Apl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apl
Entrez Gene ID	59216
Full Name	CG32165 gene product from transcript CG32165-RC
Synonyms	CG18218,CG18861,Dmel\CG32165,Imp4/5,anon-73Bb
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 73A11-73B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_168676.2	NP_730182.1	apollo, isoform A
NM_001275027.1	NP_001261956.1	apollo, isoform B
NM_001300155.1	NP_001287084.1	apollo, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IPO4	XP_001168091.1
Bos taurus (cattle)	IPO4	NP_001077130.1
Danio rerio (zebrafish)	ipo4	XP_684163.4
Drosophila melanogaster (fruit fly)	CG32165	NP_730182.1
Saccharomyces cerevisiae (baker's yeast)	KAP123	NP_011035.1
Eremothecium gossypii	AGOS_AGL298C	NP_986369.2
Homo sapiens (human)	IPO4	NP_078934.3
Mus musculus (house mouse)	Ipo4	NP_077229.4
Schizosaccharomyces pombe (fission yeast)	kap123	NP_596729.1
	IPO4	XP_002808502.1
Canis lupus familiaris (dog)	IPO4	XP_537384.3
Drosophila melanogaster (fruit fly)	CG32164	NP_001261957.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006942	XP_308814.4
Kluyveromyces lactis	KLLA0E20769g	XP_454889.1
Magnaporthe oryzae (rice blast fungus)	MGG_16711	XP_003711698.1
Arabidopsis thaliana (thale cress)	AT4G27640	NP_194494.2
Xenopus tropicalis (tropical clawed frog)	ipo4	NP_001008011.2
Rattus norvegicus (Norway rat)	Ipo4	NP_001099508.1
Neurospora crassa	NCU05650	XP_960211.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Apl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19480	NM_168676.2 Latest version!	Drosophila melanogaster apollo, transcript variant A (Apl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20413	NM_001275027.1 Latest version!	Drosophila melanogaster apollo, transcript variant B (Apl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20413	NM_001300155.1 Latest version!	Drosophila melanogaster apollo, transcript variant C (Apl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apl ( NM_168676.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730182.1 Drosophila melanogaster apollo, transcript variant A (Apl), mRNA.

Price & Availability↑

CloneID	OFa19480
Clone ID Related Accession (Same CDS sequence)	NM_168676.2
Accession Version	NM_168676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apollo, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168676.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCAG CTATTCTGGA TATAATCAAC GGGATCCTGA CCATAGACAC GGAACGAATC 
CGTGAGTCCA CAGCCAAGAT GCTAAAGGCC TACGAGAATC CCGATTCTCT GCTGGTTCTC 
ACCCAGATCG TGATGTCGGA CAGGCCGGTC CAAGAGCGGC AGGTCGCCGC CGTGCTCCTC 
AAGAGGCGGG TCAAAAAGTT ACGCCACTGG CAGTTGGTTC CCGCCGAGCA CCAGGCAGCA 
ATCAAATCGA ACATGCTACA GGTCCTCATC GCGGTGAAGG AAAAGACCGT GAAGGGCACG 
GTGGCCTTTA TAATTGGATC GCTGGTGCGC CACGAGGAGG GCGAGCAAAA CTCCTGGAGG 
GAGGAAATAC TGAAGTTTAT TTACGAACGT TGCAGCAGCC CCGATCCAAT AGAAAGCGAG 
CGGGGCAGCT CAATCTTTTC CTCGCTCATG GACGCTGCTC CCGATCAGTT TTCGGACCAC 
ACGGATACTA TGTTCCCATT GCTTGCCGGC ATTCTAGTTA CCGCCGAGGC GAATGGGAAC 
ATGGCCACAC CCACCGTGCA TAACATGCTG ACCGGTTCAT GCTTCTTGTT GCCTTTCGTA 
AGTGGACATA GTAATGCTGA GCAGATTGTG GTTAAGGCTG TGCCGCTCAT ACTCAAGGCC 
CTGGCCGCCT TTGTCGAAAA GGGGTACAGT ATTGAGTTCA TGGGTGCCTT TGATATCATC 
GACAGCATGG CCGAGCATGT GCCCCACTTG CTGACCGGTA ATGTGAAGCT GCTTCTGGAG 
TTTTGTCTGA TGATTGCGAG AAACAAGCAG TTCGACGCTT CGATTCGGGT TCAGGTGCTT 
ACCTTTGTAG GCAGCCTAGT TCGCCTCAAG AAGAAAATTA TTATGAAGCA GAAACTACTA 
CAGCCCACAC TATCTGTTCT TTTCGAAGTG ATTTGCCAGG ACGACCTCGA GGAAGGTGAC 
GATGATTACT TTTCCTCGGA GAGCCTGAGT AGTCCATCCA ATGCAGCTGC GCAGACACTG 
GACCTGATGG CCCTTCACAT GGTGCCGGAC AAGTTTATTC CGCCACTACT GGATTTGCTG 
GAGCCTGCGT TGCAGAGCCC GGAACCTGTG CTTCGCCGTT CCTCTTTTAT TTGCATGGGC 
GTTATTGCCG AGGGCTGTTC CGAGGCCATT GGGAAAAAGT ATCTCGAGGT CATGCTAAAT 
ATCATCAAAG CTGGAGTGTT GGACTCTGTT ATGTTCGTGC GGACCGCCGC ATTCTTCGCC 
CTCGGTCAGT TTTCCGAGTT TCTTCAGCCG ACGATCTGCA AGTTTGCTCC CCAAATTCTA 
CCCGTGTTGT TCGACTATCT AAACCAGCTG GTACTGGAAC TCAAGGTTGG TGAGCCGGAT 
TCGAAACATA TGGATCGTAT GTTTTATGCC CTGGAGACCT TTTGCGAGAA TCTGGATGAG 
GATATTGTTC CTTATTTACC CACACTTATG GATCGTCTGT TTGGGGTTAT GGAACCGCAG 
AACTCAAATC AAATGCGTGA AATGGCTTTG TCGGCCATAG CAGCGGTGTC AGCAGCAGCT 
AAGGAAAACC TGATGCCATA TTTCCCCAGG ATCATGACCG TACTACAGGG CTGTCTGGTG 
AAGGATTGCC CAAAAGAAAT GTACAGTCAG CGCATCCAAG CTATCGACAC TCTGGCCGCG 
CTGTGTCGCG AATTGGGCAA GGATAATATT ATTCCTCTTG CCGACGATAC AATGAACTTC 
TGCCTGATGA TGCTGGAAGA CGGTCCGGAT GATCCCGAGT ACCGCAGGAG TATATACAAC 
CTGATGTCCT CCCTGTCATC AGTTGTCAAC GAGAGCATGG CCAGTGTGTT CCCCAAGTTC 
ATTGATCGCA TAATGGAGTC TGTGATTTTT TCGGAGGACA TGGTGCCAAA TGTGTCGGAC 
AATGCGGACG ATGATTTGGC CCTAGTAGAT GCCCCCGACA TAGAGATTGA TTTAGAGCAC 
ACAGACGACG AAGATGACCA AGACGCTTAC CCGGTGGAGA ATGATTATAT CGTCGAAAAG 
GAGGAGGCCA TTCTGTCACT CAAGGAGTTT GCTACCCATA CCGGCGCCGC CTTCGCGCCC 
TATTTGCAAT CTGCATTCGA GAACGTCTAC AAGATGATTG ATCATCCGCA AGGCGACGTT 
CGTATGGCAT GTATTGACTC CATTTGCTCG TTCATTACGG CTCTCCACAA GTTGGATGAT 
GCCGCCGGCC TGAAGCGCGC CTGCGAGATT GCCATTCCAA AGTTTGCACA TATAATGCGC 
ACCGACGACC AGGTTGCGGT CGTACTCCGC ATGCTTGACG TCCTCTACGA TGTCTTCAAG 
TACGTGCCGG CGATAAACAG CCAGGAGCAC GCCGAACTTA TATTCGGTTG CATCAGGGAC 
ATCTTCACAA ATAAAATGGC CTGTCAGTTT AACGAGGAGA GCGGCGGCGG AGATGATGAG 
TGTTCGGAGG AAAGCGAGAA CGACGAGATG CTGTTCGAGA ACGCTGCCAA TCTGTTCCCC 
ATGTTTGGCT TAACCCTTCA GCCGGAGCTG TTCTCGCTTT ATTTTGGACG CCTTTACCAT 
TTCTATATTC AAAGGCTGGC GAAGGTAAAG GAGCGCGATT TACCAGAACA GCGGGCATAC 
ATCTATGGCG CACTTGCTGA CTGCTGCAAG GCGTTAAAAG GTTGCTGTGC TACTTATTTC 
GATGCCCTAC GTCCCATTTT TATCGCCGGT TCCAGGGATT CTGATGCCAA AGCGCGACAG 
AACTCCTATT TTGCACTAGG CGAAATTGTC TTCCATTCCG AGGAAAAATC ATTCGAGTCT 
TATCCGACAA TTTTGCAAGC CCTTTCCGAA GCAATTGTCA GGGAATCCGT TCCTGCCGCC 
ATGGACAACA TTTGCGGAGC AGTTGCTCGT CTCATAGTTA CCAACCCAGA TTCAGTGCCG 
CTTGGCCAGG TGCTGCCCGT GTGGCTCAAC CATTTGCCAC TGAAGGATGA CACCGTGGAG 
AACGATGTGA TTCAGAAGGC GTTCCGTGTG CTATACTTGA AGGCCCGCCC CAGCATTGAG 
GCACATCTTG AGCAAATCCT GGCCATCACC ATAGAGGCGA GTTACAAGAG ACAGATGCCT 
GACGTCGAGA CAACCGAGAG CGCGGTGGCC CTCATCAAAG AGATTCGCGC CAACTACCCG 
GAACTGTTCA GCAAAGTATC GAACATGAAT CCAGAAGTGT TCAATTATGT GCAAGCCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730182.1
CDS	115..3357
Translation	MEAAILDIINGILTIDTERIRESTAKMLKAYENPDSLLVLTQIVMSDRPVQERQVAAVLLKRRVKKLRHWQLVPAEHQAAIKSNMLQVLIAVKEKTVKGTVAFIIGSLVRHEEGEQNSWREEILKFIYERCSSPDPIESERGSSIFSSLMDAAPDQFSDHTDTMFPLLAGILVTAEANGNMATPTVHNMLTGSCFLLPFVSGHSNAEQIVVKAVPLILKALAAFVEKGYSIEFMGAFDIIDSMAEHVPHLLTGNVKLLLEFCLMIARNKQFDASIRVQVLTFVGSLVRLKKKIIMKQKLLQPTLSVLFEVICQDDLEEGDDDYFSSESLSSPSNAAAQTLDLMALHMVPDKFIPPLLDLLEPALQSPEPVLRRSSFICMGVIAEGCSEAIGKKYLEVMLNIIKAGVLDSVMFVRTAAFFALGQFSEFLQPTICKFAPQILPVLFDYLNQLVLELKVGEPDSKHMDRMFYALETFCENLDEDIVPYLPTLMDRLFGVMEPQNSNQMREMALSAIAAVSAAAKENLMPYFPRIMTVLQGCLVKDCPKEMYSQRIQAIDTLAALCRELGKDNIIPLADDTMNFCLMMLEDGPDDPEYRRSIYNLMSSLSSVVNESMASVFPKFIDRIMESVIFSEDMVPNVSDNADDDLALVDAPDIEIDLEHTDDEDDQDAYPVENDYIVEKEEAILSLKEFATHTGAAFAPYLQSAFENVYKMIDHPQGDVRMACIDSICSFITALHKLDDAAGLKRACEIAIPKFAHIMRTDDQVAVVLRMLDVLYDVFKYVPAINSQEHAELIFGCIRDIFTNKMACQFNEESGGGDDECSEESENDEMLFENAANLFPMFGLTLQPELFSLYFGRLYHFYIQRLAKVKERDLPEQRAYIYGALADCCKALKGCCATYFDALRPIFIAGSRDSDAKARQNSYFALGEIVFHSEEKSFESYPTILQALSEAIVRESVPAAMDNICGAVARLIVTNPDSVPLGQVLPVWLNHLPLKDDTVENDVIQKAFRVLYLKARPSIEAHLEQILAITIEASYKRQMPDVETTESAVALIKEIRANYPELFSKVSNMNPEVFNYVQAL

Target ORF information:

RefSeq Version	NM_168676.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apollo, transcript variant A (Apl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168676.2

ORF Insert Sequence:

ATGGAGGCAG CTATTCTGGA TATAATCAAC GGGATCCTGA CCATAGACAC GGAACGAATC 
CGTGAGTCCA CAGCCAAGAT GCTAAAGGCC TACGAGAATC CCGATTCTCT GCTGGTTCTC 
ACCCAGATCG TGATGTCGGA CAGGCCGGTC CAAGAGCGGC AGGTCGCCGC CGTGCTCCTC 
AAGAGGCGGG TCAAAAAGTT ACGCCACTGG CAGTTGGTTC CCGCCGAGCA CCAGGCAGCA 
ATCAAATCGA ACATGCTACA GGTCCTCATC GCGGTGAAGG AAAAGACCGT GAAGGGCACG 
GTGGCCTTTA TAATTGGATC GCTGGTGCGC CACGAGGAGG GCGAGCAAAA CTCCTGGAGG 
GAGGAAATAC TGAAGTTTAT TTACGAACGT TGCAGCAGCC CCGATCCAAT AGAAAGCGAG 
CGGGGCAGCT CAATCTTTTC CTCGCTCATG GACGCTGCTC CCGATCAGTT TTCGGACCAC 
ACGGATACTA TGTTCCCATT GCTTGCCGGC ATTCTAGTTA CCGCCGAGGC GAATGGGAAC 
ATGGCCACAC CCACCGTGCA TAACATGCTG ACCGGTTCAT GCTTCTTGTT GCCTTTCGTA 
AGTGGACATA GTAATGCTGA GCAGATTGTG GTTAAGGCTG TGCCGCTCAT ACTCAAGGCC 
CTGGCCGCCT TTGTCGAAAA GGGGTACAGT ATTGAGTTCA TGGGTGCCTT TGATATCATC 
GACAGCATGG CCGAGCATGT GCCCCACTTG CTGACCGGTA ATGTGAAGCT GCTTCTGGAG 
TTTTGTCTGA TGATTGCGAG AAACAAGCAG TTCGACGCTT CGATTCGGGT TCAGGTGCTT 
ACCTTTGTAG GCAGCCTAGT TCGCCTCAAG AAGAAAATTA TTATGAAGCA GAAACTACTA 
CAGCCCACAC TATCTGTTCT TTTCGAAGTG ATTTGCCAGG ACGACCTCGA GGAAGGTGAC 
GATGATTACT TTTCCTCGGA GAGCCTGAGT AGTCCATCCA ATGCAGCTGC GCAGACACTG 
GACCTGATGG CCCTTCACAT GGTGCCGGAC AAGTTTATTC CGCCACTACT GGATTTGCTG 
GAGCCTGCGT TGCAGAGCCC GGAACCTGTG CTTCGCCGTT CCTCTTTTAT TTGCATGGGC 
GTTATTGCCG AGGGCTGTTC CGAGGCCATT GGGAAAAAGT ATCTCGAGGT CATGCTAAAT 
ATCATCAAAG CTGGAGTGTT GGACTCTGTT ATGTTCGTGC GGACCGCCGC ATTCTTCGCC 
CTCGGTCAGT TTTCCGAGTT TCTTCAGCCG ACGATCTGCA AGTTTGCTCC CCAAATTCTA 
CCCGTGTTGT TCGACTATCT AAACCAGCTG GTACTGGAAC TCAAGGTTGG TGAGCCGGAT 
TCGAAACATA TGGATCGTAT GTTTTATGCC CTGGAGACCT TTTGCGAGAA TCTGGATGAG 
GATATTGTTC CTTATTTACC CACACTTATG GATCGTCTGT TTGGGGTTAT GGAACCGCAG 
AACTCAAATC AAATGCGTGA AATGGCTTTG TCGGCCATAG CAGCGGTGTC AGCAGCAGCT 
AAGGAAAACC TGATGCCATA TTTCCCCAGG ATCATGACCG TACTACAGGG CTGTCTGGTG 
AAGGATTGCC CAAAAGAAAT GTACAGTCAG CGCATCCAAG CTATCGACAC TCTGGCCGCG 
CTGTGTCGCG AATTGGGCAA GGATAATATT ATTCCTCTTG CCGACGATAC AATGAACTTC 
TGCCTGATGA TGCTGGAAGA CGGTCCGGAT GATCCCGAGT ACCGCAGGAG TATATACAAC 
CTGATGTCCT CCCTGTCATC AGTTGTCAAC GAGAGCATGG CCAGTGTGTT CCCCAAGTTC 
ATTGATCGCA TAATGGAGTC TGTGATTTTT TCGGAGGACA TGGTGCCAAA TGTGTCGGAC 
AATGCGGACG ATGATTTGGC CCTAGTAGAT GCCCCCGACA TAGAGATTGA TTTAGAGCAC 
ACAGACGACG AAGATGACCA AGACGCTTAC CCGGTGGAGA ATGATTATAT CGTCGAAAAG 
GAGGAGGCCA TTCTGTCACT CAAGGAGTTT GCTACCCATA CCGGCGCCGC CTTCGCGCCC 
TATTTGCAAT CTGCATTCGA GAACGTCTAC AAGATGATTG ATCATCCGCA AGGCGACGTT 
CGTATGGCAT GTATTGACTC CATTTGCTCG TTCATTACGG CTCTCCACAA GTTGGATGAT 
GCCGCCGGCC TGAAGCGCGC CTGCGAGATT GCCATTCCAA AGTTTGCACA TATAATGCGC 
ACCGACGACC AGGTTGCGGT CGTACTCCGC ATGCTTGACG TCCTCTACGA TGTCTTCAAG 
TACGTGCCGG CGATAAACAG CCAGGAGCAC GCCGAACTTA TATTCGGTTG CATCAGGGAC 
ATCTTCACAA ATAAAATGGC CTGTCAGTTT AACGAGGAGA GCGGCGGCGG AGATGATGAG 
TGTTCGGAGG AAAGCGAGAA CGACGAGATG CTGTTCGAGA ACGCTGCCAA TCTGTTCCCC 
ATGTTTGGCT TAACCCTTCA GCCGGAGCTG TTCTCGCTTT ATTTTGGACG CCTTTACCAT 
TTCTATATTC AAAGGCTGGC GAAGGTAAAG GAGCGCGATT TACCAGAACA GCGGGCATAC 
ATCTATGGCG CACTTGCTGA CTGCTGCAAG GCGTTAAAAG GTTGCTGTGC TACTTATTTC 
GATGCCCTAC GTCCCATTTT TATCGCCGGT TCCAGGGATT CTGATGCCAA AGCGCGACAG 
AACTCCTATT TTGCACTAGG CGAAATTGTC TTCCATTCCG AGGAAAAATC ATTCGAGTCT 
TATCCGACAA TTTTGCAAGC CCTTTCCGAA GCAATTGTCA GGGAATCCGT TCCTGCCGCC 
ATGGACAACA TTTGCGGAGC AGTTGCTCGT CTCATAGTTA CCAACCCAGA TTCAGTGCCG 
CTTGGCCAGG TGCTGCCCGT GTGGCTCAAC CATTTGCCAC TGAAGGATGA CACCGTGGAG 
AACGATGTGA TTCAGAAGGC GTTCCGTGTG CTATACTTGA AGGCCCGCCC CAGCATTGAG 
GCACATCTTG AGCAAATCCT GGCCATCACC ATAGAGGCGA GTTACAAGAG ACAGATGCCT 
GACGTCGAGA CAACCGAGAG CGCGGTGGCC CTCATCAAAG AGATTCGCGC CAACTACCCG 
GAACTGTTCA GCAAAGTATC GAACATGAAT CCAGAAGTGT TCAATTATGT GCAAGCCCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apl ( NM_001275027.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261956.1 Drosophila melanogaster apollo, transcript variant B (Apl), mRNA.

Price & Availability↑

CloneID	OFa20413
Clone ID Related Accession (Same CDS sequence)	NM_001275027.1 , NM_001300155.1
Accession Version	NM_001275027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apollo, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCAG CTATTCTGGA TATAATCAAC GGGATCCTGA CCATAGACAC GGAACGAATC 
CGTGAGTCCA CAGCCAAGAT GCTAAAGGCC TACGAGAATC CCGATTCTCT GCTGGTTCTC 
ACCCAGATCG TGATGTCGGA CAGGCCGGTC CAAGAGCGGC AGGTCGCCGC CGTGCTCCTC 
AAGAGGCGGG TCAAAAAGTT ACGCCACTGG CAGTTGGTTC CCGCCGAGCA CCAGGCAGCA 
ATCAAATCGA ACATGCTACA GGTCCTCATC GCGGTGAAGG AAAAGACCGT GAAGGGCACG 
GTGGCCTTTA TAATTGGATC GCTGGTGCGC CACGAGGAGG GCGAGCAAAA CTCCTGGAGG 
GAGGAAATAC TGAAGTTTAT TTACGAACGT TGCAGCAGCC CCGATCCAAT AGAAAGCGAG 
CGGGGCAGCT CAATCTTTTC CTCGCTCATG GACGCTGCTC CCGATCAGTT TTCGGACCAC 
ACGGATACTA TGTTCCCATT GCTTGCCGGC ATTCTAGTTA CCGCCGAGGC GAATGGGAAC 
ATGGCCACAC CCACCGTGCA TAACATGCTG ACCGGTTCAT GCTTCTTGTT GCCTTTCGCC 
CTGGCCGCCT TTGTCGAAAA GGGGTACAGT ATTGAGTTCA TGGGTGCCTT TGATATCATC 
GACAGCATGG CCGAGCATGT GCCCCACTTG CTGACCGGTA ATGTGAAGCT GCTTCTGGAG 
TTTTGTCTGA TGATTGCGAG AAACAAGCAG TTCGACGCTT CGATTCGGGT TCAGGTGCTT 
ACCTTTGTAG GCAGCCTAGT TCGCCTCAAG AAGAAAATTA TTATGAAGCA GAAACTACTA 
CAGCCCACAC TATCTGTTCT TTTCGAAGTG ATTTGCCAGG ACGACCTCGA GGAAGACGAT 
GATTACTTTT CCTCGGAGAG CCTGAGTAGT CCATCCAATG CAGCTGCGCA GACACTGGAC 
CTGATGGCCC TTCACATGGT GCCGGACAAG TTTATTCCGC CACTACTGGA TTTGCTGGAG 
CCTGCGTTGC AGAGCCCGGA ACCTGTGCTT CGCCGTTCCT CTTTTATTTG CATGGGCGTT 
ATTGCCGAGG GCTGTTCCGA GGCCATTGGG AAAAAGTATC TCGAGGTCAT GCTAAATATC 
ATCAAAGCTG GAGTGTTGGA CTCTGTTATG TTCGTGCGGA CCGCCGCATT CTTCGCCCTC 
GGTCAGTTTT CCGAGTTTCT TCAGCCGACG ATCTGCAAGT TTGCTCCCCA AATTCTACCC 
GTGTTGTTCG ACTATCTAAA CCAGCTGGTA CTGGAACTCA AGGTTGGTGA GCCGGATTCG 
AAACATATGG ATCGTATGTT TTATGCCCTG GAGACCTTTT GCGAGAATCT GGATGAGGAT 
ATTGTTCCTT ATTTACCCAC ACTTATGGAT CGTCTGTTTG GGGTTATGGA ACCGCAGAAC 
TCAAATCAAA TGCGTGAAAT GGCTTTGTCG GCCATAGCAG CGGTGTCAGC AGCAGCTAAG 
GAAAACCTGA TGCCATATTT CCCCAGGATC ATGACCGTAC TACAGGGCTG TCTGGTGAAG 
GATTGCCCAA AAGAAATGTA CAGTCAGCGC ATCCAAGCTA TCGACACTCT GGCCGCGCTG 
TGTCGCGAAT TGGGCAAGGA TAATATTATT CCTCTTGCCG ACGATACAAT GAACTTCTGC 
CTGATGATGC TGGAAGACGG TCCGGATGAT CCCGAGTACC GCAGGAGTAT ATACAACCTG 
ATGTCCTCCC TGTCATCAGT TGTCAACGAG AGCATGGCCA GTGTGTTCCC CAAGTTCATT 
GATCGCATAA TGGAGTCTGT GATTTTTTCG GAGGACATGG TGCCAAATGT GTCGGACAAT 
GCGGACGATG ATTTGGCCCT AGTAGATGCC CCCGACATAG AGATTGATTT AGAGCACACA 
GACGACGAAG ATGACCAAGA CGCTTACCCG GTGGAGAATG ATTATATCGT CGAAAAGGAG 
GAGGCCATTC TGTCACTCAA GGAGTTTGCT ACCCATACCG GCGCCGCCTT CGCGCCCTAT 
TTGCAATCTG CATTCGAGAA CGTCTACAAG ATGATTGATC ATCCGCAAGG CGACGTTCGT 
ATGGCATGTA TTGACTCCAT TTGCTCGTTC ATTACGGCTC TCCACAAGTT GGATGATGCC 
GCCGGCCTGA AGCGCGCCTG CGAGATTGCC ATTCCAAAGT TTGCACATAT AATGCGCACC 
GACGACCAGG TTGCGGTCGT ACTCCGCATG CTTGACGTCC TCTACGATGT CTTCAAGTAC 
GTGCCGGCGA TAAACAGCCA GGAGCACGCC GAACTTATAT TCGGTTGCAT CAGGGACATC 
TTCACAAATA AAATGGCCTG TCAGTTTAAC GAGGAGAGCG GCGGCGGAGA TGATGAGTGT 
TCGGAGGAAA GCGAGAACGA CGAGATGCTG TTCGAGAACG CTGCCAATCT GTTCCCCATG 
TTTGGCTTAA CCCTTCAGCC GGAGCTGTTC TCGCTTTATT TTGGACGCCT TTACCATTTC 
TATATTCAAA GGCTGGCGAA GGTAAAGGAG CGCGATTTAC CAGAACAGCG GGCATACATC 
TATGGCGCAC TTGCTGACTG CTGCAAGGCG TTAAAAGGTT GCTGTGCTAC TTATTTCGAT 
GCCCTACGTC CCATTTTTAT CGCCGGTTCC AGGGATTCTG ATGCCAAAGC GCGACAGAAC 
TCCTATTTTG CACTAGGCGA AATTGTCTTC CATTCCGAGG AAAAATCATT CGAGTCTTAT 
CCGACAATTT TGCAAGCCCT TTCCGAAGCA ATTGTCAGGG AATCCGTTCC TGCCGCCATG 
GACAACATTT GCGGAGCAGT TGCTCGTCTC ATAGTTACCA ACCCAGATTC AGTGCCGCTT 
GGCCAGGTGC TGCCCGTGTG GCTCAACCAT TTGCCACTGA AGGATGACAC CGTGGAGAAC 
GATGTGATTC AGAAGGCGTT CCGTGTGCTA TACTTGAAGG CCCGCCCCAG CATTGAGGCA 
CATCTTGAGC AAATCCTGGC CATCACCATA GAGGCGAGTT ACAAGAGACA GATGCCTGAC 
GTCGAGACAA CCGAGAGCGC GGTGGCCCTC ATCAAAGAGA TTCGCGCCAA CTACCCGGAA 
CTGTTCAGCA AAGTATCGAA CATGAATCCA GAAGTGTTCA ATTATGTGCA AGCCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261956.1
CDS	115..3294
Translation	MEAAILDIINGILTIDTERIRESTAKMLKAYENPDSLLVLTQIVMSDRPVQERQVAAVLLKRRVKKLRHWQLVPAEHQAAIKSNMLQVLIAVKEKTVKGTVAFIIGSLVRHEEGEQNSWREEILKFIYERCSSPDPIESERGSSIFSSLMDAAPDQFSDHTDTMFPLLAGILVTAEANGNMATPTVHNMLTGSCFLLPFALAAFVEKGYSIEFMGAFDIIDSMAEHVPHLLTGNVKLLLEFCLMIARNKQFDASIRVQVLTFVGSLVRLKKKIIMKQKLLQPTLSVLFEVICQDDLEEDDDYFSSESLSSPSNAAAQTLDLMALHMVPDKFIPPLLDLLEPALQSPEPVLRRSSFICMGVIAEGCSEAIGKKYLEVMLNIIKAGVLDSVMFVRTAAFFALGQFSEFLQPTICKFAPQILPVLFDYLNQLVLELKVGEPDSKHMDRMFYALETFCENLDEDIVPYLPTLMDRLFGVMEPQNSNQMREMALSAIAAVSAAAKENLMPYFPRIMTVLQGCLVKDCPKEMYSQRIQAIDTLAALCRELGKDNIIPLADDTMNFCLMMLEDGPDDPEYRRSIYNLMSSLSSVVNESMASVFPKFIDRIMESVIFSEDMVPNVSDNADDDLALVDAPDIEIDLEHTDDEDDQDAYPVENDYIVEKEEAILSLKEFATHTGAAFAPYLQSAFENVYKMIDHPQGDVRMACIDSICSFITALHKLDDAAGLKRACEIAIPKFAHIMRTDDQVAVVLRMLDVLYDVFKYVPAINSQEHAELIFGCIRDIFTNKMACQFNEESGGGDDECSEESENDEMLFENAANLFPMFGLTLQPELFSLYFGRLYHFYIQRLAKVKERDLPEQRAYIYGALADCCKALKGCCATYFDALRPIFIAGSRDSDAKARQNSYFALGEIVFHSEEKSFESYPTILQALSEAIVRESVPAAMDNICGAVARLIVTNPDSVPLGQVLPVWLNHLPLKDDTVENDVIQKAFRVLYLKARPSIEAHLEQILAITIEASYKRQMPDVETTESAVALIKEIRANYPELFSKVSNMNPEVFNYVQAL

Target ORF information:

RefSeq Version	NM_001275027.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apollo, transcript variant B (Apl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275027.1

ORF Insert Sequence:

ATGGAGGCAG CTATTCTGGA TATAATCAAC GGGATCCTGA CCATAGACAC GGAACGAATC 
CGTGAGTCCA CAGCCAAGAT GCTAAAGGCC TACGAGAATC CCGATTCTCT GCTGGTTCTC 
ACCCAGATCG TGATGTCGGA CAGGCCGGTC CAAGAGCGGC AGGTCGCCGC CGTGCTCCTC 
AAGAGGCGGG TCAAAAAGTT ACGCCACTGG CAGTTGGTTC CCGCCGAGCA CCAGGCAGCA 
ATCAAATCGA ACATGCTACA GGTCCTCATC GCGGTGAAGG AAAAGACCGT GAAGGGCACG 
GTGGCCTTTA TAATTGGATC GCTGGTGCGC CACGAGGAGG GCGAGCAAAA CTCCTGGAGG 
GAGGAAATAC TGAAGTTTAT TTACGAACGT TGCAGCAGCC CCGATCCAAT AGAAAGCGAG 
CGGGGCAGCT CAATCTTTTC CTCGCTCATG GACGCTGCTC CCGATCAGTT TTCGGACCAC 
ACGGATACTA TGTTCCCATT GCTTGCCGGC ATTCTAGTTA CCGCCGAGGC GAATGGGAAC 
ATGGCCACAC CCACCGTGCA TAACATGCTG ACCGGTTCAT GCTTCTTGTT GCCTTTCGCC 
CTGGCCGCCT TTGTCGAAAA GGGGTACAGT ATTGAGTTCA TGGGTGCCTT TGATATCATC 
GACAGCATGG CCGAGCATGT GCCCCACTTG CTGACCGGTA ATGTGAAGCT GCTTCTGGAG 
TTTTGTCTGA TGATTGCGAG AAACAAGCAG TTCGACGCTT CGATTCGGGT TCAGGTGCTT 
ACCTTTGTAG GCAGCCTAGT TCGCCTCAAG AAGAAAATTA TTATGAAGCA GAAACTACTA 
CAGCCCACAC TATCTGTTCT TTTCGAAGTG ATTTGCCAGG ACGACCTCGA GGAAGACGAT 
GATTACTTTT CCTCGGAGAG CCTGAGTAGT CCATCCAATG CAGCTGCGCA GACACTGGAC 
CTGATGGCCC TTCACATGGT GCCGGACAAG TTTATTCCGC CACTACTGGA TTTGCTGGAG 
CCTGCGTTGC AGAGCCCGGA ACCTGTGCTT CGCCGTTCCT CTTTTATTTG CATGGGCGTT 
ATTGCCGAGG GCTGTTCCGA GGCCATTGGG AAAAAGTATC TCGAGGTCAT GCTAAATATC 
ATCAAAGCTG GAGTGTTGGA CTCTGTTATG TTCGTGCGGA CCGCCGCATT CTTCGCCCTC 
GGTCAGTTTT CCGAGTTTCT TCAGCCGACG ATCTGCAAGT TTGCTCCCCA AATTCTACCC 
GTGTTGTTCG ACTATCTAAA CCAGCTGGTA CTGGAACTCA AGGTTGGTGA GCCGGATTCG 
AAACATATGG ATCGTATGTT TTATGCCCTG GAGACCTTTT GCGAGAATCT GGATGAGGAT 
ATTGTTCCTT ATTTACCCAC ACTTATGGAT CGTCTGTTTG GGGTTATGGA ACCGCAGAAC 
TCAAATCAAA TGCGTGAAAT GGCTTTGTCG GCCATAGCAG CGGTGTCAGC AGCAGCTAAG 
GAAAACCTGA TGCCATATTT CCCCAGGATC ATGACCGTAC TACAGGGCTG TCTGGTGAAG 
GATTGCCCAA AAGAAATGTA CAGTCAGCGC ATCCAAGCTA TCGACACTCT GGCCGCGCTG 
TGTCGCGAAT TGGGCAAGGA TAATATTATT CCTCTTGCCG ACGATACAAT GAACTTCTGC 
CTGATGATGC TGGAAGACGG TCCGGATGAT CCCGAGTACC GCAGGAGTAT ATACAACCTG 
ATGTCCTCCC TGTCATCAGT TGTCAACGAG AGCATGGCCA GTGTGTTCCC CAAGTTCATT 
GATCGCATAA TGGAGTCTGT GATTTTTTCG GAGGACATGG TGCCAAATGT GTCGGACAAT 
GCGGACGATG ATTTGGCCCT AGTAGATGCC CCCGACATAG AGATTGATTT AGAGCACACA 
GACGACGAAG ATGACCAAGA CGCTTACCCG GTGGAGAATG ATTATATCGT CGAAAAGGAG 
GAGGCCATTC TGTCACTCAA GGAGTTTGCT ACCCATACCG GCGCCGCCTT CGCGCCCTAT 
TTGCAATCTG CATTCGAGAA CGTCTACAAG ATGATTGATC ATCCGCAAGG CGACGTTCGT 
ATGGCATGTA TTGACTCCAT TTGCTCGTTC ATTACGGCTC TCCACAAGTT GGATGATGCC 
GCCGGCCTGA AGCGCGCCTG CGAGATTGCC ATTCCAAAGT TTGCACATAT AATGCGCACC 
GACGACCAGG TTGCGGTCGT ACTCCGCATG CTTGACGTCC TCTACGATGT CTTCAAGTAC 
GTGCCGGCGA TAAACAGCCA GGAGCACGCC GAACTTATAT TCGGTTGCAT CAGGGACATC 
TTCACAAATA AAATGGCCTG TCAGTTTAAC GAGGAGAGCG GCGGCGGAGA TGATGAGTGT 
TCGGAGGAAA GCGAGAACGA CGAGATGCTG TTCGAGAACG CTGCCAATCT GTTCCCCATG 
TTTGGCTTAA CCCTTCAGCC GGAGCTGTTC TCGCTTTATT TTGGACGCCT TTACCATTTC 
TATATTCAAA GGCTGGCGAA GGTAAAGGAG CGCGATTTAC CAGAACAGCG GGCATACATC 
TATGGCGCAC TTGCTGACTG CTGCAAGGCG TTAAAAGGTT GCTGTGCTAC TTATTTCGAT 
GCCCTACGTC CCATTTTTAT CGCCGGTTCC AGGGATTCTG ATGCCAAAGC GCGACAGAAC 
TCCTATTTTG CACTAGGCGA AATTGTCTTC CATTCCGAGG AAAAATCATT CGAGTCTTAT 
CCGACAATTT TGCAAGCCCT TTCCGAAGCA ATTGTCAGGG AATCCGTTCC TGCCGCCATG 
GACAACATTT GCGGAGCAGT TGCTCGTCTC ATAGTTACCA ACCCAGATTC AGTGCCGCTT 
GGCCAGGTGC TGCCCGTGTG GCTCAACCAT TTGCCACTGA AGGATGACAC CGTGGAGAAC 
GATGTGATTC AGAAGGCGTT CCGTGTGCTA TACTTGAAGG CCCGCCCCAG CATTGAGGCA 
CATCTTGAGC AAATCCTGGC CATCACCATA GAGGCGAGTT ACAAGAGACA GATGCCTGAC 
GTCGAGACAA CCGAGAGCGC GGTGGCCCTC ATCAAAGAGA TTCGCGCCAA CTACCCGGAA 
CTGTTCAGCA AAGTATCGAA CATGAATCCA GAAGTGTTCA ATTATGTGCA AGCCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apl ( NM_001300155.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287084.1 Drosophila melanogaster apollo, transcript variant C (Apl), mRNA.

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CloneID	OFa20413
Clone ID Related Accession (Same CDS sequence)	NM_001275027.1 , NM_001300155.1
Accession Version	NM_001300155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	apollo, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCAG CTATTCTGGA TATAATCAAC GGGATCCTGA CCATAGACAC GGAACGAATC 
CGTGAGTCCA CAGCCAAGAT GCTAAAGGCC TACGAGAATC CCGATTCTCT GCTGGTTCTC 
ACCCAGATCG TGATGTCGGA CAGGCCGGTC CAAGAGCGGC AGGTCGCCGC CGTGCTCCTC 
AAGAGGCGGG TCAAAAAGTT ACGCCACTGG CAGTTGGTTC CCGCCGAGCA CCAGGCAGCA 
ATCAAATCGA ACATGCTACA GGTCCTCATC GCGGTGAAGG AAAAGACCGT GAAGGGCACG 
GTGGCCTTTA TAATTGGATC GCTGGTGCGC CACGAGGAGG GCGAGCAAAA CTCCTGGAGG 
GAGGAAATAC TGAAGTTTAT TTACGAACGT TGCAGCAGCC CCGATCCAAT AGAAAGCGAG 
CGGGGCAGCT CAATCTTTTC CTCGCTCATG GACGCTGCTC CCGATCAGTT TTCGGACCAC 
ACGGATACTA TGTTCCCATT GCTTGCCGGC ATTCTAGTTA CCGCCGAGGC GAATGGGAAC 
ATGGCCACAC CCACCGTGCA TAACATGCTG ACCGGTTCAT GCTTCTTGTT GCCTTTCGCC 
CTGGCCGCCT TTGTCGAAAA GGGGTACAGT ATTGAGTTCA TGGGTGCCTT TGATATCATC 
GACAGCATGG CCGAGCATGT GCCCCACTTG CTGACCGGTA ATGTGAAGCT GCTTCTGGAG 
TTTTGTCTGA TGATTGCGAG AAACAAGCAG TTCGACGCTT CGATTCGGGT TCAGGTGCTT 
ACCTTTGTAG GCAGCCTAGT TCGCCTCAAG AAGAAAATTA TTATGAAGCA GAAACTACTA 
CAGCCCACAC TATCTGTTCT TTTCGAAGTG ATTTGCCAGG ACGACCTCGA GGAAGACGAT 
GATTACTTTT CCTCGGAGAG CCTGAGTAGT CCATCCAATG CAGCTGCGCA GACACTGGAC 
CTGATGGCCC TTCACATGGT GCCGGACAAG TTTATTCCGC CACTACTGGA TTTGCTGGAG 
CCTGCGTTGC AGAGCCCGGA ACCTGTGCTT CGCCGTTCCT CTTTTATTTG CATGGGCGTT 
ATTGCCGAGG GCTGTTCCGA GGCCATTGGG AAAAAGTATC TCGAGGTCAT GCTAAATATC 
ATCAAAGCTG GAGTGTTGGA CTCTGTTATG TTCGTGCGGA CCGCCGCATT CTTCGCCCTC 
GGTCAGTTTT CCGAGTTTCT TCAGCCGACG ATCTGCAAGT TTGCTCCCCA AATTCTACCC 
GTGTTGTTCG ACTATCTAAA CCAGCTGGTA CTGGAACTCA AGGTTGGTGA GCCGGATTCG 
AAACATATGG ATCGTATGTT TTATGCCCTG GAGACCTTTT GCGAGAATCT GGATGAGGAT 
ATTGTTCCTT ATTTACCCAC ACTTATGGAT CGTCTGTTTG GGGTTATGGA ACCGCAGAAC 
TCAAATCAAA TGCGTGAAAT GGCTTTGTCG GCCATAGCAG CGGTGTCAGC AGCAGCTAAG 
GAAAACCTGA TGCCATATTT CCCCAGGATC ATGACCGTAC TACAGGGCTG TCTGGTGAAG 
GATTGCCCAA AAGAAATGTA CAGTCAGCGC ATCCAAGCTA TCGACACTCT GGCCGCGCTG 
TGTCGCGAAT TGGGCAAGGA TAATATTATT CCTCTTGCCG ACGATACAAT GAACTTCTGC 
CTGATGATGC TGGAAGACGG TCCGGATGAT CCCGAGTACC GCAGGAGTAT ATACAACCTG 
ATGTCCTCCC TGTCATCAGT TGTCAACGAG AGCATGGCCA GTGTGTTCCC CAAGTTCATT 
GATCGCATAA TGGAGTCTGT GATTTTTTCG GAGGACATGG TGCCAAATGT GTCGGACAAT 
GCGGACGATG ATTTGGCCCT AGTAGATGCC CCCGACATAG AGATTGATTT AGAGCACACA 
GACGACGAAG ATGACCAAGA CGCTTACCCG GTGGAGAATG ATTATATCGT CGAAAAGGAG 
GAGGCCATTC TGTCACTCAA GGAGTTTGCT ACCCATACCG GCGCCGCCTT CGCGCCCTAT 
TTGCAATCTG CATTCGAGAA CGTCTACAAG ATGATTGATC ATCCGCAAGG CGACGTTCGT 
ATGGCATGTA TTGACTCCAT TTGCTCGTTC ATTACGGCTC TCCACAAGTT GGATGATGCC 
GCCGGCCTGA AGCGCGCCTG CGAGATTGCC ATTCCAAAGT TTGCACATAT AATGCGCACC 
GACGACCAGG TTGCGGTCGT ACTCCGCATG CTTGACGTCC TCTACGATGT CTTCAAGTAC 
GTGCCGGCGA TAAACAGCCA GGAGCACGCC GAACTTATAT TCGGTTGCAT CAGGGACATC 
TTCACAAATA AAATGGCCTG TCAGTTTAAC GAGGAGAGCG GCGGCGGAGA TGATGAGTGT 
TCGGAGGAAA GCGAGAACGA CGAGATGCTG TTCGAGAACG CTGCCAATCT GTTCCCCATG 
TTTGGCTTAA CCCTTCAGCC GGAGCTGTTC TCGCTTTATT TTGGACGCCT TTACCATTTC 
TATATTCAAA GGCTGGCGAA GGTAAAGGAG CGCGATTTAC CAGAACAGCG GGCATACATC 
TATGGCGCAC TTGCTGACTG CTGCAAGGCG TTAAAAGGTT GCTGTGCTAC TTATTTCGAT 
GCCCTACGTC CCATTTTTAT CGCCGGTTCC AGGGATTCTG ATGCCAAAGC GCGACAGAAC 
TCCTATTTTG CACTAGGCGA AATTGTCTTC CATTCCGAGG AAAAATCATT CGAGTCTTAT 
CCGACAATTT TGCAAGCCCT TTCCGAAGCA ATTGTCAGGG AATCCGTTCC TGCCGCCATG 
GACAACATTT GCGGAGCAGT TGCTCGTCTC ATAGTTACCA ACCCAGATTC AGTGCCGCTT 
GGCCAGGTGC TGCCCGTGTG GCTCAACCAT TTGCCACTGA AGGATGACAC CGTGGAGAAC 
GATGTGATTC AGAAGGCGTT CCGTGTGCTA TACTTGAAGG CCCGCCCCAG CATTGAGGCA 
CATCTTGAGC AAATCCTGGC CATCACCATA GAGGCGAGTT ACAAGAGACA GATGCCTGAC 
GTCGAGACAA CCGAGAGCGC GGTGGCCCTC ATCAAAGAGA TTCGCGCCAA CTACCCGGAA 
CTGTTCAGCA AAGTATCGAA CATGAATCCA GAAGTGTTCA ATTATGTGCA AGCCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287084.1
CDS	354..3533
Translation	MEAAILDIINGILTIDTERIRESTAKMLKAYENPDSLLVLTQIVMSDRPVQERQVAAVLLKRRVKKLRHWQLVPAEHQAAIKSNMLQVLIAVKEKTVKGTVAFIIGSLVRHEEGEQNSWREEILKFIYERCSSPDPIESERGSSIFSSLMDAAPDQFSDHTDTMFPLLAGILVTAEANGNMATPTVHNMLTGSCFLLPFALAAFVEKGYSIEFMGAFDIIDSMAEHVPHLLTGNVKLLLEFCLMIARNKQFDASIRVQVLTFVGSLVRLKKKIIMKQKLLQPTLSVLFEVICQDDLEEDDDYFSSESLSSPSNAAAQTLDLMALHMVPDKFIPPLLDLLEPALQSPEPVLRRSSFICMGVIAEGCSEAIGKKYLEVMLNIIKAGVLDSVMFVRTAAFFALGQFSEFLQPTICKFAPQILPVLFDYLNQLVLELKVGEPDSKHMDRMFYALETFCENLDEDIVPYLPTLMDRLFGVMEPQNSNQMREMALSAIAAVSAAAKENLMPYFPRIMTVLQGCLVKDCPKEMYSQRIQAIDTLAALCRELGKDNIIPLADDTMNFCLMMLEDGPDDPEYRRSIYNLMSSLSSVVNESMASVFPKFIDRIMESVIFSEDMVPNVSDNADDDLALVDAPDIEIDLEHTDDEDDQDAYPVENDYIVEKEEAILSLKEFATHTGAAFAPYLQSAFENVYKMIDHPQGDVRMACIDSICSFITALHKLDDAAGLKRACEIAIPKFAHIMRTDDQVAVVLRMLDVLYDVFKYVPAINSQEHAELIFGCIRDIFTNKMACQFNEESGGGDDECSEESENDEMLFENAANLFPMFGLTLQPELFSLYFGRLYHFYIQRLAKVKERDLPEQRAYIYGALADCCKALKGCCATYFDALRPIFIAGSRDSDAKARQNSYFALGEIVFHSEEKSFESYPTILQALSEAIVRESVPAAMDNICGAVARLIVTNPDSVPLGQVLPVWLNHLPLKDDTVENDVIQKAFRVLYLKARPSIEAHLEQILAITIEASYKRQMPDVETTESAVALIKEIRANYPELFSKVSNMNPEVFNYVQAL

Target ORF information:

RefSeq Version	NM_001300155.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster apollo, transcript variant C (Apl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300155.1

ORF Insert Sequence:

ATGGAGGCAG CTATTCTGGA TATAATCAAC GGGATCCTGA CCATAGACAC GGAACGAATC 
CGTGAGTCCA CAGCCAAGAT GCTAAAGGCC TACGAGAATC CCGATTCTCT GCTGGTTCTC 
ACCCAGATCG TGATGTCGGA CAGGCCGGTC CAAGAGCGGC AGGTCGCCGC CGTGCTCCTC 
AAGAGGCGGG TCAAAAAGTT ACGCCACTGG CAGTTGGTTC CCGCCGAGCA CCAGGCAGCA 
ATCAAATCGA ACATGCTACA GGTCCTCATC GCGGTGAAGG AAAAGACCGT GAAGGGCACG 
GTGGCCTTTA TAATTGGATC GCTGGTGCGC CACGAGGAGG GCGAGCAAAA CTCCTGGAGG 
GAGGAAATAC TGAAGTTTAT TTACGAACGT TGCAGCAGCC CCGATCCAAT AGAAAGCGAG 
CGGGGCAGCT CAATCTTTTC CTCGCTCATG GACGCTGCTC CCGATCAGTT TTCGGACCAC 
ACGGATACTA TGTTCCCATT GCTTGCCGGC ATTCTAGTTA CCGCCGAGGC GAATGGGAAC 
ATGGCCACAC CCACCGTGCA TAACATGCTG ACCGGTTCAT GCTTCTTGTT GCCTTTCGCC 
CTGGCCGCCT TTGTCGAAAA GGGGTACAGT ATTGAGTTCA TGGGTGCCTT TGATATCATC 
GACAGCATGG CCGAGCATGT GCCCCACTTG CTGACCGGTA ATGTGAAGCT GCTTCTGGAG 
TTTTGTCTGA TGATTGCGAG AAACAAGCAG TTCGACGCTT CGATTCGGGT TCAGGTGCTT 
ACCTTTGTAG GCAGCCTAGT TCGCCTCAAG AAGAAAATTA TTATGAAGCA GAAACTACTA 
CAGCCCACAC TATCTGTTCT TTTCGAAGTG ATTTGCCAGG ACGACCTCGA GGAAGACGAT 
GATTACTTTT CCTCGGAGAG CCTGAGTAGT CCATCCAATG CAGCTGCGCA GACACTGGAC 
CTGATGGCCC TTCACATGGT GCCGGACAAG TTTATTCCGC CACTACTGGA TTTGCTGGAG 
CCTGCGTTGC AGAGCCCGGA ACCTGTGCTT CGCCGTTCCT CTTTTATTTG CATGGGCGTT 
ATTGCCGAGG GCTGTTCCGA GGCCATTGGG AAAAAGTATC TCGAGGTCAT GCTAAATATC 
ATCAAAGCTG GAGTGTTGGA CTCTGTTATG TTCGTGCGGA CCGCCGCATT CTTCGCCCTC 
GGTCAGTTTT CCGAGTTTCT TCAGCCGACG ATCTGCAAGT TTGCTCCCCA AATTCTACCC 
GTGTTGTTCG ACTATCTAAA CCAGCTGGTA CTGGAACTCA AGGTTGGTGA GCCGGATTCG 
AAACATATGG ATCGTATGTT TTATGCCCTG GAGACCTTTT GCGAGAATCT GGATGAGGAT 
ATTGTTCCTT ATTTACCCAC ACTTATGGAT CGTCTGTTTG GGGTTATGGA ACCGCAGAAC 
TCAAATCAAA TGCGTGAAAT GGCTTTGTCG GCCATAGCAG CGGTGTCAGC AGCAGCTAAG 
GAAAACCTGA TGCCATATTT CCCCAGGATC ATGACCGTAC TACAGGGCTG TCTGGTGAAG 
GATTGCCCAA AAGAAATGTA CAGTCAGCGC ATCCAAGCTA TCGACACTCT GGCCGCGCTG 
TGTCGCGAAT TGGGCAAGGA TAATATTATT CCTCTTGCCG ACGATACAAT GAACTTCTGC 
CTGATGATGC TGGAAGACGG TCCGGATGAT CCCGAGTACC GCAGGAGTAT ATACAACCTG 
ATGTCCTCCC TGTCATCAGT TGTCAACGAG AGCATGGCCA GTGTGTTCCC CAAGTTCATT 
GATCGCATAA TGGAGTCTGT GATTTTTTCG GAGGACATGG TGCCAAATGT GTCGGACAAT 
GCGGACGATG ATTTGGCCCT AGTAGATGCC CCCGACATAG AGATTGATTT AGAGCACACA 
GACGACGAAG ATGACCAAGA CGCTTACCCG GTGGAGAATG ATTATATCGT CGAAAAGGAG 
GAGGCCATTC TGTCACTCAA GGAGTTTGCT ACCCATACCG GCGCCGCCTT CGCGCCCTAT 
TTGCAATCTG CATTCGAGAA CGTCTACAAG ATGATTGATC ATCCGCAAGG CGACGTTCGT 
ATGGCATGTA TTGACTCCAT TTGCTCGTTC ATTACGGCTC TCCACAAGTT GGATGATGCC 
GCCGGCCTGA AGCGCGCCTG CGAGATTGCC ATTCCAAAGT TTGCACATAT AATGCGCACC 
GACGACCAGG TTGCGGTCGT ACTCCGCATG CTTGACGTCC TCTACGATGT CTTCAAGTAC 
GTGCCGGCGA TAAACAGCCA GGAGCACGCC GAACTTATAT TCGGTTGCAT CAGGGACATC 
TTCACAAATA AAATGGCCTG TCAGTTTAAC GAGGAGAGCG GCGGCGGAGA TGATGAGTGT 
TCGGAGGAAA GCGAGAACGA CGAGATGCTG TTCGAGAACG CTGCCAATCT GTTCCCCATG 
TTTGGCTTAA CCCTTCAGCC GGAGCTGTTC TCGCTTTATT TTGGACGCCT TTACCATTTC 
TATATTCAAA GGCTGGCGAA GGTAAAGGAG CGCGATTTAC CAGAACAGCG GGCATACATC 
TATGGCGCAC TTGCTGACTG CTGCAAGGCG TTAAAAGGTT GCTGTGCTAC TTATTTCGAT 
GCCCTACGTC CCATTTTTAT CGCCGGTTCC AGGGATTCTG ATGCCAAAGC GCGACAGAAC 
TCCTATTTTG CACTAGGCGA AATTGTCTTC CATTCCGAGG AAAAATCATT CGAGTCTTAT 
CCGACAATTT TGCAAGCCCT TTCCGAAGCA ATTGTCAGGG AATCCGTTCC TGCCGCCATG 
GACAACATTT GCGGAGCAGT TGCTCGTCTC ATAGTTACCA ACCCAGATTC AGTGCCGCTT 
GGCCAGGTGC TGCCCGTGTG GCTCAACCAT TTGCCACTGA AGGATGACAC CGTGGAGAAC 
GATGTGATTC AGAAGGCGTT CCGTGTGCTA TACTTGAAGG CCCGCCCCAG CATTGAGGCA 
CATCTTGAGC AAATCCTGGC CATCACCATA GAGGCGAGTT ACAAGAGACA GATGCCTGAC 
GTCGAGACAA CCGAGAGCGC GGTGGCCCTC ATCAAAGAGA TTCGCGCCAA CTACCCGGAA 
CTGTTCAGCA AAGTATCGAA CATGAATCCA GAAGTGTTCA ATTATGTGCA AGCCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Apl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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