Ir75b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ir75b
Entrez Gene ID	8673994
Full Name	Ionotropic receptor 75b
Synonyms	CG14586,CG14586-B,CG42643,DmelIR75b,Dmel\CG42643,IR75abc,IR75b
General protein information	Preferred Names Ionotropic receptor 75b Names CG42643 gene product from transcript CG42643-RA CG42643-PA Ir75b-PA ionotropic receptor 75b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75A2-75A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144494.2	NP_001137966.2	ionotropic receptor 75b

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ir75a	NP_649012.2
Drosophila melanogaster (fruit fly)	Ir75c	NP_649013.3
Drosophila melanogaster (fruit fly)	Ir75b	NP_001137966.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A single amino acid change in IR75b is sufficient to recode it as a hexanoic acid detector. Expanded representation of this sensory pathway in the brain relies on additional changes in the IR75b promoter and trans-acting loci.
Title: Evolution of Acid-Sensing Olfactory Circuits in Drosophilids.

The following Ir75b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ir75b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19857	NM_001144494.2 Latest version!	Drosophila melanogaster ionotropic receptor 75b (Ir75b), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ir75b ( NM_001144494.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137966.2 Drosophila melanogaster ionotropic receptor 75b (Ir75b), partial mRNA.

Price & Availability↑

CloneID	OFa19857
Clone ID Related Accession (Same CDS sequence)	NM_001144494.2
Accession Version	NM_001144494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ionotropic receptor 75b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_001144494.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTCCAAT TGCACAATTT AATTTTACAC AACCTTATTC ACATGGCTAA ATTGAGTCAT 
GTATTAATTT TGCACTGCTC ACTTTCGCAC CTGGCACTGT TGGCACAGTC TAAAAATATC 
TTCACTCAAT TCCAACCACT ACACTCGGAT ATTCAGCTGA ACGATGACTT TCTTAATCAC 
AATATTCTTA AGCTAGGCGT TTTCTTGGAT ATCAATTGCG ACAAAAGTGG TACAGTTTTG 
GATATGGCCA GTGCTAAACG ATTTTTTAGT CATCGATATC ATTGGTTAAT CTATGATAGG 
TCAATGAACT TTTCCGTACT AGAATCCCAC TTCAAAGAGG CTCAGATTTT CGTTGATGCC 
GATGTTACCT ATGTTACTCA TGATCCTTTC TCTAAAAACT TTTTACTCTA CGATGTATAC 
AACAAGGGGC GACAATTAGG GGGAGAATTA AATATAACTG CCGATCGGGA AATTTTCTGC 
AATAAAACGA ACTGTCGAGT TGAACGATAT CTGAGTGAAC TTTACACACG GAGTGCTTTG 
CAGCATAGAA AGTCATTTAC TGGATTAACC ATGAGAGCAA CAGCTGTGGT GACCGCTTTA 
CCACTTAATG TCTCCATAAA GGAAATCTTT GACTTTATGA ACTCCAAATA TCGGATTCAA 
TTAGATACCT ACGCTAGATT GGGTTACCAG GCGCGTCAGC CACTGAGGGA TATGTTGGAC 
TGCAAGTTTA AGTACATATT TAGAGATCGG TGGTCGGATG GCAATGCAAC AGGCGGCATG 
ATTGGAGATC TCATCCTGGA TAAAGCTGAC CTGGCGATAG CTCCTTTCAT TTATTCCTTC 
GATCGCGCTT TGTTTCTGCA ACCCATCACG AAATTTAGTG TTTTCCGTGA AATCTGCATG 
TTCCGTAATC CCCGATCCGT TTCAGCGGGT CTCAGTGCCA CGGAGTTCCT GCAGCCCTTC 
AGCGGAGGAG TTTGGTTGAC ATTTGCACTT TTACTCCTCC TGGCAGGCTG TCTCCTTTGG 
GTCACCTTCA TCTTGGAGCG CCGTAAGCAG TGGAAGCCAT CGCTACTCAC CAGCTGCCTT 
CTTTCCTTCG GAGCTGGTTG CATTCAAGGG GCGTGGCTTA CGCCAAGATC AATGGGTGGG 
AGAATGGCCT TCTTTGCCCT AATGGTGACC TCATATTTGA TGTACAACTA CTACACATCC 
ATTGTGGTGT CCAAGCTACT GGGCCAGCCG ATTAAGTCCA ACATACGTAC ACTGCAACAA 
CTGGCAGATA GCAATTTGGA TGTGGGCATA GAGCCAACTG TTTATACCCG CATCTATGTG 
GAAACCTCCG AGGAGCCGGA TGTGCGTGAC CTCTACCGCA AAAAAGTGCT TGGCAGCAAG 
CGATCCCCGG ATAAAATATG GATTCCCACA GAGGCTGGGG TTCTCTCTGT AAGGGACCAG 
GAAGGCTTCG TCTACATAAC TGGCGTGGCA ACGGGCTACG AGTTCGTGAG GAAGCACTTC 
CTGGCCCACC AGATCTGCGA GCTCAACGAG ATTCCTCTAC GCGATGCCTC CCACACCCAC 
ACAGTCCTCG CGAAGAGGTC ACCCTATGCC GAACTCATCA AACTAAGTGA GCTGCGTATG 
CTGGAGACAG GAGTTCATTT CAAGCACGAG CGGTCTTGGA TGGAGACAAA ACTCCACTGC 
TACCAGCATA ATCACACGGT GGCCGTGGGT CTGGAGTACG CTGCTCCGCT GTTCATCATC 
CTATTGGGAG CGATAATTCT CTGCATGGGC ATATTGGGAC TGGAGGTCAT CTGGCACCGC 
CACTGCACTC TGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137966.2
CDS	1..1818
Translation	MLQLHNLILHNLIHMAKLSHVLILHCSLSHLALLAQSKNIFTQFQPLHSDIQLNDDFLNHNILKLGVFLDINCDKSGTVLDMASAKRFFSHRYHWLIYDRSMNFSVLESHFKEAQIFVDADVTYVTHDPFSKNFLLYDVYNKGRQLGGELNITADREIFCNKTNCRVERYLSELYTRSALQHRKSFTGLTMRATAVVTALPLNVSIKEIFDFMNSKYRIQLDTYARLGYQARQPLRDMLDCKFKYIFRDRWSDGNATGGMIGDLILDKADLAIAPFIYSFDRALFLQPITKFSVFREICMFRNPRSVSAGLSATEFLQPFSGGVWLTFALLLLLAGCLLWVTFILERRKQWKPSLLTSCLLSFGAGCIQGAWLTPRSMGGRMAFFALMVTSYLMYNYYTSIVVSKLLGQPIKSNIRTLQQLADSNLDVGIEPTVYTRIYVETSEEPDVRDLYRKKVLGSKRSPDKIWIPTEAGVLSVRDQEGFVYITGVATGYEFVRKHFLAHQICELNEIPLRDASHTHTVLAKRSPYAELIKLSELRMLETGVHFKHERSWMETKLHCYQHNHTVAVGLEYAAPLFIILLGAIILCMGILGLEVIWHRHCTLH

Target ORF information:

RefSeq Version	NM_001144494.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ionotropic receptor 75b (Ir75b), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144494.2

ORF Insert Sequence:

ATGCTCCAAT TGCACAATTT AATTTTACAC AACCTTATTC ACATGGCTAA ATTGAGTCAT 
GTATTAATTT TGCACTGCTC ACTTTCGCAC CTGGCACTGT TGGCACAGTC TAAAAATATC 
TTCACTCAAT TCCAACCACT ACACTCGGAT ATTCAGCTGA ACGATGACTT TCTTAATCAC 
AATATTCTTA AGCTAGGCGT TTTCTTGGAT ATCAATTGCG ACAAAAGTGG TACAGTTTTG 
GATATGGCCA GTGCTAAACG ATTTTTTAGT CATCGATATC ATTGGTTAAT CTATGATAGG 
TCAATGAACT TTTCCGTACT AGAATCCCAC TTCAAAGAGG CTCAGATTTT CGTTGATGCC 
GATGTTACCT ATGTTACTCA TGATCCTTTC TCTAAAAACT TTTTACTCTA CGATGTATAC 
AACAAGGGGC GACAATTAGG GGGAGAATTA AATATAACTG CCGATCGGGA AATTTTCTGC 
AATAAAACGA ACTGTCGAGT TGAACGATAT CTGAGTGAAC TTTACACACG GAGTGCTTTG 
CAGCATAGAA AGTCATTTAC TGGATTAACC ATGAGAGCAA CAGCTGTGGT GACCGCTTTA 
CCACTTAATG TCTCCATAAA GGAAATCTTT GACTTTATGA ACTCCAAATA TCGGATTCAA 
TTAGATACCT ACGCTAGATT GGGTTACCAG GCGCGTCAGC CACTGAGGGA TATGTTGGAC 
TGCAAGTTTA AGTACATATT TAGAGATCGG TGGTCGGATG GCAATGCAAC AGGCGGCATG 
ATTGGAGATC TCATCCTGGA TAAAGCTGAC CTGGCGATAG CTCCTTTCAT TTATTCCTTC 
GATCGCGCTT TGTTTCTGCA ACCCATCACG AAATTTAGTG TTTTCCGTGA AATCTGCATG 
TTCCGTAATC CCCGATCCGT TTCAGCGGGT CTCAGTGCCA CGGAGTTCCT GCAGCCCTTC 
AGCGGAGGAG TTTGGTTGAC ATTTGCACTT TTACTCCTCC TGGCAGGCTG TCTCCTTTGG 
GTCACCTTCA TCTTGGAGCG CCGTAAGCAG TGGAAGCCAT CGCTACTCAC CAGCTGCCTT 
CTTTCCTTCG GAGCTGGTTG CATTCAAGGG GCGTGGCTTA CGCCAAGATC AATGGGTGGG 
AGAATGGCCT TCTTTGCCCT AATGGTGACC TCATATTTGA TGTACAACTA CTACACATCC 
ATTGTGGTGT CCAAGCTACT GGGCCAGCCG ATTAAGTCCA ACATACGTAC ACTGCAACAA 
CTGGCAGATA GCAATTTGGA TGTGGGCATA GAGCCAACTG TTTATACCCG CATCTATGTG 
GAAACCTCCG AGGAGCCGGA TGTGCGTGAC CTCTACCGCA AAAAAGTGCT TGGCAGCAAG 
CGATCCCCGG ATAAAATATG GATTCCCACA GAGGCTGGGG TTCTCTCTGT AAGGGACCAG 
GAAGGCTTCG TCTACATAAC TGGCGTGGCA ACGGGCTACG AGTTCGTGAG GAAGCACTTC 
CTGGCCCACC AGATCTGCGA GCTCAACGAG ATTCCTCTAC GCGATGCCTC CCACACCCAC 
ACAGTCCTCG CGAAGAGGTC ACCCTATGCC GAACTCATCA AACTAAGTGA GCTGCGTATG 
CTGGAGACAG GAGTTCATTT CAAGCACGAG CGGTCTTGGA TGGAGACAAA ACTCCACTGC 
TACCAGCATA ATCACACGGT GGCCGTGGGT CTGGAGTACG CTGCTCCGCT GTTCATCATC 
CTATTGGGAG CGATAATTCT CTGCATGGGC ATATTGGGAC TGGAGGTCAT CTGGCACCGC 
CACTGCACTC TGCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ir75b cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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