PPP1R12A cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R12A
Entrez Gene ID	103115804
Full Name	protein phosphatase 1 regulatory subunit 12A
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007525652.2	XP_007525714.1	protein phosphatase 1 regulatory subunit 12A isoform X3
XM_007525650.2	XP_007525712.1	protein phosphatase 1 regulatory subunit 12A isoform X1
XM_007525651.2	XP_007525713.1	protein phosphatase 1 regulatory subunit 12A isoform X2

Pathways from BioSystems
Gene Ontology

The following PPP1R12A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R12A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OEr13674	XM_007525651.2 Latest version!	Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr13675	XM_007525652.2 Latest version!	Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr13673	XM_007525650.2 Latest version!	Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R12A ( XM_007525651.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007525713.1 Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEr13674
Clone ID Related Accession (Same CDS sequence)	XM_007525651.2
Accession Version	XM_007525651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	protein phosphatase 1 regulatory subunit 12A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804197.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007525651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TCAAGCGCTG GATCGGCTCC 
GAGACGGACC TGGAGCCGCC GGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGACGAT 
GGCGCCGTCT TCCTGGCCGC CTGCTCCAGC GGCGACACGG ACGAGGTGCT CCGCCTGCTG 
CACCGCGGCG CCGACATCAA CTACGCCAAT GTGGACGGGC TCACCGCCCT GCACCAGGCT 
TGCATTGATG ACAATGTGGA AATGGTGAAG TTTCTGGTGG AAAATGGAGC AAATATTAAT 
CAACCTGATA ACGAAGGCTG GATACCACTG CATGCAGCTG CTTCTTGTGG ATATCTTGAT 
ATTGCAGAGT TTTTAATCGG CCAAGGAGCA CATGTAGGCG CTGTCAACAG TGAAGGGGAC 
ACTCCTTTAG ATATTGCTGA GGAGGAGGCG ATGGAAGAAC TACTGCAAAA TGAAGTGAAC 
CGACAAGGAG TTGACATAGA AGCAGCTCGA AAGGAAGAAG AGCGGATCAT GCTGAGAGAC 
GCCAGGCAGT GGCTGAACAG CGGTCACATC AGTGACGTCC GGCACGCCAA GTCCGGAGGC 
ACAGCGCTCC ACGTGGCTGC TGCTAAAGGC TACACAGAAG TTTTAAAACT TTTAATACAG 
GCAGGCTATG ATGTTAACGT CAAGGACTAT GATGGCTGGA CTCCTCTTCA CGCCGCGGCT 
CACTGGGGTA AAGAAGAAGC TTGTCGCATC TTAGTGGACA ATCTGTGTGA CATGGAGATG 
GTCAACAAAG TGGGCCAAAC AGCCTTTGAC GTAGCAGATG AAGACATTTT AGGCTATTTA 
GAAGAGTTGC AAAAGAAGCA GAATCTGCTG CATAGTGAAA AACGGGAGAA GAAATCTCCT 
CTAATTGAGT CAACAGCCAA TATAGACAAT AATCAGTCAC AGAAGACCTT TAAAAACAAA 
GAGACACTGA TTATTGAGAC AGAGAAAAAT GCATCCCGTA TTGACTCTCT AGAACAAGAA 
AAGGTTGATG ATGAGGAAGA AGGAAAGAAA GATGAGTCTA GCTGCTCTAG CGAAGAAGAC 
GAAGAAGATG ACTCCGAGTC AGAAGCGGAA ACAGATAAGA CAAGACCCGT GGCTTCTGTA 
ACTAATGTCA GTACTGCAAG TACACCAGCA GCTCCTGTAG CTGTGACACC TACTGTCTCG 
TCTGGCCAGG CAACTCCCAC GTCACCTGTT AAAAAGTTTC CGACCTCCAC TACTAAAATT 
TCTCCCAAAG AAGAAGAGAG GAAAGATGAG TCTCCTGCAT CATGGAGGTT AGGACTCAGA 
AAGACTGGCA GTTACGGCGC ACTGGCCGAA ATCACAGCAT CTAAAGAGGC ACAGAAAGAA 
AAAGACATGA CAGGTGTCAT GCGTTCTGCA TCAAGCCCCA GGCTTTCCTC CTCTTTGGAT 
AACAAAGAAA AGGAAAAAGA TAGCAAAGGA ACTCGCCTCG CCTATGTCGC CCCTACAATA 
CCAAGACGGC TGGCCAGCAC GTCTGACATT GAAGAAAAAG AAAACAGAGA CTCTTTAAGT 
TTGCGAACAA GTAGTTCTTA CACAAGAAGG AAATGGGAAG ATGACCTCAA AAAGAATAGT 
TCAGTCAATG AAGGCTCAAC TTACCACAGA AGTTACTCCT TTGGTAGAAG ACAAGATGAT 
TTGATTAGTT CTAGTGTTCC AAGCACCACA TCAACACCAA CAGTTACCTC TGCAGCTGGG 
CTTCAGAAAA GCCTGCTCCC CAGCTCAAGC ACTCCTACAA AGATTGCAGT GGGCTCTTCC 
TCAGCAGGCA CACAAAGCAG TACCTCAAAT CGTTTGTGGG CTGAGGATAG TACTGAGAAG 
GAAAAGGACA GTGTCCCCAC GGCAGTGACC ATTCCCGTTG CTCCAACTGT TGTAAACGCT 
GCGGCCTCTA CCACAACCCT GACTACTACT ACTGCCGGCA CTGTCTCCTC TACGTCAGAG 
GTCAGGGAGA GACGCAGATC ATACCTCACT CCTGTTAGAG ATGAAGAGTC TGAATCCCAA 
AGAAAAGCAA GATCCAGGCA AGCAAGACAG TCGAGAAGAT CGACACAGGG AGTGACATTA 
ACTGATCTTC AGGAAGCGGA AAAAACAATA GGACGAAGTC GTTCTATCAG AACCAGAGAA 
CCAGAAAATG AAGAAAAAGA AAAAGAGGAA AAAGAAAAGC AGGATAAAGA GAAGCAGGAA 
GAAAAGAAGG AGTCAGAATT ATCTAGAGAA GATGAATTAA AACAGAAATA CTCCAGAACG 
TATGATGAGA CCTGCCAGCG TTACAGGCCA GTGTCGACTT CCAGTGTGAC CACTCCATCC 
TCTTCGCTTT CCACCATGAG CAGCTCTCTC TACACATCGA GTCAGCTAAG CAGACCAAAC 
AGTCTCATAG GGATAACTTC TGGCTACTCC AGAGGCTTAA CAAAAGAAAA TGAACGAGAG 
GGGGAGAAGA AAGAAGAGGA AAAAGAAGGA GAAGACAAAT CCCAGTCAAA ATCAGTCAGA 
GAACGACGGA GGCCAAGAGA GAAAAGAAGA TCTACAGGAG TTTCATTCTG GACACAAGAT 
AGTGATGAAA ATGAACAAGA ACAGCAGTCA GATACAGAAG AAGGATCCAA TAAGAAAGAA 
ACTCAGACGG ATTCCATTTC TAGATATGAA ACCAGTTCCA CTTCGACTGG TGAGCGGTCT 
GATTCCTCGC TGGGCCGCTC AGGATCATAC AGTTACTTAG AGGAGAGAAA GCCTTACAGC 
AGCAGACTAG AGAAGGACGT GTCGACTGAC TTTAAAAAGA GACAAGAAAG GTTTGCTGAT 
AGGTCACTAT TGGAAATGGA AAAAAGGGAA CGAAGAGCAC TAGAAAGAAG AATATCTGAA 
ATGGAAGAAG AGCTCAAAAT GTTGCCAGAC CTAAAAGCAG ACAACCAGAG GCTAAAGGAT 
GAAAATGGGG CCTTGATCAG AGTTATAAGC AAACTTTCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007525713.1
CDS	129..3113
Translation	MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLRLLHRGADINYANVDGLTALHQACIDDNVEMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNVKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKREKKSPLIESTANIDNNQSQKTFKNKETLIIETEKNASRIDSLEQEKVDDEEEGKKDESSCSSEEDEEDDSESEAETDKTRPVASVTNVSTASTPAAPVAVTPTVSSGQATPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDMTGVMRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPTIPRRLASTSDIEEKENRDSLSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHRSYSFGRRQDDLISSSVPSTTSTPTVTSAAGLQKSLLPSSSTPTKIAVGSSSAGTQSSTSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSIRTREPENEEKEKEEKEKQDKEKQEEKKESELSREDELKQKYSRTYDETCQRYRPVSTSSVTTPSSSLSTMSSSLYTSSQLSRPNSLIGITSGYSRGLTKENEREGEKKEEEKEGEDKSQSKSVRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSTGERSDSSLGRSGSYSYLEERKPYSSRLEKDVSTDFKKRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK

Target ORF information:

RefSeq Version	XM_007525651.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007525651.2

ORF Insert Sequence:

ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TCAAGCGCTG GATCGGCTCC 
GAGACGGACC TGGAGCCGCC GGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGACGAT 
GGCGCCGTCT TCCTGGCCGC CTGCTCCAGC GGCGACACGG ACGAGGTGCT CCGCCTGCTG 
CACCGCGGCG CCGACATCAA CTACGCCAAT GTGGACGGGC TCACCGCCCT GCACCAGGCT 
TGCATTGATG ACAATGTGGA AATGGTGAAG TTTCTGGTGG AAAATGGAGC AAATATTAAT 
CAACCTGATA ACGAAGGCTG GATACCACTG CATGCAGCTG CTTCTTGTGG ATATCTTGAT 
ATTGCAGAGT TTTTAATCGG CCAAGGAGCA CATGTAGGCG CTGTCAACAG TGAAGGGGAC 
ACTCCTTTAG ATATTGCTGA GGAGGAGGCG ATGGAAGAAC TACTGCAAAA TGAAGTGAAC 
CGACAAGGAG TTGACATAGA AGCAGCTCGA AAGGAAGAAG AGCGGATCAT GCTGAGAGAC 
GCCAGGCAGT GGCTGAACAG CGGTCACATC AGTGACGTCC GGCACGCCAA GTCCGGAGGC 
ACAGCGCTCC ACGTGGCTGC TGCTAAAGGC TACACAGAAG TTTTAAAACT TTTAATACAG 
GCAGGCTATG ATGTTAACGT CAAGGACTAT GATGGCTGGA CTCCTCTTCA CGCCGCGGCT 
CACTGGGGTA AAGAAGAAGC TTGTCGCATC TTAGTGGACA ATCTGTGTGA CATGGAGATG 
GTCAACAAAG TGGGCCAAAC AGCCTTTGAC GTAGCAGATG AAGACATTTT AGGCTATTTA 
GAAGAGTTGC AAAAGAAGCA GAATCTGCTG CATAGTGAAA AACGGGAGAA GAAATCTCCT 
CTAATTGAGT CAACAGCCAA TATAGACAAT AATCAGTCAC AGAAGACCTT TAAAAACAAA 
GAGACACTGA TTATTGAGAC AGAGAAAAAT GCATCCCGTA TTGACTCTCT AGAACAAGAA 
AAGGTTGATG ATGAGGAAGA AGGAAAGAAA GATGAGTCTA GCTGCTCTAG CGAAGAAGAC 
GAAGAAGATG ACTCCGAGTC AGAAGCGGAA ACAGATAAGA CAAGACCCGT GGCTTCTGTA 
ACTAATGTCA GTACTGCAAG TACACCAGCA GCTCCTGTAG CTGTGACACC TACTGTCTCG 
TCTGGCCAGG CAACTCCCAC GTCACCTGTT AAAAAGTTTC CGACCTCCAC TACTAAAATT 
TCTCCCAAAG AAGAAGAGAG GAAAGATGAG TCTCCTGCAT CATGGAGGTT AGGACTCAGA 
AAGACTGGCA GTTACGGCGC ACTGGCCGAA ATCACAGCAT CTAAAGAGGC ACAGAAAGAA 
AAAGACATGA CAGGTGTCAT GCGTTCTGCA TCAAGCCCCA GGCTTTCCTC CTCTTTGGAT 
AACAAAGAAA AGGAAAAAGA TAGCAAAGGA ACTCGCCTCG CCTATGTCGC CCCTACAATA 
CCAAGACGGC TGGCCAGCAC GTCTGACATT GAAGAAAAAG AAAACAGAGA CTCTTTAAGT 
TTGCGAACAA GTAGTTCTTA CACAAGAAGG AAATGGGAAG ATGACCTCAA AAAGAATAGT 
TCAGTCAATG AAGGCTCAAC TTACCACAGA AGTTACTCCT TTGGTAGAAG ACAAGATGAT 
TTGATTAGTT CTAGTGTTCC AAGCACCACA TCAACACCAA CAGTTACCTC TGCAGCTGGG 
CTTCAGAAAA GCCTGCTCCC CAGCTCAAGC ACTCCTACAA AGATTGCAGT GGGCTCTTCC 
TCAGCAGGCA CACAAAGCAG TACCTCAAAT CGTTTGTGGG CTGAGGATAG TACTGAGAAG 
GAAAAGGACA GTGTCCCCAC GGCAGTGACC ATTCCCGTTG CTCCAACTGT TGTAAACGCT 
GCGGCCTCTA CCACAACCCT GACTACTACT ACTGCCGGCA CTGTCTCCTC TACGTCAGAG 
GTCAGGGAGA GACGCAGATC ATACCTCACT CCTGTTAGAG ATGAAGAGTC TGAATCCCAA 
AGAAAAGCAA GATCCAGGCA AGCAAGACAG TCGAGAAGAT CGACACAGGG AGTGACATTA 
ACTGATCTTC AGGAAGCGGA AAAAACAATA GGACGAAGTC GTTCTATCAG AACCAGAGAA 
CCAGAAAATG AAGAAAAAGA AAAAGAGGAA AAAGAAAAGC AGGATAAAGA GAAGCAGGAA 
GAAAAGAAGG AGTCAGAATT ATCTAGAGAA GATGAATTAA AACAGAAATA CTCCAGAACG 
TATGATGAGA CCTGCCAGCG TTACAGGCCA GTGTCGACTT CCAGTGTGAC CACTCCATCC 
TCTTCGCTTT CCACCATGAG CAGCTCTCTC TACACATCGA GTCAGCTAAG CAGACCAAAC 
AGTCTCATAG GGATAACTTC TGGCTACTCC AGAGGCTTAA CAAAAGAAAA TGAACGAGAG 
GGGGAGAAGA AAGAAGAGGA AAAAGAAGGA GAAGACAAAT CCCAGTCAAA ATCAGTCAGA 
GAACGACGGA GGCCAAGAGA GAAAAGAAGA TCTACAGGAG TTTCATTCTG GACACAAGAT 
AGTGATGAAA ATGAACAAGA ACAGCAGTCA GATACAGAAG AAGGATCCAA TAAGAAAGAA 
ACTCAGACGG ATTCCATTTC TAGATATGAA ACCAGTTCCA CTTCGACTGG TGAGCGGTCT 
GATTCCTCGC TGGGCCGCTC AGGATCATAC AGTTACTTAG AGGAGAGAAA GCCTTACAGC 
AGCAGACTAG AGAAGGACGT GTCGACTGAC TTTAAAAAGA GACAAGAAAG GTTTGCTGAT 
AGGTCACTAT TGGAAATGGA AAAAAGGGAA CGAAGAGCAC TAGAAAGAAG AATATCTGAA 
ATGGAAGAAG AGCTCAAAAT GTTGCCAGAC CTAAAAGCAG ACAACCAGAG GCTAAAGGAT 
GAAAATGGGG CCTTGATCAG AGTTATAAGC AAACTTTCCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R12A ( XM_007525652.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007525714.1 Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OEr13675
Clone ID Related Accession (Same CDS sequence)	XM_007525652.2
Accession Version	XM_007525652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	protein phosphatase 1 regulatory subunit 12A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804197.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007525652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TCAAGCGCTG GATCGGCTCC 
GAGACGGACC TGGAGCCGCC GGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGACGAT 
GGCGCCGTCT TCCTGGCCGC CTGCTCCAGC GGCGACACGG ACGAGGTGCT CCGCCTGCTG 
CACCGCGGCG CCGACATCAA CTACGCCAAT GTGGACGGGC TCACCGCCCT GCACCAGGCT 
TGCATTGATG ACAATGTGGA AATGGTGAAG TTTCTGGTGG AAAATGGAGC AAATATTAAT 
CAACCTGATA ACGAAGGCTG GATACCACTG CATGCAGCTG CTTCTTGTGG ATATCTTGAT 
ATTGCAGAGT TTTTAATCGG CCAAGGAGCA CATGTAGGCG CTGTCAACAG TGAAGGGGAC 
ACTCCTTTAG ATATTGCTGA GGAGGAGGCG ATGGAAGAAC TACTGCAAAA TGAAGTGAAC 
CGACAAGGAG TTGACATAGA AGCAGCTCGA AAGGAAGAAG AGCGGATCAT GCTGAGAGAC 
GCCAGGCAGT GGCTGAACAG CGGTCACATC AGTGACGTCC GGCACGCCAA GTCCGGAGGC 
ACAGCGCTCC ACGTGGCTGC TGCTAAAGGC TACACAGAAG TTTTAAAACT TTTAATACAG 
GCAGGCTATG ATGTTAACGT CAAGGACTAT GATGGCTGGA CTCCTCTTCA CGCCGCGGCT 
CACTGGGGTA AAGAAGAAGC TTGTCGCATC TTAGTGGACA ATCTGTGTGA CATGGAGATG 
GTCAACAAAG TGGGCCAAAC AGCCTTTGAC GTAGCAGATG AAGACATTTT AGGCTATTTA 
GAAGAGTTGC AAAAGAAGCA GAATCTGCTG CATAGTGAAA AACGGGAGAA GAAATCTCCT 
CTAATTGAGT CAACAGCCAA TATAGACAAT AATCAGTCAC AGAAGACCTT TAAAAACAAA 
GAGACACTGA TTATTGAGAC AGAGAAAAAT GCATCCCGTA TTGACTCTCT AGAACAAGAA 
AAGGTTGATG ATGAGGAAGA AGGAAAGAAA GATGAGTCTA GCTGCTCTAG CGAAGAAGAC 
GAAGAAGATG ACTCCGAGTC AGAAGCGGAA ACAGATAAGA CAAGACCCGT GGCTTCTGTA 
ACTAATGTCA GTACTGCAAG TACACCAGCA GCTCCTGTAG CTGTGACACC TACTGTCTCG 
TCTGGCCAGG CAACTCCCAC GTCACCTGTT AAAAAGTTTC CGACCTCCAC TACTAAAATT 
TCTCCCAAAG AAGAAGAGAG GAAAGATGAG TCTCCTGCAT CATGGAGGTT AGGACTCAGA 
AAGACTGGCA GTTACGGCGC ACTGGCCGAA ATCACAGCAT CTAAAGAGGC ACAGAAAGAA 
AAAGACATGA CAGGTGTCAT GCGTTCTGCA TCAAGCCCCA GGCTTTCCTC CTCTTTGGAT 
AACAAAGAAA AGGAAAAAGA TAGCAAAGGA ACTCGCCTCG CCTATGTCGC CCCTACAATA 
CCAAGACGGC TGGCCAGCAC GTCTGACATT GAAGAAAAAG AAAACAGAGA CTCTTTAAGT 
TTGCGAACAA GTAGTTCTTA CACAAGAAGG AAATGGGAAG ATGACCTCAA AAAGAATAGT 
TCAGTCAATG AAGGCTCAAC TTACCACAGA AGTACCTCAA ATCGTTTGTG GGCTGAGGAT 
AGTACTGAGA AGGAAAAGGA CAGTGTCCCC ACGGCAGTGA CCATTCCCGT TGCTCCAACT 
GTTGTAAACG CTGCGGCCTC TACCACAACC CTGACTACTA CTACTGCCGG CACTGTCTCC 
TCTACGTCAG AGGTCAGGGA GAGACGCAGA TCATACCTCA CTCCTGTTAG AGATGAAGAG 
TCTGAATCCC AAAGAAAAGC AAGATCCAGG CAAGCAAGAC AGTCGAGAAG ATCGACACAG 
GGAGTGACAT TAACTGATCT TCAGGAAGCG GAAAAAACAA TAGGACGAAG TCGTTCTATC 
AGAACCAGAG AACCAGAAAA TGAAGAAAAA GAAAAAGAGG AAAAAGAAAA GCAGGATAAA 
GAGAAGCAGG AAGAAAAGAA GGAGTCAGAA TTATCTAGAG AAGATGAATT AAAACAGAAA 
TACTCCAGAA CGTATGATGA GACCTGCCAG CGTTACAGGC CAGTGTCGAC TTCCAGTGTG 
ACCACTCCAT CCTCTTCGCT TTCCACCATG AGCAGCTCTC TCTACACATC GAGTCAGCTA 
AGCAGACCAA ACAGTCTCAT AGGGATAACT TCTGGCTACT CCAGAGGCTT AACAAAAGAA 
AATGAACGAG AGGGGGAGAA GAAAGAAGAG GAAAAAGAAG GAGAAGACAA ATCCCAGTCA 
AAATCAGTCA GAGAACGACG GAGGCCAAGA GAGAAAAGAA GATCTACAGG AGTTTCATTC 
TGGACACAAG ATAGTGATGA AAATGAACAA GAACAGCAGT CAGATACAGA AGAAGGATCC 
AATAAGAAAG AAACTCAGAC GGATTCCATT TCTAGATATG AAACCAGTTC CACTTCGACT 
GGTGAGCGGT CTGATTCCTC GCTGGGCCGC TCAGGATCAT ACAGTTACTT AGAGGAGAGA 
AAGCCTTACA GCAGCAGACT AGAGAAGGAC GTGTCGACTG ACTTTAAAAA GCTTTATGAA 
AAAATCCTAG CTGAAAATGA AAAGCTAAAA GTTCAGCTGC ATGACACAAA CATGGAACTG 
ACAGATCTTA AATTGCAGTT GGAAAAAGCC ACCCAGAGAC AAGAAAGGTT TGCTGATAGG 
TCACTATTGG AAATGGAAAA AAGGGAACGA AGAGCACTAG AAAGAAGAAT ATCTGAAATG 
GAAGAAGAGC TCAAAATGTT GCCAGACCTA AAAGCAGACA ACCAGAGGCT AAAGGATGAA 
AATGGGGCCT TGATCAGAGT TATAAGCAAA CTTTCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007525714.1
CDS	129..3050
Translation	MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLRLLHRGADINYANVDGLTALHQACIDDNVEMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNVKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKREKKSPLIESTANIDNNQSQKTFKNKETLIIETEKNASRIDSLEQEKVDDEEEGKKDESSCSSEEDEEDDSESEAETDKTRPVASVTNVSTASTPAAPVAVTPTVSSGQATPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDMTGVMRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPTIPRRLASTSDIEEKENRDSLSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHRSTSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSIRTREPENEEKEKEEKEKQDKEKQEEKKESELSREDELKQKYSRTYDETCQRYRPVSTSSVTTPSSSLSTMSSSLYTSSQLSRPNSLIGITSGYSRGLTKENEREGEKKEEEKEGEDKSQSKSVRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSTGERSDSSLGRSGSYSYLEERKPYSSRLEKDVSTDFKKLYEKILAENEKLKVQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK

Target ORF information:

RefSeq Version	XM_007525652.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007525652.2

ORF Insert Sequence:

ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TCAAGCGCTG GATCGGCTCC 
GAGACGGACC TGGAGCCGCC GGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGACGAT 
GGCGCCGTCT TCCTGGCCGC CTGCTCCAGC GGCGACACGG ACGAGGTGCT CCGCCTGCTG 
CACCGCGGCG CCGACATCAA CTACGCCAAT GTGGACGGGC TCACCGCCCT GCACCAGGCT 
TGCATTGATG ACAATGTGGA AATGGTGAAG TTTCTGGTGG AAAATGGAGC AAATATTAAT 
CAACCTGATA ACGAAGGCTG GATACCACTG CATGCAGCTG CTTCTTGTGG ATATCTTGAT 
ATTGCAGAGT TTTTAATCGG CCAAGGAGCA CATGTAGGCG CTGTCAACAG TGAAGGGGAC 
ACTCCTTTAG ATATTGCTGA GGAGGAGGCG ATGGAAGAAC TACTGCAAAA TGAAGTGAAC 
CGACAAGGAG TTGACATAGA AGCAGCTCGA AAGGAAGAAG AGCGGATCAT GCTGAGAGAC 
GCCAGGCAGT GGCTGAACAG CGGTCACATC AGTGACGTCC GGCACGCCAA GTCCGGAGGC 
ACAGCGCTCC ACGTGGCTGC TGCTAAAGGC TACACAGAAG TTTTAAAACT TTTAATACAG 
GCAGGCTATG ATGTTAACGT CAAGGACTAT GATGGCTGGA CTCCTCTTCA CGCCGCGGCT 
CACTGGGGTA AAGAAGAAGC TTGTCGCATC TTAGTGGACA ATCTGTGTGA CATGGAGATG 
GTCAACAAAG TGGGCCAAAC AGCCTTTGAC GTAGCAGATG AAGACATTTT AGGCTATTTA 
GAAGAGTTGC AAAAGAAGCA GAATCTGCTG CATAGTGAAA AACGGGAGAA GAAATCTCCT 
CTAATTGAGT CAACAGCCAA TATAGACAAT AATCAGTCAC AGAAGACCTT TAAAAACAAA 
GAGACACTGA TTATTGAGAC AGAGAAAAAT GCATCCCGTA TTGACTCTCT AGAACAAGAA 
AAGGTTGATG ATGAGGAAGA AGGAAAGAAA GATGAGTCTA GCTGCTCTAG CGAAGAAGAC 
GAAGAAGATG ACTCCGAGTC AGAAGCGGAA ACAGATAAGA CAAGACCCGT GGCTTCTGTA 
ACTAATGTCA GTACTGCAAG TACACCAGCA GCTCCTGTAG CTGTGACACC TACTGTCTCG 
TCTGGCCAGG CAACTCCCAC GTCACCTGTT AAAAAGTTTC CGACCTCCAC TACTAAAATT 
TCTCCCAAAG AAGAAGAGAG GAAAGATGAG TCTCCTGCAT CATGGAGGTT AGGACTCAGA 
AAGACTGGCA GTTACGGCGC ACTGGCCGAA ATCACAGCAT CTAAAGAGGC ACAGAAAGAA 
AAAGACATGA CAGGTGTCAT GCGTTCTGCA TCAAGCCCCA GGCTTTCCTC CTCTTTGGAT 
AACAAAGAAA AGGAAAAAGA TAGCAAAGGA ACTCGCCTCG CCTATGTCGC CCCTACAATA 
CCAAGACGGC TGGCCAGCAC GTCTGACATT GAAGAAAAAG AAAACAGAGA CTCTTTAAGT 
TTGCGAACAA GTAGTTCTTA CACAAGAAGG AAATGGGAAG ATGACCTCAA AAAGAATAGT 
TCAGTCAATG AAGGCTCAAC TTACCACAGA AGTACCTCAA ATCGTTTGTG GGCTGAGGAT 
AGTACTGAGA AGGAAAAGGA CAGTGTCCCC ACGGCAGTGA CCATTCCCGT TGCTCCAACT 
GTTGTAAACG CTGCGGCCTC TACCACAACC CTGACTACTA CTACTGCCGG CACTGTCTCC 
TCTACGTCAG AGGTCAGGGA GAGACGCAGA TCATACCTCA CTCCTGTTAG AGATGAAGAG 
TCTGAATCCC AAAGAAAAGC AAGATCCAGG CAAGCAAGAC AGTCGAGAAG ATCGACACAG 
GGAGTGACAT TAACTGATCT TCAGGAAGCG GAAAAAACAA TAGGACGAAG TCGTTCTATC 
AGAACCAGAG AACCAGAAAA TGAAGAAAAA GAAAAAGAGG AAAAAGAAAA GCAGGATAAA 
GAGAAGCAGG AAGAAAAGAA GGAGTCAGAA TTATCTAGAG AAGATGAATT AAAACAGAAA 
TACTCCAGAA CGTATGATGA GACCTGCCAG CGTTACAGGC CAGTGTCGAC TTCCAGTGTG 
ACCACTCCAT CCTCTTCGCT TTCCACCATG AGCAGCTCTC TCTACACATC GAGTCAGCTA 
AGCAGACCAA ACAGTCTCAT AGGGATAACT TCTGGCTACT CCAGAGGCTT AACAAAAGAA 
AATGAACGAG AGGGGGAGAA GAAAGAAGAG GAAAAAGAAG GAGAAGACAA ATCCCAGTCA 
AAATCAGTCA GAGAACGACG GAGGCCAAGA GAGAAAAGAA GATCTACAGG AGTTTCATTC 
TGGACACAAG ATAGTGATGA AAATGAACAA GAACAGCAGT CAGATACAGA AGAAGGATCC 
AATAAGAAAG AAACTCAGAC GGATTCCATT TCTAGATATG AAACCAGTTC CACTTCGACT 
GGTGAGCGGT CTGATTCCTC GCTGGGCCGC TCAGGATCAT ACAGTTACTT AGAGGAGAGA 
AAGCCTTACA GCAGCAGACT AGAGAAGGAC GTGTCGACTG ACTTTAAAAA GCTTTATGAA 
AAAATCCTAG CTGAAAATGA AAAGCTAAAA GTTCAGCTGC ATGACACAAA CATGGAACTG 
ACAGATCTTA AATTGCAGTT GGAAAAAGCC ACCCAGAGAC AAGAAAGGTT TGCTGATAGG 
TCACTATTGG AAATGGAAAA AAGGGAACGA AGAGCACTAG AAAGAAGAAT ATCTGAAATG 
GAAGAAGAGC TCAAAATGTT GCCAGACCTA AAAGCAGACA ACCAGAGGCT AAAGGATGAA 
AATGGGGCCT TGATCAGAGT TATAAGCAAA CTTTCCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R12A ( XM_007525650.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007525712.1 Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X1, mRNA.

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CloneID	OEr13673
Clone ID Related Accession (Same CDS sequence)	XM_007525650.2
Accession Version	XM_007525650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	protein phosphatase 1 regulatory subunit 12A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804197.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007525650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TCAAGCGCTG GATCGGCTCC 
GAGACGGACC TGGAGCCGCC GGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGACGAT 
GGCGCCGTCT TCCTGGCCGC CTGCTCCAGC GGCGACACGG ACGAGGTGCT CCGCCTGCTG 
CACCGCGGCG CCGACATCAA CTACGCCAAT GTGGACGGGC TCACCGCCCT GCACCAGGCT 
TGCATTGATG ACAATGTGGA AATGGTGAAG TTTCTGGTGG AAAATGGAGC AAATATTAAT 
CAACCTGATA ACGAAGGCTG GATACCACTG CATGCAGCTG CTTCTTGTGG ATATCTTGAT 
ATTGCAGAGT TTTTAATCGG CCAAGGAGCA CATGTAGGCG CTGTCAACAG TGAAGGGGAC 
ACTCCTTTAG ATATTGCTGA GGAGGAGGCG ATGGAAGAAC TACTGCAAAA TGAAGTGAAC 
CGACAAGGAG TTGACATAGA AGCAGCTCGA AAGGAAGAAG AGCGGATCAT GCTGAGAGAC 
GCCAGGCAGT GGCTGAACAG CGGTCACATC AGTGACGTCC GGCACGCCAA GTCCGGAGGC 
ACAGCGCTCC ACGTGGCTGC TGCTAAAGGC TACACAGAAG TTTTAAAACT TTTAATACAG 
GCAGGCTATG ATGTTAACGT CAAGGACTAT GATGGCTGGA CTCCTCTTCA CGCCGCGGCT 
CACTGGGGTA AAGAAGAAGC TTGTCGCATC TTAGTGGACA ATCTGTGTGA CATGGAGATG 
GTCAACAAAG TGGGCCAAAC AGCCTTTGAC GTAGCAGATG AAGACATTTT AGGCTATTTA 
GAAGAGTTGC AAAAGAAGCA GAATCTGCTG CATAGTGAAA AACGGGAGAA GAAATCTCCT 
CTAATTGAGT CAACAGCCAA TATAGACAAT AATCAGTCAC AGAAGACCTT TAAAAACAAA 
GAGACACTGA TTATTGAGAC AGAGAAAAAT GCATCCCGTA TTGACTCTCT AGAACAAGAA 
AAGGTTGATG ATGAGGAAGA AGGAAAGAAA GATGAGTCTA GCTGCTCTAG CGAAGAAGAC 
GAAGAAGATG ACTCCGAGTC AGAAGCGGAA ACAGATAAGA CAAGACCCGT GGCTTCTGTA 
ACTAATGTCA GTACTGCAAG TACACCAGCA GCTCCTGTAG CTGTGACACC TACTGTCTCG 
TCTGGCCAGG CAACTCCCAC GTCACCTGTT AAAAAGTTTC CGACCTCCAC TACTAAAATT 
TCTCCCAAAG AAGAAGAGAG GAAAGATGAG TCTCCTGCAT CATGGAGGTT AGGACTCAGA 
AAGACTGGCA GTTACGGCGC ACTGGCCGAA ATCACAGCAT CTAAAGAGGC ACAGAAAGAA 
AAAGACATGA CAGGTGTCAT GCGTTCTGCA TCAAGCCCCA GGCTTTCCTC CTCTTTGGAT 
AACAAAGAAA AGGAAAAAGA TAGCAAAGGA ACTCGCCTCG CCTATGTCGC CCCTACAATA 
CCAAGACGGC TGGCCAGCAC GTCTGACATT GAAGAAAAAG AAAACAGAGA CTCTTTAAGT 
TTGCGAACAA GTAGTTCTTA CACAAGAAGG AAATGGGAAG ATGACCTCAA AAAGAATAGT 
TCAGTCAATG AAGGCTCAAC TTACCACAGA AGTTACTCCT TTGGTAGAAG ACAAGATGAT 
TTGATTAGTT CTAGTGTTCC AAGCACCACA TCAACACCAA CAGTTACCTC TGCAGCTGGG 
CTTCAGAAAA GCCTGCTCCC CAGCTCAAGC ACTCCTACAA AGATTGCAGT GGGCTCTTCC 
TCAGCAGGCA CACAAAGCAG TACCTCAAAT CGTTTGTGGG CTGAGGATAG TACTGAGAAG 
GAAAAGGACA GTGTCCCCAC GGCAGTGACC ATTCCCGTTG CTCCAACTGT TGTAAACGCT 
GCGGCCTCTA CCACAACCCT GACTACTACT ACTGCCGGCA CTGTCTCCTC TACGTCAGAG 
GTCAGGGAGA GACGCAGATC ATACCTCACT CCTGTTAGAG ATGAAGAGTC TGAATCCCAA 
AGAAAAGCAA GATCCAGGCA AGCAAGACAG TCGAGAAGAT CGACACAGGG AGTGACATTA 
ACTGATCTTC AGGAAGCGGA AAAAACAATA GGACGAAGTC GTTCTATCAG AACCAGAGAA 
CCAGAAAATG AAGAAAAAGA AAAAGAGGAA AAAGAAAAGC AGGATAAAGA GAAGCAGGAA 
GAAAAGAAGG AGTCAGAATT ATCTAGAGAA GATGAATTAA AACAGAAATA CTCCAGAACG 
TATGATGAGA CCTGCCAGCG TTACAGGCCA GTGTCGACTT CCAGTGTGAC CACTCCATCC 
TCTTCGCTTT CCACCATGAG CAGCTCTCTC TACACATCGA GTCAGCTAAG CAGACCAAAC 
AGTCTCATAG GGATAACTTC TGGCTACTCC AGAGGCTTAA CAAAAGAAAA TGAACGAGAG 
GGGGAGAAGA AAGAAGAGGA AAAAGAAGGA GAAGACAAAT CCCAGTCAAA ATCAGTCAGA 
GAACGACGGA GGCCAAGAGA GAAAAGAAGA TCTACAGGAG TTTCATTCTG GACACAAGAT 
AGTGATGAAA ATGAACAAGA ACAGCAGTCA GATACAGAAG AAGGATCCAA TAAGAAAGAA 
ACTCAGACGG ATTCCATTTC TAGATATGAA ACCAGTTCCA CTTCGACTGG TGAGCGGTCT 
GATTCCTCGC TGGGCCGCTC AGGATCATAC AGTTACTTAG AGGAGAGAAA GCCTTACAGC 
AGCAGACTAG AGAAGGACGT GTCGACTGAC TTTAAAAAGC TTTATGAAAA AATCCTAGCT 
GAAAATGAAA AGCTAAAAGT TCAGCTGCAT GACACAAACA TGGAACTGAC AGATCTTAAA 
TTGCAGTTGG AAAAAGCCAC CCAGAGACAA GAAAGGTTTG CTGATAGGTC ACTATTGGAA 
ATGGAAAAAA GGGAACGAAG AGCACTAGAA AGAAGAATAT CTGAAATGGA AGAAGAGCTC 
AAAATGTTGC CAGACCTAAA AGCAGACAAC CAGAGGCTAA AGGATGAAAA TGGGGCCTTG 
ATCAGAGTTA TAAGCAAACT TTCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007525712.1
CDS	129..3218
Translation	MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLRLLHRGADINYANVDGLTALHQACIDDNVEMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHISDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNVKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKREKKSPLIESTANIDNNQSQKTFKNKETLIIETEKNASRIDSLEQEKVDDEEEGKKDESSCSSEEDEEDDSESEAETDKTRPVASVTNVSTASTPAAPVAVTPTVSSGQATPTSPVKKFPTSTTKISPKEEERKDESPASWRLGLRKTGSYGALAEITASKEAQKEKDMTGVMRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPTIPRRLASTSDIEEKENRDSLSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHRSYSFGRRQDDLISSSVPSTTSTPTVTSAAGLQKSLLPSSSTPTKIAVGSSSAGTQSSTSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTSEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSIRTREPENEEKEKEEKEKQDKEKQEEKKESELSREDELKQKYSRTYDETCQRYRPVSTSSVTTPSSSLSTMSSSLYTSSQLSRPNSLIGITSGYSRGLTKENEREGEKKEEEKEGEDKSQSKSVRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSTGERSDSSLGRSGSYSYLEERKPYSSRLEKDVSTDFKKLYEKILAENEKLKVQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK

Target ORF information:

RefSeq Version	XM_007525650.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007525650.2

ORF Insert Sequence:

ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TCAAGCGCTG GATCGGCTCC 
GAGACGGACC TGGAGCCGCC GGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGACGAT 
GGCGCCGTCT TCCTGGCCGC CTGCTCCAGC GGCGACACGG ACGAGGTGCT CCGCCTGCTG 
CACCGCGGCG CCGACATCAA CTACGCCAAT GTGGACGGGC TCACCGCCCT GCACCAGGCT 
TGCATTGATG ACAATGTGGA AATGGTGAAG TTTCTGGTGG AAAATGGAGC AAATATTAAT 
CAACCTGATA ACGAAGGCTG GATACCACTG CATGCAGCTG CTTCTTGTGG ATATCTTGAT 
ATTGCAGAGT TTTTAATCGG CCAAGGAGCA CATGTAGGCG CTGTCAACAG TGAAGGGGAC 
ACTCCTTTAG ATATTGCTGA GGAGGAGGCG ATGGAAGAAC TACTGCAAAA TGAAGTGAAC 
CGACAAGGAG TTGACATAGA AGCAGCTCGA AAGGAAGAAG AGCGGATCAT GCTGAGAGAC 
GCCAGGCAGT GGCTGAACAG CGGTCACATC AGTGACGTCC GGCACGCCAA GTCCGGAGGC 
ACAGCGCTCC ACGTGGCTGC TGCTAAAGGC TACACAGAAG TTTTAAAACT TTTAATACAG 
GCAGGCTATG ATGTTAACGT CAAGGACTAT GATGGCTGGA CTCCTCTTCA CGCCGCGGCT 
CACTGGGGTA AAGAAGAAGC TTGTCGCATC TTAGTGGACA ATCTGTGTGA CATGGAGATG 
GTCAACAAAG TGGGCCAAAC AGCCTTTGAC GTAGCAGATG AAGACATTTT AGGCTATTTA 
GAAGAGTTGC AAAAGAAGCA GAATCTGCTG CATAGTGAAA AACGGGAGAA GAAATCTCCT 
CTAATTGAGT CAACAGCCAA TATAGACAAT AATCAGTCAC AGAAGACCTT TAAAAACAAA 
GAGACACTGA TTATTGAGAC AGAGAAAAAT GCATCCCGTA TTGACTCTCT AGAACAAGAA 
AAGGTTGATG ATGAGGAAGA AGGAAAGAAA GATGAGTCTA GCTGCTCTAG CGAAGAAGAC 
GAAGAAGATG ACTCCGAGTC AGAAGCGGAA ACAGATAAGA CAAGACCCGT GGCTTCTGTA 
ACTAATGTCA GTACTGCAAG TACACCAGCA GCTCCTGTAG CTGTGACACC TACTGTCTCG 
TCTGGCCAGG CAACTCCCAC GTCACCTGTT AAAAAGTTTC CGACCTCCAC TACTAAAATT 
TCTCCCAAAG AAGAAGAGAG GAAAGATGAG TCTCCTGCAT CATGGAGGTT AGGACTCAGA 
AAGACTGGCA GTTACGGCGC ACTGGCCGAA ATCACAGCAT CTAAAGAGGC ACAGAAAGAA 
AAAGACATGA CAGGTGTCAT GCGTTCTGCA TCAAGCCCCA GGCTTTCCTC CTCTTTGGAT 
AACAAAGAAA AGGAAAAAGA TAGCAAAGGA ACTCGCCTCG CCTATGTCGC CCCTACAATA 
CCAAGACGGC TGGCCAGCAC GTCTGACATT GAAGAAAAAG AAAACAGAGA CTCTTTAAGT 
TTGCGAACAA GTAGTTCTTA CACAAGAAGG AAATGGGAAG ATGACCTCAA AAAGAATAGT 
TCAGTCAATG AAGGCTCAAC TTACCACAGA AGTTACTCCT TTGGTAGAAG ACAAGATGAT 
TTGATTAGTT CTAGTGTTCC AAGCACCACA TCAACACCAA CAGTTACCTC TGCAGCTGGG 
CTTCAGAAAA GCCTGCTCCC CAGCTCAAGC ACTCCTACAA AGATTGCAGT GGGCTCTTCC 
TCAGCAGGCA CACAAAGCAG TACCTCAAAT CGTTTGTGGG CTGAGGATAG TACTGAGAAG 
GAAAAGGACA GTGTCCCCAC GGCAGTGACC ATTCCCGTTG CTCCAACTGT TGTAAACGCT 
GCGGCCTCTA CCACAACCCT GACTACTACT ACTGCCGGCA CTGTCTCCTC TACGTCAGAG 
GTCAGGGAGA GACGCAGATC ATACCTCACT CCTGTTAGAG ATGAAGAGTC TGAATCCCAA 
AGAAAAGCAA GATCCAGGCA AGCAAGACAG TCGAGAAGAT CGACACAGGG AGTGACATTA 
ACTGATCTTC AGGAAGCGGA AAAAACAATA GGACGAAGTC GTTCTATCAG AACCAGAGAA 
CCAGAAAATG AAGAAAAAGA AAAAGAGGAA AAAGAAAAGC AGGATAAAGA GAAGCAGGAA 
GAAAAGAAGG AGTCAGAATT ATCTAGAGAA GATGAATTAA AACAGAAATA CTCCAGAACG 
TATGATGAGA CCTGCCAGCG TTACAGGCCA GTGTCGACTT CCAGTGTGAC CACTCCATCC 
TCTTCGCTTT CCACCATGAG CAGCTCTCTC TACACATCGA GTCAGCTAAG CAGACCAAAC 
AGTCTCATAG GGATAACTTC TGGCTACTCC AGAGGCTTAA CAAAAGAAAA TGAACGAGAG 
GGGGAGAAGA AAGAAGAGGA AAAAGAAGGA GAAGACAAAT CCCAGTCAAA ATCAGTCAGA 
GAACGACGGA GGCCAAGAGA GAAAAGAAGA TCTACAGGAG TTTCATTCTG GACACAAGAT 
AGTGATGAAA ATGAACAAGA ACAGCAGTCA GATACAGAAG AAGGATCCAA TAAGAAAGAA 
ACTCAGACGG ATTCCATTTC TAGATATGAA ACCAGTTCCA CTTCGACTGG TGAGCGGTCT 
GATTCCTCGC TGGGCCGCTC AGGATCATAC AGTTACTTAG AGGAGAGAAA GCCTTACAGC 
AGCAGACTAG AGAAGGACGT GTCGACTGAC TTTAAAAAGC TTTATGAAAA AATCCTAGCT 
GAAAATGAAA AGCTAAAAGT TCAGCTGCAT GACACAAACA TGGAACTGAC AGATCTTAAA 
TTGCAGTTGG AAAAAGCCAC CCAGAGACAA GAAAGGTTTG CTGATAGGTC ACTATTGGAA 
ATGGAAAAAA GGGAACGAAG AGCACTAGAA AGAAGAATAT CTGAAATGGA AGAAGAGCTC 
AAAATGTTGC CAGACCTAAA AGCAGACAAC CAGAGGCTAA AGGATGAAAA TGGGGCCTTG 
ATCAGAGTTA TAAGCAAACT TTCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PPP1R12A cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム