MID1 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID1
Entrez Gene ID	100049999
Full Name	midline 1
General protein information	Preferred Names midline 1 Names E3 ubiquitin-protein ligase Midline-1 midline 1 (Opitz/BBB syndrome)
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005613975.3	XP_005614032.1	E3 ubiquitin-protein ligase Midline-1
XM_001488111.4	XP_001488161.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
XM_005613975.2	XP_005614032.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_005613976.3	XP_005614033.1	E3 ubiquitin-protein ligase Midline-1
XM_014728758.1	XP_014584244.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_005613976.2	XP_005614033.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_014728758.2	XP_014584244.1	E3 ubiquitin-protein ligase Midline-1
XM_023633600.1	XP_023489368.1	E3 ubiquitin-protein ligase Midline-1

Pathways from BioSystems
Gene Ontology

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb26666	XM_023633600.1 Latest version!	Equus caballus midline 1 (MID1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHb26666	XM_005613975.3 Latest version!	Equus caballus midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHb26666	XM_014728758.2 Latest version!	Equus caballus midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHb26666	XM_005613976.3 Latest version!	Equus caballus midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHb26667	XM_001488111.4 Latest version!	Equus caballus midline 1 (MID1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHb26666	XM_005613975.2	Equus caballus midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHb26666	XM_005613976.2	Equus caballus midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHb26666	XM_014728758.1	Equus caballus midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID1 ( XM_005613975.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005614032.1 Equus caballus midline 1 (MID1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb26666
Clone ID Related Accession (Same CDS sequence)	XM_005613975.3 , XM_005613975.2 , XM_005613976.3 , XM_014728758.1 , XM_005613976.2 , XM_014728758.2 , XM_023633600.1
Accession Version	XM_005613975.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005613975.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005614032.1
CDS	169..2172
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005613975.3
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005613975.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005613975.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005614032.1 Equus caballus midline 1 (MID1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb26666
Clone ID Related Accession (Same CDS sequence)	XM_005613975.3 , XM_005613975.2 , XM_005613976.3 , XM_014728758.1 , XM_005613976.2 , XM_014728758.2 , XM_023633600.1
Accession Version	XM_005613975.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001877040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005613975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005614032.1
CDS	166..2169
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005613975.2
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005613975.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005613976.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005614033.1 Equus caballus midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb26666
Clone ID Related Accession (Same CDS sequence)	XM_005613975.3 , XM_005613975.2 , XM_005613976.3 , XM_014728758.1 , XM_005613976.2 , XM_014728758.2 , XM_023633600.1
Accession Version	XM_005613976.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005613976.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005614033.1
CDS	256..2259
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005613976.3
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005613976.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014728758.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014584244.1 Equus caballus midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb26666
Clone ID Related Accession (Same CDS sequence)	XM_005613975.3 , XM_005613975.2 , XM_005613976.3 , XM_014728758.1 , XM_005613976.2 , XM_014728758.2 , XM_023633600.1
Accession Version	XM_014728758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001877040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014584244.1
CDS	321..2324
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014728758.1
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014728758.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005613976.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005614033.1 Equus caballus midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb26666
Clone ID Related Accession (Same CDS sequence)	XM_005613975.3 , XM_005613975.2 , XM_005613976.3 , XM_014728758.1 , XM_005613976.2 , XM_014728758.2 , XM_023633600.1
Accession Version	XM_005613976.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001877040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005613976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005614033.1
CDS	215..2218
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005613976.2
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005613976.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_014728758.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_014584244.1 Equus caballus midline 1 (MID1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb26666
Clone ID Related Accession (Same CDS sequence)	XM_005613975.3 , XM_005613975.2 , XM_005613976.3 , XM_014728758.1 , XM_005613976.2 , XM_014728758.2 , XM_023633600.1
Accession Version	XM_014728758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014728758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014584244.1
CDS	547..2550
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_014728758.2
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014728758.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_023633600.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023489368.1 Equus caballus midline 1 (MID1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHb26666
Clone ID Related Accession (Same CDS sequence)	XM_005613975.3 , XM_005613975.2 , XM_005613976.3 , XM_014728758.1 , XM_005613976.2 , XM_014728758.2 , XM_023633600.1
Accession Version	XM_023633600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009175.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023489368.1
CDS	246..2249
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_023633600.1
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023633600.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTCTG TGTAACTCAG CCGATAGCTG GATGATCGTG 
CCCAACATCA AGCAGAACCA CTACACAGTC CATGGTTTGC AGAGCGGCAC CAAATACATC 
TTCATTGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAATAGCC AACCATTTAA ACTGGACCCC AAATCTGCAC ATCGAAAGCT AAAAGTGTCA 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AAAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAAGGGAG CTATGGCGTA GCTGGAAACG TGTTCATTGA TAGTGGCCGG 
CATTACTGGG AAGTGGTCAT AAGTGGAAGT ACCTGGTATG CCATTGGCCT TGCTTACAAA 
TCAGCCCCGA AGCACGAGTG GATTGGGAAG AACTCTGCTT CCTGGGCACT GTGTCGATGT 
CACAATAACT GGGTGGTAAG ACACAACAGC AAGGAAATCC CCATTGAGCC AGCCCCCCAC 
CTCCGGCGCG TTGGCATCCT GCTAGACTAT GATAACGGCT CCATCGCTTT CTACGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTTGAC ATCACATTCT CGCAGCCTGT GTGCCCCACC 
TTCACCGTGT GGAACAAATG CTTGACCATT ATAACTGGCC TTCCCATCCC AGACCATCTG 
GACTGCACAG AGCAACTGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_001488111.4 ) cDNA ORF clone, Equus caballus(horse) -> XP_001488161.1 Equus caballus midline 1 (MID1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHb26667
Clone ID Related Accession (Same CDS sequence)	XM_001488111.4
Accession Version	XM_001488111.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001877040.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001488111.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTGAA GAGTGGGTTT CTTGGGGCCC AGAGCTCAGA 
CCATGTAAGG AGGGAATATC ATGCTTGCGC CAAAGAACTG CACCATGTCT GGTTGGCTTT 
GCAGGTCTGT GTAACTCAGC CGATAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
TACACAGTCC ATGGTTTGCA GAGCGGCACC AAATACATCT TCATTGTCAA GGCCATCAAC 
CAGGCGGGCA GCCGCAGCAG TGAGCCTGGG AAGTTGAAGA CAAATAGCCA ACCATTTAAA 
CTGGACCCCA AATCTGCACA TCGAAAGCTA AAAGTGTCAC ATGATAACTT GACAGTAGAA 
CGTGATGAGT CATCATCCAA AAAGAGTCAC ACACCTGAAC GCTTCACCAG CCAAGGGAGC 
TATGGCGTAG CTGGAAACGT GTTCATTGAT AGTGGCCGGC ATTACTGGGA AGTGGTCATA 
AGTGGAAGTA CCTGGTATGC CATTGGCCTT GCTTACAAAT CAGCCCCGAA GCACGAGTGG 
ATTGGGAAGA ACTCTGCTTC CTGGGCACTG TGTCGATGTC ACAATAACTG GGTGGTAAGA 
CACAACAGCA AGGAAATCCC CATTGAGCCA GCCCCCCACC TCCGGCGCGT TGGCATCCTG 
CTAGACTATG ATAACGGCTC CATCGCTTTC TACGATGCTT TGAACTCCAT CCACCTCTAC 
ACCTTTGACA TCACATTCTC GCAGCCTGTG TGCCCCACCT TCACCGTGTG GAACAAATGC 
TTGACCATTA TAACTGGCCT TCCCATCCCA GACCATCTGG ACTGCACAGA GCAACTGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001488161.1
CDS	23..2125
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDERVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTSLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLMEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSEEWVSWGPELRPCKEGISCLRQRTAPCLVGFAGLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCHNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDITFSQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_001488111.4
Organism	Equus caballus(horse)
Definition	Equus caballus midline 1 (MID1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001488111.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCGGA GCTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTCCTGCTAC CTTGTGCACA CAGCCTCTGC TTCAACTGCG CCCATCGCAT CCTGGTGTCA 
CACTGCGCCA CCAACGAGTC CGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGA 
CATGTCATCA CACTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGAAACGT CACCCTGCAG 
AACATCATCG ACAGGTTTCA GAAAGCATCA GTGAGCGGGC CCAATTCCCC CAGCGAGACC 
CGCCGAGAGC GGGCATTCGA CGCCAACACC ATGACCTCCG CCGAGAAAGT CCTCTGCCAA 
TTCTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG CGAAGTGTCC 
TACTGTGATG AGTGCCTGAA AGCCACTCAC CCGAACAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCGCACATC CGGGGGCTGA TGTGCCTGGA GCACGAGGAT 
GAGAGGGTGA ATATGTACTG CGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGAGACCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTGG AGAGTAACCT CACAAGCCTT ATTAAGAGGA ACACGGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAGGTCA ATGCATCGCG TCAAGAAGCC 
AAATTGATGG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAAATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCGGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACCGCTAAGA ATATCACTGA GAGAGTCTCC 
ATGGCAACTG CATCATCCCA GGTCCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACGG CTTCCTATGA CACCATCACC 
GTTCACTGGA CCTCCGATGA TGAATTCAGT GTGGTTTCCT ACGAGCTCCA GTACACCATA 
TTCACTGGAC AAGCCAATGT TGTTAGTGAA GAGTGGGTTT CTTGGGGCCC AGAGCTCAGA 
CCATGTAAGG AGGGAATATC ATGCTTGCGC CAAAGAACTG CACCATGTCT GGTTGGCTTT 
GCAGGTCTGT GTAACTCAGC CGATAGCTGG ATGATCGTGC CCAACATCAA GCAGAACCAC 
TACACAGTCC ATGGTTTGCA GAGCGGCACC AAATACATCT TCATTGTCAA GGCCATCAAC 
CAGGCGGGCA GCCGCAGCAG TGAGCCTGGG AAGTTGAAGA CAAATAGCCA ACCATTTAAA 
CTGGACCCCA AATCTGCACA TCGAAAGCTA AAAGTGTCAC ATGATAACTT GACAGTAGAA 
CGTGATGAGT CATCATCCAA AAAGAGTCAC ACACCTGAAC GCTTCACCAG CCAAGGGAGC 
TATGGCGTAG CTGGAAACGT GTTCATTGAT AGTGGCCGGC ATTACTGGGA AGTGGTCATA 
AGTGGAAGTA CCTGGTATGC CATTGGCCTT GCTTACAAAT CAGCCCCGAA GCACGAGTGG 
ATTGGGAAGA ACTCTGCTTC CTGGGCACTG TGTCGATGTC ACAATAACTG GGTGGTAAGA 
CACAACAGCA AGGAAATCCC CATTGAGCCA GCCCCCCACC TCCGGCGCGT TGGCATCCTG 
CTAGACTATG ATAACGGCTC CATCGCTTTC TACGATGCTT TGAACTCCAT CCACCTCTAC 
ACCTTTGACA TCACATTCTC GCAGCCTGTG TGCCCCACCT TCACCGTGTG GAACAAATGC 
TTGACCATTA TAACTGGCCT TCCCATCCCA GACCATCTGG ACTGCACAGA GCAACTGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MID1 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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