BRF2 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRF2
Entrez Gene ID	103107872
Full Name	BRF2, RNA polymerase III transcription initiation factor subunit
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007516694.2	XP_007516756.1	transcription factor IIIB 50 kDa subunit

Pathways from BioSystems
Gene Ontology

The following BRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr04431	XM_007516694.2 Latest version!	Erinaceus europaeus BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit (BRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRF2 ( XM_007516694.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007516756.1 Erinaceus europaeus BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit (BRF2), mRNA.

Price & Availability↑

CloneID	OEr04431
Clone ID Related Accession (Same CDS sequence)	XM_007516694.2
Accession Version	XM_007516694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	transcription factor IIIB 50 kDa subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006803981.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007516694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCC GAGGCCGCTG CCCAGACTGT GGCTCCAGCG AGCTCGTGGA GGACTCGCAC 
TACGCGCAGA GTCAGCTGGT CTGCGCCGAC TGCGGCTGCG TGCTCACCGA GGGCGTCCTC 
ACCACCACCT TCAGCGACGA GGGCAGCCTC CGCGAAGTAA CATATTCCCG AAGCACAGGG 
GAAAACGAAC AAGTTAGTCG AAGCCAACAA CGAGATCTCC GCCGAGTAAG AGACCTCTGC 
CGAGTCTTGA AGTTGCCATC AACGTTTGAG GACACAGCAG TTGCCTACTA TCAGCAGGCA 
TACCGGCACT CAGGCATCCG TACTGCCAGG CTGCAAAAGA AGGAAGTGTT GGTTGGCTGC 
TGTGTCTTAA TTACCTGCCG GCAGCACAAC TGGCCTCTTA CCATGGGAAC CATCTGCACC 
CTGCTGTATG CAGATTTGGA TGTGTTTTCT GGCACCTACA TGCAGATAGT GAAGCTCCTG 
GGATTGGATG TGCCATCTCT CTGCTTAGCA GATTTGGTGA AGACATACTG TAGCAGCTTC 
AAACTGTTCC AGGCCTCTGC CTCTGTGCCC ACCAAATACG TGGAAGATAA AGAGAAGATG 
CTGTCACGGA CATTGCAGTT GGTGGAACTG GCAGATGAGA CATGGCTGGT GACTGGGCGA 
CACCCTTTGC CTGTCATCAC TGCAGCTGCC TTCCTGGCTT GGCAGTCCTT GCAACCTTCA 
AATCGATTGT CGTGCTCCCT CACCCGATTC TGTAAATTGG CCAACGTGGA GCTGCCCTAT 
CCTGCTTCTG CCCGCCTGCA GGAGCTCCTG ACTGTGCTGC TGCAGATGTC AGAGCAGCTG 
GTCTGGTTGC AGTTTCTGAA ACTTGACAAA CGCGCTGTGG TAAAGCACAT TGGTGACCTT 
CTTCAGCACC GCCATATGCT GATCCGCAAG GCCTTTCGGG ATGGAACAGA GCTGGAGGCT 
CAAGAGAAGA AACGGCAGCA GCAGGGAGAG GGAGAGGGAC AAGAGGAGGT GGGGAGTAGT 
TTCTTAGGTT TGCCAGAGGG AAAGCGACCA CCTAGTCCTA CTCCTCTTCT CCCTCCCTGC 
ATGTTGAAGC CCCCAAAGCG GATCTGTCCC CCACCTCCCA TTTCCACGGT TACAGGAGAT 
GAGGACATTT CTGACAGTGA AATAGAGCAG TATTTGCGTA CCCCAGAGGA AGTGAGGGAC 
TTTCAGAAAG CCCAGGCTGC TAGGCAGGAT GCCATGAATG TTCCTAATCC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007516756.1
CDS	62..1318
Translation	MPGRGRCPDCGSSELVEDSHYAQSQLVCADCGCVLTEGVLTTTFSDEGSLREVTYSRSTGENEQVSRSQQRDLRRVRDLCRVLKLPSTFEDTAVAYYQQAYRHSGIRTARLQKKEVLVGCCVLITCRQHNWPLTMGTICTLLYADLDVFSGTYMQIVKLLGLDVPSLCLADLVKTYCSSFKLFQASASVPTKYVEDKEKMLSRTLQLVELADETWLVTGRHPLPVITAAAFLAWQSLQPSNRLSCSLTRFCKLANVELPYPASARLQELLTVLLQMSEQLVWLQFLKLDKRAVVKHIGDLLQHRHMLIRKAFRDGTELEAQEKKRQQQGEGEGQEEVGSSFLGLPEGKRPPSPTPLLPPCMLKPPKRICPPPPISTVTGDEDISDSEIEQYLRTPEEVRDFQKAQAARQDAMNVPNPP

Target ORF information:

RefSeq Version	XM_007516694.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit (BRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007516694.2

ORF Insert Sequence:

ATGCCGGGCC GAGGCCGCTG CCCAGACTGT GGCTCCAGCG AGCTCGTGGA GGACTCGCAC 
TACGCGCAGA GTCAGCTGGT CTGCGCCGAC TGCGGCTGCG TGCTCACCGA GGGCGTCCTC 
ACCACCACCT TCAGCGACGA GGGCAGCCTC CGCGAAGTAA CATATTCCCG AAGCACAGGG 
GAAAACGAAC AAGTTAGTCG AAGCCAACAA CGAGATCTCC GCCGAGTAAG AGACCTCTGC 
CGAGTCTTGA AGTTGCCATC AACGTTTGAG GACACAGCAG TTGCCTACTA TCAGCAGGCA 
TACCGGCACT CAGGCATCCG TACTGCCAGG CTGCAAAAGA AGGAAGTGTT GGTTGGCTGC 
TGTGTCTTAA TTACCTGCCG GCAGCACAAC TGGCCTCTTA CCATGGGAAC CATCTGCACC 
CTGCTGTATG CAGATTTGGA TGTGTTTTCT GGCACCTACA TGCAGATAGT GAAGCTCCTG 
GGATTGGATG TGCCATCTCT CTGCTTAGCA GATTTGGTGA AGACATACTG TAGCAGCTTC 
AAACTGTTCC AGGCCTCTGC CTCTGTGCCC ACCAAATACG TGGAAGATAA AGAGAAGATG 
CTGTCACGGA CATTGCAGTT GGTGGAACTG GCAGATGAGA CATGGCTGGT GACTGGGCGA 
CACCCTTTGC CTGTCATCAC TGCAGCTGCC TTCCTGGCTT GGCAGTCCTT GCAACCTTCA 
AATCGATTGT CGTGCTCCCT CACCCGATTC TGTAAATTGG CCAACGTGGA GCTGCCCTAT 
CCTGCTTCTG CCCGCCTGCA GGAGCTCCTG ACTGTGCTGC TGCAGATGTC AGAGCAGCTG 
GTCTGGTTGC AGTTTCTGAA ACTTGACAAA CGCGCTGTGG TAAAGCACAT TGGTGACCTT 
CTTCAGCACC GCCATATGCT GATCCGCAAG GCCTTTCGGG ATGGAACAGA GCTGGAGGCT 
CAAGAGAAGA AACGGCAGCA GCAGGGAGAG GGAGAGGGAC AAGAGGAGGT GGGGAGTAGT 
TTCTTAGGTT TGCCAGAGGG AAAGCGACCA CCTAGTCCTA CTCCTCTTCT CCCTCCCTGC 
ATGTTGAAGC CCCCAAAGCG GATCTGTCCC CCACCTCCCA TTTCCACGGT TACAGGAGAT 
GAGGACATTT CTGACAGTGA AATAGAGCAG TATTTGCGTA CCCCAGAGGA AGTGAGGGAC 
TTTCAGAAAG CCCAGGCTGC TAGGCAGGAT GCCATGAATG TTCCTAATCC TCCCTAG