CSF2 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	CSF2
Entrez Gene ID	100033981
Full Name	colony stimulating factor 2
Synonyms	GM-CSF
General protein information	Preferred Names colony stimulating factor 2 Names granulocyte-macrophage colony-stimulating factor
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081882.1	NP_001075351.1	granulocyte-macrophage colony-stimulating factor precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

TRAF6 is required for the GM-CSF-induced JNK, p38 and Akt activation.
Wang Y, Zhou C, Huo J, Ni Y, Zhang P, Lu C, Jing B, Xiao F, Chen W, Li W, Zhang P, Zhang L
Molecular immunology65(2)224-9(2015 Jun)

Annotation of the Protein Coding Regions of the Equine Genome.
Hestand MS, Kalbfleisch TS, Coleman SJ, Zeng Z, Liu J, Orlando L, MacLeod JN
PloS one10(6)e0124375(2015)

Monocyte-derived dendritic cells from horses differ from dendritic cells of humans and mice.
Mauel S, Steinbach F, Ludwig H
Immunology117(4)463-73(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TRAF6 is also required for GM-CSF-induced ubiquitination and activation of Akt.
Title: TRAF6 is required for the GM-CSF-induced JNK, p38 and Akt activation.

The following CSF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb00352	NM_001081882.1 Latest version!	Equus caballus colony stimulating factor 2 (CSF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSF2 ( NM_001081882.1 ) cDNA ORF clone, Equus caballus(horse) -> NP_001075351.1 Equus caballus colony stimulating factor 2 (CSF2), mRNA.

Price & Availability↑

CloneID	OHb00352
Clone ID Related Accession (Same CDS sequence)	NM_001081882.1
Accession Version	NM_001081882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-28
Organism	Equus caballus(horse)
Product	granulocyte-macrophage colony-stimulating factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY040203.2. ##Evidence-Data-START## Transcript exon combination :: AY040203.2, EU438778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2743238, SAMN06854810 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCTTC AGAACCTGCT TCTTCTGGGC ACTGTGGTTT ACAGCATGCC CGCACCCACC 
CGCCAACCCA GCCCTGTCAC TCGGCCCTGG CAGCATGTGG ATGCCATCAA GGAGGCCCTG 
AGCCTTCTGA ACAACAGTAG TGACACTGCT GCTATCATGA ATGAAACAGT AGAAGTCGTC 
TCTGAAACGT TTGACGCCGA GGAGCTGACA TGCCTGCAGA CTCGCCTGAA GCTGTACAAA 
CAGGGCTTGC GGGGCAGCCT CATCAAGCTC GAAGGCCCCT TAACCATGAT GGCCAGCCAC 
TACAAGCAGC ACTGCCCCCC CACCCTGGAA ACTTCCTGTG CAACCCAGAT GATCACCTTC 
AAAAGTTTCA AAAAGAACCT GAAGGATTTT CTGTTTGAGA TCCCGTTTGA CTGCTGGAAC 
CAGCCCAGAA GTAAGGCAGG CCTTCCAGCT AGGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075351.1
CDS	1..459
Translation	MWLQNLLLLGTVVYSMPAPTRQPSPVTRPWQHVDAIKEALSLLNNSSDTAAIMNETVEVVSETFDAEELTCLQTRLKLYKQGLRGSLIKLEGPLTMMASHYKQHCPPTLETSCATQMITFKSFKKNLKDFLFEIPFDCWNQPRSKAGLPARS

Target ORF information:

RefSeq Version	NM_001081882.1
Organism	Equus caballus(horse)
Definition	Equus caballus colony stimulating factor 2 (CSF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081882.1

ORF Insert Sequence:

ATGTGGCTTC AGAACCTGCT TCTTCTGGGC ACTGTGGTTT ACAGCATGCC CGCACCCACC 
CGCCAACCCA GCCCTGTCAC TCGGCCCTGG CAGCATGTGG ATGCCATCAA GGAGGCCCTG 
AGCCTTCTGA ACAACAGTAG TGACACTGCT GCTATCATGA ATGAAACAGT AGAAGTCGTC 
TCTGAAACGT TTGACGCCGA GGAGCTGACA TGCCTGCAGA CTCGCCTGAA GCTGTACAAA 
CAGGGCTTGC GGGGCAGCCT CATCAAGCTC GAAGGCCCCT TAACCATGAT GGCCAGCCAC 
TACAAGCAGC ACTGCCCCCC CACCCTGGAA ACTTCCTGTG CAACCCAGAT GATCACCTTC 
AAAAGTTTCA AAAAGAACCT GAAGGATTTT CTGTTTGAGA TCCCGTTTGA CTGCTGGAAC 
CAGCCCAGAA GTAAGGCAGG CCTTCCAGCT AGGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRAF6 is required for the GM-CSF-induced JNK, p38 and Akt activation.
Molecular immunology65(2)224-9(2015 Jun)
Wang Y,Zhou C,Huo J,Ni Y,Zhang P,Lu C,Jing B,Xiao F,Chen W,Li W,Zhang P,Zhang L

Annotation of the Protein Coding Regions of the Equine Genome.
PloS one10(6)e0124375(2015)
Hestand MS,Kalbfleisch TS,Coleman SJ,Zeng Z,Liu J,Orlando L,MacLeod JN

Monocyte-derived dendritic cells from horses differ from dendritic cells of humans and mice.
Immunology117(4)463-73(2006 Apr)
Mauel S,Steinbach F,Ludwig H