ND-MWFE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ND-MWFE
Entrez Gene ID	5740593
Full Name	NADH dehydrogenase (ubiquinone) MWFE subunit
Synonyms	BcDNA:RH49308,CG13328,CG17054,CG30057,CG34439,Dmel\CG34439,l(2)c00093
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) MWFE subunit Names CG34439 gene product from transcript CG34439-RB CG34439-PA CG34439-PB NADH ubiquinone oxidoreductase MWFE ND-MWFE-PA ND-MWFE-PB lethal (2) c00093
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F4-49F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103827.2	NP_001097297.1	NADH dehydrogenase (ubiquinone) MWFE subunit, isoform B
NM_001103826.2	NP_001097296.1	NADH dehydrogenase (ubiquinone) MWFE subunit, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ND-MWFE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ND-MWFE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11919	NM_001103826.2 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) MWFE subunit (ND-MWFE), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11918	NM_001103827.2 Latest version!	Drosophila melanogaster NADH dehydrogenase (ubiquinone) MWFE subunit (ND-MWFE), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ND-MWFE ( NM_001103826.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097296.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) MWFE subunit (ND-MWFE), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11919
Clone ID Related Accession (Same CDS sequence)	NM_001103826.2
Accession Version	NM_001103826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) MWFE subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103826.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGTTCG AAATCCTACC TGGTGCGGTG ATCATCACCA CGCTCCTCTC GGTGCCCATA 
TACGCCATGT ACGGCCTGGA CAAGCTGATG ATCGGCAATG CTTTCCGGCG CAACATGGAC 
GAGCGTTTCA GCCGAGTTAT GTACCAGCGC GATTTCCGAC TGACCGACAA TCCCTACAAG 
ATGAACGGTC TGGATGCCAT ACCGGATGAG AAAACGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097296.1
CDS	83..304
Translation	MWFEILPGAVIITTLLSVPIYAMYGLDKLMIGNAFRRNMDERFSRVMYQRDFRLTDNPYKMNGLDAIPDEKTN

Target ORF information:

RefSeq Version	NM_001103826.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) MWFE subunit (ND-MWFE), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103826.2

ORF Insert Sequence:

ATGTGGTTCG AAATCCTACC TGGTGCGGTG ATCATCACCA CGCTCCTCTC GGTGCCCATA 
TACGCCATGT ACGGCCTGGA CAAGCTGATG ATCGGCAATG CTTTCCGGCG CAACATGGAC 
GAGCGTTTCA GCCGAGTTAT GTACCAGCGC GATTTCCGAC TGACCGACAA TCCCTACAAG 
ATGAACGGTC TGGATGCCAT ACCGGATGAG AAAACGAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ND-MWFE ( NM_001103827.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097297.1 Drosophila melanogaster NADH dehydrogenase (ubiquinone) MWFE subunit (ND-MWFE), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11918
Clone ID Related Accession (Same CDS sequence)	NM_001103827.2
Accession Version	NM_001103827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NADH dehydrogenase (ubiquinone) MWFE subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103827.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGTTCG AAATCCTACC TGGTGCGGTG ATCATCACCA CGCTCCTCTC GGTGCCCATA 
TACGCCATGT ACGGCCTGGA CAAGCTGATG ATCGGCAATG CTTTCCGGCG CAACATGGAC 
GAGCGTTTCA GCCGAGTTAT GTACCAGCGC GATTTCCGAC TGACCGACAA TCCCTACAAG 
ATGAACGGTC TTGAACAAAT TCCCGATGAA GAGGTGAAGA AGGAGGAGAA GGACCCCAAT 
GAGGACAGCG ATGATCCCGC AATCGTGAAG AAGCGGGAGA AAGAGCGGAA GCTAAGGGAG 
AAACAGCTGA AGAAGGAGGA AAAGCTCAGG GAGAAACAGC TGAAGGAGGA GGAGAAGCAG 
AAGAAAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097297.1
CDS	83..454
Translation	MWFEILPGAVIITTLLSVPIYAMYGLDKLMIGNAFRRNMDERFSRVMYQRDFRLTDNPYKMNGLEQIPDEEVKKEEKDPNEDSDDPAIVKKREKERKLREKQLKKEEKLREKQLKEEEKQKKN

Target ORF information:

RefSeq Version	NM_001103827.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NADH dehydrogenase (ubiquinone) MWFE subunit (ND-MWFE), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103827.2

ORF Insert Sequence:

ATGTGGTTCG AAATCCTACC TGGTGCGGTG ATCATCACCA CGCTCCTCTC GGTGCCCATA 
TACGCCATGT ACGGCCTGGA CAAGCTGATG ATCGGCAATG CTTTCCGGCG CAACATGGAC 
GAGCGTTTCA GCCGAGTTAT GTACCAGCGC GATTTCCGAC TGACCGACAA TCCCTACAAG 
ATGAACGGTC TTGAACAAAT TCCCGATGAA GAGGTGAAGA AGGAGGAGAA GGACCCCAAT 
GAGGACAGCG ATGATCCCGC AATCGTGAAG AAGCGGGAGA AAGAGCGGAA GCTAAGGGAG 
AAACAGCTGA AGAAGGAGGA AAAGCTCAGG GAGAAACAGC TGAAGGAGGA GGAGAAGCAG 
AAGAAAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ND-MWFE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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