGTTGAGC 
ACGAATGGTG AGAACGTGGG CCTAGCGGTG CAGTACCTGG ATGCCCGAGG ACGCCTGCGG 
AGTGCGGACA GCGACAACGC CCTCTCTGTG CAGGAGAGGA CTGTGCCGAC CAAGTCCCCG 
AGTGCCCCCA TCAACTGGCG CCGTGGCAAG CTCCTGGGCC AGGGGGCCTT TGGCAGGGTC 
TATTTGTGCT ATGATGTGGA CACGGGACGC GAACTCGCTT CCAAGCAGGT CCAGTTTGAC 
CCAGACAGTC CTGAAACAAG CAAGGAGGTG AGTGCTCTGG AGTGTGAGAT CCAGTTGCTG 
AAGAACCTGC AGCATGAGCG GATCGTGCAG TACTACGGCT GTCTGCGGGA CCGGGCAGAG 
AAGACCCTGA CCATCTTCAT GGAGTATATG CCAGGGGGCT CAGTGAAGGA CCAGCTGAAG 
GCCTACGGAG CTCTGACAGA AAGTGTGACC CGCAAGTACA CGCGGCAGAT CCTGGAGGGC 
GTGTCCTACC TGCACAGCAA CATGATCGTG CACCGTGACA TCAAAGGTGC CAACATCCTG 
CGAGACTCCG CTGGAAACGT GAAGCTGGGA GACTTCGGGG CCAGCAAGCG GCTGCAGACC 
ATCTGCATGT CGGGCACGGG CATGCGCTCC GTCACGGGCA CTCCCTACTG GATGAGCCCC 
GAGGTGATCA GCGGCGAGGG CTACGGAAGG AAGGCAGACG TGTGGAGCCT GGGGTGCACC 
GTCGTGGAGA TGCTGACGGA GAAGCCGCCG TGGGCAGAGT ACGAGGCCAT GGCCGCCATC 
TTCAAGATCG CCACGCAGCC CACCAACCCG CAGCTGCCCT CCCACATCTC TGAGCACGGC 
CGGGACTTCC TGCGGCGCAT CTTCGTGGAG GCCCGCCGGA GGCCTTCAGC CGAGGAGCTG 
CTCACACACC ACTTTGCACA GCTCGTGTAC TGA