NF1 cDNA ORF clone, Equus caballus(horse)

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb45493 XM_023653065.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHb33975 XM_014741967.1
Latest version!
Equus caballus neurofibromin 1 (NF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45484 XM_023653055.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45485 XM_023653057.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45486 XM_023653058.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45487 XM_023653059.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45488 XM_023653060.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45489 XM_023653061.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45490 XM_023653062.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45491 XM_023653063.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHb45492 XM_023653064.1
Latest version!
Equus caballus neurofibromin 1 (NF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb45493
    Clone ID Related Accession (Same CDS sequence) XM_023653065.1
    Accession Version XM_023653065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTTGGTG AAACACTTCA TAAAGCGGTG CAGGGTTGTG GAGCACACCC GGCGATACGC 
    ATGGCACCGA GTCTTACATT TAAAGAAAAA GTAACAAGCC TTAAATTTAA AGAAAAACCT
    ACAGACCTGG AGACAAGAAG CTATAAGTAT CTTCTTTTGT CTATGGTGAA ACTAATTCAT
    GCAGATCCAA AGCTCTTGCT TTGTAATCCC AGAAAACAGG GGCCTGAAAC CCAAGGCAGT
    ACAGCAGAAT TAATTACAGG GCTCGTCCAG CTGGTCCCTC AGTCACACAT GCCAGAGATT
    GCTCAGGAAG CAATGGAGGC TCTGCTGGTG CTTCATCAGT TAGACAGCAT CGATTTGTGG
    AATCCTGATG CTCCTGTGGA AACATTTTGG GAAATTAGCT CACAAATGCT TTTTTACATC
    TGCAAGAAGT TAACTAGCCA GCAAATGCTT AGTAGCACAG AAATTCTCAA ATGGTTGCGG
    GAAATTTTGA TCTGCAGGAA TAAATTTCTT CTTAAAAATA AGCAGGCAGA TAGGAGTTCC
    TGTCACTTTC TCTTCCTTTA TGGGGTAGGA TGTGATGTTC CTTCCAGTGG AAATACCAGT
    CAAATGTCCA TGGATCATGA AGAGTTATTA CGTACTTATA CTCCTGGAGC CTCTCTCCGG
    AAGGGAAAAG GAAACTCATC CATGGATAGT GCGGCAGGGT GCAGTGGAAC GCCGCCGATA
    TGCCGGCAAG CCCAGACCAA ACTGGAAGTG GCCCTGTACA TGTTTCTGTG GAGCCCTGAC
    ACCGAAGCTG TTCTGGTTGC CATGTCCTGT TTCCGCCACC TCTGTGAGGA AGCGGATATC
    CGGTGTGGGG TGGACGAAGT ATCGGTGCAC AACTTCTTGC CGAACTATAA CACATTCATG
    GAATTTGCGT CTGTCAGCAA TATGATGTCA ACAGGAAGAG CAGCACTTCA GAAAAGAGTG
    ATGGCACTAC TGAGGCGCAT TGAACATCCC ACTGCAGGAA ACACTGAGGC TTGGGAAGAT
    ACACATGCAA AGTGGGAACA AGCTACAAAA CTAATCCTCA ACTATCCAAA AGCCAAAATG
    GAAGATGGCC AGGCTGCTGA AAGCCTTCAT AAGACCATTG TTAAGAGGCG AATGTCTCAT
    GTGAGTGGAG GAGGATCCAT AGATTTGTCT GACACGGACT CCCTTCAGGA ATGGATCAAC
    ATGACCGGCT TCCTCTGTGC CCTTGGGGGA GTGTGCCTGC AGCAGAGAAG CAATTCTGGC
    CTGGCAACCT ATAGCCCACC CATGGGCCCA GTCAGTGAAC GCAAGGGTTC CATGATTTCA
    GTGATGTCCT CAGAGGGAAA TGCAGACACA CCTGTCAGCA AATTTATGGA TCGGCTGCTA
    TCCTTAATGG TGTGTAACCA TGAGAAAGTG GGACTTCAAA TACGCACCAA CGTTAAGGAT
    CTGGTGGGTT TAGAATTGAG TCCTGCTCTT TATCCAATGC TGTTTAACAA ATTGAAGAAC
    ACCATCAGCA AGTTTTTTGA CTCCCAAGGA CAGGTTTTAT TGACTGACAC CAATACTCAA
    TTTGTAGAGC AAACCATAGC TATAATGAAG AACTTACTAG ATAATCATAC TGAAGGCAGC
    TCCGAACATC TGGGGCAAGC TAGCATTGAA ACGATGATGT TAAATCTGGT CAGATATGTT
    CGTGTACTTG GGAACATGGT CCATGCAATT CAAATAAAAA CGAAACTGTG TCAGCTAGTC
    GAGGTAATGA TGGCAAGAAG AGATGACCTC TCATTTTGTC AAGAGATGAA ATTTAGGAAT
    AAGATGGTAG AATATCTGAC AGATTGGGTT ATGGGAACAT CAAACCAAGC AGCAGATGAT
    GATGTAAAAT GTCTTACAAG AGATCTGGAC CAGGCAAGCA TGGAAGCAGT AGTTTCACTC
    CTGGCTGGTC TCCCGCTGCA GCCTGAGGAA GGAGATGGTG TGGAACTCAT GGAAGCCAAG
    TCACAGCTAT TTCTTAAGTA TTTCACATTG TTTATGAACC TTTTGAATGA CTGTAGTGAA
    GTTGAAGATG AAAATGCACA AACAGGTGGC AGGAAGCGTG GCATGTCTCG GAGGCTGGCC
    TCACTGAGGC ACTGCACAGT CCTTGCAATG TCAAACTTAC TTAACGCTAA CGTGGACAGT
    GGCCTCATGC ACTCCATAGG TTTAGGTTAT CACAAAGATC TCCAGACAAG AGCTACATTT
    ATGGAAGTTC TGACAAAAAT CCTCCAACAA GGCACAGAAT TTGACACACT TGCAGAAACG
    GTGTTGGCAG ATCGGTTTGA GAGGCTGGTG GAGTTGGTCA CAATGATGGG TGATCAGGGA
    GAGCTCCCTA TAGCCATGGC TCTGGCCAAT GTGGTCCCTT GTTCTCAGTG GGATGAACTA
    GCTCGAGTTC TAGTGACTCT CTTTGATTCT CGGCATTTAC TCTACCAACT GCTCTGGAAC
    ATGTTTTCTA AGGAAGTAGA ATTGGCAGAC TCCATGCAGA CTCTTTTCCG AGGCAACAGC
    TTAGCCAGTA AAATAATGAC CTTCTGTTTC AAGGTATATG GTGCTACCTA CCTACAAAAA
    CTCCTGGATC CTTTGTTACG AATTGTGATC ACATCCTCTG ATTGGCAACA TGTTAGCTTT
    GAAGTGGATC CTACCAGGTT AGAACAGTCA GAGAGCCTTG AGGAGAACCA GCGGAGCCTC
    CTTCAGATGA CTGAAAAGTT CTTCCACGCC ATCATCAGTT CCTCCTCGGA ATTCCCCCCT
    CAGCTTCGAA GTGTGTGCCA CTGTCTATAC CAGGCAACTT GCCACTCCCT ACTGAATACA
    GCTACAGTAA AAGAAAAAAA GGAAAACAAA AAATCAGTGG TTAGCCAGCG TTTCCCTCAG
    AACAGCATCG GTGCAGTGGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATCGTC
    TCACCGTATG AAGCAGGCAT TTTAGATAAA AAGCCACCAC CTAGAATCGA AAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TTCTCTTCAC AAAAGAAGAG
    CATATGCGGC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ATGCAGCACG AAGGTTTTTC
    CTTGATATAG CATCCGATTG TCCTACAAGT GATGCAGTGA ATCACAGTCT TTCCTTCATC
    AGCGATGGCA ACGTGCTGGC TCTACATCGT CTCCTCTGGA ACAATCAAGA GAAGATTGGC
    CAGTATCTTT CCAGCAATAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCAACACTTC TTGCATACCT GGGTCCTCCA GAGCACAAAC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTC GAGGAATTTA TGACTAGGCA TCAAGTACAT
    GAAAAAGAAG AGTTCAAGGC CTTGAAAACA TTAAGTATTT TCTACCAAGC TGGGACTTCC
    AGAGCTGGGA ATCCTATATT TTATTATGTT GCACGGAGGT TCAAAACTGG TCAAATCAAT
    GGCGATTTGC TGATCTACCA TGTCTTGCTG ACTCTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACCGGG CCTAGCAATC GCTTTAAAAC GGACTTTCTC
    TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT GCTTATGACA ATGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAC ACCAAGTATC ACGAGCGGCT GCTCACTGGC
    CTCAAAGGCA GCAAAAGGCT CATTTTCATA GACTGTCCTG GGAAGCTGGC TGAGCACGTA
    GAGCACGAAC AACAGAAACT ACCTGCTGCC ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG
    TTCCACAATG CTCTCAAGCT GGCTCACAAA GACACCAAGG TTTCCATTAA AGTTGGTTCT
    ACTGCTGTTC AAGTGACCTC AGCAGAGCGA ACGAAAGTCC TAGGGCAATC AGTCTTTCTA
    AACGATATCT ACTATGCTTC GGAAATTGAA GAGATCTGCC TGGTGGATGA GAACCAGTTC
    ACCTTAACCA TTGCAAACCA GGGCACACCG CTCACCTTCA TGCACCAGGA GTGTGAAGCC
    ATCGTCCAGT CCATCATTCA CATCCGGACA CGCTGGGAGC TGTCACAGCC CGACTCCATC
    CCCCAGCACA CCAAGATCCG GCCAAAAGAT GTCCCTGGGA CACTGCTCAA TATCGCGTTA
    CTTAATTTAG GCAGTTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACTTGTA CCTTTAATTT AAAAATCGAG GGCCAGTTGC TAGAGACGTC AGGTTTATGT
    ATCCCTGCCA ACAACACCCT CTTTATTGTC TCTATTAGTA AGACACTGGC AGCCAATGAG
    CCACACCTCA CCTTAGAATT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAACTGAAAC ACCTTTGTTT GGAATACATG ACTCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCACA ATGATGATGC CAAAAGACAA AGAGTTACTG CTATTCTTGA TAAGCTGATA
    ACAATGACCA TAAATGAGAA ACAGATGTAC CCATCTATTC AAGCAAAAAT ATGGGGGAGC
    CTTGGGCAGA TTACAGATCT CCTTGATGTT GTGCTAGACA GTTTCATCAA AACCAGTGCA
    ACAGGTGGCT TGGGATCAAT AAAAGCCGAG GTGATGGCAG ATACTGCTGT AGCTCTGGCG
    TCTGGAAATG TGAAATTGGT TTCTAGCAAG GTTATTGGAA GGATGTGCAA AATAATTGAC
    AAGACATGCT TATCTCCAAC TCCTACTTTA GAACAGCATC TTATGTGGGA TGACATTGCG
    ATTCTAGCGC GCTACATGCT GATGTTGTCC TTCAACAACT CCCTCGACGT GGCGGCTCAT
    CTCCCCTACC TCTTCCACGT TGTGACTTTC TTAGTAGCCA CAGGGCCACT CTCCCTTAGA
    GCTTCCACAC ATGGATTGGT CATTAATATC ATTCACTCTC TGTGCACTTG TTCACAGCTT
    CATTTTAGTG AGGAGACCAA GCAAGTTTTG AGACTCAGTT TGACAGAGTT CTCATTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC
    TCCAGTTACC GGGACAGGTC GTTCTCTCCT GGCTCCTACG AGAGGGAGAC TTTTGCTTTG
    ACATCCTTGG AAACAGTCAC GGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGGTT TGCATTCCAG
    TACAACCCGT CCCTGCAACC GAGAGCTCTG GTCGTCTTTG GCTGCATTAG TAAGCGAGTG
    TCTCACGGGC AGATAAAGCA GATTATCCGC ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA
    AAAGGACCTG ATACTTACAA CAGTCAAGTT CTGATAGAAG CTACAGTAAT AGCACTAACC
    AAATTACAAC CACTTCTTAA TAAGGACTCC CCTCTGCACA AAGCCCTCTT TTGGGTAGCT
    GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC TTGTATTCAG CAGGCACCGC ACTTCTTGAG
    CAAAACCTGC ACACCTTAGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC AGAGGAAGTA
    TTTATGGCAA TTCGGAATCC TCTGGAATGG CACTGCAAGC AAATGGATCA TTTTGTTGGA
    CTCAATTTCA ACTCTAACTT TAACTTTGCA CTGGTTGGAC ACCTCTTAAA AGGGTACAGA
    CATCCTTCAC CTGCTATTGT TGCACGAACA GTCAGAATTT TGCATACACT ACTAACTCTG
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CGCAGAGCGT GGCTTACTTA
    GCAGCTTTAC TCACAGTATC TGAGGAGGTT CGAAGTCGCT GTAGCCTAAA ACATAGAAAG
    TCTCTTCTTC TTACTGATAT TTCAATGGAA AATATTCCTA TGGATACATA TCCCATTCAT
    CATGGCGACC CCAGCTATAG GACACTAAAG GAGAGTCAGC CATGGTCCTC TCCCAAAGGC
    TCGGAAGGGT ACCTTGCAGC CACCTATCCA ACCGTGGGCC AGACCAGCCC CCGAGCCCGG
    AAATCCATGA GCTTGGACAT GGGGCAGCCT TCTCAGGCCA ACACTAAGAA GTTGCTGGGA
    ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACGAAGG CTCCTAAAAG GCAAGAAGTG
    GAATCAGGGA TCACAACACC CCCCAAAATG AGGAGAGTAG CAGAAACTGA TTATGAAATG
    GAAACTCAAA GGATTTCCTC ATCACAACAG CACCCACATT TACGTAAAGT TTCAGTGTCT
    GAATCAAATG TTCTCTTGGA TGAAGAAGTA CTTACTGACC CGAAGATCCA GGCATTGCTG
    CTTACTGTTC TGGCTACACT GGTGAAATAC ACCACGGATG AGTTTGATCA ACGAATTCTT
    TATGAATACT TAGCAGAAGC CAGTGTTGTT TTCCCCAAAG TTTTTCCTGT TGTGCACAAT
    TTGTTGGACT CCAAGATCAA CACCCTGTTG TCATTGTGCC AAGATCCAAA TTTGTTAAAT
    CCGATTCATG GGATTGTGCA GAGTGTGGTA TACTACGAGG AGTCCCCACC ACAGTACCAG
    ACGTCTTACC TGCAGAGTTT TGGTTTTAAT GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA
    AAGCAAACAC AAATCCCAGA CTATGCTGAG CTTATTGTTA AGTTTCTTGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTATC CTCCTGCAGT GCAGAGCCAG CTTAGTATTA CTGCTAACCT TAACCTTTCC
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAACGTT
    GAACTCTCCC CAACCACTGG GCACTGTAAC AGTGGACGAA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508833.1
    CDS83..7285
    Translation

    Target ORF information:

    RefSeq Version XM_023653065.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTTGGTG AAACACTTCA TAAAGCGGTG CAGGGTTGTG GAGCACACCC GGCGATACGC 
    ATGGCACCGA GTCTTACATT TAAAGAAAAA GTAACAAGCC TTAAATTTAA AGAAAAACCT
    ACAGACCTGG AGACAAGAAG CTATAAGTAT CTTCTTTTGT CTATGGTGAA ACTAATTCAT
    GCAGATCCAA AGCTCTTGCT TTGTAATCCC AGAAAACAGG GGCCTGAAAC CCAAGGCAGT
    ACAGCAGAAT TAATTACAGG GCTCGTCCAG CTGGTCCCTC AGTCACACAT GCCAGAGATT
    GCTCAGGAAG CAATGGAGGC TCTGCTGGTG CTTCATCAGT TAGACAGCAT CGATTTGTGG
    AATCCTGATG CTCCTGTGGA AACATTTTGG GAAATTAGCT CACAAATGCT TTTTTACATC
    TGCAAGAAGT TAACTAGCCA GCAAATGCTT AGTAGCACAG AAATTCTCAA ATGGTTGCGG
    GAAATTTTGA TCTGCAGGAA TAAATTTCTT CTTAAAAATA AGCAGGCAGA TAGGAGTTCC
    TGTCACTTTC TCTTCCTTTA TGGGGTAGGA TGTGATGTTC CTTCCAGTGG AAATACCAGT
    CAAATGTCCA TGGATCATGA AGAGTTATTA CGTACTTATA CTCCTGGAGC CTCTCTCCGG
    AAGGGAAAAG GAAACTCATC CATGGATAGT GCGGCAGGGT GCAGTGGAAC GCCGCCGATA
    TGCCGGCAAG CCCAGACCAA ACTGGAAGTG GCCCTGTACA TGTTTCTGTG GAGCCCTGAC
    ACCGAAGCTG TTCTGGTTGC CATGTCCTGT TTCCGCCACC TCTGTGAGGA AGCGGATATC
    CGGTGTGGGG TGGACGAAGT ATCGGTGCAC AACTTCTTGC CGAACTATAA CACATTCATG
    GAATTTGCGT CTGTCAGCAA TATGATGTCA ACAGGAAGAG CAGCACTTCA GAAAAGAGTG
    ATGGCACTAC TGAGGCGCAT TGAACATCCC ACTGCAGGAA ACACTGAGGC TTGGGAAGAT
    ACACATGCAA AGTGGGAACA AGCTACAAAA CTAATCCTCA ACTATCCAAA AGCCAAAATG
    GAAGATGGCC AGGCTGCTGA AAGCCTTCAT AAGACCATTG TTAAGAGGCG AATGTCTCAT
    GTGAGTGGAG GAGGATCCAT AGATTTGTCT GACACGGACT CCCTTCAGGA ATGGATCAAC
    ATGACCGGCT TCCTCTGTGC CCTTGGGGGA GTGTGCCTGC AGCAGAGAAG CAATTCTGGC
    CTGGCAACCT ATAGCCCACC CATGGGCCCA GTCAGTGAAC GCAAGGGTTC CATGATTTCA
    GTGATGTCCT CAGAGGGAAA TGCAGACACA CCTGTCAGCA AATTTATGGA TCGGCTGCTA
    TCCTTAATGG TGTGTAACCA TGAGAAAGTG GGACTTCAAA TACGCACCAA CGTTAAGGAT
    CTGGTGGGTT TAGAATTGAG TCCTGCTCTT TATCCAATGC TGTTTAACAA ATTGAAGAAC
    ACCATCAGCA AGTTTTTTGA CTCCCAAGGA CAGGTTTTAT TGACTGACAC CAATACTCAA
    TTTGTAGAGC AAACCATAGC TATAATGAAG AACTTACTAG ATAATCATAC TGAAGGCAGC
    TCCGAACATC TGGGGCAAGC TAGCATTGAA ACGATGATGT TAAATCTGGT CAGATATGTT
    CGTGTACTTG GGAACATGGT CCATGCAATT CAAATAAAAA CGAAACTGTG TCAGCTAGTC
    GAGGTAATGA TGGCAAGAAG AGATGACCTC TCATTTTGTC AAGAGATGAA ATTTAGGAAT
    AAGATGGTAG AATATCTGAC AGATTGGGTT ATGGGAACAT CAAACCAAGC AGCAGATGAT
    GATGTAAAAT GTCTTACAAG AGATCTGGAC CAGGCAAGCA TGGAAGCAGT AGTTTCACTC
    CTGGCTGGTC TCCCGCTGCA GCCTGAGGAA GGAGATGGTG TGGAACTCAT GGAAGCCAAG
    TCACAGCTAT TTCTTAAGTA TTTCACATTG TTTATGAACC TTTTGAATGA CTGTAGTGAA
    GTTGAAGATG AAAATGCACA AACAGGTGGC AGGAAGCGTG GCATGTCTCG GAGGCTGGCC
    TCACTGAGGC ACTGCACAGT CCTTGCAATG TCAAACTTAC TTAACGCTAA CGTGGACAGT
    GGCCTCATGC ACTCCATAGG TTTAGGTTAT CACAAAGATC TCCAGACAAG AGCTACATTT
    ATGGAAGTTC TGACAAAAAT CCTCCAACAA GGCACAGAAT TTGACACACT TGCAGAAACG
    GTGTTGGCAG ATCGGTTTGA GAGGCTGGTG GAGTTGGTCA CAATGATGGG TGATCAGGGA
    GAGCTCCCTA TAGCCATGGC TCTGGCCAAT GTGGTCCCTT GTTCTCAGTG GGATGAACTA
    GCTCGAGTTC TAGTGACTCT CTTTGATTCT CGGCATTTAC TCTACCAACT GCTCTGGAAC
    ATGTTTTCTA AGGAAGTAGA ATTGGCAGAC TCCATGCAGA CTCTTTTCCG AGGCAACAGC
    TTAGCCAGTA AAATAATGAC CTTCTGTTTC AAGGTATATG GTGCTACCTA CCTACAAAAA
    CTCCTGGATC CTTTGTTACG AATTGTGATC ACATCCTCTG ATTGGCAACA TGTTAGCTTT
    GAAGTGGATC CTACCAGGTT AGAACAGTCA GAGAGCCTTG AGGAGAACCA GCGGAGCCTC
    CTTCAGATGA CTGAAAAGTT CTTCCACGCC ATCATCAGTT CCTCCTCGGA ATTCCCCCCT
    CAGCTTCGAA GTGTGTGCCA CTGTCTATAC CAGGCAACTT GCCACTCCCT ACTGAATACA
    GCTACAGTAA AAGAAAAAAA GGAAAACAAA AAATCAGTGG TTAGCCAGCG TTTCCCTCAG
    AACAGCATCG GTGCAGTGGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATCGTC
    TCACCGTATG AAGCAGGCAT TTTAGATAAA AAGCCACCAC CTAGAATCGA AAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TTCTCTTCAC AAAAGAAGAG
    CATATGCGGC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ATGCAGCACG AAGGTTTTTC
    CTTGATATAG CATCCGATTG TCCTACAAGT GATGCAGTGA ATCACAGTCT TTCCTTCATC
    AGCGATGGCA ACGTGCTGGC TCTACATCGT CTCCTCTGGA ACAATCAAGA GAAGATTGGC
    CAGTATCTTT CCAGCAATAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCAACACTTC TTGCATACCT GGGTCCTCCA GAGCACAAAC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTC GAGGAATTTA TGACTAGGCA TCAAGTACAT
    GAAAAAGAAG AGTTCAAGGC CTTGAAAACA TTAAGTATTT TCTACCAAGC TGGGACTTCC
    AGAGCTGGGA ATCCTATATT TTATTATGTT GCACGGAGGT TCAAAACTGG TCAAATCAAT
    GGCGATTTGC TGATCTACCA TGTCTTGCTG ACTCTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACCGGG CCTAGCAATC GCTTTAAAAC GGACTTTCTC
    TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT GCTTATGACA ATGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAC ACCAAGTATC ACGAGCGGCT GCTCACTGGC
    CTCAAAGGCA GCAAAAGGCT CATTTTCATA GACTGTCCTG GGAAGCTGGC TGAGCACGTA
    GAGCACGAAC AACAGAAACT ACCTGCTGCC ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG
    TTCCACAATG CTCTCAAGCT GGCTCACAAA GACACCAAGG TTTCCATTAA AGTTGGTTCT
    ACTGCTGTTC AAGTGACCTC AGCAGAGCGA ACGAAAGTCC TAGGGCAATC AGTCTTTCTA
    AACGATATCT ACTATGCTTC GGAAATTGAA GAGATCTGCC TGGTGGATGA GAACCAGTTC
    ACCTTAACCA TTGCAAACCA GGGCACACCG CTCACCTTCA TGCACCAGGA GTGTGAAGCC
    ATCGTCCAGT CCATCATTCA CATCCGGACA CGCTGGGAGC TGTCACAGCC CGACTCCATC
    CCCCAGCACA CCAAGATCCG GCCAAAAGAT GTCCCTGGGA CACTGCTCAA TATCGCGTTA
    CTTAATTTAG GCAGTTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACTTGTA CCTTTAATTT AAAAATCGAG GGCCAGTTGC TAGAGACGTC AGGTTTATGT
    ATCCCTGCCA ACAACACCCT CTTTATTGTC TCTATTAGTA AGACACTGGC AGCCAATGAG
    CCACACCTCA CCTTAGAATT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAACTGAAAC ACCTTTGTTT GGAATACATG ACTCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCACA ATGATGATGC CAAAAGACAA AGAGTTACTG CTATTCTTGA TAAGCTGATA
    ACAATGACCA TAAATGAGAA ACAGATGTAC CCATCTATTC AAGCAAAAAT ATGGGGGAGC
    CTTGGGCAGA TTACAGATCT CCTTGATGTT GTGCTAGACA GTTTCATCAA AACCAGTGCA
    ACAGGTGGCT TGGGATCAAT AAAAGCCGAG GTGATGGCAG ATACTGCTGT AGCTCTGGCG
    TCTGGAAATG TGAAATTGGT TTCTAGCAAG GTTATTGGAA GGATGTGCAA AATAATTGAC
    AAGACATGCT TATCTCCAAC TCCTACTTTA GAACAGCATC TTATGTGGGA TGACATTGCG
    ATTCTAGCGC GCTACATGCT GATGTTGTCC TTCAACAACT CCCTCGACGT GGCGGCTCAT
    CTCCCCTACC TCTTCCACGT TGTGACTTTC TTAGTAGCCA CAGGGCCACT CTCCCTTAGA
    GCTTCCACAC ATGGATTGGT CATTAATATC ATTCACTCTC TGTGCACTTG TTCACAGCTT
    CATTTTAGTG AGGAGACCAA GCAAGTTTTG AGACTCAGTT TGACAGAGTT CTCATTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC
    TCCAGTTACC GGGACAGGTC GTTCTCTCCT GGCTCCTACG AGAGGGAGAC TTTTGCTTTG
    ACATCCTTGG AAACAGTCAC GGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGGTT TGCATTCCAG
    TACAACCCGT CCCTGCAACC GAGAGCTCTG GTCGTCTTTG GCTGCATTAG TAAGCGAGTG
    TCTCACGGGC AGATAAAGCA GATTATCCGC ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA
    AAAGGACCTG ATACTTACAA CAGTCAAGTT CTGATAGAAG CTACAGTAAT AGCACTAACC
    AAATTACAAC CACTTCTTAA TAAGGACTCC CCTCTGCACA AAGCCCTCTT TTGGGTAGCT
    GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC TTGTATTCAG CAGGCACCGC ACTTCTTGAG
    CAAAACCTGC ACACCTTAGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC AGAGGAAGTA
    TTTATGGCAA TTCGGAATCC TCTGGAATGG CACTGCAAGC AAATGGATCA TTTTGTTGGA
    CTCAATTTCA ACTCTAACTT TAACTTTGCA CTGGTTGGAC ACCTCTTAAA AGGGTACAGA
    CATCCTTCAC CTGCTATTGT TGCACGAACA GTCAGAATTT TGCATACACT ACTAACTCTG
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CGCAGAGCGT GGCTTACTTA
    GCAGCTTTAC TCACAGTATC TGAGGAGGTT CGAAGTCGCT GTAGCCTAAA ACATAGAAAG
    TCTCTTCTTC TTACTGATAT TTCAATGGAA AATATTCCTA TGGATACATA TCCCATTCAT
    CATGGCGACC CCAGCTATAG GACACTAAAG GAGAGTCAGC CATGGTCCTC TCCCAAAGGC
    TCGGAAGGGT ACCTTGCAGC CACCTATCCA ACCGTGGGCC AGACCAGCCC CCGAGCCCGG
    AAATCCATGA GCTTGGACAT GGGGCAGCCT TCTCAGGCCA ACACTAAGAA GTTGCTGGGA
    ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACGAAGG CTCCTAAAAG GCAAGAAGTG
    GAATCAGGGA TCACAACACC CCCCAAAATG AGGAGAGTAG CAGAAACTGA TTATGAAATG
    GAAACTCAAA GGATTTCCTC ATCACAACAG CACCCACATT TACGTAAAGT TTCAGTGTCT
    GAATCAAATG TTCTCTTGGA TGAAGAAGTA CTTACTGACC CGAAGATCCA GGCATTGCTG
    CTTACTGTTC TGGCTACACT GGTGAAATAC ACCACGGATG AGTTTGATCA ACGAATTCTT
    TATGAATACT TAGCAGAAGC CAGTGTTGTT TTCCCCAAAG TTTTTCCTGT TGTGCACAAT
    TTGTTGGACT CCAAGATCAA CACCCTGTTG TCATTGTGCC AAGATCCAAA TTTGTTAAAT
    CCGATTCATG GGATTGTGCA GAGTGTGGTA TACTACGAGG AGTCCCCACC ACAGTACCAG
    ACGTCTTACC TGCAGAGTTT TGGTTTTAAT GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA
    AAGCAAACAC AAATCCCAGA CTATGCTGAG CTTATTGTTA AGTTTCTTGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTATC CTCCTGCAGT GCAGAGCCAG CTTAGTATTA CTGCTAACCT TAACCTTTCC
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAACGTT
    GAACTCTCCC CAACCACTGG GCACTGTAAC AGTGGACGAA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb33975
    Clone ID Related Accession (Same CDS sequence) XM_014741967.1
    Accession Version XM_014741967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofibromin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867366.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATAATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCGAAGGACA CAATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCAGAAATC TGCCATTTTC TTCACGCCTG TCGTGAAGGA
    AACCAACATG CAGCTGAACT TCGGAATTCT GCCTCTGGGG TTTTATTTTC TCTCAGTTGC
    AACAACTTCA ATGCAGTCTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACTGTTTGT
    TCAGAAGATA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTCCT GAAGGAAACA GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT CGAAAATTAT
    CCAGATGAAT TTACAAAGCT ATACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCGCTTCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TCCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAGCAACAT GAATAAGAAG TTGTTTCTGG ACAGTCTGCG GAAAGCGCTT
    GCTGGCCATG GAGGAAGCAG GCAGCTGACT GAAAGTGCCG CCACTGCCTG TGTGAAGCTG
    TGCAAAGCGA GTACTTACAT CAACTGGGAA GACAACTCGG TCATTTTCCT ACTAGTTCAG
    TCCATGGTGG TCGATCTTAA GAACCTGCTT TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAACCTG CAGACGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGTATAAGC
    CCTCACAACA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCTCCTTC TACGTTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCACTGGA TTGGTGGCCT AAGATCGATG CCGTATATTG TCACTCAGTT
    GAACTTCGAA ATATGTTTGG TGAAACACTT CATAAAGCGG TGCAGGGTTG TGGAGCACAC
    CCGGCGATAC GCATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAGT ATCTTCTTTT GTCTATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CCAGAAAACA GGGGCCTGAA
    ACCCAAGGCA GTACAGCAGA ATTAATTACA GGGCTCGTCC AGCTGGTCCC TCAGTCACAC
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TGCTTCATCA GTTAGACAGC
    ATCGATTTGT GGAATCCTGA TGCTCCTGTG GAAACATTTT GGGAAATTAG CTCACAAATG
    CTTTTTTACA TCTGCAAGAA GTTAACTAGC CAGCAAATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGC GGGAAATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCA
    GATAGGAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCCAGT
    GGAAATACCA GTCAAATGTC CATGGATCAT GAAGAGTTAT TACGTACTTA TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCGGCAGG GTGCAGTGGA
    ACGCCGCCGA TATGCCGGCA AGCCCAGACC AAACTGGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACCGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGCCA CCTCTGTGAG
    GAAGCGGATA TCCGGTGTGG GGTGGACGAA GTATCGGTGC ACAACTTCTT GCCGAACTAT
    AACACATTCA TGGAATTTGC GTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTGAGGCGC ATTGAACATC CCACTGCAGG AAACACTGAG
    GCTTGGGAAG ATACACATGC AAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTATCCA
    AAAGCCAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ATAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTGAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTTCAG
    GAATGGATCA ACATGACCGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCCTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGCAAGGGT
    TCCATGATTT CAGTGATGTC CTCAGAGGGA AATGCAGACA CACCTGTCAG CAAATTTATG
    GATCGGCTGC TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGCACC
    AACGTTAAGG ATCTGGTGGG TTTAGAATTG AGTCCTGCTC TTTATCCAAT GCTGTTTAAC
    AAATTGAAGA ACACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AATTTGTAGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCCGAACA TCTGGGGCAA GCTAGCATTG AAACGATGAT GTTAAATCTG
    GTCAGATATG TTCGTGTACT TGGGAACATG GTCCATGCAA TTCAAATAAA AACGAAACTG
    TGTCAGCTAG TCGAGGTAAT GATGGCAAGA AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATATCTG ACAGATTGGG TTATGGGAAC ATCAAACCAA
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGATCTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTCCCGCTG CAGCCTGAGG AAGGAGATGG TGTGGAACTC
    ATGGAAGCCA AGTCACAGCT ATTTCTTAAG TATTTCACAT TGTTTATGAA CCTTTTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAGCG TGGCATGTCT
    CGGAGGCTGG CCTCACTGAG GCACTGCACA GTCCTTGCAA TGTCAAACTT ACTTAACGCT
    AACGTGGACA GTGGCCTCAT GCACTCCATA GGTTTAGGTT ATCACAAAGA TCTCCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCAGAAA CGGTGTTGGC AGATCGGTTT GAGAGGCTGG TGGAGTTGGT CACAATGATG
    GGTGATCAGG GAGAGCTCCC TATAGCCATG GCTCTGGCCA ATGTGGTCCC TTGTTCTCAG
    TGGGATGAAC TAGCTCGAGT TCTAGTGACT CTCTTTGATT CTCGGCATTT ACTCTACCAA
    CTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGCAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCCTGGA TCCTTTGTTA CGAATTGTGA TCACATCCTC TGATTGGCAA
    CATGTTAGCT TTGAAGTGGA TCCTACCAGG TTAGAACAGT CAGAGAGCCT TGAGGAGAAC
    CAGCGGAGCC TCCTTCAGAT GACTGAAAAG TTCTTCCACG CCATCATCAG TTCCTCCTCG
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA CAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTCATCAAT
    CCTGCCATCG TCTCACCGTA TGAAGCAGGC ATTTTAGATA AAAAGCCACC ACCTAGAATC
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTCTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AAAGCAACTT TGATGCAGCA
    CGAAGGTTTT TCCTTGATAT AGCATCCGAT TGTCCTACAA GTGATGCAGT GAATCACAGT
    CTTTCCTTCA TCAGCGATGG CAACGTGCTG GCTCTACATC GTCTCCTCTG GAACAATCAA
    GAGAAGATTG GCCAGTATCT TTCCAGCAAT AGGGATCATA AAGCTGTTGG AAGACGACCT
    TTTGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TCGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCCTTGAAAA CATTAAGTAT TTTCTACCAA
    GCTGGGACTT CCAGAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTCAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATCTAC CATGTCTTGC TGACTCTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACCCATACCG GGCCTAGCAA TCGCTTTAAA
    ACGGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TTGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGGGAGT ACACCAAGTA TCACGAGCGG
    CTGCTCACTG GCCTCAAAGG CAGCAAAAGG CTCATTTTCA TAGACTGTCC TGGGAAGCTG
    GCTGAGCACG TAGAGCACGA ACAACAGAAA CTACCTGCTG CCACTTTGGC TTTAGAAGAG
    GACCTGAAAG TGTTCCACAA TGCTCTCAAG CTGGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTGACC TCAGCAGAGC GAACGAAAGT CCTAGGGCAA
    TCAGTCTTTC TAAACGATAT CTACTATGCT TCGGAAATTG AAGAGATCTG CCTGGTGGAT
    GAGAACCAGT TCACCTTAAC CATTGCAAAC CAGGGCACAC CGCTCACCTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA GTCCATCATT CACATCCGGA CACGCTGGGA GCTGTCACAG
    CCCGACTCCA TCCCCCAGCA CACCAAGATC CGGCCAAAAG ATGTCCCTGG GACACTGCTC
    AATATCGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTTAAT TTAAAAATCG AGGGCCAGTT GCTAGAGACG
    TCAGGTTTAT GTATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACACCT CACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAATCTAGTA TTGAACTGAA ACACCTTTGT TTGGAATACA TGACTCCATG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AAAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT TCAAGCAAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTGCTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCCG AGGTGATGGC AGATACTGCT
    GTAGCTCTGG CGTCTGGAAA TGTGAAATTG GTTTCTAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG CTTATCTCCA ACTCCTACTT TAGAACAGCA TCTTATGTGG
    GATGACATTG CGATTCTAGC GCGCTACATG CTGATGTTGT CCTTCAACAA CTCCCTCGAC
    GTGGCGGCTC ATCTCCCCTA CCTCTTCCAC GTTGTGACTT TCTTAGTAGC CACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGCACT
    TGTTCACAGC TTCATTTTAG TGAGGAGACC AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAATTTTA CTTACTGTTT GGCATTAGCA AAGTCAAGTC AGCTGCTGTG
    ATTGCCTTCC GCTCCAGTTA CCGGGACAGG TCGTTCTCTC CTGGCTCCTA CGAGAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACGGAAGCTT TGTTGGAGAT CATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGATCAGT GGACAGAACT AGCTCAAAGG
    TTTGCATTCC AGTACAACCC GTCCCTGCAA CCGAGAGCTC TGGTCGTCTT TGGCTGCATT
    AGTAAGCGAG TGTCTCACGG GCAGATAAAG CAGATTATCC GCATTCTTAG CAAGGCGCTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTAG CTGTGGCCGT GCTGCAGCTC GATGAGGTCA ACTTGTATTC AGCAGGCACC
    GCACTTCTTG AGCAAAACCT GCACACCTTA GACAGTCTCC GGATATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATTCGGAAT CCTCTGGAAT GGCACTGCAA GCAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTTG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GACATCCTTC ACCTGCTATT GTTGCACGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTTAACAA ACATAGAAAT TGTGACAAAT TTGAGGTGAA TACGCAGAGC
    GTGGCTTACT TAGCAGCTTT ACTCACAGTA TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AGTCTCTTCT TCTTACTGAT ATTTCAATGG AAAATATTCC TATGGATACA
    TATCCCATTC ATCATGGCGA CCCCAGCTAT AGGACACTAA AGGAGAGTCA GCCATGGTCC
    TCTCCCAAAG GCTCGGAAGG GTACCTTGCA GCCACCTATC CAACCGTGGG CCAGACCAGC
    CCCCGAGCCC GGAAATCCAT GAGCTTGGAC ATGGGGCAGC CTTCTCAGGC CAACACTAAG
    AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACGAA GGCTCCTAAA
    AGGCAAGAAG TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT
    GATTATGAAA TGGAAACTCA AAGGATTTCC TCATCACAAC AGCACCCACA TTTACGTAAA
    GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA CCCGAAGATC
    CAGGCATTGC TGCTTACTGT TCTGGCTACA CTGGTGAAAT ACACCACGGA TGAGTTTGAT
    CAACGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA AGTTTTTCCT
    GTTGTGCACA ATTTGTTGGA CTCCAAGATC AACACCCTGT TGTCATTGTG CCAAGATCCA
    AATTTGTTAA ATCCGATTCA TGGGATTGTG CAGAGTGTGG TATACTACGA GGAGTCCCCA
    CCACAGTACC AGACGTCTTA CCTGCAGAGT TTTGGTTTTA ATGGCTTGTG GCGGTTTGCA
    GGACCGTTTT CAAAGCAAAC ACAAATCCCA GACTATGCTG AGCTTATTGT TAAGTTTCTT
    GATGCCTTGA TTGACACGTA TTTGCCTGGA ATTGATGAAG AAACCAGTGA GGAGTCCCTC
    CTGACACCCA CATCTCCTTA TCCTCCTGCA GTGCAGAGCC AGCTTAGTAT TACTGCTAAC
    CTTAACCTTT CCAATTCCAT GACCTCACTT GCAACTTCCC AGCATTCCCC AGCTTCTCTG
    CCTTGCTCTA AATCAGCAGT TTCCATGCAG CTGTTCCCTC ATCAAGGAAT CGACAAGGAG
    AACGTTGAAC TCTCCCCAAC CACTGGGCAC TGTAACAGTG GACGAACTCG CCATGGATCC
    GCAAGCCAAG TGCAGAAGCA AAGAAGCGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG
    ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014597453.1
    CDS201..8609
    Translation

    Target ORF information:

    RefSeq Version XM_014741967.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014741967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGAGAATAT TTGGAGAAGC TGCTGAAAAA AATTTATATC TCTCTCAGTT GATAATATTG 
    GATACACTGG AAAAATGTCT TGCTGGGCAA CCGAAGGACA CAATGAGATT AGATGAAACA
    ATGCTGGTCA AACAGTTGCT GCCAGAAATC TGCCATTTTC TTCACGCCTG TCGTGAAGGA
    AACCAACATG CAGCTGAACT TCGGAATTCT GCCTCTGGGG TTTTATTTTC TCTCAGTTGC
    AACAACTTCA ATGCAGTCTT TAGTCGCATT TCTACCAGGT TACAGGAATT GACTGTTTGT
    TCAGAAGATA ATGTTGATGT TCATGATATA GAATTGTTAC AGTATATCAA TGTGGATTGT
    GCAAAATTAA AACGACTCCT GAAGGAAACA GCATTTAAAT TTAAAGCCCT AAAGAAGGTT
    GCACAGTTAG CAGTTATAAA TAGCCTGGAA AAAGCATTTT GGAACTGGGT CGAAAATTAT
    CCAGATGAAT TTACAAAGCT ATACCAGATT CCACAGACTG ATATGGCTGA GTGTGCGGAA
    AAACTGTTTG ACTTGGTGGA TGGTTTTGCT GAAAGCACCA AACGTAAAGC AGCAGTTTGG
    CCGCTTCAAA TCATTCTCCT TATCTTGTGT CCAGAAATAA TCCAGGATAT ATCCAAGGAT
    GTGGTTGATG AAAGCAACAT GAATAAGAAG TTGTTTCTGG ACAGTCTGCG GAAAGCGCTT
    GCTGGCCATG GAGGAAGCAG GCAGCTGACT GAAAGTGCCG CCACTGCCTG TGTGAAGCTG
    TGCAAAGCGA GTACTTACAT CAACTGGGAA GACAACTCGG TCATTTTCCT ACTAGTTCAG
    TCCATGGTGG TCGATCTTAA GAACCTGCTT TTTAATCCAA GTAAACCATT CTCAAGAGGC
    AGTCAACCTG CAGACGTGGA TCTAATGATT GACTGCCTTG TTTCTTGCTT TCGTATAAGC
    CCTCACAACA ACCAACACTT TAAGATCTGC CTGGCTCAGA ATTCTCCTTC TACGTTTCAC
    TATGTGCTGG TAAATTCACT CCATCGAATC ATCACCAATA GCACTTTCAA GCATGGACTT
    GGCACTGCTT CTGCACTGGA TTGGTGGCCT AAGATCGATG CCGTATATTG TCACTCAGTT
    GAACTTCGAA ATATGTTTGG TGAAACACTT CATAAAGCGG TGCAGGGTTG TGGAGCACAC
    CCGGCGATAC GCATGGCACC GAGTCTTACA TTTAAAGAAA AAGTAACAAG CCTTAAATTT
    AAAGAAAAAC CTACAGACCT GGAGACAAGA AGCTATAAGT ATCTTCTTTT GTCTATGGTG
    AAACTAATTC ATGCAGATCC AAAGCTCTTG CTTTGTAATC CCAGAAAACA GGGGCCTGAA
    ACCCAAGGCA GTACAGCAGA ATTAATTACA GGGCTCGTCC AGCTGGTCCC TCAGTCACAC
    ATGCCAGAGA TTGCTCAGGA AGCAATGGAG GCTCTGCTGG TGCTTCATCA GTTAGACAGC
    ATCGATTTGT GGAATCCTGA TGCTCCTGTG GAAACATTTT GGGAAATTAG CTCACAAATG
    CTTTTTTACA TCTGCAAGAA GTTAACTAGC CAGCAAATGC TTAGTAGCAC AGAAATTCTC
    AAATGGTTGC GGGAAATTTT GATCTGCAGG AATAAATTTC TTCTTAAAAA TAAGCAGGCA
    GATAGGAGTT CCTGTCACTT TCTCTTCCTT TATGGGGTAG GATGTGATGT TCCTTCCAGT
    GGAAATACCA GTCAAATGTC CATGGATCAT GAAGAGTTAT TACGTACTTA TACTCCTGGA
    GCCTCTCTCC GGAAGGGAAA AGGAAACTCA TCCATGGATA GTGCGGCAGG GTGCAGTGGA
    ACGCCGCCGA TATGCCGGCA AGCCCAGACC AAACTGGAAG TGGCCCTGTA CATGTTTCTG
    TGGAGCCCTG ACACCGAAGC TGTTCTGGTT GCCATGTCCT GTTTCCGCCA CCTCTGTGAG
    GAAGCGGATA TCCGGTGTGG GGTGGACGAA GTATCGGTGC ACAACTTCTT GCCGAACTAT
    AACACATTCA TGGAATTTGC GTCTGTCAGC AATATGATGT CAACAGGAAG AGCAGCACTT
    CAGAAAAGAG TGATGGCACT ACTGAGGCGC ATTGAACATC CCACTGCAGG AAACACTGAG
    GCTTGGGAAG ATACACATGC AAAGTGGGAA CAAGCTACAA AACTAATCCT CAACTATCCA
    AAAGCCAAAA TGGAAGATGG CCAGGCTGCT GAAAGCCTTC ATAAGACCAT TGTTAAGAGG
    CGAATGTCTC ATGTGAGTGG AGGAGGATCC ATAGATTTGT CTGACACGGA CTCCCTTCAG
    GAATGGATCA ACATGACCGG CTTCCTCTGT GCCCTTGGGG GAGTGTGCCT GCAGCAGAGA
    AGCAATTCTG GCCTGGCAAC CTATAGCCCA CCCATGGGCC CAGTCAGTGA ACGCAAGGGT
    TCCATGATTT CAGTGATGTC CTCAGAGGGA AATGCAGACA CACCTGTCAG CAAATTTATG
    GATCGGCTGC TATCCTTAAT GGTGTGTAAC CATGAGAAAG TGGGACTTCA AATACGCACC
    AACGTTAAGG ATCTGGTGGG TTTAGAATTG AGTCCTGCTC TTTATCCAAT GCTGTTTAAC
    AAATTGAAGA ACACCATCAG CAAGTTTTTT GACTCCCAAG GACAGGTTTT ATTGACTGAC
    ACCAATACTC AATTTGTAGA GCAAACCATA GCTATAATGA AGAACTTACT AGATAATCAT
    ACTGAAGGCA GCTCCGAACA TCTGGGGCAA GCTAGCATTG AAACGATGAT GTTAAATCTG
    GTCAGATATG TTCGTGTACT TGGGAACATG GTCCATGCAA TTCAAATAAA AACGAAACTG
    TGTCAGCTAG TCGAGGTAAT GATGGCAAGA AGAGATGACC TCTCATTTTG TCAAGAGATG
    AAATTTAGGA ATAAGATGGT AGAATATCTG ACAGATTGGG TTATGGGAAC ATCAAACCAA
    GCAGCAGATG ATGATGTAAA ATGTCTTACA AGAGATCTGG ACCAGGCAAG CATGGAAGCA
    GTAGTTTCAC TCCTGGCTGG TCTCCCGCTG CAGCCTGAGG AAGGAGATGG TGTGGAACTC
    ATGGAAGCCA AGTCACAGCT ATTTCTTAAG TATTTCACAT TGTTTATGAA CCTTTTGAAT
    GACTGTAGTG AAGTTGAAGA TGAAAATGCA CAAACAGGTG GCAGGAAGCG TGGCATGTCT
    CGGAGGCTGG CCTCACTGAG GCACTGCACA GTCCTTGCAA TGTCAAACTT ACTTAACGCT
    AACGTGGACA GTGGCCTCAT GCACTCCATA GGTTTAGGTT ATCACAAAGA TCTCCAGACA
    AGAGCTACAT TTATGGAAGT TCTGACAAAA ATCCTCCAAC AAGGCACAGA ATTTGACACA
    CTTGCAGAAA CGGTGTTGGC AGATCGGTTT GAGAGGCTGG TGGAGTTGGT CACAATGATG
    GGTGATCAGG GAGAGCTCCC TATAGCCATG GCTCTGGCCA ATGTGGTCCC TTGTTCTCAG
    TGGGATGAAC TAGCTCGAGT TCTAGTGACT CTCTTTGATT CTCGGCATTT ACTCTACCAA
    CTGCTCTGGA ACATGTTTTC TAAGGAAGTA GAATTGGCAG ACTCCATGCA GACTCTTTTC
    CGAGGCAACA GCTTAGCCAG TAAAATAATG ACCTTCTGTT TCAAGGTATA TGGTGCTACC
    TACCTACAAA AACTCCTGGA TCCTTTGTTA CGAATTGTGA TCACATCCTC TGATTGGCAA
    CATGTTAGCT TTGAAGTGGA TCCTACCAGG TTAGAACAGT CAGAGAGCCT TGAGGAGAAC
    CAGCGGAGCC TCCTTCAGAT GACTGAAAAG TTCTTCCACG CCATCATCAG TTCCTCCTCG
    GAATTCCCCC CTCAGCTTCG AAGTGTGTGC CACTGTTTAT ACCAGGCAAC TTGCCACTCC
    CTACTGAATA CAGCTACAGT AAAAGAAAAA AAGGAAAACA AAAAATCAGT GGTTAGCCAG
    CGTTTCCCTC AGAACAGCAT CGGTGCAGTG GGAAGTGCCA TGTTCCTCAG ATTCATCAAT
    CCTGCCATCG TCTCACCGTA TGAAGCAGGC ATTTTAGATA AAAAGCCACC ACCTAGAATC
    GAAAGGGGCT TGAAGTTAAT GTCAAAGATA CTTCAGAGTA TTGCCAATCA TGTTCTCTTC
    ACAAAAGAAG AGCATATGCG GCCTTTTAAT GATTTTGTGA AAAGCAACTT TGATGCAGCA
    CGAAGGTTTT TCCTTGATAT AGCATCCGAT TGTCCTACAA GTGATGCAGT GAATCACAGT
    CTTTCCTTCA TCAGCGATGG CAACGTGCTG GCTCTACATC GTCTCCTCTG GAACAATCAA
    GAGAAGATTG GCCAGTATCT TTCCAGCAAT AGGGATCATA AAGCTGTTGG AAGACGACCT
    TTTGATAAGA TGGCAACACT TCTTGCATAC CTGGGTCCTC CAGAGCACAA ACCTGTGGCA
    GATACACACT GGTCCAGCCT TAACCTTACC AGTTCAAAGT TCGAGGAATT TATGACTAGG
    CATCAAGTAC ATGAAAAAGA AGAGTTCAAG GCCTTGAAAA CATTAAGTAT TTTCTACCAA
    GCTGGGACTT CCAGAGCTGG GAATCCTATA TTTTATTATG TTGCACGGAG GTTCAAAACT
    GGTCAAATCA ATGGCGATTT GCTGATCTAC CATGTCTTGC TGACTCTAAA GCCATATTAT
    GCAAAGCCAT ATGAAATTGT AGTGGACCTT ACCCATACCG GGCCTAGCAA TCGCTTTAAA
    ACGGACTTTC TCTCCAAGTG GTTTGTTGTT TTTCCTGGCT TTGCTTATGA CAATGTCTCT
    GCAGTCTATA TCTATAACTG TAACTCCTGG GTCAGGGAGT ACACCAAGTA TCACGAGCGG
    CTGCTCACTG GCCTCAAAGG CAGCAAAAGG CTCATTTTCA TAGACTGTCC TGGGAAGCTG
    GCTGAGCACG TAGAGCACGA ACAACAGAAA CTACCTGCTG CCACTTTGGC TTTAGAAGAG
    GACCTGAAAG TGTTCCACAA TGCTCTCAAG CTGGCTCACA AAGACACCAA GGTTTCCATT
    AAAGTTGGTT CTACTGCTGT TCAAGTGACC TCAGCAGAGC GAACGAAAGT CCTAGGGCAA
    TCAGTCTTTC TAAACGATAT CTACTATGCT TCGGAAATTG AAGAGATCTG CCTGGTGGAT
    GAGAACCAGT TCACCTTAAC CATTGCAAAC CAGGGCACAC CGCTCACCTT CATGCACCAG
    GAGTGTGAAG CCATCGTCCA GTCCATCATT CACATCCGGA CACGCTGGGA GCTGTCACAG
    CCCGACTCCA TCCCCCAGCA CACCAAGATC CGGCCAAAAG ATGTCCCTGG GACACTGCTC
    AATATCGCGT TACTTAATTT AGGCAGTTCA GACCCTAGTT TACGGTCAGC TGCCTATAAT
    CTTCTGTGTG CCTTAACTTG TACCTTTAAT TTAAAAATCG AGGGCCAGTT GCTAGAGACG
    TCAGGTTTAT GTATCCCTGC CAACAACACC CTCTTTATTG TCTCTATTAG TAAGACACTG
    GCAGCCAATG AGCCACACCT CACCTTAGAA TTTTTGGAAG AGTGTATTTC TGGATTTAGC
    AAATCTAGTA TTGAACTGAA ACACCTTTGT TTGGAATACA TGACTCCATG GCTGTCAAAT
    CTAGTCCGTT TTTGTAAGCA CAATGATGAT GCCAAAAGAC AAAGAGTTAC TGCTATTCTT
    GATAAGCTGA TAACAATGAC CATAAATGAG AAACAGATGT ACCCATCTAT TCAAGCAAAA
    ATATGGGGGA GCCTTGGGCA GATTACAGAT CTCCTTGATG TTGTGCTAGA CAGTTTCATC
    AAAACCAGTG CAACAGGTGG CTTGGGATCA ATAAAAGCCG AGGTGATGGC AGATACTGCT
    GTAGCTCTGG CGTCTGGAAA TGTGAAATTG GTTTCTAGCA AGGTTATTGG AAGGATGTGC
    AAAATAATTG ACAAGACATG CTTATCTCCA ACTCCTACTT TAGAACAGCA TCTTATGTGG
    GATGACATTG CGATTCTAGC GCGCTACATG CTGATGTTGT CCTTCAACAA CTCCCTCGAC
    GTGGCGGCTC ATCTCCCCTA CCTCTTCCAC GTTGTGACTT TCTTAGTAGC CACAGGGCCA
    CTCTCCCTTA GAGCTTCCAC ACATGGATTG GTCATTAATA TCATTCACTC TCTGTGCACT
    TGTTCACAGC TTCATTTTAG TGAGGAGACC AAGCAAGTTT TGAGACTCAG TTTGACAGAG
    TTCTCATTAC CCAAATTTTA CTTACTGTTT GGCATTAGCA AAGTCAAGTC AGCTGCTGTG
    ATTGCCTTCC GCTCCAGTTA CCGGGACAGG TCGTTCTCTC CTGGCTCCTA CGAGAGGGAG
    ACTTTTGCTT TGACATCCTT GGAAACAGTC ACGGAAGCTT TGTTGGAGAT CATGGAGGCA
    TGTATGAGAG ATATTCCAAC ATGCAAGTGG CTGGATCAGT GGACAGAACT AGCTCAAAGG
    TTTGCATTCC AGTACAACCC GTCCCTGCAA CCGAGAGCTC TGGTCGTCTT TGGCTGCATT
    AGTAAGCGAG TGTCTCACGG GCAGATAAAG CAGATTATCC GCATTCTTAG CAAGGCGCTT
    GAGAGTTGCT TAAAAGGACC TGATACTTAC AACAGTCAAG TTCTGATAGA AGCTACAGTA
    ATAGCACTAA CCAAATTACA ACCACTTCTT AATAAGGACT CCCCTCTGCA CAAAGCCCTC
    TTTTGGGTAG CTGTGGCCGT GCTGCAGCTC GATGAGGTCA ACTTGTATTC AGCAGGCACC
    GCACTTCTTG AGCAAAACCT GCACACCTTA GACAGTCTCC GGATATTCAA TGACAAGAGT
    CCAGAGGAAG TATTTATGGC AATTCGGAAT CCTCTGGAAT GGCACTGCAA GCAAATGGAT
    CATTTTGTTG GACTCAATTT CAACTCTAAC TTTAACTTTG CACTGGTTGG ACACCTCTTA
    AAAGGGTACA GACATCCTTC ACCTGCTATT GTTGCACGAA CAGTCAGAAT TTTGCATACA
    CTACTAACTC TGGTTAACAA ACATAGAAAT TGTGACAAAT TTGAGGTGAA TACGCAGAGC
    GTGGCTTACT TAGCAGCTTT ACTCACAGTA TCTGAGGAGG TTCGAAGTCG CTGTAGCCTA
    AAACATAGAA AGTCTCTTCT TCTTACTGAT ATTTCAATGG AAAATATTCC TATGGATACA
    TATCCCATTC ATCATGGCGA CCCCAGCTAT AGGACACTAA AGGAGAGTCA GCCATGGTCC
    TCTCCCAAAG GCTCGGAAGG GTACCTTGCA GCCACCTATC CAACCGTGGG CCAGACCAGC
    CCCCGAGCCC GGAAATCCAT GAGCTTGGAC ATGGGGCAGC CTTCTCAGGC CAACACTAAG
    AAGTTGCTGG GAACAAGGAA AAGTTTTGAT CACTTGATAT CAGACACGAA GGCTCCTAAA
    AGGCAAGAAG TGGAATCAGG GATCACAACA CCCCCCAAAA TGAGGAGAGT AGCAGAAACT
    GATTATGAAA TGGAAACTCA AAGGATTTCC TCATCACAAC AGCACCCACA TTTACGTAAA
    GTTTCAGTGT CTGAATCAAA TGTTCTCTTG GATGAAGAAG TACTTACTGA CCCGAAGATC
    CAGGCATTGC TGCTTACTGT TCTGGCTACA CTGGTGAAAT ACACCACGGA TGAGTTTGAT
    CAACGAATTC TTTATGAATA CTTAGCAGAA GCCAGTGTTG TTTTCCCCAA AGTTTTTCCT
    GTTGTGCACA ATTTGTTGGA CTCCAAGATC AACACCCTGT TGTCATTGTG CCAAGATCCA
    AATTTGTTAA ATCCGATTCA TGGGATTGTG CAGAGTGTGG TATACTACGA GGAGTCCCCA
    CCACAGTACC AGACGTCTTA CCTGCAGAGT TTTGGTTTTA ATGGCTTGTG GCGGTTTGCA
    GGACCGTTTT CAAAGCAAAC ACAAATCCCA GACTATGCTG AGCTTATTGT TAAGTTTCTT
    GATGCCTTGA TTGACACGTA TTTGCCTGGA ATTGATGAAG AAACCAGTGA GGAGTCCCTC
    CTGACACCCA CATCTCCTTA TCCTCCTGCA GTGCAGAGCC AGCTTAGTAT TACTGCTAAC
    CTTAACCTTT CCAATTCCAT GACCTCACTT GCAACTTCCC AGCATTCCCC AGCTTCTCTG
    CCTTGCTCTA AATCAGCAGT TTCCATGCAG CTGTTCCCTC ATCAAGGAAT CGACAAGGAG
    AACGTTGAAC TCTCCCCAAC CACTGGGCAC TGTAACAGTG GACGAACTCG CCATGGATCC
    GCAAGCCAAG TGCAGAAGCA AAGAAGCGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG
    ATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45484
    Clone ID Related Accession (Same CDS sequence) XM_023653055.1
    Accession Version XM_023653055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGCAA CTTGCCACTC CCTACTGAAT ACAGCTACAG TAAAAGAAAA AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGTTTCCCT CAGAACAGCA TCGGTGCAGT GGGAAGTGCC
    ATGTTCCTCA GATTCATCAA TCCTGCCATC GTCTCACCGT ATGAAGCAGG CATTTTAGAT
    AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT
    ATTGCCAATC ATGTTCTCTT CACAAAAGAA GAGCATATGC GGCCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGATGCAGC ACGAAGGTTT TTCCTTGATA TAGCATCCGA TTGTCCTACA
    AGTGATGCAG TGAATCACAG TCTTTCCTTC ATCAGCGATG GCAACGTGCT GGCTCTACAT
    CGTCTCCTCT GGAACAATCA AGAGAAGATT GGCCAGTATC TTTCCAGCAA TAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCAACAC TTCTTGCATA CCTGGGTCCT
    CCAGAGCACA AACCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTCGAGGAAT TTATGACTAG GCATCAAGTA CATGAAAAAG AAGAGTTCAA GGCCTTGAAA
    ACATTAAGTA TTTTCTACCA AGCTGGGACT TCCAGAGCTG GGAATCCTAT ATTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGTCAAATC AATGGCGATT TGCTGATCTA CCATGTCTTG
    CTGACTCTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACC
    GGGCCTAGCA ATCGCTTTAA AACGGACTTT CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC
    TTTGCTTATG ACAATGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TACACCAAGT ATCACGAGCG GCTGCTCACT GGCCTCAAAG GCAGCAAAAG GCTCATTTTC
    ATAGACTGTC CTGGGAAGCT GGCTGAGCAC GTAGAGCACG AACAACAGAA ACTACCTGCT
    GCCACTTTGG CTTTAGAAGA GGACCTGAAA GTGTTCCACA ATGCTCTCAA GCTGGCTCAC
    AAAGACACCA AGGTTTCCAT TAAAGTTGGT TCTACTGCTG TTCAAGTGAC CTCAGCAGAG
    CGAACGAAAG TCCTAGGGCA ATCAGTCTTT CTAAACGATA TCTACTATGC TTCGGAAATT
    GAAGAGATCT GCCTGGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAGGGCACA
    CCGCTCACCT TCATGCACCA GGAGTGTGAA GCCATCGTCC AGTCCATCAT TCACATCCGG
    ACACGCTGGG AGCTGTCACA GCCCGACTCC ATCCCCCAGC ACACCAAGAT CCGGCCAAAA
    GATGTCCCTG GGACACTGCT CAATATCGCG TTACTTAATT TAGGCAGTTC AGACCCTAGT
    TTACGGTCAG CTGCCTATAA TCTTCTGTGT GCCTTAACTT GTACCTTTAA TTTAAAAATC
    GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA TGTATCCCTG CCAACAACAC CCTCTTTATT
    GTCTCTATTA GTAAGACACT GGCAGCCAAT GAGCCACACC TCACCTTAGA ATTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAACTGA AACACCTTTG TTTGGAATAC
    ATGACTCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ACAATGATGA TGCCAAAAGA
    CAAAGAGTTA CTGCTATTCT TGATAAGCTG ATAACAATGA CCATAAATGA GAAACAGATG
    TACCCATCTA TTCAAGCAAA AATATGGGGG AGCCTTGGGC AGATTACAGA TCTCCTTGAT
    GTTGTGCTAG ACAGTTTCAT CAAAACCAGT GCAACAGGTG GCTTGGGATC AATAAAAGCC
    GAGGTGATGG CAGATACTGC TGTAGCTCTG GCGTCTGGAA ATGTGAAATT GGTTTCTAGC
    AAGGTTATTG GAAGGATGTG CAAAATAATT GACAAGACAT GCTTATCTCC AACTCCTACT
    TTAGAACAGC ATCTTATGTG GGATGACATT GCGATTCTAG CGCGCTACAT GCTGATGTTG
    TCCTTCAACA ACTCCCTCGA CGTGGCGGCT CATCTCCCCT ACCTCTTCCA CGTTGTGACT
    TTCTTAGTAG CCACAGGGCC ACTCTCCCTT AGAGCTTCCA CACATGGATT GGTCATTAAT
    ATCATTCACT CTCTGTGCAC TTGTTCACAG CTTCATTTTA GTGAGGAGAC CAAGCAAGTT
    TTGAGACTCA GTTTGACAGA GTTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CAGCTGCTGT GATTGCCTTC CGCTCCAGTT ACCGGGACAG GTCGTTCTCT
    CCTGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCT TGGAAACAGT CACGGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG GTTTGCATTC CAGTACAACC CGTCCCTGCA ACCGAGAGCT
    CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA GTGTCTCACG GGCAGATAAA GCAGATTATC
    CGCATTCTTA GCAAGGCGCT TGAGAGTTGC TTAAAAGGAC CTGATACTTA CAACAGTCAA
    GTTCTGATAG AAGCTACAGT AATAGCACTA ACCAAATTAC AACCACTTCT TAATAAGGAC
    TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA GCTGTGGCCG TGCTGCAGCT CGATGAGGTC
    AACTTGTATT CAGCAGGCAC CGCACTTCTT GAGCAAAACC TGCACACCTT AGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCAGAGGAA GTATTTATGG CAATTCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAAATGGA TCATTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT
    GCACTGGTTG GACACCTCTT AAAAGGGTAC AGACATCCTT CACCTGCTAT TGTTGCACGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTGGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACGCAGAG CGTGGCTTAC TTAGCAGCTT TACTCACAGT ATCTGAGGAG
    GTTCGAAGTC GCTGTAGCCT AAAACATAGA AAGTCTCTTC TTCTTACTGA TATTTCAATG
    GAAAATATTC CTATGGATAC ATATCCCATT CATCATGGCG ACCCCAGCTA TAGGACACTA
    AAGGAGAGTC AGCCATGGTC CTCTCCCAAA GGCTCGGAAG GGTACCTTGC AGCCACCTAT
    CCAACCGTGG GCCAGACCAG CCCCCGAGCC CGGAAATCCA TGAGCTTGGA CATGGGGCAG
    CCTTCTCAGG CCAACACTAA GAAGTTGCTG GGAACAAGGA AAAGTTTTGA TCACTTGATA
    TCAGACACGA AGGCTCCTAA AAGGCAAGAA GTGGAATCAG GGATCACAAC ACCCCCCAAA
    ATGAGGAGAG TAGCAGAAAC TGATTATGAA ATGGAAACTC AAAGGATTTC CTCATCACAA
    CAGCACCCAC ATTTACGTAA AGTTTCAGTG TCTGAATCAA ATGTTCTCTT GGATGAAGAA
    GTACTTACTG ACCCGAAGAT CCAGGCATTG CTGCTTACTG TTCTGGCTAC ACTGGTGAAA
    TACACCACGG ATGAGTTTGA TCAACGAATT CTTTATGAAT ACTTAGCAGA AGCCAGTGTT
    GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC AATTTGTTGG ACTCCAAGAT CAACACCCTG
    TTGTCATTGT GCCAAGATCC AAATTTGTTA AATCCGATTC ATGGGATTGT GCAGAGTGTG
    GTATACTACG AGGAGTCCCC ACCACAGTAC CAGACGTCTT ACCTGCAGAG TTTTGGTTTT
    AATGGCTTGT GGCGGTTTGC AGGACCGTTT TCAAAGCAAA CACAAATCCC AGACTATGCT
    GAGCTTATTG TTAAGTTTCT TGATGCCTTG ATTGACACGT ATTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ATCCTCCTGC AGTGCAGAGC
    CAGCTTAGTA TTACTGCTAA CCTTAACCTT TCCAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGAATCGA CAAGGAGAAC GTTGAACTCT CCCCAACCAC TGGGCACTGT
    AACAGTGGAC GAACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGCGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508823.1
    CDS165..8720
    Translation

    Target ORF information:

    RefSeq Version XM_023653055.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGCAA CTTGCCACTC CCTACTGAAT ACAGCTACAG TAAAAGAAAA AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGTTTCCCT CAGAACAGCA TCGGTGCAGT GGGAAGTGCC
    ATGTTCCTCA GATTCATCAA TCCTGCCATC GTCTCACCGT ATGAAGCAGG CATTTTAGAT
    AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT
    ATTGCCAATC ATGTTCTCTT CACAAAAGAA GAGCATATGC GGCCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGATGCAGC ACGAAGGTTT TTCCTTGATA TAGCATCCGA TTGTCCTACA
    AGTGATGCAG TGAATCACAG TCTTTCCTTC ATCAGCGATG GCAACGTGCT GGCTCTACAT
    CGTCTCCTCT GGAACAATCA AGAGAAGATT GGCCAGTATC TTTCCAGCAA TAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCAACAC TTCTTGCATA CCTGGGTCCT
    CCAGAGCACA AACCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTCGAGGAAT TTATGACTAG GCATCAAGTA CATGAAAAAG AAGAGTTCAA GGCCTTGAAA
    ACATTAAGTA TTTTCTACCA AGCTGGGACT TCCAGAGCTG GGAATCCTAT ATTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGTCAAATC AATGGCGATT TGCTGATCTA CCATGTCTTG
    CTGACTCTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACC
    GGGCCTAGCA ATCGCTTTAA AACGGACTTT CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC
    TTTGCTTATG ACAATGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TACACCAAGT ATCACGAGCG GCTGCTCACT GGCCTCAAAG GCAGCAAAAG GCTCATTTTC
    ATAGACTGTC CTGGGAAGCT GGCTGAGCAC GTAGAGCACG AACAACAGAA ACTACCTGCT
    GCCACTTTGG CTTTAGAAGA GGACCTGAAA GTGTTCCACA ATGCTCTCAA GCTGGCTCAC
    AAAGACACCA AGGTTTCCAT TAAAGTTGGT TCTACTGCTG TTCAAGTGAC CTCAGCAGAG
    CGAACGAAAG TCCTAGGGCA ATCAGTCTTT CTAAACGATA TCTACTATGC TTCGGAAATT
    GAAGAGATCT GCCTGGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAGGGCACA
    CCGCTCACCT TCATGCACCA GGAGTGTGAA GCCATCGTCC AGTCCATCAT TCACATCCGG
    ACACGCTGGG AGCTGTCACA GCCCGACTCC ATCCCCCAGC ACACCAAGAT CCGGCCAAAA
    GATGTCCCTG GGACACTGCT CAATATCGCG TTACTTAATT TAGGCAGTTC AGACCCTAGT
    TTACGGTCAG CTGCCTATAA TCTTCTGTGT GCCTTAACTT GTACCTTTAA TTTAAAAATC
    GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA TGTATCCCTG CCAACAACAC CCTCTTTATT
    GTCTCTATTA GTAAGACACT GGCAGCCAAT GAGCCACACC TCACCTTAGA ATTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAACTGA AACACCTTTG TTTGGAATAC
    ATGACTCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ACAATGATGA TGCCAAAAGA
    CAAAGAGTTA CTGCTATTCT TGATAAGCTG ATAACAATGA CCATAAATGA GAAACAGATG
    TACCCATCTA TTCAAGCAAA AATATGGGGG AGCCTTGGGC AGATTACAGA TCTCCTTGAT
    GTTGTGCTAG ACAGTTTCAT CAAAACCAGT GCAACAGGTG GCTTGGGATC AATAAAAGCC
    GAGGTGATGG CAGATACTGC TGTAGCTCTG GCGTCTGGAA ATGTGAAATT GGTTTCTAGC
    AAGGTTATTG GAAGGATGTG CAAAATAATT GACAAGACAT GCTTATCTCC AACTCCTACT
    TTAGAACAGC ATCTTATGTG GGATGACATT GCGATTCTAG CGCGCTACAT GCTGATGTTG
    TCCTTCAACA ACTCCCTCGA CGTGGCGGCT CATCTCCCCT ACCTCTTCCA CGTTGTGACT
    TTCTTAGTAG CCACAGGGCC ACTCTCCCTT AGAGCTTCCA CACATGGATT GGTCATTAAT
    ATCATTCACT CTCTGTGCAC TTGTTCACAG CTTCATTTTA GTGAGGAGAC CAAGCAAGTT
    TTGAGACTCA GTTTGACAGA GTTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CAGCTGCTGT GATTGCCTTC CGCTCCAGTT ACCGGGACAG GTCGTTCTCT
    CCTGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCT TGGAAACAGT CACGGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG GTTTGCATTC CAGTACAACC CGTCCCTGCA ACCGAGAGCT
    CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA GTGTCTCACG GGCAGATAAA GCAGATTATC
    CGCATTCTTA GCAAGGCGCT TGAGAGTTGC TTAAAAGGAC CTGATACTTA CAACAGTCAA
    GTTCTGATAG AAGCTACAGT AATAGCACTA ACCAAATTAC AACCACTTCT TAATAAGGAC
    TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA GCTGTGGCCG TGCTGCAGCT CGATGAGGTC
    AACTTGTATT CAGCAGGCAC CGCACTTCTT GAGCAAAACC TGCACACCTT AGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCAGAGGAA GTATTTATGG CAATTCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAAATGGA TCATTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT
    GCACTGGTTG GACACCTCTT AAAAGGGTAC AGACATCCTT CACCTGCTAT TGTTGCACGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTGGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACGCAGAG CGTGGCTTAC TTAGCAGCTT TACTCACAGT ATCTGAGGAG
    GTTCGAAGTC GCTGTAGCCT AAAACATAGA AAGTCTCTTC TTCTTACTGA TATTTCAATG
    GAAAATATTC CTATGGATAC ATATCCCATT CATCATGGCG ACCCCAGCTA TAGGACACTA
    AAGGAGAGTC AGCCATGGTC CTCTCCCAAA GGCTCGGAAG GGTACCTTGC AGCCACCTAT
    CCAACCGTGG GCCAGACCAG CCCCCGAGCC CGGAAATCCA TGAGCTTGGA CATGGGGCAG
    CCTTCTCAGG CCAACACTAA GAAGTTGCTG GGAACAAGGA AAAGTTTTGA TCACTTGATA
    TCAGACACGA AGGCTCCTAA AAGGCAAGAA GTGGAATCAG GGATCACAAC ACCCCCCAAA
    ATGAGGAGAG TAGCAGAAAC TGATTATGAA ATGGAAACTC AAAGGATTTC CTCATCACAA
    CAGCACCCAC ATTTACGTAA AGTTTCAGTG TCTGAATCAA ATGTTCTCTT GGATGAAGAA
    GTACTTACTG ACCCGAAGAT CCAGGCATTG CTGCTTACTG TTCTGGCTAC ACTGGTGAAA
    TACACCACGG ATGAGTTTGA TCAACGAATT CTTTATGAAT ACTTAGCAGA AGCCAGTGTT
    GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC AATTTGTTGG ACTCCAAGAT CAACACCCTG
    TTGTCATTGT GCCAAGATCC AAATTTGTTA AATCCGATTC ATGGGATTGT GCAGAGTGTG
    GTATACTACG AGGAGTCCCC ACCACAGTAC CAGACGTCTT ACCTGCAGAG TTTTGGTTTT
    AATGGCTTGT GGCGGTTTGC AGGACCGTTT TCAAAGCAAA CACAAATCCC AGACTATGCT
    GAGCTTATTG TTAAGTTTCT TGATGCCTTG ATTGACACGT ATTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ATCCTCCTGC AGTGCAGAGC
    CAGCTTAGTA TTACTGCTAA CCTTAACCTT TCCAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGGAATCGA CAAGGAGAAC GTTGAACTCT CCCCAACCAC TGGGCACTGT
    AACAGTGGAC GAACTCGCCA TGGATCCGCA AGCCAAGTGC AGAAGCAAAG AAGCGCTGGC
    AGTTTCAAAC GTAATAGCAT TAAGAAGATC GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45485
    Clone ID Related Accession (Same CDS sequence) XM_023653057.1
    Accession Version XM_023653057.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGGAGTTCC TGTCACTTTC TCTTCCTTTA TGGGGTAGGA
    TGTGATGTTC CTTCCAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAGTTATTA
    CGTACTTATA CTCCTGGAGC CTCTCTCCGG AAGGGAAAAG GAAACTCATC CATGGATAGT
    GCGGCAGGGT GCAGTGGAAC GCCGCCGATA TGCCGGCAAG CCCAGACCAA ACTGGAAGTG
    GCCCTGTACA TGTTTCTGTG GAGCCCTGAC ACCGAAGCTG TTCTGGTTGC CATGTCCTGT
    TTCCGCCACC TCTGTGAGGA AGCGGATATC CGGTGTGGGG TGGACGAAGT ATCGGTGCAC
    AACTTCTTGC CGAACTATAA CACATTCATG GAATTTGCGT CTGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCACTTCA GAAAAGAGTG ATGGCACTAC TGAGGCGCAT TGAACATCCC
    ACTGCAGGAA ACACTGAGGC TTGGGAAGAT ACACATGCAA AGTGGGAACA AGCTACAAAA
    CTAATCCTCA ACTATCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCTGA AAGCCTTCAT
    AAGACCATTG TTAAGAGGCG AATGTCTCAT GTGAGTGGAG GAGGATCCAT AGATTTGTCT
    GACACGGACT CCCTTCAGGA ATGGATCAAC ATGACCGGCT TCCTCTGTGC CCTTGGGGGA
    GTGTGCCTGC AGCAGAGAAG CAATTCTGGC CTGGCAACCT ATAGCCCACC CATGGGCCCA
    GTCAGTGAAC GCAAGGGTTC CATGATTTCA GTGATGTCCT CAGAGGGAAA TGCAGACACA
    CCTGTCAGCA AATTTATGGA TCGGCTGCTA TCCTTAATGG TGTGTAACCA TGAGAAAGTG
    GGACTTCAAA TACGCACCAA CGTTAAGGAT CTGGTGGGTT TAGAATTGAG TCCTGCTCTT
    TATCCAATGC TGTTTAACAA ATTGAAGAAC ACCATCAGCA AGTTTTTTGA CTCCCAAGGA
    CAGGTTTTAT TGACTGACAC CAATACTCAA TTTGTAGAGC AAACCATAGC TATAATGAAG
    AACTTACTAG ATAATCATAC TGAAGGCAGC TCCGAACATC TGGGGCAAGC TAGCATTGAA
    ACGATGATGT TAAATCTGGT CAGATATGTT CGTGTACTTG GGAACATGGT CCATGCAATT
    CAAATAAAAA CGAAACTGTG TCAGCTAGTC GAGGTAATGA TGGCAAGAAG AGATGACCTC
    TCATTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTGAC AGATTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GATGTAAAAT GTCTTACAAG AGATCTGGAC
    CAGGCAAGCA TGGAAGCAGT AGTTTCACTC CTGGCTGGTC TCCCGCTGCA GCCTGAGGAA
    GGAGATGGTG TGGAACTCAT GGAAGCCAAG TCACAGCTAT TTCTTAAGTA TTTCACATTG
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACAGGTGGC
    AGGAAGCGTG GCATGTCTCG GAGGCTGGCC TCACTGAGGC ACTGCACAGT CCTTGCAATG
    TCAAACTTAC TTAACGCTAA CGTGGACAGT GGCCTCATGC ACTCCATAGG TTTAGGTTAT
    CACAAAGATC TCCAGACAAG AGCTACATTT ATGGAAGTTC TGACAAAAAT CCTCCAACAA
    GGCACAGAAT TTGACACACT TGCAGAAACG GTGTTGGCAG ATCGGTTTGA GAGGCTGGTG
    GAGTTGGTCA CAATGATGGG TGATCAGGGA GAGCTCCCTA TAGCCATGGC TCTGGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAACTA GCTCGAGTTC TAGTGACTCT CTTTGATTCT
    CGGCATTTAC TCTACCAACT GCTCTGGAAC ATGTTTTCTA AGGAAGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTTTTCCG AGGCAACAGC TTAGCCAGTA AAATAATGAC CTTCTGTTTC
    AAGGTATATG GTGCTACCTA CCTACAAAAA CTCCTGGATC CTTTGTTACG AATTGTGATC
    ACATCCTCTG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACAGTCA
    GAGAGCCTTG AGGAGAACCA GCGGAGCCTC CTTCAGATGA CTGAAAAGTT CTTCCACGCC
    ATCATCAGTT CCTCCTCGGA ATTCCCCCCT CAGCTTCGAA GTGTGTGCCA CTGTCTATAC
    CAGGCAACTT GCCACTCCCT ACTGAATACA GCTACAGTAA AAGAAAAAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG TTTCCCTCAG AACAGCATCG GTGCAGTGGG AAGTGCCATG
    TTCCTCAGAT TCATCAATCC TGCCATCGTC TCACCGTATG AAGCAGGCAT TTTAGATAAA
    AAGCCACCAC CTAGAATCGA AAGGGGCTTG AAGTTAATGT CAAAGATACT TCAGAGTATT
    GCCAATCATG TTCTCTTCAC AAAAGAAGAG CATATGCGGC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ATGCAGCACG AAGGTTTTTC CTTGATATAG CATCCGATTG TCCTACAAGT
    GATGCAGTGA ATCACAGTCT TTCCTTCATC AGCGATGGCA ACGTGCTGGC TCTACATCGT
    CTCCTCTGGA ACAATCAAGA GAAGATTGGC CAGTATCTTT CCAGCAATAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCAACACTTC TTGCATACCT GGGTCCTCCA
    GAGCACAAAC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTC
    GAGGAATTTA TGACTAGGCA TCAAGTACAT GAAAAAGAAG AGTTCAAGGC CTTGAAAACA
    TTAAGTATTT TCTACCAAGC TGGGACTTCC AGAGCTGGGA ATCCTATATT TTATTATGTT
    GCACGGAGGT TCAAAACTGG TCAAATCAAT GGCGATTTGC TGATCTACCA TGTCTTGCTG
    ACTCTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACCGGG
    CCTAGCAATC GCTTTAAAAC GGACTTTCTC TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT
    GCTTATGACA ATGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAC
    ACCAAGTATC ACGAGCGGCT GCTCACTGGC CTCAAAGGCA GCAAAAGGCT CATTTTCATA
    GACTGTCCTG GGAAGCTGGC TGAGCACGTA GAGCACGAAC AACAGAAACT ACCTGCTGCC
    ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG TTCCACAATG CTCTCAAGCT GGCTCACAAA
    GACACCAAGG TTTCCATTAA AGTTGGTTCT ACTGCTGTTC AAGTGACCTC AGCAGAGCGA
    ACGAAAGTCC TAGGGCAATC AGTCTTTCTA AACGATATCT ACTATGCTTC GGAAATTGAA
    GAGATCTGCC TGGTGGATGA GAACCAGTTC ACCTTAACCA TTGCAAACCA GGGCACACCG
    CTCACCTTCA TGCACCAGGA GTGTGAAGCC ATCGTCCAGT CCATCATTCA CATCCGGACA
    CGCTGGGAGC TGTCACAGCC CGACTCCATC CCCCAGCACA CCAAGATCCG GCCAAAAGAT
    GTCCCTGGGA CACTGCTCAA TATCGCGTTA CTTAATTTAG GCAGTTCAGA CCCTAGTTTA
    CGGTCAGCTG CCTATAATCT TCTGTGTGCC TTAACTTGTA CCTTTAATTT AAAAATCGAG
    GGCCAGTTGC TAGAGACGTC AGGTTTATGT ATCCCTGCCA ACAACACCCT CTTTATTGTC
    TCTATTAGTA AGACACTGGC AGCCAATGAG CCACACCTCA CCTTAGAATT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAACTGAAAC ACCTTTGTTT GGAATACATG
    ACTCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCACA ATGATGATGC CAAAAGACAA
    AGAGTTACTG CTATTCTTGA TAAGCTGATA ACAATGACCA TAAATGAGAA ACAGATGTAC
    CCATCTATTC AAGCAAAAAT ATGGGGGAGC CTTGGGCAGA TTACAGATCT CCTTGATGTT
    GTGCTAGACA GTTTCATCAA AACCAGTGCA ACAGGTGGCT TGGGATCAAT AAAAGCCGAG
    GTGATGGCAG ATACTGCTGT AGCTCTGGCG TCTGGAAATG TGAAATTGGT TTCTAGCAAG
    GTTATTGGAA GGATGTGCAA AATAATTGAC AAGACATGCT TATCTCCAAC TCCTACTTTA
    GAACAGCATC TTATGTGGGA TGACATTGCG ATTCTAGCGC GCTACATGCT GATGTTGTCC
    TTCAACAACT CCCTCGACGT GGCGGCTCAT CTCCCCTACC TCTTCCACGT TGTGACTTTC
    TTAGTAGCCA CAGGGCCACT CTCCCTTAGA GCTTCCACAC ATGGATTGGT CATTAATATC
    ATTCACTCTC TGTGCACTTG TTCACAGCTT CATTTTAGTG AGGAGACCAA GCAAGTTTTG
    AGACTCAGTT TGACAGAGTT CTCATTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC TCCAGTTACC GGGACAGGTC GTTCTCTCCT
    GGCTCCTACG AGAGGGAGAC TTTTGCTTTG ACATCCTTGG AAACAGTCAC GGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGGTT TGCATTCCAG TACAACCCGT CCCTGCAACC GAGAGCTCTG
    GTCGTCTTTG GCTGCATTAG TAAGCGAGTG TCTCACGGGC AGATAAAGCA GATTATCCGC
    ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA AAAGGACCTG ATACTTACAA CAGTCAAGTT
    CTGATAGAAG CTACAGTAAT AGCACTAACC AAATTACAAC CACTTCTTAA TAAGGACTCC
    CCTCTGCACA AAGCCCTCTT TTGGGTAGCT GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC
    TTGTATTCAG CAGGCACCGC ACTTCTTGAG CAAAACCTGC ACACCTTAGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC AGAGGAAGTA TTTATGGCAA TTCGGAATCC TCTGGAATGG
    CACTGCAAGC AAATGGATCA TTTTGTTGGA CTCAATTTCA ACTCTAACTT TAACTTTGCA
    CTGGTTGGAC ACCTCTTAAA AGGGTACAGA CATCCTTCAC CTGCTATTGT TGCACGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTG GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CGCAGAGCGT GGCTTACTTA GCAGCTTTAC TCACAGTATC TGAGGAGGTT
    CGAAGTCGCT GTAGCCTAAA ACATAGAAAG TCTCTTCTTC TTACTGATAT TTCAATGGAA
    AATATTCCTA TGGATACATA TCCCATTCAT CATGGCGACC CCAGCTATAG GACACTAAAG
    GAGAGTCAGC CATGGTCCTC TCCCAAAGGC TCGGAAGGGT ACCTTGCAGC CACCTATCCA
    ACCGTGGGCC AGACCAGCCC CCGAGCCCGG AAATCCATGA GCTTGGACAT GGGGCAGCCT
    TCTCAGGCCA ACACTAAGAA GTTGCTGGGA ACAAGGAAAA GTTTTGATCA CTTGATATCA
    GACACGAAGG CTCCTAAAAG GCAAGAAGTG GAATCAGGGA TCACAACACC CCCCAAAATG
    AGGAGAGTAG CAGAAACTGA TTATGAAATG GAAACTCAAA GGATTTCCTC ATCACAACAG
    CACCCACATT TACGTAAAGT TTCAGTGTCT GAATCAAATG TTCTCTTGGA TGAAGAAGTA
    CTTACTGACC CGAAGATCCA GGCATTGCTG CTTACTGTTC TGGCTACACT GGTGAAATAC
    ACCACGGATG AGTTTGATCA ACGAATTCTT TATGAATACT TAGCAGAAGC CAGTGTTGTT
    TTCCCCAAAG TTTTTCCTGT TGTGCACAAT TTGTTGGACT CCAAGATCAA CACCCTGTTG
    TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCGATTCATG GGATTGTGCA GAGTGTGGTA
    TACTACGAGG AGTCCCCACC ACAGTACCAG ACGTCTTACC TGCAGAGTTT TGGTTTTAAT
    GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG
    CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTATT TGCCTGGAAT TGATGAAGAA
    ACCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTATC CTCCTGCAGT GCAGAGCCAG
    CTTAGTATTA CTGCTAACCT TAACCTTTCC AATTCCATGA CCTCACTTGC AACTTCCCAG
    CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CAACCACTGG GCACTGTAAC
    AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT
    TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508825.1
    CDS165..8717
    Translation

    Target ORF information:

    RefSeq Version XM_023653057.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGGAGTTCC TGTCACTTTC TCTTCCTTTA TGGGGTAGGA
    TGTGATGTTC CTTCCAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAGTTATTA
    CGTACTTATA CTCCTGGAGC CTCTCTCCGG AAGGGAAAAG GAAACTCATC CATGGATAGT
    GCGGCAGGGT GCAGTGGAAC GCCGCCGATA TGCCGGCAAG CCCAGACCAA ACTGGAAGTG
    GCCCTGTACA TGTTTCTGTG GAGCCCTGAC ACCGAAGCTG TTCTGGTTGC CATGTCCTGT
    TTCCGCCACC TCTGTGAGGA AGCGGATATC CGGTGTGGGG TGGACGAAGT ATCGGTGCAC
    AACTTCTTGC CGAACTATAA CACATTCATG GAATTTGCGT CTGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCACTTCA GAAAAGAGTG ATGGCACTAC TGAGGCGCAT TGAACATCCC
    ACTGCAGGAA ACACTGAGGC TTGGGAAGAT ACACATGCAA AGTGGGAACA AGCTACAAAA
    CTAATCCTCA ACTATCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCTGA AAGCCTTCAT
    AAGACCATTG TTAAGAGGCG AATGTCTCAT GTGAGTGGAG GAGGATCCAT AGATTTGTCT
    GACACGGACT CCCTTCAGGA ATGGATCAAC ATGACCGGCT TCCTCTGTGC CCTTGGGGGA
    GTGTGCCTGC AGCAGAGAAG CAATTCTGGC CTGGCAACCT ATAGCCCACC CATGGGCCCA
    GTCAGTGAAC GCAAGGGTTC CATGATTTCA GTGATGTCCT CAGAGGGAAA TGCAGACACA
    CCTGTCAGCA AATTTATGGA TCGGCTGCTA TCCTTAATGG TGTGTAACCA TGAGAAAGTG
    GGACTTCAAA TACGCACCAA CGTTAAGGAT CTGGTGGGTT TAGAATTGAG TCCTGCTCTT
    TATCCAATGC TGTTTAACAA ATTGAAGAAC ACCATCAGCA AGTTTTTTGA CTCCCAAGGA
    CAGGTTTTAT TGACTGACAC CAATACTCAA TTTGTAGAGC AAACCATAGC TATAATGAAG
    AACTTACTAG ATAATCATAC TGAAGGCAGC TCCGAACATC TGGGGCAAGC TAGCATTGAA
    ACGATGATGT TAAATCTGGT CAGATATGTT CGTGTACTTG GGAACATGGT CCATGCAATT
    CAAATAAAAA CGAAACTGTG TCAGCTAGTC GAGGTAATGA TGGCAAGAAG AGATGACCTC
    TCATTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTGAC AGATTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GATGTAAAAT GTCTTACAAG AGATCTGGAC
    CAGGCAAGCA TGGAAGCAGT AGTTTCACTC CTGGCTGGTC TCCCGCTGCA GCCTGAGGAA
    GGAGATGGTG TGGAACTCAT GGAAGCCAAG TCACAGCTAT TTCTTAAGTA TTTCACATTG
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACAGGTGGC
    AGGAAGCGTG GCATGTCTCG GAGGCTGGCC TCACTGAGGC ACTGCACAGT CCTTGCAATG
    TCAAACTTAC TTAACGCTAA CGTGGACAGT GGCCTCATGC ACTCCATAGG TTTAGGTTAT
    CACAAAGATC TCCAGACAAG AGCTACATTT ATGGAAGTTC TGACAAAAAT CCTCCAACAA
    GGCACAGAAT TTGACACACT TGCAGAAACG GTGTTGGCAG ATCGGTTTGA GAGGCTGGTG
    GAGTTGGTCA CAATGATGGG TGATCAGGGA GAGCTCCCTA TAGCCATGGC TCTGGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAACTA GCTCGAGTTC TAGTGACTCT CTTTGATTCT
    CGGCATTTAC TCTACCAACT GCTCTGGAAC ATGTTTTCTA AGGAAGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTTTTCCG AGGCAACAGC TTAGCCAGTA AAATAATGAC CTTCTGTTTC
    AAGGTATATG GTGCTACCTA CCTACAAAAA CTCCTGGATC CTTTGTTACG AATTGTGATC
    ACATCCTCTG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACAGTCA
    GAGAGCCTTG AGGAGAACCA GCGGAGCCTC CTTCAGATGA CTGAAAAGTT CTTCCACGCC
    ATCATCAGTT CCTCCTCGGA ATTCCCCCCT CAGCTTCGAA GTGTGTGCCA CTGTCTATAC
    CAGGCAACTT GCCACTCCCT ACTGAATACA GCTACAGTAA AAGAAAAAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG TTTCCCTCAG AACAGCATCG GTGCAGTGGG AAGTGCCATG
    TTCCTCAGAT TCATCAATCC TGCCATCGTC TCACCGTATG AAGCAGGCAT TTTAGATAAA
    AAGCCACCAC CTAGAATCGA AAGGGGCTTG AAGTTAATGT CAAAGATACT TCAGAGTATT
    GCCAATCATG TTCTCTTCAC AAAAGAAGAG CATATGCGGC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ATGCAGCACG AAGGTTTTTC CTTGATATAG CATCCGATTG TCCTACAAGT
    GATGCAGTGA ATCACAGTCT TTCCTTCATC AGCGATGGCA ACGTGCTGGC TCTACATCGT
    CTCCTCTGGA ACAATCAAGA GAAGATTGGC CAGTATCTTT CCAGCAATAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCAACACTTC TTGCATACCT GGGTCCTCCA
    GAGCACAAAC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTC
    GAGGAATTTA TGACTAGGCA TCAAGTACAT GAAAAAGAAG AGTTCAAGGC CTTGAAAACA
    TTAAGTATTT TCTACCAAGC TGGGACTTCC AGAGCTGGGA ATCCTATATT TTATTATGTT
    GCACGGAGGT TCAAAACTGG TCAAATCAAT GGCGATTTGC TGATCTACCA TGTCTTGCTG
    ACTCTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACCGGG
    CCTAGCAATC GCTTTAAAAC GGACTTTCTC TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT
    GCTTATGACA ATGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAC
    ACCAAGTATC ACGAGCGGCT GCTCACTGGC CTCAAAGGCA GCAAAAGGCT CATTTTCATA
    GACTGTCCTG GGAAGCTGGC TGAGCACGTA GAGCACGAAC AACAGAAACT ACCTGCTGCC
    ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG TTCCACAATG CTCTCAAGCT GGCTCACAAA
    GACACCAAGG TTTCCATTAA AGTTGGTTCT ACTGCTGTTC AAGTGACCTC AGCAGAGCGA
    ACGAAAGTCC TAGGGCAATC AGTCTTTCTA AACGATATCT ACTATGCTTC GGAAATTGAA
    GAGATCTGCC TGGTGGATGA GAACCAGTTC ACCTTAACCA TTGCAAACCA GGGCACACCG
    CTCACCTTCA TGCACCAGGA GTGTGAAGCC ATCGTCCAGT CCATCATTCA CATCCGGACA
    CGCTGGGAGC TGTCACAGCC CGACTCCATC CCCCAGCACA CCAAGATCCG GCCAAAAGAT
    GTCCCTGGGA CACTGCTCAA TATCGCGTTA CTTAATTTAG GCAGTTCAGA CCCTAGTTTA
    CGGTCAGCTG CCTATAATCT TCTGTGTGCC TTAACTTGTA CCTTTAATTT AAAAATCGAG
    GGCCAGTTGC TAGAGACGTC AGGTTTATGT ATCCCTGCCA ACAACACCCT CTTTATTGTC
    TCTATTAGTA AGACACTGGC AGCCAATGAG CCACACCTCA CCTTAGAATT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAACTGAAAC ACCTTTGTTT GGAATACATG
    ACTCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCACA ATGATGATGC CAAAAGACAA
    AGAGTTACTG CTATTCTTGA TAAGCTGATA ACAATGACCA TAAATGAGAA ACAGATGTAC
    CCATCTATTC AAGCAAAAAT ATGGGGGAGC CTTGGGCAGA TTACAGATCT CCTTGATGTT
    GTGCTAGACA GTTTCATCAA AACCAGTGCA ACAGGTGGCT TGGGATCAAT AAAAGCCGAG
    GTGATGGCAG ATACTGCTGT AGCTCTGGCG TCTGGAAATG TGAAATTGGT TTCTAGCAAG
    GTTATTGGAA GGATGTGCAA AATAATTGAC AAGACATGCT TATCTCCAAC TCCTACTTTA
    GAACAGCATC TTATGTGGGA TGACATTGCG ATTCTAGCGC GCTACATGCT GATGTTGTCC
    TTCAACAACT CCCTCGACGT GGCGGCTCAT CTCCCCTACC TCTTCCACGT TGTGACTTTC
    TTAGTAGCCA CAGGGCCACT CTCCCTTAGA GCTTCCACAC ATGGATTGGT CATTAATATC
    ATTCACTCTC TGTGCACTTG TTCACAGCTT CATTTTAGTG AGGAGACCAA GCAAGTTTTG
    AGACTCAGTT TGACAGAGTT CTCATTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC TCCAGTTACC GGGACAGGTC GTTCTCTCCT
    GGCTCCTACG AGAGGGAGAC TTTTGCTTTG ACATCCTTGG AAACAGTCAC GGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGGTT TGCATTCCAG TACAACCCGT CCCTGCAACC GAGAGCTCTG
    GTCGTCTTTG GCTGCATTAG TAAGCGAGTG TCTCACGGGC AGATAAAGCA GATTATCCGC
    ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA AAAGGACCTG ATACTTACAA CAGTCAAGTT
    CTGATAGAAG CTACAGTAAT AGCACTAACC AAATTACAAC CACTTCTTAA TAAGGACTCC
    CCTCTGCACA AAGCCCTCTT TTGGGTAGCT GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC
    TTGTATTCAG CAGGCACCGC ACTTCTTGAG CAAAACCTGC ACACCTTAGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC AGAGGAAGTA TTTATGGCAA TTCGGAATCC TCTGGAATGG
    CACTGCAAGC AAATGGATCA TTTTGTTGGA CTCAATTTCA ACTCTAACTT TAACTTTGCA
    CTGGTTGGAC ACCTCTTAAA AGGGTACAGA CATCCTTCAC CTGCTATTGT TGCACGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTG GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CGCAGAGCGT GGCTTACTTA GCAGCTTTAC TCACAGTATC TGAGGAGGTT
    CGAAGTCGCT GTAGCCTAAA ACATAGAAAG TCTCTTCTTC TTACTGATAT TTCAATGGAA
    AATATTCCTA TGGATACATA TCCCATTCAT CATGGCGACC CCAGCTATAG GACACTAAAG
    GAGAGTCAGC CATGGTCCTC TCCCAAAGGC TCGGAAGGGT ACCTTGCAGC CACCTATCCA
    ACCGTGGGCC AGACCAGCCC CCGAGCCCGG AAATCCATGA GCTTGGACAT GGGGCAGCCT
    TCTCAGGCCA ACACTAAGAA GTTGCTGGGA ACAAGGAAAA GTTTTGATCA CTTGATATCA
    GACACGAAGG CTCCTAAAAG GCAAGAAGTG GAATCAGGGA TCACAACACC CCCCAAAATG
    AGGAGAGTAG CAGAAACTGA TTATGAAATG GAAACTCAAA GGATTTCCTC ATCACAACAG
    CACCCACATT TACGTAAAGT TTCAGTGTCT GAATCAAATG TTCTCTTGGA TGAAGAAGTA
    CTTACTGACC CGAAGATCCA GGCATTGCTG CTTACTGTTC TGGCTACACT GGTGAAATAC
    ACCACGGATG AGTTTGATCA ACGAATTCTT TATGAATACT TAGCAGAAGC CAGTGTTGTT
    TTCCCCAAAG TTTTTCCTGT TGTGCACAAT TTGTTGGACT CCAAGATCAA CACCCTGTTG
    TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCGATTCATG GGATTGTGCA GAGTGTGGTA
    TACTACGAGG AGTCCCCACC ACAGTACCAG ACGTCTTACC TGCAGAGTTT TGGTTTTAAT
    GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG
    CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTATT TGCCTGGAAT TGATGAAGAA
    ACCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTATC CTCCTGCAGT GCAGAGCCAG
    CTTAGTATTA CTGCTAACCT TAACCTTTCC AATTCCATGA CCTCACTTGC AACTTCCCAG
    CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CAACCACTGG GCACTGTAAC
    AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT
    TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45486
    Clone ID Related Accession (Same CDS sequence) XM_023653058.1
    Accession Version XM_023653058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGCAA CTTGCCACTC CCTACTGAAT ACAGCTACAG TAAAAGAAAA AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGTTTCCCT CAGAACAGCA TCGGTGCAGT GGGAAGTGCC
    ATGTTCCTCA GATTCATCAA TCCTGCCATC GTCTCACCGT ATGAAGCAGG CATTTTAGAT
    AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT
    ATTGCCAATC ATGTTCTCTT CACAAAAGAA GAGCATATGC GGCCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGATGCAGC ACGAAGGTTT TTCCTTGATA TAGCATCCGA TTGTCCTACA
    AGTGATGCAG TGAATCACAG TCTTTCCTTC ATCAGCGATG GCAACGTGCT GGCTCTACAT
    CGTCTCCTCT GGAACAATCA AGAGAAGATT GGCCAGTATC TTTCCAGCAA TAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCAACAC TTCTTGCATA CCTGGGTCCT
    CCAGAGCACA AACCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTCGAGGAAT TTATGACTAG GCATCAAGTA CATGAAAAAG AAGAGTTCAA GGCCTTGAAA
    ACATTAAGTA TTTTCTACCA AGCTGGGACT TCCAGAGCTG GGAATCCTAT ATTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGTCAAATC AATGGCGATT TGCTGATCTA CCATGTCTTG
    CTGACTCTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACC
    GGGCCTAGCA ATCGCTTTAA AACGGACTTT CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC
    TTTGCTTATG ACAATGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TACACCAAGT ATCACGAGCG GCTGCTCACT GGCCTCAAAG GCAGCAAAAG GCTCATTTTC
    ATAGACTGTC CTGGGAAGCT GGCTGAGCAC GTAGAGCACG AACAACAGAA ACTACCTGCT
    GCCACTTTGG CTTTAGAAGA GGACCTGAAA GTGTTCCACA ATGCTCTCAA GCTGGCTCAC
    AAAGACACCA AGGTTTCCAT TAAAGTTGGT TCTACTGCTG TTCAAGTGAC CTCAGCAGAG
    CGAACGAAAG TCCTAGGGCA ATCAGTCTTT CTAAACGATA TCTACTATGC TTCGGAAATT
    GAAGAGATCT GCCTGGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAGGGCACA
    CCGCTCACCT TCATGCACCA GGAGTGTGAA GCCATCGTCC AGTCCATCAT TCACATCCGG
    ACACGCTGGG AGCTGTCACA GCCCGACTCC ATCCCCCAGC ACACCAAGAT CCGGCCAAAA
    GATGTCCCTG GGACACTGCT CAATATCGCG TTACTTAATT TAGGCAGTTC AGACCCTAGT
    TTACGGTCAG CTGCCTATAA TCTTCTGTGT GCCTTAACTT GTACCTTTAA TTTAAAAATC
    GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA TGTATCCCTG CCAACAACAC CCTCTTTATT
    GTCTCTATTA GTAAGACACT GGCAGCCAAT GAGCCACACC TCACCTTAGA ATTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAACTGA AACACCTTTG TTTGGAATAC
    ATGACTCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ACAATGATGA TGCCAAAAGA
    CAAAGAGTTA CTGCTATTCT TGATAAGCTG ATAACAATGA CCATAAATGA GAAACAGATG
    TACCCATCTA TTCAAGCAAA AATATGGGGG AGCCTTGGGC AGATTACAGA TCTCCTTGAT
    GTTGTGCTAG ACAGTTTCAT CAAAACCAGT GCAACAGGTG GCTTGGGATC AATAAAAGCC
    GAGGTGATGG CAGATACTGC TGTAGCTCTG GCGTCTGGAA ATGTGAAATT GGTTTCTAGC
    AAGGTTATTG GAAGGATGTG CAAAATAATT GACAAGACAT GCTTATCTCC AACTCCTACT
    TTAGAACAGC ATCTTATGTG GGATGACATT GCGATTCTAG CGCGCTACAT GCTGATGTTG
    TCCTTCAACA ACTCCCTCGA CGTGGCGGCT CATCTCCCCT ACCTCTTCCA CGTTGTGACT
    TTCTTAGTAG CCACAGGGCC ACTCTCCCTT AGAGCTTCCA CACATGGATT GGTCATTAAT
    ATCATTCACT CTCTGTGCAC TTGTTCACAG CTTCATTTTA GTGAGGAGAC CAAGCAAGTT
    TTGAGACTCA GTTTGACAGA GTTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CAGCTGCTGT GATTGCCTTC CGCTCCAGTT ACCGGGACAG GTCGTTCTCT
    CCTGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCT TGGAAACAGT CACGGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG GTTTGCATTC CAGTACAACC CGTCCCTGCA ACCGAGAGCT
    CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA GTGTCTCACG GGCAGATAAA GCAGATTATC
    CGCATTCTTA GCAAGGCGCT TGAGAGTTGC TTAAAAGGAC CTGATACTTA CAACAGTCAA
    GTTCTGATAG AAGCTACAGT AATAGCACTA ACCAAATTAC AACCACTTCT TAATAAGGAC
    TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA GCTGTGGCCG TGCTGCAGCT CGATGAGGTC
    AACTTGTATT CAGCAGGCAC CGCACTTCTT GAGCAAAACC TGCACACCTT AGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCAGAGGAA GTATTTATGG CAATTCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAAATGGA TCATTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT
    GCACTGGTTG GACACCTCTT AAAAGGGTAC AGACATCCTT CACCTGCTAT TGTTGCACGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTGGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACGCAGAG CGTGGCTTAC TTAGCAGCTT TACTCACAGT ATCTGAGGAG
    GTTCGAAGTC GCTGTAGCCT AAAACATAGA AAGTCTCTTC TTCTTACTGA TATTTCAATG
    GAAAATATTC CTATGGATAC ATATCCCATT CATCATGGCG ACCCCAGCTA TAGGACACTA
    AAGGAGAGTC AGCCATGGTC CTCTCCCAAA GGCTCGGAAG GGTACCTTGC AGCCACCTAT
    CCAACCGTGG GCCAGACCAG CCCCCGAGCC CGGAAATCCA TGAGCTTGGA CATGGGGCAG
    CCTTCTCAGG CCAACACTAA GAAGTTGCTG GGAACAAGGA AAAGTTTTGA TCACTTGATA
    TCAGACACGA AGGCTCCTAA AAGGCAAGAA GTGGAATCAG GGATCACAAC ACCCCCCAAA
    ATGAGGAGAG TAGCAGAAAC TGATTATGAA ATGGAAACTC AAAGGATTTC CTCATCACAA
    CAGCACCCAC ATTTACGTAA AGTTTCAGTG TCTGAATCAA ATGTTCTCTT GGATGAAGAA
    GTACTTACTG ACCCGAAGAT CCAGGCATTG CTGCTTACTG TTCTGGCTAC ACTGGTGAAA
    TACACCACGG ATGAGTTTGA TCAACGAATT CTTTATGAAT ACTTAGCAGA AGCCAGTGTT
    GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC AATTTGTTGG ACTCCAAGAT CAACACCCTG
    TTGTCATTGT GCCAAGATCC AAATTTGTTA AATCCGATTC ATGGGATTGT GCAGAGTGTG
    GTATACTACG AGGAGTCCCC ACCACAGTAC CAGACGTCTT ACCTGCAGAG TTTTGGTTTT
    AATGGCTTGT GGCGGTTTGC AGGACCGTTT TCAAAGCAAA CACAAATCCC AGACTATGCT
    GAGCTTATTG TTAAGTTTCT TGATGCCTTG ATTGACACGT ATTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ATCCTCCTGC AGTGCAGAGC
    CAGCTTAGTA TTACTGCTAA CCTTAACCTT TCCAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGCTTCTCT GCCTTGCTCT AAATCAGCAG TTTCCATGCA GCTGTTCCCT
    CATCAAGGTA CTCTTTGCTT TCGTCACCTT TGCACGAAGT ACTGCTTCCC CTGCGCTCGT
    CCTGGGCACA TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508826.1
    CDS167..8704
    Translation

    Target ORF information:

    RefSeq Version XM_023653058.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGCAA CTTGCCACTC CCTACTGAAT ACAGCTACAG TAAAAGAAAA AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGTTTCCCT CAGAACAGCA TCGGTGCAGT GGGAAGTGCC
    ATGTTCCTCA GATTCATCAA TCCTGCCATC GTCTCACCGT ATGAAGCAGG CATTTTAGAT
    AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT
    ATTGCCAATC ATGTTCTCTT CACAAAAGAA GAGCATATGC GGCCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGATGCAGC ACGAAGGTTT TTCCTTGATA TAGCATCCGA TTGTCCTACA
    AGTGATGCAG TGAATCACAG TCTTTCCTTC ATCAGCGATG GCAACGTGCT GGCTCTACAT
    CGTCTCCTCT GGAACAATCA AGAGAAGATT GGCCAGTATC TTTCCAGCAA TAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCAACAC TTCTTGCATA CCTGGGTCCT
    CCAGAGCACA AACCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTCGAGGAAT TTATGACTAG GCATCAAGTA CATGAAAAAG AAGAGTTCAA GGCCTTGAAA
    ACATTAAGTA TTTTCTACCA AGCTGGGACT TCCAGAGCTG GGAATCCTAT ATTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGTCAAATC AATGGCGATT TGCTGATCTA CCATGTCTTG
    CTGACTCTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACC
    GGGCCTAGCA ATCGCTTTAA AACGGACTTT CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC
    TTTGCTTATG ACAATGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TACACCAAGT ATCACGAGCG GCTGCTCACT GGCCTCAAAG GCAGCAAAAG GCTCATTTTC
    ATAGACTGTC CTGGGAAGCT GGCTGAGCAC GTAGAGCACG AACAACAGAA ACTACCTGCT
    GCCACTTTGG CTTTAGAAGA GGACCTGAAA GTGTTCCACA ATGCTCTCAA GCTGGCTCAC
    AAAGACACCA AGGTTTCCAT TAAAGTTGGT TCTACTGCTG TTCAAGTGAC CTCAGCAGAG
    CGAACGAAAG TCCTAGGGCA ATCAGTCTTT CTAAACGATA TCTACTATGC TTCGGAAATT
    GAAGAGATCT GCCTGGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAGGGCACA
    CCGCTCACCT TCATGCACCA GGAGTGTGAA GCCATCGTCC AGTCCATCAT TCACATCCGG
    ACACGCTGGG AGCTGTCACA GCCCGACTCC ATCCCCCAGC ACACCAAGAT CCGGCCAAAA
    GATGTCCCTG GGACACTGCT CAATATCGCG TTACTTAATT TAGGCAGTTC AGACCCTAGT
    TTACGGTCAG CTGCCTATAA TCTTCTGTGT GCCTTAACTT GTACCTTTAA TTTAAAAATC
    GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA TGTATCCCTG CCAACAACAC CCTCTTTATT
    GTCTCTATTA GTAAGACACT GGCAGCCAAT GAGCCACACC TCACCTTAGA ATTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAACTGA AACACCTTTG TTTGGAATAC
    ATGACTCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ACAATGATGA TGCCAAAAGA
    CAAAGAGTTA CTGCTATTCT TGATAAGCTG ATAACAATGA CCATAAATGA GAAACAGATG
    TACCCATCTA TTCAAGCAAA AATATGGGGG AGCCTTGGGC AGATTACAGA TCTCCTTGAT
    GTTGTGCTAG ACAGTTTCAT CAAAACCAGT GCAACAGGTG GCTTGGGATC AATAAAAGCC
    GAGGTGATGG CAGATACTGC TGTAGCTCTG GCGTCTGGAA ATGTGAAATT GGTTTCTAGC
    AAGGTTATTG GAAGGATGTG CAAAATAATT GACAAGACAT GCTTATCTCC AACTCCTACT
    TTAGAACAGC ATCTTATGTG GGATGACATT GCGATTCTAG CGCGCTACAT GCTGATGTTG
    TCCTTCAACA ACTCCCTCGA CGTGGCGGCT CATCTCCCCT ACCTCTTCCA CGTTGTGACT
    TTCTTAGTAG CCACAGGGCC ACTCTCCCTT AGAGCTTCCA CACATGGATT GGTCATTAAT
    ATCATTCACT CTCTGTGCAC TTGTTCACAG CTTCATTTTA GTGAGGAGAC CAAGCAAGTT
    TTGAGACTCA GTTTGACAGA GTTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CAGCTGCTGT GATTGCCTTC CGCTCCAGTT ACCGGGACAG GTCGTTCTCT
    CCTGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCT TGGAAACAGT CACGGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG GTTTGCATTC CAGTACAACC CGTCCCTGCA ACCGAGAGCT
    CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA GTGTCTCACG GGCAGATAAA GCAGATTATC
    CGCATTCTTA GCAAGGCGCT TGAGAGTTGC TTAAAAGGAC CTGATACTTA CAACAGTCAA
    GTTCTGATAG AAGCTACAGT AATAGCACTA ACCAAATTAC AACCACTTCT TAATAAGGAC
    TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA GCTGTGGCCG TGCTGCAGCT CGATGAGGTC
    AACTTGTATT CAGCAGGCAC CGCACTTCTT GAGCAAAACC TGCACACCTT AGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCAGAGGAA GTATTTATGG CAATTCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAAATGGA TCATTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT
    GCACTGGTTG GACACCTCTT AAAAGGGTAC AGACATCCTT CACCTGCTAT TGTTGCACGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTGGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACGCAGAG CGTGGCTTAC TTAGCAGCTT TACTCACAGT ATCTGAGGAG
    GTTCGAAGTC GCTGTAGCCT AAAACATAGA AAGTCTCTTC TTCTTACTGA TATTTCAATG
    GAAAATATTC CTATGGATAC ATATCCCATT CATCATGGCG ACCCCAGCTA TAGGACACTA
    AAGGAGAGTC AGCCATGGTC CTCTCCCAAA GGCTCGGAAG GGTACCTTGC AGCCACCTAT
    CCAACCGTGG GCCAGACCAG CCCCCGAGCC CGGAAATCCA TGAGCTTGGA CATGGGGCAG
    CCTTCTCAGG CCAACACTAA GAAGTTGCTG GGAACAAGGA AAAGTTTTGA TCACTTGATA
    TCAGACACGA AGGCTCCTAA AAGGCAAGAA GTGGAATCAG GGATCACAAC ACCCCCCAAA
    ATGAGGAGAG TAGCAGAAAC TGATTATGAA ATGGAAACTC AAAGGATTTC CTCATCACAA
    CAGCACCCAC ATTTACGTAA AGTTTCAGTG TCTGAATCAA ATGTTCTCTT GGATGAAGAA
    GTACTTACTG ACCCGAAGAT CCAGGCATTG CTGCTTACTG TTCTGGCTAC ACTGGTGAAA
    TACACCACGG ATGAGTTTGA TCAACGAATT CTTTATGAAT ACTTAGCAGA AGCCAGTGTT
    GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC AATTTGTTGG ACTCCAAGAT CAACACCCTG
    TTGTCATTGT GCCAAGATCC AAATTTGTTA AATCCGATTC ATGGGATTGT GCAGAGTGTG
    GTATACTACG AGGAGTCCCC ACCACAGTAC CAGACGTCTT ACCTGCAGAG TTTTGGTTTT
    AATGGCTTGT GGCGGTTTGC AGGACCGTTT TCAAAGCAAA CACAAATCCC AGACTATGCT
    GAGCTTATTG TTAAGTTTCT TGATGCCTTG ATTGACACGT ATTTGCCTGG AATTGATGAA
    GAAACCAGTG AGGAGTCCCT CCTGACACCC ACATCTCCTT ATCCTCCTGC AGTGCAGAGC
    CAGCTTAGTA TTACTGCTAA CCTTAACCTT TCCAATTCCA TGACCTCACT TGCAACTTCC
    CAGCATTCCC CAGCTTCTCT GCCTTGCTCT AAATCAGCAG TTTCCATGCA GCTGTTCCCT
    CATCAAGGTA CTCTTTGCTT TCGTCACCTT TGCACGAAGT ACTGCTTCCC CTGCGCTCGT
    CCTGGGCACA TGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45487
    Clone ID Related Accession (Same CDS sequence) XM_023653059.1
    Accession Version XM_023653059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCACTGG ATTGGTGGCC TAAGATCGAT GCCGTATATT GTCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCG GTGCAGGGTT GTGGAGCACA CCCGGCGATA
    CGCATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTTT TGTCTATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCCAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTGCTTCATC AGTTAGACAG CATCGATTTG
    TGGAATCCTG ATGCTCCTGT GGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AGTTAACTAG CCAGCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGGAGT
    TCCTGTCACT TTCTCTTCCT TTATGGGGTA GGATGTGATG TTCCTTCCAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAGTTA TTACGTACTT ATACTCCTGG AGCCTCTCTC
    CGGAAGGGAA AAGGAAACTC ATCCATGGAT AGTGCGGCAG GGTGCAGTGG AACGCCGCCG
    ATATGCCGGC AAGCCCAGAC CAAACTGGAA GTGGCCCTGT ACATGTTTCT GTGGAGCCCT
    GACACCGAAG CTGTTCTGGT TGCCATGTCC TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT
    ATCCGGTGTG GGGTGGACGA AGTATCGGTG CACAACTTCT TGCCGAACTA TAACACATTC
    ATGGAATTTG CGTCTGTCAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCAC TACTGAGGCG CATTGAACAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACACATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CATAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTG TCTGACACGG ACTCCCTTCA GGAATGGATC
    AACATGACCG GCTTCCTCTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCCTGGCAA CCTATAGCCC ACCCATGGGC CCAGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CCTCAGAGGG AAATGCAGAC ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTATCCTTAA TGGTGTGTAA CCATGAGAAA GTGGGACTTC AAATACGCAC CAACGTTAAG
    GATCTGGTGG GTTTAGAATT GAGTCCTGCT CTTTATCCAA TGCTGTTTAA CAAATTGAAG
    AACACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAATTTGTAG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACTGAAGGC
    AGCTCCGAAC ATCTGGGGCA AGCTAGCATT GAAACGATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGGAACAT GGTCCATGCA ATTCAAATAA AAACGAAACT GTGTCAGCTA
    GTCGAGGTAA TGATGGCAAG AAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT GACAGATTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGATGTAA AATGTCTTAC AAGAGATCTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAACT CATGGAAGCC
    AAGTCACAGC TATTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAGC GTGGCATGTC TCGGAGGCTG
    GCCTCACTGA GGCACTGCAC AGTCCTTGCA ATGTCAAACT TACTTAACGC TAACGTGGAC
    AGTGGCCTCA TGCACTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACA
    TTTATGGAAG TTCTGACAAA AATCCTCCAA CAAGGCACAG AATTTGACAC ACTTGCAGAA
    ACGGTGTTGG CAGATCGGTT TGAGAGGCTG GTGGAGTTGG TCACAATGAT GGGTGATCAG
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAA
    CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT TCTCGGCATT TACTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCCATGC AGACTCTTTT CCGAGGCAAC
    AGCTTAGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAA
    AAACTCCTGG ATCCTTTGTT ACGAATTGTG ATCACATCCT CTGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC
    CTCCTTCAGA TGACTGAAAA GTTCTTCCAC GCCATCATCA GTTCCTCCTC GGAATTCCCC
    CCTCAGCTTC GAAGTGTGTG CCACTGTCTA TACCAGGCAA CTTGCCACTC CCTACTGAAT
    ACAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGTTTCCCT
    CAGAACAGCA TCGGTGCAGT GGGAAGTGCC ATGTTCCTCA GATTCATCAA TCCTGCCATC
    GTCTCACCGT ATGAAGCAGG CATTTTAGAT AAAAAGCCAC CACCTAGAAT CGAAAGGGGC
    TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT ATTGCCAATC ATGTTCTCTT CACAAAAGAA
    GAGCATATGC GGCCTTTTAA TGATTTTGTG AAAAGCAACT TTGATGCAGC ACGAAGGTTT
    TTCCTTGATA TAGCATCCGA TTGTCCTACA AGTGATGCAG TGAATCACAG TCTTTCCTTC
    ATCAGCGATG GCAACGTGCT GGCTCTACAT CGTCTCCTCT GGAACAATCA AGAGAAGATT
    GGCCAGTATC TTTCCAGCAA TAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCAACAC TTCTTGCATA CCTGGGTCCT CCAGAGCACA AACCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTCGAGGAAT TTATGACTAG GCATCAAGTA
    CATGAAAAAG AAGAGTTCAA GGCCTTGAAA ACATTAAGTA TTTTCTACCA AGCTGGGACT
    TCCAGAGCTG GGAATCCTAT ATTTTATTAT GTTGCACGGA GGTTCAAAAC TGGTCAAATC
    AATGGCGATT TGCTGATCTA CCATGTCTTG CTGACTCTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACC GGGCCTAGCA ATCGCTTTAA AACGGACTTT
    CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC TTTGCTTATG ACAATGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TACACCAAGT ATCACGAGCG GCTGCTCACT
    GGCCTCAAAG GCAGCAAAAG GCTCATTTTC ATAGACTGTC CTGGGAAGCT GGCTGAGCAC
    GTAGAGCACG AACAACAGAA ACTACCTGCT GCCACTTTGG CTTTAGAAGA GGACCTGAAA
    GTGTTCCACA ATGCTCTCAA GCTGGCTCAC AAAGACACCA AGGTTTCCAT TAAAGTTGGT
    TCTACTGCTG TTCAAGTGAC CTCAGCAGAG CGAACGAAAG TCCTAGGGCA ATCAGTCTTT
    CTAAACGATA TCTACTATGC TTCGGAAATT GAAGAGATCT GCCTGGTGGA TGAGAACCAG
    TTCACCTTAA CCATTGCAAA CCAGGGCACA CCGCTCACCT TCATGCACCA GGAGTGTGAA
    GCCATCGTCC AGTCCATCAT TCACATCCGG ACACGCTGGG AGCTGTCACA GCCCGACTCC
    ATCCCCCAGC ACACCAAGAT CCGGCCAAAA GATGTCCCTG GGACACTGCT CAATATCGCG
    TTACTTAATT TAGGCAGTTC AGACCCTAGT TTACGGTCAG CTGCCTATAA TCTTCTGTGT
    GCCTTAACTT GTACCTTTAA TTTAAAAATC GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA
    TGTATCCCTG CCAACAACAC CCTCTTTATT GTCTCTATTA GTAAGACACT GGCAGCCAAT
    GAGCCACACC TCACCTTAGA ATTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAACTGA AACACCTTTG TTTGGAATAC ATGACTCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ACAATGATGA TGCCAAAAGA CAAAGAGTTA CTGCTATTCT TGATAAGCTG
    ATAACAATGA CCATAAATGA GAAACAGATG TACCCATCTA TTCAAGCAAA AATATGGGGG
    AGCCTTGGGC AGATTACAGA TCTCCTTGAT GTTGTGCTAG ACAGTTTCAT CAAAACCAGT
    GCAACAGGTG GCTTGGGATC AATAAAAGCC GAGGTGATGG CAGATACTGC TGTAGCTCTG
    GCGTCTGGAA ATGTGAAATT GGTTTCTAGC AAGGTTATTG GAAGGATGTG CAAAATAATT
    GACAAGACAT GCTTATCTCC AACTCCTACT TTAGAACAGC ATCTTATGTG GGATGACATT
    GCGATTCTAG CGCGCTACAT GCTGATGTTG TCCTTCAACA ACTCCCTCGA CGTGGCGGCT
    CATCTCCCCT ACCTCTTCCA CGTTGTGACT TTCTTAGTAG CCACAGGGCC ACTCTCCCTT
    AGAGCTTCCA CACATGGATT GGTCATTAAT ATCATTCACT CTCTGTGCAC TTGTTCACAG
    CTTCATTTTA GTGAGGAGAC CAAGCAAGTT TTGAGACTCA GTTTGACAGA GTTCTCATTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CAGCTGCTGT GATTGCCTTC
    CGCTCCAGTT ACCGGGACAG GTCGTTCTCT CCTGGCTCCT ACGAGAGGGA GACTTTTGCT
    TTGACATCCT TGGAAACAGT CACGGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG GTTTGCATTC
    CAGTACAACC CGTCCCTGCA ACCGAGAGCT CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA
    GTGTCTCACG GGCAGATAAA GCAGATTATC CGCATTCTTA GCAAGGCGCT TGAGAGTTGC
    TTAAAAGGAC CTGATACTTA CAACAGTCAA GTTCTGATAG AAGCTACAGT AATAGCACTA
    ACCAAATTAC AACCACTTCT TAATAAGGAC TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA
    GCTGTGGCCG TGCTGCAGCT CGATGAGGTC AACTTGTATT CAGCAGGCAC CGCACTTCTT
    GAGCAAAACC TGCACACCTT AGACAGTCTC CGGATATTCA ATGACAAGAG TCCAGAGGAA
    GTATTTATGG CAATTCGGAA TCCTCTGGAA TGGCACTGCA AGCAAATGGA TCATTTTGTT
    GGACTCAATT TCAACTCTAA CTTTAACTTT GCACTGGTTG GACACCTCTT AAAAGGGTAC
    AGACATCCTT CACCTGCTAT TGTTGCACGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTGGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACGCAGAG CGTGGCTTAC
    TTAGCAGCTT TACTCACAGT ATCTGAGGAG GTTCGAAGTC GCTGTAGCCT AAAACATAGA
    AAGTCTCTTC TTCTTACTGA TATTTCAATG GAAAATATTC CTATGGATAC ATATCCCATT
    CATCATGGCG ACCCCAGCTA TAGGACACTA AAGGAGAGTC AGCCATGGTC CTCTCCCAAA
    GGCTCGGAAG GGTACCTTGC AGCCACCTAT CCAACCGTGG GCCAGACCAG CCCCCGAGCC
    CGGAAATCCA TGAGCTTGGA CATGGGGCAG CCTTCTCAGG CCAACACTAA GAAGTTGCTG
    GGAACAAGGA AAAGTTTTGA TCACTTGATA TCAGACACGA AGGCTCCTAA AAGGCAAGAA
    GTGGAATCAG GGATCACAAC ACCCCCCAAA ATGAGGAGAG TAGCAGAAAC TGATTATGAA
    ATGGAAACTC AAAGGATTTC CTCATCACAA CAGCACCCAC ATTTACGTAA AGTTTCAGTG
    TCTGAATCAA ATGTTCTCTT GGATGAAGAA GTACTTACTG ACCCGAAGAT CCAGGCATTG
    CTGCTTACTG TTCTGGCTAC ACTGGTGAAA TACACCACGG ATGAGTTTGA TCAACGAATT
    CTTTATGAAT ACTTAGCAGA AGCCAGTGTT GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCCAAGAT CAACACCCTG TTGTCATTGT GCCAAGATCC AAATTTGTTA
    AATCCGATTC ATGGGATTGT GCAGAGTGTG GTATACTACG AGGAGTCCCC ACCACAGTAC
    CAGACGTCTT ACCTGCAGAG TTTTGGTTTT AATGGCTTGT GGCGGTTTGC AGGACCGTTT
    TCAAAGCAAA CACAAATCCC AGACTATGCT GAGCTTATTG TTAAGTTTCT TGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ATCCTCCTGC AGTGCAGAGC CAGCTTAGTA TTACTGCTAA CCTTAACCTT
    TCCAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGAATCGA CAAGGAGAAC
    GTTGAACTCT CCCCAACCAC TGGGCACTGT AACAGTGGAC GAACTCGCCA TGGATCCGCA
    AGCCAAGTGC AGAAGCAAAG AAGCGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508827.1
    CDS165..8690
    Translation

    Target ORF information:

    RefSeq Version XM_023653059.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCACTGG ATTGGTGGCC TAAGATCGAT GCCGTATATT GTCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCG GTGCAGGGTT GTGGAGCACA CCCGGCGATA
    CGCATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTTT TGTCTATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCCAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTGCTTCATC AGTTAGACAG CATCGATTTG
    TGGAATCCTG ATGCTCCTGT GGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AGTTAACTAG CCAGCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGGAGT
    TCCTGTCACT TTCTCTTCCT TTATGGGGTA GGATGTGATG TTCCTTCCAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAGTTA TTACGTACTT ATACTCCTGG AGCCTCTCTC
    CGGAAGGGAA AAGGAAACTC ATCCATGGAT AGTGCGGCAG GGTGCAGTGG AACGCCGCCG
    ATATGCCGGC AAGCCCAGAC CAAACTGGAA GTGGCCCTGT ACATGTTTCT GTGGAGCCCT
    GACACCGAAG CTGTTCTGGT TGCCATGTCC TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT
    ATCCGGTGTG GGGTGGACGA AGTATCGGTG CACAACTTCT TGCCGAACTA TAACACATTC
    ATGGAATTTG CGTCTGTCAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCAC TACTGAGGCG CATTGAACAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACACATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CATAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTG TCTGACACGG ACTCCCTTCA GGAATGGATC
    AACATGACCG GCTTCCTCTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCCTGGCAA CCTATAGCCC ACCCATGGGC CCAGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CCTCAGAGGG AAATGCAGAC ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTATCCTTAA TGGTGTGTAA CCATGAGAAA GTGGGACTTC AAATACGCAC CAACGTTAAG
    GATCTGGTGG GTTTAGAATT GAGTCCTGCT CTTTATCCAA TGCTGTTTAA CAAATTGAAG
    AACACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAATTTGTAG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACTGAAGGC
    AGCTCCGAAC ATCTGGGGCA AGCTAGCATT GAAACGATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGGAACAT GGTCCATGCA ATTCAAATAA AAACGAAACT GTGTCAGCTA
    GTCGAGGTAA TGATGGCAAG AAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT GACAGATTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGATGTAA AATGTCTTAC AAGAGATCTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAACT CATGGAAGCC
    AAGTCACAGC TATTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAGC GTGGCATGTC TCGGAGGCTG
    GCCTCACTGA GGCACTGCAC AGTCCTTGCA ATGTCAAACT TACTTAACGC TAACGTGGAC
    AGTGGCCTCA TGCACTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACA
    TTTATGGAAG TTCTGACAAA AATCCTCCAA CAAGGCACAG AATTTGACAC ACTTGCAGAA
    ACGGTGTTGG CAGATCGGTT TGAGAGGCTG GTGGAGTTGG TCACAATGAT GGGTGATCAG
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAA
    CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT TCTCGGCATT TACTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCCATGC AGACTCTTTT CCGAGGCAAC
    AGCTTAGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAA
    AAACTCCTGG ATCCTTTGTT ACGAATTGTG ATCACATCCT CTGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC
    CTCCTTCAGA TGACTGAAAA GTTCTTCCAC GCCATCATCA GTTCCTCCTC GGAATTCCCC
    CCTCAGCTTC GAAGTGTGTG CCACTGTCTA TACCAGGCAA CTTGCCACTC CCTACTGAAT
    ACAGCTACAG TAAAAGAAAA AAAGGAAAAC AAAAAATCAG TGGTTAGCCA GCGTTTCCCT
    CAGAACAGCA TCGGTGCAGT GGGAAGTGCC ATGTTCCTCA GATTCATCAA TCCTGCCATC
    GTCTCACCGT ATGAAGCAGG CATTTTAGAT AAAAAGCCAC CACCTAGAAT CGAAAGGGGC
    TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT ATTGCCAATC ATGTTCTCTT CACAAAAGAA
    GAGCATATGC GGCCTTTTAA TGATTTTGTG AAAAGCAACT TTGATGCAGC ACGAAGGTTT
    TTCCTTGATA TAGCATCCGA TTGTCCTACA AGTGATGCAG TGAATCACAG TCTTTCCTTC
    ATCAGCGATG GCAACGTGCT GGCTCTACAT CGTCTCCTCT GGAACAATCA AGAGAAGATT
    GGCCAGTATC TTTCCAGCAA TAGGGATCAT AAAGCTGTTG GAAGACGACC TTTTGATAAG
    ATGGCAACAC TTCTTGCATA CCTGGGTCCT CCAGAGCACA AACCTGTGGC AGATACACAC
    TGGTCCAGCC TTAACCTTAC CAGTTCAAAG TTCGAGGAAT TTATGACTAG GCATCAAGTA
    CATGAAAAAG AAGAGTTCAA GGCCTTGAAA ACATTAAGTA TTTTCTACCA AGCTGGGACT
    TCCAGAGCTG GGAATCCTAT ATTTTATTAT GTTGCACGGA GGTTCAAAAC TGGTCAAATC
    AATGGCGATT TGCTGATCTA CCATGTCTTG CTGACTCTAA AGCCATATTA TGCAAAGCCA
    TATGAAATTG TAGTGGACCT TACCCATACC GGGCCTAGCA ATCGCTTTAA AACGGACTTT
    CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC TTTGCTTATG ACAATGTCTC TGCAGTCTAT
    ATCTATAACT GTAACTCCTG GGTCAGGGAG TACACCAAGT ATCACGAGCG GCTGCTCACT
    GGCCTCAAAG GCAGCAAAAG GCTCATTTTC ATAGACTGTC CTGGGAAGCT GGCTGAGCAC
    GTAGAGCACG AACAACAGAA ACTACCTGCT GCCACTTTGG CTTTAGAAGA GGACCTGAAA
    GTGTTCCACA ATGCTCTCAA GCTGGCTCAC AAAGACACCA AGGTTTCCAT TAAAGTTGGT
    TCTACTGCTG TTCAAGTGAC CTCAGCAGAG CGAACGAAAG TCCTAGGGCA ATCAGTCTTT
    CTAAACGATA TCTACTATGC TTCGGAAATT GAAGAGATCT GCCTGGTGGA TGAGAACCAG
    TTCACCTTAA CCATTGCAAA CCAGGGCACA CCGCTCACCT TCATGCACCA GGAGTGTGAA
    GCCATCGTCC AGTCCATCAT TCACATCCGG ACACGCTGGG AGCTGTCACA GCCCGACTCC
    ATCCCCCAGC ACACCAAGAT CCGGCCAAAA GATGTCCCTG GGACACTGCT CAATATCGCG
    TTACTTAATT TAGGCAGTTC AGACCCTAGT TTACGGTCAG CTGCCTATAA TCTTCTGTGT
    GCCTTAACTT GTACCTTTAA TTTAAAAATC GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA
    TGTATCCCTG CCAACAACAC CCTCTTTATT GTCTCTATTA GTAAGACACT GGCAGCCAAT
    GAGCCACACC TCACCTTAGA ATTTTTGGAA GAGTGTATTT CTGGATTTAG CAAATCTAGT
    ATTGAACTGA AACACCTTTG TTTGGAATAC ATGACTCCAT GGCTGTCAAA TCTAGTCCGT
    TTTTGTAAGC ACAATGATGA TGCCAAAAGA CAAAGAGTTA CTGCTATTCT TGATAAGCTG
    ATAACAATGA CCATAAATGA GAAACAGATG TACCCATCTA TTCAAGCAAA AATATGGGGG
    AGCCTTGGGC AGATTACAGA TCTCCTTGAT GTTGTGCTAG ACAGTTTCAT CAAAACCAGT
    GCAACAGGTG GCTTGGGATC AATAAAAGCC GAGGTGATGG CAGATACTGC TGTAGCTCTG
    GCGTCTGGAA ATGTGAAATT GGTTTCTAGC AAGGTTATTG GAAGGATGTG CAAAATAATT
    GACAAGACAT GCTTATCTCC AACTCCTACT TTAGAACAGC ATCTTATGTG GGATGACATT
    GCGATTCTAG CGCGCTACAT GCTGATGTTG TCCTTCAACA ACTCCCTCGA CGTGGCGGCT
    CATCTCCCCT ACCTCTTCCA CGTTGTGACT TTCTTAGTAG CCACAGGGCC ACTCTCCCTT
    AGAGCTTCCA CACATGGATT GGTCATTAAT ATCATTCACT CTCTGTGCAC TTGTTCACAG
    CTTCATTTTA GTGAGGAGAC CAAGCAAGTT TTGAGACTCA GTTTGACAGA GTTCTCATTA
    CCCAAATTTT ACTTACTGTT TGGCATTAGC AAAGTCAAGT CAGCTGCTGT GATTGCCTTC
    CGCTCCAGTT ACCGGGACAG GTCGTTCTCT CCTGGCTCCT ACGAGAGGGA GACTTTTGCT
    TTGACATCCT TGGAAACAGT CACGGAAGCT TTGTTGGAGA TCATGGAGGC ATGTATGAGA
    GATATTCCAA CATGCAAGTG GCTGGATCAG TGGACAGAAC TAGCTCAAAG GTTTGCATTC
    CAGTACAACC CGTCCCTGCA ACCGAGAGCT CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA
    GTGTCTCACG GGCAGATAAA GCAGATTATC CGCATTCTTA GCAAGGCGCT TGAGAGTTGC
    TTAAAAGGAC CTGATACTTA CAACAGTCAA GTTCTGATAG AAGCTACAGT AATAGCACTA
    ACCAAATTAC AACCACTTCT TAATAAGGAC TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA
    GCTGTGGCCG TGCTGCAGCT CGATGAGGTC AACTTGTATT CAGCAGGCAC CGCACTTCTT
    GAGCAAAACC TGCACACCTT AGACAGTCTC CGGATATTCA ATGACAAGAG TCCAGAGGAA
    GTATTTATGG CAATTCGGAA TCCTCTGGAA TGGCACTGCA AGCAAATGGA TCATTTTGTT
    GGACTCAATT TCAACTCTAA CTTTAACTTT GCACTGGTTG GACACCTCTT AAAAGGGTAC
    AGACATCCTT CACCTGCTAT TGTTGCACGA ACAGTCAGAA TTTTGCATAC ACTACTAACT
    CTGGTTAACA AACATAGAAA TTGTGACAAA TTTGAGGTGA ATACGCAGAG CGTGGCTTAC
    TTAGCAGCTT TACTCACAGT ATCTGAGGAG GTTCGAAGTC GCTGTAGCCT AAAACATAGA
    AAGTCTCTTC TTCTTACTGA TATTTCAATG GAAAATATTC CTATGGATAC ATATCCCATT
    CATCATGGCG ACCCCAGCTA TAGGACACTA AAGGAGAGTC AGCCATGGTC CTCTCCCAAA
    GGCTCGGAAG GGTACCTTGC AGCCACCTAT CCAACCGTGG GCCAGACCAG CCCCCGAGCC
    CGGAAATCCA TGAGCTTGGA CATGGGGCAG CCTTCTCAGG CCAACACTAA GAAGTTGCTG
    GGAACAAGGA AAAGTTTTGA TCACTTGATA TCAGACACGA AGGCTCCTAA AAGGCAAGAA
    GTGGAATCAG GGATCACAAC ACCCCCCAAA ATGAGGAGAG TAGCAGAAAC TGATTATGAA
    ATGGAAACTC AAAGGATTTC CTCATCACAA CAGCACCCAC ATTTACGTAA AGTTTCAGTG
    TCTGAATCAA ATGTTCTCTT GGATGAAGAA GTACTTACTG ACCCGAAGAT CCAGGCATTG
    CTGCTTACTG TTCTGGCTAC ACTGGTGAAA TACACCACGG ATGAGTTTGA TCAACGAATT
    CTTTATGAAT ACTTAGCAGA AGCCAGTGTT GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC
    AATTTGTTGG ACTCCAAGAT CAACACCCTG TTGTCATTGT GCCAAGATCC AAATTTGTTA
    AATCCGATTC ATGGGATTGT GCAGAGTGTG GTATACTACG AGGAGTCCCC ACCACAGTAC
    CAGACGTCTT ACCTGCAGAG TTTTGGTTTT AATGGCTTGT GGCGGTTTGC AGGACCGTTT
    TCAAAGCAAA CACAAATCCC AGACTATGCT GAGCTTATTG TTAAGTTTCT TGATGCCTTG
    ATTGACACGT ATTTGCCTGG AATTGATGAA GAAACCAGTG AGGAGTCCCT CCTGACACCC
    ACATCTCCTT ATCCTCCTGC AGTGCAGAGC CAGCTTAGTA TTACTGCTAA CCTTAACCTT
    TCCAATTCCA TGACCTCACT TGCAACTTCC CAGCATTCCC CAGGAATCGA CAAGGAGAAC
    GTTGAACTCT CCCCAACCAC TGGGCACTGT AACAGTGGAC GAACTCGCCA TGGATCCGCA
    AGCCAAGTGC AGAAGCAAAG AAGCGCTGGC AGTTTCAAAC GTAATAGCAT TAAGAAGATC
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45488
    Clone ID Related Accession (Same CDS sequence) XM_023653060.1
    Accession Version XM_023653060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGTGG TTAGCCAGCG TTTCCCTCAG AACAGCATCG GTGCAGTGGG AAGTGCCATG
    TTCCTCAGAT TCATCAATCC TGCCATCGTC TCACCGTATG AAGCAGGCAT TTTAGATAAA
    AAGCCACCAC CTAGAATCGA AAGGGGCTTG AAGTTAATGT CAAAGATACT TCAGAGTATT
    GCCAATCATG TTCTCTTCAC AAAAGAAGAG CATATGCGGC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ATGCAGCACG AAGGTTTTTC CTTGATATAG CATCCGATTG TCCTACAAGT
    GATGCAGTGA ATCACAGTCT TTCCTTCATC AGCGATGGCA ACGTGCTGGC TCTACATCGT
    CTCCTCTGGA ACAATCAAGA GAAGATTGGC CAGTATCTTT CCAGCAATAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCAACACTTC TTGCATACCT GGGTCCTCCA
    GAGCACAAAC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTC
    GAGGAATTTA TGACTAGGCA TCAAGTACAT GAAAAAGAAG AGTTCAAGGC CTTGAAAACA
    TTAAGTATTT TCTACCAAGC TGGGACTTCC AGAGCTGGGA ATCCTATATT TTATTATGTT
    GCACGGAGGT TCAAAACTGG TCAAATCAAT GGCGATTTGC TGATCTACCA TGTCTTGCTG
    ACTCTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACCGGG
    CCTAGCAATC GCTTTAAAAC GGACTTTCTC TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT
    GCTTATGACA ATGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAC
    ACCAAGTATC ACGAGCGGCT GCTCACTGGC CTCAAAGGCA GCAAAAGGCT CATTTTCATA
    GACTGTCCTG GGAAGCTGGC TGAGCACGTA GAGCACGAAC AACAGAAACT ACCTGCTGCC
    ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG TTCCACAATG CTCTCAAGCT GGCTCACAAA
    GACACCAAGG TTTCCATTAA AGTTGGTTCT ACTGCTGTTC AAGTGACCTC AGCAGAGCGA
    ACGAAAGTCC TAGGGCAATC AGTCTTTCTA AACGATATCT ACTATGCTTC GGAAATTGAA
    GAGATCTGCC TGGTGGATGA GAACCAGTTC ACCTTAACCA TTGCAAACCA GGGCACACCG
    CTCACCTTCA TGCACCAGGA GTGTGAAGCC ATCGTCCAGT CCATCATTCA CATCCGGACA
    CGCTGGGAGC TGTCACAGCC CGACTCCATC CCCCAGCACA CCAAGATCCG GCCAAAAGAT
    GTCCCTGGGA CACTGCTCAA TATCGCGTTA CTTAATTTAG GCAGTTCAGA CCCTAGTTTA
    CGGTCAGCTG CCTATAATCT TCTGTGTGCC TTAACTTGTA CCTTTAATTT AAAAATCGAG
    GGCCAGTTGC TAGAGACGTC AGGTTTATGT ATCCCTGCCA ACAACACCCT CTTTATTGTC
    TCTATTAGTA AGACACTGGC AGCCAATGAG CCACACCTCA CCTTAGAATT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAACTGAAAC ACCTTTGTTT GGAATACATG
    ACTCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCACA ATGATGATGC CAAAAGACAA
    AGAGTTACTG CTATTCTTGA TAAGCTGATA ACAATGACCA TAAATGAGAA ACAGATGTAC
    CCATCTATTC AAGCAAAAAT ATGGGGGAGC CTTGGGCAGA TTACAGATCT CCTTGATGTT
    GTGCTAGACA GTTTCATCAA AACCAGTGCA ACAGGTGGCT TGGGATCAAT AAAAGCCGAG
    GTGATGGCAG ATACTGCTGT AGCTCTGGCG TCTGGAAATG TGAAATTGGT TTCTAGCAAG
    GTTATTGGAA GGATGTGCAA AATAATTGAC AAGACATGCT TATCTCCAAC TCCTACTTTA
    GAACAGCATC TTATGTGGGA TGACATTGCG ATTCTAGCGC GCTACATGCT GATGTTGTCC
    TTCAACAACT CCCTCGACGT GGCGGCTCAT CTCCCCTACC TCTTCCACGT TGTGACTTTC
    TTAGTAGCCA CAGGGCCACT CTCCCTTAGA GCTTCCACAC ATGGATTGGT CATTAATATC
    ATTCACTCTC TGTGCACTTG TTCACAGCTT CATTTTAGTG AGGAGACCAA GCAAGTTTTG
    AGACTCAGTT TGACAGAGTT CTCATTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC TCCAGTTACC GGGACAGGTC GTTCTCTCCT
    GGCTCCTACG AGAGGGAGAC TTTTGCTTTG ACATCCTTGG AAACAGTCAC GGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGGTT TGCATTCCAG TACAACCCGT CCCTGCAACC GAGAGCTCTG
    GTCGTCTTTG GCTGCATTAG TAAGCGAGTG TCTCACGGGC AGATAAAGCA GATTATCCGC
    ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA AAAGGACCTG ATACTTACAA CAGTCAAGTT
    CTGATAGAAG CTACAGTAAT AGCACTAACC AAATTACAAC CACTTCTTAA TAAGGACTCC
    CCTCTGCACA AAGCCCTCTT TTGGGTAGCT GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC
    TTGTATTCAG CAGGCACCGC ACTTCTTGAG CAAAACCTGC ACACCTTAGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC AGAGGAAGTA TTTATGGCAA TTCGGAATCC TCTGGAATGG
    CACTGCAAGC AAATGGATCA TTTTGTTGGA CTCAATTTCA ACTCTAACTT TAACTTTGCA
    CTGGTTGGAC ACCTCTTAAA AGGGTACAGA CATCCTTCAC CTGCTATTGT TGCACGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTG GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CGCAGAGCGT GGCTTACTTA GCAGCTTTAC TCACAGTATC TGAGGAGGTT
    CGAAGTCGCT GTAGCCTAAA ACATAGAAAG TCTCTTCTTC TTACTGATAT TTCAATGGAA
    AATATTCCTA TGGATACATA TCCCATTCAT CATGGCGACC CCAGCTATAG GACACTAAAG
    GAGAGTCAGC CATGGTCCTC TCCCAAAGGC TCGGAAGGGT ACCTTGCAGC CACCTATCCA
    ACCGTGGGCC AGACCAGCCC CCGAGCCCGG AAATCCATGA GCTTGGACAT GGGGCAGCCT
    TCTCAGGCCA ACACTAAGAA GTTGCTGGGA ACAAGGAAAA GTTTTGATCA CTTGATATCA
    GACACGAAGG CTCCTAAAAG GCAAGAAGTG GAATCAGGGA TCACAACACC CCCCAAAATG
    AGGAGAGTAG CAGAAACTGA TTATGAAATG GAAACTCAAA GGATTTCCTC ATCACAACAG
    CACCCACATT TACGTAAAGT TTCAGTGTCT GAATCAAATG TTCTCTTGGA TGAAGAAGTA
    CTTACTGACC CGAAGATCCA GGCATTGCTG CTTACTGTTC TGGCTACACT GGTGAAATAC
    ACCACGGATG AGTTTGATCA ACGAATTCTT TATGAATACT TAGCAGAAGC CAGTGTTGTT
    TTCCCCAAAG TTTTTCCTGT TGTGCACAAT TTGTTGGACT CCAAGATCAA CACCCTGTTG
    TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCGATTCATG GGATTGTGCA GAGTGTGGTA
    TACTACGAGG AGTCCCCACC ACAGTACCAG ACGTCTTACC TGCAGAGTTT TGGTTTTAAT
    GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG
    CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTATT TGCCTGGAAT TGATGAAGAA
    ACCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTATC CTCCTGCAGT GCAGAGCCAG
    CTTAGTATTA CTGCTAACCT TAACCTTTCC AATTCCATGA CCTCACTTGC AACTTCCCAG
    CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CAACCACTGG GCACTGTAAC
    AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT
    TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508828.1
    CDS165..8657
    Translation

    Target ORF information:

    RefSeq Version XM_023653060.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGTGG TTAGCCAGCG TTTCCCTCAG AACAGCATCG GTGCAGTGGG AAGTGCCATG
    TTCCTCAGAT TCATCAATCC TGCCATCGTC TCACCGTATG AAGCAGGCAT TTTAGATAAA
    AAGCCACCAC CTAGAATCGA AAGGGGCTTG AAGTTAATGT CAAAGATACT TCAGAGTATT
    GCCAATCATG TTCTCTTCAC AAAAGAAGAG CATATGCGGC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ATGCAGCACG AAGGTTTTTC CTTGATATAG CATCCGATTG TCCTACAAGT
    GATGCAGTGA ATCACAGTCT TTCCTTCATC AGCGATGGCA ACGTGCTGGC TCTACATCGT
    CTCCTCTGGA ACAATCAAGA GAAGATTGGC CAGTATCTTT CCAGCAATAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCAACACTTC TTGCATACCT GGGTCCTCCA
    GAGCACAAAC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTC
    GAGGAATTTA TGACTAGGCA TCAAGTACAT GAAAAAGAAG AGTTCAAGGC CTTGAAAACA
    TTAAGTATTT TCTACCAAGC TGGGACTTCC AGAGCTGGGA ATCCTATATT TTATTATGTT
    GCACGGAGGT TCAAAACTGG TCAAATCAAT GGCGATTTGC TGATCTACCA TGTCTTGCTG
    ACTCTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACCGGG
    CCTAGCAATC GCTTTAAAAC GGACTTTCTC TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT
    GCTTATGACA ATGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAC
    ACCAAGTATC ACGAGCGGCT GCTCACTGGC CTCAAAGGCA GCAAAAGGCT CATTTTCATA
    GACTGTCCTG GGAAGCTGGC TGAGCACGTA GAGCACGAAC AACAGAAACT ACCTGCTGCC
    ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG TTCCACAATG CTCTCAAGCT GGCTCACAAA
    GACACCAAGG TTTCCATTAA AGTTGGTTCT ACTGCTGTTC AAGTGACCTC AGCAGAGCGA
    ACGAAAGTCC TAGGGCAATC AGTCTTTCTA AACGATATCT ACTATGCTTC GGAAATTGAA
    GAGATCTGCC TGGTGGATGA GAACCAGTTC ACCTTAACCA TTGCAAACCA GGGCACACCG
    CTCACCTTCA TGCACCAGGA GTGTGAAGCC ATCGTCCAGT CCATCATTCA CATCCGGACA
    CGCTGGGAGC TGTCACAGCC CGACTCCATC CCCCAGCACA CCAAGATCCG GCCAAAAGAT
    GTCCCTGGGA CACTGCTCAA TATCGCGTTA CTTAATTTAG GCAGTTCAGA CCCTAGTTTA
    CGGTCAGCTG CCTATAATCT TCTGTGTGCC TTAACTTGTA CCTTTAATTT AAAAATCGAG
    GGCCAGTTGC TAGAGACGTC AGGTTTATGT ATCCCTGCCA ACAACACCCT CTTTATTGTC
    TCTATTAGTA AGACACTGGC AGCCAATGAG CCACACCTCA CCTTAGAATT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAACTGAAAC ACCTTTGTTT GGAATACATG
    ACTCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCACA ATGATGATGC CAAAAGACAA
    AGAGTTACTG CTATTCTTGA TAAGCTGATA ACAATGACCA TAAATGAGAA ACAGATGTAC
    CCATCTATTC AAGCAAAAAT ATGGGGGAGC CTTGGGCAGA TTACAGATCT CCTTGATGTT
    GTGCTAGACA GTTTCATCAA AACCAGTGCA ACAGGTGGCT TGGGATCAAT AAAAGCCGAG
    GTGATGGCAG ATACTGCTGT AGCTCTGGCG TCTGGAAATG TGAAATTGGT TTCTAGCAAG
    GTTATTGGAA GGATGTGCAA AATAATTGAC AAGACATGCT TATCTCCAAC TCCTACTTTA
    GAACAGCATC TTATGTGGGA TGACATTGCG ATTCTAGCGC GCTACATGCT GATGTTGTCC
    TTCAACAACT CCCTCGACGT GGCGGCTCAT CTCCCCTACC TCTTCCACGT TGTGACTTTC
    TTAGTAGCCA CAGGGCCACT CTCCCTTAGA GCTTCCACAC ATGGATTGGT CATTAATATC
    ATTCACTCTC TGTGCACTTG TTCACAGCTT CATTTTAGTG AGGAGACCAA GCAAGTTTTG
    AGACTCAGTT TGACAGAGTT CTCATTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC TCCAGTTACC GGGACAGGTC GTTCTCTCCT
    GGCTCCTACG AGAGGGAGAC TTTTGCTTTG ACATCCTTGG AAACAGTCAC GGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGGTT TGCATTCCAG TACAACCCGT CCCTGCAACC GAGAGCTCTG
    GTCGTCTTTG GCTGCATTAG TAAGCGAGTG TCTCACGGGC AGATAAAGCA GATTATCCGC
    ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA AAAGGACCTG ATACTTACAA CAGTCAAGTT
    CTGATAGAAG CTACAGTAAT AGCACTAACC AAATTACAAC CACTTCTTAA TAAGGACTCC
    CCTCTGCACA AAGCCCTCTT TTGGGTAGCT GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC
    TTGTATTCAG CAGGCACCGC ACTTCTTGAG CAAAACCTGC ACACCTTAGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC AGAGGAAGTA TTTATGGCAA TTCGGAATCC TCTGGAATGG
    CACTGCAAGC AAATGGATCA TTTTGTTGGA CTCAATTTCA ACTCTAACTT TAACTTTGCA
    CTGGTTGGAC ACCTCTTAAA AGGGTACAGA CATCCTTCAC CTGCTATTGT TGCACGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTG GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CGCAGAGCGT GGCTTACTTA GCAGCTTTAC TCACAGTATC TGAGGAGGTT
    CGAAGTCGCT GTAGCCTAAA ACATAGAAAG TCTCTTCTTC TTACTGATAT TTCAATGGAA
    AATATTCCTA TGGATACATA TCCCATTCAT CATGGCGACC CCAGCTATAG GACACTAAAG
    GAGAGTCAGC CATGGTCCTC TCCCAAAGGC TCGGAAGGGT ACCTTGCAGC CACCTATCCA
    ACCGTGGGCC AGACCAGCCC CCGAGCCCGG AAATCCATGA GCTTGGACAT GGGGCAGCCT
    TCTCAGGCCA ACACTAAGAA GTTGCTGGGA ACAAGGAAAA GTTTTGATCA CTTGATATCA
    GACACGAAGG CTCCTAAAAG GCAAGAAGTG GAATCAGGGA TCACAACACC CCCCAAAATG
    AGGAGAGTAG CAGAAACTGA TTATGAAATG GAAACTCAAA GGATTTCCTC ATCACAACAG
    CACCCACATT TACGTAAAGT TTCAGTGTCT GAATCAAATG TTCTCTTGGA TGAAGAAGTA
    CTTACTGACC CGAAGATCCA GGCATTGCTG CTTACTGTTC TGGCTACACT GGTGAAATAC
    ACCACGGATG AGTTTGATCA ACGAATTCTT TATGAATACT TAGCAGAAGC CAGTGTTGTT
    TTCCCCAAAG TTTTTCCTGT TGTGCACAAT TTGTTGGACT CCAAGATCAA CACCCTGTTG
    TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCGATTCATG GGATTGTGCA GAGTGTGGTA
    TACTACGAGG AGTCCCCACC ACAGTACCAG ACGTCTTACC TGCAGAGTTT TGGTTTTAAT
    GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG
    CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTATT TGCCTGGAAT TGATGAAGAA
    ACCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTATC CTCCTGCAGT GCAGAGCCAG
    CTTAGTATTA CTGCTAACCT TAACCTTTCC AATTCCATGA CCTCACTTGC AACTTCCCAG
    CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CAACCACTGG GCACTGTAAC
    AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT
    TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45489
    Clone ID Related Accession (Same CDS sequence) XM_023653061.1
    Accession Version XM_023653061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2521
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    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
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    3421
    3481
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    3721
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    4141
    4201
    4261
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    4381
    4441
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    4681
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    4981
    5041
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    5161
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    7141
    7201
    7261
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    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCACTGG ATTGGTGGCC TAAGATCGAT GCCGTATATT GTCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCG GTGCAGGGTT GTGGAGCACA CCCGGCGATA
    CGCATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTTT TGTCTATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCCAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTGCTTCATC AGTTAGACAG CATCGATTTG
    TGGAATCCTG ATGCTCCTGT GGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AGTTAACTAG CCAGCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGGAGT
    TCCTGTCACT TTCTCTTCCT TTATGGGGTA GGATGTGATG TTCCTTCCAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAGTTA TTACGTACTT ATACTCCTGG AGCCTCTCTC
    CGGAAGGGAA AAGGAAACTC ATCCATGGAT AGTGCGGCAG GGTGCAGTGG AACGCCGCCG
    ATATGCCGGC AAGCCCAGAC CAAACTGGAA GTGGCCCTGT ACATGTTTCT GTGGAGCCCT
    GACACCGAAG CTGTTCTGGT TGCCATGTCC TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT
    ATCCGGTGTG GGGTGGACGA AGTATCGGTG CACAACTTCT TGCCGAACTA TAACACATTC
    ATGGAATTTG CGTCTGTCAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCAC TACTGAGGCG CATTGAACAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACACATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CATAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTG TCTGACACGG ACTCCCTTCA GGAATGGATC
    AACATGACCG GCTTCCTCTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCCTGGCAA CCTATAGCCC ACCCATGGGC CCAGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CCTCAGAGGG AAATGCAGAC ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTATCCTTAA TGGTGTGTAA CCATGAGAAA GTGGGACTTC AAATACGCAC CAACGTTAAG
    GATCTGGTGG GTTTAGAATT GAGTCCTGCT CTTTATCCAA TGCTGTTTAA CAAATTGAAG
    AACACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAATTTGTAG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACTGAAGGC
    AGCTCCGAAC ATCTGGGGCA AGCTAGCATT GAAACGATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGGAACAT GGTCCATGCA ATTCAAATAA AAACGAAACT GTGTCAGCTA
    GTCGAGGTAA TGATGGCAAG AAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT GACAGATTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGATGTAA AATGTCTTAC AAGAGATCTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAACT CATGGAAGCC
    AAGTCACAGC TATTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAGC GTGGCATGTC TCGGAGGCTG
    GCCTCACTGA GGCACTGCAC AGTCCTTGCA ATGTCAAACT TACTTAACGC TAACGTGGAC
    AGTGGCCTCA TGCACTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACA
    TTTATGGAAG TTCTGACAAA AATCCTCCAA CAAGGCACAG AATTTGACAC ACTTGCAGAA
    ACGGTGTTGG CAGATCGGTT TGAGAGGCTG GTGGAGTTGG TCACAATGAT GGGTGATCAG
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAA
    CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT TCTCGGCATT TACTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCCATGC AGACTCTTTT CCGAGGCAAC
    AGCTTAGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAA
    AAACTCCTGG ATCCTTTGTT ACGAATTGTG ATCACATCCT CTGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC
    CTCCTTCAGA TGACTGAAAA GTTCTTCCAC GCCATCATCA GTTCCTCCTC GGAATTCCCC
    CCTCAGCTTC GAAGTGTGTG CCACTGTCTA TACCAGGTGG TTAGCCAGCG TTTCCCTCAG
    AACAGCATCG GTGCAGTGGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATCGTC
    TCACCGTATG AAGCAGGCAT TTTAGATAAA AAGCCACCAC CTAGAATCGA AAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TTCTCTTCAC AAAAGAAGAG
    CATATGCGGC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ATGCAGCACG AAGGTTTTTC
    CTTGATATAG CATCCGATTG TCCTACAAGT GATGCAGTGA ATCACAGTCT TTCCTTCATC
    AGCGATGGCA ACGTGCTGGC TCTACATCGT CTCCTCTGGA ACAATCAAGA GAAGATTGGC
    CAGTATCTTT CCAGCAATAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCAACACTTC TTGCATACCT GGGTCCTCCA GAGCACAAAC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTC GAGGAATTTA TGACTAGGCA TCAAGTACAT
    GAAAAAGAAG AGTTCAAGGC CTTGAAAACA TTAAGTATTT TCTACCAAGC TGGGACTTCC
    AGAGCTGGGA ATCCTATATT TTATTATGTT GCACGGAGGT TCAAAACTGG TCAAATCAAT
    GGCGATTTGC TGATCTACCA TGTCTTGCTG ACTCTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACCGGG CCTAGCAATC GCTTTAAAAC GGACTTTCTC
    TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT GCTTATGACA ATGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAC ACCAAGTATC ACGAGCGGCT GCTCACTGGC
    CTCAAAGGCA GCAAAAGGCT CATTTTCATA GACTGTCCTG GGAAGCTGGC TGAGCACGTA
    GAGCACGAAC AACAGAAACT ACCTGCTGCC ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG
    TTCCACAATG CTCTCAAGCT GGCTCACAAA GACACCAAGG TTTCCATTAA AGTTGGTTCT
    ACTGCTGTTC AAGTGACCTC AGCAGAGCGA ACGAAAGTCC TAGGGCAATC AGTCTTTCTA
    AACGATATCT ACTATGCTTC GGAAATTGAA GAGATCTGCC TGGTGGATGA GAACCAGTTC
    ACCTTAACCA TTGCAAACCA GGGCACACCG CTCACCTTCA TGCACCAGGA GTGTGAAGCC
    ATCGTCCAGT CCATCATTCA CATCCGGACA CGCTGGGAGC TGTCACAGCC CGACTCCATC
    CCCCAGCACA CCAAGATCCG GCCAAAAGAT GTCCCTGGGA CACTGCTCAA TATCGCGTTA
    CTTAATTTAG GCAGTTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACTTGTA CCTTTAATTT AAAAATCGAG GGCCAGTTGC TAGAGACGTC AGGTTTATGT
    ATCCCTGCCA ACAACACCCT CTTTATTGTC TCTATTAGTA AGACACTGGC AGCCAATGAG
    CCACACCTCA CCTTAGAATT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAACTGAAAC ACCTTTGTTT GGAATACATG ACTCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCACA ATGATGATGC CAAAAGACAA AGAGTTACTG CTATTCTTGA TAAGCTGATA
    ACAATGACCA TAAATGAGAA ACAGATGTAC CCATCTATTC AAGCAAAAAT ATGGGGGAGC
    CTTGGGCAGA TTACAGATCT CCTTGATGTT GTGCTAGACA GTTTCATCAA AACCAGTGCA
    ACAGGTGGCT TGGGATCAAT AAAAGCCGAG GTGATGGCAG ATACTGCTGT AGCTCTGGCG
    TCTGGAAATG TGAAATTGGT TTCTAGCAAG GTTATTGGAA GGATGTGCAA AATAATTGAC
    AAGACATGCT TATCTCCAAC TCCTACTTTA GAACAGCATC TTATGTGGGA TGACATTGCG
    ATTCTAGCGC GCTACATGCT GATGTTGTCC TTCAACAACT CCCTCGACGT GGCGGCTCAT
    CTCCCCTACC TCTTCCACGT TGTGACTTTC TTAGTAGCCA CAGGGCCACT CTCCCTTAGA
    GCTTCCACAC ATGGATTGGT CATTAATATC ATTCACTCTC TGTGCACTTG TTCACAGCTT
    CATTTTAGTG AGGAGACCAA GCAAGTTTTG AGACTCAGTT TGACAGAGTT CTCATTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC
    TCCAGTTACC GGGACAGGTC GTTCTCTCCT GGCTCCTACG AGAGGGAGAC TTTTGCTTTG
    ACATCCTTGG AAACAGTCAC GGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGGTT TGCATTCCAG
    TACAACCCGT CCCTGCAACC GAGAGCTCTG GTCGTCTTTG GCTGCATTAG TAAGCGAGTG
    TCTCACGGGC AGATAAAGCA GATTATCCGC ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA
    AAAGGACCTG ATACTTACAA CAGTCAAGTT CTGATAGAAG CTACAGTAAT AGCACTAACC
    AAATTACAAC CACTTCTTAA TAAGGACTCC CCTCTGCACA AAGCCCTCTT TTGGGTAGCT
    GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC TTGTATTCAG CAGGCACCGC ACTTCTTGAG
    CAAAACCTGC ACACCTTAGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC AGAGGAAGTA
    TTTATGGCAA TTCGGAATCC TCTGGAATGG CACTGCAAGC AAATGGATCA TTTTGTTGGA
    CTCAATTTCA ACTCTAACTT TAACTTTGCA CTGGTTGGAC ACCTCTTAAA AGGGTACAGA
    CATCCTTCAC CTGCTATTGT TGCACGAACA GTCAGAATTT TGCATACACT ACTAACTCTG
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CGCAGAGCGT GGCTTACTTA
    GCAGCTTTAC TCACAGTATC TGAGGAGGTT CGAAGTCGCT GTAGCCTAAA ACATAGAAAG
    TCTCTTCTTC TTACTGATAT TTCAATGGAA AATATTCCTA TGGATACATA TCCCATTCAT
    CATGGCGACC CCAGCTATAG GACACTAAAG GAGAGTCAGC CATGGTCCTC TCCCAAAGGC
    TCGGAAGGGT ACCTTGCAGC CACCTATCCA ACCGTGGGCC AGACCAGCCC CCGAGCCCGG
    AAATCCATGA GCTTGGACAT GGGGCAGCCT TCTCAGGCCA ACACTAAGAA GTTGCTGGGA
    ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACGAAGG CTCCTAAAAG GCAAGAAGTG
    GAATCAGGGA TCACAACACC CCCCAAAATG AGGAGAGTAG CAGAAACTGA TTATGAAATG
    GAAACTCAAA GGATTTCCTC ATCACAACAG CACCCACATT TACGTAAAGT TTCAGTGTCT
    GAATCAAATG TTCTCTTGGA TGAAGAAGTA CTTACTGACC CGAAGATCCA GGCATTGCTG
    CTTACTGTTC TGGCTACACT GGTGAAATAC ACCACGGATG AGTTTGATCA ACGAATTCTT
    TATGAATACT TAGCAGAAGC CAGTGTTGTT TTCCCCAAAG TTTTTCCTGT TGTGCACAAT
    TTGTTGGACT CCAAGATCAA CACCCTGTTG TCATTGTGCC AAGATCCAAA TTTGTTAAAT
    CCGATTCATG GGATTGTGCA GAGTGTGGTA TACTACGAGG AGTCCCCACC ACAGTACCAG
    ACGTCTTACC TGCAGAGTTT TGGTTTTAAT GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA
    AAGCAAACAC AAATCCCAGA CTATGCTGAG CTTATTGTTA AGTTTCTTGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTATC CTCCTGCAGT GCAGAGCCAG CTTAGTATTA CTGCTAACCT TAACCTTTCC
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAACGTT
    GAACTCTCCC CAACCACTGG GCACTGTAAC AGTGGACGAA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508829.1
    CDS1..8463
    Translation

    Target ORF information:

    RefSeq Version XM_023653061.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCTGCACTGG ATTGGTGGCC TAAGATCGAT GCCGTATATT GTCACTCAGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCG GTGCAGGGTT GTGGAGCACA CCCGGCGATA
    CGCATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTTT TGTCTATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCCAGAAAAC AGGGGCCTGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAGCTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTGCTTCATC AGTTAGACAG CATCGATTTG
    TGGAATCCTG ATGCTCCTGT GGAAACATTT TGGGAAATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AGTTAACTAG CCAGCAAATG CTTAGTAGCA CAGAAATTCT CAAATGGTTG
    CGGGAAATTT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGGAGT
    TCCTGTCACT TTCTCTTCCT TTATGGGGTA GGATGTGATG TTCCTTCCAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAGTTA TTACGTACTT ATACTCCTGG AGCCTCTCTC
    CGGAAGGGAA AAGGAAACTC ATCCATGGAT AGTGCGGCAG GGTGCAGTGG AACGCCGCCG
    ATATGCCGGC AAGCCCAGAC CAAACTGGAA GTGGCCCTGT ACATGTTTCT GTGGAGCCCT
    GACACCGAAG CTGTTCTGGT TGCCATGTCC TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT
    ATCCGGTGTG GGGTGGACGA AGTATCGGTG CACAACTTCT TGCCGAACTA TAACACATTC
    ATGGAATTTG CGTCTGTCAG CAATATGATG TCAACAGGAA GAGCAGCACT TCAGAAAAGA
    GTGATGGCAC TACTGAGGCG CATTGAACAT CCCACTGCAG GAAACACTGA GGCTTGGGAA
    GATACACATG CAAAGTGGGA ACAAGCTACA AAACTAATCC TCAACTATCC AAAAGCCAAA
    ATGGAAGATG GCCAGGCTGC TGAAAGCCTT CATAAGACCA TTGTTAAGAG GCGAATGTCT
    CATGTGAGTG GAGGAGGATC CATAGATTTG TCTGACACGG ACTCCCTTCA GGAATGGATC
    AACATGACCG GCTTCCTCTG TGCCCTTGGG GGAGTGTGCC TGCAGCAGAG AAGCAATTCT
    GGCCTGGCAA CCTATAGCCC ACCCATGGGC CCAGTCAGTG AACGCAAGGG TTCCATGATT
    TCAGTGATGT CCTCAGAGGG AAATGCAGAC ACACCTGTCA GCAAATTTAT GGATCGGCTG
    CTATCCTTAA TGGTGTGTAA CCATGAGAAA GTGGGACTTC AAATACGCAC CAACGTTAAG
    GATCTGGTGG GTTTAGAATT GAGTCCTGCT CTTTATCCAA TGCTGTTTAA CAAATTGAAG
    AACACCATCA GCAAGTTTTT TGACTCCCAA GGACAGGTTT TATTGACTGA CACCAATACT
    CAATTTGTAG AGCAAACCAT AGCTATAATG AAGAACTTAC TAGATAATCA TACTGAAGGC
    AGCTCCGAAC ATCTGGGGCA AGCTAGCATT GAAACGATGA TGTTAAATCT GGTCAGATAT
    GTTCGTGTAC TTGGGAACAT GGTCCATGCA ATTCAAATAA AAACGAAACT GTGTCAGCTA
    GTCGAGGTAA TGATGGCAAG AAGAGATGAC CTCTCATTTT GTCAAGAGAT GAAATTTAGG
    AATAAGATGG TAGAATATCT GACAGATTGG GTTATGGGAA CATCAAACCA AGCAGCAGAT
    GATGATGTAA AATGTCTTAC AAGAGATCTG GACCAGGCAA GCATGGAAGC AGTAGTTTCA
    CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG GAAGGAGATG GTGTGGAACT CATGGAAGCC
    AAGTCACAGC TATTTCTTAA GTATTTCACA TTGTTTATGA ACCTTTTGAA TGACTGTAGT
    GAAGTTGAAG ATGAAAATGC ACAAACAGGT GGCAGGAAGC GTGGCATGTC TCGGAGGCTG
    GCCTCACTGA GGCACTGCAC AGTCCTTGCA ATGTCAAACT TACTTAACGC TAACGTGGAC
    AGTGGCCTCA TGCACTCCAT AGGTTTAGGT TATCACAAAG ATCTCCAGAC AAGAGCTACA
    TTTATGGAAG TTCTGACAAA AATCCTCCAA CAAGGCACAG AATTTGACAC ACTTGCAGAA
    ACGGTGTTGG CAGATCGGTT TGAGAGGCTG GTGGAGTTGG TCACAATGAT GGGTGATCAG
    GGAGAGCTCC CTATAGCCAT GGCTCTGGCC AATGTGGTCC CTTGTTCTCA GTGGGATGAA
    CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT TCTCGGCATT TACTCTACCA ACTGCTCTGG
    AACATGTTTT CTAAGGAAGT AGAATTGGCA GACTCCATGC AGACTCTTTT CCGAGGCAAC
    AGCTTAGCCA GTAAAATAAT GACCTTCTGT TTCAAGGTAT ATGGTGCTAC CTACCTACAA
    AAACTCCTGG ATCCTTTGTT ACGAATTGTG ATCACATCCT CTGATTGGCA ACATGTTAGC
    TTTGAAGTGG ATCCTACCAG GTTAGAACAG TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC
    CTCCTTCAGA TGACTGAAAA GTTCTTCCAC GCCATCATCA GTTCCTCCTC GGAATTCCCC
    CCTCAGCTTC GAAGTGTGTG CCACTGTCTA TACCAGGTGG TTAGCCAGCG TTTCCCTCAG
    AACAGCATCG GTGCAGTGGG AAGTGCCATG TTCCTCAGAT TCATCAATCC TGCCATCGTC
    TCACCGTATG AAGCAGGCAT TTTAGATAAA AAGCCACCAC CTAGAATCGA AAGGGGCTTG
    AAGTTAATGT CAAAGATACT TCAGAGTATT GCCAATCATG TTCTCTTCAC AAAAGAAGAG
    CATATGCGGC CTTTTAATGA TTTTGTGAAA AGCAACTTTG ATGCAGCACG AAGGTTTTTC
    CTTGATATAG CATCCGATTG TCCTACAAGT GATGCAGTGA ATCACAGTCT TTCCTTCATC
    AGCGATGGCA ACGTGCTGGC TCTACATCGT CTCCTCTGGA ACAATCAAGA GAAGATTGGC
    CAGTATCTTT CCAGCAATAG GGATCATAAA GCTGTTGGAA GACGACCTTT TGATAAGATG
    GCAACACTTC TTGCATACCT GGGTCCTCCA GAGCACAAAC CTGTGGCAGA TACACACTGG
    TCCAGCCTTA ACCTTACCAG TTCAAAGTTC GAGGAATTTA TGACTAGGCA TCAAGTACAT
    GAAAAAGAAG AGTTCAAGGC CTTGAAAACA TTAAGTATTT TCTACCAAGC TGGGACTTCC
    AGAGCTGGGA ATCCTATATT TTATTATGTT GCACGGAGGT TCAAAACTGG TCAAATCAAT
    GGCGATTTGC TGATCTACCA TGTCTTGCTG ACTCTAAAGC CATATTATGC AAAGCCATAT
    GAAATTGTAG TGGACCTTAC CCATACCGGG CCTAGCAATC GCTTTAAAAC GGACTTTCTC
    TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT GCTTATGACA ATGTCTCTGC AGTCTATATC
    TATAACTGTA ACTCCTGGGT CAGGGAGTAC ACCAAGTATC ACGAGCGGCT GCTCACTGGC
    CTCAAAGGCA GCAAAAGGCT CATTTTCATA GACTGTCCTG GGAAGCTGGC TGAGCACGTA
    GAGCACGAAC AACAGAAACT ACCTGCTGCC ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG
    TTCCACAATG CTCTCAAGCT GGCTCACAAA GACACCAAGG TTTCCATTAA AGTTGGTTCT
    ACTGCTGTTC AAGTGACCTC AGCAGAGCGA ACGAAAGTCC TAGGGCAATC AGTCTTTCTA
    AACGATATCT ACTATGCTTC GGAAATTGAA GAGATCTGCC TGGTGGATGA GAACCAGTTC
    ACCTTAACCA TTGCAAACCA GGGCACACCG CTCACCTTCA TGCACCAGGA GTGTGAAGCC
    ATCGTCCAGT CCATCATTCA CATCCGGACA CGCTGGGAGC TGTCACAGCC CGACTCCATC
    CCCCAGCACA CCAAGATCCG GCCAAAAGAT GTCCCTGGGA CACTGCTCAA TATCGCGTTA
    CTTAATTTAG GCAGTTCAGA CCCTAGTTTA CGGTCAGCTG CCTATAATCT TCTGTGTGCC
    TTAACTTGTA CCTTTAATTT AAAAATCGAG GGCCAGTTGC TAGAGACGTC AGGTTTATGT
    ATCCCTGCCA ACAACACCCT CTTTATTGTC TCTATTAGTA AGACACTGGC AGCCAATGAG
    CCACACCTCA CCTTAGAATT TTTGGAAGAG TGTATTTCTG GATTTAGCAA ATCTAGTATT
    GAACTGAAAC ACCTTTGTTT GGAATACATG ACTCCATGGC TGTCAAATCT AGTCCGTTTT
    TGTAAGCACA ATGATGATGC CAAAAGACAA AGAGTTACTG CTATTCTTGA TAAGCTGATA
    ACAATGACCA TAAATGAGAA ACAGATGTAC CCATCTATTC AAGCAAAAAT ATGGGGGAGC
    CTTGGGCAGA TTACAGATCT CCTTGATGTT GTGCTAGACA GTTTCATCAA AACCAGTGCA
    ACAGGTGGCT TGGGATCAAT AAAAGCCGAG GTGATGGCAG ATACTGCTGT AGCTCTGGCG
    TCTGGAAATG TGAAATTGGT TTCTAGCAAG GTTATTGGAA GGATGTGCAA AATAATTGAC
    AAGACATGCT TATCTCCAAC TCCTACTTTA GAACAGCATC TTATGTGGGA TGACATTGCG
    ATTCTAGCGC GCTACATGCT GATGTTGTCC TTCAACAACT CCCTCGACGT GGCGGCTCAT
    CTCCCCTACC TCTTCCACGT TGTGACTTTC TTAGTAGCCA CAGGGCCACT CTCCCTTAGA
    GCTTCCACAC ATGGATTGGT CATTAATATC ATTCACTCTC TGTGCACTTG TTCACAGCTT
    CATTTTAGTG AGGAGACCAA GCAAGTTTTG AGACTCAGTT TGACAGAGTT CTCATTACCC
    AAATTTTACT TACTGTTTGG CATTAGCAAA GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC
    TCCAGTTACC GGGACAGGTC GTTCTCTCCT GGCTCCTACG AGAGGGAGAC TTTTGCTTTG
    ACATCCTTGG AAACAGTCAC GGAAGCTTTG TTGGAGATCA TGGAGGCATG TATGAGAGAT
    ATTCCAACAT GCAAGTGGCT GGATCAGTGG ACAGAACTAG CTCAAAGGTT TGCATTCCAG
    TACAACCCGT CCCTGCAACC GAGAGCTCTG GTCGTCTTTG GCTGCATTAG TAAGCGAGTG
    TCTCACGGGC AGATAAAGCA GATTATCCGC ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA
    AAAGGACCTG ATACTTACAA CAGTCAAGTT CTGATAGAAG CTACAGTAAT AGCACTAACC
    AAATTACAAC CACTTCTTAA TAAGGACTCC CCTCTGCACA AAGCCCTCTT TTGGGTAGCT
    GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC TTGTATTCAG CAGGCACCGC ACTTCTTGAG
    CAAAACCTGC ACACCTTAGA CAGTCTCCGG ATATTCAATG ACAAGAGTCC AGAGGAAGTA
    TTTATGGCAA TTCGGAATCC TCTGGAATGG CACTGCAAGC AAATGGATCA TTTTGTTGGA
    CTCAATTTCA ACTCTAACTT TAACTTTGCA CTGGTTGGAC ACCTCTTAAA AGGGTACAGA
    CATCCTTCAC CTGCTATTGT TGCACGAACA GTCAGAATTT TGCATACACT ACTAACTCTG
    GTTAACAAAC ATAGAAATTG TGACAAATTT GAGGTGAATA CGCAGAGCGT GGCTTACTTA
    GCAGCTTTAC TCACAGTATC TGAGGAGGTT CGAAGTCGCT GTAGCCTAAA ACATAGAAAG
    TCTCTTCTTC TTACTGATAT TTCAATGGAA AATATTCCTA TGGATACATA TCCCATTCAT
    CATGGCGACC CCAGCTATAG GACACTAAAG GAGAGTCAGC CATGGTCCTC TCCCAAAGGC
    TCGGAAGGGT ACCTTGCAGC CACCTATCCA ACCGTGGGCC AGACCAGCCC CCGAGCCCGG
    AAATCCATGA GCTTGGACAT GGGGCAGCCT TCTCAGGCCA ACACTAAGAA GTTGCTGGGA
    ACAAGGAAAA GTTTTGATCA CTTGATATCA GACACGAAGG CTCCTAAAAG GCAAGAAGTG
    GAATCAGGGA TCACAACACC CCCCAAAATG AGGAGAGTAG CAGAAACTGA TTATGAAATG
    GAAACTCAAA GGATTTCCTC ATCACAACAG CACCCACATT TACGTAAAGT TTCAGTGTCT
    GAATCAAATG TTCTCTTGGA TGAAGAAGTA CTTACTGACC CGAAGATCCA GGCATTGCTG
    CTTACTGTTC TGGCTACACT GGTGAAATAC ACCACGGATG AGTTTGATCA ACGAATTCTT
    TATGAATACT TAGCAGAAGC CAGTGTTGTT TTCCCCAAAG TTTTTCCTGT TGTGCACAAT
    TTGTTGGACT CCAAGATCAA CACCCTGTTG TCATTGTGCC AAGATCCAAA TTTGTTAAAT
    CCGATTCATG GGATTGTGCA GAGTGTGGTA TACTACGAGG AGTCCCCACC ACAGTACCAG
    ACGTCTTACC TGCAGAGTTT TGGTTTTAAT GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA
    AAGCAAACAC AAATCCCAGA CTATGCTGAG CTTATTGTTA AGTTTCTTGA TGCCTTGATT
    GACACGTATT TGCCTGGAAT TGATGAAGAA ACCAGTGAGG AGTCCCTCCT GACACCCACA
    TCTCCTTATC CTCCTGCAGT GCAGAGCCAG CTTAGTATTA CTGCTAACCT TAACCTTTCC
    AATTCCATGA CCTCACTTGC AACTTCCCAG CATTCCCCAG GAATCGACAA GGAGAACGTT
    GAACTCTCCC CAACCACTGG GCACTGTAAC AGTGGACGAA CTCGCCATGG ATCCGCAAGC
    CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT TTCAAACGTA ATAGCATTAA GAAGATCGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45490
    Clone ID Related Accession (Same CDS sequence) XM_023653062.1
    Accession Version XM_023653062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGGATAG TGCGGCAGGG TGCAGTGGAA CGCCGCCGAT ATGCCGGCAA
    GCCCAGACCA AACTGGAAGT GGCCCTGTAC ATGTTTCTGT GGAGCCCTGA CACCGAAGCT
    GTTCTGGTTG CCATGTCCTG TTTCCGCCAC CTCTGTGAGG AAGCGGATAT CCGGTGTGGG
    GTGGACGAAG TATCGGTGCA CAACTTCTTG CCGAACTATA ACACATTCAT GGAATTTGCG
    TCTGTCAGCA ATATGATGTC AACAGGAAGA GCAGCACTTC AGAAAAGAGT GATGGCACTA
    CTGAGGCGCA TTGAACATCC CACTGCAGGA AACACTGAGG CTTGGGAAGA TACACATGCA
    AAGTGGGAAC AAGCTACAAA ACTAATCCTC AACTATCCAA AAGCCAAAAT GGAAGATGGC
    CAGGCTGCTG AAAGCCTTCA TAAGACCATT GTTAAGAGGC GAATGTCTCA TGTGAGTGGA
    GGAGGATCCA TAGATTTGTC TGACACGGAC TCCCTTCAGG AATGGATCAA CATGACCGGC
    TTCCTCTGTG CCCTTGGGGG AGTGTGCCTG CAGCAGAGAA GCAATTCTGG CCTGGCAACC
    TATAGCCCAC CCATGGGCCC AGTCAGTGAA CGCAAGGGTT CCATGATTTC AGTGATGTCC
    TCAGAGGGAA ATGCAGACAC ACCTGTCAGC AAATTTATGG ATCGGCTGCT ATCCTTAATG
    GTGTGTAACC ATGAGAAAGT GGGACTTCAA ATACGCACCA ACGTTAAGGA TCTGGTGGGT
    TTAGAATTGA GTCCTGCTCT TTATCCAATG CTGTTTAACA AATTGAAGAA CACCATCAGC
    AAGTTTTTTG ACTCCCAAGG ACAGGTTTTA TTGACTGACA CCAATACTCA ATTTGTAGAG
    CAAACCATAG CTATAATGAA GAACTTACTA GATAATCATA CTGAAGGCAG CTCCGAACAT
    CTGGGGCAAG CTAGCATTGA AACGATGATG TTAAATCTGG TCAGATATGT TCGTGTACTT
    GGGAACATGG TCCATGCAAT TCAAATAAAA ACGAAACTGT GTCAGCTAGT CGAGGTAATG
    ATGGCAAGAA GAGATGACCT CTCATTTTGT CAAGAGATGA AATTTAGGAA TAAGATGGTA
    GAATATCTGA CAGATTGGGT TATGGGAACA TCAAACCAAG CAGCAGATGA TGATGTAAAA
    TGTCTTACAA GAGATCTGGA CCAGGCAAGC ATGGAAGCAG TAGTTTCACT CCTGGCTGGT
    CTCCCGCTGC AGCCTGAGGA AGGAGATGGT GTGGAACTCA TGGAAGCCAA GTCACAGCTA
    TTTCTTAAGT ATTTCACATT GTTTATGAAC CTTTTGAATG ACTGTAGTGA AGTTGAAGAT
    GAAAATGCAC AAACAGGTGG CAGGAAGCGT GGCATGTCTC GGAGGCTGGC CTCACTGAGG
    CACTGCACAG TCCTTGCAAT GTCAAACTTA CTTAACGCTA ACGTGGACAG TGGCCTCATG
    CACTCCATAG GTTTAGGTTA TCACAAAGAT CTCCAGACAA GAGCTACATT TATGGAAGTT
    CTGACAAAAA TCCTCCAACA AGGCACAGAA TTTGACACAC TTGCAGAAAC GGTGTTGGCA
    GATCGGTTTG AGAGGCTGGT GGAGTTGGTC ACAATGATGG GTGATCAGGG AGAGCTCCCT
    ATAGCCATGG CTCTGGCCAA TGTGGTCCCT TGTTCTCAGT GGGATGAACT AGCTCGAGTT
    CTAGTGACTC TCTTTGATTC TCGGCATTTA CTCTACCAAC TGCTCTGGAA CATGTTTTCT
    AAGGAAGTAG AATTGGCAGA CTCCATGCAG ACTCTTTTCC GAGGCAACAG CTTAGCCAGT
    AAAATAATGA CCTTCTGTTT CAAGGTATAT GGTGCTACCT ACCTACAAAA ACTCCTGGAT
    CCTTTGTTAC GAATTGTGAT CACATCCTCT GATTGGCAAC ATGTTAGCTT TGAAGTGGAT
    CCTACCAGGT TAGAACAGTC AGAGAGCCTT GAGGAGAACC AGCGGAGCCT CCTTCAGATG
    ACTGAAAAGT TCTTCCACGC CATCATCAGT TCCTCCTCGG AATTCCCCCC TCAGCTTCGA
    AGTGTGTGCC ACTGTCTATA CCAGGCAACT TGCCACTCCC TACTGAATAC AGCTACAGTA
    AAAGAAAAAA AGGAAAACAA AAAATCAGTG GTTAGCCAGC GTTTCCCTCA GAACAGCATC
    GGTGCAGTGG GAAGTGCCAT GTTCCTCAGA TTCATCAATC CTGCCATCGT CTCACCGTAT
    GAAGCAGGCA TTTTAGATAA AAAGCCACCA CCTAGAATCG AAAGGGGCTT GAAGTTAATG
    TCAAAGATAC TTCAGAGTAT TGCCAATCAT GTTCTCTTCA CAAAAGAAGA GCATATGCGG
    CCTTTTAATG ATTTTGTGAA AAGCAACTTT GATGCAGCAC GAAGGTTTTT CCTTGATATA
    GCATCCGATT GTCCTACAAG TGATGCAGTG AATCACAGTC TTTCCTTCAT CAGCGATGGC
    AACGTGCTGG CTCTACATCG TCTCCTCTGG AACAATCAAG AGAAGATTGG CCAGTATCTT
    TCCAGCAATA GGGATCATAA AGCTGTTGGA AGACGACCTT TTGATAAGAT GGCAACACTT
    CTTGCATACC TGGGTCCTCC AGAGCACAAA CCTGTGGCAG ATACACACTG GTCCAGCCTT
    AACCTTACCA GTTCAAAGTT CGAGGAATTT ATGACTAGGC ATCAAGTACA TGAAAAAGAA
    GAGTTCAAGG CCTTGAAAAC ATTAAGTATT TTCTACCAAG CTGGGACTTC CAGAGCTGGG
    AATCCTATAT TTTATTATGT TGCACGGAGG TTCAAAACTG GTCAAATCAA TGGCGATTTG
    CTGATCTACC ATGTCTTGCT GACTCTAAAG CCATATTATG CAAAGCCATA TGAAATTGTA
    GTGGACCTTA CCCATACCGG GCCTAGCAAT CGCTTTAAAA CGGACTTTCT CTCCAAGTGG
    TTTGTTGTTT TTCCTGGCTT TGCTTATGAC AATGTCTCTG CAGTCTATAT CTATAACTGT
    AACTCCTGGG TCAGGGAGTA CACCAAGTAT CACGAGCGGC TGCTCACTGG CCTCAAAGGC
    AGCAAAAGGC TCATTTTCAT AGACTGTCCT GGGAAGCTGG CTGAGCACGT AGAGCACGAA
    CAACAGAAAC TACCTGCTGC CACTTTGGCT TTAGAAGAGG ACCTGAAAGT GTTCCACAAT
    GCTCTCAAGC TGGCTCACAA AGACACCAAG GTTTCCATTA AAGTTGGTTC TACTGCTGTT
    CAAGTGACCT CAGCAGAGCG AACGAAAGTC CTAGGGCAAT CAGTCTTTCT AAACGATATC
    TACTATGCTT CGGAAATTGA AGAGATCTGC CTGGTGGATG AGAACCAGTT CACCTTAACC
    ATTGCAAACC AGGGCACACC GCTCACCTTC ATGCACCAGG AGTGTGAAGC CATCGTCCAG
    TCCATCATTC ACATCCGGAC ACGCTGGGAG CTGTCACAGC CCGACTCCAT CCCCCAGCAC
    ACCAAGATCC GGCCAAAAGA TGTCCCTGGG ACACTGCTCA ATATCGCGTT ACTTAATTTA
    GGCAGTTCAG ACCCTAGTTT ACGGTCAGCT GCCTATAATC TTCTGTGTGC CTTAACTTGT
    ACCTTTAATT TAAAAATCGA GGGCCAGTTG CTAGAGACGT CAGGTTTATG TATCCCTGCC
    AACAACACCC TCTTTATTGT CTCTATTAGT AAGACACTGG CAGCCAATGA GCCACACCTC
    ACCTTAGAAT TTTTGGAAGA GTGTATTTCT GGATTTAGCA AATCTAGTAT TGAACTGAAA
    CACCTTTGTT TGGAATACAT GACTCCATGG CTGTCAAATC TAGTCCGTTT TTGTAAGCAC
    AATGATGATG CCAAAAGACA AAGAGTTACT GCTATTCTTG ATAAGCTGAT AACAATGACC
    ATAAATGAGA AACAGATGTA CCCATCTATT CAAGCAAAAA TATGGGGGAG CCTTGGGCAG
    ATTACAGATC TCCTTGATGT TGTGCTAGAC AGTTTCATCA AAACCAGTGC AACAGGTGGC
    TTGGGATCAA TAAAAGCCGA GGTGATGGCA GATACTGCTG TAGCTCTGGC GTCTGGAAAT
    GTGAAATTGG TTTCTAGCAA GGTTATTGGA AGGATGTGCA AAATAATTGA CAAGACATGC
    TTATCTCCAA CTCCTACTTT AGAACAGCAT CTTATGTGGG ATGACATTGC GATTCTAGCG
    CGCTACATGC TGATGTTGTC CTTCAACAAC TCCCTCGACG TGGCGGCTCA TCTCCCCTAC
    CTCTTCCACG TTGTGACTTT CTTAGTAGCC ACAGGGCCAC TCTCCCTTAG AGCTTCCACA
    CATGGATTGG TCATTAATAT CATTCACTCT CTGTGCACTT GTTCACAGCT TCATTTTAGT
    GAGGAGACCA AGCAAGTTTT GAGACTCAGT TTGACAGAGT TCTCATTACC CAAATTTTAC
    TTACTGTTTG GCATTAGCAA AGTCAAGTCA GCTGCTGTGA TTGCCTTCCG CTCCAGTTAC
    CGGGACAGGT CGTTCTCTCC TGGCTCCTAC GAGAGGGAGA CTTTTGCTTT GACATCCTTG
    GAAACAGTCA CGGAAGCTTT GTTGGAGATC ATGGAGGCAT GTATGAGAGA TATTCCAACA
    TGCAAGTGGC TGGATCAGTG GACAGAACTA GCTCAAAGGT TTGCATTCCA GTACAACCCG
    TCCCTGCAAC CGAGAGCTCT GGTCGTCTTT GGCTGCATTA GTAAGCGAGT GTCTCACGGG
    CAGATAAAGC AGATTATCCG CATTCTTAGC AAGGCGCTTG AGAGTTGCTT AAAAGGACCT
    GATACTTACA ACAGTCAAGT TCTGATAGAA GCTACAGTAA TAGCACTAAC CAAATTACAA
    CCACTTCTTA ATAAGGACTC CCCTCTGCAC AAAGCCCTCT TTTGGGTAGC TGTGGCCGTG
    CTGCAGCTCG ATGAGGTCAA CTTGTATTCA GCAGGCACCG CACTTCTTGA GCAAAACCTG
    CACACCTTAG ACAGTCTCCG GATATTCAAT GACAAGAGTC CAGAGGAAGT ATTTATGGCA
    ATTCGGAATC CTCTGGAATG GCACTGCAAG CAAATGGATC ATTTTGTTGG ACTCAATTTC
    AACTCTAACT TTAACTTTGC ACTGGTTGGA CACCTCTTAA AAGGGTACAG ACATCCTTCA
    CCTGCTATTG TTGCACGAAC AGTCAGAATT TTGCATACAC TACTAACTCT GGTTAACAAA
    CATAGAAATT GTGACAAATT TGAGGTGAAT ACGCAGAGCG TGGCTTACTT AGCAGCTTTA
    CTCACAGTAT CTGAGGAGGT TCGAAGTCGC TGTAGCCTAA AACATAGAAA GTCTCTTCTT
    CTTACTGATA TTTCAATGGA AAATATTCCT ATGGATACAT ATCCCATTCA TCATGGCGAC
    CCCAGCTATA GGACACTAAA GGAGAGTCAG CCATGGTCCT CTCCCAAAGG CTCGGAAGGG
    TACCTTGCAG CCACCTATCC AACCGTGGGC CAGACCAGCC CCCGAGCCCG GAAATCCATG
    AGCTTGGACA TGGGGCAGCC TTCTCAGGCC AACACTAAGA AGTTGCTGGG AACAAGGAAA
    AGTTTTGATC ACTTGATATC AGACACGAAG GCTCCTAAAA GGCAAGAAGT GGAATCAGGG
    ATCACAACAC CCCCCAAAAT GAGGAGAGTA GCAGAAACTG ATTATGAAAT GGAAACTCAA
    AGGATTTCCT CATCACAACA GCACCCACAT TTACGTAAAG TTTCAGTGTC TGAATCAAAT
    GTTCTCTTGG ATGAAGAAGT ACTTACTGAC CCGAAGATCC AGGCATTGCT GCTTACTGTT
    CTGGCTACAC TGGTGAAATA CACCACGGAT GAGTTTGATC AACGAATTCT TTATGAATAC
    TTAGCAGAAG CCAGTGTTGT TTTCCCCAAA GTTTTTCCTG TTGTGCACAA TTTGTTGGAC
    TCCAAGATCA ACACCCTGTT GTCATTGTGC CAAGATCCAA ATTTGTTAAA TCCGATTCAT
    GGGATTGTGC AGAGTGTGGT ATACTACGAG GAGTCCCCAC CACAGTACCA GACGTCTTAC
    CTGCAGAGTT TTGGTTTTAA TGGCTTGTGG CGGTTTGCAG GACCGTTTTC AAAGCAAACA
    CAAATCCCAG ACTATGCTGA GCTTATTGTT AAGTTTCTTG ATGCCTTGAT TGACACGTAT
    TTGCCTGGAA TTGATGAAGA AACCAGTGAG GAGTCCCTCC TGACACCCAC ATCTCCTTAT
    CCTCCTGCAG TGCAGAGCCA GCTTAGTATT ACTGCTAACC TTAACCTTTC CAATTCCATG
    ACCTCACTTG CAACTTCCCA GCATTCCCCA GGAATCGACA AGGAGAACGT TGAACTCTCC
    CCAACCACTG GGCACTGTAA CAGTGGACGA ACTCGCCATG GATCCGCAAG CCAAGTGCAG
    AAGCAAAGAA GCGCTGGCAG TTTCAAACGT AATAGCATTA AGAAGATCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508830.1
    CDS165..8558
    Translation

    Target ORF information:

    RefSeq Version XM_023653062.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGGATAG TGCGGCAGGG TGCAGTGGAA CGCCGCCGAT ATGCCGGCAA
    GCCCAGACCA AACTGGAAGT GGCCCTGTAC ATGTTTCTGT GGAGCCCTGA CACCGAAGCT
    GTTCTGGTTG CCATGTCCTG TTTCCGCCAC CTCTGTGAGG AAGCGGATAT CCGGTGTGGG
    GTGGACGAAG TATCGGTGCA CAACTTCTTG CCGAACTATA ACACATTCAT GGAATTTGCG
    TCTGTCAGCA ATATGATGTC AACAGGAAGA GCAGCACTTC AGAAAAGAGT GATGGCACTA
    CTGAGGCGCA TTGAACATCC CACTGCAGGA AACACTGAGG CTTGGGAAGA TACACATGCA
    AAGTGGGAAC AAGCTACAAA ACTAATCCTC AACTATCCAA AAGCCAAAAT GGAAGATGGC
    CAGGCTGCTG AAAGCCTTCA TAAGACCATT GTTAAGAGGC GAATGTCTCA TGTGAGTGGA
    GGAGGATCCA TAGATTTGTC TGACACGGAC TCCCTTCAGG AATGGATCAA CATGACCGGC
    TTCCTCTGTG CCCTTGGGGG AGTGTGCCTG CAGCAGAGAA GCAATTCTGG CCTGGCAACC
    TATAGCCCAC CCATGGGCCC AGTCAGTGAA CGCAAGGGTT CCATGATTTC AGTGATGTCC
    TCAGAGGGAA ATGCAGACAC ACCTGTCAGC AAATTTATGG ATCGGCTGCT ATCCTTAATG
    GTGTGTAACC ATGAGAAAGT GGGACTTCAA ATACGCACCA ACGTTAAGGA TCTGGTGGGT
    TTAGAATTGA GTCCTGCTCT TTATCCAATG CTGTTTAACA AATTGAAGAA CACCATCAGC
    AAGTTTTTTG ACTCCCAAGG ACAGGTTTTA TTGACTGACA CCAATACTCA ATTTGTAGAG
    CAAACCATAG CTATAATGAA GAACTTACTA GATAATCATA CTGAAGGCAG CTCCGAACAT
    CTGGGGCAAG CTAGCATTGA AACGATGATG TTAAATCTGG TCAGATATGT TCGTGTACTT
    GGGAACATGG TCCATGCAAT TCAAATAAAA ACGAAACTGT GTCAGCTAGT CGAGGTAATG
    ATGGCAAGAA GAGATGACCT CTCATTTTGT CAAGAGATGA AATTTAGGAA TAAGATGGTA
    GAATATCTGA CAGATTGGGT TATGGGAACA TCAAACCAAG CAGCAGATGA TGATGTAAAA
    TGTCTTACAA GAGATCTGGA CCAGGCAAGC ATGGAAGCAG TAGTTTCACT CCTGGCTGGT
    CTCCCGCTGC AGCCTGAGGA AGGAGATGGT GTGGAACTCA TGGAAGCCAA GTCACAGCTA
    TTTCTTAAGT ATTTCACATT GTTTATGAAC CTTTTGAATG ACTGTAGTGA AGTTGAAGAT
    GAAAATGCAC AAACAGGTGG CAGGAAGCGT GGCATGTCTC GGAGGCTGGC CTCACTGAGG
    CACTGCACAG TCCTTGCAAT GTCAAACTTA CTTAACGCTA ACGTGGACAG TGGCCTCATG
    CACTCCATAG GTTTAGGTTA TCACAAAGAT CTCCAGACAA GAGCTACATT TATGGAAGTT
    CTGACAAAAA TCCTCCAACA AGGCACAGAA TTTGACACAC TTGCAGAAAC GGTGTTGGCA
    GATCGGTTTG AGAGGCTGGT GGAGTTGGTC ACAATGATGG GTGATCAGGG AGAGCTCCCT
    ATAGCCATGG CTCTGGCCAA TGTGGTCCCT TGTTCTCAGT GGGATGAACT AGCTCGAGTT
    CTAGTGACTC TCTTTGATTC TCGGCATTTA CTCTACCAAC TGCTCTGGAA CATGTTTTCT
    AAGGAAGTAG AATTGGCAGA CTCCATGCAG ACTCTTTTCC GAGGCAACAG CTTAGCCAGT
    AAAATAATGA CCTTCTGTTT CAAGGTATAT GGTGCTACCT ACCTACAAAA ACTCCTGGAT
    CCTTTGTTAC GAATTGTGAT CACATCCTCT GATTGGCAAC ATGTTAGCTT TGAAGTGGAT
    CCTACCAGGT TAGAACAGTC AGAGAGCCTT GAGGAGAACC AGCGGAGCCT CCTTCAGATG
    ACTGAAAAGT TCTTCCACGC CATCATCAGT TCCTCCTCGG AATTCCCCCC TCAGCTTCGA
    AGTGTGTGCC ACTGTCTATA CCAGGCAACT TGCCACTCCC TACTGAATAC AGCTACAGTA
    AAAGAAAAAA AGGAAAACAA AAAATCAGTG GTTAGCCAGC GTTTCCCTCA GAACAGCATC
    GGTGCAGTGG GAAGTGCCAT GTTCCTCAGA TTCATCAATC CTGCCATCGT CTCACCGTAT
    GAAGCAGGCA TTTTAGATAA AAAGCCACCA CCTAGAATCG AAAGGGGCTT GAAGTTAATG
    TCAAAGATAC TTCAGAGTAT TGCCAATCAT GTTCTCTTCA CAAAAGAAGA GCATATGCGG
    CCTTTTAATG ATTTTGTGAA AAGCAACTTT GATGCAGCAC GAAGGTTTTT CCTTGATATA
    GCATCCGATT GTCCTACAAG TGATGCAGTG AATCACAGTC TTTCCTTCAT CAGCGATGGC
    AACGTGCTGG CTCTACATCG TCTCCTCTGG AACAATCAAG AGAAGATTGG CCAGTATCTT
    TCCAGCAATA GGGATCATAA AGCTGTTGGA AGACGACCTT TTGATAAGAT GGCAACACTT
    CTTGCATACC TGGGTCCTCC AGAGCACAAA CCTGTGGCAG ATACACACTG GTCCAGCCTT
    AACCTTACCA GTTCAAAGTT CGAGGAATTT ATGACTAGGC ATCAAGTACA TGAAAAAGAA
    GAGTTCAAGG CCTTGAAAAC ATTAAGTATT TTCTACCAAG CTGGGACTTC CAGAGCTGGG
    AATCCTATAT TTTATTATGT TGCACGGAGG TTCAAAACTG GTCAAATCAA TGGCGATTTG
    CTGATCTACC ATGTCTTGCT GACTCTAAAG CCATATTATG CAAAGCCATA TGAAATTGTA
    GTGGACCTTA CCCATACCGG GCCTAGCAAT CGCTTTAAAA CGGACTTTCT CTCCAAGTGG
    TTTGTTGTTT TTCCTGGCTT TGCTTATGAC AATGTCTCTG CAGTCTATAT CTATAACTGT
    AACTCCTGGG TCAGGGAGTA CACCAAGTAT CACGAGCGGC TGCTCACTGG CCTCAAAGGC
    AGCAAAAGGC TCATTTTCAT AGACTGTCCT GGGAAGCTGG CTGAGCACGT AGAGCACGAA
    CAACAGAAAC TACCTGCTGC CACTTTGGCT TTAGAAGAGG ACCTGAAAGT GTTCCACAAT
    GCTCTCAAGC TGGCTCACAA AGACACCAAG GTTTCCATTA AAGTTGGTTC TACTGCTGTT
    CAAGTGACCT CAGCAGAGCG AACGAAAGTC CTAGGGCAAT CAGTCTTTCT AAACGATATC
    TACTATGCTT CGGAAATTGA AGAGATCTGC CTGGTGGATG AGAACCAGTT CACCTTAACC
    ATTGCAAACC AGGGCACACC GCTCACCTTC ATGCACCAGG AGTGTGAAGC CATCGTCCAG
    TCCATCATTC ACATCCGGAC ACGCTGGGAG CTGTCACAGC CCGACTCCAT CCCCCAGCAC
    ACCAAGATCC GGCCAAAAGA TGTCCCTGGG ACACTGCTCA ATATCGCGTT ACTTAATTTA
    GGCAGTTCAG ACCCTAGTTT ACGGTCAGCT GCCTATAATC TTCTGTGTGC CTTAACTTGT
    ACCTTTAATT TAAAAATCGA GGGCCAGTTG CTAGAGACGT CAGGTTTATG TATCCCTGCC
    AACAACACCC TCTTTATTGT CTCTATTAGT AAGACACTGG CAGCCAATGA GCCACACCTC
    ACCTTAGAAT TTTTGGAAGA GTGTATTTCT GGATTTAGCA AATCTAGTAT TGAACTGAAA
    CACCTTTGTT TGGAATACAT GACTCCATGG CTGTCAAATC TAGTCCGTTT TTGTAAGCAC
    AATGATGATG CCAAAAGACA AAGAGTTACT GCTATTCTTG ATAAGCTGAT AACAATGACC
    ATAAATGAGA AACAGATGTA CCCATCTATT CAAGCAAAAA TATGGGGGAG CCTTGGGCAG
    ATTACAGATC TCCTTGATGT TGTGCTAGAC AGTTTCATCA AAACCAGTGC AACAGGTGGC
    TTGGGATCAA TAAAAGCCGA GGTGATGGCA GATACTGCTG TAGCTCTGGC GTCTGGAAAT
    GTGAAATTGG TTTCTAGCAA GGTTATTGGA AGGATGTGCA AAATAATTGA CAAGACATGC
    TTATCTCCAA CTCCTACTTT AGAACAGCAT CTTATGTGGG ATGACATTGC GATTCTAGCG
    CGCTACATGC TGATGTTGTC CTTCAACAAC TCCCTCGACG TGGCGGCTCA TCTCCCCTAC
    CTCTTCCACG TTGTGACTTT CTTAGTAGCC ACAGGGCCAC TCTCCCTTAG AGCTTCCACA
    CATGGATTGG TCATTAATAT CATTCACTCT CTGTGCACTT GTTCACAGCT TCATTTTAGT
    GAGGAGACCA AGCAAGTTTT GAGACTCAGT TTGACAGAGT TCTCATTACC CAAATTTTAC
    TTACTGTTTG GCATTAGCAA AGTCAAGTCA GCTGCTGTGA TTGCCTTCCG CTCCAGTTAC
    CGGGACAGGT CGTTCTCTCC TGGCTCCTAC GAGAGGGAGA CTTTTGCTTT GACATCCTTG
    GAAACAGTCA CGGAAGCTTT GTTGGAGATC ATGGAGGCAT GTATGAGAGA TATTCCAACA
    TGCAAGTGGC TGGATCAGTG GACAGAACTA GCTCAAAGGT TTGCATTCCA GTACAACCCG
    TCCCTGCAAC CGAGAGCTCT GGTCGTCTTT GGCTGCATTA GTAAGCGAGT GTCTCACGGG
    CAGATAAAGC AGATTATCCG CATTCTTAGC AAGGCGCTTG AGAGTTGCTT AAAAGGACCT
    GATACTTACA ACAGTCAAGT TCTGATAGAA GCTACAGTAA TAGCACTAAC CAAATTACAA
    CCACTTCTTA ATAAGGACTC CCCTCTGCAC AAAGCCCTCT TTTGGGTAGC TGTGGCCGTG
    CTGCAGCTCG ATGAGGTCAA CTTGTATTCA GCAGGCACCG CACTTCTTGA GCAAAACCTG
    CACACCTTAG ACAGTCTCCG GATATTCAAT GACAAGAGTC CAGAGGAAGT ATTTATGGCA
    ATTCGGAATC CTCTGGAATG GCACTGCAAG CAAATGGATC ATTTTGTTGG ACTCAATTTC
    AACTCTAACT TTAACTTTGC ACTGGTTGGA CACCTCTTAA AAGGGTACAG ACATCCTTCA
    CCTGCTATTG TTGCACGAAC AGTCAGAATT TTGCATACAC TACTAACTCT GGTTAACAAA
    CATAGAAATT GTGACAAATT TGAGGTGAAT ACGCAGAGCG TGGCTTACTT AGCAGCTTTA
    CTCACAGTAT CTGAGGAGGT TCGAAGTCGC TGTAGCCTAA AACATAGAAA GTCTCTTCTT
    CTTACTGATA TTTCAATGGA AAATATTCCT ATGGATACAT ATCCCATTCA TCATGGCGAC
    CCCAGCTATA GGACACTAAA GGAGAGTCAG CCATGGTCCT CTCCCAAAGG CTCGGAAGGG
    TACCTTGCAG CCACCTATCC AACCGTGGGC CAGACCAGCC CCCGAGCCCG GAAATCCATG
    AGCTTGGACA TGGGGCAGCC TTCTCAGGCC AACACTAAGA AGTTGCTGGG AACAAGGAAA
    AGTTTTGATC ACTTGATATC AGACACGAAG GCTCCTAAAA GGCAAGAAGT GGAATCAGGG
    ATCACAACAC CCCCCAAAAT GAGGAGAGTA GCAGAAACTG ATTATGAAAT GGAAACTCAA
    AGGATTTCCT CATCACAACA GCACCCACAT TTACGTAAAG TTTCAGTGTC TGAATCAAAT
    GTTCTCTTGG ATGAAGAAGT ACTTACTGAC CCGAAGATCC AGGCATTGCT GCTTACTGTT
    CTGGCTACAC TGGTGAAATA CACCACGGAT GAGTTTGATC AACGAATTCT TTATGAATAC
    TTAGCAGAAG CCAGTGTTGT TTTCCCCAAA GTTTTTCCTG TTGTGCACAA TTTGTTGGAC
    TCCAAGATCA ACACCCTGTT GTCATTGTGC CAAGATCCAA ATTTGTTAAA TCCGATTCAT
    GGGATTGTGC AGAGTGTGGT ATACTACGAG GAGTCCCCAC CACAGTACCA GACGTCTTAC
    CTGCAGAGTT TTGGTTTTAA TGGCTTGTGG CGGTTTGCAG GACCGTTTTC AAAGCAAACA
    CAAATCCCAG ACTATGCTGA GCTTATTGTT AAGTTTCTTG ATGCCTTGAT TGACACGTAT
    TTGCCTGGAA TTGATGAAGA AACCAGTGAG GAGTCCCTCC TGACACCCAC ATCTCCTTAT
    CCTCCTGCAG TGCAGAGCCA GCTTAGTATT ACTGCTAACC TTAACCTTTC CAATTCCATG
    ACCTCACTTG CAACTTCCCA GCATTCCCCA GGAATCGACA AGGAGAACGT TGAACTCTCC
    CCAACCACTG GGCACTGTAA CAGTGGACGA ACTCGCCATG GATCCGCAAG CCAAGTGCAG
    AAGCAAAGAA GCGCTGGCAG TTTCAAACGT AATAGCATTA AGAAGATCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45491
    Clone ID Related Accession (Same CDS sequence) XM_023653063.1
    Accession Version XM_023653063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8373bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGCAA CTTGCCACTC CCTACTGAAT ACAGCTACAG TAAAAGAAAA AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGTTTCCCT CAGAACAGCA TCGGTGCAGT GGGAAGTGCC
    ATGTTCCTCA GATTCATCAA TCCTGCCATC GTCTCACCGT ATGAAGCAGG CATTTTAGAT
    AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT
    ATTGCCAATC ATGTTCTCTT CACAAAAGAA GAGCATATGC GGCCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGATGCAGC ACGAAGGTTT TTCCTTGATA TAGCATCCGA TTGTCCTACA
    AGTGATGCAG TGAATCACAG TCTTTCCTTC ATCAGCGATG GCAACGTGCT GGCTCTACAT
    CGTCTCCTCT GGAACAATCA AGAGAAGATT GGCCAGTATC TTTCCAGCAA TAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCAACAC TTCTTGCATA CCTGGGTCCT
    CCAGAGCACA AACCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTCGAGGAAT TTATGACTAG GCATCAAGTA CATGAAAAAG AAGAGTTCAA GGCCTTGAAA
    ACATTAAGTA TTTTCTACCA AGCTGGGACT TCCAGAGCTG GGAATCCTAT ATTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGTCAAATC AATGGCGATT TGCTGATCTA CCATGTCTTG
    CTGACTCTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACC
    GGGCCTAGCA ATCGCTTTAA AACGGACTTT CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC
    TTTGCTTATG ACAATGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TACACCAAGT ATCACGAGCG GCTGCTCACT GGCCTCAAAG GCAGCAAAAG GCTCATTTTC
    ATAGACTGTC CTGGGAAGCT GGCTGAGCAC GTAGAGCACG AACAACAGAA ACTACCTGCT
    GCCACTTTGG CTTTAGAAGA GGACCTGAAA GTGTTCCACA ATGCTCTCAA GCTGGCTCAC
    AAAGACACCA AGGTTTCCAT TAAAGTTGGT TCTACTGCTG TTCAAGTGAC CTCAGCAGAG
    CGAACGAAAG TCCTAGGGCA ATCAGTCTTT CTAAACGATA TCTACTATGC TTCGGAAATT
    GAAGAGATCT GCCTGGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAGGGCACA
    CCGCTCACCT TCATGCACCA GGAGTGTGAA GCCATCGTCC AGTCCATCAT TCACATCCGG
    ACACGCTGGG AGCTGTCACA GCCCGACTCC ATCCCCCAGC ACACCAAGAT CCGGCCAAAA
    GATGTCCCTG GGACACTGCT CAATATCGCG TTACTTAATT TAGGCAGTTC AGACCCTAGT
    TTACGGTCAG CTGCCTATAA TCTTCTGTGT GCCTTAACTT GTACCTTTAA TTTAAAAATC
    GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA TGTATCCCTG CCAACAACAC CCTCTTTATT
    GTCTCTATTA GTAAGACACT GGCAGCCAAT GAGCCACACC TCACCTTAGA ATTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAACTGA AACACCTTTG TTTGGAATAC
    ATGACTCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ACAATGATGA TGCCAAAAGA
    CAAAGAGTTA CTGCTATTCT TGATAAGCTG ATAACAATGA CCATAAATGA GAAACAGATG
    TACCCATCTA TTCAAGCAAA AATATGGGGG AGCCTTGGGC AGATTACAGA TCTCCTTGAT
    GTTGTGCTAG ACAGTTTCAT CAAAACCAGT GCAACAGGTG GCTTGGGATC AATAAAAGCC
    GAGGTGATGG CAGATACTGC TGTAGCTCTG GCGTCTGGAA ATGTGAAATT GGTTTCTAGC
    AAGGTTATTG GAAGGATGTG CAAAATAATT GACAAGACAT GCTTATCTCC AACTCCTACT
    TTAGAACAGC ATCTTATGTG GGATGACATT GCGATTCTAG CGCGCTACAT GCTGATGTTG
    TCCTTCAACA ACTCCCTCGA CGTGGCGGCT CATCTCCCCT ACCTCTTCCA CGTTGTGACT
    TTCTTAGTAG CCACAGGGCC ACTCTCCCTT AGAGCTTCCA CACATGGATT GGTCATTAAT
    ATCATTCACT CTCTGTGCAC TTGTTCACAG CTTCATTTTA GTGAGGAGAC CAAGCAAGTT
    TTGAGACTCA GTTTGACAGA GTTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CAGCTGCTGT GATTGCCTTC CGCTCCAGTT ACCGGGACAG GTCGTTCTCT
    CCTGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCT TGGAAACAGT CACGGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG GTTTGCATTC CAGTACAACC CGTCCCTGCA ACCGAGAGCT
    CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA GTGTCTCACG GGCAGATAAA GCAGATTATC
    CGCATTCTTA GCAAGGCGCT TGAGAGTTGC TTAAAAGGAC CTGATACTTA CAACAGTCAA
    GTTCTGATAG AAGCTACAGT AATAGCACTA ACCAAATTAC AACCACTTCT TAATAAGGAC
    TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA GCTGTGGCCG TGCTGCAGCT CGATGAGGTC
    AACTTGTATT CAGCAGGCAC CGCACTTCTT GAGCAAAACC TGCACACCTT AGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCAGAGGAA GTATTTATGG CAATTCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAAATGGA TCATTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT
    GCACTGGTTG GACACCTCTT AAAAGGGTAC AGACATCCTT CACCTGCTAT TGTTGCACGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTGGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACGCAGAG CGTGGCTTAC TTAGCAGCTT TACTCACAGT ATCTGAGGAG
    GTTCGAAGTC GCTGTAGCCT AAAACATAGA AAGTCTCTTC TTCTTACTGA TATTTCAATG
    GAAAATATTC CTATGGATAC ATATCCCATT CATCATGGCG ACCCCAGCTA TAGGACACTA
    AAGGAGAGTC AGCCATGGTC CTCTCCCAAA GGCTCGGAAG GGTACCTTGC AGCCACCTAT
    CCAACCGTGG GCCAGACCAG CCCCCGAGCC CGGAAATCCA TGAGCTTGGA CATGGGGCAG
    CCTTCTCAGG CCAACACTAA GAAGTTGCTG GGAACAAGGA AAAGTTTTGA TCACTTGATA
    TCAGACACGA AGGCTCCTAA AAGGCAAGAA GTGGAATCAG GGATCACAAC ACCCCCCAAA
    ATGAGGAGAG TAGCAGAAAC TGATTATGAA ATGGAAACTC AAAGGATTTC CTCATCACAA
    CAGCACCCAC ATTTACGTAA AGTTTCAGTG TCTGAATCAA ATGTTCTCTT GGATGAAGAA
    GTACTTACTG ACCCGAAGAT CCAGGCATTG CTGCTTACTG TTCTGGCTAC ACTGGTGAAA
    TACACCACGG ATGAGTTTGA TCAACGAATT CTTTATGAAT ACTTAGCAGA AGCCAGTGTT
    GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC AATTTGTTGG ACTCCAAGAT CAACACCCTG
    TTGTCATTGT GCCAAGATCC AAATTTGTTA AATCCGATTC ATGGGATTGT GCAGAGTGTG
    GTATACTACG AGGAGTCCCC ACCACAGTAC CAGACGTCTT ACCTGCAGAG TTTTGGTTTT
    AATGGCTTGT GGCGGTTTGC AGGACCGTTT TCAAAGGAAT CGACAAGGAG AACGTTGAAC
    TCTCCCCAAC CACTGGGCAC TGTAACAGTG GACGAACTCG CCATGGATCC GCAAGCCAAG
    TGCAGAAGCA AAGAAGCGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG ATCGTGTGAA
    GCTTTCTTTC TTTCCTTTTT CAAACAGACG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508831.1
    CDS166..8538
    Translation

    Target ORF information:

    RefSeq Version XM_023653063.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACGGA GCATAACAAG
    GAATGTCTTA TCAATATTTC CAAGTACAAG TTTTCTCTGG TTATAAGCGG CCTCACCACT
    ATCTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATAATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCGAAGGAC
    ACAATGAGAT TAGATGAAAC AATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACGCCT GTCGTGAAGG AAACCAACAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGTTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TGACTGTTTG TTCAGAAGAT AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCACAGTTA GCAGTTATAA ATAGCCTGGA AAAAGCATTT
    TGGAACTGGG TCGAAAATTA TCCAGATGAA TTTACAAAGC TATACCAGAT TCCACAGACT
    GATATGGCTG AGTGTGCGGA AAAACTGTTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCGCTTCAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAGGA TGTGGTTGAT GAAAGCAACA TGAATAAGAA GTTGTTTCTG
    GACAGTCTGC GGAAAGCGCT TGCTGGCCAT GGAGGAAGCA GGCAGCTGAC TGAAAGTGCC
    GCCACTGCCT GTGTGAAGCT GTGCAAAGCG AGTACTTACA TCAACTGGGA AGACAACTCG
    GTCATTTTCC TACTAGTTCA GTCCATGGTG GTCGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAACCAT TCTCAAGAGG CAGTCAACCT GCAGACGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCTCCTT CTACGTTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCTGCACTGG ATTGGTGGCC TAAGATCGAT
    GCCGTATATT GTCACTCAGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCG
    GTGCAGGGTT GTGGAGCACA CCCGGCGATA CGCATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTTT TGTCTATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCCAGAAAAC AGGGGCCTGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAGCTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTGCTTCATC AGTTAGACAG CATCGATTTG TGGAATCCTG ATGCTCCTGT GGAAACATTT
    TGGGAAATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AGTTAACTAG CCAGCAAATG
    CTTAGTAGCA CAGAAATTCT CAAATGGTTG CGGGAAATTT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGGAGT TCCTGTCACT TTCTCTTCCT TTATGGGGTA
    GGATGTGATG TTCCTTCCAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAGTTA
    TTACGTACTT ATACTCCTGG AGCCTCTCTC CGGAAGGGAA AAGGAAACTC ATCCATGGAT
    AGTGCGGCAG GGTGCAGTGG AACGCCGCCG ATATGCCGGC AAGCCCAGAC CAAACTGGAA
    GTGGCCCTGT ACATGTTTCT GTGGAGCCCT GACACCGAAG CTGTTCTGGT TGCCATGTCC
    TGTTTCCGCC ACCTCTGTGA GGAAGCGGAT ATCCGGTGTG GGGTGGACGA AGTATCGGTG
    CACAACTTCT TGCCGAACTA TAACACATTC ATGGAATTTG CGTCTGTCAG CAATATGATG
    TCAACAGGAA GAGCAGCACT TCAGAAAAGA GTGATGGCAC TACTGAGGCG CATTGAACAT
    CCCACTGCAG GAAACACTGA GGCTTGGGAA GATACACATG CAAAGTGGGA ACAAGCTACA
    AAACTAATCC TCAACTATCC AAAAGCCAAA ATGGAAGATG GCCAGGCTGC TGAAAGCCTT
    CATAAGACCA TTGTTAAGAG GCGAATGTCT CATGTGAGTG GAGGAGGATC CATAGATTTG
    TCTGACACGG ACTCCCTTCA GGAATGGATC AACATGACCG GCTTCCTCTG TGCCCTTGGG
    GGAGTGTGCC TGCAGCAGAG AAGCAATTCT GGCCTGGCAA CCTATAGCCC ACCCATGGGC
    CCAGTCAGTG AACGCAAGGG TTCCATGATT TCAGTGATGT CCTCAGAGGG AAATGCAGAC
    ACACCTGTCA GCAAATTTAT GGATCGGCTG CTATCCTTAA TGGTGTGTAA CCATGAGAAA
    GTGGGACTTC AAATACGCAC CAACGTTAAG GATCTGGTGG GTTTAGAATT GAGTCCTGCT
    CTTTATCCAA TGCTGTTTAA CAAATTGAAG AACACCATCA GCAAGTTTTT TGACTCCCAA
    GGACAGGTTT TATTGACTGA CACCAATACT CAATTTGTAG AGCAAACCAT AGCTATAATG
    AAGAACTTAC TAGATAATCA TACTGAAGGC AGCTCCGAAC ATCTGGGGCA AGCTAGCATT
    GAAACGATGA TGTTAAATCT GGTCAGATAT GTTCGTGTAC TTGGGAACAT GGTCCATGCA
    ATTCAAATAA AAACGAAACT GTGTCAGCTA GTCGAGGTAA TGATGGCAAG AAGAGATGAC
    CTCTCATTTT GTCAAGAGAT GAAATTTAGG AATAAGATGG TAGAATATCT GACAGATTGG
    GTTATGGGAA CATCAAACCA AGCAGCAGAT GATGATGTAA AATGTCTTAC AAGAGATCTG
    GACCAGGCAA GCATGGAAGC AGTAGTTTCA CTCCTGGCTG GTCTCCCGCT GCAGCCTGAG
    GAAGGAGATG GTGTGGAACT CATGGAAGCC AAGTCACAGC TATTTCTTAA GTATTTCACA
    TTGTTTATGA ACCTTTTGAA TGACTGTAGT GAAGTTGAAG ATGAAAATGC ACAAACAGGT
    GGCAGGAAGC GTGGCATGTC TCGGAGGCTG GCCTCACTGA GGCACTGCAC AGTCCTTGCA
    ATGTCAAACT TACTTAACGC TAACGTGGAC AGTGGCCTCA TGCACTCCAT AGGTTTAGGT
    TATCACAAAG ATCTCCAGAC AAGAGCTACA TTTATGGAAG TTCTGACAAA AATCCTCCAA
    CAAGGCACAG AATTTGACAC ACTTGCAGAA ACGGTGTTGG CAGATCGGTT TGAGAGGCTG
    GTGGAGTTGG TCACAATGAT GGGTGATCAG GGAGAGCTCC CTATAGCCAT GGCTCTGGCC
    AATGTGGTCC CTTGTTCTCA GTGGGATGAA CTAGCTCGAG TTCTAGTGAC TCTCTTTGAT
    TCTCGGCATT TACTCTACCA ACTGCTCTGG AACATGTTTT CTAAGGAAGT AGAATTGGCA
    GACTCCATGC AGACTCTTTT CCGAGGCAAC AGCTTAGCCA GTAAAATAAT GACCTTCTGT
    TTCAAGGTAT ATGGTGCTAC CTACCTACAA AAACTCCTGG ATCCTTTGTT ACGAATTGTG
    ATCACATCCT CTGATTGGCA ACATGTTAGC TTTGAAGTGG ATCCTACCAG GTTAGAACAG
    TCAGAGAGCC TTGAGGAGAA CCAGCGGAGC CTCCTTCAGA TGACTGAAAA GTTCTTCCAC
    GCCATCATCA GTTCCTCCTC GGAATTCCCC CCTCAGCTTC GAAGTGTGTG CCACTGTCTA
    TACCAGGCAA CTTGCCACTC CCTACTGAAT ACAGCTACAG TAAAAGAAAA AAAGGAAAAC
    AAAAAATCAG TGGTTAGCCA GCGTTTCCCT CAGAACAGCA TCGGTGCAGT GGGAAGTGCC
    ATGTTCCTCA GATTCATCAA TCCTGCCATC GTCTCACCGT ATGAAGCAGG CATTTTAGAT
    AAAAAGCCAC CACCTAGAAT CGAAAGGGGC TTGAAGTTAA TGTCAAAGAT ACTTCAGAGT
    ATTGCCAATC ATGTTCTCTT CACAAAAGAA GAGCATATGC GGCCTTTTAA TGATTTTGTG
    AAAAGCAACT TTGATGCAGC ACGAAGGTTT TTCCTTGATA TAGCATCCGA TTGTCCTACA
    AGTGATGCAG TGAATCACAG TCTTTCCTTC ATCAGCGATG GCAACGTGCT GGCTCTACAT
    CGTCTCCTCT GGAACAATCA AGAGAAGATT GGCCAGTATC TTTCCAGCAA TAGGGATCAT
    AAAGCTGTTG GAAGACGACC TTTTGATAAG ATGGCAACAC TTCTTGCATA CCTGGGTCCT
    CCAGAGCACA AACCTGTGGC AGATACACAC TGGTCCAGCC TTAACCTTAC CAGTTCAAAG
    TTCGAGGAAT TTATGACTAG GCATCAAGTA CATGAAAAAG AAGAGTTCAA GGCCTTGAAA
    ACATTAAGTA TTTTCTACCA AGCTGGGACT TCCAGAGCTG GGAATCCTAT ATTTTATTAT
    GTTGCACGGA GGTTCAAAAC TGGTCAAATC AATGGCGATT TGCTGATCTA CCATGTCTTG
    CTGACTCTAA AGCCATATTA TGCAAAGCCA TATGAAATTG TAGTGGACCT TACCCATACC
    GGGCCTAGCA ATCGCTTTAA AACGGACTTT CTCTCCAAGT GGTTTGTTGT TTTTCCTGGC
    TTTGCTTATG ACAATGTCTC TGCAGTCTAT ATCTATAACT GTAACTCCTG GGTCAGGGAG
    TACACCAAGT ATCACGAGCG GCTGCTCACT GGCCTCAAAG GCAGCAAAAG GCTCATTTTC
    ATAGACTGTC CTGGGAAGCT GGCTGAGCAC GTAGAGCACG AACAACAGAA ACTACCTGCT
    GCCACTTTGG CTTTAGAAGA GGACCTGAAA GTGTTCCACA ATGCTCTCAA GCTGGCTCAC
    AAAGACACCA AGGTTTCCAT TAAAGTTGGT TCTACTGCTG TTCAAGTGAC CTCAGCAGAG
    CGAACGAAAG TCCTAGGGCA ATCAGTCTTT CTAAACGATA TCTACTATGC TTCGGAAATT
    GAAGAGATCT GCCTGGTGGA TGAGAACCAG TTCACCTTAA CCATTGCAAA CCAGGGCACA
    CCGCTCACCT TCATGCACCA GGAGTGTGAA GCCATCGTCC AGTCCATCAT TCACATCCGG
    ACACGCTGGG AGCTGTCACA GCCCGACTCC ATCCCCCAGC ACACCAAGAT CCGGCCAAAA
    GATGTCCCTG GGACACTGCT CAATATCGCG TTACTTAATT TAGGCAGTTC AGACCCTAGT
    TTACGGTCAG CTGCCTATAA TCTTCTGTGT GCCTTAACTT GTACCTTTAA TTTAAAAATC
    GAGGGCCAGT TGCTAGAGAC GTCAGGTTTA TGTATCCCTG CCAACAACAC CCTCTTTATT
    GTCTCTATTA GTAAGACACT GGCAGCCAAT GAGCCACACC TCACCTTAGA ATTTTTGGAA
    GAGTGTATTT CTGGATTTAG CAAATCTAGT ATTGAACTGA AACACCTTTG TTTGGAATAC
    ATGACTCCAT GGCTGTCAAA TCTAGTCCGT TTTTGTAAGC ACAATGATGA TGCCAAAAGA
    CAAAGAGTTA CTGCTATTCT TGATAAGCTG ATAACAATGA CCATAAATGA GAAACAGATG
    TACCCATCTA TTCAAGCAAA AATATGGGGG AGCCTTGGGC AGATTACAGA TCTCCTTGAT
    GTTGTGCTAG ACAGTTTCAT CAAAACCAGT GCAACAGGTG GCTTGGGATC AATAAAAGCC
    GAGGTGATGG CAGATACTGC TGTAGCTCTG GCGTCTGGAA ATGTGAAATT GGTTTCTAGC
    AAGGTTATTG GAAGGATGTG CAAAATAATT GACAAGACAT GCTTATCTCC AACTCCTACT
    TTAGAACAGC ATCTTATGTG GGATGACATT GCGATTCTAG CGCGCTACAT GCTGATGTTG
    TCCTTCAACA ACTCCCTCGA CGTGGCGGCT CATCTCCCCT ACCTCTTCCA CGTTGTGACT
    TTCTTAGTAG CCACAGGGCC ACTCTCCCTT AGAGCTTCCA CACATGGATT GGTCATTAAT
    ATCATTCACT CTCTGTGCAC TTGTTCACAG CTTCATTTTA GTGAGGAGAC CAAGCAAGTT
    TTGAGACTCA GTTTGACAGA GTTCTCATTA CCCAAATTTT ACTTACTGTT TGGCATTAGC
    AAAGTCAAGT CAGCTGCTGT GATTGCCTTC CGCTCCAGTT ACCGGGACAG GTCGTTCTCT
    CCTGGCTCCT ACGAGAGGGA GACTTTTGCT TTGACATCCT TGGAAACAGT CACGGAAGCT
    TTGTTGGAGA TCATGGAGGC ATGTATGAGA GATATTCCAA CATGCAAGTG GCTGGATCAG
    TGGACAGAAC TAGCTCAAAG GTTTGCATTC CAGTACAACC CGTCCCTGCA ACCGAGAGCT
    CTGGTCGTCT TTGGCTGCAT TAGTAAGCGA GTGTCTCACG GGCAGATAAA GCAGATTATC
    CGCATTCTTA GCAAGGCGCT TGAGAGTTGC TTAAAAGGAC CTGATACTTA CAACAGTCAA
    GTTCTGATAG AAGCTACAGT AATAGCACTA ACCAAATTAC AACCACTTCT TAATAAGGAC
    TCCCCTCTGC ACAAAGCCCT CTTTTGGGTA GCTGTGGCCG TGCTGCAGCT CGATGAGGTC
    AACTTGTATT CAGCAGGCAC CGCACTTCTT GAGCAAAACC TGCACACCTT AGACAGTCTC
    CGGATATTCA ATGACAAGAG TCCAGAGGAA GTATTTATGG CAATTCGGAA TCCTCTGGAA
    TGGCACTGCA AGCAAATGGA TCATTTTGTT GGACTCAATT TCAACTCTAA CTTTAACTTT
    GCACTGGTTG GACACCTCTT AAAAGGGTAC AGACATCCTT CACCTGCTAT TGTTGCACGA
    ACAGTCAGAA TTTTGCATAC ACTACTAACT CTGGTTAACA AACATAGAAA TTGTGACAAA
    TTTGAGGTGA ATACGCAGAG CGTGGCTTAC TTAGCAGCTT TACTCACAGT ATCTGAGGAG
    GTTCGAAGTC GCTGTAGCCT AAAACATAGA AAGTCTCTTC TTCTTACTGA TATTTCAATG
    GAAAATATTC CTATGGATAC ATATCCCATT CATCATGGCG ACCCCAGCTA TAGGACACTA
    AAGGAGAGTC AGCCATGGTC CTCTCCCAAA GGCTCGGAAG GGTACCTTGC AGCCACCTAT
    CCAACCGTGG GCCAGACCAG CCCCCGAGCC CGGAAATCCA TGAGCTTGGA CATGGGGCAG
    CCTTCTCAGG CCAACACTAA GAAGTTGCTG GGAACAAGGA AAAGTTTTGA TCACTTGATA
    TCAGACACGA AGGCTCCTAA AAGGCAAGAA GTGGAATCAG GGATCACAAC ACCCCCCAAA
    ATGAGGAGAG TAGCAGAAAC TGATTATGAA ATGGAAACTC AAAGGATTTC CTCATCACAA
    CAGCACCCAC ATTTACGTAA AGTTTCAGTG TCTGAATCAA ATGTTCTCTT GGATGAAGAA
    GTACTTACTG ACCCGAAGAT CCAGGCATTG CTGCTTACTG TTCTGGCTAC ACTGGTGAAA
    TACACCACGG ATGAGTTTGA TCAACGAATT CTTTATGAAT ACTTAGCAGA AGCCAGTGTT
    GTTTTCCCCA AAGTTTTTCC TGTTGTGCAC AATTTGTTGG ACTCCAAGAT CAACACCCTG
    TTGTCATTGT GCCAAGATCC AAATTTGTTA AATCCGATTC ATGGGATTGT GCAGAGTGTG
    GTATACTACG AGGAGTCCCC ACCACAGTAC CAGACGTCTT ACCTGCAGAG TTTTGGTTTT
    AATGGCTTGT GGCGGTTTGC AGGACCGTTT TCAAAGGAAT CGACAAGGAG AACGTTGAAC
    TCTCCCCAAC CACTGGGCAC TGTAACAGTG GACGAACTCG CCATGGATCC GCAAGCCAAG
    TGCAGAAGCA AAGAAGCGCT GGCAGTTTCA AACGTAATAG CATTAAGAAG ATCGTGTGAA
    GCTTTCTTTC TTTCCTTTTT CAAACAGACG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb45492
    Clone ID Related Accession (Same CDS sequence) XM_023653064.1
    Accession Version XM_023653064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofibromin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009154.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGAGATTAG ATGAAACAAT GCTGGTCAAA CAGTTGCTGC CAGAAATCTG CCATTTTCTT 
    CACGCCTGTC GTGAAGGAAA CCAACATGCA GCTGAACTTC GGAATTCTGC CTCTGGGGTT
    TTATTTTCTC TCAGTTGCAA CAACTTCAAT GCAGTCTTTA GTCGCATTTC TACCAGGTTA
    CAGGAATTGA CTGTTTGTTC AGAAGATAAT GTTGATGTTC ATGATATAGA ATTGTTACAG
    TATATCAATG TGGATTGTGC AAAATTAAAA CGACTCCTGA AGGAAACAGC ATTTAAATTT
    AAAGCCCTAA AGAAGGTTGC ACAGTTAGCA GTTATAAATA GCCTGGAAAA AGCATTTTGG
    AACTGGGTCG AAAATTATCC AGATGAATTT ACAAAGCTAT ACCAGATTCC ACAGACTGAT
    ATGGCTGAGT GTGCGGAAAA ACTGTTTGAC TTGGTGGATG GTTTTGCTGA AAGCACCAAA
    CGTAAAGCAG CAGTTTGGCC GCTTCAAATC ATTCTCCTTA TCTTGTGTCC AGAAATAATC
    CAGGATATAT CCAAGGATGT GGTTGATGAA AGCAACATGA ATAAGAAGTT GTTTCTGGAC
    AGTCTGCGGA AAGCGCTTGC TGGCCATGGA GGAAGCAGGC AGCTGACTGA AAGTGCCGCC
    ACTGCCTGTG TGAAGCTGTG CAAAGCGAGT ACTTACATCA ACTGGGAAGA CAACTCGGTC
    ATTTTCCTAC TAGTTCAGTC CATGGTGGTC GATCTTAAGA ACCTGCTTTT TAATCCAAGT
    AAACCATTCT CAAGAGGCAG TCAACCTGCA GACGTGGATC TAATGATTGA CTGCCTTGTT
    TCTTGCTTTC GTATAAGCCC TCACAACAAC CAACACTTTA AGATCTGCCT GGCTCAGAAT
    TCTCCTTCTA CGTTTCACTA TGTGCTGGTA AATTCACTCC ATCGAATCAT CACCAATAGC
    ACTTTCAAGC ATGGACTTGG CACTGCTTCT GCACTGGATT GGTGGCCTAA GATCGATGCC
    GTATATTGTC ACTCAGTTGA ACTTCGAAAT ATGTTTGGTG AAACACTTCA TAAAGCGGTG
    CAGGGTTGTG GAGCACACCC GGCGATACGC ATGGCACCGA GTCTTACATT TAAAGAAAAA
    GTAACAAGCC TTAAATTTAA AGAAAAACCT ACAGACCTGG AGACAAGAAG CTATAAGTAT
    CTTCTTTTGT CTATGGTGAA ACTAATTCAT GCAGATCCAA AGCTCTTGCT TTGTAATCCC
    AGAAAACAGG GGCCTGAAAC CCAAGGCAGT ACAGCAGAAT TAATTACAGG GCTCGTCCAG
    CTGGTCCCTC AGTCACACAT GCCAGAGATT GCTCAGGAAG CAATGGAGGC TCTGCTGGTG
    CTTCATCAGT TAGACAGCAT CGATTTGTGG AATCCTGATG CTCCTGTGGA AACATTTTGG
    GAAATTAGCT CACAAATGCT TTTTTACATC TGCAAGAAGT TAACTAGCCA GCAAATGCTT
    AGTAGCACAG AAATTCTCAA ATGGTTGCGG GAAATTTTGA TCTGCAGGAA TAAATTTCTT
    CTTAAAAATA AGCAGGCAGA TAGGAGTTCC TGTCACTTTC TCTTCCTTTA TGGGGTAGGA
    TGTGATGTTC CTTCCAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAGTTATTA
    CGTACTTATA CTCCTGGAGC CTCTCTCCGG AAGGGAAAAG GAAACTCATC CATGGATAGT
    GCGGCAGGGT GCAGTGGAAC GCCGCCGATA TGCCGGCAAG CCCAGACCAA ACTGGAAGTG
    GCCCTGTACA TGTTTCTGTG GAGCCCTGAC ACCGAAGCTG TTCTGGTTGC CATGTCCTGT
    TTCCGCCACC TCTGTGAGGA AGCGGATATC CGGTGTGGGG TGGACGAAGT ATCGGTGCAC
    AACTTCTTGC CGAACTATAA CACATTCATG GAATTTGCGT CTGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCACTTCA GAAAAGAGTG ATGGCACTAC TGAGGCGCAT TGAACATCCC
    ACTGCAGGAA ACACTGAGGC TTGGGAAGAT ACACATGCAA AGTGGGAACA AGCTACAAAA
    CTAATCCTCA ACTATCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCTGA AAGCCTTCAT
    AAGACCATTG TTAAGAGGCG AATGTCTCAT GTGAGTGGAG GAGGATCCAT AGATTTGTCT
    GACACGGACT CCCTTCAGGA ATGGATCAAC ATGACCGGCT TCCTCTGTGC CCTTGGGGGA
    GTGTGCCTGC AGCAGAGAAG CAATTCTGGC CTGGCAACCT ATAGCCCACC CATGGGCCCA
    GTCAGTGAAC GCAAGGGTTC CATGATTTCA GTGATGTCCT CAGAGGGAAA TGCAGACACA
    CCTGTCAGCA AATTTATGGA TCGGCTGCTA TCCTTAATGG TGTGTAACCA TGAGAAAGTG
    GGACTTCAAA TACGCACCAA CGTTAAGGAT CTGGTGGGTT TAGAATTGAG TCCTGCTCTT
    TATCCAATGC TGTTTAACAA ATTGAAGAAC ACCATCAGCA AGTTTTTTGA CTCCCAAGGA
    CAGGTTTTAT TGACTGACAC CAATACTCAA TTTGTAGAGC AAACCATAGC TATAATGAAG
    AACTTACTAG ATAATCATAC TGAAGGCAGC TCCGAACATC TGGGGCAAGC TAGCATTGAA
    ACGATGATGT TAAATCTGGT CAGATATGTT CGTGTACTTG GGAACATGGT CCATGCAATT
    CAAATAAAAA CGAAACTGTG TCAGCTAGTC GAGGTAATGA TGGCAAGAAG AGATGACCTC
    TCATTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTGAC AGATTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GATGTAAAAT GTCTTACAAG AGATCTGGAC
    CAGGCAAGCA TGGAAGCAGT AGTTTCACTC CTGGCTGGTC TCCCGCTGCA GCCTGAGGAA
    GGAGATGGTG TGGAACTCAT GGAAGCCAAG TCACAGCTAT TTCTTAAGTA TTTCACATTG
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACAGGTGGC
    AGGAAGCGTG GCATGTCTCG GAGGCTGGCC TCACTGAGGC ACTGCACAGT CCTTGCAATG
    TCAAACTTAC TTAACGCTAA CGTGGACAGT GGCCTCATGC ACTCCATAGG TTTAGGTTAT
    CACAAAGATC TCCAGACAAG AGCTACATTT ATGGAAGTTC TGACAAAAAT CCTCCAACAA
    GGCACAGAAT TTGACACACT TGCAGAAACG GTGTTGGCAG ATCGGTTTGA GAGGCTGGTG
    GAGTTGGTCA CAATGATGGG TGATCAGGGA GAGCTCCCTA TAGCCATGGC TCTGGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAACTA GCTCGAGTTC TAGTGACTCT CTTTGATTCT
    CGGCATTTAC TCTACCAACT GCTCTGGAAC ATGTTTTCTA AGGAAGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTTTTCCG AGGCAACAGC TTAGCCAGTA AAATAATGAC CTTCTGTTTC
    AAGGTATATG GTGCTACCTA CCTACAAAAA CTCCTGGATC CTTTGTTACG AATTGTGATC
    ACATCCTCTG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACAGTCA
    GAGAGCCTTG AGGAGAACCA GCGGAGCCTC CTTCAGATGA CTGAAAAGTT CTTCCACGCC
    ATCATCAGTT CCTCCTCGGA ATTCCCCCCT CAGCTTCGAA GTGTGTGCCA CTGTCTATAC
    CAGGCAACTT GCCACTCCCT ACTGAATACA GCTACAGTAA AAGAAAAAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG TTTCCCTCAG AACAGCATCG GTGCAGTGGG AAGTGCCATG
    TTCCTCAGAT TCATCAATCC TGCCATCGTC TCACCGTATG AAGCAGGCAT TTTAGATAAA
    AAGCCACCAC CTAGAATCGA AAGGGGCTTG AAGTTAATGT CAAAGATACT TCAGAGTATT
    GCCAATCATG TTCTCTTCAC AAAAGAAGAG CATATGCGGC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ATGCAGCACG AAGGTTTTTC CTTGATATAG CATCCGATTG TCCTACAAGT
    GATGCAGTGA ATCACAGTCT TTCCTTCATC AGCGATGGCA ACGTGCTGGC TCTACATCGT
    CTCCTCTGGA ACAATCAAGA GAAGATTGGC CAGTATCTTT CCAGCAATAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCAACACTTC TTGCATACCT GGGTCCTCCA
    GAGCACAAAC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTC
    GAGGAATTTA TGACTAGGCA TCAAGTACAT GAAAAAGAAG AGTTCAAGGC CTTGAAAACA
    TTAAGTATTT TCTACCAAGC TGGGACTTCC AGAGCTGGGA ATCCTATATT TTATTATGTT
    GCACGGAGGT TCAAAACTGG TCAAATCAAT GGCGATTTGC TGATCTACCA TGTCTTGCTG
    ACTCTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACCGGG
    CCTAGCAATC GCTTTAAAAC GGACTTTCTC TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT
    GCTTATGACA ATGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAC
    ACCAAGTATC ACGAGCGGCT GCTCACTGGC CTCAAAGGCA GCAAAAGGCT CATTTTCATA
    GACTGTCCTG GGAAGCTGGC TGAGCACGTA GAGCACGAAC AACAGAAACT ACCTGCTGCC
    ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG TTCCACAATG CTCTCAAGCT GGCTCACAAA
    GACACCAAGG TTTCCATTAA AGTTGGTTCT ACTGCTGTTC AAGTGACCTC AGCAGAGCGA
    ACGAAAGTCC TAGGGCAATC AGTCTTTCTA AACGATATCT ACTATGCTTC GGAAATTGAA
    GAGATCTGCC TGGTGGATGA GAACCAGTTC ACCTTAACCA TTGCAAACCA GGGCACACCG
    CTCACCTTCA TGCACCAGGA GTGTGAAGCC ATCGTCCAGT CCATCATTCA CATCCGGACA
    CGCTGGGAGC TGTCACAGCC CGACTCCATC CCCCAGCACA CCAAGATCCG GCCAAAAGAT
    GTCCCTGGGA CACTGCTCAA TATCGCGTTA CTTAATTTAG GCAGTTCAGA CCCTAGTTTA
    CGGTCAGCTG CCTATAATCT TCTGTGTGCC TTAACTTGTA CCTTTAATTT AAAAATCGAG
    GGCCAGTTGC TAGAGACGTC AGGTTTATGT ATCCCTGCCA ACAACACCCT CTTTATTGTC
    TCTATTAGTA AGACACTGGC AGCCAATGAG CCACACCTCA CCTTAGAATT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAACTGAAAC ACCTTTGTTT GGAATACATG
    ACTCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCACA ATGATGATGC CAAAAGACAA
    AGAGTTACTG CTATTCTTGA TAAGCTGATA ACAATGACCA TAAATGAGAA ACAGATGTAC
    CCATCTATTC AAGCAAAAAT ATGGGGGAGC CTTGGGCAGA TTACAGATCT CCTTGATGTT
    GTGCTAGACA GTTTCATCAA AACCAGTGCA ACAGGTGGCT TGGGATCAAT AAAAGCCGAG
    GTGATGGCAG ATACTGCTGT AGCTCTGGCG TCTGGAAATG TGAAATTGGT TTCTAGCAAG
    GTTATTGGAA GGATGTGCAA AATAATTGAC AAGACATGCT TATCTCCAAC TCCTACTTTA
    GAACAGCATC TTATGTGGGA TGACATTGCG ATTCTAGCGC GCTACATGCT GATGTTGTCC
    TTCAACAACT CCCTCGACGT GGCGGCTCAT CTCCCCTACC TCTTCCACGT TGTGACTTTC
    TTAGTAGCCA CAGGGCCACT CTCCCTTAGA GCTTCCACAC ATGGATTGGT CATTAATATC
    ATTCACTCTC TGTGCACTTG TTCACAGCTT CATTTTAGTG AGGAGACCAA GCAAGTTTTG
    AGACTCAGTT TGACAGAGTT CTCATTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC TCCAGTTACC GGGACAGGTC GTTCTCTCCT
    GGCTCCTACG AGAGGGAGAC TTTTGCTTTG ACATCCTTGG AAACAGTCAC GGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGGTT TGCATTCCAG TACAACCCGT CCCTGCAACC GAGAGCTCTG
    GTCGTCTTTG GCTGCATTAG TAAGCGAGTG TCTCACGGGC AGATAAAGCA GATTATCCGC
    ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA AAAGGACCTG ATACTTACAA CAGTCAAGTT
    CTGATAGAAG CTACAGTAAT AGCACTAACC AAATTACAAC CACTTCTTAA TAAGGACTCC
    CCTCTGCACA AAGCCCTCTT TTGGGTAGCT GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC
    TTGTATTCAG CAGGCACCGC ACTTCTTGAG CAAAACCTGC ACACCTTAGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC AGAGGAAGTA TTTATGGCAA TTCGGAATCC TCTGGAATGG
    CACTGCAAGC AAATGGATCA TTTTGTTGGA CTCAATTTCA ACTCTAACTT TAACTTTGCA
    CTGGTTGGAC ACCTCTTAAA AGGGTACAGA CATCCTTCAC CTGCTATTGT TGCACGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTG GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CGCAGAGCGT GGCTTACTTA GCAGCTTTAC TCACAGTATC TGAGGAGGTT
    CGAAGTCGCT GTAGCCTAAA ACATAGAAAG TCTCTTCTTC TTACTGATAT TTCAATGGAA
    AATATTCCTA TGGATACATA TCCCATTCAT CATGGCGACC CCAGCTATAG GACACTAAAG
    GAGAGTCAGC CATGGTCCTC TCCCAAAGGC TCGGAAGGGT ACCTTGCAGC CACCTATCCA
    ACCGTGGGCC AGACCAGCCC CCGAGCCCGG AAATCCATGA GCTTGGACAT GGGGCAGCCT
    TCTCAGGCCA ACACTAAGAA GTTGCTGGGA ACAAGGAAAA GTTTTGATCA CTTGATATCA
    GACACGAAGG CTCCTAAAAG GCAAGAAGTG GAATCAGGGA TCACAACACC CCCCAAAATG
    AGGAGAGTAG CAGAAACTGA TTATGAAATG GAAACTCAAA GGATTTCCTC ATCACAACAG
    CACCCACATT TACGTAAAGT TTCAGTGTCT GAATCAAATG TTCTCTTGGA TGAAGAAGTA
    CTTACTGACC CGAAGATCCA GGCATTGCTG CTTACTGTTC TGGCTACACT GGTGAAATAC
    ACCACGGATG AGTTTGATCA ACGAATTCTT TATGAATACT TAGCAGAAGC CAGTGTTGTT
    TTCCCCAAAG TTTTTCCTGT TGTGCACAAT TTGTTGGACT CCAAGATCAA CACCCTGTTG
    TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCGATTCATG GGATTGTGCA GAGTGTGGTA
    TACTACGAGG AGTCCCCACC ACAGTACCAG ACGTCTTACC TGCAGAGTTT TGGTTTTAAT
    GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG
    CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTATT TGCCTGGAAT TGATGAAGAA
    ACCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTATC CTCCTGCAGT GCAGAGCCAG
    CTTAGTATTA CTGCTAACCT TAACCTTTCC AATTCCATGA CCTCACTTGC AACTTCCCAG
    CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CAACCACTGG GCACTGTAAC
    AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT
    TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023508832.1
    CDS34..8286
    Translation

    Target ORF information:

    RefSeq Version XM_023653064.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofibromin 1 (NF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023653064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGAGATTAG ATGAAACAAT GCTGGTCAAA CAGTTGCTGC CAGAAATCTG CCATTTTCTT 
    CACGCCTGTC GTGAAGGAAA CCAACATGCA GCTGAACTTC GGAATTCTGC CTCTGGGGTT
    TTATTTTCTC TCAGTTGCAA CAACTTCAAT GCAGTCTTTA GTCGCATTTC TACCAGGTTA
    CAGGAATTGA CTGTTTGTTC AGAAGATAAT GTTGATGTTC ATGATATAGA ATTGTTACAG
    TATATCAATG TGGATTGTGC AAAATTAAAA CGACTCCTGA AGGAAACAGC ATTTAAATTT
    AAAGCCCTAA AGAAGGTTGC ACAGTTAGCA GTTATAAATA GCCTGGAAAA AGCATTTTGG
    AACTGGGTCG AAAATTATCC AGATGAATTT ACAAAGCTAT ACCAGATTCC ACAGACTGAT
    ATGGCTGAGT GTGCGGAAAA ACTGTTTGAC TTGGTGGATG GTTTTGCTGA AAGCACCAAA
    CGTAAAGCAG CAGTTTGGCC GCTTCAAATC ATTCTCCTTA TCTTGTGTCC AGAAATAATC
    CAGGATATAT CCAAGGATGT GGTTGATGAA AGCAACATGA ATAAGAAGTT GTTTCTGGAC
    AGTCTGCGGA AAGCGCTTGC TGGCCATGGA GGAAGCAGGC AGCTGACTGA AAGTGCCGCC
    ACTGCCTGTG TGAAGCTGTG CAAAGCGAGT ACTTACATCA ACTGGGAAGA CAACTCGGTC
    ATTTTCCTAC TAGTTCAGTC CATGGTGGTC GATCTTAAGA ACCTGCTTTT TAATCCAAGT
    AAACCATTCT CAAGAGGCAG TCAACCTGCA GACGTGGATC TAATGATTGA CTGCCTTGTT
    TCTTGCTTTC GTATAAGCCC TCACAACAAC CAACACTTTA AGATCTGCCT GGCTCAGAAT
    TCTCCTTCTA CGTTTCACTA TGTGCTGGTA AATTCACTCC ATCGAATCAT CACCAATAGC
    ACTTTCAAGC ATGGACTTGG CACTGCTTCT GCACTGGATT GGTGGCCTAA GATCGATGCC
    GTATATTGTC ACTCAGTTGA ACTTCGAAAT ATGTTTGGTG AAACACTTCA TAAAGCGGTG
    CAGGGTTGTG GAGCACACCC GGCGATACGC ATGGCACCGA GTCTTACATT TAAAGAAAAA
    GTAACAAGCC TTAAATTTAA AGAAAAACCT ACAGACCTGG AGACAAGAAG CTATAAGTAT
    CTTCTTTTGT CTATGGTGAA ACTAATTCAT GCAGATCCAA AGCTCTTGCT TTGTAATCCC
    AGAAAACAGG GGCCTGAAAC CCAAGGCAGT ACAGCAGAAT TAATTACAGG GCTCGTCCAG
    CTGGTCCCTC AGTCACACAT GCCAGAGATT GCTCAGGAAG CAATGGAGGC TCTGCTGGTG
    CTTCATCAGT TAGACAGCAT CGATTTGTGG AATCCTGATG CTCCTGTGGA AACATTTTGG
    GAAATTAGCT CACAAATGCT TTTTTACATC TGCAAGAAGT TAACTAGCCA GCAAATGCTT
    AGTAGCACAG AAATTCTCAA ATGGTTGCGG GAAATTTTGA TCTGCAGGAA TAAATTTCTT
    CTTAAAAATA AGCAGGCAGA TAGGAGTTCC TGTCACTTTC TCTTCCTTTA TGGGGTAGGA
    TGTGATGTTC CTTCCAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAGTTATTA
    CGTACTTATA CTCCTGGAGC CTCTCTCCGG AAGGGAAAAG GAAACTCATC CATGGATAGT
    GCGGCAGGGT GCAGTGGAAC GCCGCCGATA TGCCGGCAAG CCCAGACCAA ACTGGAAGTG
    GCCCTGTACA TGTTTCTGTG GAGCCCTGAC ACCGAAGCTG TTCTGGTTGC CATGTCCTGT
    TTCCGCCACC TCTGTGAGGA AGCGGATATC CGGTGTGGGG TGGACGAAGT ATCGGTGCAC
    AACTTCTTGC CGAACTATAA CACATTCATG GAATTTGCGT CTGTCAGCAA TATGATGTCA
    ACAGGAAGAG CAGCACTTCA GAAAAGAGTG ATGGCACTAC TGAGGCGCAT TGAACATCCC
    ACTGCAGGAA ACACTGAGGC TTGGGAAGAT ACACATGCAA AGTGGGAACA AGCTACAAAA
    CTAATCCTCA ACTATCCAAA AGCCAAAATG GAAGATGGCC AGGCTGCTGA AAGCCTTCAT
    AAGACCATTG TTAAGAGGCG AATGTCTCAT GTGAGTGGAG GAGGATCCAT AGATTTGTCT
    GACACGGACT CCCTTCAGGA ATGGATCAAC ATGACCGGCT TCCTCTGTGC CCTTGGGGGA
    GTGTGCCTGC AGCAGAGAAG CAATTCTGGC CTGGCAACCT ATAGCCCACC CATGGGCCCA
    GTCAGTGAAC GCAAGGGTTC CATGATTTCA GTGATGTCCT CAGAGGGAAA TGCAGACACA
    CCTGTCAGCA AATTTATGGA TCGGCTGCTA TCCTTAATGG TGTGTAACCA TGAGAAAGTG
    GGACTTCAAA TACGCACCAA CGTTAAGGAT CTGGTGGGTT TAGAATTGAG TCCTGCTCTT
    TATCCAATGC TGTTTAACAA ATTGAAGAAC ACCATCAGCA AGTTTTTTGA CTCCCAAGGA
    CAGGTTTTAT TGACTGACAC CAATACTCAA TTTGTAGAGC AAACCATAGC TATAATGAAG
    AACTTACTAG ATAATCATAC TGAAGGCAGC TCCGAACATC TGGGGCAAGC TAGCATTGAA
    ACGATGATGT TAAATCTGGT CAGATATGTT CGTGTACTTG GGAACATGGT CCATGCAATT
    CAAATAAAAA CGAAACTGTG TCAGCTAGTC GAGGTAATGA TGGCAAGAAG AGATGACCTC
    TCATTTTGTC AAGAGATGAA ATTTAGGAAT AAGATGGTAG AATATCTGAC AGATTGGGTT
    ATGGGAACAT CAAACCAAGC AGCAGATGAT GATGTAAAAT GTCTTACAAG AGATCTGGAC
    CAGGCAAGCA TGGAAGCAGT AGTTTCACTC CTGGCTGGTC TCCCGCTGCA GCCTGAGGAA
    GGAGATGGTG TGGAACTCAT GGAAGCCAAG TCACAGCTAT TTCTTAAGTA TTTCACATTG
    TTTATGAACC TTTTGAATGA CTGTAGTGAA GTTGAAGATG AAAATGCACA AACAGGTGGC
    AGGAAGCGTG GCATGTCTCG GAGGCTGGCC TCACTGAGGC ACTGCACAGT CCTTGCAATG
    TCAAACTTAC TTAACGCTAA CGTGGACAGT GGCCTCATGC ACTCCATAGG TTTAGGTTAT
    CACAAAGATC TCCAGACAAG AGCTACATTT ATGGAAGTTC TGACAAAAAT CCTCCAACAA
    GGCACAGAAT TTGACACACT TGCAGAAACG GTGTTGGCAG ATCGGTTTGA GAGGCTGGTG
    GAGTTGGTCA CAATGATGGG TGATCAGGGA GAGCTCCCTA TAGCCATGGC TCTGGCCAAT
    GTGGTCCCTT GTTCTCAGTG GGATGAACTA GCTCGAGTTC TAGTGACTCT CTTTGATTCT
    CGGCATTTAC TCTACCAACT GCTCTGGAAC ATGTTTTCTA AGGAAGTAGA ATTGGCAGAC
    TCCATGCAGA CTCTTTTCCG AGGCAACAGC TTAGCCAGTA AAATAATGAC CTTCTGTTTC
    AAGGTATATG GTGCTACCTA CCTACAAAAA CTCCTGGATC CTTTGTTACG AATTGTGATC
    ACATCCTCTG ATTGGCAACA TGTTAGCTTT GAAGTGGATC CTACCAGGTT AGAACAGTCA
    GAGAGCCTTG AGGAGAACCA GCGGAGCCTC CTTCAGATGA CTGAAAAGTT CTTCCACGCC
    ATCATCAGTT CCTCCTCGGA ATTCCCCCCT CAGCTTCGAA GTGTGTGCCA CTGTCTATAC
    CAGGCAACTT GCCACTCCCT ACTGAATACA GCTACAGTAA AAGAAAAAAA GGAAAACAAA
    AAATCAGTGG TTAGCCAGCG TTTCCCTCAG AACAGCATCG GTGCAGTGGG AAGTGCCATG
    TTCCTCAGAT TCATCAATCC TGCCATCGTC TCACCGTATG AAGCAGGCAT TTTAGATAAA
    AAGCCACCAC CTAGAATCGA AAGGGGCTTG AAGTTAATGT CAAAGATACT TCAGAGTATT
    GCCAATCATG TTCTCTTCAC AAAAGAAGAG CATATGCGGC CTTTTAATGA TTTTGTGAAA
    AGCAACTTTG ATGCAGCACG AAGGTTTTTC CTTGATATAG CATCCGATTG TCCTACAAGT
    GATGCAGTGA ATCACAGTCT TTCCTTCATC AGCGATGGCA ACGTGCTGGC TCTACATCGT
    CTCCTCTGGA ACAATCAAGA GAAGATTGGC CAGTATCTTT CCAGCAATAG GGATCATAAA
    GCTGTTGGAA GACGACCTTT TGATAAGATG GCAACACTTC TTGCATACCT GGGTCCTCCA
    GAGCACAAAC CTGTGGCAGA TACACACTGG TCCAGCCTTA ACCTTACCAG TTCAAAGTTC
    GAGGAATTTA TGACTAGGCA TCAAGTACAT GAAAAAGAAG AGTTCAAGGC CTTGAAAACA
    TTAAGTATTT TCTACCAAGC TGGGACTTCC AGAGCTGGGA ATCCTATATT TTATTATGTT
    GCACGGAGGT TCAAAACTGG TCAAATCAAT GGCGATTTGC TGATCTACCA TGTCTTGCTG
    ACTCTAAAGC CATATTATGC AAAGCCATAT GAAATTGTAG TGGACCTTAC CCATACCGGG
    CCTAGCAATC GCTTTAAAAC GGACTTTCTC TCCAAGTGGT TTGTTGTTTT TCCTGGCTTT
    GCTTATGACA ATGTCTCTGC AGTCTATATC TATAACTGTA ACTCCTGGGT CAGGGAGTAC
    ACCAAGTATC ACGAGCGGCT GCTCACTGGC CTCAAAGGCA GCAAAAGGCT CATTTTCATA
    GACTGTCCTG GGAAGCTGGC TGAGCACGTA GAGCACGAAC AACAGAAACT ACCTGCTGCC
    ACTTTGGCTT TAGAAGAGGA CCTGAAAGTG TTCCACAATG CTCTCAAGCT GGCTCACAAA
    GACACCAAGG TTTCCATTAA AGTTGGTTCT ACTGCTGTTC AAGTGACCTC AGCAGAGCGA
    ACGAAAGTCC TAGGGCAATC AGTCTTTCTA AACGATATCT ACTATGCTTC GGAAATTGAA
    GAGATCTGCC TGGTGGATGA GAACCAGTTC ACCTTAACCA TTGCAAACCA GGGCACACCG
    CTCACCTTCA TGCACCAGGA GTGTGAAGCC ATCGTCCAGT CCATCATTCA CATCCGGACA
    CGCTGGGAGC TGTCACAGCC CGACTCCATC CCCCAGCACA CCAAGATCCG GCCAAAAGAT
    GTCCCTGGGA CACTGCTCAA TATCGCGTTA CTTAATTTAG GCAGTTCAGA CCCTAGTTTA
    CGGTCAGCTG CCTATAATCT TCTGTGTGCC TTAACTTGTA CCTTTAATTT AAAAATCGAG
    GGCCAGTTGC TAGAGACGTC AGGTTTATGT ATCCCTGCCA ACAACACCCT CTTTATTGTC
    TCTATTAGTA AGACACTGGC AGCCAATGAG CCACACCTCA CCTTAGAATT TTTGGAAGAG
    TGTATTTCTG GATTTAGCAA ATCTAGTATT GAACTGAAAC ACCTTTGTTT GGAATACATG
    ACTCCATGGC TGTCAAATCT AGTCCGTTTT TGTAAGCACA ATGATGATGC CAAAAGACAA
    AGAGTTACTG CTATTCTTGA TAAGCTGATA ACAATGACCA TAAATGAGAA ACAGATGTAC
    CCATCTATTC AAGCAAAAAT ATGGGGGAGC CTTGGGCAGA TTACAGATCT CCTTGATGTT
    GTGCTAGACA GTTTCATCAA AACCAGTGCA ACAGGTGGCT TGGGATCAAT AAAAGCCGAG
    GTGATGGCAG ATACTGCTGT AGCTCTGGCG TCTGGAAATG TGAAATTGGT TTCTAGCAAG
    GTTATTGGAA GGATGTGCAA AATAATTGAC AAGACATGCT TATCTCCAAC TCCTACTTTA
    GAACAGCATC TTATGTGGGA TGACATTGCG ATTCTAGCGC GCTACATGCT GATGTTGTCC
    TTCAACAACT CCCTCGACGT GGCGGCTCAT CTCCCCTACC TCTTCCACGT TGTGACTTTC
    TTAGTAGCCA CAGGGCCACT CTCCCTTAGA GCTTCCACAC ATGGATTGGT CATTAATATC
    ATTCACTCTC TGTGCACTTG TTCACAGCTT CATTTTAGTG AGGAGACCAA GCAAGTTTTG
    AGACTCAGTT TGACAGAGTT CTCATTACCC AAATTTTACT TACTGTTTGG CATTAGCAAA
    GTCAAGTCAG CTGCTGTGAT TGCCTTCCGC TCCAGTTACC GGGACAGGTC GTTCTCTCCT
    GGCTCCTACG AGAGGGAGAC TTTTGCTTTG ACATCCTTGG AAACAGTCAC GGAAGCTTTG
    TTGGAGATCA TGGAGGCATG TATGAGAGAT ATTCCAACAT GCAAGTGGCT GGATCAGTGG
    ACAGAACTAG CTCAAAGGTT TGCATTCCAG TACAACCCGT CCCTGCAACC GAGAGCTCTG
    GTCGTCTTTG GCTGCATTAG TAAGCGAGTG TCTCACGGGC AGATAAAGCA GATTATCCGC
    ATTCTTAGCA AGGCGCTTGA GAGTTGCTTA AAAGGACCTG ATACTTACAA CAGTCAAGTT
    CTGATAGAAG CTACAGTAAT AGCACTAACC AAATTACAAC CACTTCTTAA TAAGGACTCC
    CCTCTGCACA AAGCCCTCTT TTGGGTAGCT GTGGCCGTGC TGCAGCTCGA TGAGGTCAAC
    TTGTATTCAG CAGGCACCGC ACTTCTTGAG CAAAACCTGC ACACCTTAGA CAGTCTCCGG
    ATATTCAATG ACAAGAGTCC AGAGGAAGTA TTTATGGCAA TTCGGAATCC TCTGGAATGG
    CACTGCAAGC AAATGGATCA TTTTGTTGGA CTCAATTTCA ACTCTAACTT TAACTTTGCA
    CTGGTTGGAC ACCTCTTAAA AGGGTACAGA CATCCTTCAC CTGCTATTGT TGCACGAACA
    GTCAGAATTT TGCATACACT ACTAACTCTG GTTAACAAAC ATAGAAATTG TGACAAATTT
    GAGGTGAATA CGCAGAGCGT GGCTTACTTA GCAGCTTTAC TCACAGTATC TGAGGAGGTT
    CGAAGTCGCT GTAGCCTAAA ACATAGAAAG TCTCTTCTTC TTACTGATAT TTCAATGGAA
    AATATTCCTA TGGATACATA TCCCATTCAT CATGGCGACC CCAGCTATAG GACACTAAAG
    GAGAGTCAGC CATGGTCCTC TCCCAAAGGC TCGGAAGGGT ACCTTGCAGC CACCTATCCA
    ACCGTGGGCC AGACCAGCCC CCGAGCCCGG AAATCCATGA GCTTGGACAT GGGGCAGCCT
    TCTCAGGCCA ACACTAAGAA GTTGCTGGGA ACAAGGAAAA GTTTTGATCA CTTGATATCA
    GACACGAAGG CTCCTAAAAG GCAAGAAGTG GAATCAGGGA TCACAACACC CCCCAAAATG
    AGGAGAGTAG CAGAAACTGA TTATGAAATG GAAACTCAAA GGATTTCCTC ATCACAACAG
    CACCCACATT TACGTAAAGT TTCAGTGTCT GAATCAAATG TTCTCTTGGA TGAAGAAGTA
    CTTACTGACC CGAAGATCCA GGCATTGCTG CTTACTGTTC TGGCTACACT GGTGAAATAC
    ACCACGGATG AGTTTGATCA ACGAATTCTT TATGAATACT TAGCAGAAGC CAGTGTTGTT
    TTCCCCAAAG TTTTTCCTGT TGTGCACAAT TTGTTGGACT CCAAGATCAA CACCCTGTTG
    TCATTGTGCC AAGATCCAAA TTTGTTAAAT CCGATTCATG GGATTGTGCA GAGTGTGGTA
    TACTACGAGG AGTCCCCACC ACAGTACCAG ACGTCTTACC TGCAGAGTTT TGGTTTTAAT
    GGCTTGTGGC GGTTTGCAGG ACCGTTTTCA AAGCAAACAC AAATCCCAGA CTATGCTGAG
    CTTATTGTTA AGTTTCTTGA TGCCTTGATT GACACGTATT TGCCTGGAAT TGATGAAGAA
    ACCAGTGAGG AGTCCCTCCT GACACCCACA TCTCCTTATC CTCCTGCAGT GCAGAGCCAG
    CTTAGTATTA CTGCTAACCT TAACCTTTCC AATTCCATGA CCTCACTTGC AACTTCCCAG
    CATTCCCCAG GAATCGACAA GGAGAACGTT GAACTCTCCC CAACCACTGG GCACTGTAAC
    AGTGGACGAA CTCGCCATGG ATCCGCAAGC CAAGTGCAGA AGCAAAGAAG CGCTGGCAGT
    TTCAAACGTA ATAGCATTAA GAAGATCGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.