DENND4B cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND4B
Entrez Gene ID	100062197
Full Name	DENN domain containing 4B
General protein information	Preferred Names DENN domain containing 4B Names DENN domain-containing protein 4B
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005610096.2	XP_005610153.1	DENN domain-containing protein 4B isoform X2
XM_005610096.3	XP_005610153.1	DENN domain-containing protein 4B isoform X3
XM_023641110.1	XP_023496878.1	DENN domain-containing protein 4B isoform X2
XM_023641111.1	XP_023496879.1	DENN domain-containing protein 4B isoform X2
XM_001493858.5	XP_001493908.3	DENN domain-containing protein 4B isoform X1
XM_014739909.1	XP_014595395.1	DENN domain-containing protein 4B isoform X3
XM_001493858.6	XP_001493908.3	DENN domain-containing protein 4B isoform X1

Pathways from BioSystems
Gene Ontology

The following DENND4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb20932	XM_005610096.3 Latest version!	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHb30755	XM_014739909.1 Latest version!	Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb65322	XM_023641111.1 Latest version!	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHb65322	XM_023641110.1 Latest version!	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHb20931	XM_001493858.6 Latest version!	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OHb20932	XM_005610096.2	Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHb20931	XM_001493858.5	Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND4B ( XM_005610096.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005610153.1 Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb20932
Clone ID Related Accession (Same CDS sequence)	XM_005610096.2 , XM_005610096.3
Accession Version	XM_005610096.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867419.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005610096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGTGCGGCT GCTGTGGGAT GTCCTGACCC CCGACCCCAA TAGCTGCCCA 
CCTCTGTATG TGCTCTGGAG AGTCCACAGC CAGATCCCCC AGCGGGTAGT ATGGCCAGGC 
CCAGTACCTG CCTCCCTTAG CCTGGCGTTG CTGGAGTCGG TGCTGCGCCA TGTTGGTCTC 
AATGAGGTGC ACAAGGCTGT AGGGCTCCTG CTGGAAACTC TAGGGCCTCC TCCCACAGGC 
CTGCACCTAC AGAGGGGCAT CTACCGTGAG ATCTTATTCC TGACAATGGC TGCTATGGGC 
AAGGACCACA TGGACATAGT GGCCTTCGAC AAGAAGTACA AGTCTGCCTT TAACAAGCTG 
GCCAGCAGCA TGGGCAAGGA GGAGCTGAGG CAGCGGCGGG CACAGATGCC CACTCCAAAG 
GCCATTGATT GCCGAAAATG TTTTGGAGCA CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005610153.1
CDS	745..5229
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAMYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATVECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLLPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLRTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVPEGSEPTPQYCYDGFPELQAELFESPQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVQVMLEMLRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERRKQRQQQQQQQQQQKQQAAAQQKTGSSQTEPSLERPSPTRPLHRQTTWAGRSLRDPASPSGRLVKSGSLGSAQGAQPTVEAGVAHMIEALGVLKPRGSPVPWHDGSLSDLSLTGEEPAPGGSPGDSGSALSTQSSEALEGLSGRVPKASGHQDEASTPRRGLGARLQQLLTPSRRSPTSRIPPPELPPDLPPPARRSPMDSLLRPRERPGSTASESSASLGSEWDLSESSVSSLSLRHSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDFRPSAPSPKPAPADAGGNKDAPVPGGPGPVLSNRRLCLALDEPQLCNGHMGGASRRVEGGAWAYLSPLVLRKELESLIENEGSEVLALPELPATHPIIFWNLLWYFQRLQLPSILPCLVLASCDGPPPPQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAAMGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_005610096.2
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005610096.2

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGTGCGGCT GCTGTGGGAT GTCCTGACCC CCGACCCCAA TAGCTGCCCA 
CCTCTGTATG TGCTCTGGAG AGTCCACAGC CAGATCCCCC AGCGGGTAGT ATGGCCAGGC 
CCAGTACCTG CCTCCCTTAG CCTGGCGTTG CTGGAGTCGG TGCTGCGCCA TGTTGGTCTC 
AATGAGGTGC ACAAGGCTGT AGGGCTCCTG CTGGAAACTC TAGGGCCTCC TCCCACAGGC 
CTGCACCTAC AGAGGGGCAT CTACCGTGAG ATCTTATTCC TGACAATGGC TGCTATGGGC 
AAGGACCACA TGGACATAGT GGCCTTCGAC AAGAAGTACA AGTCTGCCTT TAACAAGCTG 
GCCAGCAGCA TGGGCAAGGA GGAGCTGAGG CAGCGGCGGG CACAGATGCC CACTCCAAAG 
GCCATTGATT GCCGAAAATG TTTTGGAGCA CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_005610096.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005610153.1 Equus caballus DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHb20932
Clone ID Related Accession (Same CDS sequence)	XM_005610096.2 , XM_005610096.3
Accession Version	XM_005610096.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005610096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGTGCGGCT GCTGTGGGAT GTCCTGACCC CCGACCCCAA TAGCTGCCCA 
CCTCTGTATG TGCTCTGGAG AGTCCACAGC CAGATCCCCC AGCGGGTAGT ATGGCCAGGC 
CCAGTACCTG CCTCCCTTAG CCTGGCGTTG CTGGAGTCGG TGCTGCGCCA TGTTGGTCTC 
AATGAGGTGC ACAAGGCTGT AGGGCTCCTG CTGGAAACTC TAGGGCCTCC TCCCACAGGC 
CTGCACCTAC AGAGGGGCAT CTACCGTGAG ATCTTATTCC TGACAATGGC TGCTATGGGC 
AAGGACCACA TGGACATAGT GGCCTTCGAC AAGAAGTACA AGTCTGCCTT TAACAAGCTG 
GCCAGCAGCA TGGGCAAGGA GGAGCTGAGG CAGCGGCGGG CACAGATGCC CACTCCAAAG 
GCCATTGATT GCCGAAAATG TTTTGGAGCA CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005610153.1
CDS	1039..5523
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAMYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATVECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLLPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLRTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVPEGSEPTPQYCYDGFPELQAELFESPQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVQVMLEMLRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERRKQRQQQQQQQQQQKQQAAAQQKTGSSQTEPSLERPSPTRPLHRQTTWAGRSLRDPASPSGRLVKSGSLGSAQGAQPTVEAGVAHMIEALGVLKPRGSPVPWHDGSLSDLSLTGEEPAPGGSPGDSGSALSTQSSEALEGLSGRVPKASGHQDEASTPRRGLGARLQQLLTPSRRSPTSRIPPPELPPDLPPPARRSPMDSLLRPRERPGSTASESSASLGSEWDLSESSVSSLSLRHSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDFRPSAPSPKPAPADAGGNKDAPVPGGPGPVLSNRRLCLALDEPQLCNGHMGGASRRVEGGAWAYLSPLVLRKELESLIENEGSEVLALPELPATHPIIFWNLLWYFQRLQLPSILPCLVLASCDGPPPPQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAAMGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_005610096.3
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005610096.3

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGTGCGGCT GCTGTGGGAT GTCCTGACCC CCGACCCCAA TAGCTGCCCA 
CCTCTGTATG TGCTCTGGAG AGTCCACAGC CAGATCCCCC AGCGGGTAGT ATGGCCAGGC 
CCAGTACCTG CCTCCCTTAG CCTGGCGTTG CTGGAGTCGG TGCTGCGCCA TGTTGGTCTC 
AATGAGGTGC ACAAGGCTGT AGGGCTCCTG CTGGAAACTC TAGGGCCTCC TCCCACAGGC 
CTGCACCTAC AGAGGGGCAT CTACCGTGAG ATCTTATTCC TGACAATGGC TGCTATGGGC 
AAGGACCACA TGGACATAGT GGCCTTCGAC AAGAAGTACA AGTCTGCCTT TAACAAGCTG 
GCCAGCAGCA TGGGCAAGGA GGAGCTGAGG CAGCGGCGGG CACAGATGCC CACTCCAAAG 
GCCATTGATT GCCGAAAATG TTTTGGAGCA CCTCTGGAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_014739909.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014595395.1 Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X3, mRNA.

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CloneID	OHb30755
Clone ID Related Accession (Same CDS sequence)	XM_014739909.1
Accession Version	XM_014739909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	DENN domain-containing protein 4B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867419.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGG CATTACTGAC CTCTGCCTGG TGCTGCCCAG CAAGGGCGAG GGCACCCCTC 
ACACGTACTG CCGATTGCCC CGCAACCTCA ACCCTGGCAT GTGGGGCCCA GCGATGTACC 
TGTGCTACAA GGTGGGCCTG GCCAAGGCCA ACACTCTGGT GTATGAGGCA GAACTGCTGG 
GCCGCTACCC AGAGGAGGAC AATGAGGCGT TCCCGCTGCC CGAGTCCGTG CCGGTCTTCT 
GCCTGCCCAT GGGGGCCACT GTCGAGTGCT GGCCCGCCCA GACCAAGTAC CCCGTACCCG 
TCTTCTCCAC CTTTGTGCTC ACGGGCGCAG CTGGTGATAA GGCACATCTC CCACTTCATT 
CACAACGTCC CCTTCCCTTC CCCACAGAGA CCCCGCATCC TGGTGCAGAT GTCCCCGTAT 
GACAACCTGC TCCTCTGCCA GCCTGTGTCC TCACCCTTGC CCCTCAGTGG TGCCAGCTTC 
CTGCAGCTGC TGCAGAGCCT GGGCCCTGAG CTGGCTATCA CGCTGCTGCT GGCTGTGCTC 
ACAGAGCACA AACTGCTAGT CCACTCGCTG CGGCCGGACC TGCTCACCAG TGTCTGCGAG 
GCCCTCGTCT CTATGATCTT CCCGCTGCAC TGGCAGTGCC CCTACATTCC ACTGTGCCCA 
CTGGTGCTGG CAGATGTGCT GAGCGCCCCC GTGCCCTTCA TTGTGGGTAT CCACTCCAGC 
TACTTCGATC TGCATGACCC TCCTGCTGAT GTCATCTGTG TTGATCTTGA TACCAACACA 
CTCTTCCAGA CTGAGGAAAA GAAGCCCCTC TCCCCTCGGA CTCTGCCCCG CAGACCCTAC 
AAAGTTCTGT TGGCTACACT GACAAACCTA TATCAGCAGC TGGATCAGAC GTATACTGGA 
CCCGAGGAGG AGGCATCCCT GGAATTCCTG CTGACAGACT ACGAGGCAGT GTGCGGCCGC 
CGGGCCCGGC TGGAGCGTGA GGTCCAGGGA GCCTTCCTCC GCTTCATGGC CTGTCTGCTC 
AAGGGCTACC GGGACTTCCT GCTCCCGCTC ACCCAGGCCC CCTCGGAGGG GGCTCGTGAT 
GTTGACAACC TGTTCTTCCT GCAGGGCTTC CTCAAGTCCC GGGAACGCTC CAGCCATAAG 
CTGTACTCTC AGCTGCTACG CACACAGATG TTCTCGCAGT TCATCGAGGA ATGCTCTTTT 
GGCTCTGCTC GGCATGCTGC CCTTGAATTC TTTGACTCTT GTGTTGACAA GGTCCACCCA 
GAGCAGGAGA AGCCTGAACC GACCCCCCTA GTGGAGCTGG AGGAGCTGTC GGGAAGTGAG 
CTCACTGTGT TTATCACACC TCCTGAGGAG CCTCCAGTGC CAGAGGGCAG TGAACCCACT 
CCCCAGTACT GCTATGATGG GTTCCCAGAG CTACAGGCTG AGCTGTTTGA GTCTCCTCAA 
GAGCAGCCTG GGGCTCTGCC TGTGCCGGGC CCCTCCCGTA GTGCCCCCAG CAGTCCTGCC 
CCACGCCGTA CCAAACAGGA GATGAAGGTC GCACAACGGA TGGCGCAGAA GTCAGCAGCT 
GTGCCTGAGC TGTGGGCCCG GTGCCTTCTG GGGCACTGCT ACGGGCTGTG GTTCCTGTGT 
CTGCCTGCTT ACGTGCGCTC GGCGCCCTCC CGGGTGCGGG CACTGCACAC GGCCTACCAA 
GTGCTGCGCC AGATGGAGAG CCGCAAGGTG GTGCTGCCCG ATGAGGTGTG TTACCGGGTG 
CTGATGCAGC TCTGCTCACA CTATGGGCAG CCAGTGCTGT CTGTTCAGGT CATGCTAGAG 
ATGCTGCGAG CAGGCATTGT GCCCAACACC ATCACCTACG GCTACTACAA CAAGGCTGTG 
CTGGAAAGCA AGTGGCCGTC TGGTACACCG GGTGGGCGCC TGCGCTGGGC CAAGCTCCGG 
AACGTCGTCC TGGGGGCTGC TCAGTTCCGC CAGCCCTTGA GAGAACGGCG GAAGCAGCGG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG AAGCAGCAGG CAGCAGCACA ACAAAAGACA 
GGCAGCTCCC AGACAGAGCC CTCTCTGGAG CGCCCCTCCC CAACCCGCCC CCTTCATCGC 
CAGACTACTT GGGCTGGGCG AAGCCTGCGG GACCCAGCCT CACCCTCAGG GCGCCTGGTG 
AAGAGTGGCA GCCTGGGCAG TGCCCAAGGG GCACAGCCCA CTGTGGAGGC TGGCGTAGCC 
CACATGATAG AGGCCTTGGG GGTATTGAAA CCCCGAGGAT CACCTGTGCC CTGGCACGAT 
GGAAGCCTTT CAGACTTGAG CCTGACCGGG GAGGAGCCAG CACCTGGAGG AAGCCCAGGG 
GACTCTGGCT CAGCCCTGAG TACTCAGTCC AGTGAAGCCC TGGAAGGGCT AAGTGGCCGG 
GTACCCAAGG CTAGTGGGCA TCAGGATGAG GCCAGCACCC CCCGACGAGG GCTGGGTGCC 
CGCCTCCAAC AGCTGCTCAC TCCTTCCCGC CGCTCCCCGA CCTCTCGCAT TCCCCCACCT 
GAGCTGCCCC CTGACCTGCC TCCCCCAGCC CGCCGCAGCC CCATGGACAG CCTTCTGCGC 
CCCCGGGAGC GCCCTGGATC CACTGCCTCT GAGAGCTCAG CCTCTCTGGG CAGTGAGTGG 
GACCTCTCAG AATCTTCCGT CAGCAGCCTG AGCCTTCGCC ATTCCTCAGA GCGCCTCAGT 
GACACCCCTG GATCCTTCCA GCCACCTTCC CTGGAAATTC TGCTGTCTAG TTGCTCCTTG 
TGCCGCGCCT GTGATTCGCT CGTGTACGAT GAGGAGATCA TGGCTGGCTG GGCACCTGAT 
GACTCCAACC TCAACACAAC CTGCCCCTTC TGCGCATGCC CCTTTGTGCC CCTGCTCAGT 
GTCCAGACCC TTGATTTCCG ACCCAGTGCC CCCAGCCCCA AGCCTGCCCC TGCGGATGCC 
GGAGGCAACA AAGACGCACC TGTCCCTGGG GGCCCAGGAC CTGTGCTCAG TAACCGCAGG 
CTCTGCCTTG CCCTGGATGA GCCCCAGCTC TGCAATGGGC ACATGGGGGG TGCCTCCCGG 
CGGGTCGAGG GTGGGGCATG GGCATACCTG AGCCCCCTGG TGCTGCGTAA GGAGTTGGAG 
TCGCTGATAG AGAACGAGGG CAGCGAGGTG CTGGCATTGC CTGAGCTGCC TGCTACCCAC 
CCCATTATCT TCTGGAACCT TCTGTGGTAT TTCCAGCGAC TGCAACTGCC CAGTATTCTG 
CCATGCCTGG TGCTGGCCTC CTGTGATGGG CCCCCACCCC CCCAGGCCCC ATCTCCTTGG 
CTAACACCTG ATCCAGCCTC TGTGCAGGTG CGGCTGCTGT GGGATGTCCT GACCCCCGAC 
CCCAATAGCT GCCCACCTCT GTATGTGCTC TGGAGAGTCC ACAGCCAGAT CCCCCAGCGG 
GTAGTATGGC CAGGCCCAGT ACCTGCCTCC CTTAGCCTGG CGTTGCTGGA GTCGGTGCTG 
CGCCATGTTG GTCTCAATGA GGTGCACAAG GCTGTAGGGC TCCTGCTGGA AACTCTAGGG 
CCTCCTCCCA CAGGCCTGCA CCTACAGAGG GGCATCTACC GTGAGATCTT ATTCCTGACA 
ATGGCTGCTA TGGGCAAGGA CCACATGGAC ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAGC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CAAAGGCCAT TGATTGCCGA AAATGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014595395.1
CDS	82..3861
Translation	MPWALLTSAWCCPARARAPLTRTADCPATSTLACGAQRCTCATRWAWPRPTLWCMRQNCWAATQRRTMRRSRCPSPCRSSACPWGPLSSAGPPRPSTPYPSSPPLCSRAQLVIRHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLLPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLRTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVPEGSEPTPQYCYDGFPELQAELFESPQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVQVMLEMLRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERRKQRQQQQQQQQQQKQQAAAQQKTGSSQTEPSLERPSPTRPLHRQTTWAGRSLRDPASPSGRLVKSGSLGSAQGAQPTVEAGVAHMIEALGVLKPRGSPVPWHDGSLSDLSLTGEEPAPGGSPGDSGSALSTQSSEALEGLSGRVPKASGHQDEASTPRRGLGARLQQLLTPSRRSPTSRIPPPELPPDLPPPARRSPMDSLLRPRERPGSTASESSASLGSEWDLSESSVSSLSLRHSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDFRPSAPSPKPAPADAGGNKDAPVPGGPGPVLSNRRLCLALDEPQLCNGHMGGASRRVEGGAWAYLSPLVLRKELESLIENEGSEVLALPELPATHPIIFWNLLWYFQRLQLPSILPCLVLASCDGPPPPQAPSPWLTPDPASVQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAAMGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_014739909.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014739909.1

ORF Insert Sequence:

ATGCCCTGGG CATTACTGAC CTCTGCCTGG TGCTGCCCAG CAAGGGCGAG GGCACCCCTC 
ACACGTACTG CCGATTGCCC CGCAACCTCA ACCCTGGCAT GTGGGGCCCA GCGATGTACC 
TGTGCTACAA GGTGGGCCTG GCCAAGGCCA ACACTCTGGT GTATGAGGCA GAACTGCTGG 
GCCGCTACCC AGAGGAGGAC AATGAGGCGT TCCCGCTGCC CGAGTCCGTG CCGGTCTTCT 
GCCTGCCCAT GGGGGCCACT GTCGAGTGCT GGCCCGCCCA GACCAAGTAC CCCGTACCCG 
TCTTCTCCAC CTTTGTGCTC ACGGGCGCAG CTGGTGATAA GGCACATCTC CCACTTCATT 
CACAACGTCC CCTTCCCTTC CCCACAGAGA CCCCGCATCC TGGTGCAGAT GTCCCCGTAT 
GACAACCTGC TCCTCTGCCA GCCTGTGTCC TCACCCTTGC CCCTCAGTGG TGCCAGCTTC 
CTGCAGCTGC TGCAGAGCCT GGGCCCTGAG CTGGCTATCA CGCTGCTGCT GGCTGTGCTC 
ACAGAGCACA AACTGCTAGT CCACTCGCTG CGGCCGGACC TGCTCACCAG TGTCTGCGAG 
GCCCTCGTCT CTATGATCTT CCCGCTGCAC TGGCAGTGCC CCTACATTCC ACTGTGCCCA 
CTGGTGCTGG CAGATGTGCT GAGCGCCCCC GTGCCCTTCA TTGTGGGTAT CCACTCCAGC 
TACTTCGATC TGCATGACCC TCCTGCTGAT GTCATCTGTG TTGATCTTGA TACCAACACA 
CTCTTCCAGA CTGAGGAAAA GAAGCCCCTC TCCCCTCGGA CTCTGCCCCG CAGACCCTAC 
AAAGTTCTGT TGGCTACACT GACAAACCTA TATCAGCAGC TGGATCAGAC GTATACTGGA 
CCCGAGGAGG AGGCATCCCT GGAATTCCTG CTGACAGACT ACGAGGCAGT GTGCGGCCGC 
CGGGCCCGGC TGGAGCGTGA GGTCCAGGGA GCCTTCCTCC GCTTCATGGC CTGTCTGCTC 
AAGGGCTACC GGGACTTCCT GCTCCCGCTC ACCCAGGCCC CCTCGGAGGG GGCTCGTGAT 
GTTGACAACC TGTTCTTCCT GCAGGGCTTC CTCAAGTCCC GGGAACGCTC CAGCCATAAG 
CTGTACTCTC AGCTGCTACG CACACAGATG TTCTCGCAGT TCATCGAGGA ATGCTCTTTT 
GGCTCTGCTC GGCATGCTGC CCTTGAATTC TTTGACTCTT GTGTTGACAA GGTCCACCCA 
GAGCAGGAGA AGCCTGAACC GACCCCCCTA GTGGAGCTGG AGGAGCTGTC GGGAAGTGAG 
CTCACTGTGT TTATCACACC TCCTGAGGAG CCTCCAGTGC CAGAGGGCAG TGAACCCACT 
CCCCAGTACT GCTATGATGG GTTCCCAGAG CTACAGGCTG AGCTGTTTGA GTCTCCTCAA 
GAGCAGCCTG GGGCTCTGCC TGTGCCGGGC CCCTCCCGTA GTGCCCCCAG CAGTCCTGCC 
CCACGCCGTA CCAAACAGGA GATGAAGGTC GCACAACGGA TGGCGCAGAA GTCAGCAGCT 
GTGCCTGAGC TGTGGGCCCG GTGCCTTCTG GGGCACTGCT ACGGGCTGTG GTTCCTGTGT 
CTGCCTGCTT ACGTGCGCTC GGCGCCCTCC CGGGTGCGGG CACTGCACAC GGCCTACCAA 
GTGCTGCGCC AGATGGAGAG CCGCAAGGTG GTGCTGCCCG ATGAGGTGTG TTACCGGGTG 
CTGATGCAGC TCTGCTCACA CTATGGGCAG CCAGTGCTGT CTGTTCAGGT CATGCTAGAG 
ATGCTGCGAG CAGGCATTGT GCCCAACACC ATCACCTACG GCTACTACAA CAAGGCTGTG 
CTGGAAAGCA AGTGGCCGTC TGGTACACCG GGTGGGCGCC TGCGCTGGGC CAAGCTCCGG 
AACGTCGTCC TGGGGGCTGC TCAGTTCCGC CAGCCCTTGA GAGAACGGCG GAAGCAGCGG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG AAGCAGCAGG CAGCAGCACA ACAAAAGACA 
GGCAGCTCCC AGACAGAGCC CTCTCTGGAG CGCCCCTCCC CAACCCGCCC CCTTCATCGC 
CAGACTACTT GGGCTGGGCG AAGCCTGCGG GACCCAGCCT CACCCTCAGG GCGCCTGGTG 
AAGAGTGGCA GCCTGGGCAG TGCCCAAGGG GCACAGCCCA CTGTGGAGGC TGGCGTAGCC 
CACATGATAG AGGCCTTGGG GGTATTGAAA CCCCGAGGAT CACCTGTGCC CTGGCACGAT 
GGAAGCCTTT CAGACTTGAG CCTGACCGGG GAGGAGCCAG CACCTGGAGG AAGCCCAGGG 
GACTCTGGCT CAGCCCTGAG TACTCAGTCC AGTGAAGCCC TGGAAGGGCT AAGTGGCCGG 
GTACCCAAGG CTAGTGGGCA TCAGGATGAG GCCAGCACCC CCCGACGAGG GCTGGGTGCC 
CGCCTCCAAC AGCTGCTCAC TCCTTCCCGC CGCTCCCCGA CCTCTCGCAT TCCCCCACCT 
GAGCTGCCCC CTGACCTGCC TCCCCCAGCC CGCCGCAGCC CCATGGACAG CCTTCTGCGC 
CCCCGGGAGC GCCCTGGATC CACTGCCTCT GAGAGCTCAG CCTCTCTGGG CAGTGAGTGG 
GACCTCTCAG AATCTTCCGT CAGCAGCCTG AGCCTTCGCC ATTCCTCAGA GCGCCTCAGT 
GACACCCCTG GATCCTTCCA GCCACCTTCC CTGGAAATTC TGCTGTCTAG TTGCTCCTTG 
TGCCGCGCCT GTGATTCGCT CGTGTACGAT GAGGAGATCA TGGCTGGCTG GGCACCTGAT 
GACTCCAACC TCAACACAAC CTGCCCCTTC TGCGCATGCC CCTTTGTGCC CCTGCTCAGT 
GTCCAGACCC TTGATTTCCG ACCCAGTGCC CCCAGCCCCA AGCCTGCCCC TGCGGATGCC 
GGAGGCAACA AAGACGCACC TGTCCCTGGG GGCCCAGGAC CTGTGCTCAG TAACCGCAGG 
CTCTGCCTTG CCCTGGATGA GCCCCAGCTC TGCAATGGGC ACATGGGGGG TGCCTCCCGG 
CGGGTCGAGG GTGGGGCATG GGCATACCTG AGCCCCCTGG TGCTGCGTAA GGAGTTGGAG 
TCGCTGATAG AGAACGAGGG CAGCGAGGTG CTGGCATTGC CTGAGCTGCC TGCTACCCAC 
CCCATTATCT TCTGGAACCT TCTGTGGTAT TTCCAGCGAC TGCAACTGCC CAGTATTCTG 
CCATGCCTGG TGCTGGCCTC CTGTGATGGG CCCCCACCCC CCCAGGCCCC ATCTCCTTGG 
CTAACACCTG ATCCAGCCTC TGTGCAGGTG CGGCTGCTGT GGGATGTCCT GACCCCCGAC 
CCCAATAGCT GCCCACCTCT GTATGTGCTC TGGAGAGTCC ACAGCCAGAT CCCCCAGCGG 
GTAGTATGGC CAGGCCCAGT ACCTGCCTCC CTTAGCCTGG CGTTGCTGGA GTCGGTGCTG 
CGCCATGTTG GTCTCAATGA GGTGCACAAG GCTGTAGGGC TCCTGCTGGA AACTCTAGGG 
CCTCCTCCCA CAGGCCTGCA CCTACAGAGG GGCATCTACC GTGAGATCTT ATTCCTGACA 
ATGGCTGCTA TGGGCAAGGA CCACATGGAC ATAGTGGCCT TCGACAAGAA GTACAAGTCT 
GCCTTTAACA AGCTGGCCAG CAGCATGGGC AAGGAGGAGC TGAGGCAGCG GCGGGCACAG 
ATGCCCACTC CAAAGGCCAT TGATTGCCGA AAATGTTTTG GAGCACCTCT GGAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_023641110.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023496878.1 Equus caballus DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.

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CloneID	OHb65322
Clone ID Related Accession (Same CDS sequence)	XM_023641110.1 , XM_023641111.1
Accession Version	XM_023641110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AGCGGCCCCC CCGGCTCGTG GATTACTTCG TGATAGCTGG GCTTGCAGGG 
AACGGAGCAC CTATCCCTGA GGAAACGTGG GTCCCTGAAC CCAGTGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCTGCTGGGG GCCACCCCTT GGAACTCAGT 
GCTGGGCTTC TGGGAGGAAC TCAACCTGTC ATCTGCTACC GCAGGGGCCG TGACAAACCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGACA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG ACCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATTACTGACC TCTGCCTGGT GCTGCCCAGC AAGGGCGAGG GCACCCCTCA CACGTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CGATGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACTCTGGTG TATGAGGCAG AACTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCCGTGC CGGTCTTCTG CCTGCCCATG 
GGGGCCACTG TCGAGTGCTG GCCCGCCCAG ACCAAGTACC CCGTACCCGT CTTCTCCACC 
TTTGTGCTCA CGGGCGCAGC TGGTGATAAG GTGTACGGCG CCGCCCTGCA GTTCTACGAG 
GCGTTCCCGC GGGCCAGGCT GTCGGAGCGG CAGGCGCGGG CGCTGGGCCT GCTGAGCGCG 
GTGGAGCGCG GCCGCGCGCT GGGGGGCCGG GCCGTGCGCA GCCGCCGCGC CATCGCCGTG 
CTGTCCCGCT GGCCTGCCTT CCCTGCCTTC CGCGCTTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCAG GCCCCCACCG CCTACCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCCCC GTATGACAAC 
CTGCTCCTCT GCCAGCCTGT GTCCTCACCC TTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA GCCTGGGCCC TGAGCTGGCT ATCACGCTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TAGTCCACTC GCTGCGGCCG GACCTGCTCA CCAGTGTCTG CGAGGCCCTC 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCACTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGCGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCTCCTGC TGATGTCATC TGTGTTGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACTCTGC CCCGCAGACC CTACAAAGTT 
CTGTTGGCTA CACTGACAAA CCTATATCAG CAGCTGGATC AGACGTATAC TGGACCCGAG 
GAGGAGGCAT CCCTGGAATT CCTGCTGACA GACTACGAGG CAGTGTGCGG CCGCCGGGCC 
CGGCTGGAGC GTGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGTCT GCTCAAGGGC 
TACCGGGACT TCCTGCTCCC GCTCACCCAG GCCCCCTCGG AGGGGGCTCG TGATGTTGAC 
AACCTGTTCT TCCTGCAGGG CTTCCTCAAG TCCCGGGAAC GCTCCAGCCA TAAGCTGTAC 
TCTCAGCTGC TACGCACACA GATGTTCTCG CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTTG ACAAGGTCCA CCCAGAGCAG 
GAGAAGCCTG AACCGACCCC CCTAGTGGAG CTGGAGGAGC TGTCGGGAAG TGAGCTCACT 
GTGTTTATCA CACCTCCTGA GGAGCCTCCA GTGCCAGAGG GCAGTGAACC CACTCCCCAG 
TACTGCTATG ATGGGTTCCC AGAGCTACAG GCTGAGCTGT TTGAGTCTCC TCAAGAGCAG 
CCTGGGGCTC TGCCTGTGCC GGGCCCCTCC CGTAGTGCCC CCAGCAGTCC TGCCCCACGC 
CGTACCAAAC AGGAGATGAA GGTCGCACAA CGGATGGCGC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT TCTGGGGCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCTTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC ACACGGCCTA CCAAGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCCGATGAGG TGTGTTACCG GGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCAGTG CTGTCTGTTC AGGTCATGCT AGAGATGCTG 
CGAGCAGGCA TTGTGCCCAA CACCATCACC TACGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGTAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTC 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGAGAGAAC GGCGGAAGCA GCGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGAAGCAG CAGGCAGCAG CACAACAAAA GACAGGCAGC 
TCCCAGACAG AGCCCTCTCT GGAGCGCCCC TCCCCAACCC GCCCCCTTCA TCGCCAGACT 
ACTTGGGCTG GGCGAAGCCT GCGGGACCCA GCCTCACCCT CAGGGCGCCT GGTGAAGAGT 
GGCAGCCTGG GCAGTGCCCA AGGGGCACAG CCCACTGTGG AGGCTGGCGT AGCCCACATG 
ATAGAGGCCT TGGGGGTATT GAAACCCCGA GGATCACCTG TGCCCTGGCA CGATGGAAGC 
CTTTCAGACT TGAGCCTGAC CGGGGAGGAG CCAGCACCTG GAGGAAGCCC AGGGGACTCT 
GGCTCAGCCC TGAGTACTCA GTCCAGTGAA GCCCTGGAAG GGCTAAGTGG CCGGGTACCC 
AAGGCTAGTG GGCATCAGGA TGAGGCCAGC ACCCCCCGAC GAGGGCTGGG TGCCCGCCTC 
CAACAGCTGC TCACTCCTTC CCGCCGCTCC CCGACCTCTC GCATTCCCCC ACCTGAGCTG 
CCCCCTGACC TGCCTCCCCC AGCCCGCCGC AGCCCCATGG ACAGCCTTCT GCGCCCCCGG 
GAGCGCCCTG GATCCACTGC CTCTGAGAGC TCAGCCTCTC TGGGCAGTGA GTGGGACCTC 
TCAGAATCTT CCGTCAGCAG CCTGAGCCTT CGCCATTCCT CAGAGCGCCT CAGTGACACC 
CCTGGATCCT TCCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGTTGCTC CTTGTGCCGC 
GCCTGTGATT CGCTCGTGTA CGATGAGGAG ATCATGGCTG GCTGGGCACC TGATGACTCC 
AACCTCAACA CAACCTGCCC CTTCTGCGCA TGCCCCTTTG TGCCCCTGCT CAGTGTCCAG 
ACCCTTGATT TCCGACCCAG TGCCCCCAGC CCCAAGCCTG CCCCTGCGGA TGCCGGAGGC 
AACAAAGACG CACCTGTCCC TGGGGGCCCA GGACCTGTGC TCAGTAACCG CAGGCTCTGC 
CTTGCCCTGG ATGAGCCCCA GCTCTGCAAT GGGCACATGG GGGGTGCCTC CCGGCGGGTC 
GAGGGTGGGG CATGGGCATA CCTGAGCCCC CTGGTGCTGC GTAAGGAGTT GGAGTCGCTG 
ATAGAGAACG AGGGCAGCGA GGTGCTGGCA TTGCCTGAGC TGCCTGCTAC CCACCCCATT 
ATCTTCTGGA ACCTTCTGTG GTATTTCCAG CGACTGCAAC TGCCCAGTAT TCTGCCATGC 
CTGGTGCTGG CCTCCTGTGA TGGGCCCCCA CCCCCCCAGG CCCCATCTCC TTGGCTAACA 
CCTGATCCAG CCTCTGTGCA GGTGCGGCTG CTGTGGGATG TCCTGACCCC CGACCCCAAT 
AGCTGCCCAC CTCTGTATGT GCTCTGGAGA GTCCACAGCC AGATCCCCCA GCGGGTAGTA 
TGGCCAGGCC CAGTACCTGC CTCCCTTAGC CTGGCGTTGC TGGAGTCGGT GCTGCGCCAT 
GTTGGTCTCA ATGAGGTGCA CAAGGCTGTA GGGCTCCTGC TGGAAACTCT AGGGCCTCCT 
CCCACAGGCC TGCACCTACA GAGGGGCATC TACCGTGAGA TCTTATTCCT GACAATGGCT 
GCTATGGGCA AGGACCACAT GGACATAGTG GCCTTCGACA AGAAGTACAA GTCTGCCTTT 
AACAAGCTGG CCAGCAGCAT GGGCAAGGAG GAGCTGAGGC AGCGGCGGGC ACAGATGCCC 
ACTCCAAAGG CCATTGATTG CCGAAAATGT TTTGGAGCAC CTCTGGAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023496878.1
CDS	408..4901
Translation	MAEERPPRLVDYFVIAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAMYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATVECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLLPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLRTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVPEGSEPTPQYCYDGFPELQAELFESPQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVQVMLEMLRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERRKQRQQQQQQQQQQKQQAAAQQKTGSSQTEPSLERPSPTRPLHRQTTWAGRSLRDPASPSGRLVKSGSLGSAQGAQPTVEAGVAHMIEALGVLKPRGSPVPWHDGSLSDLSLTGEEPAPGGSPGDSGSALSTQSSEALEGLSGRVPKASGHQDEASTPRRGLGARLQQLLTPSRRSPTSRIPPPELPPDLPPPARRSPMDSLLRPRERPGSTASESSASLGSEWDLSESSVSSLSLRHSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDFRPSAPSPKPAPADAGGNKDAPVPGGPGPVLSNRRLCLALDEPQLCNGHMGGASRRVEGGAWAYLSPLVLRKELESLIENEGSEVLALPELPATHPIIFWNLLWYFQRLQLPSILPCLVLASCDGPPPPQAPSPWLTPDPASVQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAAMGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_023641110.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023641110.1

ORF Insert Sequence:

ATGGCGGAGG AGCGGCCCCC CCGGCTCGTG GATTACTTCG TGATAGCTGG GCTTGCAGGG 
AACGGAGCAC CTATCCCTGA GGAAACGTGG GTCCCTGAAC CCAGTGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCTGCTGGGG GCCACCCCTT GGAACTCAGT 
GCTGGGCTTC TGGGAGGAAC TCAACCTGTC ATCTGCTACC GCAGGGGCCG TGACAAACCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGACA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG ACCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATTACTGACC TCTGCCTGGT GCTGCCCAGC AAGGGCGAGG GCACCCCTCA CACGTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CGATGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACTCTGGTG TATGAGGCAG AACTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCCGTGC CGGTCTTCTG CCTGCCCATG 
GGGGCCACTG TCGAGTGCTG GCCCGCCCAG ACCAAGTACC CCGTACCCGT CTTCTCCACC 
TTTGTGCTCA CGGGCGCAGC TGGTGATAAG GTGTACGGCG CCGCCCTGCA GTTCTACGAG 
GCGTTCCCGC GGGCCAGGCT GTCGGAGCGG CAGGCGCGGG CGCTGGGCCT GCTGAGCGCG 
GTGGAGCGCG GCCGCGCGCT GGGGGGCCGG GCCGTGCGCA GCCGCCGCGC CATCGCCGTG 
CTGTCCCGCT GGCCTGCCTT CCCTGCCTTC CGCGCTTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCAG GCCCCCACCG CCTACCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCCCC GTATGACAAC 
CTGCTCCTCT GCCAGCCTGT GTCCTCACCC TTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA GCCTGGGCCC TGAGCTGGCT ATCACGCTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TAGTCCACTC GCTGCGGCCG GACCTGCTCA CCAGTGTCTG CGAGGCCCTC 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCACTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGCGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCTCCTGC TGATGTCATC TGTGTTGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACTCTGC CCCGCAGACC CTACAAAGTT 
CTGTTGGCTA CACTGACAAA CCTATATCAG CAGCTGGATC AGACGTATAC TGGACCCGAG 
GAGGAGGCAT CCCTGGAATT CCTGCTGACA GACTACGAGG CAGTGTGCGG CCGCCGGGCC 
CGGCTGGAGC GTGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGTCT GCTCAAGGGC 
TACCGGGACT TCCTGCTCCC GCTCACCCAG GCCCCCTCGG AGGGGGCTCG TGATGTTGAC 
AACCTGTTCT TCCTGCAGGG CTTCCTCAAG TCCCGGGAAC GCTCCAGCCA TAAGCTGTAC 
TCTCAGCTGC TACGCACACA GATGTTCTCG CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTTG ACAAGGTCCA CCCAGAGCAG 
GAGAAGCCTG AACCGACCCC CCTAGTGGAG CTGGAGGAGC TGTCGGGAAG TGAGCTCACT 
GTGTTTATCA CACCTCCTGA GGAGCCTCCA GTGCCAGAGG GCAGTGAACC CACTCCCCAG 
TACTGCTATG ATGGGTTCCC AGAGCTACAG GCTGAGCTGT TTGAGTCTCC TCAAGAGCAG 
CCTGGGGCTC TGCCTGTGCC GGGCCCCTCC CGTAGTGCCC CCAGCAGTCC TGCCCCACGC 
CGTACCAAAC AGGAGATGAA GGTCGCACAA CGGATGGCGC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT TCTGGGGCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCTTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC ACACGGCCTA CCAAGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCCGATGAGG TGTGTTACCG GGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCAGTG CTGTCTGTTC AGGTCATGCT AGAGATGCTG 
CGAGCAGGCA TTGTGCCCAA CACCATCACC TACGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGTAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTC 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGAGAGAAC GGCGGAAGCA GCGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGAAGCAG CAGGCAGCAG CACAACAAAA GACAGGCAGC 
TCCCAGACAG AGCCCTCTCT GGAGCGCCCC TCCCCAACCC GCCCCCTTCA TCGCCAGACT 
ACTTGGGCTG GGCGAAGCCT GCGGGACCCA GCCTCACCCT CAGGGCGCCT GGTGAAGAGT 
GGCAGCCTGG GCAGTGCCCA AGGGGCACAG CCCACTGTGG AGGCTGGCGT AGCCCACATG 
ATAGAGGCCT TGGGGGTATT GAAACCCCGA GGATCACCTG TGCCCTGGCA CGATGGAAGC 
CTTTCAGACT TGAGCCTGAC CGGGGAGGAG CCAGCACCTG GAGGAAGCCC AGGGGACTCT 
GGCTCAGCCC TGAGTACTCA GTCCAGTGAA GCCCTGGAAG GGCTAAGTGG CCGGGTACCC 
AAGGCTAGTG GGCATCAGGA TGAGGCCAGC ACCCCCCGAC GAGGGCTGGG TGCCCGCCTC 
CAACAGCTGC TCACTCCTTC CCGCCGCTCC CCGACCTCTC GCATTCCCCC ACCTGAGCTG 
CCCCCTGACC TGCCTCCCCC AGCCCGCCGC AGCCCCATGG ACAGCCTTCT GCGCCCCCGG 
GAGCGCCCTG GATCCACTGC CTCTGAGAGC TCAGCCTCTC TGGGCAGTGA GTGGGACCTC 
TCAGAATCTT CCGTCAGCAG CCTGAGCCTT CGCCATTCCT CAGAGCGCCT CAGTGACACC 
CCTGGATCCT TCCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGTTGCTC CTTGTGCCGC 
GCCTGTGATT CGCTCGTGTA CGATGAGGAG ATCATGGCTG GCTGGGCACC TGATGACTCC 
AACCTCAACA CAACCTGCCC CTTCTGCGCA TGCCCCTTTG TGCCCCTGCT CAGTGTCCAG 
ACCCTTGATT TCCGACCCAG TGCCCCCAGC CCCAAGCCTG CCCCTGCGGA TGCCGGAGGC 
AACAAAGACG CACCTGTCCC TGGGGGCCCA GGACCTGTGC TCAGTAACCG CAGGCTCTGC 
CTTGCCCTGG ATGAGCCCCA GCTCTGCAAT GGGCACATGG GGGGTGCCTC CCGGCGGGTC 
GAGGGTGGGG CATGGGCATA CCTGAGCCCC CTGGTGCTGC GTAAGGAGTT GGAGTCGCTG 
ATAGAGAACG AGGGCAGCGA GGTGCTGGCA TTGCCTGAGC TGCCTGCTAC CCACCCCATT 
ATCTTCTGGA ACCTTCTGTG GTATTTCCAG CGACTGCAAC TGCCCAGTAT TCTGCCATGC 
CTGGTGCTGG CCTCCTGTGA TGGGCCCCCA CCCCCCCAGG CCCCATCTCC TTGGCTAACA 
CCTGATCCAG CCTCTGTGCA GGTGCGGCTG CTGTGGGATG TCCTGACCCC CGACCCCAAT 
AGCTGCCCAC CTCTGTATGT GCTCTGGAGA GTCCACAGCC AGATCCCCCA GCGGGTAGTA 
TGGCCAGGCC CAGTACCTGC CTCCCTTAGC CTGGCGTTGC TGGAGTCGGT GCTGCGCCAT 
GTTGGTCTCA ATGAGGTGCA CAAGGCTGTA GGGCTCCTGC TGGAAACTCT AGGGCCTCCT 
CCCACAGGCC TGCACCTACA GAGGGGCATC TACCGTGAGA TCTTATTCCT GACAATGGCT 
GCTATGGGCA AGGACCACAT GGACATAGTG GCCTTCGACA AGAAGTACAA GTCTGCCTTT 
AACAAGCTGG CCAGCAGCAT GGGCAAGGAG GAGCTGAGGC AGCGGCGGGC ACAGATGCCC 
ACTCCAAAGG CCATTGATTG CCGAAAATGT TTTGGAGCAC CTCTGGAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_023641111.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023496879.1 Equus caballus DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.

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CloneID	OHb65322
Clone ID Related Accession (Same CDS sequence)	XM_023641110.1 , XM_023641111.1
Accession Version	XM_023641111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	DENN domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG AGCGGCCCCC CCGGCTCGTG GATTACTTCG TGATAGCTGG GCTTGCAGGG 
AACGGAGCAC CTATCCCTGA GGAAACGTGG GTCCCTGAAC CCAGTGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCTGCTGGGG GCCACCCCTT GGAACTCAGT 
GCTGGGCTTC TGGGAGGAAC TCAACCTGTC ATCTGCTACC GCAGGGGCCG TGACAAACCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGACA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG ACCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATTACTGACC TCTGCCTGGT GCTGCCCAGC AAGGGCGAGG GCACCCCTCA CACGTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CGATGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACTCTGGTG TATGAGGCAG AACTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCCGTGC CGGTCTTCTG CCTGCCCATG 
GGGGCCACTG TCGAGTGCTG GCCCGCCCAG ACCAAGTACC CCGTACCCGT CTTCTCCACC 
TTTGTGCTCA CGGGCGCAGC TGGTGATAAG GTGTACGGCG CCGCCCTGCA GTTCTACGAG 
GCGTTCCCGC GGGCCAGGCT GTCGGAGCGG CAGGCGCGGG CGCTGGGCCT GCTGAGCGCG 
GTGGAGCGCG GCCGCGCGCT GGGGGGCCGG GCCGTGCGCA GCCGCCGCGC CATCGCCGTG 
CTGTCCCGCT GGCCTGCCTT CCCTGCCTTC CGCGCTTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCAG GCCCCCACCG CCTACCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCCCC GTATGACAAC 
CTGCTCCTCT GCCAGCCTGT GTCCTCACCC TTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA GCCTGGGCCC TGAGCTGGCT ATCACGCTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TAGTCCACTC GCTGCGGCCG GACCTGCTCA CCAGTGTCTG CGAGGCCCTC 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCACTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGCGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCTCCTGC TGATGTCATC TGTGTTGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACTCTGC CCCGCAGACC CTACAAAGTT 
CTGTTGGCTA CACTGACAAA CCTATATCAG CAGCTGGATC AGACGTATAC TGGACCCGAG 
GAGGAGGCAT CCCTGGAATT CCTGCTGACA GACTACGAGG CAGTGTGCGG CCGCCGGGCC 
CGGCTGGAGC GTGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGTCT GCTCAAGGGC 
TACCGGGACT TCCTGCTCCC GCTCACCCAG GCCCCCTCGG AGGGGGCTCG TGATGTTGAC 
AACCTGTTCT TCCTGCAGGG CTTCCTCAAG TCCCGGGAAC GCTCCAGCCA TAAGCTGTAC 
TCTCAGCTGC TACGCACACA GATGTTCTCG CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTTG ACAAGGTCCA CCCAGAGCAG 
GAGAAGCCTG AACCGACCCC CCTAGTGGAG CTGGAGGAGC TGTCGGGAAG TGAGCTCACT 
GTGTTTATCA CACCTCCTGA GGAGCCTCCA GTGCCAGAGG GCAGTGAACC CACTCCCCAG 
TACTGCTATG ATGGGTTCCC AGAGCTACAG GCTGAGCTGT TTGAGTCTCC TCAAGAGCAG 
CCTGGGGCTC TGCCTGTGCC GGGCCCCTCC CGTAGTGCCC CCAGCAGTCC TGCCCCACGC 
CGTACCAAAC AGGAGATGAA GGTCGCACAA CGGATGGCGC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT TCTGGGGCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCTTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC ACACGGCCTA CCAAGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCCGATGAGG TGTGTTACCG GGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCAGTG CTGTCTGTTC AGGTCATGCT AGAGATGCTG 
CGAGCAGGCA TTGTGCCCAA CACCATCACC TACGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGTAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTC 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGAGAGAAC GGCGGAAGCA GCGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGAAGCAG CAGGCAGCAG CACAACAAAA GACAGGCAGC 
TCCCAGACAG AGCCCTCTCT GGAGCGCCCC TCCCCAACCC GCCCCCTTCA TCGCCAGACT 
ACTTGGGCTG GGCGAAGCCT GCGGGACCCA GCCTCACCCT CAGGGCGCCT GGTGAAGAGT 
GGCAGCCTGG GCAGTGCCCA AGGGGCACAG CCCACTGTGG AGGCTGGCGT AGCCCACATG 
ATAGAGGCCT TGGGGGTATT GAAACCCCGA GGATCACCTG TGCCCTGGCA CGATGGAAGC 
CTTTCAGACT TGAGCCTGAC CGGGGAGGAG CCAGCACCTG GAGGAAGCCC AGGGGACTCT 
GGCTCAGCCC TGAGTACTCA GTCCAGTGAA GCCCTGGAAG GGCTAAGTGG CCGGGTACCC 
AAGGCTAGTG GGCATCAGGA TGAGGCCAGC ACCCCCCGAC GAGGGCTGGG TGCCCGCCTC 
CAACAGCTGC TCACTCCTTC CCGCCGCTCC CCGACCTCTC GCATTCCCCC ACCTGAGCTG 
CCCCCTGACC TGCCTCCCCC AGCCCGCCGC AGCCCCATGG ACAGCCTTCT GCGCCCCCGG 
GAGCGCCCTG GATCCACTGC CTCTGAGAGC TCAGCCTCTC TGGGCAGTGA GTGGGACCTC 
TCAGAATCTT CCGTCAGCAG CCTGAGCCTT CGCCATTCCT CAGAGCGCCT CAGTGACACC 
CCTGGATCCT TCCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGTTGCTC CTTGTGCCGC 
GCCTGTGATT CGCTCGTGTA CGATGAGGAG ATCATGGCTG GCTGGGCACC TGATGACTCC 
AACCTCAACA CAACCTGCCC CTTCTGCGCA TGCCCCTTTG TGCCCCTGCT CAGTGTCCAG 
ACCCTTGATT TCCGACCCAG TGCCCCCAGC CCCAAGCCTG CCCCTGCGGA TGCCGGAGGC 
AACAAAGACG CACCTGTCCC TGGGGGCCCA GGACCTGTGC TCAGTAACCG CAGGCTCTGC 
CTTGCCCTGG ATGAGCCCCA GCTCTGCAAT GGGCACATGG GGGGTGCCTC CCGGCGGGTC 
GAGGGTGGGG CATGGGCATA CCTGAGCCCC CTGGTGCTGC GTAAGGAGTT GGAGTCGCTG 
ATAGAGAACG AGGGCAGCGA GGTGCTGGCA TTGCCTGAGC TGCCTGCTAC CCACCCCATT 
ATCTTCTGGA ACCTTCTGTG GTATTTCCAG CGACTGCAAC TGCCCAGTAT TCTGCCATGC 
CTGGTGCTGG CCTCCTGTGA TGGGCCCCCA CCCCCCCAGG CCCCATCTCC TTGGCTAACA 
CCTGATCCAG CCTCTGTGCA GGTGCGGCTG CTGTGGGATG TCCTGACCCC CGACCCCAAT 
AGCTGCCCAC CTCTGTATGT GCTCTGGAGA GTCCACAGCC AGATCCCCCA GCGGGTAGTA 
TGGCCAGGCC CAGTACCTGC CTCCCTTAGC CTGGCGTTGC TGGAGTCGGT GCTGCGCCAT 
GTTGGTCTCA ATGAGGTGCA CAAGGCTGTA GGGCTCCTGC TGGAAACTCT AGGGCCTCCT 
CCCACAGGCC TGCACCTACA GAGGGGCATC TACCGTGAGA TCTTATTCCT GACAATGGCT 
GCTATGGGCA AGGACCACAT GGACATAGTG GCCTTCGACA AGAAGTACAA GTCTGCCTTT 
AACAAGCTGG CCAGCAGCAT GGGCAAGGAG GAGCTGAGGC AGCGGCGGGC ACAGATGCCC 
ACTCCAAAGG CCATTGATTG CCGAAAATGT TTTGGAGCAC CTCTGGAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023496879.1
CDS	130..4623
Translation	MAEERPPRLVDYFVIAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAMYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATVECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLLPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLRTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVPEGSEPTPQYCYDGFPELQAELFESPQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVQVMLEMLRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERRKQRQQQQQQQQQQKQQAAAQQKTGSSQTEPSLERPSPTRPLHRQTTWAGRSLRDPASPSGRLVKSGSLGSAQGAQPTVEAGVAHMIEALGVLKPRGSPVPWHDGSLSDLSLTGEEPAPGGSPGDSGSALSTQSSEALEGLSGRVPKASGHQDEASTPRRGLGARLQQLLTPSRRSPTSRIPPPELPPDLPPPARRSPMDSLLRPRERPGSTASESSASLGSEWDLSESSVSSLSLRHSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDFRPSAPSPKPAPADAGGNKDAPVPGGPGPVLSNRRLCLALDEPQLCNGHMGGASRRVEGGAWAYLSPLVLRKELESLIENEGSEVLALPELPATHPIIFWNLLWYFQRLQLPSILPCLVLASCDGPPPPQAPSPWLTPDPASVQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAAMGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_023641111.1
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023641111.1

ORF Insert Sequence:

ATGGCGGAGG AGCGGCCCCC CCGGCTCGTG GATTACTTCG TGATAGCTGG GCTTGCAGGG 
AACGGAGCAC CTATCCCTGA GGAAACGTGG GTCCCTGAAC CCAGTGGGCC CCTGCGCCCT 
CCCCGGCCAG CTGAGCCCAT CACAGATGTG GCAGTCATCG CTAGGGCACT GGGCGAGGAG 
GTGCCCCAGG GCTACACATG CATCCAGGCT TCTGCTGGGG GCCACCCCTT GGAACTCAGT 
GCTGGGCTTC TGGGAGGAAC TCAACCTGTC ATCTGCTACC GCAGGGGCCG TGACAAACCC 
CCCCTCGTTG AGCTGGGGGT GTTGTATGAG GGGAAGGAAC GTCCCAAGCC TGGCTTCCAA 
GTGCTAGACA CGACACCCTA CAGCCACTCA GCCAACCTGG CCCCTCCAGG ACCTGGGCAC 
CCCCGCACCT ACCTCACTTA CCGGCGGGCA GCAGAAGGGG CAGGGCTGCA TGCCCTGGGC 
ATTACTGACC TCTGCCTGGT GCTGCCCAGC AAGGGCGAGG GCACCCCTCA CACGTACTGC 
CGATTGCCCC GCAACCTCAA CCCTGGCATG TGGGGCCCAG CGATGTACCT GTGCTACAAG 
GTGGGCCTGG CCAAGGCCAA CACTCTGGTG TATGAGGCAG AACTGCTGGG CCGCTACCCA 
GAGGAGGACA ATGAGGCGTT CCCGCTGCCC GAGTCCGTGC CGGTCTTCTG CCTGCCCATG 
GGGGCCACTG TCGAGTGCTG GCCCGCCCAG ACCAAGTACC CCGTACCCGT CTTCTCCACC 
TTTGTGCTCA CGGGCGCAGC TGGTGATAAG GTGTACGGCG CCGCCCTGCA GTTCTACGAG 
GCGTTCCCGC GGGCCAGGCT GTCGGAGCGG CAGGCGCGGG CGCTGGGCCT GCTGAGCGCG 
GTGGAGCGCG GCCGCGCGCT GGGGGGCCGG GCCGTGCGCA GCCGCCGCGC CATCGCCGTG 
CTGTCCCGCT GGCCTGCCTT CCCTGCCTTC CGCGCTTTCC TCACCTTCCT CTACCGCTAC 
TCCGTCTCAG GCCCCCACCG CCTACCCTTG GAAGCGCACA TCTCCCACTT CATTCACAAC 
GTCCCCTTCC CTTCCCCACA GAGACCCCGC ATCCTGGTGC AGATGTCCCC GTATGACAAC 
CTGCTCCTCT GCCAGCCTGT GTCCTCACCC TTGCCCCTCA GTGGTGCCAG CTTCCTGCAG 
CTGCTGCAGA GCCTGGGCCC TGAGCTGGCT ATCACGCTGC TGCTGGCTGT GCTCACAGAG 
CACAAACTGC TAGTCCACTC GCTGCGGCCG GACCTGCTCA CCAGTGTCTG CGAGGCCCTC 
GTCTCTATGA TCTTCCCGCT GCACTGGCAG TGCCCCTACA TTCCACTGTG CCCACTGGTG 
CTGGCAGATG TGCTGAGCGC CCCCGTGCCC TTCATTGTGG GTATCCACTC CAGCTACTTC 
GATCTGCATG ACCCTCCTGC TGATGTCATC TGTGTTGATC TTGATACCAA CACACTCTTC 
CAGACTGAGG AAAAGAAGCC CCTCTCCCCT CGGACTCTGC CCCGCAGACC CTACAAAGTT 
CTGTTGGCTA CACTGACAAA CCTATATCAG CAGCTGGATC AGACGTATAC TGGACCCGAG 
GAGGAGGCAT CCCTGGAATT CCTGCTGACA GACTACGAGG CAGTGTGCGG CCGCCGGGCC 
CGGCTGGAGC GTGAGGTCCA GGGAGCCTTC CTCCGCTTCA TGGCCTGTCT GCTCAAGGGC 
TACCGGGACT TCCTGCTCCC GCTCACCCAG GCCCCCTCGG AGGGGGCTCG TGATGTTGAC 
AACCTGTTCT TCCTGCAGGG CTTCCTCAAG TCCCGGGAAC GCTCCAGCCA TAAGCTGTAC 
TCTCAGCTGC TACGCACACA GATGTTCTCG CAGTTCATCG AGGAATGCTC TTTTGGCTCT 
GCTCGGCATG CTGCCCTTGA ATTCTTTGAC TCTTGTGTTG ACAAGGTCCA CCCAGAGCAG 
GAGAAGCCTG AACCGACCCC CCTAGTGGAG CTGGAGGAGC TGTCGGGAAG TGAGCTCACT 
GTGTTTATCA CACCTCCTGA GGAGCCTCCA GTGCCAGAGG GCAGTGAACC CACTCCCCAG 
TACTGCTATG ATGGGTTCCC AGAGCTACAG GCTGAGCTGT TTGAGTCTCC TCAAGAGCAG 
CCTGGGGCTC TGCCTGTGCC GGGCCCCTCC CGTAGTGCCC CCAGCAGTCC TGCCCCACGC 
CGTACCAAAC AGGAGATGAA GGTCGCACAA CGGATGGCGC AGAAGTCAGC AGCTGTGCCT 
GAGCTGTGGG CCCGGTGCCT TCTGGGGCAC TGCTACGGGC TGTGGTTCCT GTGTCTGCCT 
GCTTACGTGC GCTCGGCGCC CTCCCGGGTG CGGGCACTGC ACACGGCCTA CCAAGTGCTG 
CGCCAGATGG AGAGCCGCAA GGTGGTGCTG CCCGATGAGG TGTGTTACCG GGTGCTGATG 
CAGCTCTGCT CACACTATGG GCAGCCAGTG CTGTCTGTTC AGGTCATGCT AGAGATGCTG 
CGAGCAGGCA TTGTGCCCAA CACCATCACC TACGGCTACT ACAACAAGGC TGTGCTGGAA 
AGCAAGTGGC CGTCTGGTAC ACCGGGTGGG CGCCTGCGCT GGGCCAAGCT CCGGAACGTC 
GTCCTGGGGG CTGCTCAGTT CCGCCAGCCC TTGAGAGAAC GGCGGAAGCA GCGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGAAGCAG CAGGCAGCAG CACAACAAAA GACAGGCAGC 
TCCCAGACAG AGCCCTCTCT GGAGCGCCCC TCCCCAACCC GCCCCCTTCA TCGCCAGACT 
ACTTGGGCTG GGCGAAGCCT GCGGGACCCA GCCTCACCCT CAGGGCGCCT GGTGAAGAGT 
GGCAGCCTGG GCAGTGCCCA AGGGGCACAG CCCACTGTGG AGGCTGGCGT AGCCCACATG 
ATAGAGGCCT TGGGGGTATT GAAACCCCGA GGATCACCTG TGCCCTGGCA CGATGGAAGC 
CTTTCAGACT TGAGCCTGAC CGGGGAGGAG CCAGCACCTG GAGGAAGCCC AGGGGACTCT 
GGCTCAGCCC TGAGTACTCA GTCCAGTGAA GCCCTGGAAG GGCTAAGTGG CCGGGTACCC 
AAGGCTAGTG GGCATCAGGA TGAGGCCAGC ACCCCCCGAC GAGGGCTGGG TGCCCGCCTC 
CAACAGCTGC TCACTCCTTC CCGCCGCTCC CCGACCTCTC GCATTCCCCC ACCTGAGCTG 
CCCCCTGACC TGCCTCCCCC AGCCCGCCGC AGCCCCATGG ACAGCCTTCT GCGCCCCCGG 
GAGCGCCCTG GATCCACTGC CTCTGAGAGC TCAGCCTCTC TGGGCAGTGA GTGGGACCTC 
TCAGAATCTT CCGTCAGCAG CCTGAGCCTT CGCCATTCCT CAGAGCGCCT CAGTGACACC 
CCTGGATCCT TCCAGCCACC TTCCCTGGAA ATTCTGCTGT CTAGTTGCTC CTTGTGCCGC 
GCCTGTGATT CGCTCGTGTA CGATGAGGAG ATCATGGCTG GCTGGGCACC TGATGACTCC 
AACCTCAACA CAACCTGCCC CTTCTGCGCA TGCCCCTTTG TGCCCCTGCT CAGTGTCCAG 
ACCCTTGATT TCCGACCCAG TGCCCCCAGC CCCAAGCCTG CCCCTGCGGA TGCCGGAGGC 
AACAAAGACG CACCTGTCCC TGGGGGCCCA GGACCTGTGC TCAGTAACCG CAGGCTCTGC 
CTTGCCCTGG ATGAGCCCCA GCTCTGCAAT GGGCACATGG GGGGTGCCTC CCGGCGGGTC 
GAGGGTGGGG CATGGGCATA CCTGAGCCCC CTGGTGCTGC GTAAGGAGTT GGAGTCGCTG 
ATAGAGAACG AGGGCAGCGA GGTGCTGGCA TTGCCTGAGC TGCCTGCTAC CCACCCCATT 
ATCTTCTGGA ACCTTCTGTG GTATTTCCAG CGACTGCAAC TGCCCAGTAT TCTGCCATGC 
CTGGTGCTGG CCTCCTGTGA TGGGCCCCCA CCCCCCCAGG CCCCATCTCC TTGGCTAACA 
CCTGATCCAG CCTCTGTGCA GGTGCGGCTG CTGTGGGATG TCCTGACCCC CGACCCCAAT 
AGCTGCCCAC CTCTGTATGT GCTCTGGAGA GTCCACAGCC AGATCCCCCA GCGGGTAGTA 
TGGCCAGGCC CAGTACCTGC CTCCCTTAGC CTGGCGTTGC TGGAGTCGGT GCTGCGCCAT 
GTTGGTCTCA ATGAGGTGCA CAAGGCTGTA GGGCTCCTGC TGGAAACTCT AGGGCCTCCT 
CCCACAGGCC TGCACCTACA GAGGGGCATC TACCGTGAGA TCTTATTCCT GACAATGGCT 
GCTATGGGCA AGGACCACAT GGACATAGTG GCCTTCGACA AGAAGTACAA GTCTGCCTTT 
AACAAGCTGG CCAGCAGCAT GGGCAAGGAG GAGCTGAGGC AGCGGCGGGC ACAGATGCCC 
ACTCCAAAGG CCATTGATTG CCGAAAATGT TTTGGAGCAC CTCTGGAATG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_001493858.5 ) cDNA ORF clone, Equus caballus(horse) -> XP_001493908.3 Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb20931
Clone ID Related Accession (Same CDS sequence)	XM_001493858.5 , XM_001493858.6
Accession Version	XM_001493858.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867419.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001493858.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGCCCCATC TCCTTGGCTA ACACCTGATC CAGCCTCTGT GCAGGTGCGG 
CTGCTGTGGG ATGTCCTGAC CCCCGACCCC AATAGCTGCC CACCTCTGTA TGTGCTCTGG 
AGAGTCCACA GCCAGATCCC CCAGCGGGTA GTATGGCCAG GCCCAGTACC TGCCTCCCTT 
AGCCTGGCGT TGCTGGAGTC GGTGCTGCGC CATGTTGGTC TCAATGAGGT GCACAAGGCT 
GTAGGGCTCC TGCTGGAAAC TCTAGGGCCT CCTCCCACAG GCCTGCACCT ACAGAGGGGC 
ATCTACCGTG AGATCTTATT CCTGACAATG GCTGCTATGG GCAAGGACCA CATGGACATA 
GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC TTTAACAAGC TGGCCAGCAG CATGGGCAAG 
GAGGAGCTGA GGCAGCGGCG GGCACAGATG CCCACTCCAA AGGCCATTGA TTGCCGAAAA 
TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001493908.3
CDS	744..5270
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAMYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATVECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLLPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLRTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVPEGSEPTPQYCYDGFPELQAELFESPQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVQVMLEMLRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERRKQRQQQQQQQQQQKQQAAAQQKTGSSQTEPSLERPSPTRPLHRQTTWAGRSLRDPASPSGRLVKSGSLGSAQGAQPTVEAGVAHMIEALGVLKPRGSPVPWHDGSLSDLSLTGEEPAPGGSPGDSGSALSTQSSEALEGLSGRVPKASGHQDEASTPRRGLGARLQQLLTPSRRSPTSRIPPPELPPDLPPPARRSPMDSLLRPRERPGSTASESSASLGSEWDLSESSVSSLSLRHSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDFRPSAPSPKPAPADAGGNKDAPVPGGPGPVLSNRRLCLALDEPQLCNGHMGGASRRVEGGAWAYLSPLVLRKELESLIENEGSEVLALPELPATHPIIFWNLLWYFQRLQLPSILPCLVLASCDGPPPPQAPSPWLTPDPASVQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAAMGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_001493858.5
Organism	Equus caballus(horse)
Definition	Equus caballus DENN/MADD domain containing 4B (DENND4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001493858.5

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGCCCCATC TCCTTGGCTA ACACCTGATC CAGCCTCTGT GCAGGTGCGG 
CTGCTGTGGG ATGTCCTGAC CCCCGACCCC AATAGCTGCC CACCTCTGTA TGTGCTCTGG 
AGAGTCCACA GCCAGATCCC CCAGCGGGTA GTATGGCCAG GCCCAGTACC TGCCTCCCTT 
AGCCTGGCGT TGCTGGAGTC GGTGCTGCGC CATGTTGGTC TCAATGAGGT GCACAAGGCT 
GTAGGGCTCC TGCTGGAAAC TCTAGGGCCT CCTCCCACAG GCCTGCACCT ACAGAGGGGC 
ATCTACCGTG AGATCTTATT CCTGACAATG GCTGCTATGG GCAAGGACCA CATGGACATA 
GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC TTTAACAAGC TGGCCAGCAG CATGGGCAAG 
GAGGAGCTGA GGCAGCGGCG GGCACAGATG CCCACTCCAA AGGCCATTGA TTGCCGAAAA 
TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4B ( XM_001493858.6 ) cDNA ORF clone, Equus caballus(horse) -> XP_001493908.3 Equus caballus DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.

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CloneID	OHb20931
Clone ID Related Accession (Same CDS sequence)	XM_001493858.5 , XM_001493858.6
Accession Version	XM_001493858.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	DENN domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_001493858.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGCCCCATC TCCTTGGCTA ACACCTGATC CAGCCTCTGT GCAGGTGCGG 
CTGCTGTGGG ATGTCCTGAC CCCCGACCCC AATAGCTGCC CACCTCTGTA TGTGCTCTGG 
AGAGTCCACA GCCAGATCCC CCAGCGGGTA GTATGGCCAG GCCCAGTACC TGCCTCCCTT 
AGCCTGGCGT TGCTGGAGTC GGTGCTGCGC CATGTTGGTC TCAATGAGGT GCACAAGGCT 
GTAGGGCTCC TGCTGGAAAC TCTAGGGCCT CCTCCCACAG GCCTGCACCT ACAGAGGGGC 
ATCTACCGTG AGATCTTATT CCTGACAATG GCTGCTATGG GCAAGGACCA CATGGACATA 
GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC TTTAACAAGC TGGCCAGCAG CATGGGCAAG 
GAGGAGCTGA GGCAGCGGCG GGCACAGATG CCCACTCCAA AGGCCATTGA TTGCCGAAAA 
TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001493908.3
CDS	1039..5565
Translation	MEADAVSEGGAMAEERPPRLVDYFVIAGLAGNGAPIPEETWVPEPSGPLRPPRPAEPITDVAVIARALGEEVPQGYTCIQASAGGHPLELSAGLLGGTQPVICYRRGRDKPPLVELGVLYEGKERPKPGFQVLDTTPYSHSANLAPPGPGHPRTYLTYRRAAEGAGLHALGITDLCLVLPSKGEGTPHTYCRLPRNLNPGMWGPAMYLCYKVGLAKANTLVYEAELLGRYPEEDNEAFPLPESVPVFCLPMGATVECWPAQTKYPVPVFSTFVLTGAAGDKVYGAALQFYEAFPRARLSERQARALGLLSAVERGRALGGRAVRSRRAIAVLSRWPAFPAFRAFLTFLYRYSVSGPHRLPLEAHISHFIHNVPFPSPQRPRILVQMSPYDNLLLCQPVSSPLPLSGASFLQLLQSLGPELAITLLLAVLTEHKLLVHSLRPDLLTSVCEALVSMIFPLHWQCPYIPLCPLVLADVLSAPVPFIVGIHSSYFDLHDPPADVICVDLDTNTLFQTEEKKPLSPRTLPRRPYKVLLATLTNLYQQLDQTYTGPEEEASLEFLLTDYEAVCGRRARLEREVQGAFLRFMACLLKGYRDFLLPLTQAPSEGARDVDNLFFLQGFLKSRERSSHKLYSQLLRTQMFSQFIEECSFGSARHAALEFFDSCVDKVHPEQEKPEPTPLVELEELSGSELTVFITPPEEPPVPEGSEPTPQYCYDGFPELQAELFESPQEQPGALPVPGPSRSAPSSPAPRRTKQEMKVAQRMAQKSAAVPELWARCLLGHCYGLWFLCLPAYVRSAPSRVRALHTAYQVLRQMESRKVVLPDEVCYRVLMQLCSHYGQPVLSVQVMLEMLRAGIVPNTITYGYYNKAVLESKWPSGTPGGRLRWAKLRNVVLGAAQFRQPLRERRKQRQQQQQQQQQQKQQAAAQQKTGSSQTEPSLERPSPTRPLHRQTTWAGRSLRDPASPSGRLVKSGSLGSAQGAQPTVEAGVAHMIEALGVLKPRGSPVPWHDGSLSDLSLTGEEPAPGGSPGDSGSALSTQSSEALEGLSGRVPKASGHQDEASTPRRGLGARLQQLLTPSRRSPTSRIPPPELPPDLPPPARRSPMDSLLRPRERPGSTASESSASLGSEWDLSESSVSSLSLRHSSERLSDTPGSFQPPSLEILLSSCSLCRACDSLVYDEEIMAGWAPDDSNLNTTCPFCACPFVPLLSVQTLDFRPSAPSPKPAPADAGGNKDAPVPGGPGPVLSNRRLCLALDEPQLCNGHMGGASRRVEGGAWAYLSPLVLRKELESLIENEGSEVLALPELPATHPIIFWNLLWYFQRLQLPSILPCLVLASCDGPPPPQAPSPWLTPDPASVQVRLLWDVLTPDPNSCPPLYVLWRVHSQIPQRVVWPGPVPASLSLALLESVLRHVGLNEVHKAVGLLLETLGPPPTGLHLQRGIYREILFLTMAAMGKDHMDIVAFDKKYKSAFNKLASSMGKEELRQRRAQMPTPKAIDCRKCFGAPLEC

Target ORF information:

RefSeq Version	XM_001493858.6
Organism	Equus caballus(horse)
Definition	Equus caballus DENN domain containing 4B (DENND4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001493858.6

ORF Insert Sequence:

ATGGAGGCAG ATGCAGTGAG TGAGGGGGGG GCCATGGCGG AGGAGCGGCC CCCCCGGCTC 
GTGGATTACT TCGTGATAGC TGGGCTTGCA GGGAACGGAG CACCTATCCC TGAGGAAACG 
TGGGTCCCTG AACCCAGTGG GCCCCTGCGC CCTCCCCGGC CAGCTGAGCC CATCACAGAT 
GTGGCAGTCA TCGCTAGGGC ACTGGGCGAG GAGGTGCCCC AGGGCTACAC ATGCATCCAG 
GCTTCTGCTG GGGGCCACCC CTTGGAACTC AGTGCTGGGC TTCTGGGAGG AACTCAACCT 
GTCATCTGCT ACCGCAGGGG CCGTGACAAA CCCCCCCTCG TTGAGCTGGG GGTGTTGTAT 
GAGGGGAAGG AACGTCCCAA GCCTGGCTTC CAAGTGCTAG ACACGACACC CTACAGCCAC 
TCAGCCAACC TGGCCCCTCC AGGACCTGGG CACCCCCGCA CCTACCTCAC TTACCGGCGG 
GCAGCAGAAG GGGCAGGGCT GCATGCCCTG GGCATTACTG ACCTCTGCCT GGTGCTGCCC 
AGCAAGGGCG AGGGCACCCC TCACACGTAC TGCCGATTGC CCCGCAACCT CAACCCTGGC 
ATGTGGGGCC CAGCGATGTA CCTGTGCTAC AAGGTGGGCC TGGCCAAGGC CAACACTCTG 
GTGTATGAGG CAGAACTGCT GGGCCGCTAC CCAGAGGAGG ACAATGAGGC GTTCCCGCTG 
CCCGAGTCCG TGCCGGTCTT CTGCCTGCCC ATGGGGGCCA CTGTCGAGTG CTGGCCCGCC 
CAGACCAAGT ACCCCGTACC CGTCTTCTCC ACCTTTGTGC TCACGGGCGC AGCTGGTGAT 
AAGGTGTACG GCGCCGCCCT GCAGTTCTAC GAGGCGTTCC CGCGGGCCAG GCTGTCGGAG 
CGGCAGGCGC GGGCGCTGGG CCTGCTGAGC GCGGTGGAGC GCGGCCGCGC GCTGGGGGGC 
CGGGCCGTGC GCAGCCGCCG CGCCATCGCC GTGCTGTCCC GCTGGCCTGC CTTCCCTGCC 
TTCCGCGCTT TCCTCACCTT CCTCTACCGC TACTCCGTCT CAGGCCCCCA CCGCCTACCC 
TTGGAAGCGC ACATCTCCCA CTTCATTCAC AACGTCCCCT TCCCTTCCCC ACAGAGACCC 
CGCATCCTGG TGCAGATGTC CCCGTATGAC AACCTGCTCC TCTGCCAGCC TGTGTCCTCA 
CCCTTGCCCC TCAGTGGTGC CAGCTTCCTG CAGCTGCTGC AGAGCCTGGG CCCTGAGCTG 
GCTATCACGC TGCTGCTGGC TGTGCTCACA GAGCACAAAC TGCTAGTCCA CTCGCTGCGG 
CCGGACCTGC TCACCAGTGT CTGCGAGGCC CTCGTCTCTA TGATCTTCCC GCTGCACTGG 
CAGTGCCCCT ACATTCCACT GTGCCCACTG GTGCTGGCAG ATGTGCTGAG CGCCCCCGTG 
CCCTTCATTG TGGGTATCCA CTCCAGCTAC TTCGATCTGC ATGACCCTCC TGCTGATGTC 
ATCTGTGTTG ATCTTGATAC CAACACACTC TTCCAGACTG AGGAAAAGAA GCCCCTCTCC 
CCTCGGACTC TGCCCCGCAG ACCCTACAAA GTTCTGTTGG CTACACTGAC AAACCTATAT 
CAGCAGCTGG ATCAGACGTA TACTGGACCC GAGGAGGAGG CATCCCTGGA ATTCCTGCTG 
ACAGACTACG AGGCAGTGTG CGGCCGCCGG GCCCGGCTGG AGCGTGAGGT CCAGGGAGCC 
TTCCTCCGCT TCATGGCCTG TCTGCTCAAG GGCTACCGGG ACTTCCTGCT CCCGCTCACC 
CAGGCCCCCT CGGAGGGGGC TCGTGATGTT GACAACCTGT TCTTCCTGCA GGGCTTCCTC 
AAGTCCCGGG AACGCTCCAG CCATAAGCTG TACTCTCAGC TGCTACGCAC ACAGATGTTC 
TCGCAGTTCA TCGAGGAATG CTCTTTTGGC TCTGCTCGGC ATGCTGCCCT TGAATTCTTT 
GACTCTTGTG TTGACAAGGT CCACCCAGAG CAGGAGAAGC CTGAACCGAC CCCCCTAGTG 
GAGCTGGAGG AGCTGTCGGG AAGTGAGCTC ACTGTGTTTA TCACACCTCC TGAGGAGCCT 
CCAGTGCCAG AGGGCAGTGA ACCCACTCCC CAGTACTGCT ATGATGGGTT CCCAGAGCTA 
CAGGCTGAGC TGTTTGAGTC TCCTCAAGAG CAGCCTGGGG CTCTGCCTGT GCCGGGCCCC 
TCCCGTAGTG CCCCCAGCAG TCCTGCCCCA CGCCGTACCA AACAGGAGAT GAAGGTCGCA 
CAACGGATGG CGCAGAAGTC AGCAGCTGTG CCTGAGCTGT GGGCCCGGTG CCTTCTGGGG 
CACTGCTACG GGCTGTGGTT CCTGTGTCTG CCTGCTTACG TGCGCTCGGC GCCCTCCCGG 
GTGCGGGCAC TGCACACGGC CTACCAAGTG CTGCGCCAGA TGGAGAGCCG CAAGGTGGTG 
CTGCCCGATG AGGTGTGTTA CCGGGTGCTG ATGCAGCTCT GCTCACACTA TGGGCAGCCA 
GTGCTGTCTG TTCAGGTCAT GCTAGAGATG CTGCGAGCAG GCATTGTGCC CAACACCATC 
ACCTACGGCT ACTACAACAA GGCTGTGCTG GAAAGCAAGT GGCCGTCTGG TACACCGGGT 
GGGCGCCTGC GCTGGGCCAA GCTCCGGAAC GTCGTCCTGG GGGCTGCTCA GTTCCGCCAG 
CCCTTGAGAG AACGGCGGAA GCAGCGGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGAAG 
CAGCAGGCAG CAGCACAACA AAAGACAGGC AGCTCCCAGA CAGAGCCCTC TCTGGAGCGC 
CCCTCCCCAA CCCGCCCCCT TCATCGCCAG ACTACTTGGG CTGGGCGAAG CCTGCGGGAC 
CCAGCCTCAC CCTCAGGGCG CCTGGTGAAG AGTGGCAGCC TGGGCAGTGC CCAAGGGGCA 
CAGCCCACTG TGGAGGCTGG CGTAGCCCAC ATGATAGAGG CCTTGGGGGT ATTGAAACCC 
CGAGGATCAC CTGTGCCCTG GCACGATGGA AGCCTTTCAG ACTTGAGCCT GACCGGGGAG 
GAGCCAGCAC CTGGAGGAAG CCCAGGGGAC TCTGGCTCAG CCCTGAGTAC TCAGTCCAGT 
GAAGCCCTGG AAGGGCTAAG TGGCCGGGTA CCCAAGGCTA GTGGGCATCA GGATGAGGCC 
AGCACCCCCC GACGAGGGCT GGGTGCCCGC CTCCAACAGC TGCTCACTCC TTCCCGCCGC 
TCCCCGACCT CTCGCATTCC CCCACCTGAG CTGCCCCCTG ACCTGCCTCC CCCAGCCCGC 
CGCAGCCCCA TGGACAGCCT TCTGCGCCCC CGGGAGCGCC CTGGATCCAC TGCCTCTGAG 
AGCTCAGCCT CTCTGGGCAG TGAGTGGGAC CTCTCAGAAT CTTCCGTCAG CAGCCTGAGC 
CTTCGCCATT CCTCAGAGCG CCTCAGTGAC ACCCCTGGAT CCTTCCAGCC ACCTTCCCTG 
GAAATTCTGC TGTCTAGTTG CTCCTTGTGC CGCGCCTGTG ATTCGCTCGT GTACGATGAG 
GAGATCATGG CTGGCTGGGC ACCTGATGAC TCCAACCTCA ACACAACCTG CCCCTTCTGC 
GCATGCCCCT TTGTGCCCCT GCTCAGTGTC CAGACCCTTG ATTTCCGACC CAGTGCCCCC 
AGCCCCAAGC CTGCCCCTGC GGATGCCGGA GGCAACAAAG ACGCACCTGT CCCTGGGGGC 
CCAGGACCTG TGCTCAGTAA CCGCAGGCTC TGCCTTGCCC TGGATGAGCC CCAGCTCTGC 
AATGGGCACA TGGGGGGTGC CTCCCGGCGG GTCGAGGGTG GGGCATGGGC ATACCTGAGC 
CCCCTGGTGC TGCGTAAGGA GTTGGAGTCG CTGATAGAGA ACGAGGGCAG CGAGGTGCTG 
GCATTGCCTG AGCTGCCTGC TACCCACCCC ATTATCTTCT GGAACCTTCT GTGGTATTTC 
CAGCGACTGC AACTGCCCAG TATTCTGCCA TGCCTGGTGC TGGCCTCCTG TGATGGGCCC 
CCACCCCCCC AGGCCCCATC TCCTTGGCTA ACACCTGATC CAGCCTCTGT GCAGGTGCGG 
CTGCTGTGGG ATGTCCTGAC CCCCGACCCC AATAGCTGCC CACCTCTGTA TGTGCTCTGG 
AGAGTCCACA GCCAGATCCC CCAGCGGGTA GTATGGCCAG GCCCAGTACC TGCCTCCCTT 
AGCCTGGCGT TGCTGGAGTC GGTGCTGCGC CATGTTGGTC TCAATGAGGT GCACAAGGCT 
GTAGGGCTCC TGCTGGAAAC TCTAGGGCCT CCTCCCACAG GCCTGCACCT ACAGAGGGGC 
ATCTACCGTG AGATCTTATT CCTGACAATG GCTGCTATGG GCAAGGACCA CATGGACATA 
GTGGCCTTCG ACAAGAAGTA CAAGTCTGCC TTTAACAAGC TGGCCAGCAG CATGGGCAAG 
GAGGAGCTGA GGCAGCGGCG GGCACAGATG CCCACTCCAA AGGCCATTGA TTGCCGAAAA 
TGTTTTGGAG CACCTCTGGA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DENND4B cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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