TJP1 cDNA ORF clone, Equus caballus(horse)

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb10216 XM_005602842.2
Latest version!
Equus caballus tight junction protein 1 (TJP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OHb41617 XM_023651557.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHb41618 XM_023651558.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHb41619 XM_023651560.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHb41620 XM_023651563.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHb41621 XM_023651565.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHb41622 XM_023651567.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHb41623 XM_023651569.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHb41623 XM_023651568.1
Latest version!
Equus caballus tight junction protein 1 (TJP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb10216
    Clone ID Related Accession (Same CDS sequence) XM_005602842.2
    Accession Version XM_005602842.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product LOW QUALITY PROTEIN: tight junction protein ZO-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867387.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005602842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGGAAA CAGCTATATG GGAACAACAC ACAGTGACGC TTCACAGGGC TCCTGGATTT 
    GGATTTGGAA TCGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGAGAAACT
    TCAATAGTAA TTTCAGATGT GCTGAAAGGA GGACCCGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CCATGGTTAA TGGAGTTTCG ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAACTAA GGAAAAGTGG GAAGAATGCA AAAATTACTA TCCGAAGGAA AAAGAAAGTT
    CAGATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG AAAATGAAGA AGATAGTTAT
    GATGAGGAAG TACATGATCC AAGAAGTGGC CGTGGTGGTC TGATTAATAG AAGGAGTGAG
    AAGAGTTGGG TGAGGGATAG AAGTGCAAGT AGAGAGAGGA GTTTGTCTCC ACGATCTGAT
    AGGCGATCTG TGGCCTCCAG TCAACCTGCC AAACCCACCA AAGTCACATT GGTGAAATCC
    CGGAAGAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGACGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACCCTATT GAACGTCCCT
    GATCTTGCTG ACAGTATCCA CTCTGCTAAC GCCTCTGAGA GAGATGACAT TTCAGAAATT
    CAGTCACTGG CATCAGATCA TTCCGGTCGA TCACACGACA GGCCTCCTCG CCACAGCCGG
    TCACGATCTC CTGACCAACG GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAACAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAA GAAGAGAGAA TTTCTAAACC TGGGGCCATC
    TCAACTCCCG TAAAGCATGC AGATGATCAT ACACCTAAAA CAGTGGAAGA AGTTATAGTT
    GAAAGAAATG AGAAACAAGC CCCGACTCTT CCAGAACCAA AGCCTGTATA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCTGTCAGT CCATCTGATG GTGTCCTACC TAATTCAACT
    CATGAAGATG GGATCCTTCG GCCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GCCTGGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCCG CAGCCAAAGA AGGCTTAGAA GAGGGTGATC AAATTCTCAG GGTAAACAAT
    GTAGATTTCA CAAACATCAT AAGAGAAGAA GCTGTCCTTT TCCTGCTTGA CCTCCCTAAA
    GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG TATCGTAGAA
    TCAGATGTAG GAGATTCTTT CTATATTAGA ACTCATTTTG AATATGAAAA AGAATCTCCC
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTCG TGGATACATT GTACAATGGA
    AAACTAGGCT CCTGGTTGGC TATTCGAATT GGTAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTGGCCAGTG TACAATACAC ACTTCCAAAA
    ACAGCAGGAG GAGACCGAGC TGACTTCTGG AGATTCCGGG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGATTTGTCA GCTCAGCCAG TTCAAACAAA GTTTCCAGCA
    TATGAAAGAG TTGTTCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGGGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CAGCGAAGCT CTGGCATTAT TCGTCTTCAT
    ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA
    GTTGATCGTC TGAATTACGC CCAGTGGTAT CCAATTGTAG TCTTCCTTAA CCCTGATTCT
    AAGCAAGGTG TGNAAACTAT GAGAATGAGG TTATGTCCAG AATCTCGGAA AAGTGCCAGG
    AAATTATATG AGCGGTCCCA TAAACTTCGT AAAAATAATC ACCATCTTTT CACAACTACA
    ATTAACCTAA ATTCAATGAA TGATGGTTGG TATGGTGCAT TAAAAGAAGC AATTCAACAA
    CAACAGAACC AGTTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAT
    CAAACATATC CTTCTTATTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC
    CCTGGATTTA AAACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTATCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT
    AATGTCAGAC TGGAGGAGCT CACCCCAGCT CCTCCCACCT CTTACTCACC ACAAGCTGAT
    TCTTTAAGAA CACCAAGTTC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAAGCATCA
    CTGTCGTCGC ATGTAGATCC AACAAAGGTA TATAGAAAGG ATCCTTATCC TGAGGACATG
    ATGAGACAGA ATCATGTTTT GAAACAGCCG GCTGTTGGTC ACCCAGGGCA GAGGGCAGAC
    AAAGAGCCTA ACCTGAGCTA TGAAGCCCAG CCCCCATATG TGGAGAAACA AGCCAGCAGA
    GACCTTGAGC AGCCCACATA TAGATATGAC TCCTCAAGCT ATGCAGAACA GTTTTCTCGA
    AACTATGACC ATCGTCTACA CTATGAAGAT CGCATCCCTA CATATGAAGA GCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTCTT TTGATAATCA GCACCCTCGA
    GACCTTGACT CCAGACAGCA TCCTGAAGAG TCCTCAGAAC GAGGGTATTT CCCACGTTTT
    GAAGAGCCAA CTCCTCTGTC CTATGACAGC AGACCACGCT ATGACCAACC ACCTAGAACC
    TCCACCCTAC GACATGAAGA GCAAGCAGCT CCTGGATATG ACATACATAA CAGATACAGA
    CCAGAAGCAC AGCCCTATTC TTCAGCAGGC CATAAGGCCT CTGAGGCCAA GCAGTATTTT
    GACCAATACC CACGAAGTTA TGAGCAAGTA CCATCACAAG GACTTACCTC TAAAGCAGGC
    CATTATGAGC CTCTCCATGG TGCTGCAGTT GTCACTCCTC TAATACCTGC ATCTCAGCAG
    AAGCCGGAAG TTCTGCCTTC AAGTACGAAG CCACTGCCTC CACCCCCAAC TCTCACTGAA
    GAAGAGGAAG ATCCAGCAAT GAAACCACAG TCTGTGCTCA CTAGAGTTAA AATGTTTGAA
    AACAAAAGAT CTGCATCTTT GGAGAACAAG AAGGATGAAA ACCACACCAC TAGTTTTAAG
    CCTCCAGAGG TAGCATCTAA ACCTCCAGTT GCTCCCATCA TTGGTCCTAA AGCCACTCCT
    CAAAATCAGT TCAGTGAACA TGACAAAACA CTCTACAGGG TCCCAGAACC TCAAAAACCT
    CAACTGAAGC CACCTGAAGA TATTGTCCGG TCAAATCATT ATGATCCTGA GGAAGATGAA
    GAATATTATC GAAAGCAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA CAAGCCTTCT
    CCACACATTC CTGCTAGCCA TCTCTCAGAG CCTGCCAAGC CAGTTCCTTC TCAGAACCAA
    ACAAATTTTT CTGGTTATTC TTTGAAGGGA AAGTCTACTG AAGCTGATGC TTTGGAGAGA
    TCATTTGGTG AGAAACGCTA TGACCCAATC CAGGCCACTC CCCCCCCTCC TCCATTGCCC
    TCCCAGTACA CCCAGCCTTC TCAGCCCGGC TCCAGCTCTT CTCTCGCCCT CCACACACAT
    GCCAAGGGAG GACATGGTGA AGGTAATTCA GCATCACTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGTCTGC TTTCTATCCC CAGAAAAGTT TCCAAGATAA AGCTCCAATT
    AATGGAGCTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACGCCAAAA
    CCATATACCA GTTCTGCTCG GCCGTTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAGGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAACC CCCTACTTCT
    CCAAAAACTC TGGTGAAAGC ACACAGTTCA GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CCATCCATGC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT ATGGAAGAGG ATGAAGATGA GGATGGTCAT
    ACCGTGGTGG CCACAGCCCG AGGCGTATTT AACAGCAATG GTGGGGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG AGACAACAGC ATCCTCCCGC CTTTAGATAA GGAGAAAGGT
    GAAACACTGC TGAGCCCCTT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCG
    GTGGAGCTAC GCCTGCCACA CTGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCAAATT ATCTTGTTGG AGCAAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005602899.1
    CDS37..5253
    Translation

    Target ORF information:

    RefSeq Version XM_005602842.2
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005602842.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGGAAA CAGCTATATG GGAACAACAC ACAGTGACGC TTCACAGGGC TCCTGGATTT 
    GGATTTGGAA TCGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGAGAAACT
    TCAATAGTAA TTTCAGATGT GCTGAAAGGA GGACCCGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CCATGGTTAA TGGAGTTTCG ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAACTAA GGAAAAGTGG GAAGAATGCA AAAATTACTA TCCGAAGGAA AAAGAAAGTT
    CAGATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG AAAATGAAGA AGATAGTTAT
    GATGAGGAAG TACATGATCC AAGAAGTGGC CGTGGTGGTC TGATTAATAG AAGGAGTGAG
    AAGAGTTGGG TGAGGGATAG AAGTGCAAGT AGAGAGAGGA GTTTGTCTCC ACGATCTGAT
    AGGCGATCTG TGGCCTCCAG TCAACCTGCC AAACCCACCA AAGTCACATT GGTGAAATCC
    CGGAAGAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGACGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACCCTATT GAACGTCCCT
    GATCTTGCTG ACAGTATCCA CTCTGCTAAC GCCTCTGAGA GAGATGACAT TTCAGAAATT
    CAGTCACTGG CATCAGATCA TTCCGGTCGA TCACACGACA GGCCTCCTCG CCACAGCCGG
    TCACGATCTC CTGACCAACG GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAACAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAA GAAGAGAGAA TTTCTAAACC TGGGGCCATC
    TCAACTCCCG TAAAGCATGC AGATGATCAT ACACCTAAAA CAGTGGAAGA AGTTATAGTT
    GAAAGAAATG AGAAACAAGC CCCGACTCTT CCAGAACCAA AGCCTGTATA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCTGTCAGT CCATCTGATG GTGTCCTACC TAATTCAACT
    CATGAAGATG GGATCCTTCG GCCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GCCTGGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCCG CAGCCAAAGA AGGCTTAGAA GAGGGTGATC AAATTCTCAG GGTAAACAAT
    GTAGATTTCA CAAACATCAT AAGAGAAGAA GCTGTCCTTT TCCTGCTTGA CCTCCCTAAA
    GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG TATCGTAGAA
    TCAGATGTAG GAGATTCTTT CTATATTAGA ACTCATTTTG AATATGAAAA AGAATCTCCC
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTCG TGGATACATT GTACAATGGA
    AAACTAGGCT CCTGGTTGGC TATTCGAATT GGTAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTGGCCAGTG TACAATACAC ACTTCCAAAA
    ACAGCAGGAG GAGACCGAGC TGACTTCTGG AGATTCCGGG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGATTTGTCA GCTCAGCCAG TTCAAACAAA GTTTCCAGCA
    TATGAAAGAG TTGTTCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGGGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CAGCGAAGCT CTGGCATTAT TCGTCTTCAT
    ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA
    GTTGATCGTC TGAATTACGC CCAGTGGTAT CCAATTGTAG TCTTCCTTAA CCCTGATTCT
    AAGCAAGGTG TGNAAACTAT GAGAATGAGG TTATGTCCAG AATCTCGGAA AAGTGCCAGG
    AAATTATATG AGCGGTCCCA TAAACTTCGT AAAAATAATC ACCATCTTTT CACAACTACA
    ATTAACCTAA ATTCAATGAA TGATGGTTGG TATGGTGCAT TAAAAGAAGC AATTCAACAA
    CAACAGAACC AGTTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAT
    CAAACATATC CTTCTTATTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC
    CCTGGATTTA AAACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTATCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT
    AATGTCAGAC TGGAGGAGCT CACCCCAGCT CCTCCCACCT CTTACTCACC ACAAGCTGAT
    TCTTTAAGAA CACCAAGTTC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAAGCATCA
    CTGTCGTCGC ATGTAGATCC AACAAAGGTA TATAGAAAGG ATCCTTATCC TGAGGACATG
    ATGAGACAGA ATCATGTTTT GAAACAGCCG GCTGTTGGTC ACCCAGGGCA GAGGGCAGAC
    AAAGAGCCTA ACCTGAGCTA TGAAGCCCAG CCCCCATATG TGGAGAAACA AGCCAGCAGA
    GACCTTGAGC AGCCCACATA TAGATATGAC TCCTCAAGCT ATGCAGAACA GTTTTCTCGA
    AACTATGACC ATCGTCTACA CTATGAAGAT CGCATCCCTA CATATGAAGA GCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTCTT TTGATAATCA GCACCCTCGA
    GACCTTGACT CCAGACAGCA TCCTGAAGAG TCCTCAGAAC GAGGGTATTT CCCACGTTTT
    GAAGAGCCAA CTCCTCTGTC CTATGACAGC AGACCACGCT ATGACCAACC ACCTAGAACC
    TCCACCCTAC GACATGAAGA GCAAGCAGCT CCTGGATATG ACATACATAA CAGATACAGA
    CCAGAAGCAC AGCCCTATTC TTCAGCAGGC CATAAGGCCT CTGAGGCCAA GCAGTATTTT
    GACCAATACC CACGAAGTTA TGAGCAAGTA CCATCACAAG GACTTACCTC TAAAGCAGGC
    CATTATGAGC CTCTCCATGG TGCTGCAGTT GTCACTCCTC TAATACCTGC ATCTCAGCAG
    AAGCCGGAAG TTCTGCCTTC AAGTACGAAG CCACTGCCTC CACCCCCAAC TCTCACTGAA
    GAAGAGGAAG ATCCAGCAAT GAAACCACAG TCTGTGCTCA CTAGAGTTAA AATGTTTGAA
    AACAAAAGAT CTGCATCTTT GGAGAACAAG AAGGATGAAA ACCACACCAC TAGTTTTAAG
    CCTCCAGAGG TAGCATCTAA ACCTCCAGTT GCTCCCATCA TTGGTCCTAA AGCCACTCCT
    CAAAATCAGT TCAGTGAACA TGACAAAACA CTCTACAGGG TCCCAGAACC TCAAAAACCT
    CAACTGAAGC CACCTGAAGA TATTGTCCGG TCAAATCATT ATGATCCTGA GGAAGATGAA
    GAATATTATC GAAAGCAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA CAAGCCTTCT
    CCACACATTC CTGCTAGCCA TCTCTCAGAG CCTGCCAAGC CAGTTCCTTC TCAGAACCAA
    ACAAATTTTT CTGGTTATTC TTTGAAGGGA AAGTCTACTG AAGCTGATGC TTTGGAGAGA
    TCATTTGGTG AGAAACGCTA TGACCCAATC CAGGCCACTC CCCCCCCTCC TCCATTGCCC
    TCCCAGTACA CCCAGCCTTC TCAGCCCGGC TCCAGCTCTT CTCTCGCCCT CCACACACAT
    GCCAAGGGAG GACATGGTGA AGGTAATTCA GCATCACTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGTCTGC TTTCTATCCC CAGAAAAGTT TCCAAGATAA AGCTCCAATT
    AATGGAGCTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACGCCAAAA
    CCATATACCA GTTCTGCTCG GCCGTTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAGGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAACC CCCTACTTCT
    CCAAAAACTC TGGTGAAAGC ACACAGTTCA GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CCATCCATGC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT ATGGAAGAGG ATGAAGATGA GGATGGTCAT
    ACCGTGGTGG CCACAGCCCG AGGCGTATTT AACAGCAATG GTGGGGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG AGACAACAGC ATCCTCCCGC CTTTAGATAA GGAGAAAGGT
    GAAACACTGC TGAGCCCCTT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCG
    GTGGAGCTAC GCCTGCCACA CTGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCAAATT ATCTTGTTGG AGCAAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41617
    Clone ID Related Accession (Same CDS sequence) XM_023651557.1
    Accession Version XM_023651557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ATCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCTCACCC CAGCTCCTCC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTTCTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAG CATCACTGTC GTCGCATGTA GATCCAACAA AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507325.1
    CDS23..5614
    Translation

    Target ORF information:

    RefSeq Version XM_023651557.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ATCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCTCACCC CAGCTCCTCC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTTCTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAG CATCACTGTC GTCGCATGTA GATCCAACAA AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41618
    Clone ID Related Accession (Same CDS sequence) XM_023651558.1
    Accession Version XM_023651558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ATCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCTCACCC CAGCTCCTCC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTTCTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAG CATCACTGTC GTCGCATGTA GATCCAACAA AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG CGTCCATGAC TCCTGACGGT
    GATCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CAAATTATCT TGTTGGAGCA
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507326.1
    CDS23..5575
    Translation

    Target ORF information:

    RefSeq Version XM_023651558.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ATCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCTCACCC CAGCTCCTCC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTTCTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAG CATCACTGTC GTCGCATGTA GATCCAACAA AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG CGTCCATGAC TCCTGACGGT
    GATCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CAAATTATCT TGTTGGAGCA
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41619
    Clone ID Related Accession (Same CDS sequence) XM_023651560.1
    Accession Version XM_023651560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ATCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCTCACCC CAGCTCCTCC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTTCTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAG CATCACTGTC GTCGCATGTA GATCCAACAA AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507328.1
    CDS1..5532
    Translation

    Target ORF information:

    RefSeq Version XM_023651560.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGAAAGCAGA AGCCTCATCT
    CCAGTCCCTT ATCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCTCACCC CAGCTCCTCC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTTCTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAG CATCACTGTC GTCGCATGTA GATCCAACAA AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41620
    Clone ID Related Accession (Same CDS sequence) XM_023651563.1
    Accession Version XM_023651563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507331.1
    CDS23..5374
    Translation

    Target ORF information:

    RefSeq Version XM_023651563.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41621
    Clone ID Related Accession (Same CDS sequence) XM_023651565.1
    Accession Version XM_023651565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCCGCGGC CACCAAGAAC ACAGTAATGG AGGAAACAGC TATATGGGAA 
    CAACACACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATCGC AATATCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGA GAAACTTCAA TAGTAATTTC AGATGTGCTG
    AAAGGAGGAC CCGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCCAT GGTTAATGGA
    GTTTCGATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAG
    AATGCAAAAA TTACTATCCG AAGGAAAAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGAAAA TGAAGAAGAT AGTTATGATG AGGAAGTACA TGATCCAAGA
    AGTGGCCGTG GTGGTCTGAT TAATAGAAGG AGTGAGAAGA GTTGGGTGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGTTT GTCTCCACGA TCTGATAGGC GATCTGTGGC CTCCAGTCAA
    CCTGCCAAAC CCACCAAAGT CACATTGGTG AAATCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAC
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT
    CAGAGAGATG AACGGGCTAC CCTATTGAAC GTCCCTGATC TTGCTGACAG TATCCACTCT
    GCTAACGCCT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCC
    GGTCGATCAC ACGACAGGCC TCCTCGCCAC AGCCGGTCAC GATCTCCTGA CCAACGGTCA
    GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAACAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAAGAAG AGAGAATTTC TAAACCTGGG GCCATCTCAA CTCCCGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCCCCG
    ACTCTTCCAG AACCAAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT CCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGCCT GGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCCGCAGC CAAAGAAGGC
    TTAGAAGAGG GTGATCAAAT TCTCAGGGTA AACAATGTAG ATTTCACAAA CATCATAAGA
    GAAGAAGCTG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGTATC GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACTC ATTTTGAATA TGAAAAAGAA TCTCCCTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA TACATTGTAC AATGGAAAAC TAGGCTCCTG GTTGGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGTTGG CCAGTGTACA ATACACACTT CCAAAAACAG CAGGAGGAGA CCGAGCTGAC
    TTCTGGAGAT TCCGGGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCAGCTC AGCCAGTTCA AACAAAGTTT CCAGCATATG AAAGAGTTGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGA
    ACTGACCAGC GAAGCTCTGG CATTATTCGT CTTCATACAA TAAAGCAAAT AATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTACGCCCAG
    TGGTATCCAA TTGTAGTCTT CCTTAACCCT GATTCTAAGC AAGGTGTGAA AACTATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAGCG GTCCCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTCACA ACTACAATTA ACCTAAATTC AATGAATGAT
    GGTTGGTATG GTGCATTAAA AGAAGCAATT CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAATCAAA CATATCCTTC TTATTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TATCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCTCACC
    CCAGCTCCTC CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTTCTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA GCATCACTGT CGTCGCATGT AGATCCAACA
    AAGGTATATA GAAAGGATCC TTATCCTGAG GACATGATGA GACAGAATCA TGTTTTGAAA
    CAGCCGGCTG TTGGTCACCC AGGGCAGAGG GCAGACAAAG AGCCTAACCT GAGCTATGAA
    GCCCAGCCCC CATATGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACATATAGA
    TATGACTCCT CAAGCTATGC AGAACAGTTT TCTCGAAACT ATGACCATCG TCTACACTAT
    GAAGATCGCA TCCCTACATA TGAAGAGCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTCTTTTGA TAATCAGCAC CCTCGAGACC TTGACTCCAG ACAGCATCCT
    GAAGAGTCCT CAGAACGAGG GTATTTCCCA CGTTTTGAAG AGCCAACTCC TCTGTCCTAT
    GACAGCAGAC CACGCTATGA CCAACCACCT AGAACCTCCA CCCTACGACA TGAAGAGCAA
    GCAGCTCCTG GATATGACAT ACATAACAGA TACAGACCAG AAGCACAGCC CTATTCTTCA
    GCAGGCCATA AGGCCTCTGA GGCCAAGCAG TATTTTGACC AATACCCACG AAGTTATGAG
    CAAGTACCAT CACAAGGACT TACCTCTAAA GCAGGCCATT ATGAGCCTCT CCATGGTGCT
    GCAGTTGTCA CTCCTCTAAT ACCTGCATCT CAGCAGAAGC CGGAAGTTCT GCCTTCAAGT
    ACGAAGCCAC TGCCTCCACC CCCAACTCTC ACTGAAGAAG AGGAAGATCC AGCAATGAAA
    CCACAGTCTG TGCTCACTAG AGTTAAAATG TTTGAAAACA AAAGATCTGC ATCTTTGGAG
    AACAAGAAGG ATGAAAACCA CACCACTAGT TTTAAGCCTC CAGAGGTAGC ATCTAAACCT
    CCAGTTGCTC CCATCATTGG TCCTAAAGCC ACTCCTCAAA ATCAGTTCAG TGAACATGAC
    AAAACACTCT ACAGGGTCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTCCGGTCAA ATCATTATGA TCCTGAGGAA GATGAAGAAT ATTATCGAAA GCAGCTCTCA
    TACTTTGACC GAAGAAGTTT TGAGAACAAG CCTTCTCCAC ACATTCCTGC TAGCCATCTC
    TCAGAGCCTG CCAAGCCAGT TCCTTCTCAG AACCAAACAA ATTTTTCTGG TTATTCTTTG
    AAGGGAAAGT CTACTGAAGC TGATGCTTTG GAGAGATCAT TTGGTGAGAA ACGCTATGAC
    CCAATCCAGG CCACTCCCCC CCCTCCTCCA TTGCCCTCCC AGTACACCCA GCCTTCTCAG
    CCCGGCTCCA GCTCTTCTCT CGCCCTCCAC ACACATGCCA AGGGAGGACA TGGTGAAGGT
    AATTCAGCAT CACTGGATTT TCAGAATTCC TTAGTGTCCA AACCAGACCC ACCTCCATCT
    CAGAATAAGC CAGCAACTTT CAGACCACCA AACCGAGAAG ATACTGCTCA GTCTGCTTTC
    TATCCCCAGA AAAGTTTCCA AGATAAAGCT CCAATTAATG GAGCTGAACA GACTCAGAAA
    ACAGTCACTC CAGCATACAA TCGATTCACG CCAAAACCAT ATACCAGTTC TGCTCGGCCG
    TTTGAACGCA AGTTTGAAAG TCCTAAATTC AATCACAATC TTCTGCCAGG TGAAACTGCA
    CATAAACCTG ACTTGTCTTC AAAACCCCCT ACTTCTCCAA AAACTCTGGT GAAAGCACAC
    AGTTCAGCAC AGCCTCCTGA GTTTGACAGT GGAGTTGAAA CTTTCTCCAT CCATGCAGAT
    AAGCCTAAAT ATCAAATAAA TAATATCAGC ACAGTGCCTA AAGCTATTCC TGTGAGTCCT
    TCAGCTATGG AAGAGGATGA AGATGAGGAT GGTCATACCG TGGTGGCCAC AGCCCGAGGC
    GTATTTAACA GCAATGGTGG GGTGTTGAGT TCCATAGAAA CTGGTGTTAG TATAATTATC
    CCACAAGGAG CCATTCCTGA AGGAATTGAG CAGGAAATCT ATTTCAAGGT CTGCCGAGAC
    AACAGCATCC TCCCGCCTTT AGATAAGGAG AAAGGTGAAA CACTGCTGAG CCCCTTAGTG
    ATGTGTGGGC CCCATGGCCT CAAGTTCCTG AAGCCGGTGG AGCTACGCCT GCCACACTGT
    GCGTCCATGA CTCCTGACGG TTGGTCTTTT GCTCTAAAAT CATCCGACTC CTCGTCGGGT
    GATCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CAAATTATCT TGTTGGAGCA
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507333.1
    CDS101..5413
    Translation

    Target ORF information:

    RefSeq Version XM_023651565.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCCGCGGC CACCAAGAAC ACAGTAATGG AGGAAACAGC TATATGGGAA 
    CAACACACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATCGC AATATCTGGT
    GGAAGAGATA ATCCTCATTT TCAGAGTGGA GAAACTTCAA TAGTAATTTC AGATGTGCTG
    AAAGGAGGAC CCGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCCAT GGTTAATGGA
    GTTTCGATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAG
    AATGCAAAAA TTACTATCCG AAGGAAAAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGAAAA TGAAGAAGAT AGTTATGATG AGGAAGTACA TGATCCAAGA
    AGTGGCCGTG GTGGTCTGAT TAATAGAAGG AGTGAGAAGA GTTGGGTGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGTTT GTCTCCACGA TCTGATAGGC GATCTGTGGC CTCCAGTCAA
    CCTGCCAAAC CCACCAAAGT CACATTGGTG AAATCCCGGA AGAATGAAGA ATATGGTCTT
    CGATTGGCCA GCCATATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAC
    GGCAATATTC AAGAAGGCGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAGTTAAA AATGGTAGTT
    CAGAGAGATG AACGGGCTAC CCTATTGAAC GTCCCTGATC TTGCTGACAG TATCCACTCT
    GCTAACGCCT CTGAGAGAGA TGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCC
    GGTCGATCAC ACGACAGGCC TCCTCGCCAC AGCCGGTCAC GATCTCCTGA CCAACGGTCA
    GAGCCTTCTG ATCATTCCAG ACACTCTCCA CAACAGCCCA GCAATGGCAG TCTCCGGAGC
    AGAGAAGAAG AGAGAATTTC TAAACCTGGG GCCATCTCAA CTCCCGTAAA GCATGCAGAT
    GATCATACAC CTAAAACAGT GGAAGAAGTT ATAGTTGAAA GAAATGAGAA ACAAGCCCCG
    ACTCTTCCAG AACCAAAGCC TGTATATGCT CAAGTTGGAC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT CCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGCCT GGCTGGTGGA
    AATGATGTCG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCCGCAGC CAAAGAAGGC
    TTAGAAGAGG GTGATCAAAT TCTCAGGGTA AACAATGTAG ATTTCACAAA CATCATAAGA
    GAAGAAGCTG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGTATC GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACTC ATTTTGAATA TGAAAAAGAA TCTCCCTACG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTCGTGGA TACATTGTAC AATGGAAAAC TAGGCTCCTG GTTGGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGTTGG CCAGTGTACA ATACACACTT CCAAAAACAG CAGGAGGAGA CCGAGCTGAC
    TTCTGGAGAT TCCGGGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCAGCTC AGCCAGTTCA AACAAAGTTT CCAGCATATG AAAGAGTTGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATCTTT GGACCAATAG CTGATGTTGC CAGGGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAG ATTGCAAAAA GTGAACCACG AGATGCTGGA
    ACTGACCAGC GAAGCTCTGG CATTATTCGT CTTCATACAA TAAAGCAAAT AATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTGAA TTACGCCCAG
    TGGTATCCAA TTGTAGTCTT CCTTAACCCT GATTCTAAGC AAGGTGTGAA AACTATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAAT TATATGAGCG GTCCCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTCACA ACTACAATTA ACCTAAATTC AATGAATGAT
    GGTTGGTATG GTGCATTAAA AGAAGCAATT CAACAACAAC AGAACCAGTT GGTATGGGTT
    TCTGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCCCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA TACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAATCAAA CATATCCTTC TTATTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAAAC AGCCTCTCAA
    CAGAAAGCAG AAGCCTCATC TCCAGTCCCT TATCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCTCACC
    CCAGCTCCTC CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTTCTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA GCATCACTGT CGTCGCATGT AGATCCAACA
    AAGGTATATA GAAAGGATCC TTATCCTGAG GACATGATGA GACAGAATCA TGTTTTGAAA
    CAGCCGGCTG TTGGTCACCC AGGGCAGAGG GCAGACAAAG AGCCTAACCT GAGCTATGAA
    GCCCAGCCCC CATATGTGGA GAAACAAGCC AGCAGAGACC TTGAGCAGCC CACATATAGA
    TATGACTCCT CAAGCTATGC AGAACAGTTT TCTCGAAACT ATGACCATCG TCTACACTAT
    GAAGATCGCA TCCCTACATA TGAAGAGCAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CAACCTCGGC CTTCTTTTGA TAATCAGCAC CCTCGAGACC TTGACTCCAG ACAGCATCCT
    GAAGAGTCCT CAGAACGAGG GTATTTCCCA CGTTTTGAAG AGCCAACTCC TCTGTCCTAT
    GACAGCAGAC CACGCTATGA CCAACCACCT AGAACCTCCA CCCTACGACA TGAAGAGCAA
    GCAGCTCCTG GATATGACAT ACATAACAGA TACAGACCAG AAGCACAGCC CTATTCTTCA
    GCAGGCCATA AGGCCTCTGA GGCCAAGCAG TATTTTGACC AATACCCACG AAGTTATGAG
    CAAGTACCAT CACAAGGACT TACCTCTAAA GCAGGCCATT ATGAGCCTCT CCATGGTGCT
    GCAGTTGTCA CTCCTCTAAT ACCTGCATCT CAGCAGAAGC CGGAAGTTCT GCCTTCAAGT
    ACGAAGCCAC TGCCTCCACC CCCAACTCTC ACTGAAGAAG AGGAAGATCC AGCAATGAAA
    CCACAGTCTG TGCTCACTAG AGTTAAAATG TTTGAAAACA AAAGATCTGC ATCTTTGGAG
    AACAAGAAGG ATGAAAACCA CACCACTAGT TTTAAGCCTC CAGAGGTAGC ATCTAAACCT
    CCAGTTGCTC CCATCATTGG TCCTAAAGCC ACTCCTCAAA ATCAGTTCAG TGAACATGAC
    AAAACACTCT ACAGGGTCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTCCGGTCAA ATCATTATGA TCCTGAGGAA GATGAAGAAT ATTATCGAAA GCAGCTCTCA
    TACTTTGACC GAAGAAGTTT TGAGAACAAG CCTTCTCCAC ACATTCCTGC TAGCCATCTC
    TCAGAGCCTG CCAAGCCAGT TCCTTCTCAG AACCAAACAA ATTTTTCTGG TTATTCTTTG
    AAGGGAAAGT CTACTGAAGC TGATGCTTTG GAGAGATCAT TTGGTGAGAA ACGCTATGAC
    CCAATCCAGG CCACTCCCCC CCCTCCTCCA TTGCCCTCCC AGTACACCCA GCCTTCTCAG
    CCCGGCTCCA GCTCTTCTCT CGCCCTCCAC ACACATGCCA AGGGAGGACA TGGTGAAGGT
    AATTCAGCAT CACTGGATTT TCAGAATTCC TTAGTGTCCA AACCAGACCC ACCTCCATCT
    CAGAATAAGC CAGCAACTTT CAGACCACCA AACCGAGAAG ATACTGCTCA GTCTGCTTTC
    TATCCCCAGA AAAGTTTCCA AGATAAAGCT CCAATTAATG GAGCTGAACA GACTCAGAAA
    ACAGTCACTC CAGCATACAA TCGATTCACG CCAAAACCAT ATACCAGTTC TGCTCGGCCG
    TTTGAACGCA AGTTTGAAAG TCCTAAATTC AATCACAATC TTCTGCCAGG TGAAACTGCA
    CATAAACCTG ACTTGTCTTC AAAACCCCCT ACTTCTCCAA AAACTCTGGT GAAAGCACAC
    AGTTCAGCAC AGCCTCCTGA GTTTGACAGT GGAGTTGAAA CTTTCTCCAT CCATGCAGAT
    AAGCCTAAAT ATCAAATAAA TAATATCAGC ACAGTGCCTA AAGCTATTCC TGTGAGTCCT
    TCAGCTATGG AAGAGGATGA AGATGAGGAT GGTCATACCG TGGTGGCCAC AGCCCGAGGC
    GTATTTAACA GCAATGGTGG GGTGTTGAGT TCCATAGAAA CTGGTGTTAG TATAATTATC
    CCACAAGGAG CCATTCCTGA AGGAATTGAG CAGGAAATCT ATTTCAAGGT CTGCCGAGAC
    AACAGCATCC TCCCGCCTTT AGATAAGGAG AAAGGTGAAA CACTGCTGAG CCCCTTAGTG
    ATGTGTGGGC CCCATGGCCT CAAGTTCCTG AAGCCGGTGG AGCTACGCCT GCCACACTGT
    GCGTCCATGA CTCCTGACGG TTGGTCTTTT GCTCTAAAAT CATCCGACTC CTCGTCGGGT
    GATCCTAAAA CCTGGCAAAA CAAGTGTCTT CCTGGAGATC CAAATTATCT TGTTGGAGCA
    AACTGTGTTT CTGTCCTGAT TGACCACTTT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41622
    Clone ID Related Accession (Same CDS sequence) XM_023651567.1
    Accession Version XM_023651567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507335.1
    CDS23..5314
    Translation

    Target ORF information:

    RefSeq Version XM_023651567.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCT TGGAGGGGCT GTGTCTGCGG CGGCGGCGGC GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCTTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCTTCTTTA
    AGGCGAATTA AAGGTAGACT GCACAGAAGC AAAAGCCTTG ATAGCATCGA TTTCTGTGAA
    CTCACTAACA CAGTAATGGA GGAAACAGCT ATATGGGAAC AACACACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATCGCA ATATCTGGTG GAAGAGATAA TCCTCATTTT
    CAGAGTGGAG AAACTTCAAT AGTAATTTCA GATGTGCTGA AAGGAGGACC CGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCCATG GTTAATGGAG TTTCGATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAGA ATGCAAAAAT TACTATCCGA
    AGGAAAAAGA AAGTTCAGAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGAAAAT
    GAAGAAGATA GTTATGATGA GGAAGTACAT GATCCAAGAA GTGGCCGTGG TGGTCTGATT
    AATAGAAGGA GTGAGAAGAG TTGGGTGAGG GATAGAAGTG CAAGTAGAGA GAGGAGTTTG
    TCTCCACGAT CTGATAGGCG ATCTGTGGCC TCCAGTCAAC CTGCCAAACC CACCAAAGTC
    ACATTGGTGA AATCCCGGAA GAATGAAGAA TATGGTCTTC GATTGGCCAG CCATATATTT
    GTAAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGACG GCAATATTCA AGAAGGCGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAGTTAAAA ATGGTAGTTC AGAGAGATGA ACGGGCTACC
    CTATTGAACG TCCCTGATCT TGCTGACAGT ATCCACTCTG CTAACGCCTC TGAGAGAGAT
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCCG GTCGATCACA CGACAGGCCT
    CCTCGCCACA GCCGGTCACG ATCTCCTGAC CAACGGTCAG AGCCTTCTGA TCATTCCAGA
    CACTCTCCAC AACAGCCCAG CAATGGCAGT CTCCGGAGCA GAGAAGAAGA GAGAATTTCT
    AAACCTGGGG CCATCTCAAC TCCCGTAAAG CATGCAGATG ATCATACACC TAAAACAGTG
    GAAGAAGTTA TAGTTGAAAG AAATGAGAAA CAAGCCCCGA CTCTTCCAGA ACCAAAGCCT
    GTATATGCTC AAGTTGGACA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATC CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGCCTG GCTGGTGGAA ATGATGTCGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCCGCAGCC AAAGAAGGCT TAGAAGAGGG TGATCAAATT
    CTCAGGGTAA ACAATGTAGA TTTCACAAAC ATCATAAGAG AAGAAGCTGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGTATCG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACTCA TTTTGAATAT
    GAAAAAGAAT CTCCCTACGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTCGTGGAT
    ACATTGTACA ATGGAAAACT AGGCTCCTGG TTGGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGTTGGC CAGTGTACAA
    TACACACTTC CAAAAACAGC AGGAGGAGAC CGAGCTGACT TCTGGAGATT CCGGGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCAGCTCA GCCAGTTCAA
    ACAAAGTTTC CAGCATATGA AAGAGTTGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATCTTTG GACCAATAGC TGATGTTGCC AGGGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAGA TTGCAAAAAG TGAACCACGA GATGCTGGAA CTGACCAGCG AAGCTCTGGC
    ATTATTCGTC TTCATACAAT AAAGCAAATA ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTGAAT TACGCCCAGT GGTATCCAAT TGTAGTCTTC
    CTTAACCCTG ATTCTAAGCA AGGTGTGAAA ACTATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAATT ATATGAGCGG TCCCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTCACAA CTACAATTAA CCTAAATTCA ATGAATGATG GTTGGTATGG TGCATTAAAA
    GAAGCAATTC AACAACAACA GAACCAGTTG GTATGGGTTT CTGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCCCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAT
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAATCAAAC ATATCCTTCT TATTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAAACA GCCTCTCAAC AGGTATATAG AAAGGATCCT
    TATCCTGAGG ACATGATGAG ACAGAATCAT GTTTTGAAAC AGCCGGCTGT TGGTCACCCA
    GGGCAGAGGG CAGACAAAGA GCCTAACCTG AGCTATGAAG CCCAGCCCCC ATATGTGGAG
    AAACAAGCCA GCAGAGACCT TGAGCAGCCC ACATATAGAT ATGACTCCTC AAGCTATGCA
    GAACAGTTTT CTCGAAACTA TGACCATCGT CTACACTATG AAGATCGCAT CCCTACATAT
    GAAGAGCAGT GGTCATATTA TGATGACAAA CAGCCCTACC AACCTCGGCC TTCTTTTGAT
    AATCAGCACC CTCGAGACCT TGACTCCAGA CAGCATCCTG AAGAGTCCTC AGAACGAGGG
    TATTTCCCAC GTTTTGAAGA GCCAACTCCT CTGTCCTATG ACAGCAGACC ACGCTATGAC
    CAACCACCTA GAACCTCCAC CCTACGACAT GAAGAGCAAG CAGCTCCTGG ATATGACATA
    CATAACAGAT ACAGACCAGA AGCACAGCCC TATTCTTCAG CAGGCCATAA GGCCTCTGAG
    GCCAAGCAGT ATTTTGACCA ATACCCACGA AGTTATGAGC AAGTACCATC ACAAGGACTT
    ACCTCTAAAG CAGGCCATTA TGAGCCTCTC CATGGTGCTG CAGTTGTCAC TCCTCTAATA
    CCTGCATCTC AGCAGAAGCC GGAAGTTCTG CCTTCAAGTA CGAAGCCACT GCCTCCACCC
    CCAACTCTCA CTGAAGAAGA GGAAGATCCA GCAATGAAAC CACAGTCTGT GCTCACTAGA
    GTTAAAATGT TTGAAAACAA AAGATCTGCA TCTTTGGAGA ACAAGAAGGA TGAAAACCAC
    ACCACTAGTT TTAAGCCTCC AGAGGTAGCA TCTAAACCTC CAGTTGCTCC CATCATTGGT
    CCTAAAGCCA CTCCTCAAAA TCAGTTCAGT GAACATGACA AAACACTCTA CAGGGTCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TCCGGTCAAA TCATTATGAT
    CCTGAGGAAG ATGAAGAATA TTATCGAAAG CAGCTCTCAT ACTTTGACCG AAGAAGTTTT
    GAGAACAAGC CTTCTCCACA CATTCCTGCT AGCCATCTCT CAGAGCCTGC CAAGCCAGTT
    CCTTCTCAGA ACCAAACAAA TTTTTCTGGT TATTCTTTGA AGGGAAAGTC TACTGAAGCT
    GATGCTTTGG AGAGATCATT TGGTGAGAAA CGCTATGACC CAATCCAGGC CACTCCCCCC
    CCTCCTCCAT TGCCCTCCCA GTACACCCAG CCTTCTCAGC CCGGCTCCAG CTCTTCTCTC
    GCCCTCCACA CACATGCCAA GGGAGGACAT GGTGAAGGTA ATTCAGCATC ACTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG TCTGCTTTCT ATCCCCAGAA AAGTTTCCAA
    GATAAAGCTC CAATTAATGG AGCTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACGC CAAAACCATA TACCAGTTCT GCTCGGCCGT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAGGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAACCCCCTA CTTCTCCAAA AACTCTGGTG AAAGCACACA GTTCAGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCCATC CATGCAGATA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTATGGA AGAGGATGAA
    GATGAGGATG GTCATACCGT GGTGGCCACA GCCCGAGGCG TATTTAACAG CAATGGTGGG
    GTGTTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CACAAGGAGC CATTCCTGAA
    GGAATTGAGC AGGAAATCTA TTTCAAGGTC TGCCGAGACA ACAGCATCCT CCCGCCTTTA
    GATAAGGAGA AAGGTGAAAC ACTGCTGAGC CCCTTAGTGA TGTGTGGGCC CCATGGCCTC
    AAGTTCCTGA AGCCGGTGGA GCTACGCCTG CCACACTGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CTGGAGATCC AAATTATCTT GTTGGAGCAA ACTGTGTTTC TGTCCTGATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41623
    Clone ID Related Accession (Same CDS sequence) XM_023651568.1 , XM_023651569.1
    Accession Version XM_023651568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAACAC ACAGTGACGC TTCACAGGGC TCCTGGATTT 
    GGATTTGGAA TCGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGAGAAACT
    TCAATAGTAA TTTCAGATGT GCTGAAAGGA GGACCCGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CCATGGTTAA TGGAGTTTCG ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAACTAA GGAAAAGTGG GAAGAATGCA AAAATTACTA TCCGAAGGAA AAAGAAAGTT
    CAGATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG AAAATGAAGA AGATAGTTAT
    GATGAGGAAG TACATGATCC AAGAAGTGGC CGTGGTGGTC TGATTAATAG AAGGAGTGAG
    AAGAGTTGGG TGAGGGATAG AAGTGCAAGT AGAGAGAGGA GTTTGTCTCC ACGATCTGAT
    AGGCGATCTG TGGCCTCCAG TCAACCTGCC AAACCCACCA AAGTCACATT GGTGAAATCC
    CGGAAGAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGACGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACCCTATT GAACGTCCCT
    GATCTTGCTG ACAGTATCCA CTCTGCTAAC GCCTCTGAGA GAGATGACAT TTCAGAAATT
    CAGTCACTGG CATCAGATCA TTCCGGTCGA TCACACGACA GGCCTCCTCG CCACAGCCGG
    TCACGATCTC CTGACCAACG GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAACAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAA GAAGAGAGAA TTTCTAAACC TGGGGCCATC
    TCAACTCCCG TAAAGCATGC AGATGATCAT ACACCTAAAA CAGTGGAAGA AGTTATAGTT
    GAAAGAAATG AGAAACAAGC CCCGACTCTT CCAGAACCAA AGCCTGTATA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCTGTCAGT CCATCTGATG GTGTCCTACC TAATTCAACT
    CATGAAGATG GGATCCTTCG GCCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GCCTGGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCCG CAGCCAAAGA AGGCTTAGAA GAGGGTGATC AAATTCTCAG GGTAAACAAT
    GTAGATTTCA CAAACATCAT AAGAGAAGAA GCTGTCCTTT TCCTGCTTGA CCTCCCTAAA
    GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG TATCGTAGAA
    TCAGATGTAG GAGATTCTTT CTATATTAGA ACTCATTTTG AATATGAAAA AGAATCTCCC
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTCG TGGATACATT GTACAATGGA
    AAACTAGGCT CCTGGTTGGC TATTCGAATT GGTAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTGGCCAGTG TACAATACAC ACTTCCAAAA
    ACAGCAGGAG GAGACCGAGC TGACTTCTGG AGATTCCGGG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGATTTGTCA GCTCAGCCAG TTCAAACAAA GTTTCCAGCA
    TATGAAAGAG TTGTTCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGGGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CAGCGAAGCT CTGGCATTAT TCGTCTTCAT
    ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA
    GTTGATCGTC TGAATTACGC CCAGTGGTAT CCAATTGTAG TCTTCCTTAA CCCTGATTCT
    AAGCAAGGTG TGAAAACTAT GAGAATGAGG TTATGTCCAG AATCTCGGAA AAGTGCCAGG
    AAATTATATG AGCGGTCCCA TAAACTTCGT AAAAATAATC ACCATCTTTT CACAACTACA
    ATTAACCTAA ATTCAATGAA TGATGGTTGG TATGGTGCAT TAAAAGAAGC AATTCAACAA
    CAACAGAACC AGTTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAT
    CAAACATATC CTTCTTATTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC
    CCTGGATTTA AAACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTATCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT
    AATGTCAGAC TGGAGGAGCT CACCCCAGCT CCTCCCACCT CTTACTCACC ACAAGCTGAT
    TCTTTAAGAA CACCAAGTTC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAAGCATCA
    CTGTCGTCGC ATGTAGATCC AACAAAGGTA TATAGAAAGG ATCCTTATCC TGAGGACATG
    ATGAGACAGA ATCATGTTTT GAAACAGCCG GCTGTTGGTC ACCCAGGGCA GAGGGCAGAC
    AAAGAGCCTA ACCTGAGCTA TGAAGCCCAG CCCCCATATG TGGAGAAACA AGCCAGCAGA
    GACCTTGAGC AGCCCACATA TAGATATGAC TCCTCAAGCT ATGCAGAACA GTTTTCTCGA
    AACTATGACC ATCGTCTACA CTATGAAGAT CGCATCCCTA CATATGAAGA GCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTCTT TTGATAATCA GCACCCTCGA
    GACCTTGACT CCAGACAGCA TCCTGAAGAG TCCTCAGAAC GAGGGTATTT CCCACGTTTT
    GAAGAGCCAA CTCCTCTGTC CTATGACAGC AGACCACGCT ATGACCAACC ACCTAGAACC
    TCCACCCTAC GACATGAAGA GCAAGCAGCT CCTGGATATG ACATACATAA CAGATACAGA
    CCAGAAGCAC AGCCCTATTC TTCAGCAGGC CATAAGGCCT CTGAGGCCAA GCAGTATTTT
    GACCAATACC CACGAAGTTA TGAGCAAGTA CCATCACAAG GACTTACCTC TAAAGCAGGC
    CATTATGAGC CTCTCCATGG TGCTGCAGTT GTCACTCCTC TAATACCTGC ATCTCAGCAG
    AAGCCGGAAG TTCTGCCTTC AAGTACGAAG CCACTGCCTC CACCCCCAAC TCTCACTGAA
    GAAGAGGAAG ATCCAGCAAT GAAACCACAG TCTGTGCTCA CTAGAGTTAA AATGTTTGAA
    AACAAAAGAT CTGCATCTTT GGAGAACAAG AAGGATGAAA ACCACACCAC TAGTTTTAAG
    CCTCCAGAGG TAGCATCTAA ACCTCCAGTT GCTCCCATCA TTGGTCCTAA AGCCACTCCT
    CAAAATCAGT TCAGTGAACA TGACAAAACA CTCTACAGGG TCCCAGAACC TCAAAAACCT
    CAACTGAAGC CACCTGAAGA TATTGTCCGG TCAAATCATT ATGATCCTGA GGAAGATGAA
    GAATATTATC GAAAGCAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA CAAGCCTTCT
    CCACACATTC CTGCTAGCCA TCTCTCAGAG CCTGCCAAGC CAGTTCCTTC TCAGAACCAA
    ACAAATTTTT CTGGTTATTC TTTGAAGGGA AAGTCTACTG AAGCTGATGC TTTGGAGAGA
    TCATTTGGTG AGAAACGCTA TGACCCAATC CAGGCCACTC CCCCCCCTCC TCCATTGCCC
    TCCCAGTACA CCCAGCCTTC TCAGCCCGGC TCCAGCTCTT CTCTCGCCCT CCACACACAT
    GCCAAGGGAG GACATGGTGA AGGTAATTCA GCATCACTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGTCTGC TTTCTATCCC CAGAAAAGTT TCCAAGATAA AGCTCCAATT
    AATGGAGCTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACGCCAAAA
    CCATATACCA GTTCTGCTCG GCCGTTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAGGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAACC CCCTACTTCT
    CCAAAAACTC TGGTGAAAGC ACACAGTTCA GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CCATCCATGC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT ATGGAAGAGG ATGAAGATGA GGATGGTCAT
    ACCGTGGTGG CCACAGCCCG AGGCGTATTT AACAGCAATG GTGGGGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG AGACAACAGC ATCCTCCCGC CTTTAGATAA GGAGAAAGGT
    GAAACACTGC TGAGCCCCTT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCG
    GTGGAGCTAC GCCTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCAAATT ATCTTGTTGG AGCAAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507336.1
    CDS194..5470
    Translation

    Target ORF information:

    RefSeq Version XM_023651568.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAACAC ACAGTGACGC TTCACAGGGC TCCTGGATTT 
    GGATTTGGAA TCGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGAGAAACT
    TCAATAGTAA TTTCAGATGT GCTGAAAGGA GGACCCGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CCATGGTTAA TGGAGTTTCG ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAACTAA GGAAAAGTGG GAAGAATGCA AAAATTACTA TCCGAAGGAA AAAGAAAGTT
    CAGATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG AAAATGAAGA AGATAGTTAT
    GATGAGGAAG TACATGATCC AAGAAGTGGC CGTGGTGGTC TGATTAATAG AAGGAGTGAG
    AAGAGTTGGG TGAGGGATAG AAGTGCAAGT AGAGAGAGGA GTTTGTCTCC ACGATCTGAT
    AGGCGATCTG TGGCCTCCAG TCAACCTGCC AAACCCACCA AAGTCACATT GGTGAAATCC
    CGGAAGAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGACGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACCCTATT GAACGTCCCT
    GATCTTGCTG ACAGTATCCA CTCTGCTAAC GCCTCTGAGA GAGATGACAT TTCAGAAATT
    CAGTCACTGG CATCAGATCA TTCCGGTCGA TCACACGACA GGCCTCCTCG CCACAGCCGG
    TCACGATCTC CTGACCAACG GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAACAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAA GAAGAGAGAA TTTCTAAACC TGGGGCCATC
    TCAACTCCCG TAAAGCATGC AGATGATCAT ACACCTAAAA CAGTGGAAGA AGTTATAGTT
    GAAAGAAATG AGAAACAAGC CCCGACTCTT CCAGAACCAA AGCCTGTATA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCTGTCAGT CCATCTGATG GTGTCCTACC TAATTCAACT
    CATGAAGATG GGATCCTTCG GCCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GCCTGGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCCG CAGCCAAAGA AGGCTTAGAA GAGGGTGATC AAATTCTCAG GGTAAACAAT
    GTAGATTTCA CAAACATCAT AAGAGAAGAA GCTGTCCTTT TCCTGCTTGA CCTCCCTAAA
    GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG TATCGTAGAA
    TCAGATGTAG GAGATTCTTT CTATATTAGA ACTCATTTTG AATATGAAAA AGAATCTCCC
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTCG TGGATACATT GTACAATGGA
    AAACTAGGCT CCTGGTTGGC TATTCGAATT GGTAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTGGCCAGTG TACAATACAC ACTTCCAAAA
    ACAGCAGGAG GAGACCGAGC TGACTTCTGG AGATTCCGGG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGATTTGTCA GCTCAGCCAG TTCAAACAAA GTTTCCAGCA
    TATGAAAGAG TTGTTCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGGGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CAGCGAAGCT CTGGCATTAT TCGTCTTCAT
    ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA
    GTTGATCGTC TGAATTACGC CCAGTGGTAT CCAATTGTAG TCTTCCTTAA CCCTGATTCT
    AAGCAAGGTG TGAAAACTAT GAGAATGAGG TTATGTCCAG AATCTCGGAA AAGTGCCAGG
    AAATTATATG AGCGGTCCCA TAAACTTCGT AAAAATAATC ACCATCTTTT CACAACTACA
    ATTAACCTAA ATTCAATGAA TGATGGTTGG TATGGTGCAT TAAAAGAAGC AATTCAACAA
    CAACAGAACC AGTTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAT
    CAAACATATC CTTCTTATTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC
    CCTGGATTTA AAACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTATCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT
    AATGTCAGAC TGGAGGAGCT CACCCCAGCT CCTCCCACCT CTTACTCACC ACAAGCTGAT
    TCTTTAAGAA CACCAAGTTC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAAGCATCA
    CTGTCGTCGC ATGTAGATCC AACAAAGGTA TATAGAAAGG ATCCTTATCC TGAGGACATG
    ATGAGACAGA ATCATGTTTT GAAACAGCCG GCTGTTGGTC ACCCAGGGCA GAGGGCAGAC
    AAAGAGCCTA ACCTGAGCTA TGAAGCCCAG CCCCCATATG TGGAGAAACA AGCCAGCAGA
    GACCTTGAGC AGCCCACATA TAGATATGAC TCCTCAAGCT ATGCAGAACA GTTTTCTCGA
    AACTATGACC ATCGTCTACA CTATGAAGAT CGCATCCCTA CATATGAAGA GCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTCTT TTGATAATCA GCACCCTCGA
    GACCTTGACT CCAGACAGCA TCCTGAAGAG TCCTCAGAAC GAGGGTATTT CCCACGTTTT
    GAAGAGCCAA CTCCTCTGTC CTATGACAGC AGACCACGCT ATGACCAACC ACCTAGAACC
    TCCACCCTAC GACATGAAGA GCAAGCAGCT CCTGGATATG ACATACATAA CAGATACAGA
    CCAGAAGCAC AGCCCTATTC TTCAGCAGGC CATAAGGCCT CTGAGGCCAA GCAGTATTTT
    GACCAATACC CACGAAGTTA TGAGCAAGTA CCATCACAAG GACTTACCTC TAAAGCAGGC
    CATTATGAGC CTCTCCATGG TGCTGCAGTT GTCACTCCTC TAATACCTGC ATCTCAGCAG
    AAGCCGGAAG TTCTGCCTTC AAGTACGAAG CCACTGCCTC CACCCCCAAC TCTCACTGAA
    GAAGAGGAAG ATCCAGCAAT GAAACCACAG TCTGTGCTCA CTAGAGTTAA AATGTTTGAA
    AACAAAAGAT CTGCATCTTT GGAGAACAAG AAGGATGAAA ACCACACCAC TAGTTTTAAG
    CCTCCAGAGG TAGCATCTAA ACCTCCAGTT GCTCCCATCA TTGGTCCTAA AGCCACTCCT
    CAAAATCAGT TCAGTGAACA TGACAAAACA CTCTACAGGG TCCCAGAACC TCAAAAACCT
    CAACTGAAGC CACCTGAAGA TATTGTCCGG TCAAATCATT ATGATCCTGA GGAAGATGAA
    GAATATTATC GAAAGCAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA CAAGCCTTCT
    CCACACATTC CTGCTAGCCA TCTCTCAGAG CCTGCCAAGC CAGTTCCTTC TCAGAACCAA
    ACAAATTTTT CTGGTTATTC TTTGAAGGGA AAGTCTACTG AAGCTGATGC TTTGGAGAGA
    TCATTTGGTG AGAAACGCTA TGACCCAATC CAGGCCACTC CCCCCCCTCC TCCATTGCCC
    TCCCAGTACA CCCAGCCTTC TCAGCCCGGC TCCAGCTCTT CTCTCGCCCT CCACACACAT
    GCCAAGGGAG GACATGGTGA AGGTAATTCA GCATCACTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGTCTGC TTTCTATCCC CAGAAAAGTT TCCAAGATAA AGCTCCAATT
    AATGGAGCTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACGCCAAAA
    CCATATACCA GTTCTGCTCG GCCGTTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAGGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAACC CCCTACTTCT
    CCAAAAACTC TGGTGAAAGC ACACAGTTCA GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CCATCCATGC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT ATGGAAGAGG ATGAAGATGA GGATGGTCAT
    ACCGTGGTGG CCACAGCCCG AGGCGTATTT AACAGCAATG GTGGGGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG AGACAACAGC ATCCTCCCGC CTTTAGATAA GGAGAAAGGT
    GAAACACTGC TGAGCCCCTT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCG
    GTGGAGCTAC GCCTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCAAATT ATCTTGTTGG AGCAAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb41623
    Clone ID Related Accession (Same CDS sequence) XM_023651568.1 , XM_023651569.1
    Accession Version XM_023651569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009144.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAACAC ACAGTGACGC TTCACAGGGC TCCTGGATTT 
    GGATTTGGAA TCGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGAGAAACT
    TCAATAGTAA TTTCAGATGT GCTGAAAGGA GGACCCGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CCATGGTTAA TGGAGTTTCG ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAACTAA GGAAAAGTGG GAAGAATGCA AAAATTACTA TCCGAAGGAA AAAGAAAGTT
    CAGATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG AAAATGAAGA AGATAGTTAT
    GATGAGGAAG TACATGATCC AAGAAGTGGC CGTGGTGGTC TGATTAATAG AAGGAGTGAG
    AAGAGTTGGG TGAGGGATAG AAGTGCAAGT AGAGAGAGGA GTTTGTCTCC ACGATCTGAT
    AGGCGATCTG TGGCCTCCAG TCAACCTGCC AAACCCACCA AAGTCACATT GGTGAAATCC
    CGGAAGAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGACGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACCCTATT GAACGTCCCT
    GATCTTGCTG ACAGTATCCA CTCTGCTAAC GCCTCTGAGA GAGATGACAT TTCAGAAATT
    CAGTCACTGG CATCAGATCA TTCCGGTCGA TCACACGACA GGCCTCCTCG CCACAGCCGG
    TCACGATCTC CTGACCAACG GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAACAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAA GAAGAGAGAA TTTCTAAACC TGGGGCCATC
    TCAACTCCCG TAAAGCATGC AGATGATCAT ACACCTAAAA CAGTGGAAGA AGTTATAGTT
    GAAAGAAATG AGAAACAAGC CCCGACTCTT CCAGAACCAA AGCCTGTATA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCTGTCAGT CCATCTGATG GTGTCCTACC TAATTCAACT
    CATGAAGATG GGATCCTTCG GCCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GCCTGGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCCG CAGCCAAAGA AGGCTTAGAA GAGGGTGATC AAATTCTCAG GGTAAACAAT
    GTAGATTTCA CAAACATCAT AAGAGAAGAA GCTGTCCTTT TCCTGCTTGA CCTCCCTAAA
    GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG TATCGTAGAA
    TCAGATGTAG GAGATTCTTT CTATATTAGA ACTCATTTTG AATATGAAAA AGAATCTCCC
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTCG TGGATACATT GTACAATGGA
    AAACTAGGCT CCTGGTTGGC TATTCGAATT GGTAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTGGCCAGTG TACAATACAC ACTTCCAAAA
    ACAGCAGGAG GAGACCGAGC TGACTTCTGG AGATTCCGGG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGATTTGTCA GCTCAGCCAG TTCAAACAAA GTTTCCAGCA
    TATGAAAGAG TTGTTCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGGGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CAGCGAAGCT CTGGCATTAT TCGTCTTCAT
    ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA
    GTTGATCGTC TGAATTACGC CCAGTGGTAT CCAATTGTAG TCTTCCTTAA CCCTGATTCT
    AAGCAAGGTG TGAAAACTAT GAGAATGAGG TTATGTCCAG AATCTCGGAA AAGTGCCAGG
    AAATTATATG AGCGGTCCCA TAAACTTCGT AAAAATAATC ACCATCTTTT CACAACTACA
    ATTAACCTAA ATTCAATGAA TGATGGTTGG TATGGTGCAT TAAAAGAAGC AATTCAACAA
    CAACAGAACC AGTTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAT
    CAAACATATC CTTCTTATTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC
    CCTGGATTTA AAACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTATCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT
    AATGTCAGAC TGGAGGAGCT CACCCCAGCT CCTCCCACCT CTTACTCACC ACAAGCTGAT
    TCTTTAAGAA CACCAAGTTC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAAGCATCA
    CTGTCGTCGC ATGTAGATCC AACAAAGGTA TATAGAAAGG ATCCTTATCC TGAGGACATG
    ATGAGACAGA ATCATGTTTT GAAACAGCCG GCTGTTGGTC ACCCAGGGCA GAGGGCAGAC
    AAAGAGCCTA ACCTGAGCTA TGAAGCCCAG CCCCCATATG TGGAGAAACA AGCCAGCAGA
    GACCTTGAGC AGCCCACATA TAGATATGAC TCCTCAAGCT ATGCAGAACA GTTTTCTCGA
    AACTATGACC ATCGTCTACA CTATGAAGAT CGCATCCCTA CATATGAAGA GCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTCTT TTGATAATCA GCACCCTCGA
    GACCTTGACT CCAGACAGCA TCCTGAAGAG TCCTCAGAAC GAGGGTATTT CCCACGTTTT
    GAAGAGCCAA CTCCTCTGTC CTATGACAGC AGACCACGCT ATGACCAACC ACCTAGAACC
    TCCACCCTAC GACATGAAGA GCAAGCAGCT CCTGGATATG ACATACATAA CAGATACAGA
    CCAGAAGCAC AGCCCTATTC TTCAGCAGGC CATAAGGCCT CTGAGGCCAA GCAGTATTTT
    GACCAATACC CACGAAGTTA TGAGCAAGTA CCATCACAAG GACTTACCTC TAAAGCAGGC
    CATTATGAGC CTCTCCATGG TGCTGCAGTT GTCACTCCTC TAATACCTGC ATCTCAGCAG
    AAGCCGGAAG TTCTGCCTTC AAGTACGAAG CCACTGCCTC CACCCCCAAC TCTCACTGAA
    GAAGAGGAAG ATCCAGCAAT GAAACCACAG TCTGTGCTCA CTAGAGTTAA AATGTTTGAA
    AACAAAAGAT CTGCATCTTT GGAGAACAAG AAGGATGAAA ACCACACCAC TAGTTTTAAG
    CCTCCAGAGG TAGCATCTAA ACCTCCAGTT GCTCCCATCA TTGGTCCTAA AGCCACTCCT
    CAAAATCAGT TCAGTGAACA TGACAAAACA CTCTACAGGG TCCCAGAACC TCAAAAACCT
    CAACTGAAGC CACCTGAAGA TATTGTCCGG TCAAATCATT ATGATCCTGA GGAAGATGAA
    GAATATTATC GAAAGCAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA CAAGCCTTCT
    CCACACATTC CTGCTAGCCA TCTCTCAGAG CCTGCCAAGC CAGTTCCTTC TCAGAACCAA
    ACAAATTTTT CTGGTTATTC TTTGAAGGGA AAGTCTACTG AAGCTGATGC TTTGGAGAGA
    TCATTTGGTG AGAAACGCTA TGACCCAATC CAGGCCACTC CCCCCCCTCC TCCATTGCCC
    TCCCAGTACA CCCAGCCTTC TCAGCCCGGC TCCAGCTCTT CTCTCGCCCT CCACACACAT
    GCCAAGGGAG GACATGGTGA AGGTAATTCA GCATCACTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGTCTGC TTTCTATCCC CAGAAAAGTT TCCAAGATAA AGCTCCAATT
    AATGGAGCTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACGCCAAAA
    CCATATACCA GTTCTGCTCG GCCGTTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAGGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAACC CCCTACTTCT
    CCAAAAACTC TGGTGAAAGC ACACAGTTCA GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CCATCCATGC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT ATGGAAGAGG ATGAAGATGA GGATGGTCAT
    ACCGTGGTGG CCACAGCCCG AGGCGTATTT AACAGCAATG GTGGGGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG AGACAACAGC ATCCTCCCGC CTTTAGATAA GGAGAAAGGT
    GAAACACTGC TGAGCCCCTT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCG
    GTGGAGCTAC GCCTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCAAATT ATCTTGTTGG AGCAAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023507337.1
    CDS123..5399
    Translation

    Target ORF information:

    RefSeq Version XM_023651569.1
    Organism Equus caballus(horse)
    Definition Equus caballus tight junction protein 1 (TJP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023651569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGGAAA CAGCTATATG GGAACAACAC ACAGTGACGC TTCACAGGGC TCCTGGATTT 
    GGATTTGGAA TCGCAATATC TGGTGGAAGA GATAATCCTC ATTTTCAGAG TGGAGAAACT
    TCAATAGTAA TTTCAGATGT GCTGAAAGGA GGACCCGCTG AAGGACAGCT ACAGGAAAAT
    GACCGAGTTG CCATGGTTAA TGGAGTTTCG ATGGATAATG TTGAACATGC TTTTGCTGTT
    CAGCAACTAA GGAAAAGTGG GAAGAATGCA AAAATTACTA TCCGAAGGAA AAAGAAAGTT
    CAGATACCAG TAAGTCGTCC TGATCCTGAA CCAGTATCTG AAAATGAAGA AGATAGTTAT
    GATGAGGAAG TACATGATCC AAGAAGTGGC CGTGGTGGTC TGATTAATAG AAGGAGTGAG
    AAGAGTTGGG TGAGGGATAG AAGTGCAAGT AGAGAGAGGA GTTTGTCTCC ACGATCTGAT
    AGGCGATCTG TGGCCTCCAG TCAACCTGCC AAACCCACCA AAGTCACATT GGTGAAATCC
    CGGAAGAATG AAGAATATGG TCTTCGATTG GCCAGCCATA TATTTGTAAA GGAAATTTCA
    CAAGATAGTT TGGCAGCAAG AGACGGCAAT ATTCAAGAAG GCGATGTTGT ATTGAAGATA
    AATGGTACTG TGACAGAAAA TATGTCATTG ACAGATGCAA AGACATTGAT AGAAAGGTCT
    AAAGGCAAGT TAAAAATGGT AGTTCAGAGA GATGAACGGG CTACCCTATT GAACGTCCCT
    GATCTTGCTG ACAGTATCCA CTCTGCTAAC GCCTCTGAGA GAGATGACAT TTCAGAAATT
    CAGTCACTGG CATCAGATCA TTCCGGTCGA TCACACGACA GGCCTCCTCG CCACAGCCGG
    TCACGATCTC CTGACCAACG GTCAGAGCCT TCTGATCATT CCAGACACTC TCCACAACAG
    CCCAGCAATG GCAGTCTCCG GAGCAGAGAA GAAGAGAGAA TTTCTAAACC TGGGGCCATC
    TCAACTCCCG TAAAGCATGC AGATGATCAT ACACCTAAAA CAGTGGAAGA AGTTATAGTT
    GAAAGAAATG AGAAACAAGC CCCGACTCTT CCAGAACCAA AGCCTGTATA TGCTCAAGTT
    GGACAACCAG ATGTGGATTT ACCTGTCAGT CCATCTGATG GTGTCCTACC TAATTCAACT
    CATGAAGATG GGATCCTTCG GCCCAGCATG AAATTGGTAA AATTCAGAAA AGGAGATAGT
    GTGGGTTTGC GCCTGGCTGG TGGAAATGAT GTCGGAATAT TTGTAGCTGG CGTTCTAGAA
    GATAGCCCCG CAGCCAAAGA AGGCTTAGAA GAGGGTGATC AAATTCTCAG GGTAAACAAT
    GTAGATTTCA CAAACATCAT AAGAGAAGAA GCTGTCCTTT TCCTGCTTGA CCTCCCTAAA
    GGAGAAGAAG TGACCATATT GGCTCAGAAG AAGAAGGATG TTTATCGTCG TATCGTAGAA
    TCAGATGTAG GAGATTCTTT CTATATTAGA ACTCATTTTG AATATGAAAA AGAATCTCCC
    TACGGACTTA GTTTTAACAA AGGAGAGGTG TTCCGTGTCG TGGATACATT GTACAATGGA
    AAACTAGGCT CCTGGTTGGC TATTCGAATT GGTAAAAATC ATAAGGAGGT AGAACGAGGC
    ATCATCCCTA ATAAGAACAG AGCTGAGCAG TTGGCCAGTG TACAATACAC ACTTCCAAAA
    ACAGCAGGAG GAGACCGAGC TGACTTCTGG AGATTCCGGG GTCTTCGCAG CTCCAAGAGA
    AATCTTCGAA AAAGCAGAGA GGATTTGTCA GCTCAGCCAG TTCAAACAAA GTTTCCAGCA
    TATGAAAGAG TTGTTCTTCG AGAAGCTGGA TTTCTGAGGC CTGTAACCAT CTTTGGACCA
    ATAGCTGATG TTGCCAGGGA AAAGCTGGCA AGAGAAGAAC CAGATATTTA TCAGATTGCA
    AAAAGTGAAC CACGAGATGC TGGAACTGAC CAGCGAAGCT CTGGCATTAT TCGTCTTCAT
    ACAATAAAGC AAATAATAGA TCAAGACAAA CATGCTTTAT TAGATGTAAC ACCAAATGCA
    GTTGATCGTC TGAATTACGC CCAGTGGTAT CCAATTGTAG TCTTCCTTAA CCCTGATTCT
    AAGCAAGGTG TGAAAACTAT GAGAATGAGG TTATGTCCAG AATCTCGGAA AAGTGCCAGG
    AAATTATATG AGCGGTCCCA TAAACTTCGT AAAAATAATC ACCATCTTTT CACAACTACA
    ATTAACCTAA ATTCAATGAA TGATGGTTGG TATGGTGCAT TAAAAGAAGC AATTCAACAA
    CAACAGAACC AGTTGGTATG GGTTTCTGAG GGAAAGGCGG ATGGTGCTAC AAGTGATGAC
    CTTGATTTGC ATGATGATCG TCTGTCCTAC CTGTCAGCCC CAGGTAGTGA ATACTCAATG
    TATAGCACGG ACAGTAGACA CACTTCTGAC TATGAAGACA CAGATACAGA AGGCGGGGCC
    TACACTGATC AAGAACTAGA TGAAACTCTT AATGATGAGG TTGGGACTCC ACCGGAGTCT
    GCCATTACAC GGTCCTCTGA GCCTGTAAGA GAGGACTCCT CTGGAATGCA TCATGAAAAT
    CAAACATATC CTTCTTATTC ACCACAAGCG CAGCCACAAC CAATTCATAG AATAGACTCC
    CCTGGATTTA AAACAGCCTC TCAACAGAAA GCAGAAGCCT CATCTCCAGT CCCTTATCTT
    TCGCCTGAAA CAAACCCAGC ATCATCAACC TCTGCTGTTA ATCATAATGT AAATTTAACT
    AATGTCAGAC TGGAGGAGCT CACCCCAGCT CCTCCCACCT CTTACTCACC ACAAGCTGAT
    TCTTTAAGAA CACCAAGTTC TGAGGCAGCT CACATAATGC TAAGAGATCA AGAAGCATCA
    CTGTCGTCGC ATGTAGATCC AACAAAGGTA TATAGAAAGG ATCCTTATCC TGAGGACATG
    ATGAGACAGA ATCATGTTTT GAAACAGCCG GCTGTTGGTC ACCCAGGGCA GAGGGCAGAC
    AAAGAGCCTA ACCTGAGCTA TGAAGCCCAG CCCCCATATG TGGAGAAACA AGCCAGCAGA
    GACCTTGAGC AGCCCACATA TAGATATGAC TCCTCAAGCT ATGCAGAACA GTTTTCTCGA
    AACTATGACC ATCGTCTACA CTATGAAGAT CGCATCCCTA CATATGAAGA GCAGTGGTCA
    TATTATGATG ACAAACAGCC CTACCAACCT CGGCCTTCTT TTGATAATCA GCACCCTCGA
    GACCTTGACT CCAGACAGCA TCCTGAAGAG TCCTCAGAAC GAGGGTATTT CCCACGTTTT
    GAAGAGCCAA CTCCTCTGTC CTATGACAGC AGACCACGCT ATGACCAACC ACCTAGAACC
    TCCACCCTAC GACATGAAGA GCAAGCAGCT CCTGGATATG ACATACATAA CAGATACAGA
    CCAGAAGCAC AGCCCTATTC TTCAGCAGGC CATAAGGCCT CTGAGGCCAA GCAGTATTTT
    GACCAATACC CACGAAGTTA TGAGCAAGTA CCATCACAAG GACTTACCTC TAAAGCAGGC
    CATTATGAGC CTCTCCATGG TGCTGCAGTT GTCACTCCTC TAATACCTGC ATCTCAGCAG
    AAGCCGGAAG TTCTGCCTTC AAGTACGAAG CCACTGCCTC CACCCCCAAC TCTCACTGAA
    GAAGAGGAAG ATCCAGCAAT GAAACCACAG TCTGTGCTCA CTAGAGTTAA AATGTTTGAA
    AACAAAAGAT CTGCATCTTT GGAGAACAAG AAGGATGAAA ACCACACCAC TAGTTTTAAG
    CCTCCAGAGG TAGCATCTAA ACCTCCAGTT GCTCCCATCA TTGGTCCTAA AGCCACTCCT
    CAAAATCAGT TCAGTGAACA TGACAAAACA CTCTACAGGG TCCCAGAACC TCAAAAACCT
    CAACTGAAGC CACCTGAAGA TATTGTCCGG TCAAATCATT ATGATCCTGA GGAAGATGAA
    GAATATTATC GAAAGCAGCT CTCATACTTT GACCGAAGAA GTTTTGAGAA CAAGCCTTCT
    CCACACATTC CTGCTAGCCA TCTCTCAGAG CCTGCCAAGC CAGTTCCTTC TCAGAACCAA
    ACAAATTTTT CTGGTTATTC TTTGAAGGGA AAGTCTACTG AAGCTGATGC TTTGGAGAGA
    TCATTTGGTG AGAAACGCTA TGACCCAATC CAGGCCACTC CCCCCCCTCC TCCATTGCCC
    TCCCAGTACA CCCAGCCTTC TCAGCCCGGC TCCAGCTCTT CTCTCGCCCT CCACACACAT
    GCCAAGGGAG GACATGGTGA AGGTAATTCA GCATCACTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGTCTGC TTTCTATCCC CAGAAAAGTT TCCAAGATAA AGCTCCAATT
    AATGGAGCTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACGCCAAAA
    CCATATACCA GTTCTGCTCG GCCGTTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAGGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAACC CCCTACTTCT
    CCAAAAACTC TGGTGAAAGC ACACAGTTCA GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CCATCCATGC AGATAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT ATGGAAGAGG ATGAAGATGA GGATGGTCAT
    ACCGTGGTGG CCACAGCCCG AGGCGTATTT AACAGCAATG GTGGGGTGTT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCACAA GGAGCCATTC CTGAAGGAAT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG AGACAACAGC ATCCTCCCGC CTTTAGATAA GGAGAAAGGT
    GAAACACTGC TGAGCCCCTT AGTGATGTGT GGGCCCCATG GCCTCAAGTT CCTGAAGCCG
    GTGGAGCTAC GCCTGCCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCTGGA
    GATCCAAATT ATCTTGTTGG AGCAAACTGT GTTTCTGTCC TGATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.