MCF2L gene cDNA ORF clone, Equus caballus(horse)

mRNA	Protein	Name
XM_005601348.2	XP_005601405.1	guanine nucleotide exchange factor DBS isoform X3
XM_005601350.2	XP_005601407.1	guanine nucleotide exchange factor DBS isoform X6
XM_023621869.1	XP_023477637.1	guanine nucleotide exchange factor DBS isoform X7
XM_014732205.1	XP_014587691.1	guanine nucleotide exchange factor DBS isoform X1
XM_014732206.1	XP_014587692.1	guanine nucleotide exchange factor DBS isoform X4
XM_023621862.1	XP_023477630.1	guanine nucleotide exchange factor DBS isoform X1
XM_023621863.1	XP_023477631.1	guanine nucleotide exchange factor DBS isoform X2
XM_023621870.1	XP_023477638.1	guanine nucleotide exchange factor DBS isoform X8
XM_023621871.1	XP_023477639.1	guanine nucleotide exchange factor DBS isoform X9
XM_014732205.2	XP_014587691.2	guanine nucleotide exchange factor DBS isoform X10
XM_014732207.1	XP_014587693.1	guanine nucleotide exchange factor DBS isoform X5
XM_014732208.1	XP_014587694.1	guanine nucleotide exchange factor DBS isoform X5
XM_023621866.1	XP_023477634.1	guanine nucleotide exchange factor DBS isoform X5
XM_005601348.3	XP_005601405.2	guanine nucleotide exchange factor DBS isoform X12
XM_023621873.1	XP_023477641.1	guanine nucleotide exchange factor DBS isoform X15
XM_005601347.2	XP_005601404.1	guanine nucleotide exchange factor DBS isoform X2
XM_014732209.1	XP_014587695.1	guanine nucleotide exchange factor DBS isoform X5
XM_023621864.1	XP_023477632.1	guanine nucleotide exchange factor DBS isoform X3
XM_023621867.1	XP_023477635.1	guanine nucleotide exchange factor DBS isoform X6
XM_005601347.3	XP_005601404.2	guanine nucleotide exchange factor DBS isoform X11
XM_023621872.1	XP_023477640.1	guanine nucleotide exchange factor DBS isoform X13
XM_014732206.2	XP_014587692.2	guanine nucleotide exchange factor DBS isoform X14
XM_023621865.1	XP_023477633.1	guanine nucleotide exchange factor DBS isoform X4
XM_023621868.1	XP_023477636.1	guanine nucleotide exchange factor DBS isoform X7
XM_014732209.2	XP_014587695.2	guanine nucleotide exchange factor DBS isoform X15
XM_005601350.3	XP_005601407.2	guanine nucleotide exchange factor DBS isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb36104	XM_014732205.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OHb36105	XM_014732206.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHb36106	XM_014732209.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHb36106	XM_014732207.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHb36106	XM_014732208.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHb52006	XM_023621862.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52007	XM_023621863.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52008	XM_023621864.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52009	XM_023621865.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52010	XM_023621866.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52011	XM_023621867.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52012	XM_023621869.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52012	XM_023621868.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52013	XM_023621870.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52014	XM_023621871.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52015	XM_014732205.2 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52016	XM_005601347.3 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52017	XM_005601348.3 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52018	XM_023621872.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb52019	XM_014732206.2 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb52020	XM_023621873.1 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb52020	XM_014732209.2 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb52021	XM_005601350.3 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHb08031	XM_005601347.2 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OHb08032	XM_005601348.2 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OHb08034	XM_005601350.2 Latest version!	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

MCF2L ( XM_014732205.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587691.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb36104
Clone ID Related Accession (Same CDS sequence)	XM_014732205.1
Accession Version	XM_014732205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GGGGAGCGTC CCGGGGCCGC CAGCGCTGGC TCTGCCTGCT GCCCTTGGGA 
TGCCGGGGGG CCTCGAAGCG CCCACTTTCA GATGAAATCA TGCACCAGGA CATCACCCCG 
CTCTGCGCCG CCGACATCCA GGACCAGCTG AGGAAGCGCT TTGCCTATCT GTCCGGCGGG 
CGGGGGCAGG ACGGCAGCCC CGTCATCACC TTCCCGGACT ACCCGGCCTT CAGCGACGTC 
CCAGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG CTTACAAGAT 
GCTGGCATTG GGTTCATCCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCGGTGAAG 
GCATCAGTCC TGCGAATAGC AGCGTCCTTC CCGGCAAACC TCCAGCGCGT CCTTGTCCTG 
CGCCCAACGG GATTTTTCCA GAGGACCCTC TCTGACCTCG CTTTCAGATT CAACAGAGAT 
GACTTTAAGA TGAAGGTACC GGTCACAATG CTGAGCTCAG TACCAGAATT GCACGATTAC 
ATTGACAAGT CCCAGCTGAC GGAGGACCTG GGCGGGACCC TGGATTACTG CCACACCAAG 
TGGTTGTGTC ACCGCACAGC CATTGAGAGT TTTGCTGTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC AGAGCTGGCC GAGACGGAAC TGCCCAACGA CGTCCCATCC 
ACAAGCTCTG TGCTCATCGC ACACACGGAG AAGAAGCACA AAGCAAAGGA GAACATGAGG 
CTGGCGCTGG ACGAGGGGCG GAGCATCCTG GAGAGCATCA GGGAGCCTCT GGCCAGGAGC 
ACGGAGCAGA GCCCGAACCA GGACCAGCTG GACAATCAGA CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAAACCGA GGCCGCCTTT GATGAGTTTT GGGCAAAGCA TCAGCAAAAA 
CTCGAACAGT GTCTGCAGCT CCGGCATTTT GAGCAGGATT TCCGAGAGGT CAAAGCGGCC 
TTGGACACGT TGGCCCAGAA GATAGTGACC TTCACCGACG TGGGGAACAG CCTGGCGCAC 
GTGGAGCACC TCCTGAAGGA TCTCACGAGC TTCGAGGAGA AGTCCAGTGC AGCCGTGGAG 
CGGGCCCGCG CCTTGTCCCT GGAGGGTGAG CAGCTCATCG GCAACAAGCA CTATGCGGTG 
GACTCCATCC GCCCCAAGTG CCACGAGCTG CAGCACCTCT GCGACCAGTT TGCCGCCGAG 
GTCGGGCGGA GGAGGGGGCT GCTCGGCAAG TCGCTGGAGC TGCACAGCCT CCTGGAGGCG 
TCCATGAAGT GGTGCGATGA AGGCATCTAC CTGCTGGCCT CCCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCAGA GGCCGCCCTC CAGGAGATCG AGAAGTTTCT GGAGACCGGC 
GCAGAAAATA AGATCCAGGA GCTCAATAAA ATTTACCAGG ACTACGAACT CATCCTCACC 
CAAGACCTGA GGGAACACGT GCAGAAGGTC TTCCAGAAGC AAGAGAGCAT GGAAGAGATG 
TTCCACCGGA GGCAGGCGAG CCTGAAGAAG CTGGCGGCCA AGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCCGGCCCGA GGCGCTCGCC AAGTCGCCCT GCGCCTCCCC AGGTATCCGG 
AGAGGTTCTG AGAACAGCAG TTGCGAGGGC AGCGCGCAGA GAGGGCCCTA CAGGAGGGCC 
AAGAGTGAAA TGAGTGAGGG CCGGCAGGGC CGAGGCAGCT CCACGGGGGA CGAGGAGGAG 
AGCCTGGCCA TCCTGCGGAG GCACGTCATG AACGAGCTCC TTGACACGGA ACGGGTCTAC 
GTAGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCTGCCG AGATGGACAA CCCGTTAATG 
ACACATCTCA TCTCAACAGG CCTTCAAAAC AAGAAGGATG TTCTATTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGAATATTC CTGAGGGAGC TAGAAAACTA CATAGACTGC 
CCAGAGCTGG TCGGAAGGTG TTTTCTAGAA AGGCTGGAGG AGTTCCAGAT GTACGAGAAG 
TACTGTCAGA ACAAGCCGCG TTCCGAGAGC GTGTGGCGGC AGTGCGCCGA CTGCCCCTTC 
TTCCAGGAAT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAAG 
AGCTGTGAGG GGGCCGAGGA CTTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCC ATCACGGGCT ATGACGGGAA TCTGAGCGAC 
CTGGGGAAGC TGCTCATGCA GGGCTCCTTC AGCGTCTGGA CGGACCACAA GAAGGGCCAC 
AGCAAGGTGA AGGACCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCGGTGC TCTTCTGCAA GAAGAGAGAG GAGAATGGGG AGGGCTATGA GAAAGCGCCT 
TCCTACAGCT ACAAGCAGTC CCTGAACATG GCGGCGGTTG GCATAACAGA GAACGTGAAG 
GGCGACGTGA GGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTTTA CATCATACAG 
GCACCAACTC CCGAAATCAA AGCGGCGTGG GTGAGCGAAA TCCGGAAGCT GCTGACCAGC 
CAGCTGCAGG CCTTCAGAGA AGCCAGCCAG CACCGCGTAC TGGAGCAGTC CCAGAGCCTG 
CCTCTGCCTG CAACGGCCAG CGCAAGTCCC TCGAAAGGGA ACACAAGAAA CGTCAAAAAG 
CTGGAAGAAA GGAAGACCGA CCCCCTCAGC CTGGAAGGAT ACGTGAGCTC GACATCGCTG 
CCAAAGGCCC CCGAGAAGGG CAGAGATGAC ACGGTCACTA ACTCTACCTC AGAAAGCTCT 
GCACTTTCCA AAAAGCGCTT TACCCTGCAG GGTTTTGCTA ACCTCAAAGG TCAGAAAGCT 
TCTCCCACCA GTCCTGACAA AAAAGCTAAA CGGCACGAAG TAAAGAGCGA CCCCACGCCT 
TTTGGTTTAC GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC AGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACATGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587691.1
CDS	491..4048
Translation	MAQRGASRGRQRWLCLLPLGCRGASKRPLSDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732205.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732205.1

ORF Insert Sequence:

ATGGCCCAGA GGGGAGCGTC CCGGGGCCGC CAGCGCTGGC TCTGCCTGCT GCCCTTGGGA 
TGCCGGGGGG CCTCGAAGCG CCCACTTTCA GATGAAATCA TGCACCAGGA CATCACCCCG 
CTCTGCGCCG CCGACATCCA GGACCAGCTG AGGAAGCGCT TTGCCTATCT GTCCGGCGGG 
CGGGGGCAGG ACGGCAGCCC CGTCATCACC TTCCCGGACT ACCCGGCCTT CAGCGACGTC 
CCAGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG CTTACAAGAT 
GCTGGCATTG GGTTCATCCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCGGTGAAG 
GCATCAGTCC TGCGAATAGC AGCGTCCTTC CCGGCAAACC TCCAGCGCGT CCTTGTCCTG 
CGCCCAACGG GATTTTTCCA GAGGACCCTC TCTGACCTCG CTTTCAGATT CAACAGAGAT 
GACTTTAAGA TGAAGGTACC GGTCACAATG CTGAGCTCAG TACCAGAATT GCACGATTAC 
ATTGACAAGT CCCAGCTGAC GGAGGACCTG GGCGGGACCC TGGATTACTG CCACACCAAG 
TGGTTGTGTC ACCGCACAGC CATTGAGAGT TTTGCTGTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC AGAGCTGGCC GAGACGGAAC TGCCCAACGA CGTCCCATCC 
ACAAGCTCTG TGCTCATCGC ACACACGGAG AAGAAGCACA AAGCAAAGGA GAACATGAGG 
CTGGCGCTGG ACGAGGGGCG GAGCATCCTG GAGAGCATCA GGGAGCCTCT GGCCAGGAGC 
ACGGAGCAGA GCCCGAACCA GGACCAGCTG GACAATCAGA CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAAACCGA GGCCGCCTTT GATGAGTTTT GGGCAAAGCA TCAGCAAAAA 
CTCGAACAGT GTCTGCAGCT CCGGCATTTT GAGCAGGATT TCCGAGAGGT CAAAGCGGCC 
TTGGACACGT TGGCCCAGAA GATAGTGACC TTCACCGACG TGGGGAACAG CCTGGCGCAC 
GTGGAGCACC TCCTGAAGGA TCTCACGAGC TTCGAGGAGA AGTCCAGTGC AGCCGTGGAG 
CGGGCCCGCG CCTTGTCCCT GGAGGGTGAG CAGCTCATCG GCAACAAGCA CTATGCGGTG 
GACTCCATCC GCCCCAAGTG CCACGAGCTG CAGCACCTCT GCGACCAGTT TGCCGCCGAG 
GTCGGGCGGA GGAGGGGGCT GCTCGGCAAG TCGCTGGAGC TGCACAGCCT CCTGGAGGCG 
TCCATGAAGT GGTGCGATGA AGGCATCTAC CTGCTGGCCT CCCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCAGA GGCCGCCCTC CAGGAGATCG AGAAGTTTCT GGAGACCGGC 
GCAGAAAATA AGATCCAGGA GCTCAATAAA ATTTACCAGG ACTACGAACT CATCCTCACC 
CAAGACCTGA GGGAACACGT GCAGAAGGTC TTCCAGAAGC AAGAGAGCAT GGAAGAGATG 
TTCCACCGGA GGCAGGCGAG CCTGAAGAAG CTGGCGGCCA AGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCCGGCCCGA GGCGCTCGCC AAGTCGCCCT GCGCCTCCCC AGGTATCCGG 
AGAGGTTCTG AGAACAGCAG TTGCGAGGGC AGCGCGCAGA GAGGGCCCTA CAGGAGGGCC 
AAGAGTGAAA TGAGTGAGGG CCGGCAGGGC CGAGGCAGCT CCACGGGGGA CGAGGAGGAG 
AGCCTGGCCA TCCTGCGGAG GCACGTCATG AACGAGCTCC TTGACACGGA ACGGGTCTAC 
GTAGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCTGCCG AGATGGACAA CCCGTTAATG 
ACACATCTCA TCTCAACAGG CCTTCAAAAC AAGAAGGATG TTCTATTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGAATATTC CTGAGGGAGC TAGAAAACTA CATAGACTGC 
CCAGAGCTGG TCGGAAGGTG TTTTCTAGAA AGGCTGGAGG AGTTCCAGAT GTACGAGAAG 
TACTGTCAGA ACAAGCCGCG TTCCGAGAGC GTGTGGCGGC AGTGCGCCGA CTGCCCCTTC 
TTCCAGGAAT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAAG 
AGCTGTGAGG GGGCCGAGGA CTTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCC ATCACGGGCT ATGACGGGAA TCTGAGCGAC 
CTGGGGAAGC TGCTCATGCA GGGCTCCTTC AGCGTCTGGA CGGACCACAA GAAGGGCCAC 
AGCAAGGTGA AGGACCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCGGTGC TCTTCTGCAA GAAGAGAGAG GAGAATGGGG AGGGCTATGA GAAAGCGCCT 
TCCTACAGCT ACAAGCAGTC CCTGAACATG GCGGCGGTTG GCATAACAGA GAACGTGAAG 
GGCGACGTGA GGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTTTA CATCATACAG 
GCACCAACTC CCGAAATCAA AGCGGCGTGG GTGAGCGAAA TCCGGAAGCT GCTGACCAGC 
CAGCTGCAGG CCTTCAGAGA AGCCAGCCAG CACCGCGTAC TGGAGCAGTC CCAGAGCCTG 
CCTCTGCCTG CAACGGCCAG CGCAAGTCCC TCGAAAGGGA ACACAAGAAA CGTCAAAAAG 
CTGGAAGAAA GGAAGACCGA CCCCCTCAGC CTGGAAGGAT ACGTGAGCTC GACATCGCTG 
CCAAAGGCCC CCGAGAAGGG CAGAGATGAC ACGGTCACTA ACTCTACCTC AGAAAGCTCT 
GCACTTTCCA AAAAGCGCTT TACCCTGCAG GGTTTTGCTA ACCTCAAAGG TCAGAAAGCT 
TCTCCCACCA GTCCTGACAA AAAAGCTAAA CGGCACGAAG TAAAGAGCGA CCCCACGCCT 
TTTGGTTTAC GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC AGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACATGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_014732206.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587692.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHb36105
Clone ID Related Accession (Same CDS sequence)	XM_014732206.1
Accession Version	XM_014732206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCAC TTGTTCTGCA CCTCTCAGAT GAAATCATGC ACCAGGACAT CACCCCGCTC 
TGCGCCGCCG ACATCCAGGA CCAGCTGAGG AAGCGCTTTG CCTATCTGTC CGGCGGGCGG 
GGGCAGGACG GCAGCCCCGT CATCACCTTC CCGGACTACC CGGCCTTCAG CGACGTCCCA 
GACAAGGAGT TCCAGAATGT CATGACCTAT CTCACCAGCA TCCCCAGCTT ACAAGATGCT 
GGCATTGGGT TCATCCTGGT CATAGACCGA AGGCAGGACA AATGGACCTC GGTGAAGGCA 
TCAGTCCTGC GAATAGCAGC GTCCTTCCCG GCAAACCTCC AGCGCGTCCT TGTCCTGCGC 
CCAACGGGAT TTTTCCAGAG GACCCTCTCT GACCTCGCTT TCAGATTCAA CAGAGATGAC 
TTTAAGATGA AGGTACCGGT CACAATGCTG AGCTCAGTAC CAGAATTGCA CGATTACATT 
GACAAGTCCC AGCTGACGGA GGACCTGGGC GGGACCCTGG ATTACTGCCA CACCAAGTGG 
TTGTGTCACC GCACAGCCAT TGAGAGTTTT GCTGTCATGG TGAAGCAGAC GGCTCAGATG 
CTGCAGTCCT TCGGGACAGA GCTGGCCGAG ACGGAACTGC CCAACGACGT CCCATCCACA 
AGCTCTGTGC TCATCGCACA CACGGAGAAG AAGCACAAAG CAAAGGAGAA CATGAGGCTG 
GCGCTGGACG AGGGGCGGAG CATCCTGGAG AGCATCAGGG AGCCTCTGGC CAGGAGCACG 
GAGCAGAGCC CGAACCAGGA CCAGCTGGAC AATCAGACCA CCGTGCAGAG GCTCCTGGCC 
CAGCTGAATG AAACCGAGGC CGCCTTTGAT GAGTTTTGGG CAAAGCATCA GCAAAAACTC 
GAACAGTGTC TGCAGCTCCG GCATTTTGAG CAGGATTTCC GAGAGGTCAA AGCGGCCTTG 
GACACGTTGG CCCAGAAGAT AGTGACCTTC ACCGACGTGG GGAACAGCCT GGCGCACGTG 
GAGCACCTCC TGAAGGATCT CACGAGCTTC GAGGAGAAGT CCAGTGCAGC CGTGGAGCGG 
GCCCGCGCCT TGTCCCTGGA GGGTGAGCAG CTCATCGGCA ACAAGCACTA TGCGGTGGAC 
TCCATCCGCC CCAAGTGCCA CGAGCTGCAG CACCTCTGCG ACCAGTTTGC CGCCGAGGTC 
GGGCGGAGGA GGGGGCTGCT CGGCAAGTCG CTGGAGCTGC ACAGCCTCCT GGAGGCGTCC 
ATGAAGTGGT GCGATGAAGG CATCTACCTG CTGGCCTCCC AGCCTGTGGA CAAGTGCCAG 
TCCCAGGACG GCGCAGAGGC CGCCCTCCAG GAGATCGAGA AGTTTCTGGA GACCGGCGCA 
GAAAATAAGA TCCAGGAGCT CAATAAAATT TACCAGGACT ACGAACTCAT CCTCACCCAA 
GACCTGAGGG AACACGTGCA GAAGGTCTTC CAGAAGCAAG AGAGCATGGA AGAGATGTTC 
CACCGGAGGC AGGCGAGCCT GAAGAAGCTG GCGGCCAAGC AGACGCGGCC CGTGCAGCCG 
GTGGCCCCCC GGCCCGAGGC GCTCGCCAAG TCGCCCTGCG CCTCCCCAGG TATCCGGAGA 
GGTTCTGAGA ACAGCAGTTG CGAGGGCAGC GCGCAGAGAG GGCCCTACAG GAGGGCCAAG 
AGTGAAATGA GTGAGGGCCG GCAGGGCCGA GGCAGCTCCA CGGGGGACGA GGAGGAGAGC 
CTGGCCATCC TGCGGAGGCA CGTCATGAAC GAGCTCCTTG ACACGGAACG GGTCTACGTA 
GAGGAGCTGC TGTGCGTCCT GGAGGGCTAC GCTGCCGAGA TGGACAACCC GTTAATGACA 
CATCTCATCT CAACAGGCCT TCAAAACAAG AAGGATGTTC TATTTGGAAA CATGGAAGAA 
ATTTACCACT TTCATAACAG AATATTCCTG AGGGAGCTAG AAAACTACAT AGACTGCCCA 
GAGCTGGTCG GAAGGTGTTT TCTAGAAAGG CTGGAGGAGT TCCAGATGTA CGAGAAGTAC 
TGTCAGAACA AGCCGCGTTC CGAGAGCGTG TGGCGGCAGT GCGCCGACTG CCCCTTCTTC 
CAGGAATGCC AGAAGAAGCT GGACCACAAG CTGAGCCTGG ACTCCTACCT GCTGAAGCCA 
GTCCAGAGGA TCACCAAGTA CCAGCTGCTG CTCAAGGAAA TGCTGAAATA CAGCAAGAGC 
TGTGAGGGGG CCGAGGACTT GCAGGAGGCG CTGAGCTCCA TCCTGGGCAT CCTCAAGGCC 
GTGAACGACT CCATGCACCT CATCGCCATC ACGGGCTATG ACGGGAATCT GAGCGACCTG 
GGGAAGCTGC TCATGCAGGG CTCCTTCAGC GTCTGGACGG ACCACAAGAA GGGCCACAGC 
AAGGTGAAGG ACCTGGCCAG GTTCAAGCCC ATGCAGCGGC ACCTGTTCCT GCACGAGAAG 
GCGGTGCTCT TCTGCAAGAA GAGAGAGGAG AATGGGGAGG GCTATGAGAA AGCGCCTTCC 
TACAGCTACA AGCAGTCCCT GAACATGGCG GCGGTTGGCA TAACAGAGAA CGTGAAGGGC 
GACGTGAGGA AGTTTGAGAT CTGGTACAAC GCCCGGGAGG AGGTTTACAT CATACAGGCA 
CCAACTCCCG AAATCAAAGC GGCGTGGGTG AGCGAAATCC GGAAGCTGCT GACCAGCCAG 
CTGCAGGCCT TCAGAGAAGC CAGCCAGCAC CGCGTACTGG AGCAGTCCCA GAGCCTGCCT 
CTGCCTGCAA CGGCCAGCGC AAGTCCCTCG AAAGGGAACA CAAGAAACGT CAAAAAGCTG 
GAAGAAAGGA AGACCGACCC CCTCAGCCTG GAAGGATACG TGAGCTCGAC ATCGCTGCCA 
AAGGCCCCCG AGAAGGGCAG AGATGACACG GTCACTAACT CTACCTCAGA AAGCTCTGCA 
CTTTCCAAAA AGCGCTTTAC CCTGCAGGGT TTTGCTAACC TCAAAGGTCA GAAAGCTTCT 
CCCACCAGTC CTGACAAAAA AGCTAAACGG CACGAAGTAA AGAGCGACCC CACGCCTTTT 
GGTTTACGAG GTTGGAGCAA AACGTCTCAG CCCTCCGAGG CCCCTGAGGA CAACGACGGC 
TGGTCGAGTG CTGAGGAGCC CCTTAATTCC TCGGACGCAG AGGAAGAAGG CGGAGTGGGC 
CCCAGGAAGC TGGTCCCAGG GAGGTTCACA GTTGTGGCGG ATGACGAGAA GGGCAGTCCT 
GACATGCTGG TCATGAGGAG CGGGGACCTG GTGGAGGTGG TCCAGGAGGG CGAGGACTGC 
CTCTGGTATG TCAGGAACCT GACCTCCAGC AAGGAGGGCT GGGTGCCGGC CGGCAGCCTG 
TCCGCGCGCC TCGGCAAGTC CAGCTCAGCT CAGTGCTTGA GCAGCTCAGA GTCCAGCGCA 
GGGTCCACCC TGCTGAGCAG CTCCTCCAGC TGCAGTGAGA GCTGCGGGGT CCCCCTCTCA 
GACCTGCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587692.1
CDS	130..3624
Translation	MRSLVLHLSDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732206.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732206.1

ORF Insert Sequence:

ATGAGGTCAC TTGTTCTGCA CCTCTCAGAT GAAATCATGC ACCAGGACAT CACCCCGCTC 
TGCGCCGCCG ACATCCAGGA CCAGCTGAGG AAGCGCTTTG CCTATCTGTC CGGCGGGCGG 
GGGCAGGACG GCAGCCCCGT CATCACCTTC CCGGACTACC CGGCCTTCAG CGACGTCCCA 
GACAAGGAGT TCCAGAATGT CATGACCTAT CTCACCAGCA TCCCCAGCTT ACAAGATGCT 
GGCATTGGGT TCATCCTGGT CATAGACCGA AGGCAGGACA AATGGACCTC GGTGAAGGCA 
TCAGTCCTGC GAATAGCAGC GTCCTTCCCG GCAAACCTCC AGCGCGTCCT TGTCCTGCGC 
CCAACGGGAT TTTTCCAGAG GACCCTCTCT GACCTCGCTT TCAGATTCAA CAGAGATGAC 
TTTAAGATGA AGGTACCGGT CACAATGCTG AGCTCAGTAC CAGAATTGCA CGATTACATT 
GACAAGTCCC AGCTGACGGA GGACCTGGGC GGGACCCTGG ATTACTGCCA CACCAAGTGG 
TTGTGTCACC GCACAGCCAT TGAGAGTTTT GCTGTCATGG TGAAGCAGAC GGCTCAGATG 
CTGCAGTCCT TCGGGACAGA GCTGGCCGAG ACGGAACTGC CCAACGACGT CCCATCCACA 
AGCTCTGTGC TCATCGCACA CACGGAGAAG AAGCACAAAG CAAAGGAGAA CATGAGGCTG 
GCGCTGGACG AGGGGCGGAG CATCCTGGAG AGCATCAGGG AGCCTCTGGC CAGGAGCACG 
GAGCAGAGCC CGAACCAGGA CCAGCTGGAC AATCAGACCA CCGTGCAGAG GCTCCTGGCC 
CAGCTGAATG AAACCGAGGC CGCCTTTGAT GAGTTTTGGG CAAAGCATCA GCAAAAACTC 
GAACAGTGTC TGCAGCTCCG GCATTTTGAG CAGGATTTCC GAGAGGTCAA AGCGGCCTTG 
GACACGTTGG CCCAGAAGAT AGTGACCTTC ACCGACGTGG GGAACAGCCT GGCGCACGTG 
GAGCACCTCC TGAAGGATCT CACGAGCTTC GAGGAGAAGT CCAGTGCAGC CGTGGAGCGG 
GCCCGCGCCT TGTCCCTGGA GGGTGAGCAG CTCATCGGCA ACAAGCACTA TGCGGTGGAC 
TCCATCCGCC CCAAGTGCCA CGAGCTGCAG CACCTCTGCG ACCAGTTTGC CGCCGAGGTC 
GGGCGGAGGA GGGGGCTGCT CGGCAAGTCG CTGGAGCTGC ACAGCCTCCT GGAGGCGTCC 
ATGAAGTGGT GCGATGAAGG CATCTACCTG CTGGCCTCCC AGCCTGTGGA CAAGTGCCAG 
TCCCAGGACG GCGCAGAGGC CGCCCTCCAG GAGATCGAGA AGTTTCTGGA GACCGGCGCA 
GAAAATAAGA TCCAGGAGCT CAATAAAATT TACCAGGACT ACGAACTCAT CCTCACCCAA 
GACCTGAGGG AACACGTGCA GAAGGTCTTC CAGAAGCAAG AGAGCATGGA AGAGATGTTC 
CACCGGAGGC AGGCGAGCCT GAAGAAGCTG GCGGCCAAGC AGACGCGGCC CGTGCAGCCG 
GTGGCCCCCC GGCCCGAGGC GCTCGCCAAG TCGCCCTGCG CCTCCCCAGG TATCCGGAGA 
GGTTCTGAGA ACAGCAGTTG CGAGGGCAGC GCGCAGAGAG GGCCCTACAG GAGGGCCAAG 
AGTGAAATGA GTGAGGGCCG GCAGGGCCGA GGCAGCTCCA CGGGGGACGA GGAGGAGAGC 
CTGGCCATCC TGCGGAGGCA CGTCATGAAC GAGCTCCTTG ACACGGAACG GGTCTACGTA 
GAGGAGCTGC TGTGCGTCCT GGAGGGCTAC GCTGCCGAGA TGGACAACCC GTTAATGACA 
CATCTCATCT CAACAGGCCT TCAAAACAAG AAGGATGTTC TATTTGGAAA CATGGAAGAA 
ATTTACCACT TTCATAACAG AATATTCCTG AGGGAGCTAG AAAACTACAT AGACTGCCCA 
GAGCTGGTCG GAAGGTGTTT TCTAGAAAGG CTGGAGGAGT TCCAGATGTA CGAGAAGTAC 
TGTCAGAACA AGCCGCGTTC CGAGAGCGTG TGGCGGCAGT GCGCCGACTG CCCCTTCTTC 
CAGGAATGCC AGAAGAAGCT GGACCACAAG CTGAGCCTGG ACTCCTACCT GCTGAAGCCA 
GTCCAGAGGA TCACCAAGTA CCAGCTGCTG CTCAAGGAAA TGCTGAAATA CAGCAAGAGC 
TGTGAGGGGG CCGAGGACTT GCAGGAGGCG CTGAGCTCCA TCCTGGGCAT CCTCAAGGCC 
GTGAACGACT CCATGCACCT CATCGCCATC ACGGGCTATG ACGGGAATCT GAGCGACCTG 
GGGAAGCTGC TCATGCAGGG CTCCTTCAGC GTCTGGACGG ACCACAAGAA GGGCCACAGC 
AAGGTGAAGG ACCTGGCCAG GTTCAAGCCC ATGCAGCGGC ACCTGTTCCT GCACGAGAAG 
GCGGTGCTCT TCTGCAAGAA GAGAGAGGAG AATGGGGAGG GCTATGAGAA AGCGCCTTCC 
TACAGCTACA AGCAGTCCCT GAACATGGCG GCGGTTGGCA TAACAGAGAA CGTGAAGGGC 
GACGTGAGGA AGTTTGAGAT CTGGTACAAC GCCCGGGAGG AGGTTTACAT CATACAGGCA 
CCAACTCCCG AAATCAAAGC GGCGTGGGTG AGCGAAATCC GGAAGCTGCT GACCAGCCAG 
CTGCAGGCCT TCAGAGAAGC CAGCCAGCAC CGCGTACTGG AGCAGTCCCA GAGCCTGCCT 
CTGCCTGCAA CGGCCAGCGC AAGTCCCTCG AAAGGGAACA CAAGAAACGT CAAAAAGCTG 
GAAGAAAGGA AGACCGACCC CCTCAGCCTG GAAGGATACG TGAGCTCGAC ATCGCTGCCA 
AAGGCCCCCG AGAAGGGCAG AGATGACACG GTCACTAACT CTACCTCAGA AAGCTCTGCA 
CTTTCCAAAA AGCGCTTTAC CCTGCAGGGT TTTGCTAACC TCAAAGGTCA GAAAGCTTCT 
CCCACCAGTC CTGACAAAAA AGCTAAACGG CACGAAGTAA AGAGCGACCC CACGCCTTTT 
GGTTTACGAG GTTGGAGCAA AACGTCTCAG CCCTCCGAGG CCCCTGAGGA CAACGACGGC 
TGGTCGAGTG CTGAGGAGCC CCTTAATTCC TCGGACGCAG AGGAAGAAGG CGGAGTGGGC 
CCCAGGAAGC TGGTCCCAGG GAGGTTCACA GTTGTGGCGG ATGACGAGAA GGGCAGTCCT 
GACATGCTGG TCATGAGGAG CGGGGACCTG GTGGAGGTGG TCCAGGAGGG CGAGGACTGC 
CTCTGGTATG TCAGGAACCT GACCTCCAGC AAGGAGGGCT GGGTGCCGGC CGGCAGCCTG 
TCCGCGCGCC TCGGCAAGTC CAGCTCAGCT CAGTGCTTGA GCAGCTCAGA GTCCAGCGCA 
GGGTCCACCC TGCTGAGCAG CTCCTCCAGC TGCAGTGAGA GCTGCGGGGT CCCCCTCTCA 
GACCTGCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_014732207.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587693.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.

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CloneID	OHb36106
Clone ID Related Accession (Same CDS sequence)	XM_014732207.1 , XM_014732208.1 , XM_014732209.1
Accession Version	XM_014732207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCGGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCCGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAATGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CAGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACATG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587693.1
CDS	96..3554
Translation	MHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732207.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732207.1

ORF Insert Sequence:

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCGGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCCGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAATGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CAGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACATG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_014732208.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587694.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.

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CloneID	OHb36106
Clone ID Related Accession (Same CDS sequence)	XM_014732207.1 , XM_014732208.1 , XM_014732209.1
Accession Version	XM_014732208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCGGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCCGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAATGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CAGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACATG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587694.1
CDS	291..3749
Translation	MHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732208.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732208.1

ORF Insert Sequence:

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCGGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCCGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAATGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CAGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACATG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_014732209.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587695.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHb36106
Clone ID Related Accession (Same CDS sequence)	XM_014732207.1 , XM_014732208.1 , XM_014732209.1
Accession Version	XM_014732209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCGGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCCGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAATGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CAGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACATG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587695.1
CDS	161..3619
Translation	MHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732209.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732209.1

ORF Insert Sequence:

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCGGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCCGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAATGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CAGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACATG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621862.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477630.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.

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CloneID	OHb52006
Clone ID Related Accession (Same CDS sequence)	XM_023621862.1
Accession Version	XM_023621862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGGTCAGA AAGCTTCTCC CACCAGTCCT GACAAAAAAG CTAAACGGCA CGAAGTAAAG 
AGCGACCCCA CGCCTTTTGG TTTACGAGGT TGGAGCAAAA CGTCTCAGCC CTCCGAGGCC 
CCTGAGGACA ACGACGGCTG GTCGAGTGCT GAGGAGCCCC TTAATTCCTC GGACGCAGAG 
GAAGAAGGCG GAGTGGGCCC CAGGAAGCTG GTCCCGGGGA GGTTCACAGT TGTGGCGGAT 
GACGAGAAGG GCAGTCCTGA CACGCTGGTC ATGAGGAGCG GGGACCTGGT GGAGGTGGTC 
CAGGAGGGCG AGGACTGCCT CTGGTATGTC AGGAACCTGA CCTCCAGCAA GGAGGGCTGG 
GTGCCGGCCG GCAGCCTGTC CGCGCGCCTC GGCAAGTCCA GCTCAGCTCA GTGCTTGAGC 
AGCTCAGAGT CCAGCGCAGG GTCCACCCTG CTGAGCAGCT CCTCCAGCTG CAGTGAGAGC 
TGCGGGGTCC CCCTCTCAGA CCTGCAGGGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477630.1
CDS	16..3708
Translation	MVQCPRPPPGQAGPTLGRLMFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621862.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621862.1

ORF Insert Sequence:

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGGTCAGA AAGCTTCTCC CACCAGTCCT GACAAAAAAG CTAAACGGCA CGAAGTAAAG 
AGCGACCCCA CGCCTTTTGG TTTACGAGGT TGGAGCAAAA CGTCTCAGCC CTCCGAGGCC 
CCTGAGGACA ACGACGGCTG GTCGAGTGCT GAGGAGCCCC TTAATTCCTC GGACGCAGAG 
GAAGAAGGCG GAGTGGGCCC CAGGAAGCTG GTCCCGGGGA GGTTCACAGT TGTGGCGGAT 
GACGAGAAGG GCAGTCCTGA CACGCTGGTC ATGAGGAGCG GGGACCTGGT GGAGGTGGTC 
CAGGAGGGCG AGGACTGCCT CTGGTATGTC AGGAACCTGA CCTCCAGCAA GGAGGGCTGG 
GTGCCGGCCG GCAGCCTGTC CGCGCGCCTC GGCAAGTCCA GCTCAGCTCA GTGCTTGAGC 
AGCTCAGAGT CCAGCGCAGG GTCCACCCTG CTGAGCAGCT CCTCCAGCTG CAGTGAGAGC 
TGCGGGGTCC CCCTCTCAGA CCTGCAGGGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621863.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477631.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb52007
Clone ID Related Accession (Same CDS sequence)	XM_023621863.1
Accession Version	XM_023621863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGCTTCTC CCACCAGTCC TGACAAAAAA GCTAAACGGC ACGAAGTAAA GAGCGACCCC 
ACGCCTTTTG GTTTACGAGG TTGGAGCAAA ACGTCTCAGC CCTCCGAGGC CCCTGAGGAC 
AACGACGGCT GGTCGAGTGC TGAGGAGCCC CTTAATTCCT CGGACGCAGA GGAAGAAGGC 
GGAGTGGGCC CCAGGAAGCT GGTCCCGGGG AGGTTCACAG TTGTGGCGGA TGACGAGAAG 
GGCAGTCCTG ACACGCTGGT CATGAGGAGC GGGGACCTGG TGGAGGTGGT CCAGGAGGGC 
GAGGACTGCC TCTGGTATGT CAGGAACCTG ACCTCCAGCA AGGAGGGCTG GGTGCCGGCC 
GGCAGCCTGT CCGCGCGCCT CGGCAAGTCC AGCTCAGCTC AGTGCTTGAG CAGCTCAGAG 
TCCAGCGCAG GGTCCACCCT GCTGAGCAGC TCCTCCAGCT GCAGTGAGAG CTGCGGGGTC 
CCCCTCTCAG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477631.1
CDS	16..3699
Translation	MVQCPRPPPGQAGPTLGRLMFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621863.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621863.1

ORF Insert Sequence:

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGCTTCTC CCACCAGTCC TGACAAAAAA GCTAAACGGC ACGAAGTAAA GAGCGACCCC 
ACGCCTTTTG GTTTACGAGG TTGGAGCAAA ACGTCTCAGC CCTCCGAGGC CCCTGAGGAC 
AACGACGGCT GGTCGAGTGC TGAGGAGCCC CTTAATTCCT CGGACGCAGA GGAAGAAGGC 
GGAGTGGGCC CCAGGAAGCT GGTCCCGGGG AGGTTCACAG TTGTGGCGGA TGACGAGAAG 
GGCAGTCCTG ACACGCTGGT CATGAGGAGC GGGGACCTGG TGGAGGTGGT CCAGGAGGGC 
GAGGACTGCC TCTGGTATGT CAGGAACCTG ACCTCCAGCA AGGAGGGCTG GGTGCCGGCC 
GGCAGCCTGT CCGCGCGCCT CGGCAAGTCC AGCTCAGCTC AGTGCTTGAG CAGCTCAGAG 
TCCAGCGCAG GGTCCACCCT GCTGAGCAGC TCCTCCAGCT GCAGTGAGAG CTGCGGGGTC 
CCCCTCTCAG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621864.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477632.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb52008
Clone ID Related Accession (Same CDS sequence)	XM_023621864.1
Accession Version	XM_023621864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGGTCAGA AAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC GGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACACGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477632.1
CDS	16..3633
Translation	MVQCPRPPPGQAGPTLGRLMFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621864.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621864.1

ORF Insert Sequence:

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGGTCAGA AAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC GGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACACGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621865.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477633.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHb52009
Clone ID Related Accession (Same CDS sequence)	XM_023621865.1
Accession Version	XM_023621865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGGTCAGA AAGCTTCTCC CACCAGTCCT GACAAAAAAG CTAAACGGCA CGAAGTAAAG 
AGCGACCCCA CGCCTTTTGG TTTACGAGGT TGGAGCAAAA CGTCTCAGCC CTCCGAGGCC 
CCTGAGGACA ACGACGGCTG GTCGAGTGCT GAGGAGCCCC TTAATTCCTC GGACGCAGAG 
GAAGAAGGCG GAGTGGGCCC CAGGAAGCTG GTCCCGGGGA GGTTCACAGT TGTGGCGGAT 
GACGAGAAGG GCAGTCCTGA CACGCTGGTC ATGAGGAGCG GGGACCTGGT GGAGGTGGTC 
CAGGAGGGCG AGGACTGCCT CTGGTATGTC AGGAACCTGA CCTCCAGCAA GGAGGGCTGG 
GTGCCGGCCG GCAGCCTGTC CGCGCGCCTC GGCAAGTCCA GCTCAGCTCA GTGCTTGAGC 
AGCTCAGTTT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477633.1
CDS	16..3630
Translation	MVQCPRPPPGQAGPTLGRLMFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSVW

Target ORF information:

RefSeq Version	XM_023621865.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621865.1

ORF Insert Sequence:

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG ATGACACGGT CACTAACTCT 
ACCTCAGAAA GCTCTGCACT TTCCAAAAAG CGCTTTACCC TGCAGGGTTT TGCTAACCTC 
AAAGGTCAGA AAGCTTCTCC CACCAGTCCT GACAAAAAAG CTAAACGGCA CGAAGTAAAG 
AGCGACCCCA CGCCTTTTGG TTTACGAGGT TGGAGCAAAA CGTCTCAGCC CTCCGAGGCC 
CCTGAGGACA ACGACGGCTG GTCGAGTGCT GAGGAGCCCC TTAATTCCTC GGACGCAGAG 
GAAGAAGGCG GAGTGGGCCC CAGGAAGCTG GTCCCGGGGA GGTTCACAGT TGTGGCGGAT 
GACGAGAAGG GCAGTCCTGA CACGCTGGTC ATGAGGAGCG GGGACCTGGT GGAGGTGGTC 
CAGGAGGGCG AGGACTGCCT CTGGTATGTC AGGAACCTGA CCTCCAGCAA GGAGGGCTGG 
GTGCCGGCCG GCAGCCTGTC CGCGCGCCTC GGCAAGTCCA GCTCAGCTCA GTGCTTGAGC 
AGCTCAGTTT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621866.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477634.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHb52010
Clone ID Related Accession (Same CDS sequence)	XM_023621866.1
Accession Version	XM_023621866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTTG CGTCACTGTC TCATCTGAGA GTCTTCCTTT TGCTGACGGG GAGGGTCAGG 
CAGTCATGCT TGACCTGGAG GGTTGTCAGG AGCGACCGTG GCGATGGGCC GGCAGGCGCT 
GGTGGGCGGC TGGTGTGCAT CCTGGGTTTG CTGCGTGGAC CACGGCCATC CAGGCTTCCC 
GGTTCTCCTG TCACTGTCAT GGTAGCGACA GGACGCCACA GACAGGAGCC GGATGAAATC 
ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCCGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCAGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAACGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CGGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACACG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477634.1
CDS	780..4478
Translation	MSFASLSHLRVFLLLTGRVRQSCLTWRVVRSDRGDGPAGAGGRLVCILGLLRGPRPSRLPGSPVTVMVATGRHRQEPDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621866.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621866.1

ORF Insert Sequence:

ATGAGCTTTG CGTCACTGTC TCATCTGAGA GTCTTCCTTT TGCTGACGGG GAGGGTCAGG 
CAGTCATGCT TGACCTGGAG GGTTGTCAGG AGCGACCGTG GCGATGGGCC GGCAGGCGCT 
GGTGGGCGGC TGGTGTGCAT CCTGGGTTTG CTGCGTGGAC CACGGCCATC CAGGCTTCCC 
GGTTCTCCTG TCACTGTCAT GGTAGCGACA GGACGCCACA GACAGGAGCC GGATGAAATC 
ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCCGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCAGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAACGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CGGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACACG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621867.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477635.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.

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CloneID	OHb52011
Clone ID Related Accession (Same CDS sequence)	XM_023621867.1
Accession Version	XM_023621867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG CTTCTCCCAC CAGTCCTGAC 
AAAAAAGCTA AACGGCACGA AGTAAAGAGC GACCCCACGC CTTTTGGTTT ACGAGGTTGG 
AGCAAAACGT CTCAGCCCTC CGAGGCCCCT GAGGACAACG ACGGCTGGTC GAGTGCTGAG 
GAGCCCCTTA ATTCCTCGGA CGCAGAGGAA GAAGGCGGAG TGGGCCCCAG GAAGCTGGTC 
CCGGGGAGGT TCACAGTTGT GGCGGATGAC GAGAAGGGCA GTCCTGACAC GCTGGTCATG 
AGGAGCGGGG ACCTGGTGGA GGTGGTCCAG GAGGGCGAGG ACTGCCTCTG GTATGTCAGG 
AACCTGACCT CCAGCAAGGA GGGCTGGGTG CCGGCCGGCA GCCTGTCCGC GCGCCTCGGC 
AAGTCCAGCT CAGCTCAGTG CTTGAGCAGC TCAGAGTCCA GCGCAGGGTC CACCCTGCTG 
AGCAGCTCCT CCAGCTGCAG TGAGAGCTGC GGGGTCCCCC TCTCAGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477635.1
CDS	16..3615
Translation	MVQCPRPPPGQAGPTLGRLMFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621867.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621867.1

ORF Insert Sequence:

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG CTTCTCCCAC CAGTCCTGAC 
AAAAAAGCTA AACGGCACGA AGTAAAGAGC GACCCCACGC CTTTTGGTTT ACGAGGTTGG 
AGCAAAACGT CTCAGCCCTC CGAGGCCCCT GAGGACAACG ACGGCTGGTC GAGTGCTGAG 
GAGCCCCTTA ATTCCTCGGA CGCAGAGGAA GAAGGCGGAG TGGGCCCCAG GAAGCTGGTC 
CCGGGGAGGT TCACAGTTGT GGCGGATGAC GAGAAGGGCA GTCCTGACAC GCTGGTCATG 
AGGAGCGGGG ACCTGGTGGA GGTGGTCCAG GAGGGCGAGG ACTGCCTCTG GTATGTCAGG 
AACCTGACCT CCAGCAAGGA GGGCTGGGTG CCGGCCGGCA GCCTGTCCGC GCGCCTCGGC 
AAGTCCAGCT CAGCTCAGTG CTTGAGCAGC TCAGAGTCCA GCGCAGGGTC CACCCTGCTG 
AGCAGCTCCT CCAGCTGCAG TGAGAGCTGC GGGGTCCCCC TCTCAGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621869.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477637.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHb52012
Clone ID Related Accession (Same CDS sequence)	XM_023621869.1 , XM_023621868.1
Accession Version	XM_023621869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACT GCTGGAGATT CATCCTGTGC AAGAAAACCG GACGCGACGA CTGTTCTTCC 
TCGCCGCGAT CCAATGAGGC CAAAACGGAG GAGAGTGACC ATTGGATTGA TGTCCCAGAC 
GGCATCAGGC TCGTTGGAGA GCAGCGCGAG GGGATGGCCA CACCCACAGA TGAAATCATG 
CACCAGGACA TCACCCCGCT CTGCGCCGCC GACATCCAGG ACCAGCTGAG GAAGCGCTTT 
GCCTATCTGT CCGGCGGGCG GGGGCAGGAC GGCAGCCCCG TCATCACCTT CCCGGACTAC 
CCGGCCTTCA GCGACGTCCC AGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGCT TACAAGATGC TGGCATTGGG TTCATCCTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CGGTGAAGGC ATCAGTCCTG CGAATAGCAG CGTCCTTCCC GGCAAACCTC 
CAGCGCGTCC TTGTCCTGCG CCCAACGGGA TTTTTCCAGA GGACCCTCTC TGACCTCGCT 
TTCAGATTCA ACAGAGATGA CTTTAAGATG AAGGTACCGG TCACAATGCT GAGCTCAGTA 
CCAGAATTGC ACGATTACAT TGACAAGTCC CAGCTGACGG AGGACCTGGG CGGGACCCTG 
GATTACTGCC ACACCAAGTG GTTGTGTCAC CGCACAGCCA TTGAGAGTTT TGCTGTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACAG AGCTGGCCGA GACGGAACTG 
CCCAACGACG TCCCATCCAC AAGCTCTGTG CTCATCGCAC ACACGGAGAA GAAGCACAAA 
GCAAAGGAGA ACATGAGGCT GGCGCTGGAC GAGGGGCGGA GCATCCTGGA GAGCATCAGG 
GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC CCGAACCAGG ACCAGCTGGA CAATCAGACC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAAACCGAGG CCGCCTTTGA TGAGTTTTGG 
GCAAAGCATC AGCAAAAACT CGAACAGTGT CTGCAGCTCC GGCATTTTGA GCAGGATTTC 
CGAGAGGTCA AAGCCGCCTT GGACACGTTG GCCCAGAAGA TAGTGACCTT CACCGACGTG 
GGGAACAGCC TGGCGCACGT GGAGCACCTC CTGAAGGATC TCACGAGCTT CGAGGAGAAG 
TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC TTGTCCCTGG AGGGTGAGCA GCTCATCGGC 
AACAAGCACT ATGCGGTGGA CTCCATCCGC CCCAAGTGCC ACGAGCTGCA GCACCTCTGC 
GACCAGTTTG CCGCCGAGGT CGGGCGGAGG AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG 
CACAGCCTCC TGGAGGCGTC CATGAAGTGG TGCGATGAAG GCATCTACCT GCTGGCCTCC 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCAGAGG CAGCCCTCCA GGAGATCGAG 
AAGTTTCTGG AGACCGGCGC AGAAAATAAG ATCCAGGAGC TCAATAAAAT TTACCAGGAC 
TACGAACTCA TCCTCACCCA AGACCTGAGG GAACACGTGC AGAAGGTCTT CCAGAAGCAA 
GAGAGCATGG AAGAGATGTT CCACCGGAGG CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC 
GCCTCCCCAG GTATCCGGAG AGGTTCTGAG AACAGCAGTT GCGAGGGCAG CGCGCAGAGA 
GGGCCCTACA GGAGGGCCAA GAGTGAAATG AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC 
ACGGGGGACG AGGAGGAGAG CCTGGCCATC CTGCGGAGGC ACGTCATGAA CGAGCTCCTT 
GACACGGAAC GGGTCTACGT AGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG 
ATGGACAACC CGTTAATGAC ACATCTCATC TCAACAGGCC TTCAAAACAA GAAGGATGTT 
CTATTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GAATATTCCT GAGGGAGCTA 
GAAAACTACA TAGACTGCCC AGAGCTGGTC GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG 
TTCCAGATGT ACGAGAAGTA CTGTCAGAAC AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG 
TGCGCCGACT GCCCCTTCTT CCAGGAATGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG GCCGAGGACT TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TCATCGCCAT CACGGGCTAT 
GACGGGAATC TGAGCGACCT GGGGAAGCTG CTCATGCAGG GCTCCTTCAG CGTCTGGACG 
GACCACAAGA AGGGCCACAG CAAGGTGAAG GACCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCGGTGCTC TTCTGCAAGA AGAGAGAGGA GAACGGGGAG 
GGCTATGAGA AAGCGCCTTC CTACAGCTAC AAGCAGTCCC TGAACATGGC GGCGGTTGGC 
ATAACAGAGA ACGTGAAGGG CGACGTGAGG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTTTACA TCATACAGGC ACCAACTCCC GAAATCAAAG CGGCGTGGGT GAGCGAAATC 
CGGAAGCTGC TGACCAGCCA GCTGCAGGCC TTCAGAGAAG CCAGCCAGCA CCGCGTACTG 
GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC 
ACAAGAAACG TCAAAAAGCT GGAAGAAAGG AAGACCGACC CCCTCAGCCT GGAAGGATAC 
GTGAGCTCGA CATCGCTGCC AAAGGCCCCC GAGAAGGGCA GAGATGACAC GGTCACTAAC 
TCTACCTCAG AAAGCTCTGC ACTTTCCAAA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCCACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA 
AAGAGCGACC CCACGCCTTT TGGTTTACGA GGTTGGAGCA AAACGTCTCA GCCCTCCGAG 
GCCCCTGAGG ACAACGACGG CTGGTCGAGT GCTGAGGAGC CCCTTAATTC CTCGGACGCA 
GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG CTGGTCCCGG GGAGGTTCAC AGTTGTGGCG 
GATGACGAGA AGGGCAGTCC TGACACGCTG GTCATGAGGA GCGGGGACCT GGTGGAGGTG 
GTCCAGGAGG GCGAGGACTG CCTCTGGTAT GTCAGGAACC TGACCTCCAG CAAGGAGGGC 
TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG 
AGCAGCTCAG AGTCCAGCGC AGGGTCCACC CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG 
AGCTGCGGGG TCCCCCTCTC AGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477637.1
CDS	23..3658
Translation	MFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621869.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621869.1

ORF Insert Sequence:

ATGTTTGACT GCTGGAGATT CATCCTGTGC AAGAAAACCG GACGCGACGA CTGTTCTTCC 
TCGCCGCGAT CCAATGAGGC CAAAACGGAG GAGAGTGACC ATTGGATTGA TGTCCCAGAC 
GGCATCAGGC TCGTTGGAGA GCAGCGCGAG GGGATGGCCA CACCCACAGA TGAAATCATG 
CACCAGGACA TCACCCCGCT CTGCGCCGCC GACATCCAGG ACCAGCTGAG GAAGCGCTTT 
GCCTATCTGT CCGGCGGGCG GGGGCAGGAC GGCAGCCCCG TCATCACCTT CCCGGACTAC 
CCGGCCTTCA GCGACGTCCC AGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGCT TACAAGATGC TGGCATTGGG TTCATCCTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CGGTGAAGGC ATCAGTCCTG CGAATAGCAG CGTCCTTCCC GGCAAACCTC 
CAGCGCGTCC TTGTCCTGCG CCCAACGGGA TTTTTCCAGA GGACCCTCTC TGACCTCGCT 
TTCAGATTCA ACAGAGATGA CTTTAAGATG AAGGTACCGG TCACAATGCT GAGCTCAGTA 
CCAGAATTGC ACGATTACAT TGACAAGTCC CAGCTGACGG AGGACCTGGG CGGGACCCTG 
GATTACTGCC ACACCAAGTG GTTGTGTCAC CGCACAGCCA TTGAGAGTTT TGCTGTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACAG AGCTGGCCGA GACGGAACTG 
CCCAACGACG TCCCATCCAC AAGCTCTGTG CTCATCGCAC ACACGGAGAA GAAGCACAAA 
GCAAAGGAGA ACATGAGGCT GGCGCTGGAC GAGGGGCGGA GCATCCTGGA GAGCATCAGG 
GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC CCGAACCAGG ACCAGCTGGA CAATCAGACC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAAACCGAGG CCGCCTTTGA TGAGTTTTGG 
GCAAAGCATC AGCAAAAACT CGAACAGTGT CTGCAGCTCC GGCATTTTGA GCAGGATTTC 
CGAGAGGTCA AAGCCGCCTT GGACACGTTG GCCCAGAAGA TAGTGACCTT CACCGACGTG 
GGGAACAGCC TGGCGCACGT GGAGCACCTC CTGAAGGATC TCACGAGCTT CGAGGAGAAG 
TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC TTGTCCCTGG AGGGTGAGCA GCTCATCGGC 
AACAAGCACT ATGCGGTGGA CTCCATCCGC CCCAAGTGCC ACGAGCTGCA GCACCTCTGC 
GACCAGTTTG CCGCCGAGGT CGGGCGGAGG AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG 
CACAGCCTCC TGGAGGCGTC CATGAAGTGG TGCGATGAAG GCATCTACCT GCTGGCCTCC 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCAGAGG CAGCCCTCCA GGAGATCGAG 
AAGTTTCTGG AGACCGGCGC AGAAAATAAG ATCCAGGAGC TCAATAAAAT TTACCAGGAC 
TACGAACTCA TCCTCACCCA AGACCTGAGG GAACACGTGC AGAAGGTCTT CCAGAAGCAA 
GAGAGCATGG AAGAGATGTT CCACCGGAGG CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC 
GCCTCCCCAG GTATCCGGAG AGGTTCTGAG AACAGCAGTT GCGAGGGCAG CGCGCAGAGA 
GGGCCCTACA GGAGGGCCAA GAGTGAAATG AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC 
ACGGGGGACG AGGAGGAGAG CCTGGCCATC CTGCGGAGGC ACGTCATGAA CGAGCTCCTT 
GACACGGAAC GGGTCTACGT AGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG 
ATGGACAACC CGTTAATGAC ACATCTCATC TCAACAGGCC TTCAAAACAA GAAGGATGTT 
CTATTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GAATATTCCT GAGGGAGCTA 
GAAAACTACA TAGACTGCCC AGAGCTGGTC GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG 
TTCCAGATGT ACGAGAAGTA CTGTCAGAAC AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG 
TGCGCCGACT GCCCCTTCTT CCAGGAATGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG GCCGAGGACT TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TCATCGCCAT CACGGGCTAT 
GACGGGAATC TGAGCGACCT GGGGAAGCTG CTCATGCAGG GCTCCTTCAG CGTCTGGACG 
GACCACAAGA AGGGCCACAG CAAGGTGAAG GACCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCGGTGCTC TTCTGCAAGA AGAGAGAGGA GAACGGGGAG 
GGCTATGAGA AAGCGCCTTC CTACAGCTAC AAGCAGTCCC TGAACATGGC GGCGGTTGGC 
ATAACAGAGA ACGTGAAGGG CGACGTGAGG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTTTACA TCATACAGGC ACCAACTCCC GAAATCAAAG CGGCGTGGGT GAGCGAAATC 
CGGAAGCTGC TGACCAGCCA GCTGCAGGCC TTCAGAGAAG CCAGCCAGCA CCGCGTACTG 
GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC 
ACAAGAAACG TCAAAAAGCT GGAAGAAAGG AAGACCGACC CCCTCAGCCT GGAAGGATAC 
GTGAGCTCGA CATCGCTGCC AAAGGCCCCC GAGAAGGGCA GAGATGACAC GGTCACTAAC 
TCTACCTCAG AAAGCTCTGC ACTTTCCAAA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCCACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA 
AAGAGCGACC CCACGCCTTT TGGTTTACGA GGTTGGAGCA AAACGTCTCA GCCCTCCGAG 
GCCCCTGAGG ACAACGACGG CTGGTCGAGT GCTGAGGAGC CCCTTAATTC CTCGGACGCA 
GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG CTGGTCCCGG GGAGGTTCAC AGTTGTGGCG 
GATGACGAGA AGGGCAGTCC TGACACGCTG GTCATGAGGA GCGGGGACCT GGTGGAGGTG 
GTCCAGGAGG GCGAGGACTG CCTCTGGTAT GTCAGGAACC TGACCTCCAG CAAGGAGGGC 
TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG 
AGCAGCTCAG AGTCCAGCGC AGGGTCCACC CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG 
AGCTGCGGGG TCCCCCTCTC AGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621868.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477636.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHb52012
Clone ID Related Accession (Same CDS sequence)	XM_023621869.1 , XM_023621868.1
Accession Version	XM_023621868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGACT GCTGGAGATT CATCCTGTGC AAGAAAACCG GACGCGACGA CTGTTCTTCC 
TCGCCGCGAT CCAATGAGGC CAAAACGGAG GAGAGTGACC ATTGGATTGA TGTCCCAGAC 
GGCATCAGGC TCGTTGGAGA GCAGCGCGAG GGGATGGCCA CACCCACAGA TGAAATCATG 
CACCAGGACA TCACCCCGCT CTGCGCCGCC GACATCCAGG ACCAGCTGAG GAAGCGCTTT 
GCCTATCTGT CCGGCGGGCG GGGGCAGGAC GGCAGCCCCG TCATCACCTT CCCGGACTAC 
CCGGCCTTCA GCGACGTCCC AGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGCT TACAAGATGC TGGCATTGGG TTCATCCTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CGGTGAAGGC ATCAGTCCTG CGAATAGCAG CGTCCTTCCC GGCAAACCTC 
CAGCGCGTCC TTGTCCTGCG CCCAACGGGA TTTTTCCAGA GGACCCTCTC TGACCTCGCT 
TTCAGATTCA ACAGAGATGA CTTTAAGATG AAGGTACCGG TCACAATGCT GAGCTCAGTA 
CCAGAATTGC ACGATTACAT TGACAAGTCC CAGCTGACGG AGGACCTGGG CGGGACCCTG 
GATTACTGCC ACACCAAGTG GTTGTGTCAC CGCACAGCCA TTGAGAGTTT TGCTGTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACAG AGCTGGCCGA GACGGAACTG 
CCCAACGACG TCCCATCCAC AAGCTCTGTG CTCATCGCAC ACACGGAGAA GAAGCACAAA 
GCAAAGGAGA ACATGAGGCT GGCGCTGGAC GAGGGGCGGA GCATCCTGGA GAGCATCAGG 
GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC CCGAACCAGG ACCAGCTGGA CAATCAGACC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAAACCGAGG CCGCCTTTGA TGAGTTTTGG 
GCAAAGCATC AGCAAAAACT CGAACAGTGT CTGCAGCTCC GGCATTTTGA GCAGGATTTC 
CGAGAGGTCA AAGCCGCCTT GGACACGTTG GCCCAGAAGA TAGTGACCTT CACCGACGTG 
GGGAACAGCC TGGCGCACGT GGAGCACCTC CTGAAGGATC TCACGAGCTT CGAGGAGAAG 
TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC TTGTCCCTGG AGGGTGAGCA GCTCATCGGC 
AACAAGCACT ATGCGGTGGA CTCCATCCGC CCCAAGTGCC ACGAGCTGCA GCACCTCTGC 
GACCAGTTTG CCGCCGAGGT CGGGCGGAGG AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG 
CACAGCCTCC TGGAGGCGTC CATGAAGTGG TGCGATGAAG GCATCTACCT GCTGGCCTCC 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCAGAGG CAGCCCTCCA GGAGATCGAG 
AAGTTTCTGG AGACCGGCGC AGAAAATAAG ATCCAGGAGC TCAATAAAAT TTACCAGGAC 
TACGAACTCA TCCTCACCCA AGACCTGAGG GAACACGTGC AGAAGGTCTT CCAGAAGCAA 
GAGAGCATGG AAGAGATGTT CCACCGGAGG CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC 
GCCTCCCCAG GTATCCGGAG AGGTTCTGAG AACAGCAGTT GCGAGGGCAG CGCGCAGAGA 
GGGCCCTACA GGAGGGCCAA GAGTGAAATG AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC 
ACGGGGGACG AGGAGGAGAG CCTGGCCATC CTGCGGAGGC ACGTCATGAA CGAGCTCCTT 
GACACGGAAC GGGTCTACGT AGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG 
ATGGACAACC CGTTAATGAC ACATCTCATC TCAACAGGCC TTCAAAACAA GAAGGATGTT 
CTATTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GAATATTCCT GAGGGAGCTA 
GAAAACTACA TAGACTGCCC AGAGCTGGTC GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG 
TTCCAGATGT ACGAGAAGTA CTGTCAGAAC AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG 
TGCGCCGACT GCCCCTTCTT CCAGGAATGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG GCCGAGGACT TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TCATCGCCAT CACGGGCTAT 
GACGGGAATC TGAGCGACCT GGGGAAGCTG CTCATGCAGG GCTCCTTCAG CGTCTGGACG 
GACCACAAGA AGGGCCACAG CAAGGTGAAG GACCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCGGTGCTC TTCTGCAAGA AGAGAGAGGA GAACGGGGAG 
GGCTATGAGA AAGCGCCTTC CTACAGCTAC AAGCAGTCCC TGAACATGGC GGCGGTTGGC 
ATAACAGAGA ACGTGAAGGG CGACGTGAGG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTTTACA TCATACAGGC ACCAACTCCC GAAATCAAAG CGGCGTGGGT GAGCGAAATC 
CGGAAGCTGC TGACCAGCCA GCTGCAGGCC TTCAGAGAAG CCAGCCAGCA CCGCGTACTG 
GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC 
ACAAGAAACG TCAAAAAGCT GGAAGAAAGG AAGACCGACC CCCTCAGCCT GGAAGGATAC 
GTGAGCTCGA CATCGCTGCC AAAGGCCCCC GAGAAGGGCA GAGATGACAC GGTCACTAAC 
TCTACCTCAG AAAGCTCTGC ACTTTCCAAA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCCACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA 
AAGAGCGACC CCACGCCTTT TGGTTTACGA GGTTGGAGCA AAACGTCTCA GCCCTCCGAG 
GCCCCTGAGG ACAACGACGG CTGGTCGAGT GCTGAGGAGC CCCTTAATTC CTCGGACGCA 
GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG CTGGTCCCGG GGAGGTTCAC AGTTGTGGCG 
GATGACGAGA AGGGCAGTCC TGACACGCTG GTCATGAGGA GCGGGGACCT GGTGGAGGTG 
GTCCAGGAGG GCGAGGACTG CCTCTGGTAT GTCAGGAACC TGACCTCCAG CAAGGAGGGC 
TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG 
AGCAGCTCAG AGTCCAGCGC AGGGTCCACC CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG 
AGCTGCGGGG TCCCCCTCTC AGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477636.1
CDS	123..3758
Translation	MFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621868.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621868.1

ORF Insert Sequence:

ATGTTTGACT GCTGGAGATT CATCCTGTGC AAGAAAACCG GACGCGACGA CTGTTCTTCC 
TCGCCGCGAT CCAATGAGGC CAAAACGGAG GAGAGTGACC ATTGGATTGA TGTCCCAGAC 
GGCATCAGGC TCGTTGGAGA GCAGCGCGAG GGGATGGCCA CACCCACAGA TGAAATCATG 
CACCAGGACA TCACCCCGCT CTGCGCCGCC GACATCCAGG ACCAGCTGAG GAAGCGCTTT 
GCCTATCTGT CCGGCGGGCG GGGGCAGGAC GGCAGCCCCG TCATCACCTT CCCGGACTAC 
CCGGCCTTCA GCGACGTCCC AGACAAGGAG TTCCAGAATG TCATGACCTA TCTCACCAGC 
ATCCCCAGCT TACAAGATGC TGGCATTGGG TTCATCCTGG TCATAGACCG AAGGCAGGAC 
AAATGGACCT CGGTGAAGGC ATCAGTCCTG CGAATAGCAG CGTCCTTCCC GGCAAACCTC 
CAGCGCGTCC TTGTCCTGCG CCCAACGGGA TTTTTCCAGA GGACCCTCTC TGACCTCGCT 
TTCAGATTCA ACAGAGATGA CTTTAAGATG AAGGTACCGG TCACAATGCT GAGCTCAGTA 
CCAGAATTGC ACGATTACAT TGACAAGTCC CAGCTGACGG AGGACCTGGG CGGGACCCTG 
GATTACTGCC ACACCAAGTG GTTGTGTCAC CGCACAGCCA TTGAGAGTTT TGCTGTCATG 
GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC TTCGGGACAG AGCTGGCCGA GACGGAACTG 
CCCAACGACG TCCCATCCAC AAGCTCTGTG CTCATCGCAC ACACGGAGAA GAAGCACAAA 
GCAAAGGAGA ACATGAGGCT GGCGCTGGAC GAGGGGCGGA GCATCCTGGA GAGCATCAGG 
GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC CCGAACCAGG ACCAGCTGGA CAATCAGACC 
ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT GAAACCGAGG CCGCCTTTGA TGAGTTTTGG 
GCAAAGCATC AGCAAAAACT CGAACAGTGT CTGCAGCTCC GGCATTTTGA GCAGGATTTC 
CGAGAGGTCA AAGCCGCCTT GGACACGTTG GCCCAGAAGA TAGTGACCTT CACCGACGTG 
GGGAACAGCC TGGCGCACGT GGAGCACCTC CTGAAGGATC TCACGAGCTT CGAGGAGAAG 
TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC TTGTCCCTGG AGGGTGAGCA GCTCATCGGC 
AACAAGCACT ATGCGGTGGA CTCCATCCGC CCCAAGTGCC ACGAGCTGCA GCACCTCTGC 
GACCAGTTTG CCGCCGAGGT CGGGCGGAGG AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG 
CACAGCCTCC TGGAGGCGTC CATGAAGTGG TGCGATGAAG GCATCTACCT GCTGGCCTCC 
CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC GGCGCAGAGG CAGCCCTCCA GGAGATCGAG 
AAGTTTCTGG AGACCGGCGC AGAAAATAAG ATCCAGGAGC TCAATAAAAT TTACCAGGAC 
TACGAACTCA TCCTCACCCA AGACCTGAGG GAACACGTGC AGAAGGTCTT CCAGAAGCAA 
GAGAGCATGG AAGAGATGTT CCACCGGAGG CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG 
CAGACGCGGC CCGTGCAGCC GGTGGCCCCC CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC 
GCCTCCCCAG GTATCCGGAG AGGTTCTGAG AACAGCAGTT GCGAGGGCAG CGCGCAGAGA 
GGGCCCTACA GGAGGGCCAA GAGTGAAATG AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC 
ACGGGGGACG AGGAGGAGAG CCTGGCCATC CTGCGGAGGC ACGTCATGAA CGAGCTCCTT 
GACACGGAAC GGGTCTACGT AGAGGAGCTG CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG 
ATGGACAACC CGTTAATGAC ACATCTCATC TCAACAGGCC TTCAAAACAA GAAGGATGTT 
CTATTTGGAA ACATGGAAGA AATTTACCAC TTTCATAACA GAATATTCCT GAGGGAGCTA 
GAAAACTACA TAGACTGCCC AGAGCTGGTC GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG 
TTCCAGATGT ACGAGAAGTA CTGTCAGAAC AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG 
TGCGCCGACT GCCCCTTCTT CCAGGAATGC CAGAAGAAGC TGGACCACAA GCTGAGCCTG 
GACTCCTACC TGCTGAAGCC AGTCCAGAGG ATCACCAAGT ACCAGCTGCT GCTCAAGGAA 
ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG GCCGAGGACT TGCAGGAGGC GCTGAGCTCC 
ATCCTGGGCA TCCTCAAGGC CGTGAACGAC TCCATGCACC TCATCGCCAT CACGGGCTAT 
GACGGGAATC TGAGCGACCT GGGGAAGCTG CTCATGCAGG GCTCCTTCAG CGTCTGGACG 
GACCACAAGA AGGGCCACAG CAAGGTGAAG GACCTGGCCA GGTTCAAGCC CATGCAGCGG 
CACCTGTTCC TGCACGAGAA GGCGGTGCTC TTCTGCAAGA AGAGAGAGGA GAACGGGGAG 
GGCTATGAGA AAGCGCCTTC CTACAGCTAC AAGCAGTCCC TGAACATGGC GGCGGTTGGC 
ATAACAGAGA ACGTGAAGGG CGACGTGAGG AAGTTTGAGA TCTGGTACAA CGCCCGGGAG 
GAGGTTTACA TCATACAGGC ACCAACTCCC GAAATCAAAG CGGCGTGGGT GAGCGAAATC 
CGGAAGCTGC TGACCAGCCA GCTGCAGGCC TTCAGAGAAG CCAGCCAGCA CCGCGTACTG 
GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC 
ACAAGAAACG TCAAAAAGCT GGAAGAAAGG AAGACCGACC CCCTCAGCCT GGAAGGATAC 
GTGAGCTCGA CATCGCTGCC AAAGGCCCCC GAGAAGGGCA GAGATGACAC GGTCACTAAC 
TCTACCTCAG AAAGCTCTGC ACTTTCCAAA AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC 
CTCAAAGGTC AGAAAGCTTC TCCCACCAGT CCTGACAAAA AAGCTAAACG GCACGAAGTA 
AAGAGCGACC CCACGCCTTT TGGTTTACGA GGTTGGAGCA AAACGTCTCA GCCCTCCGAG 
GCCCCTGAGG ACAACGACGG CTGGTCGAGT GCTGAGGAGC CCCTTAATTC CTCGGACGCA 
GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG CTGGTCCCGG GGAGGTTCAC AGTTGTGGCG 
GATGACGAGA AGGGCAGTCC TGACACGCTG GTCATGAGGA GCGGGGACCT GGTGGAGGTG 
GTCCAGGAGG GCGAGGACTG CCTCTGGTAT GTCAGGAACC TGACCTCCAG CAAGGAGGGC 
TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG 
AGCAGCTCAG AGTCCAGCGC AGGGTCCACC CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG 
AGCTGCGGGG TCCCCCTCTC AGACCTGCAG GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621870.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477638.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA.

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CloneID	OHb52013
Clone ID Related Accession (Same CDS sequence)	XM_023621870.1
Accession Version	XM_023621870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG GTTGGAGCAA AACGTCTCAG 
CCCTCCGAGG CCCCTGAGGA CAACGACGGC TGGTCGAGTG CTGAGGAGCC CCTTAATTCC 
TCGGACGCAG AGGAAGAAGG CGGAGTGGGC CCCAGGAAGC TGGTCCCGGG GAGGTTCACA 
GTTGTGGCGG ATGACGAGAA GGGCAGTCCT GACACGCTGG TCATGAGGAG CGGGGACCTG 
GTGGAGGTGG TCCAGGAGGG CGAGGACTGC CTCTGGTATG TCAGGAACCT GACCTCCAGC 
AAGGAGGGCT GGGTGCCGGC CGGCAGCCTG TCCGCGCGCC TCGGCAAGTC CAGCTCAGCT 
CAGTGCTTGA GCAGCTCAGA GTCCAGCGCA GGGTCCACCC TGCTGAGCAG CTCCTCCAGC 
TGCAGTGAGA GCTGCGGGGT CCCCCTCTCA GACCTGCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477638.1
CDS	16..3540
Translation	MVQCPRPPPGQAGPTLGRLMFDCWRFILCKKTGRDDCSSSPRSNEAKTEESDHWIDVPDGIRLVGEQREGMATPTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621870.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621870.1

ORF Insert Sequence:

ATGGTGCAGT GTCCTCGGCC CCCTCCGGGG CAGGCAGGAC CCACCCTTGG CAGACTCATG 
TTTGACTGCT GGAGATTCAT CCTGTGCAAG AAAACCGGAC GCGACGACTG TTCTTCCTCG 
CCGCGATCCA ATGAGGCCAA AACGGAGGAG AGTGACCATT GGATTGATGT CCCAGACGGC 
ATCAGGCTCG TTGGAGAGCA GCGCGAGGGG ATGGCCACAC CCACAGATGA AATCATGCAC 
CAGGACATCA CCCCGCTCTG CGCCGCCGAC ATCCAGGACC AGCTGAGGAA GCGCTTTGCC 
TATCTGTCCG GCGGGCGGGG GCAGGACGGC AGCCCCGTCA TCACCTTCCC GGACTACCCG 
GCCTTCAGCG ACGTCCCAGA CAAGGAGTTC CAGAATGTCA TGACCTATCT CACCAGCATC 
CCCAGCTTAC AAGATGCTGG CATTGGGTTC ATCCTGGTCA TAGACCGAAG GCAGGACAAA 
TGGACCTCGG TGAAGGCATC AGTCCTGCGA ATAGCAGCGT CCTTCCCGGC AAACCTCCAG 
CGCGTCCTTG TCCTGCGCCC AACGGGATTT TTCCAGAGGA CCCTCTCTGA CCTCGCTTTC 
AGATTCAACA GAGATGACTT TAAGATGAAG GTACCGGTCA CAATGCTGAG CTCAGTACCA 
GAATTGCACG ATTACATTGA CAAGTCCCAG CTGACGGAGG ACCTGGGCGG GACCCTGGAT 
TACTGCCACA CCAAGTGGTT GTGTCACCGC ACAGCCATTG AGAGTTTTGC TGTCATGGTG 
AAGCAGACGG CTCAGATGCT GCAGTCCTTC GGGACAGAGC TGGCCGAGAC GGAACTGCCC 
AACGACGTCC CATCCACAAG CTCTGTGCTC ATCGCACACA CGGAGAAGAA GCACAAAGCA 
AAGGAGAACA TGAGGCTGGC GCTGGACGAG GGGCGGAGCA TCCTGGAGAG CATCAGGGAG 
CCTCTGGCCA GGAGCACGGA GCAGAGCCCG AACCAGGACC AGCTGGACAA TCAGACCACC 
GTGCAGAGGC TCCTGGCCCA GCTGAATGAA ACCGAGGCCG CCTTTGATGA GTTTTGGGCA 
AAGCATCAGC AAAAACTCGA ACAGTGTCTG CAGCTCCGGC ATTTTGAGCA GGATTTCCGA 
GAGGTCAAAG CCGCCTTGGA CACGTTGGCC CAGAAGATAG TGACCTTCAC CGACGTGGGG 
AACAGCCTGG CGCACGTGGA GCACCTCCTG AAGGATCTCA CGAGCTTCGA GGAGAAGTCC 
AGTGCAGCCG TGGAGCGGGC CCGCGCCTTG TCCCTGGAGG GTGAGCAGCT CATCGGCAAC 
AAGCACTATG CGGTGGACTC CATCCGCCCC AAGTGCCACG AGCTGCAGCA CCTCTGCGAC 
CAGTTTGCCG CCGAGGTCGG GCGGAGGAGG GGGCTGCTCG GCAAGTCGCT GGAGCTGCAC 
AGCCTCCTGG AGGCGTCCAT GAAGTGGTGC GATGAAGGCA TCTACCTGCT GGCCTCCCAG 
CCTGTGGACA AGTGCCAGTC CCAGGACGGC GCAGAGGCAG CCCTCCAGGA GATCGAGAAG 
TTTCTGGAGA CCGGCGCAGA AAATAAGATC CAGGAGCTCA ATAAAATTTA CCAGGACTAC 
GAACTCATCC TCACCCAAGA CCTGAGGGAA CACGTGCAGA AGGTCTTCCA GAAGCAAGAG 
AGCATGGAAG AGATGTTCCA CCGGAGGCAG GCGAGCCTGA AGAAGCTGGC GGCCAAGCAG 
ACGCGGCCCG TGCAGCCGGT GGCCCCCCGG CCCGAGGCGC TCGCCAAGTC GCCCTGCGCC 
TCCCCAGGTA TCCGGAGAGG TTCTGAGAAC AGCAGTTGCG AGGGCAGCGC GCAGAGAGGG 
CCCTACAGGA GGGCCAAGAG TGAAATGAGT GAGGGCCGGC AGGGCCGAGG CAGCTCCACG 
GGGGACGAGG AGGAGAGCCT GGCCATCCTG CGGAGGCACG TCATGAACGA GCTCCTTGAC 
ACGGAACGGG TCTACGTAGA GGAGCTGCTG TGCGTCCTGG AGGGCTACGC TGCCGAGATG 
GACAACCCGT TAATGACACA TCTCATCTCA ACAGGCCTTC AAAACAAGAA GGATGTTCTA 
TTTGGAAACA TGGAAGAAAT TTACCACTTT CATAACAGAA TATTCCTGAG GGAGCTAGAA 
AACTACATAG ACTGCCCAGA GCTGGTCGGA AGGTGTTTTC TAGAAAGGCT GGAGGAGTTC 
CAGATGTACG AGAAGTACTG TCAGAACAAG CCGCGTTCCG AGAGCGTGTG GCGGCAGTGC 
GCCGACTGCC CCTTCTTCCA GGAATGCCAG AAGAAGCTGG ACCACAAGCT GAGCCTGGAC 
TCCTACCTGC TGAAGCCAGT CCAGAGGATC ACCAAGTACC AGCTGCTGCT CAAGGAAATG 
CTGAAATACA GCAAGAGCTG TGAGGGGGCC GAGGACTTGC AGGAGGCGCT GAGCTCCATC 
CTGGGCATCC TCAAGGCCGT GAACGACTCC ATGCACCTCA TCGCCATCAC GGGCTATGAC 
GGGAATCTGA GCGACCTGGG GAAGCTGCTC ATGCAGGGCT CCTTCAGCGT CTGGACGGAC 
CACAAGAAGG GCCACAGCAA GGTGAAGGAC CTGGCCAGGT TCAAGCCCAT GCAGCGGCAC 
CTGTTCCTGC ACGAGAAGGC GGTGCTCTTC TGCAAGAAGA GAGAGGAGAA CGGGGAGGGC 
TATGAGAAAG CGCCTTCCTA CAGCTACAAG CAGTCCCTGA ACATGGCGGC GGTTGGCATA 
ACAGAGAACG TGAAGGGCGA CGTGAGGAAG TTTGAGATCT GGTACAACGC CCGGGAGGAG 
GTTTACATCA TACAGGCACC AACTCCCGAA ATCAAAGCGG CGTGGGTGAG CGAAATCCGG 
AAGCTGCTGA CCAGCCAGCT GCAGGCCTTC AGAGAAGCCA GCCAGCACCG CGTACTGGAG 
CAGTCCCAGA GCCTGCCTCT GCCTGCAACG GCCAGCGCAA GTCCCTCGAA AGGGAACACA 
AGAAACGTCA AAAAGCTGGA AGAAAGGAAG ACCGACCCCC TCAGCCTGGA AGGATACGTG 
AGCTCGACAT CGCTGCCAAA GGCCCCCGAG AAGGGCAGAG GTTGGAGCAA AACGTCTCAG 
CCCTCCGAGG CCCCTGAGGA CAACGACGGC TGGTCGAGTG CTGAGGAGCC CCTTAATTCC 
TCGGACGCAG AGGAAGAAGG CGGAGTGGGC CCCAGGAAGC TGGTCCCGGG GAGGTTCACA 
GTTGTGGCGG ATGACGAGAA GGGCAGTCCT GACACGCTGG TCATGAGGAG CGGGGACCTG 
GTGGAGGTGG TCCAGGAGGG CGAGGACTGC CTCTGGTATG TCAGGAACCT GACCTCCAGC 
AAGGAGGGCT GGGTGCCGGC CGGCAGCCTG TCCGCGCGCC TCGGCAAGTC CAGCTCAGCT 
CAGTGCTTGA GCAGCTCAGA GTCCAGCGCA GGGTCCACCC TGCTGAGCAG CTCCTCCAGC 
TGCAGTGAGA GCTGCGGGGT CCCCCTCTCA GACCTGCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621871.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477639.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

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CloneID	OHb52014
Clone ID Related Accession (Same CDS sequence)	XM_023621871.1
Accession Version	XM_023621871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACC GAAGGGCTGC CCGCATCTCC GTGCCGGAAA TGAAAAGCTA CTACATTTAT 
TCTGAGTGGT GCTCCTGGCT GCTCTCTGTT GCTGAGGATG AAATCATGCA CCAGGACATC 
ACCCCGCTCT GCGCCGCCGA CATCCAGGAC CAGCTGAGGA AGCGCTTTGC CTATCTGTCC 
GGCGGGCGGG GGCAGGACGG CAGCCCCGTC ATCACCTTCC CGGACTACCC GGCCTTCAGC 
GACGTCCCAG ACAAGGAGTT CCAGAATGTC ATGACCTATC TCACCAGCAT CCCCAGCTTA 
CAAGATGCTG GCATTGGGTT CATCCTGGTC ATAGACCGAA GGCAGGACAA ATGGACCTCG 
GTGAAGGCAT CAGTCCTGCG AATAGCAGCG TCCTTCCCGG CAAACCTCCA GCGCGTCCTT 
GTCCTGCGCC CAACGGGATT TTTCCAGAGG ACCCTCTCTG ACCTCGCTTT CAGATTCAAC 
AGAGATGACT TTAAGATGAA GGTACCGGTC ACAATGCTGA GCTCAGTACC AGAATTGCAC 
GATTACATTG ACAAGTCCCA GCTGACGGAG GACCTGGGCG GGACCCTGGA TTACTGCCAC 
ACCAAGTGGT TGTGTCACCG CACAGCCATT GAGAGTTTTG CTGTCATGGT GAAGCAGACG 
GCTCAGATGC TGCAGTCCTT CGGGACAGAG CTGGCCGAGA CGGAACTGCC CAACGACGTC 
CCATCCACAA GCTCTGTGCT CATCGCACAC ACGGAGAAGA AGCACAAAGC AAAGGAGAAC 
ATGAGGCTGG CGCTGGACGA GGGGCGGAGC ATCCTGGAGA GCATCAGGGA GCCTCTGGCC 
AGGAGCACGG AGCAGAGCCC GAACCAGGAC CAGCTGGACA ATCAGACCAC CGTGCAGAGG 
CTCCTGGCCC AGCTGAATGA AACCGAGGCC GCCTTTGATG AGTTTTGGGC AAAGCATCAG 
CAAAAACTCG AACAGTGTCT GCAGCTCCGG CATTTTGAGC AGGATTTCCG AGAGGTCAAA 
GCCGCCTTGG ACACGTTGGC CCAGAAGATA GTGACCTTCA CCGACGTGGG GAACAGCCTG 
GCGCACGTGG AGCACCTCCT GAAGGATCTC ACGAGCTTCG AGGAGAAGTC CAGTGCAGCC 
GTGGAGCGGG CCCGCGCCTT GTCCCTGGAG GGTGAGCAGC TCATCGGCAA CAAGCACTAT 
GCGGTGGACT CCATCCGCCC CAAGTGCCAC GAGCTGCAGC ACCTCTGCGA CCAGTTTGCC 
GCCGAGGTCG GGCGGAGGAG GGGGCTGCTC GGCAAGTCGC TGGAGCTGCA CAGCCTCCTG 
GAGGCGTCCA TGAAGTGGTG CGATGAAGGC ATCTACCTGC TGGCCTCCCA GCCTGTGGAC 
AAGTGCCAGT CCCAGGACGG CGCAGAGGCA GCCCTCCAGG AGATCGAGAA GTTTCTGGAG 
ACCGGCGCAG AAAATAAGAT CCAGGAGCTC AATAAAATTT ACCAGGACTA CGAACTCATC 
CTCACCCAAG ACCTGAGGGA ACACGTGCAG AAGGTCTTCC AGAAGCAAGA GAGCATGGAA 
GAGATGTTCC ACCGGAGGCA GGCGAGCCTG AAGAAGCTGG CGGCCAAGCA GACGCGGCCC 
GTGCAGCCGG TGGCCCCCCG GCCCGAGGCG CTCGCCAAGT CGCCCTGCGC CTCCCCAGGT 
ATCCGGAGAG GTTCTGAGAA CAGCAGTTGC GAGGGCAGCG CGCAGAGAGG GCCCTACAGG 
AGGGCCAAGA GTGAAATGAG TGAGGGCCGG CAGGGCCGAG GCAGCTCCAC GGGGGACGAG 
GAGGAGAGCC TGGCCATCCT GCGGAGGCAC GTCATGAACG AGCTCCTTGA CACGGAACGG 
GTCTACGTAG AGGAGCTGCT GTGCGTCCTG GAGGGCTACG CTGCCGAGAT GGACAACCCG 
TTAATGACAC ATCTCATCTC AACAGGCCTT CAAAACAAGA AGGATGTTCT ATTTGGAAAC 
ATGGAAGAAA TTTACCACTT TCATAACAGA ATATTCCTGA GGGAGCTAGA AAACTACATA 
GACTGCCCAG AGCTGGTCGG AAGGTGTTTT CTAGAAAGGC TGGAGGAGTT CCAGATGTAC 
GAGAAGTACT GTCAGAACAA GCCGCGTTCC GAGAGCGTGT GGCGGCAGTG CGCCGACTGC 
CCCTTCTTCC AGGAATGCCA GAAGAAGCTG GACCACAAGC TGAGCCTGGA CTCCTACCTG 
CTGAAGCCAG TCCAGAGGAT CACCAAGTAC CAGCTGCTGC TCAAGGAAAT GCTGAAATAC 
AGCAAGAGCT GTGAGGGGGC CGAGGACTTG CAGGAGGCGC TGAGCTCCAT CCTGGGCATC 
CTCAAGGCCG TGAACGACTC CATGCACCTC ATCGCCATCA CGGGCTATGA CGGGAATCTG 
AGCGACCTGG GGAAGCTGCT CATGCAGGGC TCCTTCAGCG TCTGGACGGA CCACAAGAAG 
GGCCACAGCA AGGTGAAGGA CCTGGCCAGG TTCAAGCCCA TGCAGCGGCA CCTGTTCCTG 
CACGAGAAGG CGGTGCTCTT CTGCAAGAAG AGAGAGGAGA ACGGGGAGGG CTATGAGAAA 
GCGCCTTCCT ACAGCTACAA GCAGTCCCTG AACATGGCGG CGGTTGGCAT AACAGAGAAC 
GTGAAGGGCG ACGTGAGGAA GTTTGAGATC TGGTACAACG CCCGGGAGGA GGTTTACATC 
ATACAGGCAC CAACTCCCGA AATCAAAGCG GCGTGGGTGA GCGAAATCCG GAAGCTGCTG 
ACCAGCCAGC TGCAGGCCTT CAGAGAAGCC AGCCAGCACC GCGTACTGGA GCAGTCCCAG 
AGCCTGCCTC TGCCTGCAAC GGCCAGCGCA AGTCCCTCGA AAGGGAACAC AAGAAACGTC 
AAAAAGCTGG AAGAAAGGAA GACCGACCCC CTCAGCCTGG AAGGATACGT GAGCTCGACA 
TCGCTGCCAA AGGCCCCCGA GAAGGGCAGA GATGACACGG TCACTAACTC TACCTCAGAA 
AGCTCTGCAC TTTCCAAAAA GCGCTTTACC CTGCAGGGTT TTGCTAACCT CAAAGGTCAG 
AAAGCTTCTC CCACCAGTCC TGACAAAAAA GCTAAACGGC ACGAAGTAAA GAGCGACCCC 
ACGCCTTTTG GTTTACGAGG TTGGAGCAAA ACGTCTCAGC CCTCCGAGGC CCCTGAGGAC 
AACGACGGCT GGTCGAGTGC TGAGGAGCCC CTTAATTCCT CGGACGCAGA GGAAGAAGGC 
GGAGTGGGCC CCAGGAAGCT GGTCCCGGGG AGGTTCACAG TTGTGGCGGA TGACGAGAAG 
GGCAGTCCTG ACACGCTGGT CATGAGGAGC GGGGACCTGG TGGAGGTGGT CCAGGAGGGC 
GAGGACTGCC TCTGGTATGT CAGGAACCTG ACCTCCAGCA AGGAGGGCTG GGTGCCGGCC 
GGCAGCCTGT CCGCGCGCCT CGGCAAGTCC AGCTCAGCTC AGTGCTTGAG CAGCTCAGAG 
TCCAGCGCAG GGTCCACCCT GCTGAGCAGC TCCTCCAGCT GCAGTGAGAG CTGCGGGGTC 
CCCCTCTCAG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477639.1
CDS	555..4118
Translation	MGDRRAARISVPEMKSYYIYSEWCSWLLSVAEDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621871.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621871.1

ORF Insert Sequence:

ATGGGGGACC GAAGGGCTGC CCGCATCTCC GTGCCGGAAA TGAAAAGCTA CTACATTTAT 
TCTGAGTGGT GCTCCTGGCT GCTCTCTGTT GCTGAGGATG AAATCATGCA CCAGGACATC 
ACCCCGCTCT GCGCCGCCGA CATCCAGGAC CAGCTGAGGA AGCGCTTTGC CTATCTGTCC 
GGCGGGCGGG GGCAGGACGG CAGCCCCGTC ATCACCTTCC CGGACTACCC GGCCTTCAGC 
GACGTCCCAG ACAAGGAGTT CCAGAATGTC ATGACCTATC TCACCAGCAT CCCCAGCTTA 
CAAGATGCTG GCATTGGGTT CATCCTGGTC ATAGACCGAA GGCAGGACAA ATGGACCTCG 
GTGAAGGCAT CAGTCCTGCG AATAGCAGCG TCCTTCCCGG CAAACCTCCA GCGCGTCCTT 
GTCCTGCGCC CAACGGGATT TTTCCAGAGG ACCCTCTCTG ACCTCGCTTT CAGATTCAAC 
AGAGATGACT TTAAGATGAA GGTACCGGTC ACAATGCTGA GCTCAGTACC AGAATTGCAC 
GATTACATTG ACAAGTCCCA GCTGACGGAG GACCTGGGCG GGACCCTGGA TTACTGCCAC 
ACCAAGTGGT TGTGTCACCG CACAGCCATT GAGAGTTTTG CTGTCATGGT GAAGCAGACG 
GCTCAGATGC TGCAGTCCTT CGGGACAGAG CTGGCCGAGA CGGAACTGCC CAACGACGTC 
CCATCCACAA GCTCTGTGCT CATCGCACAC ACGGAGAAGA AGCACAAAGC AAAGGAGAAC 
ATGAGGCTGG CGCTGGACGA GGGGCGGAGC ATCCTGGAGA GCATCAGGGA GCCTCTGGCC 
AGGAGCACGG AGCAGAGCCC GAACCAGGAC CAGCTGGACA ATCAGACCAC CGTGCAGAGG 
CTCCTGGCCC AGCTGAATGA AACCGAGGCC GCCTTTGATG AGTTTTGGGC AAAGCATCAG 
CAAAAACTCG AACAGTGTCT GCAGCTCCGG CATTTTGAGC AGGATTTCCG AGAGGTCAAA 
GCCGCCTTGG ACACGTTGGC CCAGAAGATA GTGACCTTCA CCGACGTGGG GAACAGCCTG 
GCGCACGTGG AGCACCTCCT GAAGGATCTC ACGAGCTTCG AGGAGAAGTC CAGTGCAGCC 
GTGGAGCGGG CCCGCGCCTT GTCCCTGGAG GGTGAGCAGC TCATCGGCAA CAAGCACTAT 
GCGGTGGACT CCATCCGCCC CAAGTGCCAC GAGCTGCAGC ACCTCTGCGA CCAGTTTGCC 
GCCGAGGTCG GGCGGAGGAG GGGGCTGCTC GGCAAGTCGC TGGAGCTGCA CAGCCTCCTG 
GAGGCGTCCA TGAAGTGGTG CGATGAAGGC ATCTACCTGC TGGCCTCCCA GCCTGTGGAC 
AAGTGCCAGT CCCAGGACGG CGCAGAGGCA GCCCTCCAGG AGATCGAGAA GTTTCTGGAG 
ACCGGCGCAG AAAATAAGAT CCAGGAGCTC AATAAAATTT ACCAGGACTA CGAACTCATC 
CTCACCCAAG ACCTGAGGGA ACACGTGCAG AAGGTCTTCC AGAAGCAAGA GAGCATGGAA 
GAGATGTTCC ACCGGAGGCA GGCGAGCCTG AAGAAGCTGG CGGCCAAGCA GACGCGGCCC 
GTGCAGCCGG TGGCCCCCCG GCCCGAGGCG CTCGCCAAGT CGCCCTGCGC CTCCCCAGGT 
ATCCGGAGAG GTTCTGAGAA CAGCAGTTGC GAGGGCAGCG CGCAGAGAGG GCCCTACAGG 
AGGGCCAAGA GTGAAATGAG TGAGGGCCGG CAGGGCCGAG GCAGCTCCAC GGGGGACGAG 
GAGGAGAGCC TGGCCATCCT GCGGAGGCAC GTCATGAACG AGCTCCTTGA CACGGAACGG 
GTCTACGTAG AGGAGCTGCT GTGCGTCCTG GAGGGCTACG CTGCCGAGAT GGACAACCCG 
TTAATGACAC ATCTCATCTC AACAGGCCTT CAAAACAAGA AGGATGTTCT ATTTGGAAAC 
ATGGAAGAAA TTTACCACTT TCATAACAGA ATATTCCTGA GGGAGCTAGA AAACTACATA 
GACTGCCCAG AGCTGGTCGG AAGGTGTTTT CTAGAAAGGC TGGAGGAGTT CCAGATGTAC 
GAGAAGTACT GTCAGAACAA GCCGCGTTCC GAGAGCGTGT GGCGGCAGTG CGCCGACTGC 
CCCTTCTTCC AGGAATGCCA GAAGAAGCTG GACCACAAGC TGAGCCTGGA CTCCTACCTG 
CTGAAGCCAG TCCAGAGGAT CACCAAGTAC CAGCTGCTGC TCAAGGAAAT GCTGAAATAC 
AGCAAGAGCT GTGAGGGGGC CGAGGACTTG CAGGAGGCGC TGAGCTCCAT CCTGGGCATC 
CTCAAGGCCG TGAACGACTC CATGCACCTC ATCGCCATCA CGGGCTATGA CGGGAATCTG 
AGCGACCTGG GGAAGCTGCT CATGCAGGGC TCCTTCAGCG TCTGGACGGA CCACAAGAAG 
GGCCACAGCA AGGTGAAGGA CCTGGCCAGG TTCAAGCCCA TGCAGCGGCA CCTGTTCCTG 
CACGAGAAGG CGGTGCTCTT CTGCAAGAAG AGAGAGGAGA ACGGGGAGGG CTATGAGAAA 
GCGCCTTCCT ACAGCTACAA GCAGTCCCTG AACATGGCGG CGGTTGGCAT AACAGAGAAC 
GTGAAGGGCG ACGTGAGGAA GTTTGAGATC TGGTACAACG CCCGGGAGGA GGTTTACATC 
ATACAGGCAC CAACTCCCGA AATCAAAGCG GCGTGGGTGA GCGAAATCCG GAAGCTGCTG 
ACCAGCCAGC TGCAGGCCTT CAGAGAAGCC AGCCAGCACC GCGTACTGGA GCAGTCCCAG 
AGCCTGCCTC TGCCTGCAAC GGCCAGCGCA AGTCCCTCGA AAGGGAACAC AAGAAACGTC 
AAAAAGCTGG AAGAAAGGAA GACCGACCCC CTCAGCCTGG AAGGATACGT GAGCTCGACA 
TCGCTGCCAA AGGCCCCCGA GAAGGGCAGA GATGACACGG TCACTAACTC TACCTCAGAA 
AGCTCTGCAC TTTCCAAAAA GCGCTTTACC CTGCAGGGTT TTGCTAACCT CAAAGGTCAG 
AAAGCTTCTC CCACCAGTCC TGACAAAAAA GCTAAACGGC ACGAAGTAAA GAGCGACCCC 
ACGCCTTTTG GTTTACGAGG TTGGAGCAAA ACGTCTCAGC CCTCCGAGGC CCCTGAGGAC 
AACGACGGCT GGTCGAGTGC TGAGGAGCCC CTTAATTCCT CGGACGCAGA GGAAGAAGGC 
GGAGTGGGCC CCAGGAAGCT GGTCCCGGGG AGGTTCACAG TTGTGGCGGA TGACGAGAAG 
GGCAGTCCTG ACACGCTGGT CATGAGGAGC GGGGACCTGG TGGAGGTGGT CCAGGAGGGC 
GAGGACTGCC TCTGGTATGT CAGGAACCTG ACCTCCAGCA AGGAGGGCTG GGTGCCGGCC 
GGCAGCCTGT CCGCGCGCCT CGGCAAGTCC AGCTCAGCTC AGTGCTTGAG CAGCTCAGAG 
TCCAGCGCAG GGTCCACCCT GCTGAGCAGC TCCTCCAGCT GCAGTGAGAG CTGCGGGGTC 
CCCCTCTCAG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_014732205.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587691.2 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHb52015
Clone ID Related Accession (Same CDS sequence)	XM_014732205.2
Accession Version	XM_014732205.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014732205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGA GGGGAGCGTC CCGGGGCCGC CAGCGCTGGC TCTGCCTGCT GCCCTTGGGA 
TGCCGGGGGG CCTCGAAGCG CCCACTTTCA GATGAAATCA TGCACCAGGA CATCACCCCG 
CTCTGCGCCG CCGACATCCA GGACCAGCTG AGGAAGCGCT TTGCCTATCT GTCCGGCGGG 
CGGGGGCAGG ACGGCAGCCC CGTCATCACC TTCCCGGACT ACCCGGCCTT CAGCGACGTC 
CCAGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG CTTACAAGAT 
GCTGGCATTG GGTTCATCCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCGGTGAAG 
GCATCAGTCC TGCGAATAGC AGCGTCCTTC CCGGCAAACC TCCAGCGCGT CCTTGTCCTG 
CGCCCAACGG GATTTTTCCA GAGGACCCTC TCTGACCTCG CTTTCAGATT CAACAGAGAT 
GACTTTAAGA TGAAGGTACC GGTCACAATG CTGAGCTCAG TACCAGAATT GCACGATTAC 
ATTGACAAGT CCCAGCTGAC GGAGGACCTG GGCGGGACCC TGGATTACTG CCACACCAAG 
TGGTTGTGTC ACCGCACAGC CATTGAGAGT TTTGCTGTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC AGAGCTGGCC GAGACGGAAC TGCCCAACGA CGTCCCATCC 
ACAAGCTCTG TGCTCATCGC ACACACGGAG AAGAAGCACA AAGCAAAGGA GAACATGAGG 
CTGGCGCTGG ACGAGGGGCG GAGCATCCTG GAGAGCATCA GGGAGCCTCT GGCCAGGAGC 
ACGGAGCAGA GCCCGAACCA GGACCAGCTG GACAATCAGA CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAAACCGA GGCCGCCTTT GATGAGTTTT GGGCAAAGCA TCAGCAAAAA 
CTCGAACAGT GTCTGCAGCT CCGGCATTTT GAGCAGGATT TCCGAGAGGT CAAAGCCGCC 
TTGGACACGT TGGCCCAGAA GATAGTGACC TTCACCGACG TGGGGAACAG CCTGGCGCAC 
GTGGAGCACC TCCTGAAGGA TCTCACGAGC TTCGAGGAGA AGTCCAGTGC AGCCGTGGAG 
CGGGCCCGCG CCTTGTCCCT GGAGGGTGAG CAGCTCATCG GCAACAAGCA CTATGCGGTG 
GACTCCATCC GCCCCAAGTG CCACGAGCTG CAGCACCTCT GCGACCAGTT TGCCGCCGAG 
GTCGGGCGGA GGAGGGGGCT GCTCGGCAAG TCGCTGGAGC TGCACAGCCT CCTGGAGGCG 
TCCATGAAGT GGTGCGATGA AGGCATCTAC CTGCTGGCCT CCCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCAGA GGCAGCCCTC CAGGAGATCG AGAAGTTTCT GGAGACCGGC 
GCAGAAAATA AGATCCAGGA GCTCAATAAA ATTTACCAGG ACTACGAACT CATCCTCACC 
CAAGACCTGA GGGAACACGT GCAGAAGGTC TTCCAGAAGC AAGAGAGCAT GGAAGAGATG 
TTCCACCGGA GGCAGGCGAG CCTGAAGAAG CTGGCGGCCA AGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCCGGCCCGA GGCGCTCGCC AAGTCGCCCT GCGCCTCCCC AGGTATCCGG 
AGAGGTTCTG AGAACAGCAG TTGCGAGGGC AGCGCGCAGA GAGGGCCCTA CAGGAGGGCC 
AAGAGTGAAA TGAGTGAGGG CCGGCAGGGC CGAGGCAGCT CCACGGGGGA CGAGGAGGAG 
AGCCTGGCCA TCCTGCGGAG GCACGTCATG AACGAGCTCC TTGACACGGA ACGGGTCTAC 
GTAGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCTGCCG AGATGGACAA CCCGTTAATG 
ACACATCTCA TCTCAACAGG CCTTCAAAAC AAGAAGGATG TTCTATTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGAATATTC CTGAGGGAGC TAGAAAACTA CATAGACTGC 
CCAGAGCTGG TCGGAAGGTG TTTTCTAGAA AGGCTGGAGG AGTTCCAGAT GTACGAGAAG 
TACTGTCAGA ACAAGCCGCG TTCCGAGAGC GTGTGGCGGC AGTGCGCCGA CTGCCCCTTC 
TTCCAGGAAT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAAG 
AGCTGTGAGG GGGCCGAGGA CTTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCC ATCACGGGCT ATGACGGGAA TCTGAGCGAC 
CTGGGGAAGC TGCTCATGCA GGGCTCCTTC AGCGTCTGGA CGGACCACAA GAAGGGCCAC 
AGCAAGGTGA AGGACCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCGGTGC TCTTCTGCAA GAAGAGAGAG GAGAACGGGG AGGGCTATGA GAAAGCGCCT 
TCCTACAGCT ACAAGCAGTC CCTGAACATG GCGGCGGTTG GCATAACAGA GAACGTGAAG 
GGCGACGTGA GGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTTTA CATCATACAG 
GCACCAACTC CCGAAATCAA AGCGGCGTGG GTGAGCGAAA TCCGGAAGCT GCTGACCAGC 
CAGCTGCAGG CCTTCAGAGA AGCCAGCCAG CACCGCGTAC TGGAGCAGTC CCAGAGCCTG 
CCTCTGCCTG CAACGGCCAG CGCAAGTCCC TCGAAAGGGA ACACAAGAAA CGTCAAAAAG 
CTGGAAGAAA GGAAGACCGA CCCCCTCAGC CTGGAAGGAT ACGTGAGCTC GACATCGCTG 
CCAAAGGCCC CCGAGAAGGG CAGAGATGAC ACGGTCACTA ACTCTACCTC AGAAAGCTCT 
GCACTTTCCA AAAAGCGCTT TACCCTGCAG GGTTTTGCTA ACCTCAAAGG TCAGAAAGCT 
TCTCCCACCA GTCCTGACAA AAAAGCTAAA CGGCACGAAG TAAAGAGCGA CCCCACGCCT 
TTTGGTTTAC GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC GGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACACGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587691.2
CDS	567..4124
Translation	MAQRGASRGRQRWLCLLPLGCRGASKRPLSDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732205.2
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732205.2

ORF Insert Sequence:

ATGGCCCAGA GGGGAGCGTC CCGGGGCCGC CAGCGCTGGC TCTGCCTGCT GCCCTTGGGA 
TGCCGGGGGG CCTCGAAGCG CCCACTTTCA GATGAAATCA TGCACCAGGA CATCACCCCG 
CTCTGCGCCG CCGACATCCA GGACCAGCTG AGGAAGCGCT TTGCCTATCT GTCCGGCGGG 
CGGGGGCAGG ACGGCAGCCC CGTCATCACC TTCCCGGACT ACCCGGCCTT CAGCGACGTC 
CCAGACAAGG AGTTCCAGAA TGTCATGACC TATCTCACCA GCATCCCCAG CTTACAAGAT 
GCTGGCATTG GGTTCATCCT GGTCATAGAC CGAAGGCAGG ACAAATGGAC CTCGGTGAAG 
GCATCAGTCC TGCGAATAGC AGCGTCCTTC CCGGCAAACC TCCAGCGCGT CCTTGTCCTG 
CGCCCAACGG GATTTTTCCA GAGGACCCTC TCTGACCTCG CTTTCAGATT CAACAGAGAT 
GACTTTAAGA TGAAGGTACC GGTCACAATG CTGAGCTCAG TACCAGAATT GCACGATTAC 
ATTGACAAGT CCCAGCTGAC GGAGGACCTG GGCGGGACCC TGGATTACTG CCACACCAAG 
TGGTTGTGTC ACCGCACAGC CATTGAGAGT TTTGCTGTCA TGGTGAAGCA GACGGCTCAG 
ATGCTGCAGT CCTTCGGGAC AGAGCTGGCC GAGACGGAAC TGCCCAACGA CGTCCCATCC 
ACAAGCTCTG TGCTCATCGC ACACACGGAG AAGAAGCACA AAGCAAAGGA GAACATGAGG 
CTGGCGCTGG ACGAGGGGCG GAGCATCCTG GAGAGCATCA GGGAGCCTCT GGCCAGGAGC 
ACGGAGCAGA GCCCGAACCA GGACCAGCTG GACAATCAGA CCACCGTGCA GAGGCTCCTG 
GCCCAGCTGA ATGAAACCGA GGCCGCCTTT GATGAGTTTT GGGCAAAGCA TCAGCAAAAA 
CTCGAACAGT GTCTGCAGCT CCGGCATTTT GAGCAGGATT TCCGAGAGGT CAAAGCCGCC 
TTGGACACGT TGGCCCAGAA GATAGTGACC TTCACCGACG TGGGGAACAG CCTGGCGCAC 
GTGGAGCACC TCCTGAAGGA TCTCACGAGC TTCGAGGAGA AGTCCAGTGC AGCCGTGGAG 
CGGGCCCGCG CCTTGTCCCT GGAGGGTGAG CAGCTCATCG GCAACAAGCA CTATGCGGTG 
GACTCCATCC GCCCCAAGTG CCACGAGCTG CAGCACCTCT GCGACCAGTT TGCCGCCGAG 
GTCGGGCGGA GGAGGGGGCT GCTCGGCAAG TCGCTGGAGC TGCACAGCCT CCTGGAGGCG 
TCCATGAAGT GGTGCGATGA AGGCATCTAC CTGCTGGCCT CCCAGCCTGT GGACAAGTGC 
CAGTCCCAGG ACGGCGCAGA GGCAGCCCTC CAGGAGATCG AGAAGTTTCT GGAGACCGGC 
GCAGAAAATA AGATCCAGGA GCTCAATAAA ATTTACCAGG ACTACGAACT CATCCTCACC 
CAAGACCTGA GGGAACACGT GCAGAAGGTC TTCCAGAAGC AAGAGAGCAT GGAAGAGATG 
TTCCACCGGA GGCAGGCGAG CCTGAAGAAG CTGGCGGCCA AGCAGACGCG GCCCGTGCAG 
CCGGTGGCCC CCCGGCCCGA GGCGCTCGCC AAGTCGCCCT GCGCCTCCCC AGGTATCCGG 
AGAGGTTCTG AGAACAGCAG TTGCGAGGGC AGCGCGCAGA GAGGGCCCTA CAGGAGGGCC 
AAGAGTGAAA TGAGTGAGGG CCGGCAGGGC CGAGGCAGCT CCACGGGGGA CGAGGAGGAG 
AGCCTGGCCA TCCTGCGGAG GCACGTCATG AACGAGCTCC TTGACACGGA ACGGGTCTAC 
GTAGAGGAGC TGCTGTGCGT CCTGGAGGGC TACGCTGCCG AGATGGACAA CCCGTTAATG 
ACACATCTCA TCTCAACAGG CCTTCAAAAC AAGAAGGATG TTCTATTTGG AAACATGGAA 
GAAATTTACC ACTTTCATAA CAGAATATTC CTGAGGGAGC TAGAAAACTA CATAGACTGC 
CCAGAGCTGG TCGGAAGGTG TTTTCTAGAA AGGCTGGAGG AGTTCCAGAT GTACGAGAAG 
TACTGTCAGA ACAAGCCGCG TTCCGAGAGC GTGTGGCGGC AGTGCGCCGA CTGCCCCTTC 
TTCCAGGAAT GCCAGAAGAA GCTGGACCAC AAGCTGAGCC TGGACTCCTA CCTGCTGAAG 
CCAGTCCAGA GGATCACCAA GTACCAGCTG CTGCTCAAGG AAATGCTGAA ATACAGCAAG 
AGCTGTGAGG GGGCCGAGGA CTTGCAGGAG GCGCTGAGCT CCATCCTGGG CATCCTCAAG 
GCCGTGAACG ACTCCATGCA CCTCATCGCC ATCACGGGCT ATGACGGGAA TCTGAGCGAC 
CTGGGGAAGC TGCTCATGCA GGGCTCCTTC AGCGTCTGGA CGGACCACAA GAAGGGCCAC 
AGCAAGGTGA AGGACCTGGC CAGGTTCAAG CCCATGCAGC GGCACCTGTT CCTGCACGAG 
AAGGCGGTGC TCTTCTGCAA GAAGAGAGAG GAGAACGGGG AGGGCTATGA GAAAGCGCCT 
TCCTACAGCT ACAAGCAGTC CCTGAACATG GCGGCGGTTG GCATAACAGA GAACGTGAAG 
GGCGACGTGA GGAAGTTTGA GATCTGGTAC AACGCCCGGG AGGAGGTTTA CATCATACAG 
GCACCAACTC CCGAAATCAA AGCGGCGTGG GTGAGCGAAA TCCGGAAGCT GCTGACCAGC 
CAGCTGCAGG CCTTCAGAGA AGCCAGCCAG CACCGCGTAC TGGAGCAGTC CCAGAGCCTG 
CCTCTGCCTG CAACGGCCAG CGCAAGTCCC TCGAAAGGGA ACACAAGAAA CGTCAAAAAG 
CTGGAAGAAA GGAAGACCGA CCCCCTCAGC CTGGAAGGAT ACGTGAGCTC GACATCGCTG 
CCAAAGGCCC CCGAGAAGGG CAGAGATGAC ACGGTCACTA ACTCTACCTC AGAAAGCTCT 
GCACTTTCCA AAAAGCGCTT TACCCTGCAG GGTTTTGCTA ACCTCAAAGG TCAGAAAGCT 
TCTCCCACCA GTCCTGACAA AAAAGCTAAA CGGCACGAAG TAAAGAGCGA CCCCACGCCT 
TTTGGTTTAC GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC GGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACACGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_005601347.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601404.2 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA.

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CloneID	OHb52016
Clone ID Related Accession (Same CDS sequence)	XM_005601347.3
Accession Version	XM_005601347.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005601347.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCCGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CAGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAACGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGATGACAC GGTCACTAAC TCTACCTCAG AAAGCTCTGC ACTTTCCAAA 
AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC CTCAAAGGTC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAACG GCACGAAGTA AAGAGCGACC CCACGCCTTT TGGTTTACGA 
GGTTGGAGCA AAACGTCTCA GCCCTCCGAG GCCCCTGAGG ACAACGACGG CTGGTCGAGT 
GCTGAGGAGC CCCTTAATTC CTCGGACGCA GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG 
CTGGTCCCGG GGAGGTTCAC AGTTGTGGCG GATGACGAGA AGGGCAGTCC TGACACGCTG 
GTCATGAGGA GCGGGGACCT GGTGGAGGTG GTCCAGGAGG GCGAGGACTG CCTCTGGTAT 
GTCAGGAACC TGACCTCCAG CAAGGAGGGC TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC 
CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG AGCAGCTCAG AGTCCAGCGC AGGGTCCACC 
CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG AGCTGCGGGG TCCCCCTCTC AGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601404.2
CDS	449..3994
Translation	MRFWLRNEEMALEEMVQRLNAVSARTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_005601347.3
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601347.3

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCCGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CAGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAACGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGATGACAC GGTCACTAAC TCTACCTCAG AAAGCTCTGC ACTTTCCAAA 
AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC CTCAAAGGTC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAACG GCACGAAGTA AAGAGCGACC CCACGCCTTT TGGTTTACGA 
GGTTGGAGCA AAACGTCTCA GCCCTCCGAG GCCCCTGAGG ACAACGACGG CTGGTCGAGT 
GCTGAGGAGC CCCTTAATTC CTCGGACGCA GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG 
CTGGTCCCGG GGAGGTTCAC AGTTGTGGCG GATGACGAGA AGGGCAGTCC TGACACGCTG 
GTCATGAGGA GCGGGGACCT GGTGGAGGTG GTCCAGGAGG GCGAGGACTG CCTCTGGTAT 
GTCAGGAACC TGACCTCCAG CAAGGAGGGC TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC 
CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG AGCAGCTCAG AGTCCAGCGC AGGGTCCACC 
CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG AGCTGCGGGG TCCCCCTCTC AGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_005601348.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601405.2 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.

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CloneID	OHb52017
Clone ID Related Accession (Same CDS sequence)	XM_005601348.3
Accession Version	XM_005601348.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005601348.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTAA GCCTCATCTC CGTGCTGTCG CAGGATATCT CCGGGCTGTG GCTCCTGCTG 
AAGGCGAGCA CAGATGAAAT CATGCACCAG GACATCACCC CGCTCTGCGC CGCCGACATC 
CAGGACCAGC TGAGGAAGCG CTTTGCCTAT CTGTCCGGCG GGCGGGGGCA GGACGGCAGC 
CCCGTCATCA CCTTCCCGGA CTACCCGGCC TTCAGCGACG TCCCAGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGCTTACAAG ATGCTGGCAT TGGGTTCATC 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCGGTGA AGGCATCAGT CCTGCGAATA 
GCAGCGTCCT TCCCGGCAAA CCTCCAGCGC GTCCTTGTCC TGCGCCCAAC GGGATTTTTC 
CAGAGGACCC TCTCTGACCT CGCTTTCAGA TTCAACAGAG ATGACTTTAA GATGAAGGTA 
CCGGTCACAA TGCTGAGCTC AGTACCAGAA TTGCACGATT ACATTGACAA GTCCCAGCTG 
ACGGAGGACC TGGGCGGGAC CCTGGATTAC TGCCACACCA AGTGGTTGTG TCACCGCACA 
GCCATTGAGA GTTTTGCTGT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACAGAGCTGG CCGAGACGGA ACTGCCCAAC GACGTCCCAT CCACAAGCTC TGTGCTCATC 
GCACACACGG AGAAGAAGCA CAAAGCAAAG GAGAACATGA GGCTGGCGCT GGACGAGGGG 
CGGAGCATCC TGGAGAGCAT CAGGGAGCCT CTGGCCAGGA GCACGGAGCA GAGCCCGAAC 
CAGGACCAGC TGGACAATCA GACCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAAACC 
GAGGCCGCCT TTGATGAGTT TTGGGCAAAG CATCAGCAAA AACTCGAACA GTGTCTGCAG 
CTCCGGCATT TTGAGCAGGA TTTCCGAGAG GTCAAAGCCG CCTTGGACAC GTTGGCCCAG 
AAGATAGTGA CCTTCACCGA CGTGGGGAAC AGCCTGGCGC ACGTGGAGCA CCTCCTGAAG 
GATCTCACGA GCTTCGAGGA GAAGTCCAGT GCAGCCGTGG AGCGGGCCCG CGCCTTGTCC 
CTGGAGGGTG AGCAGCTCAT CGGCAACAAG CACTATGCGG TGGACTCCAT CCGCCCCAAG 
TGCCACGAGC TGCAGCACCT CTGCGACCAG TTTGCCGCCG AGGTCGGGCG GAGGAGGGGG 
CTGCTCGGCA AGTCGCTGGA GCTGCACAGC CTCCTGGAGG CGTCCATGAA GTGGTGCGAT 
GAAGGCATCT ACCTGCTGGC CTCCCAGCCT GTGGACAAGT GCCAGTCCCA GGACGGCGCA 
GAGGCAGCCC TCCAGGAGAT CGAGAAGTTT CTGGAGACCG GCGCAGAAAA TAAGATCCAG 
GAGCTCAATA AAATTTACCA GGACTACGAA CTCATCCTCA CCCAAGACCT GAGGGAACAC 
GTGCAGAAGG TCTTCCAGAA GCAAGAGAGC ATGGAAGAGA TGTTCCACCG GAGGCAGGCG 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCCGGCCC 
GAGGCGCTCG CCAAGTCGCC CTGCGCCTCC CCAGGTATCC GGAGAGGTTC TGAGAACAGC 
AGTTGCGAGG GCAGCGCGCA GAGAGGGCCC TACAGGAGGG CCAAGAGTGA AATGAGTGAG 
GGCCGGCAGG GCCGAGGCAG CTCCACGGGG GACGAGGAGG AGAGCCTGGC CATCCTGCGG 
AGGCACGTCA TGAACGAGCT CCTTGACACG GAACGGGTCT ACGTAGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCTGC CGAGATGGAC AACCCGTTAA TGACACATCT CATCTCAACA 
GGCCTTCAAA ACAAGAAGGA TGTTCTATTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGAATAT TCCTGAGGGA GCTAGAAAAC TACATAGACT GCCCAGAGCT GGTCGGAAGG 
TGTTTTCTAG AAAGGCTGGA GGAGTTCCAG ATGTACGAGA AGTACTGTCA GAACAAGCCG 
CGTTCCGAGA GCGTGTGGCG GCAGTGCGCC GACTGCCCCT TCTTCCAGGA ATGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA AGAGCTGTGA GGGGGCCGAG 
GACTTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CCATCACGGG CTATGACGGG AATCTGAGCG ACCTGGGGAA GCTGCTCATG 
CAGGGCTCCT TCAGCGTCTG GACGGACCAC AAGAAGGGCC ACAGCAAGGT GAAGGACCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCGGT GCTCTTCTGC 
AAGAAGAGAG AGGAGAACGG GGAGGGCTAT GAGAAAGCGC CTTCCTACAG CTACAAGCAG 
TCCCTGAACA TGGCGGCGGT TGGCATAACA GAGAACGTGA AGGGCGACGT GAGGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTT TACATCATAC AGGCACCAAC TCCCGAAATC 
AAAGCGGCGT GGGTGAGCGA AATCCGGAAG CTGCTGACCA GCCAGCTGCA GGCCTTCAGA 
GAAGCCAGCC AGCACCGCGT ACTGGAGCAG TCCCAGAGCC TGCCTCTGCC TGCAACGGCC 
AGCGCAAGTC CCTCGAAAGG GAACACAAGA AACGTCAAAA AGCTGGAAGA AAGGAAGACC 
GACCCCCTCA GCCTGGAAGG ATACGTGAGC TCGACATCGC TGCCAAAGGC CCCCGAGAAG 
GGCAGAGATG ACACGGTCAC TAACTCTACC TCAGAAAGCT CTGCACTTTC CAAAAAGCGC 
TTTACCCTGC AGGGTTTTGC TAACCTCAAA GGTCAGAAAG CTTCTCCCAC CAGTCCTGAC 
AAAAAAGCTA AACGGCACGA AGTAAAGAGC GACCCCACGC CTTTTGGTTT ACGAGGTTGG 
AGCAAAACGT CTCAGCCCTC CGAGGCCCCT GAGGACAACG ACGGCTGGTC GAGTGCTGAG 
GAGCCCCTTA ATTCCTCGGA CGCAGAGGAA GAAGGCGGAG TGGGCCCCAG GAAGCTGGTC 
CCGGGGAGGT TCACAGTTGT GGCGGATGAC GAGAAGGGCA GTCCTGACAC GCTGGTCATG 
AGGAGCGGGG ACCTGGTGGA GGTGGTCCAG GAGGGCGAGG ACTGCCTCTG GTATGTCAGG 
AACCTGACCT CCAGCAAGGA GGGCTGGGTG CCGGCCGGCA GCCTGTCCGC GCGCCTCGGC 
AAGTCCAGCT CAGCTCAGTG CTTGAGCAGC TCAGAGTCCA GCGCAGGGTC CACCCTGCTG 
AGCAGCTCCT CCAGCTGCAG TGAGAGCTGC GGGGTCCCCC TCTCAGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601405.2
CDS	2747..6286
Translation	MTLSLISVLSQDISGLWLLLKASTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_005601348.3
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601348.3

ORF Insert Sequence:

ATGACCTTAA GCCTCATCTC CGTGCTGTCG CAGGATATCT CCGGGCTGTG GCTCCTGCTG 
AAGGCGAGCA CAGATGAAAT CATGCACCAG GACATCACCC CGCTCTGCGC CGCCGACATC 
CAGGACCAGC TGAGGAAGCG CTTTGCCTAT CTGTCCGGCG GGCGGGGGCA GGACGGCAGC 
CCCGTCATCA CCTTCCCGGA CTACCCGGCC TTCAGCGACG TCCCAGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGCTTACAAG ATGCTGGCAT TGGGTTCATC 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCGGTGA AGGCATCAGT CCTGCGAATA 
GCAGCGTCCT TCCCGGCAAA CCTCCAGCGC GTCCTTGTCC TGCGCCCAAC GGGATTTTTC 
CAGAGGACCC TCTCTGACCT CGCTTTCAGA TTCAACAGAG ATGACTTTAA GATGAAGGTA 
CCGGTCACAA TGCTGAGCTC AGTACCAGAA TTGCACGATT ACATTGACAA GTCCCAGCTG 
ACGGAGGACC TGGGCGGGAC CCTGGATTAC TGCCACACCA AGTGGTTGTG TCACCGCACA 
GCCATTGAGA GTTTTGCTGT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACAGAGCTGG CCGAGACGGA ACTGCCCAAC GACGTCCCAT CCACAAGCTC TGTGCTCATC 
GCACACACGG AGAAGAAGCA CAAAGCAAAG GAGAACATGA GGCTGGCGCT GGACGAGGGG 
CGGAGCATCC TGGAGAGCAT CAGGGAGCCT CTGGCCAGGA GCACGGAGCA GAGCCCGAAC 
CAGGACCAGC TGGACAATCA GACCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAAACC 
GAGGCCGCCT TTGATGAGTT TTGGGCAAAG CATCAGCAAA AACTCGAACA GTGTCTGCAG 
CTCCGGCATT TTGAGCAGGA TTTCCGAGAG GTCAAAGCCG CCTTGGACAC GTTGGCCCAG 
AAGATAGTGA CCTTCACCGA CGTGGGGAAC AGCCTGGCGC ACGTGGAGCA CCTCCTGAAG 
GATCTCACGA GCTTCGAGGA GAAGTCCAGT GCAGCCGTGG AGCGGGCCCG CGCCTTGTCC 
CTGGAGGGTG AGCAGCTCAT CGGCAACAAG CACTATGCGG TGGACTCCAT CCGCCCCAAG 
TGCCACGAGC TGCAGCACCT CTGCGACCAG TTTGCCGCCG AGGTCGGGCG GAGGAGGGGG 
CTGCTCGGCA AGTCGCTGGA GCTGCACAGC CTCCTGGAGG CGTCCATGAA GTGGTGCGAT 
GAAGGCATCT ACCTGCTGGC CTCCCAGCCT GTGGACAAGT GCCAGTCCCA GGACGGCGCA 
GAGGCAGCCC TCCAGGAGAT CGAGAAGTTT CTGGAGACCG GCGCAGAAAA TAAGATCCAG 
GAGCTCAATA AAATTTACCA GGACTACGAA CTCATCCTCA CCCAAGACCT GAGGGAACAC 
GTGCAGAAGG TCTTCCAGAA GCAAGAGAGC ATGGAAGAGA TGTTCCACCG GAGGCAGGCG 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCCGGCCC 
GAGGCGCTCG CCAAGTCGCC CTGCGCCTCC CCAGGTATCC GGAGAGGTTC TGAGAACAGC 
AGTTGCGAGG GCAGCGCGCA GAGAGGGCCC TACAGGAGGG CCAAGAGTGA AATGAGTGAG 
GGCCGGCAGG GCCGAGGCAG CTCCACGGGG GACGAGGAGG AGAGCCTGGC CATCCTGCGG 
AGGCACGTCA TGAACGAGCT CCTTGACACG GAACGGGTCT ACGTAGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCTGC CGAGATGGAC AACCCGTTAA TGACACATCT CATCTCAACA 
GGCCTTCAAA ACAAGAAGGA TGTTCTATTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGAATAT TCCTGAGGGA GCTAGAAAAC TACATAGACT GCCCAGAGCT GGTCGGAAGG 
TGTTTTCTAG AAAGGCTGGA GGAGTTCCAG ATGTACGAGA AGTACTGTCA GAACAAGCCG 
CGTTCCGAGA GCGTGTGGCG GCAGTGCGCC GACTGCCCCT TCTTCCAGGA ATGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA AGAGCTGTGA GGGGGCCGAG 
GACTTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CCATCACGGG CTATGACGGG AATCTGAGCG ACCTGGGGAA GCTGCTCATG 
CAGGGCTCCT TCAGCGTCTG GACGGACCAC AAGAAGGGCC ACAGCAAGGT GAAGGACCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCGGT GCTCTTCTGC 
AAGAAGAGAG AGGAGAACGG GGAGGGCTAT GAGAAAGCGC CTTCCTACAG CTACAAGCAG 
TCCCTGAACA TGGCGGCGGT TGGCATAACA GAGAACGTGA AGGGCGACGT GAGGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTT TACATCATAC AGGCACCAAC TCCCGAAATC 
AAAGCGGCGT GGGTGAGCGA AATCCGGAAG CTGCTGACCA GCCAGCTGCA GGCCTTCAGA 
GAAGCCAGCC AGCACCGCGT ACTGGAGCAG TCCCAGAGCC TGCCTCTGCC TGCAACGGCC 
AGCGCAAGTC CCTCGAAAGG GAACACAAGA AACGTCAAAA AGCTGGAAGA AAGGAAGACC 
GACCCCCTCA GCCTGGAAGG ATACGTGAGC TCGACATCGC TGCCAAAGGC CCCCGAGAAG 
GGCAGAGATG ACACGGTCAC TAACTCTACC TCAGAAAGCT CTGCACTTTC CAAAAAGCGC 
TTTACCCTGC AGGGTTTTGC TAACCTCAAA GGTCAGAAAG CTTCTCCCAC CAGTCCTGAC 
AAAAAAGCTA AACGGCACGA AGTAAAGAGC GACCCCACGC CTTTTGGTTT ACGAGGTTGG 
AGCAAAACGT CTCAGCCCTC CGAGGCCCCT GAGGACAACG ACGGCTGGTC GAGTGCTGAG 
GAGCCCCTTA ATTCCTCGGA CGCAGAGGAA GAAGGCGGAG TGGGCCCCAG GAAGCTGGTC 
CCGGGGAGGT TCACAGTTGT GGCGGATGAC GAGAAGGGCA GTCCTGACAC GCTGGTCATG 
AGGAGCGGGG ACCTGGTGGA GGTGGTCCAG GAGGGCGAGG ACTGCCTCTG GTATGTCAGG 
AACCTGACCT CCAGCAAGGA GGGCTGGGTG CCGGCCGGCA GCCTGTCCGC GCGCCTCGGC 
AAGTCCAGCT CAGCTCAGTG CTTGAGCAGC TCAGAGTCCA GCGCAGGGTC CACCCTGCTG 
AGCAGCTCCT CCAGCTGCAG TGAGAGCTGC GGGGTCCCCC TCTCAGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621872.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477640.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHb52018
Clone ID Related Accession (Same CDS sequence)	XM_023621872.1
Accession Version	XM_023621872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGGC GCGGGGAGGG CGCGCCGGGC TGCTGCGGCC TCTGCACCTG CCCAGGGGCG 
GCCGGAGATG AAATCATGCA CCAGGACATC ACCCCGCTCT GCGCCGCCGA CATCCAGGAC 
CAGCTGAGGA AGCGCTTTGC CTATCTGTCC GGCGGGCGGG GGCAGGACGG CAGCCCCGTC 
ATCACCTTCC CGGACTACCC GGCCTTCAGC GACGTCCCAG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGCTTA CAAGATGCTG GCATTGGGTT CATCCTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCG GTGAAGGCAT CAGTCCTGCG AATAGCAGCG 
TCCTTCCCGG CAAACCTCCA GCGCGTCCTT GTCCTGCGCC CAACGGGATT TTTCCAGAGG 
ACCCTCTCTG ACCTCGCTTT CAGATTCAAC AGAGATGACT TTAAGATGAA GGTACCGGTC 
ACAATGCTGA GCTCAGTACC AGAATTGCAC GATTACATTG ACAAGTCCCA GCTGACGGAG 
GACCTGGGCG GGACCCTGGA TTACTGCCAC ACCAAGTGGT TGTGTCACCG CACAGCCATT 
GAGAGTTTTG CTGTCATGGT GAAGCAGACG GCTCAGATGC TGCAGTCCTT CGGGACAGAG 
CTGGCCGAGA CGGAACTGCC CAACGACGTC CCATCCACAA GCTCTGTGCT CATCGCACAC 
ACGGAGAAGA AGCACAAAGC AAAGGAGAAC ATGAGGCTGG CGCTGGACGA GGGGCGGAGC 
ATCCTGGAGA GCATCAGGGA GCCTCTGGCC AGGAGCACGG AGCAGAGCCC GAACCAGGAC 
CAGCTGGACA ATCAGACCAC CGTGCAGAGG CTCCTGGCCC AGCTGAATGA AACCGAGGCC 
GCCTTTGATG AGTTTTGGGC AAAGCATCAG CAAAAACTCG AACAGTGTCT GCAGCTCCGG 
CATTTTGAGC AGGATTTCCG AGAGGTCAAA GCCGCCTTGG ACACGTTGGC CCAGAAGATA 
GTGACCTTCA CCGACGTGGG GAACAGCCTG GCGCACGTGG AGCACCTCCT GAAGGATCTC 
ACGAGCTTCG AGGAGAAGTC CAGTGCAGCC GTGGAGCGGG CCCGCGCCTT GTCCCTGGAG 
GGTGAGCAGC TCATCGGCAA CAAGCACTAT GCGGTGGACT CCATCCGCCC CAAGTGCCAC 
GAGCTGCAGC ACCTCTGCGA CCAGTTTGCC GCCGAGGTCG GGCGGAGGAG GGGGCTGCTC 
GGCAAGTCGC TGGAGCTGCA CAGCCTCCTG GAGGCGTCCA TGAAGTGGTG CGATGAAGGC 
ATCTACCTGC TGGCCTCCCA GCCTGTGGAC AAGTGCCAGT CCCAGGACGG CGCAGAGGCA 
GCCCTCCAGG AGATCGAGAA GTTTCTGGAG ACCGGCGCAG AAAATAAGAT CCAGGAGCTC 
AATAAAATTT ACCAGGACTA CGAACTCATC CTCACCCAAG ACCTGAGGGA ACACGTGCAG 
AAGGTCTTCC AGAAGCAAGA GAGCATGGAA GAGATGTTCC ACCGGAGGCA GGCGAGCCTG 
AAGAAGCTGG CGGCCAAGCA GACGCGGCCC GTGCAGCCGG TGGCCCCCCG GCCCGAGGCG 
CTCGCCAAGT CGCCCTGCGC CTCCCCAGGT ATCCGGAGAG GTTCTGAGAA CAGCAGTTGC 
GAGGGCAGCG CGCAGAGAGG GCCCTACAGG AGGGCCAAGA GTGAAATGAG TGAGGGCCGG 
CAGGGCCGAG GCAGCTCCAC GGGGGACGAG GAGGAGAGCC TGGCCATCCT GCGGAGGCAC 
GTCATGAACG AGCTCCTTGA CACGGAACGG GTCTACGTAG AGGAGCTGCT GTGCGTCCTG 
GAGGGCTACG CTGCCGAGAT GGACAACCCG TTAATGACAC ATCTCATCTC AACAGGCCTT 
CAAAACAAGA AGGATGTTCT ATTTGGAAAC ATGGAAGAAA TTTACCACTT TCATAACAGA 
ATATTCCTGA GGGAGCTAGA AAACTACATA GACTGCCCAG AGCTGGTCGG AAGGTGTTTT 
CTAGAAAGGC TGGAGGAGTT CCAGATGTAC GAGAAGTACT GTCAGAACAA GCCGCGTTCC 
GAGAGCGTGT GGCGGCAGTG CGCCGACTGC CCCTTCTTCC AGGAATGCCA GAAGAAGCTG 
GACCACAAGC TGAGCCTGGA CTCCTACCTG CTGAAGCCAG TCCAGAGGAT CACCAAGTAC 
CAGCTGCTGC TCAAGGAAAT GCTGAAATAC AGCAAGAGCT GTGAGGGGGC CGAGGACTTG 
CAGGAGGCGC TGAGCTCCAT CCTGGGCATC CTCAAGGCCG TGAACGACTC CATGCACCTC 
ATCGCCATCA CGGGCTATGA CGGGAATCTG AGCGACCTGG GGAAGCTGCT CATGCAGGGC 
TCCTTCAGCG TCTGGACGGA CCACAAGAAG GGCCACAGCA AGGTGAAGGA CCTGGCCAGG 
TTCAAGCCCA TGCAGCGGCA CCTGTTCCTG CACGAGAAGG CGGTGCTCTT CTGCAAGAAG 
AGAGAGGAGA ACGGGGAGGG CTATGAGAAA GCGCCTTCCT ACAGCTACAA GCAGTCCCTG 
AACATGGCGG CGGTTGGCAT AACAGAGAAC GTGAAGGGCG ACGTGAGGAA GTTTGAGATC 
TGGTACAACG CCCGGGAGGA GGTTTACATC ATACAGGCAC CAACTCCCGA AATCAAAGCG 
GCGTGGGTGA GCGAAATCCG GAAGCTGCTG ACCAGCCAGC TGCAGGCCTT CAGAGAAGCC 
AGCCAGCACC GCGTACTGGA GCAGTCCCAG AGCCTGCCTC TGCCTGCAAC GGCCAGCGCA 
AGTCCCTCGA AAGGGAACAC AAGAAACGTC AAAAAGCTGG AAGAAAGGAA GACCGACCCC 
CTCAGCCTGG AAGGATACGT GAGCTCGACA TCGCTGCCAA AGGCCCCCGA GAAGGGCAGA 
GATGACACGG TCACTAACTC TACCTCAGAA AGCTCTGCAC TTTCCAAAAA GCGCTTTACC 
CTGCAGGGTT TTGCTAACCT CAAAGGTCAG AAAGCTTCTC CCACCAGTCC TGACAAAAAA 
GCTAAACGGC ACGAAGTAAA GAGCGACCCC ACGCCTTTTG GTTTACGAGG TTGGAGCAAA 
ACGTCTCAGC CCTCCGAGGC CCCTGAGGAC AACGACGGCT GGTCGAGTGC TGAGGAGCCC 
CTTAATTCCT CGGACGCAGA GGAAGAAGGC GGAGTGGGCC CCAGGAAGCT GGTCCCGGGG 
AGGTTCACAG TTGTGGCGGA TGACGAGAAG GGCAGTCCTG ACACGCTGGT CATGAGGAGC 
GGGGACCTGG TGGAGGTGGT CCAGGAGGGC GAGGACTGCC TCTGGTATGT CAGGAACCTG 
ACCTCCAGCA AGGAGGGCTG GGTGCCGGCC GGCAGCCTGT CCGCGCGCCT CGGCAAGTCC 
AGCTCAGCTC AGTGCTTGAG CAGCTCAGAG TCCAGCGCAG GGTCCACCCT GCTGAGCAGC 
TCCTCCAGCT GCAGTGAGAG CTGCGGGGTC CCCCTCTCAG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477640.1
CDS	653..4186
Translation	MRRRGEGAPGCCGLCTCPGAAGDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621872.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621872.1

ORF Insert Sequence:

ATGCGGCGGC GCGGGGAGGG CGCGCCGGGC TGCTGCGGCC TCTGCACCTG CCCAGGGGCG 
GCCGGAGATG AAATCATGCA CCAGGACATC ACCCCGCTCT GCGCCGCCGA CATCCAGGAC 
CAGCTGAGGA AGCGCTTTGC CTATCTGTCC GGCGGGCGGG GGCAGGACGG CAGCCCCGTC 
ATCACCTTCC CGGACTACCC GGCCTTCAGC GACGTCCCAG ACAAGGAGTT CCAGAATGTC 
ATGACCTATC TCACCAGCAT CCCCAGCTTA CAAGATGCTG GCATTGGGTT CATCCTGGTC 
ATAGACCGAA GGCAGGACAA ATGGACCTCG GTGAAGGCAT CAGTCCTGCG AATAGCAGCG 
TCCTTCCCGG CAAACCTCCA GCGCGTCCTT GTCCTGCGCC CAACGGGATT TTTCCAGAGG 
ACCCTCTCTG ACCTCGCTTT CAGATTCAAC AGAGATGACT TTAAGATGAA GGTACCGGTC 
ACAATGCTGA GCTCAGTACC AGAATTGCAC GATTACATTG ACAAGTCCCA GCTGACGGAG 
GACCTGGGCG GGACCCTGGA TTACTGCCAC ACCAAGTGGT TGTGTCACCG CACAGCCATT 
GAGAGTTTTG CTGTCATGGT GAAGCAGACG GCTCAGATGC TGCAGTCCTT CGGGACAGAG 
CTGGCCGAGA CGGAACTGCC CAACGACGTC CCATCCACAA GCTCTGTGCT CATCGCACAC 
ACGGAGAAGA AGCACAAAGC AAAGGAGAAC ATGAGGCTGG CGCTGGACGA GGGGCGGAGC 
ATCCTGGAGA GCATCAGGGA GCCTCTGGCC AGGAGCACGG AGCAGAGCCC GAACCAGGAC 
CAGCTGGACA ATCAGACCAC CGTGCAGAGG CTCCTGGCCC AGCTGAATGA AACCGAGGCC 
GCCTTTGATG AGTTTTGGGC AAAGCATCAG CAAAAACTCG AACAGTGTCT GCAGCTCCGG 
CATTTTGAGC AGGATTTCCG AGAGGTCAAA GCCGCCTTGG ACACGTTGGC CCAGAAGATA 
GTGACCTTCA CCGACGTGGG GAACAGCCTG GCGCACGTGG AGCACCTCCT GAAGGATCTC 
ACGAGCTTCG AGGAGAAGTC CAGTGCAGCC GTGGAGCGGG CCCGCGCCTT GTCCCTGGAG 
GGTGAGCAGC TCATCGGCAA CAAGCACTAT GCGGTGGACT CCATCCGCCC CAAGTGCCAC 
GAGCTGCAGC ACCTCTGCGA CCAGTTTGCC GCCGAGGTCG GGCGGAGGAG GGGGCTGCTC 
GGCAAGTCGC TGGAGCTGCA CAGCCTCCTG GAGGCGTCCA TGAAGTGGTG CGATGAAGGC 
ATCTACCTGC TGGCCTCCCA GCCTGTGGAC AAGTGCCAGT CCCAGGACGG CGCAGAGGCA 
GCCCTCCAGG AGATCGAGAA GTTTCTGGAG ACCGGCGCAG AAAATAAGAT CCAGGAGCTC 
AATAAAATTT ACCAGGACTA CGAACTCATC CTCACCCAAG ACCTGAGGGA ACACGTGCAG 
AAGGTCTTCC AGAAGCAAGA GAGCATGGAA GAGATGTTCC ACCGGAGGCA GGCGAGCCTG 
AAGAAGCTGG CGGCCAAGCA GACGCGGCCC GTGCAGCCGG TGGCCCCCCG GCCCGAGGCG 
CTCGCCAAGT CGCCCTGCGC CTCCCCAGGT ATCCGGAGAG GTTCTGAGAA CAGCAGTTGC 
GAGGGCAGCG CGCAGAGAGG GCCCTACAGG AGGGCCAAGA GTGAAATGAG TGAGGGCCGG 
CAGGGCCGAG GCAGCTCCAC GGGGGACGAG GAGGAGAGCC TGGCCATCCT GCGGAGGCAC 
GTCATGAACG AGCTCCTTGA CACGGAACGG GTCTACGTAG AGGAGCTGCT GTGCGTCCTG 
GAGGGCTACG CTGCCGAGAT GGACAACCCG TTAATGACAC ATCTCATCTC AACAGGCCTT 
CAAAACAAGA AGGATGTTCT ATTTGGAAAC ATGGAAGAAA TTTACCACTT TCATAACAGA 
ATATTCCTGA GGGAGCTAGA AAACTACATA GACTGCCCAG AGCTGGTCGG AAGGTGTTTT 
CTAGAAAGGC TGGAGGAGTT CCAGATGTAC GAGAAGTACT GTCAGAACAA GCCGCGTTCC 
GAGAGCGTGT GGCGGCAGTG CGCCGACTGC CCCTTCTTCC AGGAATGCCA GAAGAAGCTG 
GACCACAAGC TGAGCCTGGA CTCCTACCTG CTGAAGCCAG TCCAGAGGAT CACCAAGTAC 
CAGCTGCTGC TCAAGGAAAT GCTGAAATAC AGCAAGAGCT GTGAGGGGGC CGAGGACTTG 
CAGGAGGCGC TGAGCTCCAT CCTGGGCATC CTCAAGGCCG TGAACGACTC CATGCACCTC 
ATCGCCATCA CGGGCTATGA CGGGAATCTG AGCGACCTGG GGAAGCTGCT CATGCAGGGC 
TCCTTCAGCG TCTGGACGGA CCACAAGAAG GGCCACAGCA AGGTGAAGGA CCTGGCCAGG 
TTCAAGCCCA TGCAGCGGCA CCTGTTCCTG CACGAGAAGG CGGTGCTCTT CTGCAAGAAG 
AGAGAGGAGA ACGGGGAGGG CTATGAGAAA GCGCCTTCCT ACAGCTACAA GCAGTCCCTG 
AACATGGCGG CGGTTGGCAT AACAGAGAAC GTGAAGGGCG ACGTGAGGAA GTTTGAGATC 
TGGTACAACG CCCGGGAGGA GGTTTACATC ATACAGGCAC CAACTCCCGA AATCAAAGCG 
GCGTGGGTGA GCGAAATCCG GAAGCTGCTG ACCAGCCAGC TGCAGGCCTT CAGAGAAGCC 
AGCCAGCACC GCGTACTGGA GCAGTCCCAG AGCCTGCCTC TGCCTGCAAC GGCCAGCGCA 
AGTCCCTCGA AAGGGAACAC AAGAAACGTC AAAAAGCTGG AAGAAAGGAA GACCGACCCC 
CTCAGCCTGG AAGGATACGT GAGCTCGACA TCGCTGCCAA AGGCCCCCGA GAAGGGCAGA 
GATGACACGG TCACTAACTC TACCTCAGAA AGCTCTGCAC TTTCCAAAAA GCGCTTTACC 
CTGCAGGGTT TTGCTAACCT CAAAGGTCAG AAAGCTTCTC CCACCAGTCC TGACAAAAAA 
GCTAAACGGC ACGAAGTAAA GAGCGACCCC ACGCCTTTTG GTTTACGAGG TTGGAGCAAA 
ACGTCTCAGC CCTCCGAGGC CCCTGAGGAC AACGACGGCT GGTCGAGTGC TGAGGAGCCC 
CTTAATTCCT CGGACGCAGA GGAAGAAGGC GGAGTGGGCC CCAGGAAGCT GGTCCCGGGG 
AGGTTCACAG TTGTGGCGGA TGACGAGAAG GGCAGTCCTG ACACGCTGGT CATGAGGAGC 
GGGGACCTGG TGGAGGTGGT CCAGGAGGGC GAGGACTGCC TCTGGTATGT CAGGAACCTG 
ACCTCCAGCA AGGAGGGCTG GGTGCCGGCC GGCAGCCTGT CCGCGCGCCT CGGCAAGTCC 
AGCTCAGCTC AGTGCTTGAG CAGCTCAGAG TCCAGCGCAG GGTCCACCCT GCTGAGCAGC 
TCCTCCAGCT GCAGTGAGAG CTGCGGGGTC CCCCTCTCAG ACCTGCAGGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_014732206.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587692.2 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X15, mRNA.

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CloneID	OHb52019
Clone ID Related Accession (Same CDS sequence)	XM_014732206.2
Accession Version	XM_014732206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014732206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTCAC TTGTTCTGCA CCTCTCAGAT GAAATCATGC ACCAGGACAT CACCCCGCTC 
TGCGCCGCCG ACATCCAGGA CCAGCTGAGG AAGCGCTTTG CCTATCTGTC CGGCGGGCGG 
GGGCAGGACG GCAGCCCCGT CATCACCTTC CCGGACTACC CGGCCTTCAG CGACGTCCCA 
GACAAGGAGT TCCAGAATGT CATGACCTAT CTCACCAGCA TCCCCAGCTT ACAAGATGCT 
GGCATTGGGT TCATCCTGGT CATAGACCGA AGGCAGGACA AATGGACCTC GGTGAAGGCA 
TCAGTCCTGC GAATAGCAGC GTCCTTCCCG GCAAACCTCC AGCGCGTCCT TGTCCTGCGC 
CCAACGGGAT TTTTCCAGAG GACCCTCTCT GACCTCGCTT TCAGATTCAA CAGAGATGAC 
TTTAAGATGA AGGTACCGGT CACAATGCTG AGCTCAGTAC CAGAATTGCA CGATTACATT 
GACAAGTCCC AGCTGACGGA GGACCTGGGC GGGACCCTGG ATTACTGCCA CACCAAGTGG 
TTGTGTCACC GCACAGCCAT TGAGAGTTTT GCTGTCATGG TGAAGCAGAC GGCTCAGATG 
CTGCAGTCCT TCGGGACAGA GCTGGCCGAG ACGGAACTGC CCAACGACGT CCCATCCACA 
AGCTCTGTGC TCATCGCACA CACGGAGAAG AAGCACAAAG CAAAGGAGAA CATGAGGCTG 
GCGCTGGACG AGGGGCGGAG CATCCTGGAG AGCATCAGGG AGCCTCTGGC CAGGAGCACG 
GAGCAGAGCC CGAACCAGGA CCAGCTGGAC AATCAGACCA CCGTGCAGAG GCTCCTGGCC 
CAGCTGAATG AAACCGAGGC CGCCTTTGAT GAGTTTTGGG CAAAGCATCA GCAAAAACTC 
GAACAGTGTC TGCAGCTCCG GCATTTTGAG CAGGATTTCC GAGAGGTCAA AGCCGCCTTG 
GACACGTTGG CCCAGAAGAT AGTGACCTTC ACCGACGTGG GGAACAGCCT GGCGCACGTG 
GAGCACCTCC TGAAGGATCT CACGAGCTTC GAGGAGAAGT CCAGTGCAGC CGTGGAGCGG 
GCCCGCGCCT TGTCCCTGGA GGGTGAGCAG CTCATCGGCA ACAAGCACTA TGCGGTGGAC 
TCCATCCGCC CCAAGTGCCA CGAGCTGCAG CACCTCTGCG ACCAGTTTGC CGCCGAGGTC 
GGGCGGAGGA GGGGGCTGCT CGGCAAGTCG CTGGAGCTGC ACAGCCTCCT GGAGGCGTCC 
ATGAAGTGGT GCGATGAAGG CATCTACCTG CTGGCCTCCC AGCCTGTGGA CAAGTGCCAG 
TCCCAGGACG GCGCAGAGGC AGCCCTCCAG GAGATCGAGA AGTTTCTGGA GACCGGCGCA 
GAAAATAAGA TCCAGGAGCT CAATAAAATT TACCAGGACT ACGAACTCAT CCTCACCCAA 
GACCTGAGGG AACACGTGCA GAAGGTCTTC CAGAAGCAAG AGAGCATGGA AGAGATGTTC 
CACCGGAGGC AGGCGAGCCT GAAGAAGCTG GCGGCCAAGC AGACGCGGCC CGTGCAGCCG 
GTGGCCCCCC GGCCCGAGGC GCTCGCCAAG TCGCCCTGCG CCTCCCCAGG TATCCGGAGA 
GGTTCTGAGA ACAGCAGTTG CGAGGGCAGC GCGCAGAGAG GGCCCTACAG GAGGGCCAAG 
AGTGAAATGA GTGAGGGCCG GCAGGGCCGA GGCAGCTCCA CGGGGGACGA GGAGGAGAGC 
CTGGCCATCC TGCGGAGGCA CGTCATGAAC GAGCTCCTTG ACACGGAACG GGTCTACGTA 
GAGGAGCTGC TGTGCGTCCT GGAGGGCTAC GCTGCCGAGA TGGACAACCC GTTAATGACA 
CATCTCATCT CAACAGGCCT TCAAAACAAG AAGGATGTTC TATTTGGAAA CATGGAAGAA 
ATTTACCACT TTCATAACAG AATATTCCTG AGGGAGCTAG AAAACTACAT AGACTGCCCA 
GAGCTGGTCG GAAGGTGTTT TCTAGAAAGG CTGGAGGAGT TCCAGATGTA CGAGAAGTAC 
TGTCAGAACA AGCCGCGTTC CGAGAGCGTG TGGCGGCAGT GCGCCGACTG CCCCTTCTTC 
CAGGAATGCC AGAAGAAGCT GGACCACAAG CTGAGCCTGG ACTCCTACCT GCTGAAGCCA 
GTCCAGAGGA TCACCAAGTA CCAGCTGCTG CTCAAGGAAA TGCTGAAATA CAGCAAGAGC 
TGTGAGGGGG CCGAGGACTT GCAGGAGGCG CTGAGCTCCA TCCTGGGCAT CCTCAAGGCC 
GTGAACGACT CCATGCACCT CATCGCCATC ACGGGCTATG ACGGGAATCT GAGCGACCTG 
GGGAAGCTGC TCATGCAGGG CTCCTTCAGC GTCTGGACGG ACCACAAGAA GGGCCACAGC 
AAGGTGAAGG ACCTGGCCAG GTTCAAGCCC ATGCAGCGGC ACCTGTTCCT GCACGAGAAG 
GCGGTGCTCT TCTGCAAGAA GAGAGAGGAG AACGGGGAGG GCTATGAGAA AGCGCCTTCC 
TACAGCTACA AGCAGTCCCT GAACATGGCG GCGGTTGGCA TAACAGAGAA CGTGAAGGGC 
GACGTGAGGA AGTTTGAGAT CTGGTACAAC GCCCGGGAGG AGGTTTACAT CATACAGGCA 
CCAACTCCCG AAATCAAAGC GGCGTGGGTG AGCGAAATCC GGAAGCTGCT GACCAGCCAG 
CTGCAGGCCT TCAGAGAAGC CAGCCAGCAC CGCGTACTGG AGCAGTCCCA GAGCCTGCCT 
CTGCCTGCAA CGGCCAGCGC AAGTCCCTCG AAAGGGAACA CAAGAAACGT CAAAAAGCTG 
GAAGAAAGGA AGACCGACCC CCTCAGCCTG GAAGGATACG TGAGCTCGAC ATCGCTGCCA 
AAGGCCCCCG AGAAGGGCAG AGATGACACG GTCACTAACT CTACCTCAGA AAGCTCTGCA 
CTTTCCAAAA AGCGCTTTAC CCTGCAGGGT TTTGCTAACC TCAAAGGTCA GAAAGCTTCT 
CCCACCAGTC CTGACAAAAA AGCTAAACGG CACGAAGTAA AGAGCGACCC CACGCCTTTT 
GGTTTACGAG GTTGGAGCAA AACGTCTCAG CCCTCCGAGG CCCCTGAGGA CAACGACGGC 
TGGTCGAGTG CTGAGGAGCC CCTTAATTCC TCGGACGCAG AGGAAGAAGG CGGAGTGGGC 
CCCAGGAAGC TGGTCCCGGG GAGGTTCACA GTTGTGGCGG ATGACGAGAA GGGCAGTCCT 
GACACGCTGG TCATGAGGAG CGGGGACCTG GTGGAGGTGG TCCAGGAGGG CGAGGACTGC 
CTCTGGTATG TCAGGAACCT GACCTCCAGC AAGGAGGGCT GGGTGCCGGC CGGCAGCCTG 
TCCGCGCGCC TCGGCAAGTC CAGCTCAGCT CAGTGCTTGA GCAGCTCAGA GTCCAGCGCA 
GGGTCCACCC TGCTGAGCAG CTCCTCCAGC TGCAGTGAGA GCTGCGGGGT CCCCCTCTCA 
GACCTGCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587692.2
CDS	2212..5706
Translation	MRSLVLHLSDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732206.2
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732206.2

ORF Insert Sequence:

ATGAGGTCAC TTGTTCTGCA CCTCTCAGAT GAAATCATGC ACCAGGACAT CACCCCGCTC 
TGCGCCGCCG ACATCCAGGA CCAGCTGAGG AAGCGCTTTG CCTATCTGTC CGGCGGGCGG 
GGGCAGGACG GCAGCCCCGT CATCACCTTC CCGGACTACC CGGCCTTCAG CGACGTCCCA 
GACAAGGAGT TCCAGAATGT CATGACCTAT CTCACCAGCA TCCCCAGCTT ACAAGATGCT 
GGCATTGGGT TCATCCTGGT CATAGACCGA AGGCAGGACA AATGGACCTC GGTGAAGGCA 
TCAGTCCTGC GAATAGCAGC GTCCTTCCCG GCAAACCTCC AGCGCGTCCT TGTCCTGCGC 
CCAACGGGAT TTTTCCAGAG GACCCTCTCT GACCTCGCTT TCAGATTCAA CAGAGATGAC 
TTTAAGATGA AGGTACCGGT CACAATGCTG AGCTCAGTAC CAGAATTGCA CGATTACATT 
GACAAGTCCC AGCTGACGGA GGACCTGGGC GGGACCCTGG ATTACTGCCA CACCAAGTGG 
TTGTGTCACC GCACAGCCAT TGAGAGTTTT GCTGTCATGG TGAAGCAGAC GGCTCAGATG 
CTGCAGTCCT TCGGGACAGA GCTGGCCGAG ACGGAACTGC CCAACGACGT CCCATCCACA 
AGCTCTGTGC TCATCGCACA CACGGAGAAG AAGCACAAAG CAAAGGAGAA CATGAGGCTG 
GCGCTGGACG AGGGGCGGAG CATCCTGGAG AGCATCAGGG AGCCTCTGGC CAGGAGCACG 
GAGCAGAGCC CGAACCAGGA CCAGCTGGAC AATCAGACCA CCGTGCAGAG GCTCCTGGCC 
CAGCTGAATG AAACCGAGGC CGCCTTTGAT GAGTTTTGGG CAAAGCATCA GCAAAAACTC 
GAACAGTGTC TGCAGCTCCG GCATTTTGAG CAGGATTTCC GAGAGGTCAA AGCCGCCTTG 
GACACGTTGG CCCAGAAGAT AGTGACCTTC ACCGACGTGG GGAACAGCCT GGCGCACGTG 
GAGCACCTCC TGAAGGATCT CACGAGCTTC GAGGAGAAGT CCAGTGCAGC CGTGGAGCGG 
GCCCGCGCCT TGTCCCTGGA GGGTGAGCAG CTCATCGGCA ACAAGCACTA TGCGGTGGAC 
TCCATCCGCC CCAAGTGCCA CGAGCTGCAG CACCTCTGCG ACCAGTTTGC CGCCGAGGTC 
GGGCGGAGGA GGGGGCTGCT CGGCAAGTCG CTGGAGCTGC ACAGCCTCCT GGAGGCGTCC 
ATGAAGTGGT GCGATGAAGG CATCTACCTG CTGGCCTCCC AGCCTGTGGA CAAGTGCCAG 
TCCCAGGACG GCGCAGAGGC AGCCCTCCAG GAGATCGAGA AGTTTCTGGA GACCGGCGCA 
GAAAATAAGA TCCAGGAGCT CAATAAAATT TACCAGGACT ACGAACTCAT CCTCACCCAA 
GACCTGAGGG AACACGTGCA GAAGGTCTTC CAGAAGCAAG AGAGCATGGA AGAGATGTTC 
CACCGGAGGC AGGCGAGCCT GAAGAAGCTG GCGGCCAAGC AGACGCGGCC CGTGCAGCCG 
GTGGCCCCCC GGCCCGAGGC GCTCGCCAAG TCGCCCTGCG CCTCCCCAGG TATCCGGAGA 
GGTTCTGAGA ACAGCAGTTG CGAGGGCAGC GCGCAGAGAG GGCCCTACAG GAGGGCCAAG 
AGTGAAATGA GTGAGGGCCG GCAGGGCCGA GGCAGCTCCA CGGGGGACGA GGAGGAGAGC 
CTGGCCATCC TGCGGAGGCA CGTCATGAAC GAGCTCCTTG ACACGGAACG GGTCTACGTA 
GAGGAGCTGC TGTGCGTCCT GGAGGGCTAC GCTGCCGAGA TGGACAACCC GTTAATGACA 
CATCTCATCT CAACAGGCCT TCAAAACAAG AAGGATGTTC TATTTGGAAA CATGGAAGAA 
ATTTACCACT TTCATAACAG AATATTCCTG AGGGAGCTAG AAAACTACAT AGACTGCCCA 
GAGCTGGTCG GAAGGTGTTT TCTAGAAAGG CTGGAGGAGT TCCAGATGTA CGAGAAGTAC 
TGTCAGAACA AGCCGCGTTC CGAGAGCGTG TGGCGGCAGT GCGCCGACTG CCCCTTCTTC 
CAGGAATGCC AGAAGAAGCT GGACCACAAG CTGAGCCTGG ACTCCTACCT GCTGAAGCCA 
GTCCAGAGGA TCACCAAGTA CCAGCTGCTG CTCAAGGAAA TGCTGAAATA CAGCAAGAGC 
TGTGAGGGGG CCGAGGACTT GCAGGAGGCG CTGAGCTCCA TCCTGGGCAT CCTCAAGGCC 
GTGAACGACT CCATGCACCT CATCGCCATC ACGGGCTATG ACGGGAATCT GAGCGACCTG 
GGGAAGCTGC TCATGCAGGG CTCCTTCAGC GTCTGGACGG ACCACAAGAA GGGCCACAGC 
AAGGTGAAGG ACCTGGCCAG GTTCAAGCCC ATGCAGCGGC ACCTGTTCCT GCACGAGAAG 
GCGGTGCTCT TCTGCAAGAA GAGAGAGGAG AACGGGGAGG GCTATGAGAA AGCGCCTTCC 
TACAGCTACA AGCAGTCCCT GAACATGGCG GCGGTTGGCA TAACAGAGAA CGTGAAGGGC 
GACGTGAGGA AGTTTGAGAT CTGGTACAAC GCCCGGGAGG AGGTTTACAT CATACAGGCA 
CCAACTCCCG AAATCAAAGC GGCGTGGGTG AGCGAAATCC GGAAGCTGCT GACCAGCCAG 
CTGCAGGCCT TCAGAGAAGC CAGCCAGCAC CGCGTACTGG AGCAGTCCCA GAGCCTGCCT 
CTGCCTGCAA CGGCCAGCGC AAGTCCCTCG AAAGGGAACA CAAGAAACGT CAAAAAGCTG 
GAAGAAAGGA AGACCGACCC CCTCAGCCTG GAAGGATACG TGAGCTCGAC ATCGCTGCCA 
AAGGCCCCCG AGAAGGGCAG AGATGACACG GTCACTAACT CTACCTCAGA AAGCTCTGCA 
CTTTCCAAAA AGCGCTTTAC CCTGCAGGGT TTTGCTAACC TCAAAGGTCA GAAAGCTTCT 
CCCACCAGTC CTGACAAAAA AGCTAAACGG CACGAAGTAA AGAGCGACCC CACGCCTTTT 
GGTTTACGAG GTTGGAGCAA AACGTCTCAG CCCTCCGAGG CCCCTGAGGA CAACGACGGC 
TGGTCGAGTG CTGAGGAGCC CCTTAATTCC TCGGACGCAG AGGAAGAAGG CGGAGTGGGC 
CCCAGGAAGC TGGTCCCGGG GAGGTTCACA GTTGTGGCGG ATGACGAGAA GGGCAGTCCT 
GACACGCTGG TCATGAGGAG CGGGGACCTG GTGGAGGTGG TCCAGGAGGG CGAGGACTGC 
CTCTGGTATG TCAGGAACCT GACCTCCAGC AAGGAGGGCT GGGTGCCGGC CGGCAGCCTG 
TCCGCGCGCC TCGGCAAGTC CAGCTCAGCT CAGTGCTTGA GCAGCTCAGA GTCCAGCGCA 
GGGTCCACCC TGCTGAGCAG CTCCTCCAGC TGCAGTGAGA GCTGCGGGGT CCCCCTCTCA 
GACCTGCAGG GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_023621873.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023477641.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X17, mRNA.

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CloneID	OHb52020
Clone ID Related Accession (Same CDS sequence)	XM_023621873.1 , XM_014732209.2
Accession Version	XM_023621873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCCGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCAGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAACGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CGGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACACG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023477641.1
CDS	385..3843
Translation	MHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_023621873.1
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023621873.1

ORF Insert Sequence:

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCCGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCAGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAACGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CGGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACACG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_014732209.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014587695.2 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

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CloneID	OHb52020
Clone ID Related Accession (Same CDS sequence)	XM_023621873.1 , XM_014732209.2
Accession Version	XM_014732209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014732209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCCGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCAGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAACGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CGGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACACG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014587695.2
CDS	161..3619
Translation	MHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_014732209.2
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014732209.2

ORF Insert Sequence:

ATGCACCAGG ACATCACCCC GCTCTGCGCC GCCGACATCC AGGACCAGCT GAGGAAGCGC 
TTTGCCTATC TGTCCGGCGG GCGGGGGCAG GACGGCAGCC CCGTCATCAC CTTCCCGGAC 
TACCCGGCCT TCAGCGACGT CCCAGACAAG GAGTTCCAGA ATGTCATGAC CTATCTCACC 
AGCATCCCCA GCTTACAAGA TGCTGGCATT GGGTTCATCC TGGTCATAGA CCGAAGGCAG 
GACAAATGGA CCTCGGTGAA GGCATCAGTC CTGCGAATAG CAGCGTCCTT CCCGGCAAAC 
CTCCAGCGCG TCCTTGTCCT GCGCCCAACG GGATTTTTCC AGAGGACCCT CTCTGACCTC 
GCTTTCAGAT TCAACAGAGA TGACTTTAAG ATGAAGGTAC CGGTCACAAT GCTGAGCTCA 
GTACCAGAAT TGCACGATTA CATTGACAAG TCCCAGCTGA CGGAGGACCT GGGCGGGACC 
CTGGATTACT GCCACACCAA GTGGTTGTGT CACCGCACAG CCATTGAGAG TTTTGCTGTC 
ATGGTGAAGC AGACGGCTCA GATGCTGCAG TCCTTCGGGA CAGAGCTGGC CGAGACGGAA 
CTGCCCAACG ACGTCCCATC CACAAGCTCT GTGCTCATCG CACACACGGA GAAGAAGCAC 
AAAGCAAAGG AGAACATGAG GCTGGCGCTG GACGAGGGGC GGAGCATCCT GGAGAGCATC 
AGGGAGCCTC TGGCCAGGAG CACGGAGCAG AGCCCGAACC AGGACCAGCT GGACAATCAG 
ACCACCGTGC AGAGGCTCCT GGCCCAGCTG AATGAAACCG AGGCCGCCTT TGATGAGTTT 
TGGGCAAAGC ATCAGCAAAA ACTCGAACAG TGTCTGCAGC TCCGGCATTT TGAGCAGGAT 
TTCCGAGAGG TCAAAGCCGC CTTGGACACG TTGGCCCAGA AGATAGTGAC CTTCACCGAC 
GTGGGGAACA GCCTGGCGCA CGTGGAGCAC CTCCTGAAGG ATCTCACGAG CTTCGAGGAG 
AAGTCCAGTG CAGCCGTGGA GCGGGCCCGC GCCTTGTCCC TGGAGGGTGA GCAGCTCATC 
GGCAACAAGC ACTATGCGGT GGACTCCATC CGCCCCAAGT GCCACGAGCT GCAGCACCTC 
TGCGACCAGT TTGCCGCCGA GGTCGGGCGG AGGAGGGGGC TGCTCGGCAA GTCGCTGGAG 
CTGCACAGCC TCCTGGAGGC GTCCATGAAG TGGTGCGATG AAGGCATCTA CCTGCTGGCC 
TCCCAGCCTG TGGACAAGTG CCAGTCCCAG GACGGCGCAG AGGCAGCCCT CCAGGAGATC 
GAGAAGTTTC TGGAGACCGG CGCAGAAAAT AAGATCCAGG AGCTCAATAA AATTTACCAG 
GACTACGAAC TCATCCTCAC CCAAGACCTG AGGGAACACG TGCAGAAGGT CTTCCAGAAG 
CAAGAGAGCA TGGAAGAGAT GTTCCACCGG AGGCAGGCGA GCCTGAAGAA GCTGGCGGCC 
AAGCAGACGC GGCCCGTGCA GCCGGTGGCC CCCCGGCCCG AGGCGCTCGC CAAGTCGCCC 
TGCGCCTCCC CAGGTATCCG GAGAGGTTCT GAGAACAGCA GTTGCGAGGG CAGCGCGCAG 
AGAGGGCCCT ACAGGAGGGC CAAGAGTGAA ATGAGTGAGG GCCGGCAGGG CCGAGGCAGC 
TCCACGGGGG ACGAGGAGGA GAGCCTGGCC ATCCTGCGGA GGCACGTCAT GAACGAGCTC 
CTTGACACGG AACGGGTCTA CGTAGAGGAG CTGCTGTGCG TCCTGGAGGG CTACGCTGCC 
GAGATGGACA ACCCGTTAAT GACACATCTC ATCTCAACAG GCCTTCAAAA CAAGAAGGAT 
GTTCTATTTG GAAACATGGA AGAAATTTAC CACTTTCATA ACAGAATATT CCTGAGGGAG 
CTAGAAAACT ACATAGACTG CCCAGAGCTG GTCGGAAGGT GTTTTCTAGA AAGGCTGGAG 
GAGTTCCAGA TGTACGAGAA GTACTGTCAG AACAAGCCGC GTTCCGAGAG CGTGTGGCGG 
CAGTGCGCCG ACTGCCCCTT CTTCCAGGAA TGCCAGAAGA AGCTGGACCA CAAGCTGAGC 
CTGGACTCCT ACCTGCTGAA GCCAGTCCAG AGGATCACCA AGTACCAGCT GCTGCTCAAG 
GAAATGCTGA AATACAGCAA GAGCTGTGAG GGGGCCGAGG ACTTGCAGGA GGCGCTGAGC 
TCCATCCTGG GCATCCTCAA GGCCGTGAAC GACTCCATGC ACCTCATCGC CATCACGGGC 
TATGACGGGA ATCTGAGCGA CCTGGGGAAG CTGCTCATGC AGGGCTCCTT CAGCGTCTGG 
ACGGACCACA AGAAGGGCCA CAGCAAGGTG AAGGACCTGG CCAGGTTCAA GCCCATGCAG 
CGGCACCTGT TCCTGCACGA GAAGGCGGTG CTCTTCTGCA AGAAGAGAGA GGAGAACGGG 
GAGGGCTATG AGAAAGCGCC TTCCTACAGC TACAAGCAGT CCCTGAACAT GGCGGCGGTT 
GGCATAACAG AGAACGTGAA GGGCGACGTG AGGAAGTTTG AGATCTGGTA CAACGCCCGG 
GAGGAGGTTT ACATCATACA GGCACCAACT CCCGAAATCA AAGCGGCGTG GGTGAGCGAA 
ATCCGGAAGC TGCTGACCAG CCAGCTGCAG GCCTTCAGAG AAGCCAGCCA GCACCGCGTA 
CTGGAGCAGT CCCAGAGCCT GCCTCTGCCT GCAACGGCCA GCGCAAGTCC CTCGAAAGGG 
AACACAAGAA ACGTCAAAAA GCTGGAAGAA AGGAAGACCG ACCCCCTCAG CCTGGAAGGA 
TACGTGAGCT CGACATCGCT GCCAAAGGCC CCCGAGAAGG GCAGAGATGA CACGGTCACT 
AACTCTACCT CAGAAAGCTC TGCACTTTCC AAAAAGCGCT TTACCCTGCA GGGTTTTGCT 
AACCTCAAAG GTCAGAAAGC TTCTCCCACC AGTCCTGACA AAAAAGCTAA ACGGCACGAA 
GTAAAGAGCG ACCCCACGCC TTTTGGTTTA CGAGGTTGGA GCAAAACGTC TCAGCCCTCC 
GAGGCCCCTG AGGACAACGA CGGCTGGTCG AGTGCTGAGG AGCCCCTTAA TTCCTCGGAC 
GCAGAGGAAG AAGGCGGAGT GGGCCCCAGG AAGCTGGTCC CGGGGAGGTT CACAGTTGTG 
GCGGATGACG AGAAGGGCAG TCCTGACACG CTGGTCATGA GGAGCGGGGA CCTGGTGGAG 
GTGGTCCAGG AGGGCGAGGA CTGCCTCTGG TATGTCAGGA ACCTGACCTC CAGCAAGGAG 
GGCTGGGTGC CGGCCGGCAG CCTGTCCGCG CGCCTCGGCA AGTCCAGCTC AGCTCAGTGC 
TTGAGCAGCT CAGAGTCCAG CGCAGGGTCC ACCCTGCTGA GCAGCTCCTC CAGCTGCAGT 
GAGAGCTGCG GGGTCCCCCT CTCAGACCTG CAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_005601350.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601407.2 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X18, mRNA.

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CloneID	OHb52021
Clone ID Related Accession (Same CDS sequence)	XM_005601350.3
Accession Version	XM_005601350.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009160.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005601350.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCCGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CAGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAACGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC GGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACACGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601407.2
CDS	443..3820
Translation	MRFWLRNEEMALEEMVQRLNAVSARTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDTLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_005601350.3
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601350.3

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCCGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CAGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAACGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC GGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACACGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_005601347.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601404.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb08031
Clone ID Related Accession (Same CDS sequence)	XM_005601347.2
Accession Version	XM_005601347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005601347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCGGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CCGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAATGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGATGACAC GGTCACTAAC TCTACCTCAG AAAGCTCTGC ACTTTCCAAA 
AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC CTCAAAGGTC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAACG GCACGAAGTA AAGAGCGACC CCACGCCTTT TGGTTTACGA 
GGTTGGAGCA AAACGTCTCA GCCCTCCGAG GCCCCTGAGG ACAACGACGG CTGGTCGAGT 
GCTGAGGAGC CCCTTAATTC CTCGGACGCA GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG 
CTGGTCCCAG GGAGGTTCAC AGTTGTGGCG GATGACGAGA AGGGCAGTCC TGACATGCTG 
GTCATGAGGA GCGGGGACCT GGTGGAGGTG GTCCAGGAGG GCGAGGACTG CCTCTGGTAT 
GTCAGGAACC TGACCTCCAG CAAGGAGGGC TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC 
CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG AGCAGCTCAG AGTCCAGCGC AGGGTCCACC 
CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG AGCTGCGGGG TCCCCCTCTC AGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601404.1
CDS	102..3647
Translation	MRFWLRNEEMALEEMVQRLNAVSARTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_005601347.2
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601347.2

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCGGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CCGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAATGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGATGACAC GGTCACTAAC TCTACCTCAG AAAGCTCTGC ACTTTCCAAA 
AAGCGCTTTA CCCTGCAGGG TTTTGCTAAC CTCAAAGGTC AGAAAGCTTC TCCCACCAGT 
CCTGACAAAA AAGCTAAACG GCACGAAGTA AAGAGCGACC CCACGCCTTT TGGTTTACGA 
GGTTGGAGCA AAACGTCTCA GCCCTCCGAG GCCCCTGAGG ACAACGACGG CTGGTCGAGT 
GCTGAGGAGC CCCTTAATTC CTCGGACGCA GAGGAAGAAG GCGGAGTGGG CCCCAGGAAG 
CTGGTCCCAG GGAGGTTCAC AGTTGTGGCG GATGACGAGA AGGGCAGTCC TGACATGCTG 
GTCATGAGGA GCGGGGACCT GGTGGAGGTG GTCCAGGAGG GCGAGGACTG CCTCTGGTAT 
GTCAGGAACC TGACCTCCAG CAAGGAGGGC TGGGTGCCGG CCGGCAGCCT GTCCGCGCGC 
CTCGGCAAGT CCAGCTCAGC TCAGTGCTTG AGCAGCTCAG AGTCCAGCGC AGGGTCCACC 
CTGCTGAGCA GCTCCTCCAG CTGCAGTGAG AGCTGCGGGG TCCCCCTCTC AGACCTGCAG 
GGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_005601348.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601405.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.

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CloneID	OHb08032
Clone ID Related Accession (Same CDS sequence)	XM_005601348.2
Accession Version	XM_005601348.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005601348.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTAA GCCTCATCTC CGTGCTGTCG CAGGATATCT CCGGGCTGTG GCTCCTGCTG 
AAGGCGAGCA CAGATGAAAT CATGCACCAG GACATCACCC CGCTCTGCGC CGCCGACATC 
CAGGACCAGC TGAGGAAGCG CTTTGCCTAT CTGTCCGGCG GGCGGGGGCA GGACGGCAGC 
CCCGTCATCA CCTTCCCGGA CTACCCGGCC TTCAGCGACG TCCCAGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGCTTACAAG ATGCTGGCAT TGGGTTCATC 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCGGTGA AGGCATCAGT CCTGCGAATA 
GCAGCGTCCT TCCCGGCAAA CCTCCAGCGC GTCCTTGTCC TGCGCCCAAC GGGATTTTTC 
CAGAGGACCC TCTCTGACCT CGCTTTCAGA TTCAACAGAG ATGACTTTAA GATGAAGGTA 
CCGGTCACAA TGCTGAGCTC AGTACCAGAA TTGCACGATT ACATTGACAA GTCCCAGCTG 
ACGGAGGACC TGGGCGGGAC CCTGGATTAC TGCCACACCA AGTGGTTGTG TCACCGCACA 
GCCATTGAGA GTTTTGCTGT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACAGAGCTGG CCGAGACGGA ACTGCCCAAC GACGTCCCAT CCACAAGCTC TGTGCTCATC 
GCACACACGG AGAAGAAGCA CAAAGCAAAG GAGAACATGA GGCTGGCGCT GGACGAGGGG 
CGGAGCATCC TGGAGAGCAT CAGGGAGCCT CTGGCCAGGA GCACGGAGCA GAGCCCGAAC 
CAGGACCAGC TGGACAATCA GACCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAAACC 
GAGGCCGCCT TTGATGAGTT TTGGGCAAAG CATCAGCAAA AACTCGAACA GTGTCTGCAG 
CTCCGGCATT TTGAGCAGGA TTTCCGAGAG GTCAAAGCGG CCTTGGACAC GTTGGCCCAG 
AAGATAGTGA CCTTCACCGA CGTGGGGAAC AGCCTGGCGC ACGTGGAGCA CCTCCTGAAG 
GATCTCACGA GCTTCGAGGA GAAGTCCAGT GCAGCCGTGG AGCGGGCCCG CGCCTTGTCC 
CTGGAGGGTG AGCAGCTCAT CGGCAACAAG CACTATGCGG TGGACTCCAT CCGCCCCAAG 
TGCCACGAGC TGCAGCACCT CTGCGACCAG TTTGCCGCCG AGGTCGGGCG GAGGAGGGGG 
CTGCTCGGCA AGTCGCTGGA GCTGCACAGC CTCCTGGAGG CGTCCATGAA GTGGTGCGAT 
GAAGGCATCT ACCTGCTGGC CTCCCAGCCT GTGGACAAGT GCCAGTCCCA GGACGGCGCA 
GAGGCCGCCC TCCAGGAGAT CGAGAAGTTT CTGGAGACCG GCGCAGAAAA TAAGATCCAG 
GAGCTCAATA AAATTTACCA GGACTACGAA CTCATCCTCA CCCAAGACCT GAGGGAACAC 
GTGCAGAAGG TCTTCCAGAA GCAAGAGAGC ATGGAAGAGA TGTTCCACCG GAGGCAGGCG 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCCGGCCC 
GAGGCGCTCG CCAAGTCGCC CTGCGCCTCC CCAGGTATCC GGAGAGGTTC TGAGAACAGC 
AGTTGCGAGG GCAGCGCGCA GAGAGGGCCC TACAGGAGGG CCAAGAGTGA AATGAGTGAG 
GGCCGGCAGG GCCGAGGCAG CTCCACGGGG GACGAGGAGG AGAGCCTGGC CATCCTGCGG 
AGGCACGTCA TGAACGAGCT CCTTGACACG GAACGGGTCT ACGTAGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCTGC CGAGATGGAC AACCCGTTAA TGACACATCT CATCTCAACA 
GGCCTTCAAA ACAAGAAGGA TGTTCTATTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGAATAT TCCTGAGGGA GCTAGAAAAC TACATAGACT GCCCAGAGCT GGTCGGAAGG 
TGTTTTCTAG AAAGGCTGGA GGAGTTCCAG ATGTACGAGA AGTACTGTCA GAACAAGCCG 
CGTTCCGAGA GCGTGTGGCG GCAGTGCGCC GACTGCCCCT TCTTCCAGGA ATGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA AGAGCTGTGA GGGGGCCGAG 
GACTTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CCATCACGGG CTATGACGGG AATCTGAGCG ACCTGGGGAA GCTGCTCATG 
CAGGGCTCCT TCAGCGTCTG GACGGACCAC AAGAAGGGCC ACAGCAAGGT GAAGGACCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCGGT GCTCTTCTGC 
AAGAAGAGAG AGGAGAATGG GGAGGGCTAT GAGAAAGCGC CTTCCTACAG CTACAAGCAG 
TCCCTGAACA TGGCGGCGGT TGGCATAACA GAGAACGTGA AGGGCGACGT GAGGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTT TACATCATAC AGGCACCAAC TCCCGAAATC 
AAAGCGGCGT GGGTGAGCGA AATCCGGAAG CTGCTGACCA GCCAGCTGCA GGCCTTCAGA 
GAAGCCAGCC AGCACCGCGT ACTGGAGCAG TCCCAGAGCC TGCCTCTGCC TGCAACGGCC 
AGCGCAAGTC CCTCGAAAGG GAACACAAGA AACGTCAAAA AGCTGGAAGA AAGGAAGACC 
GACCCCCTCA GCCTGGAAGG ATACGTGAGC TCGACATCGC TGCCAAAGGC CCCCGAGAAG 
GGCAGAGATG ACACGGTCAC TAACTCTACC TCAGAAAGCT CTGCACTTTC CAAAAAGCGC 
TTTACCCTGC AGGGTTTTGC TAACCTCAAA GGTCAGAAAG CTTCTCCCAC CAGTCCTGAC 
AAAAAAGCTA AACGGCACGA AGTAAAGAGC GACCCCACGC CTTTTGGTTT ACGAGGTTGG 
AGCAAAACGT CTCAGCCCTC CGAGGCCCCT GAGGACAACG ACGGCTGGTC GAGTGCTGAG 
GAGCCCCTTA ATTCCTCGGA CGCAGAGGAA GAAGGCGGAG TGGGCCCCAG GAAGCTGGTC 
CCAGGGAGGT TCACAGTTGT GGCGGATGAC GAGAAGGGCA GTCCTGACAT GCTGGTCATG 
AGGAGCGGGG ACCTGGTGGA GGTGGTCCAG GAGGGCGAGG ACTGCCTCTG GTATGTCAGG 
AACCTGACCT CCAGCAAGGA GGGCTGGGTG CCGGCCGGCA GCCTGTCCGC GCGCCTCGGC 
AAGTCCAGCT CAGCTCAGTG CTTGAGCAGC TCAGAGTCCA GCGCAGGGTC CACCCTGCTG 
AGCAGCTCCT CCAGCTGCAG TGAGAGCTGC GGGGTCCCCC TCTCAGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601405.1
CDS	184..3723
Translation	MTLSLISVLSQDISGLWLLLKASTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRDDTVTNSTSESSALSKKRFTLQGFANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_005601348.2
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601348.2

ORF Insert Sequence:

ATGACCTTAA GCCTCATCTC CGTGCTGTCG CAGGATATCT CCGGGCTGTG GCTCCTGCTG 
AAGGCGAGCA CAGATGAAAT CATGCACCAG GACATCACCC CGCTCTGCGC CGCCGACATC 
CAGGACCAGC TGAGGAAGCG CTTTGCCTAT CTGTCCGGCG GGCGGGGGCA GGACGGCAGC 
CCCGTCATCA CCTTCCCGGA CTACCCGGCC TTCAGCGACG TCCCAGACAA GGAGTTCCAG 
AATGTCATGA CCTATCTCAC CAGCATCCCC AGCTTACAAG ATGCTGGCAT TGGGTTCATC 
CTGGTCATAG ACCGAAGGCA GGACAAATGG ACCTCGGTGA AGGCATCAGT CCTGCGAATA 
GCAGCGTCCT TCCCGGCAAA CCTCCAGCGC GTCCTTGTCC TGCGCCCAAC GGGATTTTTC 
CAGAGGACCC TCTCTGACCT CGCTTTCAGA TTCAACAGAG ATGACTTTAA GATGAAGGTA 
CCGGTCACAA TGCTGAGCTC AGTACCAGAA TTGCACGATT ACATTGACAA GTCCCAGCTG 
ACGGAGGACC TGGGCGGGAC CCTGGATTAC TGCCACACCA AGTGGTTGTG TCACCGCACA 
GCCATTGAGA GTTTTGCTGT CATGGTGAAG CAGACGGCTC AGATGCTGCA GTCCTTCGGG 
ACAGAGCTGG CCGAGACGGA ACTGCCCAAC GACGTCCCAT CCACAAGCTC TGTGCTCATC 
GCACACACGG AGAAGAAGCA CAAAGCAAAG GAGAACATGA GGCTGGCGCT GGACGAGGGG 
CGGAGCATCC TGGAGAGCAT CAGGGAGCCT CTGGCCAGGA GCACGGAGCA GAGCCCGAAC 
CAGGACCAGC TGGACAATCA GACCACCGTG CAGAGGCTCC TGGCCCAGCT GAATGAAACC 
GAGGCCGCCT TTGATGAGTT TTGGGCAAAG CATCAGCAAA AACTCGAACA GTGTCTGCAG 
CTCCGGCATT TTGAGCAGGA TTTCCGAGAG GTCAAAGCGG CCTTGGACAC GTTGGCCCAG 
AAGATAGTGA CCTTCACCGA CGTGGGGAAC AGCCTGGCGC ACGTGGAGCA CCTCCTGAAG 
GATCTCACGA GCTTCGAGGA GAAGTCCAGT GCAGCCGTGG AGCGGGCCCG CGCCTTGTCC 
CTGGAGGGTG AGCAGCTCAT CGGCAACAAG CACTATGCGG TGGACTCCAT CCGCCCCAAG 
TGCCACGAGC TGCAGCACCT CTGCGACCAG TTTGCCGCCG AGGTCGGGCG GAGGAGGGGG 
CTGCTCGGCA AGTCGCTGGA GCTGCACAGC CTCCTGGAGG CGTCCATGAA GTGGTGCGAT 
GAAGGCATCT ACCTGCTGGC CTCCCAGCCT GTGGACAAGT GCCAGTCCCA GGACGGCGCA 
GAGGCCGCCC TCCAGGAGAT CGAGAAGTTT CTGGAGACCG GCGCAGAAAA TAAGATCCAG 
GAGCTCAATA AAATTTACCA GGACTACGAA CTCATCCTCA CCCAAGACCT GAGGGAACAC 
GTGCAGAAGG TCTTCCAGAA GCAAGAGAGC ATGGAAGAGA TGTTCCACCG GAGGCAGGCG 
AGCCTGAAGA AGCTGGCGGC CAAGCAGACG CGGCCCGTGC AGCCGGTGGC CCCCCGGCCC 
GAGGCGCTCG CCAAGTCGCC CTGCGCCTCC CCAGGTATCC GGAGAGGTTC TGAGAACAGC 
AGTTGCGAGG GCAGCGCGCA GAGAGGGCCC TACAGGAGGG CCAAGAGTGA AATGAGTGAG 
GGCCGGCAGG GCCGAGGCAG CTCCACGGGG GACGAGGAGG AGAGCCTGGC CATCCTGCGG 
AGGCACGTCA TGAACGAGCT CCTTGACACG GAACGGGTCT ACGTAGAGGA GCTGCTGTGC 
GTCCTGGAGG GCTACGCTGC CGAGATGGAC AACCCGTTAA TGACACATCT CATCTCAACA 
GGCCTTCAAA ACAAGAAGGA TGTTCTATTT GGAAACATGG AAGAAATTTA CCACTTTCAT 
AACAGAATAT TCCTGAGGGA GCTAGAAAAC TACATAGACT GCCCAGAGCT GGTCGGAAGG 
TGTTTTCTAG AAAGGCTGGA GGAGTTCCAG ATGTACGAGA AGTACTGTCA GAACAAGCCG 
CGTTCCGAGA GCGTGTGGCG GCAGTGCGCC GACTGCCCCT TCTTCCAGGA ATGCCAGAAG 
AAGCTGGACC ACAAGCTGAG CCTGGACTCC TACCTGCTGA AGCCAGTCCA GAGGATCACC 
AAGTACCAGC TGCTGCTCAA GGAAATGCTG AAATACAGCA AGAGCTGTGA GGGGGCCGAG 
GACTTGCAGG AGGCGCTGAG CTCCATCCTG GGCATCCTCA AGGCCGTGAA CGACTCCATG 
CACCTCATCG CCATCACGGG CTATGACGGG AATCTGAGCG ACCTGGGGAA GCTGCTCATG 
CAGGGCTCCT TCAGCGTCTG GACGGACCAC AAGAAGGGCC ACAGCAAGGT GAAGGACCTG 
GCCAGGTTCA AGCCCATGCA GCGGCACCTG TTCCTGCACG AGAAGGCGGT GCTCTTCTGC 
AAGAAGAGAG AGGAGAATGG GGAGGGCTAT GAGAAAGCGC CTTCCTACAG CTACAAGCAG 
TCCCTGAACA TGGCGGCGGT TGGCATAACA GAGAACGTGA AGGGCGACGT GAGGAAGTTT 
GAGATCTGGT ACAACGCCCG GGAGGAGGTT TACATCATAC AGGCACCAAC TCCCGAAATC 
AAAGCGGCGT GGGTGAGCGA AATCCGGAAG CTGCTGACCA GCCAGCTGCA GGCCTTCAGA 
GAAGCCAGCC AGCACCGCGT ACTGGAGCAG TCCCAGAGCC TGCCTCTGCC TGCAACGGCC 
AGCGCAAGTC CCTCGAAAGG GAACACAAGA AACGTCAAAA AGCTGGAAGA AAGGAAGACC 
GACCCCCTCA GCCTGGAAGG ATACGTGAGC TCGACATCGC TGCCAAAGGC CCCCGAGAAG 
GGCAGAGATG ACACGGTCAC TAACTCTACC TCAGAAAGCT CTGCACTTTC CAAAAAGCGC 
TTTACCCTGC AGGGTTTTGC TAACCTCAAA GGTCAGAAAG CTTCTCCCAC CAGTCCTGAC 
AAAAAAGCTA AACGGCACGA AGTAAAGAGC GACCCCACGC CTTTTGGTTT ACGAGGTTGG 
AGCAAAACGT CTCAGCCCTC CGAGGCCCCT GAGGACAACG ACGGCTGGTC GAGTGCTGAG 
GAGCCCCTTA ATTCCTCGGA CGCAGAGGAA GAAGGCGGAG TGGGCCCCAG GAAGCTGGTC 
CCAGGGAGGT TCACAGTTGT GGCGGATGAC GAGAAGGGCA GTCCTGACAT GCTGGTCATG 
AGGAGCGGGG ACCTGGTGGA GGTGGTCCAG GAGGGCGAGG ACTGCCTCTG GTATGTCAGG 
AACCTGACCT CCAGCAAGGA GGGCTGGGTG CCGGCCGGCA GCCTGTCCGC GCGCCTCGGC 
AAGTCCAGCT CAGCTCAGTG CTTGAGCAGC TCAGAGTCCA GCGCAGGGTC CACCCTGCTG 
AGCAGCTCCT CCAGCTGCAG TGAGAGCTGC GGGGTCCCCC TCTCAGACCT GCAGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCF2L ( XM_005601350.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005601407.1 Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.

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CloneID	OHb08034
Clone ID Related Accession (Same CDS sequence)	XM_005601350.2
Accession Version	XM_005601350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005601350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCGGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CCGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAATGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC AGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACATGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005601407.1
CDS	101..3478
Translation	MRFWLRNEEMALEEMVQRLNAVSARTDEIMHQDITPLCAADIQDQLRKRFAYLSGGRGQDGSPVITFPDYPAFSDVPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRQDKWTSVKASVLRIAASFPANLQRVLVLRPTGFFQRTLSDLAFRFNRDDFKMKVPVTMLSSVPELHDYIDKSQLTEDLGGTLDYCHTKWLCHRTAIESFAVMVKQTAQMLQSFGTELAETELPNDVPSTSSVLIAHTEKKHKAKENMRLALDEGRSILESIREPLARSTEQSPNQDQLDNQTTVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQDFREVKAALDTLAQKIVTFTDVGNSLAHVEHLLKDLTSFEEKSSAAVERARALSLEGEQLIGNKHYAVDSIRPKCHELQHLCDQFAAEVGRRRGLLGKSLELHSLLEASMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNKIYQDYELILTQDLREHVQKVFQKQESMEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALAKSPCASPGIRRGSENSSCEGSAQRGPYRRAKSEMSEGRQGRGSSTGDEEESLAILRRHVMNELLDTERVYVEELLCVLEGYAAEMDNPLMTHLISTGLQNKKDVLFGNMEEIYHFHNRIFLRELENYIDCPELVGRCFLERLEEFQMYEKYCQNKPRSESVWRQCADCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKSCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLSDLGKLLMQGSFSVWTDHKKGHSKVKDLARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDVRKFEIWYNAREEVYIIQAPTPEIKAAWVSEIRKLLTSQLQAFREASQHRVLEQSQSLPLPATASASPSKGNTRNVKKLEERKTDPLSLEGYVSSTSLPKAPEKGRGWSKTSQPSEAPEDNDGWSSAEEPLNSSDAEEEGGVGPRKLVPGRFTVVADDEKGSPDMLVMRSGDLVEVVQEGEDCLWYVRNLTSSKEGWVPAGSLSARLGKSSSAQCLSSSESSAGSTLLSSSSSCSESCGVPLSDLQG

Target ORF information:

RefSeq Version	XM_005601350.2
Organism	Equus caballus(horse)
Definition	Equus caballus MCF.2 cell line derived transforming sequence like (MCF2L), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005601350.2

ORF Insert Sequence:

ATGAGGTTTT GGCTGAGAAA TGAAGAGATG GCCCTGGAAG AAATGGTGCA GAGATTAAAT 
GCGGTTTCCG CGCGCACGGA TGAAATCATG CACCAGGACA TCACCCCGCT CTGCGCCGCC 
GACATCCAGG ACCAGCTGAG GAAGCGCTTT GCCTATCTGT CCGGCGGGCG GGGGCAGGAC 
GGCAGCCCCG TCATCACCTT CCCGGACTAC CCGGCCTTCA GCGACGTCCC AGACAAGGAG 
TTCCAGAATG TCATGACCTA TCTCACCAGC ATCCCCAGCT TACAAGATGC TGGCATTGGG 
TTCATCCTGG TCATAGACCG AAGGCAGGAC AAATGGACCT CGGTGAAGGC ATCAGTCCTG 
CGAATAGCAG CGTCCTTCCC GGCAAACCTC CAGCGCGTCC TTGTCCTGCG CCCAACGGGA 
TTTTTCCAGA GGACCCTCTC TGACCTCGCT TTCAGATTCA ACAGAGATGA CTTTAAGATG 
AAGGTACCGG TCACAATGCT GAGCTCAGTA CCAGAATTGC ACGATTACAT TGACAAGTCC 
CAGCTGACGG AGGACCTGGG CGGGACCCTG GATTACTGCC ACACCAAGTG GTTGTGTCAC 
CGCACAGCCA TTGAGAGTTT TGCTGTCATG GTGAAGCAGA CGGCTCAGAT GCTGCAGTCC 
TTCGGGACAG AGCTGGCCGA GACGGAACTG CCCAACGACG TCCCATCCAC AAGCTCTGTG 
CTCATCGCAC ACACGGAGAA GAAGCACAAA GCAAAGGAGA ACATGAGGCT GGCGCTGGAC 
GAGGGGCGGA GCATCCTGGA GAGCATCAGG GAGCCTCTGG CCAGGAGCAC GGAGCAGAGC 
CCGAACCAGG ACCAGCTGGA CAATCAGACC ACCGTGCAGA GGCTCCTGGC CCAGCTGAAT 
GAAACCGAGG CCGCCTTTGA TGAGTTTTGG GCAAAGCATC AGCAAAAACT CGAACAGTGT 
CTGCAGCTCC GGCATTTTGA GCAGGATTTC CGAGAGGTCA AAGCGGCCTT GGACACGTTG 
GCCCAGAAGA TAGTGACCTT CACCGACGTG GGGAACAGCC TGGCGCACGT GGAGCACCTC 
CTGAAGGATC TCACGAGCTT CGAGGAGAAG TCCAGTGCAG CCGTGGAGCG GGCCCGCGCC 
TTGTCCCTGG AGGGTGAGCA GCTCATCGGC AACAAGCACT ATGCGGTGGA CTCCATCCGC 
CCCAAGTGCC ACGAGCTGCA GCACCTCTGC GACCAGTTTG CCGCCGAGGT CGGGCGGAGG 
AGGGGGCTGC TCGGCAAGTC GCTGGAGCTG CACAGCCTCC TGGAGGCGTC CATGAAGTGG 
TGCGATGAAG GCATCTACCT GCTGGCCTCC CAGCCTGTGG ACAAGTGCCA GTCCCAGGAC 
GGCGCAGAGG CCGCCCTCCA GGAGATCGAG AAGTTTCTGG AGACCGGCGC AGAAAATAAG 
ATCCAGGAGC TCAATAAAAT TTACCAGGAC TACGAACTCA TCCTCACCCA AGACCTGAGG 
GAACACGTGC AGAAGGTCTT CCAGAAGCAA GAGAGCATGG AAGAGATGTT CCACCGGAGG 
CAGGCGAGCC TGAAGAAGCT GGCGGCCAAG CAGACGCGGC CCGTGCAGCC GGTGGCCCCC 
CGGCCCGAGG CGCTCGCCAA GTCGCCCTGC GCCTCCCCAG GTATCCGGAG AGGTTCTGAG 
AACAGCAGTT GCGAGGGCAG CGCGCAGAGA GGGCCCTACA GGAGGGCCAA GAGTGAAATG 
AGTGAGGGCC GGCAGGGCCG AGGCAGCTCC ACGGGGGACG AGGAGGAGAG CCTGGCCATC 
CTGCGGAGGC ACGTCATGAA CGAGCTCCTT GACACGGAAC GGGTCTACGT AGAGGAGCTG 
CTGTGCGTCC TGGAGGGCTA CGCTGCCGAG ATGGACAACC CGTTAATGAC ACATCTCATC 
TCAACAGGCC TTCAAAACAA GAAGGATGTT CTATTTGGAA ACATGGAAGA AATTTACCAC 
TTTCATAACA GAATATTCCT GAGGGAGCTA GAAAACTACA TAGACTGCCC AGAGCTGGTC 
GGAAGGTGTT TTCTAGAAAG GCTGGAGGAG TTCCAGATGT ACGAGAAGTA CTGTCAGAAC 
AAGCCGCGTT CCGAGAGCGT GTGGCGGCAG TGCGCCGACT GCCCCTTCTT CCAGGAATGC 
CAGAAGAAGC TGGACCACAA GCTGAGCCTG GACTCCTACC TGCTGAAGCC AGTCCAGAGG 
ATCACCAAGT ACCAGCTGCT GCTCAAGGAA ATGCTGAAAT ACAGCAAGAG CTGTGAGGGG 
GCCGAGGACT TGCAGGAGGC GCTGAGCTCC ATCCTGGGCA TCCTCAAGGC CGTGAACGAC 
TCCATGCACC TCATCGCCAT CACGGGCTAT GACGGGAATC TGAGCGACCT GGGGAAGCTG 
CTCATGCAGG GCTCCTTCAG CGTCTGGACG GACCACAAGA AGGGCCACAG CAAGGTGAAG 
GACCTGGCCA GGTTCAAGCC CATGCAGCGG CACCTGTTCC TGCACGAGAA GGCGGTGCTC 
TTCTGCAAGA AGAGAGAGGA GAATGGGGAG GGCTATGAGA AAGCGCCTTC CTACAGCTAC 
AAGCAGTCCC TGAACATGGC GGCGGTTGGC ATAACAGAGA ACGTGAAGGG CGACGTGAGG 
AAGTTTGAGA TCTGGTACAA CGCCCGGGAG GAGGTTTACA TCATACAGGC ACCAACTCCC 
GAAATCAAAG CGGCGTGGGT GAGCGAAATC CGGAAGCTGC TGACCAGCCA GCTGCAGGCC 
TTCAGAGAAG CCAGCCAGCA CCGCGTACTG GAGCAGTCCC AGAGCCTGCC TCTGCCTGCA 
ACGGCCAGCG CAAGTCCCTC GAAAGGGAAC ACAAGAAACG TCAAAAAGCT GGAAGAAAGG 
AAGACCGACC CCCTCAGCCT GGAAGGATAC GTGAGCTCGA CATCGCTGCC AAAGGCCCCC 
GAGAAGGGCA GAGGTTGGAG CAAAACGTCT CAGCCCTCCG AGGCCCCTGA GGACAACGAC 
GGCTGGTCGA GTGCTGAGGA GCCCCTTAAT TCCTCGGACG CAGAGGAAGA AGGCGGAGTG 
GGCCCCAGGA AGCTGGTCCC AGGGAGGTTC ACAGTTGTGG CGGATGACGA GAAGGGCAGT 
CCTGACATGC TGGTCATGAG GAGCGGGGAC CTGGTGGAGG TGGTCCAGGA GGGCGAGGAC 
TGCCTCTGGT ATGTCAGGAA CCTGACCTCC AGCAAGGAGG GCTGGGTGCC GGCCGGCAGC 
CTGTCCGCGC GCCTCGGCAA GTCCAGCTCA GCTCAGTGCT TGAGCAGCTC AGAGTCCAGC 
GCAGGGTCCA CCCTGCTGAG CAGCTCCTCC AGCTGCAGTG AGAGCTGCGG GGTCCCCCTC 
TCAGACCTGC AGGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MCF2L
Entrez Gene ID	100067048
Full Name	MCF.2 cell line derived transforming sequence like
General protein information	Preferred Names MCF.2 cell line derived transforming sequence like Names guanine nucleotide exchange factor DBS
Gene Type	protein-coding
Organism	Equus caballus(horse)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MCF2L cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

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