Not1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Not1
Entrez Gene ID	5740252
Full Name	CG34407 gene product from transcript CG34407-RG
Synonyms	CG1874,CG1884,CG34407,Dmel\CG34407,Dmel_CG1874,Dmel_CG1884,NOT,NOT1,anon-EST:Liang-1.29,anon-WO0118547.221,clone 1.29,not1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45F4-45F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103772.3	NP_001097242.1	Not1, isoform C
NM_001103773.3	NP_001097243.1	Not1, isoform D
NM_001103774.3	NP_001097244.1	Not1, isoform E
NM_001299323.1	NP_001286252.1	Not1, isoform H
NM_001169628.2	NP_001163099.1	Not1, isoform F
NM_165678.4	NP_724798.3	Not1, isoform G

Pathways from BioSystems

Homologies

Homo sapiens (human)	CNOT1	NP_057368.3
Mus musculus (house mouse)	Cnot1	NP_835179.1
Danio rerio (zebrafish)	cnot1	NP_001073420.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009057	XP_319808.4
Drosophila melanogaster (fruit fly)	Not1	NP_724798.3
Caenorhabditis elegans (roundworm)	let-711	NP_498516.2
Oryza sativa (rice)	Os10g0556801	NP_001176268.1
Pan troglodytes (chimpanzee)	CNOT1	XP_511007.2
Bos taurus (cattle)	CNOT1	NP_001192968.1
Gallus gallus (chicken)	CNOT1	NP_001264062.1
Rattus norvegicus (Norway rat)	Cnot1	NP_001128312.1
	CNOT1	XP_001102008.2
Canis lupus familiaris (dog)	CNOT1	XP_535279.4
Arabidopsis thaliana (thale cress)	AT1G02080	NP_171710.4
Xenopus tropicalis (tropical clawed frog)	cnot1	NP_001090658.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of the NED central region of Nanos bound to the NOT module reveals an unanticipated bipartite binding interface that contacts NOT1 and NOT3 and is distinct from the NOT1 interacting motif of vertebrate Nanos.
Title: Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos.

These results indicate that the Ccr4-Not complex controls expression of 4E-BP at multiple levels and adjusts the magnitude of the total effect.
Title: Multi-functional regulation of 4E-BP gene expression by the Ccr4-Not complex.

The following Not1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Not1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11876	NM_001103772.3 Latest version!	Drosophila melanogaster Not1, transcript variant C (Not1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa11876	NM_165678.4 Latest version!	Drosophila melanogaster Not1, transcript variant G (Not1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa11877	NM_001103773.3 Latest version!	Drosophila melanogaster Not1, transcript variant D (Not1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa11878	NM_001103774.3 Latest version!	Drosophila melanogaster Not1, transcript variant E (Not1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa12480	NM_001169628.2 Latest version!	Drosophila melanogaster Not1, transcript variant F (Not1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa22408	NM_001299323.1 Latest version!	Drosophila melanogaster Not1, transcript variant H (Not1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Not1 ( NM_001103772.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097242.1 Drosophila melanogaster Not1, transcript variant C (Not1), mRNA.

Price & Availability↑

CloneID	OFa11876
Clone ID Related Accession (Same CDS sequence)	NM_001103772.3 , NM_165678.4
Accession Version	NM_001103772.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Not1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103772.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CGTAAGGTCA 
TGCGATACAG ATGATCCGCC GGGCTTACAA GAGAAGACGG AGTTTCTGCT TAAGGATTGG 
GTTGCACTGT ACACGCAACA GAACCAGCAG TCAACGCGCG ATGCACGCAA CTTCGGTGCA 
TTTGTTCAGA AGATGAACAC ATATGGTATC CTGAAAACGG ACGACTTGAT CACCCGTTTT 
TTCCGCCAGG CCACACACAT TTGCACGGAT GTGGTGTACA GAATGTTCGC CGAGCCGAGT 
TTACCAATCA ACCAGGCCAA AAACAAGATA TTCCAGTGGA TCGATGCGTT CGTCCATCTG 
ATTGCAATGC TCGTGCGACA CTCAGGCGAA GCAGGTAATC CAACCACCAA GATCAACCTG 
TTAAACAAGG TGCTGGGCAT TGTGCTGGGA ACGCTGATTA AGGACCATGA GATGCGTGGC 
GTTAGCTTCC AGCAGGTTGG CTACCATCGC TTCTTCATGA TGCTGTTCAT GGAGCTGTGC 
ACGGCCGATG TGATTCTCGA GTCCCTGATG CACAGTATTG TGTCGGCCTT CGCCTACACA 
TATCACTTGT TGAATCCCAG CGTGGCTCCG GGATTCTGCT TTGCTTGGTT GGAGCTCATT 
TCGCATCGCG TCTTCCTGGG CCGCATCCTG GTCCAAATTC CCGGGCAAAA GGGCTGGCCA 
CTTTACGCTC AGTTGCTGCA GGACCTTTTT AAATATCTTG CGCCCTTCTT ACGCAACACT 
GAGCTCGGCA AACCAGTCCA GCTTTTGTAC AAGGGGACGC TACGTGTTCT GCTCGTTCTG 
CTTCACGACT TCCCGGAGTT CTTGTGCGAC TATCATTTTG GGTTCTGTGA CACCATCCCG 
CCCAATTGCG TCCAGATGCG TAACATCATC CTGTCGGCGT TTCCGCGCAA CATGCGCTTG 
CCCGATCCCT TCACGCCCAA CCTAAAAGTG GACATGCTGT CGGACAGTAG TAATGCGCCT 
AAGGTGCTCA GTAGCTACAT TATGAACATC CAGCCCGCGA ACTTTAAGAA GGATTTGGAC 
TCGTACCTCA AGGCCCGGGC GCCAGTTACT TTTCTATCGG AATTACGTGG TCACTTGCAG 
GTGACAAGCG AGCCAGGAAC TCGTTACAAC ATGGCACTGA TGAATGCTCT GGTCATGTAT 
GTGGGAACTC AGGCGATTGC TTTGATCAGA AACAAAAACT TTGTGCCCAA CACCTCGAAC 
ATAGCACACA GCGCCCACAT GGATATCTTC CAGAATCTTG CTGTTGACTT GGATACGGAG 
GGTCGCTATC TGTTCTTGAA TGCGATTGCG AACCAGTTGC GCTATCCCAA CAGCCACACG 
CACTACTTCA GCTGTGCGGT GCTGCATCTG TTTGCCGAGG CCAACTCGGA GGCAATTCAG 
GAGCAGATCA CTCGCGTACT TCTGGAACGA TTGATCGTGA ACCGCCCGCA TCCTTGGGGA 
CTGTTGATCA CTTTTATTGA GCTGATCAAA AATCCCATCT ACAAGTTCTG GGACCATGAC 
TTTGTGCACT GTGCGCCAGA GATCACCAAG CTCTTTGAAT CGGTGGCCCG TTCCTGTCTG 
GCCAAGTCGA ACGTCACCCA GCAGTTGAAC ATGCCCGTCG TCGATGGCGA GGGCCAGGAG 
GTAGCCACCA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097242.1
CDS	719..8236
Translation	MNVESQLKTPPTHIRNLVKHVNKRNFNESSEQINQFVKEHGLEADRSSLRHLFSVINFSDLVPSVTVQLQAKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKSLKPVAVADLVGQVARLTGINKVCECVFALALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCLSEYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEADALNEVGIEDIYDHLSEIIFTNQGKNNIMDTSWINLILEIGYEFTSSVEECKNHLCSRERERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFNQLIPTFLGNHPNSNVILASAWSSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPEDQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVPPPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGPGMDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAAAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKALLSGLNGMPSMAEIQAAAMQLASENVELVCAFIQKTSAEKAAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSIPGFQQMSDRDIALFVPKPTDLSQPNVFANDDSMVYGELASKMEAFMNTAIGVPTLQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNNSFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSSSDYSPFNGNDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQAKNKIFQWIDAFVHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEGQEVATIN

Target ORF information:

RefSeq Version	NM_001103772.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Not1, transcript variant C (Not1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103772.3

ORF Insert Sequence:

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CGTAAGGTCA 
TGCGATACAG ATGATCCGCC GGGCTTACAA GAGAAGACGG AGTTTCTGCT TAAGGATTGG 
GTTGCACTGT ACACGCAACA GAACCAGCAG TCAACGCGCG ATGCACGCAA CTTCGGTGCA 
TTTGTTCAGA AGATGAACAC ATATGGTATC CTGAAAACGG ACGACTTGAT CACCCGTTTT 
TTCCGCCAGG CCACACACAT TTGCACGGAT GTGGTGTACA GAATGTTCGC CGAGCCGAGT 
TTACCAATCA ACCAGGCCAA AAACAAGATA TTCCAGTGGA TCGATGCGTT CGTCCATCTG 
ATTGCAATGC TCGTGCGACA CTCAGGCGAA GCAGGTAATC CAACCACCAA GATCAACCTG 
TTAAACAAGG TGCTGGGCAT TGTGCTGGGA ACGCTGATTA AGGACCATGA GATGCGTGGC 
GTTAGCTTCC AGCAGGTTGG CTACCATCGC TTCTTCATGA TGCTGTTCAT GGAGCTGTGC 
ACGGCCGATG TGATTCTCGA GTCCCTGATG CACAGTATTG TGTCGGCCTT CGCCTACACA 
TATCACTTGT TGAATCCCAG CGTGGCTCCG GGATTCTGCT TTGCTTGGTT GGAGCTCATT 
TCGCATCGCG TCTTCCTGGG CCGCATCCTG GTCCAAATTC CCGGGCAAAA GGGCTGGCCA 
CTTTACGCTC AGTTGCTGCA GGACCTTTTT AAATATCTTG CGCCCTTCTT ACGCAACACT 
GAGCTCGGCA AACCAGTCCA GCTTTTGTAC AAGGGGACGC TACGTGTTCT GCTCGTTCTG 
CTTCACGACT TCCCGGAGTT CTTGTGCGAC TATCATTTTG GGTTCTGTGA CACCATCCCG 
CCCAATTGCG TCCAGATGCG TAACATCATC CTGTCGGCGT TTCCGCGCAA CATGCGCTTG 
CCCGATCCCT TCACGCCCAA CCTAAAAGTG GACATGCTGT CGGACAGTAG TAATGCGCCT 
AAGGTGCTCA GTAGCTACAT TATGAACATC CAGCCCGCGA ACTTTAAGAA GGATTTGGAC 
TCGTACCTCA AGGCCCGGGC GCCAGTTACT TTTCTATCGG AATTACGTGG TCACTTGCAG 
GTGACAAGCG AGCCAGGAAC TCGTTACAAC ATGGCACTGA TGAATGCTCT GGTCATGTAT 
GTGGGAACTC AGGCGATTGC TTTGATCAGA AACAAAAACT TTGTGCCCAA CACCTCGAAC 
ATAGCACACA GCGCCCACAT GGATATCTTC CAGAATCTTG CTGTTGACTT GGATACGGAG 
GGTCGCTATC TGTTCTTGAA TGCGATTGCG AACCAGTTGC GCTATCCCAA CAGCCACACG 
CACTACTTCA GCTGTGCGGT GCTGCATCTG TTTGCCGAGG CCAACTCGGA GGCAATTCAG 
GAGCAGATCA CTCGCGTACT TCTGGAACGA TTGATCGTGA ACCGCCCGCA TCCTTGGGGA 
CTGTTGATCA CTTTTATTGA GCTGATCAAA AATCCCATCT ACAAGTTCTG GGACCATGAC 
TTTGTGCACT GTGCGCCAGA GATCACCAAG CTCTTTGAAT CGGTGGCCCG TTCCTGTCTG 
GCCAAGTCGA ACGTCACCCA GCAGTTGAAC ATGCCCGTCG TCGATGGCGA GGGCCAGGAG 
GTAGCCACCA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Not1 ( NM_165678.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724798.3 Drosophila melanogaster Not1, transcript variant G (Not1), mRNA.

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CloneID	OFa11876
Clone ID Related Accession (Same CDS sequence)	NM_001103772.3 , NM_165678.4
Accession Version	NM_165678.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Not1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165678.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CGTAAGGTCA 
TGCGATACAG ATGATCCGCC GGGCTTACAA GAGAAGACGG AGTTTCTGCT TAAGGATTGG 
GTTGCACTGT ACACGCAACA GAACCAGCAG TCAACGCGCG ATGCACGCAA CTTCGGTGCA 
TTTGTTCAGA AGATGAACAC ATATGGTATC CTGAAAACGG ACGACTTGAT CACCCGTTTT 
TTCCGCCAGG CCACACACAT TTGCACGGAT GTGGTGTACA GAATGTTCGC CGAGCCGAGT 
TTACCAATCA ACCAGGCCAA AAACAAGATA TTCCAGTGGA TCGATGCGTT CGTCCATCTG 
ATTGCAATGC TCGTGCGACA CTCAGGCGAA GCAGGTAATC CAACCACCAA GATCAACCTG 
TTAAACAAGG TGCTGGGCAT TGTGCTGGGA ACGCTGATTA AGGACCATGA GATGCGTGGC 
GTTAGCTTCC AGCAGGTTGG CTACCATCGC TTCTTCATGA TGCTGTTCAT GGAGCTGTGC 
ACGGCCGATG TGATTCTCGA GTCCCTGATG CACAGTATTG TGTCGGCCTT CGCCTACACA 
TATCACTTGT TGAATCCCAG CGTGGCTCCG GGATTCTGCT TTGCTTGGTT GGAGCTCATT 
TCGCATCGCG TCTTCCTGGG CCGCATCCTG GTCCAAATTC CCGGGCAAAA GGGCTGGCCA 
CTTTACGCTC AGTTGCTGCA GGACCTTTTT AAATATCTTG CGCCCTTCTT ACGCAACACT 
GAGCTCGGCA AACCAGTCCA GCTTTTGTAC AAGGGGACGC TACGTGTTCT GCTCGTTCTG 
CTTCACGACT TCCCGGAGTT CTTGTGCGAC TATCATTTTG GGTTCTGTGA CACCATCCCG 
CCCAATTGCG TCCAGATGCG TAACATCATC CTGTCGGCGT TTCCGCGCAA CATGCGCTTG 
CCCGATCCCT TCACGCCCAA CCTAAAAGTG GACATGCTGT CGGACAGTAG TAATGCGCCT 
AAGGTGCTCA GTAGCTACAT TATGAACATC CAGCCCGCGA ACTTTAAGAA GGATTTGGAC 
TCGTACCTCA AGGCCCGGGC GCCAGTTACT TTTCTATCGG AATTACGTGG TCACTTGCAG 
GTGACAAGCG AGCCAGGAAC TCGTTACAAC ATGGCACTGA TGAATGCTCT GGTCATGTAT 
GTGGGAACTC AGGCGATTGC TTTGATCAGA AACAAAAACT TTGTGCCCAA CACCTCGAAC 
ATAGCACACA GCGCCCACAT GGATATCTTC CAGAATCTTG CTGTTGACTT GGATACGGAG 
GGTCGCTATC TGTTCTTGAA TGCGATTGCG AACCAGTTGC GCTATCCCAA CAGCCACACG 
CACTACTTCA GCTGTGCGGT GCTGCATCTG TTTGCCGAGG CCAACTCGGA GGCAATTCAG 
GAGCAGATCA CTCGCGTACT TCTGGAACGA TTGATCGTGA ACCGCCCGCA TCCTTGGGGA 
CTGTTGATCA CTTTTATTGA GCTGATCAAA AATCCCATCT ACAAGTTCTG GGACCATGAC 
TTTGTGCACT GTGCGCCAGA GATCACCAAG CTCTTTGAAT CGGTGGCCCG TTCCTGTCTG 
GCCAAGTCGA ACGTCACCCA GCAGTTGAAC ATGCCCGTCG TCGATGGCGA GGGCCAGGAG 
GTAGCCACCA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724798.3
CDS	719..8236
Translation	MNVESQLKTPPTHIRNLVKHVNKRNFNESSEQINQFVKEHGLEADRSSLRHLFSVINFSDLVPSVTVQLQAKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKSLKPVAVADLVGQVARLTGINKVCECVFALALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCLSEYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEADALNEVGIEDIYDHLSEIIFTNQGKNNIMDTSWINLILEIGYEFTSSVEECKNHLCSRERERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFNQLIPTFLGNHPNSNVILASAWSSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPEDQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVPPPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGPGMDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAAAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKALLSGLNGMPSMAEIQAAAMQLASENVELVCAFIQKTSAEKAAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSIPGFQQMSDRDIALFVPKPTDLSQPNVFANDDSMVYGELASKMEAFMNTAIGVPTLQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNNSFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSSSDYSPFNGNDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQAKNKIFQWIDAFVHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEGQEVATIN

Target ORF information:

RefSeq Version	NM_165678.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Not1, transcript variant G (Not1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165678.4

ORF Insert Sequence:

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CGTAAGGTCA 
TGCGATACAG ATGATCCGCC GGGCTTACAA GAGAAGACGG AGTTTCTGCT TAAGGATTGG 
GTTGCACTGT ACACGCAACA GAACCAGCAG TCAACGCGCG ATGCACGCAA CTTCGGTGCA 
TTTGTTCAGA AGATGAACAC ATATGGTATC CTGAAAACGG ACGACTTGAT CACCCGTTTT 
TTCCGCCAGG CCACACACAT TTGCACGGAT GTGGTGTACA GAATGTTCGC CGAGCCGAGT 
TTACCAATCA ACCAGGCCAA AAACAAGATA TTCCAGTGGA TCGATGCGTT CGTCCATCTG 
ATTGCAATGC TCGTGCGACA CTCAGGCGAA GCAGGTAATC CAACCACCAA GATCAACCTG 
TTAAACAAGG TGCTGGGCAT TGTGCTGGGA ACGCTGATTA AGGACCATGA GATGCGTGGC 
GTTAGCTTCC AGCAGGTTGG CTACCATCGC TTCTTCATGA TGCTGTTCAT GGAGCTGTGC 
ACGGCCGATG TGATTCTCGA GTCCCTGATG CACAGTATTG TGTCGGCCTT CGCCTACACA 
TATCACTTGT TGAATCCCAG CGTGGCTCCG GGATTCTGCT TTGCTTGGTT GGAGCTCATT 
TCGCATCGCG TCTTCCTGGG CCGCATCCTG GTCCAAATTC CCGGGCAAAA GGGCTGGCCA 
CTTTACGCTC AGTTGCTGCA GGACCTTTTT AAATATCTTG CGCCCTTCTT ACGCAACACT 
GAGCTCGGCA AACCAGTCCA GCTTTTGTAC AAGGGGACGC TACGTGTTCT GCTCGTTCTG 
CTTCACGACT TCCCGGAGTT CTTGTGCGAC TATCATTTTG GGTTCTGTGA CACCATCCCG 
CCCAATTGCG TCCAGATGCG TAACATCATC CTGTCGGCGT TTCCGCGCAA CATGCGCTTG 
CCCGATCCCT TCACGCCCAA CCTAAAAGTG GACATGCTGT CGGACAGTAG TAATGCGCCT 
AAGGTGCTCA GTAGCTACAT TATGAACATC CAGCCCGCGA ACTTTAAGAA GGATTTGGAC 
TCGTACCTCA AGGCCCGGGC GCCAGTTACT TTTCTATCGG AATTACGTGG TCACTTGCAG 
GTGACAAGCG AGCCAGGAAC TCGTTACAAC ATGGCACTGA TGAATGCTCT GGTCATGTAT 
GTGGGAACTC AGGCGATTGC TTTGATCAGA AACAAAAACT TTGTGCCCAA CACCTCGAAC 
ATAGCACACA GCGCCCACAT GGATATCTTC CAGAATCTTG CTGTTGACTT GGATACGGAG 
GGTCGCTATC TGTTCTTGAA TGCGATTGCG AACCAGTTGC GCTATCCCAA CAGCCACACG 
CACTACTTCA GCTGTGCGGT GCTGCATCTG TTTGCCGAGG CCAACTCGGA GGCAATTCAG 
GAGCAGATCA CTCGCGTACT TCTGGAACGA TTGATCGTGA ACCGCCCGCA TCCTTGGGGA 
CTGTTGATCA CTTTTATTGA GCTGATCAAA AATCCCATCT ACAAGTTCTG GGACCATGAC 
TTTGTGCACT GTGCGCCAGA GATCACCAAG CTCTTTGAAT CGGTGGCCCG TTCCTGTCTG 
GCCAAGTCGA ACGTCACCCA GCAGTTGAAC ATGCCCGTCG TCGATGGCGA GGGCCAGGAG 
GTAGCCACCA TCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Not1 ( NM_001103773.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097243.1 Drosophila melanogaster Not1, transcript variant D (Not1), mRNA.

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CloneID	OFa11877
Clone ID Related Accession (Same CDS sequence)	NM_001103773.3
Accession Version	NM_001103773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Not1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103773.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CTCATGCGAT 
ACAGATGATC CGCCGGGCTT ACAAGAGAAG ACGGAGTTTC TGCTTAAGGA TTGGGTTGCA 
CTGTACACGC AACAGAACCA GCAGTCAACG CGCGATGCAC GCAACTTCGG TGCATTTGTT 
CAGAAGATGA ACACATATGG TATCCTGAAA ACGGACGACT TGATCACCCG TTTTTTCCGC 
CAGGCCACAC ACATTTGCAC GGATGTGGTG TACAGAATGT TCGCCGAGCC GAGTTTACCA 
ATCAACCAGG CCAAAAACAA GATATTCCAG TGGATCGATG CGTTCGTCCA TCTGATTGCA 
ATGCTCGTGC GACACTCAGG CGAAGCAGGT AATCCAACCA CCAAGATCAA CCTGTTAAAC 
AAGGTGCTGG GCATTGTGCT GGGAACGCTG ATTAAGGACC ATGAGATGCG TGGCGTTAGC 
TTCCAGCAGG TTGGCTACCA TCGCTTCTTC ATGATGCTGT TCATGGAGCT GTGCACGGCC 
GATGTGATTC TCGAGTCCCT GATGCACAGT ATTGTGTCGG CCTTCGCCTA CACATATCAC 
TTGTTGAATC CCAGCGTGGC TCCGGGATTC TGCTTTGCTT GGTTGGAGCT CATTTCGCAT 
CGCGTCTTCC TGGGCCGCAT CCTGGTCCAA ATTCCCGGGC AAAAGGGCTG GCCACTTTAC 
GCTCAGTTGC TGCAGGACCT TTTTAAATAT CTTGCGCCCT TCTTACGCAA CACTGAGCTC 
GGCAAACCAG TCCAGCTTTT GTACAAGGGG ACGCTACGTG TTCTGCTCGT TCTGCTTCAC 
GACTTCCCGG AGTTCTTGTG CGACTATCAT TTTGGGTTCT GTGACACCAT CCCGCCCAAT 
TGCGTCCAGA TGCGTAACAT CATCCTGTCG GCGTTTCCGC GCAACATGCG CTTGCCCGAT 
CCCTTCACGC CCAACCTAAA AGTGGACATG CTGTCGGACA GTAGTAATGC GCCTAAGGTG 
CTCAGTAGCT ACATTATGAA CATCCAGCCC GCGAACTTTA AGAAGGATTT GGACTCGTAC 
CTCAAGGCCC GGGCGCCAGT TACTTTTCTA TCGGAATTAC GTGGTCACTT GCAGGTGACA 
AGCGAGCCAG GAACTCGTTA CAACATGGCA CTGATGAATG CTCTGGTCAT GTATGTGGGA 
ACTCAGGCGA TTGCTTTGAT CAGAAACAAA AACTTTGTGC CCAACACCTC GAACATAGCA 
CACAGCGCCC ACATGGATAT CTTCCAGAAT CTTGCTGTTG ACTTGGATAC GGAGGGTCGC 
TATCTGTTCT TGAATGCGAT TGCGAACCAG TTGCGCTATC CCAACAGCCA CACGCACTAC 
TTCAGCTGTG CGGTGCTGCA TCTGTTTGCC GAGGCCAACT CGGAGGCAAT TCAGGAGCAG 
ATCACTCGCG TACTTCTGGA ACGATTGATC GTGAACCGCC CGCATCCTTG GGGACTGTTG 
ATCACTTTTA TTGAGCTGAT CAAAAATCCC ATCTACAAGT TCTGGGACCA TGACTTTGTG 
CACTGTGCGC CAGAGATCAC CAAGCTCTTT GAATCGGTGG CCCGTTCCTG TCTGGCCAAG 
TCGAACGTCA CCCAGCAGTT GAACATGCCC GTCGTCGATG GCGAGGGCCA GGAGGTAGCC 
ACCATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097243.1
CDS	719..8230
Translation	MNVESQLKTPPTHIRNLVKHVNKRNFNESSEQINQFVKEHGLEADRSSLRHLFSVINFSDLVPSVTVQLQAKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKSLKPVAVADLVGQVARLTGINKVCECVFALALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCLSEYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEADALNEVGIEDIYDHLSEIIFTNQGKNNIMDTSWINLILEIGYEFTSSVEECKNHLCSRERERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFNQLIPTFLGNHPNSNVILASAWSSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPEDQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVPPPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGPGMDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAAAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKALLSGLNGMPSMAEIQAAAMQLASENVELVCAFIQKTSAEKAAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSIPGFQQMSDRDIALFVPKPTDLSQPNVFANDDSMVYGELASKMEAFMNTAIGVPTLQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNNSFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSSSDYSPFNGNDRYLSGASHYIHSGMHHSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQAKNKIFQWIDAFVHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEGQEVATIN

Target ORF information:

RefSeq Version	NM_001103773.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Not1, transcript variant D (Not1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103773.3

ORF Insert Sequence:

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CTCATGCGAT 
ACAGATGATC CGCCGGGCTT ACAAGAGAAG ACGGAGTTTC TGCTTAAGGA TTGGGTTGCA 
CTGTACACGC AACAGAACCA GCAGTCAACG CGCGATGCAC GCAACTTCGG TGCATTTGTT 
CAGAAGATGA ACACATATGG TATCCTGAAA ACGGACGACT TGATCACCCG TTTTTTCCGC 
CAGGCCACAC ACATTTGCAC GGATGTGGTG TACAGAATGT TCGCCGAGCC GAGTTTACCA 
ATCAACCAGG CCAAAAACAA GATATTCCAG TGGATCGATG CGTTCGTCCA TCTGATTGCA 
ATGCTCGTGC GACACTCAGG CGAAGCAGGT AATCCAACCA CCAAGATCAA CCTGTTAAAC 
AAGGTGCTGG GCATTGTGCT GGGAACGCTG ATTAAGGACC ATGAGATGCG TGGCGTTAGC 
TTCCAGCAGG TTGGCTACCA TCGCTTCTTC ATGATGCTGT TCATGGAGCT GTGCACGGCC 
GATGTGATTC TCGAGTCCCT GATGCACAGT ATTGTGTCGG CCTTCGCCTA CACATATCAC 
TTGTTGAATC CCAGCGTGGC TCCGGGATTC TGCTTTGCTT GGTTGGAGCT CATTTCGCAT 
CGCGTCTTCC TGGGCCGCAT CCTGGTCCAA ATTCCCGGGC AAAAGGGCTG GCCACTTTAC 
GCTCAGTTGC TGCAGGACCT TTTTAAATAT CTTGCGCCCT TCTTACGCAA CACTGAGCTC 
GGCAAACCAG TCCAGCTTTT GTACAAGGGG ACGCTACGTG TTCTGCTCGT TCTGCTTCAC 
GACTTCCCGG AGTTCTTGTG CGACTATCAT TTTGGGTTCT GTGACACCAT CCCGCCCAAT 
TGCGTCCAGA TGCGTAACAT CATCCTGTCG GCGTTTCCGC GCAACATGCG CTTGCCCGAT 
CCCTTCACGC CCAACCTAAA AGTGGACATG CTGTCGGACA GTAGTAATGC GCCTAAGGTG 
CTCAGTAGCT ACATTATGAA CATCCAGCCC GCGAACTTTA AGAAGGATTT GGACTCGTAC 
CTCAAGGCCC GGGCGCCAGT TACTTTTCTA TCGGAATTAC GTGGTCACTT GCAGGTGACA 
AGCGAGCCAG GAACTCGTTA CAACATGGCA CTGATGAATG CTCTGGTCAT GTATGTGGGA 
ACTCAGGCGA TTGCTTTGAT CAGAAACAAA AACTTTGTGC CCAACACCTC GAACATAGCA 
CACAGCGCCC ACATGGATAT CTTCCAGAAT CTTGCTGTTG ACTTGGATAC GGAGGGTCGC 
TATCTGTTCT TGAATGCGAT TGCGAACCAG TTGCGCTATC CCAACAGCCA CACGCACTAC 
TTCAGCTGTG CGGTGCTGCA TCTGTTTGCC GAGGCCAACT CGGAGGCAAT TCAGGAGCAG 
ATCACTCGCG TACTTCTGGA ACGATTGATC GTGAACCGCC CGCATCCTTG GGGACTGTTG 
ATCACTTTTA TTGAGCTGAT CAAAAATCCC ATCTACAAGT TCTGGGACCA TGACTTTGTG 
CACTGTGCGC CAGAGATCAC CAAGCTCTTT GAATCGGTGG CCCGTTCCTG TCTGGCCAAG 
TCGAACGTCA CCCAGCAGTT GAACATGCCC GTCGTCGATG GCGAGGGCCA GGAGGTAGCC 
ACCATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Not1 ( NM_001103774.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097244.1 Drosophila melanogaster Not1, transcript variant E (Not1), mRNA.

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CloneID	OFa11878
Clone ID Related Accession (Same CDS sequence)	NM_001103774.3
Accession Version	NM_001103774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Not1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103774.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACAT CTTGGATTAA TCTAATCCTT GAAATCGGTT ATGAATTTAC ATCGAGCGTT 
GAAGAGTGTA AGAATCATTT GTGTTCCCGC GAGCGAGAAC GCGCTGAGCT CCAGTCCAAA 
GATGTCGCCA AGATCGTGGG TCTTATGTGC CGACGGCACT CCTCCCTGCT CGATTGTAAT 
GTGAATCTAC CGACGCCGGC GAACTTCTGG CCTGGTCAGG GACAGGGCGG TGGCAGCAAC 
AGCAGTGGCT CTTCGCAGAC ACAAATCACT CCGCAGCAAC AGAACCCGGG CAGCAGTAAC 
AACAACGATG GCAGCGATGG CAATTCGTCC AGTGATAAGA AGGACAAGAA GGAGACGACG 
GAGGCCACGC AAACTTGGAA GCCAGATGTC TTTGTGCAGG CGCTCAAGGA GGTGGTGCCT 
CAGCTCAACT GGAAGGACGT GTGCATGGAA CTTGATCATC CCGAGTTCGT GCTGAAGGAT 
CGCATCGGTT TGGAGCTTTT GCTCACCATC CTTCGGCTGG CCACTGGCTC TAACATATTT 
CCACATCCAG AATGCATTTA CCGTCACTGG GCAAACACGG AAGGTCAGCT GTCGCTTATC 
GCGACAATGC TGAAGAACCC GGATCTCTTT TCCTTCGCCG ACTTTGTATT TAGCCAGCCA 
GCGTTGGATG TGCTTAAGAC GGCTCCGGAT GCGGACAATA AGGAGATCTC GTCCTGGAAG 
TCGCTTCACC TGGTGGAGGT GCTGCTCTCC ATTGCGGACA AGGGATACTA TACGCAGGTC 
CATGAGCTAT TCAAGTTCCC AGCGCAGAAC TGTCCCGATG TGCTATTTTT GGCCTTATTG 
AACACAAGTC CGCCTATGAC GCCACTGCGT CAGGATCTGT TTAACCAACT GATACCAACG 
TTCCTTGGCA ACCATCCCAA CTCGAACGTG ATCTTGGCCA GCGCCTGGAG CTCGAACAAC 
TTCCAACTTC GTTCCAATAT CATGAATGCT ATGTCCGAGT GGTATCTCCG AGGCAATGAG 
TTCGATCAGG TGAAACTGTC GCGCATCCTG GATCTTGCCC AGGATTTAAA AGCGCTATCA 
GCGCTGCTCA ACGCCCGTTC ATTTTTATTC ATTATCGACC TGGCTTGCCT TGCCTCTCGG 
CGCGAGTACC TGAAGCTGGA AAAGTGGCTT ACCGATAAAA TTCGCGAACA TGGAGAGCCT 
TTTATGCAGG CTATAATCAA GGTTCTGCAC CGCCGCTGCC CGCAGGTTAT CAACGCAAAA 
GTGCCAGAGG ATCAGCTGCC TCCCAAGCAG GCGCAGCTTT TGCCCGAGAC AGTTACCACC 
ATGATCAACT GTCTGCAAAC GTGCATCAAT AACTGCATGC AGCCGGAGAT GGTCGAAGTC 
ATCATGCAAA TGACCGCGAA CGTGGCGATT ATGGCTAACA AGGCGCGTGC CCAACAGCAG 
CAGCAGCCAG GATTGGTTCC GCCGCCTCCA CCAACAATTC TGCGCGGCCA TCGTGGTATG 
GATCTGCCTG GAGGCATAGT CCCTCCGCCA CCGCAGCAAC CGTTCTCCGG AAACCTCAAC 
GCGCAGATGT TTGGACCAGG AATGGATCCG CTAACGAATA TGTCCAACAA CTTAGCCGGC 
CTCAACCTCA GCGGCCCGAA CGGAGCATTT AATTTTGGAA ACATGCTAAC TTCTCCATCG 
CGTCTGATGA CTCCGGGAGC CAATCCCTAT CCGCTGAACC TCATGCAGAT GCCGCAGGCT 
CCGCCACCAC CCAACGTAGG AAACCTAGGC CGCATGCTGC CAGGGGGTCC ACAACAGCAG 
ACTCCAACGC CGACTCCAAC GGCCCCCAAT CCAAACAACC CTGTGATGGC CGATCTCCAG 
ATACCTGTGT CAAAGGAAGT TGAAGACGAG GTCAACTCAT ACTTTCAGCG CATCTACAAC 
CACCAGCCGA ATCCAACGCT GTCCATCGAT GAAGTGCTGG ACATTTTGCA AAGATTTAAA 
GAGTCAAGTA ACCGGCGCGA GCAGGAAGTC TTTTTGTGCA TGCTGCGCAA TCTGTTCGAG 
GAATATCGCT TTTTCTGTCA ATACCCAGAG AAAGAGCTAC AGATTACTGC GCAGCTATTT 
GGTGGCATTA TTGATCGTAA CCTGGTGCCC ACTTTTGTTG CACTGGGCCT TTCTCTGCGC 
TGTGTCTTGG ATGCCCTCCG CAAGCCCGAT GGATCTAAGC TTTACTATTT CGGTGTGACG 
GCACTGGACA GATTCAGAAC TCGATTGCAC ACCTACAACA AATACTGCGA GCATATCCGC 
TCCATTCCTC ACTTCTCTGA CTTCCCGCCG CACCTTATTC AGTATGTGGA ATACGGAATG 
CACGGCCAGG AGCCGCCGCC GCAGAAGTTG ATTGGACTCA GCAATACGAT TCCTTCGGCG 
ATATCTAGCG GACCAGGAAC GGAACCAATT TACAGGAATA GCTCCATGTT AGGTAATATG 
CCCGCTGCCA CCCCGGGATC GGGACCGAAA TCAAACGCTG CTGTATCGCA TGCCACGAGA 
ATGAAGTCCA TCGCCAATGC CACCAATATC GACACACTTT TGGTGGCCAA CCAGGAAGAG 
AAAGTGACTG TGCCACCAGA GCCTGTGCAG GACAAAACTG CCTTTATTTT CAACAACTTG 
AGCCAGCTGA ACATACCGCA GAAGTGCGAT GAGATAAAGG AGATTATGAC CAAGGAGTAT 
TGGCCGTGGC TAGCGCAATA TCTGGTCCTC AAACGCGCAT CAATGGAGTT CAACTTCCAC 
ACACTATATT ACAACTTTTT GGATGCCCTT AAAAACGGCG AGATCAACCG ATTCGTAACC 
AAAGAGACCC TACGCAACAT CAAGGTACTC TTGCGCTCCG ATAAGGGAGT TATTAATTTC 
TCCGATCGAA GTCTGTTGAA GAACCTCGGA CACTGGTTGG GCATGATGAC CTTAGGTCGC 
AATCGCCCCA TCCTGCAGTT GGATCTGGAT CTTAAATCCC TTTTGGCTGA GGCTTACCAC 
AAGGGCCAGC AGGAACTGCT TTTTGTGGTT CCTTTCGTAG CCAAGATCCT TGAGTCATCC 
GCCAAGTCTC GCATCTTCCG ATCGCCCAAT CCATGGACAA TGGGTATCAT GTACGTGCTT 
GCTGAGCTTC ACCAGGAGCC AGACCTTAAG CTGAACCTGA AGTTTGAAAT CGAAGTACTC 
TGTAAAACAC TTAATCTGGA GTTGGCCAAG TTGCGACAAG TGATCTACCT GAAGGATCCC 
AATCGAACTC ACCGGATTGA AGAGCAGATG TCGCAACCAA AACCGAAACA ACTTGAGCCA 
GTGGCTTCTG CGCCAGCTCT ACCGCGTGAA CAGCAATCCC CGGCACAACC TCCGCCACCA 
CCACAACAGC AGCAACCACC GCAGCAGCAG GTACCTCCAC CGCCATCCTC TGCGGACGTG 
GACGCTCAAA ATGCGGCCGC TATGATGATG GCAGCGGGCG GAGCTAATAG TACTCCTGGA 
TCGGTATCTT CGCCCAATCT ACCCACCGAT TCCAGCCAGG TGGCACTACC GCCACCAGAG 
CCGCGCTATT CATACGTGGA CGTAAACGTG AGCAACTTCC AGCTTATTGG TCAGCAGTTG 
GTACTGCCAC CCAATACTCC GTTCCTGCAC GCCAATCCCG GAATCAAACA CATGGTCGTC 
AACGCCGTGG AGCGCACGAT AACCGACTGG TTGCAGCCTA TTGTGGACCG AAGCATCCGC 
ATTGCCTGTG CCACCACCGA GCAGATCATC CGCAAGGACT TCGCCCTGGA TGCTGACGAG 
AACCGAATGC GCACTGCCGC TCATCAGATG GTACGAAACC TTGCCGCCGG CATGGCCATG 
ATTACCGGTA AGGATGAGAT AGCGCGTGCT ATTAGCCAGA ATCTGCACAA GGCCTTGCTG 
TCGGGTCTAA ATGGGATGCC CAGCATGGCT GAGATCCAAG CTGCTGCTAT GCAGTTAGCC 
AGCGAAAATG TTGAGCTAGT TTGCGCTTTC ATTCAGAAAA CATCTGCTGA AAAGGCAGCC 
GCAGAGATCG ATAGGCGTCT AAGCACCGAT TTCGAGACCA GAAAAATTGC TCGTGAAGAG 
GGCAACCGCT TTGTGGACGC CCAGATCCTC ACCTATCAAC AGGAGCGTCT TCCAGAAGCA 
GTGCGCATCA AGGTGGGCGC GGCTCCAGCT ACACTTTATG CTGTGTACTC GGAGTTCGCT 
AGAAGCATAC CTGGCTTCCA GCAGATGAGC GATCGCGATA TTGCCCTGTT TGTGCCCAAG 
CCGACGGATT TGTCTCAGCC AAATGTGTTT GCTAATGATG ATAGCATGGT ATACGGGGAA 
CTGGCTAGCA AAATGGAGGC CTTCATGAAC ACGGCAATCG GAGTGCCGAC ACTCCAGATC 
CAGGCTAGCA AGATGCACAT GCTACTCAAC GCCCTGATAG CGACGCGTCG ACTGCGTGAC 
CAAGAGTCTG CCTTTAATCT GCTGACCCGC GCGGTCGAGG GTTTGACCGA AGGATTGGTC 
AATATGCATG AAAACATGGA GCAAATGAAG ATGTACCAAA ACATCCATCT CCGTATCCTT 
GGCTTGCTGA ACAATAGTTT CGGTGCTCCA AACACAGAGC GAGCGGTAAC AAAGTGTTTC 
TTCGATATCC GAGAGGAGGT GCGATATAAC GTGGAGGCAG CTCGCGCTTT GATTACGTCG 
CACTTTGTCA ATCTGAATCA GTTTGACGGA ATGCTACGCG ACTGCATGGA CAACGGAAAC 
AATTATGTGG CCATATCGTT CGGTATCGCA CTGCTGGAGC GCCTCATCAT GGACGATCGA 
GTGATTAATA TTGTTTCGGA CAACGAATTC ATGGCAACTG TTGAGCTGCT AGGTAGGCTC 
ACTCAGCATC GCCATCGGTA TCCAGAATGC ATTGTGAATG CTATTGATAC GCTCTGGAGC 
GGAAACTTTA ACTCGAGCAG CGACTACAGC CCGTTCAACG GCAATGATCG TTACTTGTCG 
GGAGCCTCCC ATTACATCCA CTCCGGCATG CATCACGTAA GGTCATGCGA TACAGATGAT 
CCGCCGGGCT TACAAGAGAA GACGGAGTTT CTGCTTAAGG ATTGGGTTGC ACTGTACACG 
CAACAGAACC AGCAGTCAAC GCGCGATGCA CGCAACTTCG GTGCATTTGT TCAGAAGATG 
AACACATATG GTATCCTGAA AACGGACGAC TTGATCACCC GTTTTTTCCG CCAGGCCACA 
CACATTTGCA CGGATGTGGT GTACAGAATG TTCGCCGAGC CGAGTTTACC AATCAACCAG 
GCCAAAAACA AGATATTCCA GTGGATCGAT GCGTTCGTCC ATCTGATTGC AATGCTCGTG 
CGACACTCAG GCGAAGCAGG TAATCCAACC ACCAAGATCA ACCTGTTAAA CAAGGTGCTG 
GGCATTGTGC TGGGAACGCT GATTAAGGAC CATGAGATGC GTGGCGTTAG CTTCCAGCAG 
GTTGGCTACC ATCGCTTCTT CATGATGCTG TTCATGGAGC TGTGCACGGC CGATGTGATT 
CTCGAGTCCC TGATGCACAG TATTGTGTCG GCCTTCGCCT ACACATATCA CTTGTTGAAT 
CCCAGCGTGG CTCCGGGATT CTGCTTTGCT TGGTTGGAGC TCATTTCGCA TCGCGTCTTC 
CTGGGCCGCA TCCTGGTCCA AATTCCCGGG CAAAAGGGCT GGCCACTTTA CGCTCAGTTG 
CTGCAGGACC TTTTTAAATA TCTTGCGCCC TTCTTACGCA ACACTGAGCT CGGCAAACCA 
GTCCAGCTTT TGTACAAGGG GACGCTACGT GTTCTGCTCG TTCTGCTTCA CGACTTCCCG 
GAGTTCTTGT GCGACTATCA TTTTGGGTTC TGTGACACCA TCCCGCCCAA TTGCGTCCAG 
ATGCGTAACA TCATCCTGTC GGCGTTTCCG CGCAACATGC GCTTGCCCGA TCCCTTCACG 
CCCAACCTAA AAGTGGACAT GCTGTCGGAC AGTAGTAATG CGCCTAAGGT GCTCAGTAGC 
TACATTATGA ACATCCAGCC CGCGAACTTT AAGAAGGATT TGGACTCGTA CCTCAAGGCC 
CGGGCGCCAG TTACTTTTCT ATCGGAATTA CGTGGTCACT TGCAGGTGAC AAGCGAGCCA 
GGAACTCGTT ACAACATGGC ACTGATGAAT GCTCTGGTCA TGTATGTGGG AACTCAGGCG 
ATTGCTTTGA TCAGAAACAA AAACTTTGTG CCCAACACCT CGAACATAGC ACACAGCGCC 
CACATGGATA TCTTCCAGAA TCTTGCTGTT GACTTGGATA CGGAGGGTCG CTATCTGTTC 
TTGAATGCGA TTGCGAACCA GTTGCGCTAT CCCAACAGCC ACACGCACTA CTTCAGCTGT 
GCGGTGCTGC ATCTGTTTGC CGAGGCCAAC TCGGAGGCAA TTCAGGAGCA GATCACTCGC 
GTACTTCTGG AACGATTGAT CGTGAACCGC CCGCATCCTT GGGGACTGTT GATCACTTTT 
ATTGAGCTGA TCAAAAATCC CATCTACAAG TTCTGGGACC ATGACTTTGT GCACTGTGCG 
CCAGAGATCA CCAAGCTCTT TGAATCGGTG GCCCGTTCCT GTCTGGCCAA GTCGAACGTC 
ACCCAGCAGT TGAACATGCC CGTCGTCGAT GGCGAGGGCC AGGAGGTAGC CACCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097244.1
CDS	1075..7737
Translation	MDTSWINLILEIGYEFTSSVEECKNHLCSRERERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFNQLIPTFLGNHPNSNVILASAWSSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPEDQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVPPPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGPGMDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAAAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKALLSGLNGMPSMAEIQAAAMQLASENVELVCAFIQKTSAEKAAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSIPGFQQMSDRDIALFVPKPTDLSQPNVFANDDSMVYGELASKMEAFMNTAIGVPTLQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNNSFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSSSDYSPFNGNDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQAKNKIFQWIDAFVHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEGQEVATIN

Target ORF information:

RefSeq Version	NM_001103774.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Not1, transcript variant E (Not1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103774.3

ORF Insert Sequence:

ATGGACACAT CTTGGATTAA TCTAATCCTT GAAATCGGTT ATGAATTTAC ATCGAGCGTT 
GAAGAGTGTA AGAATCATTT GTGTTCCCGC GAGCGAGAAC GCGCTGAGCT CCAGTCCAAA 
GATGTCGCCA AGATCGTGGG TCTTATGTGC CGACGGCACT CCTCCCTGCT CGATTGTAAT 
GTGAATCTAC CGACGCCGGC GAACTTCTGG CCTGGTCAGG GACAGGGCGG TGGCAGCAAC 
AGCAGTGGCT CTTCGCAGAC ACAAATCACT CCGCAGCAAC AGAACCCGGG CAGCAGTAAC 
AACAACGATG GCAGCGATGG CAATTCGTCC AGTGATAAGA AGGACAAGAA GGAGACGACG 
GAGGCCACGC AAACTTGGAA GCCAGATGTC TTTGTGCAGG CGCTCAAGGA GGTGGTGCCT 
CAGCTCAACT GGAAGGACGT GTGCATGGAA CTTGATCATC CCGAGTTCGT GCTGAAGGAT 
CGCATCGGTT TGGAGCTTTT GCTCACCATC CTTCGGCTGG CCACTGGCTC TAACATATTT 
CCACATCCAG AATGCATTTA CCGTCACTGG GCAAACACGG AAGGTCAGCT GTCGCTTATC 
GCGACAATGC TGAAGAACCC GGATCTCTTT TCCTTCGCCG ACTTTGTATT TAGCCAGCCA 
GCGTTGGATG TGCTTAAGAC GGCTCCGGAT GCGGACAATA AGGAGATCTC GTCCTGGAAG 
TCGCTTCACC TGGTGGAGGT GCTGCTCTCC ATTGCGGACA AGGGATACTA TACGCAGGTC 
CATGAGCTAT TCAAGTTCCC AGCGCAGAAC TGTCCCGATG TGCTATTTTT GGCCTTATTG 
AACACAAGTC CGCCTATGAC GCCACTGCGT CAGGATCTGT TTAACCAACT GATACCAACG 
TTCCTTGGCA ACCATCCCAA CTCGAACGTG ATCTTGGCCA GCGCCTGGAG CTCGAACAAC 
TTCCAACTTC GTTCCAATAT CATGAATGCT ATGTCCGAGT GGTATCTCCG AGGCAATGAG 
TTCGATCAGG TGAAACTGTC GCGCATCCTG GATCTTGCCC AGGATTTAAA AGCGCTATCA 
GCGCTGCTCA ACGCCCGTTC ATTTTTATTC ATTATCGACC TGGCTTGCCT TGCCTCTCGG 
CGCGAGTACC TGAAGCTGGA AAAGTGGCTT ACCGATAAAA TTCGCGAACA TGGAGAGCCT 
TTTATGCAGG CTATAATCAA GGTTCTGCAC CGCCGCTGCC CGCAGGTTAT CAACGCAAAA 
GTGCCAGAGG ATCAGCTGCC TCCCAAGCAG GCGCAGCTTT TGCCCGAGAC AGTTACCACC 
ATGATCAACT GTCTGCAAAC GTGCATCAAT AACTGCATGC AGCCGGAGAT GGTCGAAGTC 
ATCATGCAAA TGACCGCGAA CGTGGCGATT ATGGCTAACA AGGCGCGTGC CCAACAGCAG 
CAGCAGCCAG GATTGGTTCC GCCGCCTCCA CCAACAATTC TGCGCGGCCA TCGTGGTATG 
GATCTGCCTG GAGGCATAGT CCCTCCGCCA CCGCAGCAAC CGTTCTCCGG AAACCTCAAC 
GCGCAGATGT TTGGACCAGG AATGGATCCG CTAACGAATA TGTCCAACAA CTTAGCCGGC 
CTCAACCTCA GCGGCCCGAA CGGAGCATTT AATTTTGGAA ACATGCTAAC TTCTCCATCG 
CGTCTGATGA CTCCGGGAGC CAATCCCTAT CCGCTGAACC TCATGCAGAT GCCGCAGGCT 
CCGCCACCAC CCAACGTAGG AAACCTAGGC CGCATGCTGC CAGGGGGTCC ACAACAGCAG 
ACTCCAACGC CGACTCCAAC GGCCCCCAAT CCAAACAACC CTGTGATGGC CGATCTCCAG 
ATACCTGTGT CAAAGGAAGT TGAAGACGAG GTCAACTCAT ACTTTCAGCG CATCTACAAC 
CACCAGCCGA ATCCAACGCT GTCCATCGAT GAAGTGCTGG ACATTTTGCA AAGATTTAAA 
GAGTCAAGTA ACCGGCGCGA GCAGGAAGTC TTTTTGTGCA TGCTGCGCAA TCTGTTCGAG 
GAATATCGCT TTTTCTGTCA ATACCCAGAG AAAGAGCTAC AGATTACTGC GCAGCTATTT 
GGTGGCATTA TTGATCGTAA CCTGGTGCCC ACTTTTGTTG CACTGGGCCT TTCTCTGCGC 
TGTGTCTTGG ATGCCCTCCG CAAGCCCGAT GGATCTAAGC TTTACTATTT CGGTGTGACG 
GCACTGGACA GATTCAGAAC TCGATTGCAC ACCTACAACA AATACTGCGA GCATATCCGC 
TCCATTCCTC ACTTCTCTGA CTTCCCGCCG CACCTTATTC AGTATGTGGA ATACGGAATG 
CACGGCCAGG AGCCGCCGCC GCAGAAGTTG ATTGGACTCA GCAATACGAT TCCTTCGGCG 
ATATCTAGCG GACCAGGAAC GGAACCAATT TACAGGAATA GCTCCATGTT AGGTAATATG 
CCCGCTGCCA CCCCGGGATC GGGACCGAAA TCAAACGCTG CTGTATCGCA TGCCACGAGA 
ATGAAGTCCA TCGCCAATGC CACCAATATC GACACACTTT TGGTGGCCAA CCAGGAAGAG 
AAAGTGACTG TGCCACCAGA GCCTGTGCAG GACAAAACTG CCTTTATTTT CAACAACTTG 
AGCCAGCTGA ACATACCGCA GAAGTGCGAT GAGATAAAGG AGATTATGAC CAAGGAGTAT 
TGGCCGTGGC TAGCGCAATA TCTGGTCCTC AAACGCGCAT CAATGGAGTT CAACTTCCAC 
ACACTATATT ACAACTTTTT GGATGCCCTT AAAAACGGCG AGATCAACCG ATTCGTAACC 
AAAGAGACCC TACGCAACAT CAAGGTACTC TTGCGCTCCG ATAAGGGAGT TATTAATTTC 
TCCGATCGAA GTCTGTTGAA GAACCTCGGA CACTGGTTGG GCATGATGAC CTTAGGTCGC 
AATCGCCCCA TCCTGCAGTT GGATCTGGAT CTTAAATCCC TTTTGGCTGA GGCTTACCAC 
AAGGGCCAGC AGGAACTGCT TTTTGTGGTT CCTTTCGTAG CCAAGATCCT TGAGTCATCC 
GCCAAGTCTC GCATCTTCCG ATCGCCCAAT CCATGGACAA TGGGTATCAT GTACGTGCTT 
GCTGAGCTTC ACCAGGAGCC AGACCTTAAG CTGAACCTGA AGTTTGAAAT CGAAGTACTC 
TGTAAAACAC TTAATCTGGA GTTGGCCAAG TTGCGACAAG TGATCTACCT GAAGGATCCC 
AATCGAACTC ACCGGATTGA AGAGCAGATG TCGCAACCAA AACCGAAACA ACTTGAGCCA 
GTGGCTTCTG CGCCAGCTCT ACCGCGTGAA CAGCAATCCC CGGCACAACC TCCGCCACCA 
CCACAACAGC AGCAACCACC GCAGCAGCAG GTACCTCCAC CGCCATCCTC TGCGGACGTG 
GACGCTCAAA ATGCGGCCGC TATGATGATG GCAGCGGGCG GAGCTAATAG TACTCCTGGA 
TCGGTATCTT CGCCCAATCT ACCCACCGAT TCCAGCCAGG TGGCACTACC GCCACCAGAG 
CCGCGCTATT CATACGTGGA CGTAAACGTG AGCAACTTCC AGCTTATTGG TCAGCAGTTG 
GTACTGCCAC CCAATACTCC GTTCCTGCAC GCCAATCCCG GAATCAAACA CATGGTCGTC 
AACGCCGTGG AGCGCACGAT AACCGACTGG TTGCAGCCTA TTGTGGACCG AAGCATCCGC 
ATTGCCTGTG CCACCACCGA GCAGATCATC CGCAAGGACT TCGCCCTGGA TGCTGACGAG 
AACCGAATGC GCACTGCCGC TCATCAGATG GTACGAAACC TTGCCGCCGG CATGGCCATG 
ATTACCGGTA AGGATGAGAT AGCGCGTGCT ATTAGCCAGA ATCTGCACAA GGCCTTGCTG 
TCGGGTCTAA ATGGGATGCC CAGCATGGCT GAGATCCAAG CTGCTGCTAT GCAGTTAGCC 
AGCGAAAATG TTGAGCTAGT TTGCGCTTTC ATTCAGAAAA CATCTGCTGA AAAGGCAGCC 
GCAGAGATCG ATAGGCGTCT AAGCACCGAT TTCGAGACCA GAAAAATTGC TCGTGAAGAG 
GGCAACCGCT TTGTGGACGC CCAGATCCTC ACCTATCAAC AGGAGCGTCT TCCAGAAGCA 
GTGCGCATCA AGGTGGGCGC GGCTCCAGCT ACACTTTATG CTGTGTACTC GGAGTTCGCT 
AGAAGCATAC CTGGCTTCCA GCAGATGAGC GATCGCGATA TTGCCCTGTT TGTGCCCAAG 
CCGACGGATT TGTCTCAGCC AAATGTGTTT GCTAATGATG ATAGCATGGT ATACGGGGAA 
CTGGCTAGCA AAATGGAGGC CTTCATGAAC ACGGCAATCG GAGTGCCGAC ACTCCAGATC 
CAGGCTAGCA AGATGCACAT GCTACTCAAC GCCCTGATAG CGACGCGTCG ACTGCGTGAC 
CAAGAGTCTG CCTTTAATCT GCTGACCCGC GCGGTCGAGG GTTTGACCGA AGGATTGGTC 
AATATGCATG AAAACATGGA GCAAATGAAG ATGTACCAAA ACATCCATCT CCGTATCCTT 
GGCTTGCTGA ACAATAGTTT CGGTGCTCCA AACACAGAGC GAGCGGTAAC AAAGTGTTTC 
TTCGATATCC GAGAGGAGGT GCGATATAAC GTGGAGGCAG CTCGCGCTTT GATTACGTCG 
CACTTTGTCA ATCTGAATCA GTTTGACGGA ATGCTACGCG ACTGCATGGA CAACGGAAAC 
AATTATGTGG CCATATCGTT CGGTATCGCA CTGCTGGAGC GCCTCATCAT GGACGATCGA 
GTGATTAATA TTGTTTCGGA CAACGAATTC ATGGCAACTG TTGAGCTGCT AGGTAGGCTC 
ACTCAGCATC GCCATCGGTA TCCAGAATGC ATTGTGAATG CTATTGATAC GCTCTGGAGC 
GGAAACTTTA ACTCGAGCAG CGACTACAGC CCGTTCAACG GCAATGATCG TTACTTGTCG 
GGAGCCTCCC ATTACATCCA CTCCGGCATG CATCACGTAA GGTCATGCGA TACAGATGAT 
CCGCCGGGCT TACAAGAGAA GACGGAGTTT CTGCTTAAGG ATTGGGTTGC ACTGTACACG 
CAACAGAACC AGCAGTCAAC GCGCGATGCA CGCAACTTCG GTGCATTTGT TCAGAAGATG 
AACACATATG GTATCCTGAA AACGGACGAC TTGATCACCC GTTTTTTCCG CCAGGCCACA 
CACATTTGCA CGGATGTGGT GTACAGAATG TTCGCCGAGC CGAGTTTACC AATCAACCAG 
GCCAAAAACA AGATATTCCA GTGGATCGAT GCGTTCGTCC ATCTGATTGC AATGCTCGTG 
CGACACTCAG GCGAAGCAGG TAATCCAACC ACCAAGATCA ACCTGTTAAA CAAGGTGCTG 
GGCATTGTGC TGGGAACGCT GATTAAGGAC CATGAGATGC GTGGCGTTAG CTTCCAGCAG 
GTTGGCTACC ATCGCTTCTT CATGATGCTG TTCATGGAGC TGTGCACGGC CGATGTGATT 
CTCGAGTCCC TGATGCACAG TATTGTGTCG GCCTTCGCCT ACACATATCA CTTGTTGAAT 
CCCAGCGTGG CTCCGGGATT CTGCTTTGCT TGGTTGGAGC TCATTTCGCA TCGCGTCTTC 
CTGGGCCGCA TCCTGGTCCA AATTCCCGGG CAAAAGGGCT GGCCACTTTA CGCTCAGTTG 
CTGCAGGACC TTTTTAAATA TCTTGCGCCC TTCTTACGCA ACACTGAGCT CGGCAAACCA 
GTCCAGCTTT TGTACAAGGG GACGCTACGT GTTCTGCTCG TTCTGCTTCA CGACTTCCCG 
GAGTTCTTGT GCGACTATCA TTTTGGGTTC TGTGACACCA TCCCGCCCAA TTGCGTCCAG 
ATGCGTAACA TCATCCTGTC GGCGTTTCCG CGCAACATGC GCTTGCCCGA TCCCTTCACG 
CCCAACCTAA AAGTGGACAT GCTGTCGGAC AGTAGTAATG CGCCTAAGGT GCTCAGTAGC 
TACATTATGA ACATCCAGCC CGCGAACTTT AAGAAGGATT TGGACTCGTA CCTCAAGGCC 
CGGGCGCCAG TTACTTTTCT ATCGGAATTA CGTGGTCACT TGCAGGTGAC AAGCGAGCCA 
GGAACTCGTT ACAACATGGC ACTGATGAAT GCTCTGGTCA TGTATGTGGG AACTCAGGCG 
ATTGCTTTGA TCAGAAACAA AAACTTTGTG CCCAACACCT CGAACATAGC ACACAGCGCC 
CACATGGATA TCTTCCAGAA TCTTGCTGTT GACTTGGATA CGGAGGGTCG CTATCTGTTC 
TTGAATGCGA TTGCGAACCA GTTGCGCTAT CCCAACAGCC ACACGCACTA CTTCAGCTGT 
GCGGTGCTGC ATCTGTTTGC CGAGGCCAAC TCGGAGGCAA TTCAGGAGCA GATCACTCGC 
GTACTTCTGG AACGATTGAT CGTGAACCGC CCGCATCCTT GGGGACTGTT GATCACTTTT 
ATTGAGCTGA TCAAAAATCC CATCTACAAG TTCTGGGACC ATGACTTTGT GCACTGTGCG 
CCAGAGATCA CCAAGCTCTT TGAATCGGTG GCCCGTTCCT GTCTGGCCAA GTCGAACGTC 
ACCCAGCAGT TGAACATGCC CGTCGTCGAT GGCGAGGGCC AGGAGGTAGC CACCATCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Not1 ( NM_001169628.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163099.1 Drosophila melanogaster Not1, transcript variant F (Not1), mRNA.

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CloneID	OFa12480
Clone ID Related Accession (Same CDS sequence)	NM_001169628.2
Accession Version	NM_001169628.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Not1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169628.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CGTAAGGTCA 
TGCGATACAG ATGATCCGCC GGGCTTACAA GAGAAGACGG AGTTTCTGCT TAAGGATTGG 
GTTGCACTGT ACACGCAACA GAACCAGCAG TCAACGCGCG ATGCACGCAA CTTCGGTGCA 
TTTGTTCAGA AGATGAACAC ATATGGTATC CTGAAAACGG ACGACTTGAT CACCCGTTTT 
TTCCGCCAGG CCACACACAT TTGCACGGAT GTGGTGTACA GAATGTTCGC CGAGCCGAGT 
TTACCAATCA ACCAGGCCAA AAACAAGATA TTCCAGTGGA TCGATGCGTT CGTCCATCTG 
ATTGCAATGC TCGTGCGACA CTCAGGCGAA GCAGGTAATC CAACCACCAA GATCAACCTG 
TTAAACAAGG TGCTGGGCAT TGTGCTGGGA ACGCTGATTA AGGACCATGA GATGCGTGGC 
GTTAGCTTCC AGCAGGTTGG CTACCATCGC TTCTTCATGA TGCTGTTCAT GGAGCTGTGC 
ACGGCCGATG TGATTCTCGA GTCCCTGATG CACAGTATTG TGTCGGCCTT CGCCTACACA 
TATCACTTGT TGAATCCCAG CGTGGCTCCG GGATTCTGCT TTGCTTGGTT GGAGCTCATT 
TCGCATCGCG TCTTCCTGGG CCGCATCCTG GTCCAAATTC CCGGGCAAAA GGGCTGGCCA 
CTTTACGCTC AGTTGCTGCA GGACCTTTTT AAATATCTTG CGCCCTTCTT ACGCAACACT 
GAGCTCGGCA AACCAGTCCA GCTTTTGTAC AAGGGGACGC TACGTGTTCT GCTCGTTCTG 
CTTCACGACT TCCCGGAGTT CTTGTGCGAC TATCATTTTG GGTTCTGTGA CACCATCCCG 
CCCAATTGCG TCCAGATGCG TAACATCATC CTGTCGGCGT TTCCGCGCAA CATGCGCTTG 
CCCGATCCCT TCACGCCCAA CCTAAAAGTG GACATGCTGT CGGACAGTAG TAATGCGCCT 
AAGGTGCTCA GTAGCTACAT TATGAACATC CAGCCCGCGA ACTTTAAGAA GGATTTGGAC 
TCGTACCTCA AGGCCCGGGC GCCAGTTACT TTTCTATCGG AATTACGTGG TCACTTGCAG 
GTGACAAGCG AGCCAGGAAC TCGTTACAAC ATGGCACTGA TGAATGCTCT GGTCATGTAT 
GTGGGAACTC AGGCGATTGC TTTGATCAGA AACAAAAACT TTGTGCCCAA CACCTCGAAC 
ATAGCACACA GCGCCCACAT GGATATCTTC CAGAATCTTG CTGTTGACTT GGATACGGAG 
GGTCGCTATC TGTTCTTGAA TGCGATTGCG AACCAGTTGC GCTATCCCAA CAGCCACACG 
CACTACTTCA GCTGTGCGGT GCTGCATCTG TTTGCCGAGG CCAACTCGGA GGCAATTCAG 
GAGCAGATCA CTCGCGTACT TCTGGAACGA TTGATCGTGA ACCGCCCGCA TCCTTGGGGA 
CTGTTGATCA CTTTTATTGA GCTGATCAAA AATCCCATCT ACAAGTTCTG GGACCATGAC 
TTTGTGCACT GTGCGCCAGA GATCACCAAA TATTTCGGTG AATGTTTGAT TGTAGTTAAG 
CGGTTTGAGA ATTCAATTGG CATTCAGTGT GCATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163099.1
CDS	719..8197
Translation	MNVESQLKTPPTHIRNLVKHVNKRNFNESSEQINQFVKEHGLEADRSSLRHLFSVINFSDLVPSVTVQLQAKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKSLKPVAVADLVGQVARLTGINKVCECVFALALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCLSEYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEADALNEVGIEDIYDHLSEIIFTNQGKNNIMDTSWINLILEIGYEFTSSVEECKNHLCSRERERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFNQLIPTFLGNHPNSNVILASAWSSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPEDQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVPPPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGPGMDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAAAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKALLSGLNGMPSMAEIQAAAMQLASENVELVCAFIQKTSAEKAAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSIPGFQQMSDRDIALFVPKPTDLSQPNVFANDDSMVYGELASKMEAFMNTAIGVPTLQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNNSFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSSSDYSPFNGNDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQAKNKIFQWIDAFVHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKYFGECLIVVKRFENSIGIQCAS

Target ORF information:

RefSeq Version	NM_001169628.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Not1, transcript variant F (Not1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169628.2

ORF Insert Sequence:

ATGAACGTAG AGAGCCAACT GAAGACACCG CCAACGCACA TTCGAAATCT GGTCAAGCAC 
GTGAACAAGC GAAATTTTAA TGAGAGCAGC GAGCAAATAA ATCAGTTCGT TAAAGAGCAC 
GGCCTGGAAG CGGATCGCAG CAGCCTGCGC CACCTGTTCT CCGTGATCAA CTTCAGCGAT 
CTCGTGCCAT CAGTTACGGT CCAGCTGCAG GCGAAGCTCC TGGGCATCCA GTTGGAGCGT 
CAGCTTCACA GTTCATCGTT TGTGTCCAAC ATCTGCTACG CTTTTGACCA GTTCTTCGCC 
AGCAACCAGA AGTCCCTGAA GCCTGTTGCG GTAGCGGATC TCGTTGGCCA GGTCGCCCGA 
CTGACCGGCA TCAACAAGGT TTGTGAGTGC GTCTTTGCCT TGGCCTTGAC CCACTCCTCG 
TACACAGAAC TCAAACATTC TGCCAGGAAC AACCTAAAAG GTAGCCTCAG CGAACTTATA 
GACTCTTACT TGGGTAATAA AGGCACCGGC CCAGCCGACA GCGGTCTCCG GGAGATCTCC 
TTCGATCTAC TGCAGTATTT GTTGTGCTGC CTTAGCGAGT ACGTACAACC GCAGGTGGAA 
GCACAGTTTT TGATCAAGCT TCGTGAGGAG TTTCCGCGCC AGGCGGTACC GCTGGTTCTA 
GCGCCGTTCC TTTACGGCTC GACGACGGCG ACGATTGCGG GAGCAGGTGC CAGCGAAACG 
GATGCGGAGG CCGAAGCGAC GACTAATAGC AACAGCTCCA GTTTCGAAGC AGATGCTTTG 
AACGAGGTGG GAATCGAGGA TATTTATGAC CATTTAAGCG AGATAATATT CACCAACCAG 
GGCAAAAATA ATATTATGGA CACATCTTGG ATTAATCTAA TCCTTGAAAT CGGTTATGAA 
TTTACATCGA GCGTTGAAGA GTGTAAGAAT CATTTGTGTT CCCGCGAGCG AGAACGCGCT 
GAGCTCCAGT CCAAAGATGT CGCCAAGATC GTGGGTCTTA TGTGCCGACG GCACTCCTCC 
CTGCTCGATT GTAATGTGAA TCTACCGACG CCGGCGAACT TCTGGCCTGG TCAGGGACAG 
GGCGGTGGCA GCAACAGCAG TGGCTCTTCG CAGACACAAA TCACTCCGCA GCAACAGAAC 
CCGGGCAGCA GTAACAACAA CGATGGCAGC GATGGCAATT CGTCCAGTGA TAAGAAGGAC 
AAGAAGGAGA CGACGGAGGC CACGCAAACT TGGAAGCCAG ATGTCTTTGT GCAGGCGCTC 
AAGGAGGTGG TGCCTCAGCT CAACTGGAAG GACGTGTGCA TGGAACTTGA TCATCCCGAG 
TTCGTGCTGA AGGATCGCAT CGGTTTGGAG CTTTTGCTCA CCATCCTTCG GCTGGCCACT 
GGCTCTAACA TATTTCCACA TCCAGAATGC ATTTACCGTC ACTGGGCAAA CACGGAAGGT 
CAGCTGTCGC TTATCGCGAC AATGCTGAAG AACCCGGATC TCTTTTCCTT CGCCGACTTT 
GTATTTAGCC AGCCAGCGTT GGATGTGCTT AAGACGGCTC CGGATGCGGA CAATAAGGAG 
ATCTCGTCCT GGAAGTCGCT TCACCTGGTG GAGGTGCTGC TCTCCATTGC GGACAAGGGA 
TACTATACGC AGGTCCATGA GCTATTCAAG TTCCCAGCGC AGAACTGTCC CGATGTGCTA 
TTTTTGGCCT TATTGAACAC AAGTCCGCCT ATGACGCCAC TGCGTCAGGA TCTGTTTAAC 
CAACTGATAC CAACGTTCCT TGGCAACCAT CCCAACTCGA ACGTGATCTT GGCCAGCGCC 
TGGAGCTCGA ACAACTTCCA ACTTCGTTCC AATATCATGA ATGCTATGTC CGAGTGGTAT 
CTCCGAGGCA ATGAGTTCGA TCAGGTGAAA CTGTCGCGCA TCCTGGATCT TGCCCAGGAT 
TTAAAAGCGC TATCAGCGCT GCTCAACGCC CGTTCATTTT TATTCATTAT CGACCTGGCT 
TGCCTTGCCT CTCGGCGCGA GTACCTGAAG CTGGAAAAGT GGCTTACCGA TAAAATTCGC 
GAACATGGAG AGCCTTTTAT GCAGGCTATA ATCAAGGTTC TGCACCGCCG CTGCCCGCAG 
GTTATCAACG CAAAAGTGCC AGAGGATCAG CTGCCTCCCA AGCAGGCGCA GCTTTTGCCC 
GAGACAGTTA CCACCATGAT CAACTGTCTG CAAACGTGCA TCAATAACTG CATGCAGCCG 
GAGATGGTCG AAGTCATCAT GCAAATGACC GCGAACGTGG CGATTATGGC TAACAAGGCG 
CGTGCCCAAC AGCAGCAGCA GCCAGGATTG GTTCCGCCGC CTCCACCAAC AATTCTGCGC 
GGCCATCGTG GTATGGATCT GCCTGGAGGC ATAGTCCCTC CGCCACCGCA GCAACCGTTC 
TCCGGAAACC TCAACGCGCA GATGTTTGGA CCAGGAATGG ATCCGCTAAC GAATATGTCC 
AACAACTTAG CCGGCCTCAA CCTCAGCGGC CCGAACGGAG CATTTAATTT TGGAAACATG 
CTAACTTCTC CATCGCGTCT GATGACTCCG GGAGCCAATC CCTATCCGCT GAACCTCATG 
CAGATGCCGC AGGCTCCGCC ACCACCCAAC GTAGGAAACC TAGGCCGCAT GCTGCCAGGG 
GGTCCACAAC AGCAGACTCC AACGCCGACT CCAACGGCCC CCAATCCAAA CAACCCTGTG 
ATGGCCGATC TCCAGATACC TGTGTCAAAG GAAGTTGAAG ACGAGGTCAA CTCATACTTT 
CAGCGCATCT ACAACCACCA GCCGAATCCA ACGCTGTCCA TCGATGAAGT GCTGGACATT 
TTGCAAAGAT TTAAAGAGTC AAGTAACCGG CGCGAGCAGG AAGTCTTTTT GTGCATGCTG 
CGCAATCTGT TCGAGGAATA TCGCTTTTTC TGTCAATACC CAGAGAAAGA GCTACAGATT 
ACTGCGCAGC TATTTGGTGG CATTATTGAT CGTAACCTGG TGCCCACTTT TGTTGCACTG 
GGCCTTTCTC TGCGCTGTGT CTTGGATGCC CTCCGCAAGC CCGATGGATC TAAGCTTTAC 
TATTTCGGTG TGACGGCACT GGACAGATTC AGAACTCGAT TGCACACCTA CAACAAATAC 
TGCGAGCATA TCCGCTCCAT TCCTCACTTC TCTGACTTCC CGCCGCACCT TATTCAGTAT 
GTGGAATACG GAATGCACGG CCAGGAGCCG CCGCCGCAGA AGTTGATTGG ACTCAGCAAT 
ACGATTCCTT CGGCGATATC TAGCGGACCA GGAACGGAAC CAATTTACAG GAATAGCTCC 
ATGTTAGGTA ATATGCCCGC TGCCACCCCG GGATCGGGAC CGAAATCAAA CGCTGCTGTA 
TCGCATGCCA CGAGAATGAA GTCCATCGCC AATGCCACCA ATATCGACAC ACTTTTGGTG 
GCCAACCAGG AAGAGAAAGT GACTGTGCCA CCAGAGCCTG TGCAGGACAA AACTGCCTTT 
ATTTTCAACA ACTTGAGCCA GCTGAACATA CCGCAGAAGT GCGATGAGAT AAAGGAGATT 
ATGACCAAGG AGTATTGGCC GTGGCTAGCG CAATATCTGG TCCTCAAACG CGCATCAATG 
GAGTTCAACT TCCACACACT ATATTACAAC TTTTTGGATG CCCTTAAAAA CGGCGAGATC 
AACCGATTCG TAACCAAAGA GACCCTACGC AACATCAAGG TACTCTTGCG CTCCGATAAG 
GGAGTTATTA ATTTCTCCGA TCGAAGTCTG TTGAAGAACC TCGGACACTG GTTGGGCATG 
ATGACCTTAG GTCGCAATCG CCCCATCCTG CAGTTGGATC TGGATCTTAA ATCCCTTTTG 
GCTGAGGCTT ACCACAAGGG CCAGCAGGAA CTGCTTTTTG TGGTTCCTTT CGTAGCCAAG 
ATCCTTGAGT CATCCGCCAA GTCTCGCATC TTCCGATCGC CCAATCCATG GACAATGGGT 
ATCATGTACG TGCTTGCTGA GCTTCACCAG GAGCCAGACC TTAAGCTGAA CCTGAAGTTT 
GAAATCGAAG TACTCTGTAA AACACTTAAT CTGGAGTTGG CCAAGTTGCG ACAAGTGATC 
TACCTGAAGG ATCCCAATCG AACTCACCGG ATTGAAGAGC AGATGTCGCA ACCAAAACCG 
AAACAACTTG AGCCAGTGGC TTCTGCGCCA GCTCTACCGC GTGAACAGCA ATCCCCGGCA 
CAACCTCCGC CACCACCACA ACAGCAGCAA CCACCGCAGC AGCAGGTACC TCCACCGCCA 
TCCTCTGCGG ACGTGGACGC TCAAAATGCG GCCGCTATGA TGATGGCAGC GGGCGGAGCT 
AATAGTACTC CTGGATCGGT ATCTTCGCCC AATCTACCCA CCGATTCCAG CCAGGTGGCA 
CTACCGCCAC CAGAGCCGCG CTATTCATAC GTGGACGTAA ACGTGAGCAA CTTCCAGCTT 
ATTGGTCAGC AGTTGGTACT GCCACCCAAT ACTCCGTTCC TGCACGCCAA TCCCGGAATC 
AAACACATGG TCGTCAACGC CGTGGAGCGC ACGATAACCG ACTGGTTGCA GCCTATTGTG 
GACCGAAGCA TCCGCATTGC CTGTGCCACC ACCGAGCAGA TCATCCGCAA GGACTTCGCC 
CTGGATGCTG ACGAGAACCG AATGCGCACT GCCGCTCATC AGATGGTACG AAACCTTGCC 
GCCGGCATGG CCATGATTAC CGGTAAGGAT GAGATAGCGC GTGCTATTAG CCAGAATCTG 
CACAAGGCCT TGCTGTCGGG TCTAAATGGG ATGCCCAGCA TGGCTGAGAT CCAAGCTGCT 
GCTATGCAGT TAGCCAGCGA AAATGTTGAG CTAGTTTGCG CTTTCATTCA GAAAACATCT 
GCTGAAAAGG CAGCCGCAGA GATCGATAGG CGTCTAAGCA CCGATTTCGA GACCAGAAAA 
ATTGCTCGTG AAGAGGGCAA CCGCTTTGTG GACGCCCAGA TCCTCACCTA TCAACAGGAG 
CGTCTTCCAG AAGCAGTGCG CATCAAGGTG GGCGCGGCTC CAGCTACACT TTATGCTGTG 
TACTCGGAGT TCGCTAGAAG CATACCTGGC TTCCAGCAGA TGAGCGATCG CGATATTGCC 
CTGTTTGTGC CCAAGCCGAC GGATTTGTCT CAGCCAAATG TGTTTGCTAA TGATGATAGC 
ATGGTATACG GGGAACTGGC TAGCAAAATG GAGGCCTTCA TGAACACGGC AATCGGAGTG 
CCGACACTCC AGATCCAGGC TAGCAAGATG CACATGCTAC TCAACGCCCT GATAGCGACG 
CGTCGACTGC GTGACCAAGA GTCTGCCTTT AATCTGCTGA CCCGCGCGGT CGAGGGTTTG 
ACCGAAGGAT TGGTCAATAT GCATGAAAAC ATGGAGCAAA TGAAGATGTA CCAAAACATC 
CATCTCCGTA TCCTTGGCTT GCTGAACAAT AGTTTCGGTG CTCCAAACAC AGAGCGAGCG 
GTAACAAAGT GTTTCTTCGA TATCCGAGAG GAGGTGCGAT ATAACGTGGA GGCAGCTCGC 
GCTTTGATTA CGTCGCACTT TGTCAATCTG AATCAGTTTG ACGGAATGCT ACGCGACTGC 
ATGGACAACG GAAACAATTA TGTGGCCATA TCGTTCGGTA TCGCACTGCT GGAGCGCCTC 
ATCATGGACG ATCGAGTGAT TAATATTGTT TCGGACAACG AATTCATGGC AACTGTTGAG 
CTGCTAGGTA GGCTCACTCA GCATCGCCAT CGGTATCCAG AATGCATTGT GAATGCTATT 
GATACGCTCT GGAGCGGAAA CTTTAACTCG AGCAGCGACT ACAGCCCGTT CAACGGCAAT 
GATCGTTACT TGTCGGGAGC CTCCCATTAC ATCCACTCCG GCATGCATCA CGTAAGGTCA 
TGCGATACAG ATGATCCGCC GGGCTTACAA GAGAAGACGG AGTTTCTGCT TAAGGATTGG 
GTTGCACTGT ACACGCAACA GAACCAGCAG TCAACGCGCG ATGCACGCAA CTTCGGTGCA 
TTTGTTCAGA AGATGAACAC ATATGGTATC CTGAAAACGG ACGACTTGAT CACCCGTTTT 
TTCCGCCAGG CCACACACAT TTGCACGGAT GTGGTGTACA GAATGTTCGC CGAGCCGAGT 
TTACCAATCA ACCAGGCCAA AAACAAGATA TTCCAGTGGA TCGATGCGTT CGTCCATCTG 
ATTGCAATGC TCGTGCGACA CTCAGGCGAA GCAGGTAATC CAACCACCAA GATCAACCTG 
TTAAACAAGG TGCTGGGCAT TGTGCTGGGA ACGCTGATTA AGGACCATGA GATGCGTGGC 
GTTAGCTTCC AGCAGGTTGG CTACCATCGC TTCTTCATGA TGCTGTTCAT GGAGCTGTGC 
ACGGCCGATG TGATTCTCGA GTCCCTGATG CACAGTATTG TGTCGGCCTT CGCCTACACA 
TATCACTTGT TGAATCCCAG CGTGGCTCCG GGATTCTGCT TTGCTTGGTT GGAGCTCATT 
TCGCATCGCG TCTTCCTGGG CCGCATCCTG GTCCAAATTC CCGGGCAAAA GGGCTGGCCA 
CTTTACGCTC AGTTGCTGCA GGACCTTTTT AAATATCTTG CGCCCTTCTT ACGCAACACT 
GAGCTCGGCA AACCAGTCCA GCTTTTGTAC AAGGGGACGC TACGTGTTCT GCTCGTTCTG 
CTTCACGACT TCCCGGAGTT CTTGTGCGAC TATCATTTTG GGTTCTGTGA CACCATCCCG 
CCCAATTGCG TCCAGATGCG TAACATCATC CTGTCGGCGT TTCCGCGCAA CATGCGCTTG 
CCCGATCCCT TCACGCCCAA CCTAAAAGTG GACATGCTGT CGGACAGTAG TAATGCGCCT 
AAGGTGCTCA GTAGCTACAT TATGAACATC CAGCCCGCGA ACTTTAAGAA GGATTTGGAC 
TCGTACCTCA AGGCCCGGGC GCCAGTTACT TTTCTATCGG AATTACGTGG TCACTTGCAG 
GTGACAAGCG AGCCAGGAAC TCGTTACAAC ATGGCACTGA TGAATGCTCT GGTCATGTAT 
GTGGGAACTC AGGCGATTGC TTTGATCAGA AACAAAAACT TTGTGCCCAA CACCTCGAAC 
ATAGCACACA GCGCCCACAT GGATATCTTC CAGAATCTTG CTGTTGACTT GGATACGGAG 
GGTCGCTATC TGTTCTTGAA TGCGATTGCG AACCAGTTGC GCTATCCCAA CAGCCACACG 
CACTACTTCA GCTGTGCGGT GCTGCATCTG TTTGCCGAGG CCAACTCGGA GGCAATTCAG 
GAGCAGATCA CTCGCGTACT TCTGGAACGA TTGATCGTGA ACCGCCCGCA TCCTTGGGGA 
CTGTTGATCA CTTTTATTGA GCTGATCAAA AATCCCATCT ACAAGTTCTG GGACCATGAC 
TTTGTGCACT GTGCGCCAGA GATCACCAAA TATTTCGGTG AATGTTTGAT TGTAGTTAAG 
CGGTTTGAGA ATTCAATTGG CATTCAGTGT GCATCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Not1 ( NM_001299323.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286252.1 Drosophila melanogaster Not1, transcript variant H (Not1), mRNA.

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CloneID	OFa22408
Clone ID Related Accession (Same CDS sequence)	NM_001299323.1
Accession Version	NM_001299323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Not1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGTCCCA GATTGCGGGC AGTCGCTGTC AAGGCACTTA TATTGGCTTC CAAACCCTAC 
GCAATGCAGC TCTTTGATTC TAAACCATAC ATTACTGATA GCATTTACGA CATCTTGCAG 
TTCGTTAAAG AGCACGGCCT GGAAGCGGAT CGCAGCAGCC TGCGCCACCT GTTCTCCGTG 
ATCAACTTCA GCGATCTCGT GCCATCAGTT ACGGTCCAGC TGCAGGCGAA GCTCCTGGGC 
ATCCAGTTGG AGCGTCAGCT TCACAGTTCA TCGTTTGTGT CCAACATCTG CTACGCTTTT 
GACCAGTTCT TCGCCAGCAA CCAGAAGCAG TCCCTGAAGC CTGTTGCGGT AGCGGATCTC 
GTTGGCCAGG TCGCCCGACT GACCGGCATC AACAAGGTTT GTGAGTGCGT CTTTGCCTTG 
GCCTTGACCC ACTCCTCGTA CACAGAACTC AAACATTCTG CCAGGAACAA CCTAAAAGGT 
AGCCTCAGCG AACTTATAGA CTCTTACTTG GGTAATAAAG GCACCGGCCC AGCCGACAGC 
GGTCTCCGGG AGATCTCCTT CGATCTACTG CAGTATTTGT TGTGCTGCCT TAGCGAGTAC 
GTACAACCGC AGGTGGAAGC ACAGTTTTTG ATCAAGCTTC GTGAGGAGTT TCCGCGCCAG 
GCGGTACCGC TGGTTCTAGC GCCGTTCCTT TACGGCTCGA CGACGGCGAC GATTGCGGGA 
GCAGGTGCCA GCGAAACGGA TGCGGAGGCC GAAGCGACGA CTAATAGCAA CAGCTCCAGT 
TTCGAAGCAG ATGCTTTGAA CGAGGTGGGA ATCGAGGATA TTTATGACCA TTTAAGCGAG 
ATAATATTCA CCAACCAGGG CAAAAATAAT ATTATGGACA CATCTTGGAT TAATCTAATC 
CTTGAAATCG GTTATGAATT TACATCGAGC GTTGAAGAGT GTAAGAATCA TTTGTGTTCC 
CGCGAGCGAG AACGCGCTGA GCTCCAGTCC AAAGATGTCG CCAAGATCGT GGGTCTTATG 
TGCCGACGGC ACTCCTCCCT GCTCGATTGT AATGTGAATC TACCGACGCC GGCGAACTTC 
TGGCCTGGTC AGGGACAGGG CGGTGGCAGC AACAGCAGTG GCTCTTCGCA GACACAAATC 
ACTCCGCAGC AACAGAACCC GGGCAGCAGT AACAACAACG ATGGCAGCGA TGGCAATTCG 
TCCAGTGATA AGAAGGACAA GAAGGAGACG ACGGAGGCCA CGCAAACTTG GAAGCCAGAT 
GTCTTTGTGC AGGCGCTCAA GGAGGTGGTG CCTCAGCTCA ACTGGAAGGA CGTGTGCATG 
GAACTTGATC ATCCCGAGTT CGTGCTGAAG GATCGCATCG GTTTGGAGCT TTTGCTCACC 
ATCCTTCGGC TGGCCACTGG CTCTAACATA TTTCCACATC CAGAATGCAT TTACCGTCAC 
TGGGCAAACA CGGAAGGTCA GCTGTCGCTT ATCGCGACAA TGCTGAAGAA CCCGGATCTC 
TTTTCCTTCG CCGACTTTGT ATTTAGCCAG CCAGCGTTGG ATGTGCTTAA GACGGCTCCG 
GATGCGGACA ATAAGGAGAT CTCGTCCTGG AAGTCGCTTC ACCTGGTGGA GGTGCTGCTC 
TCCATTGCGG ACAAGGGATA CTATACGCAG GTCCATGAGC TATTCAAGTT CCCAGCGCAG 
AACTGTCCCG ATGTGCTATT TTTGGCCTTA TTGAACACAA GTCCGCCTAT GACGCCACTG 
CGTCAGGATC TGTTTAACCA ACTGATACCA ACGTTCCTTG GCAACCATCC CAACTCGAAC 
GTGATCTTGG CCAGCGCCTG GAGCTCGAAC AACTTCCAAC TTCGTTCCAA TATCATGAAT 
GCTATGTCCG AGTGGTATCT CCGAGGCAAT GAGTTCGATC AGGTGAAACT GTCGCGCATC 
CTGGATCTTG CCCAGGATTT AAAAGCGCTA TCAGCGCTGC TCAACGCCCG TTCATTTTTA 
TTCATTATCG ACCTGGCTTG CCTTGCCTCT CGGCGCGAGT ACCTGAAGCT GGAAAAGTGG 
CTTACCGATA AAATTCGCGA ACATGGAGAG CCTTTTATGC AGGCTATAAT CAAGGTTCTG 
CACCGCCGCT GCCCGCAGGT TATCAACGCA AAAGTGCCAG AGGATCAGCT GCCTCCCAAG 
CAGGCGCAGC TTTTGCCCGA GACAGTTACC ACCATGATCA ACTGTCTGCA AACGTGCATC 
AATAACTGCA TGCAGCCGGA GATGGTCGAA GTCATCATGC AAATGACCGC GAACGTGGCG 
ATTATGGCTA ACAAGGCGCG TGCCCAACAG CAGCAGCAGC CAGGATTGGT TCCGCCGCCT 
CCACCAACAA TTCTGCGCGG CCATCGTGGT ATGGATCTGC CTGGAGGCAT AGTCCCTCCG 
CCACCGCAGC AACCGTTCTC CGGAAACCTC AACGCGCAGA TGTTTGGACC AGGAATGGAT 
CCGCTAACGA ATATGTCCAA CAACTTAGCC GGCCTCAACC TCAGCGGCCC GAACGGAGCA 
TTTAATTTTG GAAACATGCT AACTTCTCCA TCGCGTCTGA TGACTCCGGG AGCCAATCCC 
TATCCGCTGA ACCTCATGCA GATGCCGCAG GCTCCGCCAC CACCCAACGT AGGAAACCTA 
GGCCGCATGC TGCCAGGGGG TCCACAACAG CAGACTCCAA CGCCGACTCC AACGGCCCCC 
AATCCAAACA ACCCTGTGAT GGCCGATCTC CAGATACCTG TGTCAAAGGA AGTTGAAGAC 
GAGGTCAACT CATACTTTCA GCGCATCTAC AACCACCAGC CGAATCCAAC GCTGTCCATC 
GATGAAGTGC TGGACATTTT GCAAAGATTT AAAGAGTCAA GTAACCGGCG CGAGCAGGAA 
GTCTTTTTGT GCATGCTGCG CAATCTGTTC GAGGAATATC GCTTTTTCTG TCAATACCCA 
GAGAAAGAGC TACAGATTAC TGCGCAGCTA TTTGGTGGCA TTATTGATCG TAACCTGGTG 
CCCACTTTTG TTGCACTGGG CCTTTCTCTG CGCTGTGTCT TGGATGCCCT CCGCAAGCCC 
GATGGATCTA AGCTTTACTA TTTCGGTGTG ACGGCACTGG ACAGATTCAG AACTCGATTG 
CACACCTACA ACAAATACTG CGAGCATATC CGCTCCATTC CTCACTTCTC TGACTTCCCG 
CCGCACCTTA TTCAGTATGT GGAATACGGA ATGCACGGCC AGGAGCCGCC GCCGCAGAAG 
TTGATTGGAC TCAGCAATAC GATTCCTTCG GCGATATCTA GCGGACCAGG AACGGAACCA 
ATTTACAGGA ATAGCTCCAT GTTAGGTAAT ATGCCCGCTG CCACCCCGGG ATCGGGACCG 
AAATCAAACG CTGCTGTATC GCATGCCACG AGAATGAAGT CCATCGCCAA TGCCACCAAT 
ATCGACACAC TTTTGGTGGC CAACCAGGAA GAGAAAGTGA CTGTGCCACC AGAGCCTGTG 
CAGGACAAAA CTGCCTTTAT TTTCAACAAC TTGAGCCAGC TGAACATACC GCAGAAGTGC 
GATGAGATAA AGGAGATTAT GACCAAGGAG TATTGGCCGT GGCTAGCGCA ATATCTGGTC 
CTCAAACGCG CATCAATGGA GTTCAACTTC CACACACTAT ATTACAACTT TTTGGATGCC 
CTTAAAAACG GCGAGATCAA CCGATTCGTA ACCAAAGAGA CCCTACGCAA CATCAAGGTA 
CTCTTGCGCT CCGATAAGGG AGTTATTAAT TTCTCCGATC GAAGTCTGTT GAAGAACCTC 
GGACACTGGT TGGGCATGAT GACCTTAGGT CGCAATCGCC CCATCCTGCA GTTGGATCTG 
GATCTTAAAT CCCTTTTGGC TGAGGCTTAC CACAAGGGCC AGCAGGAACT GCTTTTTGTG 
GTTCCTTTCG TAGCCAAGAT CCTTGAGTCA TCCGCCAAGT CTCGCATCTT CCGATCGCCC 
AATCCATGGA CAATGGGTAT CATGTACGTG CTTGCTGAGC TTCACCAGGA GCCAGACCTT 
AAGCTGAACC TGAAGTTTGA AATCGAAGTA CTCTGTAAAA CACTTAATCT GGAGTTGGCC 
AAGTTGCGAC AAGTGATCTA CCTGAAGGAT CCCAATCGAA CTCACCGGAT TGAAGAGCAG 
ATGTCGCAAC CAAAACCGAA ACAACTTGAG CCAGTGGCTT CTGCGCCAGC TCTACCGCGT 
GAACAGCAAT CCCCGGCACA ACCTCCGCCA CCACCACAAC AGCAGCAACC ACCGCAGCAG 
CAGGTACCTC CACCGCCATC CTCTGCGGAC GTGGACGCTC AAAATGCGGC CGCTATGATG 
ATGGCAGCGG GCGGAGCTAA TAGTACTCCT GGATCGGTAT CTTCGCCCAA TCTACCCACC 
GATTCCAGCC AGGTGGCACT ACCGCCACCA GAGCCGCGCT ATTCATACGT GGACGTAAAC 
GTGAGCAACT TCCAGCTTAT TGGTCAGCAG TTGGTACTGC CACCCAATAC TCCGTTCCTG 
CACGCCAATC CCGGAATCAA ACACATGGTC GTCAACGCCG TGGAGCGCAC GATAACCGAC 
TGGTTGCAGC CTATTGTGGA CCGAAGCATC CGCATTGCCT GTGCCACCAC CGAGCAGATC 
ATCCGCAAGG ACTTCGCCCT GGATGCTGAC GAGAACCGAA TGCGCACTGC CGCTCATCAG 
ATGGTACGAA ACCTTGCCGC CGGCATGGCC ATGATTACCG GTAAGGATGA GATAGCGCGT 
GCTATTAGCC AGAATCTGCA CAAGGCCTTG CTGTCGGGTC TAAATGGGAT GCCCAGCATG 
GCTGAGATCC AAGCTGCTGC TATGCAGTTA GCCAGCGAAA ATGTTGAGCT AGTTTGCGCT 
TTCATTCAGA AAACATCTGC TGAAAAGGCA GCCGCAGAGA TCGATAGGCG TCTAAGCACC 
GATTTCGAGA CCAGAAAAAT TGCTCGTGAA GAGGGCAACC GCTTTGTGGA CGCCCAGATC 
CTCACCTATC AACAGGAGCG TCTTCCAGAA GCAGTGCGCA TCAAGGTGGG CGCGGCTCCA 
GCTACACTTT ATGCTGTGTA CTCGGAGTTC GCTAGAAGCA TACCTGGCTT CCAGCAGATG 
AGCGATCGCG ATATTGCCCT GTTTGTGCCC AAGCCGACGG ATTTGTCTCA GCCAAATGTG 
TTTGCTAATG ATGATAGCAT GGTATACGGG GAACTGGCTA GCAAAATGGA GGCCTTCATG 
AACACGGCAA TCGGAGTGCC GACACTCCAG ATCCAGGCTA GCAAGATGCA CATGCTACTC 
AACGCCCTGA TAGCGACGCG TCGACTGCGT GACCAAGAGT CTGCCTTTAA TCTGCTGACC 
CGCGCGGTCG AGGGTTTGAC CGAAGGATTG GTCAATATGC ATGAAAACAT GGAGCAAATG 
AAGATGTACC AAAACATCCA TCTCCGTATC CTTGGCTTGC TGAACAATAG TTTCGGTGCT 
CCAAACACAG AGCGAGCGGT AACAAAGTGT TTCTTCGATA TCCGAGAGGA GGTGCGATAT 
AACGTGGAGG CAGCTCGCGC TTTGATTACG TCGCACTTTG TCAATCTGAA TCAGTTTGAC 
GGAATGCTAC GCGACTGCAT GGACAACGGA AACAATTATG TGGCCATATC GTTCGGTATC 
GCACTGCTGG AGCGCCTCAT CATGGACGAT CGAGTGATTA ATATTGTTTC GGACAACGAA 
TTCATGGCAA CTGTTGAGCT GCTAGGTAGG CTCACTCAGC ATCGCCATCG GTATCCAGAA 
TGCATTGTGA ATGCTATTGA TACGCTCTGG AGCGGAAACT TTAACTCGAG CAGCGACTAC 
AGCCCGTTCA ACGGCAATGA TCGTTACTTG TCGGGAGCCT CCCATTACAT CCACTCCGGC 
ATGCATCACG TAAGGTCATG CGATACAGAT GATCCGCCGG GCTTACAAGA GAAGACGGAG 
TTTCTGCTTA AGGATTGGGT TGCACTGTAC ACGCAACAGA ACCAGCAGTC AACGCGCGAT 
GCACGCAACT TCGGTGCATT TGTTCAGAAG ATGAACACAT ATGGTATCCT GAAAACGGAC 
GACTTGATCA CCCGTTTTTT CCGCCAGGCC ACACACATTT GCACGGATGT GGTGTACAGA 
ATGTTCGCCG AGCCGAGTTT ACCAATCAAC CAGGCCAAAA ACAAGATATT CCAGTGGATC 
GATGCGTTCG TCCATCTGAT TGCAATGCTC GTGCGACACT CAGGCGAAGC AGGTAATCCA 
ACCACCAAGA TCAACCTGTT AAACAAGGTG CTGGGCATTG TGCTGGGAAC GCTGATTAAG 
GACCATGAGA TGCGTGGCGT TAGCTTCCAG CAGGTTGGCT ACCATCGCTT CTTCATGATG 
CTGTTCATGG AGCTGTGCAC GGCCGATGTG ATTCTCGAGT CCCTGATGCA CAGTATTGTG 
TCGGCCTTCG CCTACACATA TCACTTGTTG AATCCCAGCG TGGCTCCGGG ATTCTGCTTT 
GCTTGGTTGG AGCTCATTTC GCATCGCGTC TTCCTGGGCC GCATCCTGGT CCAAATTCCC 
GGGCAAAAGG GCTGGCCACT TTACGCTCAG TTGCTGCAGG ACCTTTTTAA ATATCTTGCG 
CCCTTCTTAC GCAACACTGA GCTCGGCAAA CCAGTCCAGC TTTTGTACAA GGGGACGCTA 
CGTGTTCTGC TCGTTCTGCT TCACGACTTC CCGGAGTTCT TGTGCGACTA TCATTTTGGG 
TTCTGTGACA CCATCCCGCC CAATTGCGTC CAGATGCGTA ACATCATCCT GTCGGCGTTT 
CCGCGCAACA TGCGCTTGCC CGATCCCTTC ACGCCCAACC TAAAAGTGGA CATGCTGTCG 
GACAGTAGTA ATGCGCCTAA GGTGCTCAGT AGCTACATTA TGAACATCCA GCCCGCGAAC 
TTTAAGAAGG ATTTGGACTC GTACCTCAAG GCCCGGGCGC CAGTTACTTT TCTATCGGAA 
TTACGTGGTC ACTTGCAGGT GACAAGCGAG CCAGGAACTC GTTACAACAT GGCACTGATG 
AATGCTCTGG TCATGTATGT GGGAACTCAG GCGATTGCTT TGATCAGAAA CAAAAACTTT 
GTGCCCAACA CCTCGAACAT AGCACACAGC GCCCACATGG ATATCTTCCA GAATCTTGCT 
GTTGACTTGG ATACGGAGGG TCGCTATCTG TTCTTGAATG CGATTGCGAA CCAGTTGCGC 
TATCCCAACA GCCACACGCA CTACTTCAGC TGTGCGGTGC TGCATCTGTT TGCCGAGGCC 
AACTCGGAGG CAATTCAGGA GCAGATCACT CGCGTACTTC TGGAACGATT GATCGTGAAC 
CGCCCGCATC CTTGGGGACT GTTGATCACT TTTATTGAGC TGATCAAAAA TCCCATCTAC 
AAGTTCTGGG ACCATGACTT TGTGCACTGT GCGCCAGAGA TCACCAAGCT CTTTGAATCG 
GTGGCCCGTT CCTGTCTGGC CAAGTCGAAC GTCACCCAGC AGTTGAACAT GCCCGTCGTC 
GATGGCGAGG GCCAGGAGGT AGCCACCATC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286252.1
CDS	192..7727
Translation	MCPRLRAVAVKALILASKPYAMQLFDSKPYITDSIYDILQFVKEHGLEADRSSLRHLFSVINFSDLVPSVTVQLQAKLLGIQLERQLHSSSFVSNICYAFDQFFASNQKQSLKPVAVADLVGQVARLTGINKVCECVFALALTHSSYTELKHSARNNLKGSLSELIDSYLGNKGTGPADSGLREISFDLLQYLLCCLSEYVQPQVEAQFLIKLREEFPRQAVPLVLAPFLYGSTTATIAGAGASETDAEAEATTNSNSSSFEADALNEVGIEDIYDHLSEIIFTNQGKNNIMDTSWINLILEIGYEFTSSVEECKNHLCSRERERAELQSKDVAKIVGLMCRRHSSLLDCNVNLPTPANFWPGQGQGGGSNSSGSSQTQITPQQQNPGSSNNNDGSDGNSSSDKKDKKETTEATQTWKPDVFVQALKEVVPQLNWKDVCMELDHPEFVLKDRIGLELLLTILRLATGSNIFPHPECIYRHWANTEGQLSLIATMLKNPDLFSFADFVFSQPALDVLKTAPDADNKEISSWKSLHLVEVLLSIADKGYYTQVHELFKFPAQNCPDVLFLALLNTSPPMTPLRQDLFNQLIPTFLGNHPNSNVILASAWSSNNFQLRSNIMNAMSEWYLRGNEFDQVKLSRILDLAQDLKALSALLNARSFLFIIDLACLASRREYLKLEKWLTDKIREHGEPFMQAIIKVLHRRCPQVINAKVPEDQLPPKQAQLLPETVTTMINCLQTCINNCMQPEMVEVIMQMTANVAIMANKARAQQQQQPGLVPPPPPTILRGHRGMDLPGGIVPPPPQQPFSGNLNAQMFGPGMDPLTNMSNNLAGLNLSGPNGAFNFGNMLTSPSRLMTPGANPYPLNLMQMPQAPPPPNVGNLGRMLPGGPQQQTPTPTPTAPNPNNPVMADLQIPVSKEVEDEVNSYFQRIYNHQPNPTLSIDEVLDILQRFKESSNRREQEVFLCMLRNLFEEYRFFCQYPEKELQITAQLFGGIIDRNLVPTFVALGLSLRCVLDALRKPDGSKLYYFGVTALDRFRTRLHTYNKYCEHIRSIPHFSDFPPHLIQYVEYGMHGQEPPPQKLIGLSNTIPSAISSGPGTEPIYRNSSMLGNMPAATPGSGPKSNAAVSHATRMKSIANATNIDTLLVANQEEKVTVPPEPVQDKTAFIFNNLSQLNIPQKCDEIKEIMTKEYWPWLAQYLVLKRASMEFNFHTLYYNFLDALKNGEINRFVTKETLRNIKVLLRSDKGVINFSDRSLLKNLGHWLGMMTLGRNRPILQLDLDLKSLLAEAYHKGQQELLFVVPFVAKILESSAKSRIFRSPNPWTMGIMYVLAELHQEPDLKLNLKFEIEVLCKTLNLELAKLRQVIYLKDPNRTHRIEEQMSQPKPKQLEPVASAPALPREQQSPAQPPPPPQQQQPPQQQVPPPPSSADVDAQNAAAMMMAAGGANSTPGSVSSPNLPTDSSQVALPPPEPRYSYVDVNVSNFQLIGQQLVLPPNTPFLHANPGIKHMVVNAVERTITDWLQPIVDRSIRIACATTEQIIRKDFALDADENRMRTAAHQMVRNLAAGMAMITGKDEIARAISQNLHKALLSGLNGMPSMAEIQAAAMQLASENVELVCAFIQKTSAEKAAAEIDRRLSTDFETRKIAREEGNRFVDAQILTYQQERLPEAVRIKVGAAPATLYAVYSEFARSIPGFQQMSDRDIALFVPKPTDLSQPNVFANDDSMVYGELASKMEAFMNTAIGVPTLQIQASKMHMLLNALIATRRLRDQESAFNLLTRAVEGLTEGLVNMHENMEQMKMYQNIHLRILGLLNNSFGAPNTERAVTKCFFDIREEVRYNVEAARALITSHFVNLNQFDGMLRDCMDNGNNYVAISFGIALLERLIMDDRVINIVSDNEFMATVELLGRLTQHRHRYPECIVNAIDTLWSGNFNSSSDYSPFNGNDRYLSGASHYIHSGMHHVRSCDTDDPPGLQEKTEFLLKDWVALYTQQNQQSTRDARNFGAFVQKMNTYGILKTDDLITRFFRQATHICTDVVYRMFAEPSLPINQAKNKIFQWIDAFVHLIAMLVRHSGEAGNPTTKINLLNKVLGIVLGTLIKDHEMRGVSFQQVGYHRFFMMLFMELCTADVILESLMHSIVSAFAYTYHLLNPSVAPGFCFAWLELISHRVFLGRILVQIPGQKGWPLYAQLLQDLFKYLAPFLRNTELGKPVQLLYKGTLRVLLVLLHDFPEFLCDYHFGFCDTIPPNCVQMRNIILSAFPRNMRLPDPFTPNLKVDMLSDSSNAPKVLSSYIMNIQPANFKKDLDSYLKARAPVTFLSELRGHLQVTSEPGTRYNMALMNALVMYVGTQAIALIRNKNFVPNTSNIAHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCAVLHLFAEANSEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPIYKFWDHDFVHCAPEITKLFESVARSCLAKSNVTQQLNMPVVDGEGQEVATIN

Target ORF information:

RefSeq Version	NM_001299323.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Not1, transcript variant H (Not1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299323.1

ORF Insert Sequence:

ATGTGTCCCA GATTGCGGGC AGTCGCTGTC AAGGCACTTA TATTGGCTTC CAAACCCTAC 
GCAATGCAGC TCTTTGATTC TAAACCATAC ATTACTGATA GCATTTACGA CATCTTGCAG 
TTCGTTAAAG AGCACGGCCT GGAAGCGGAT CGCAGCAGCC TGCGCCACCT GTTCTCCGTG 
ATCAACTTCA GCGATCTCGT GCCATCAGTT ACGGTCCAGC TGCAGGCGAA GCTCCTGGGC 
ATCCAGTTGG AGCGTCAGCT TCACAGTTCA TCGTTTGTGT CCAACATCTG CTACGCTTTT 
GACCAGTTCT TCGCCAGCAA CCAGAAGCAG TCCCTGAAGC CTGTTGCGGT AGCGGATCTC 
GTTGGCCAGG TCGCCCGACT GACCGGCATC AACAAGGTTT GTGAGTGCGT CTTTGCCTTG 
GCCTTGACCC ACTCCTCGTA CACAGAACTC AAACATTCTG CCAGGAACAA CCTAAAAGGT 
AGCCTCAGCG AACTTATAGA CTCTTACTTG GGTAATAAAG GCACCGGCCC AGCCGACAGC 
GGTCTCCGGG AGATCTCCTT CGATCTACTG CAGTATTTGT TGTGCTGCCT TAGCGAGTAC 
GTACAACCGC AGGTGGAAGC ACAGTTTTTG ATCAAGCTTC GTGAGGAGTT TCCGCGCCAG 
GCGGTACCGC TGGTTCTAGC GCCGTTCCTT TACGGCTCGA CGACGGCGAC GATTGCGGGA 
GCAGGTGCCA GCGAAACGGA TGCGGAGGCC GAAGCGACGA CTAATAGCAA CAGCTCCAGT 
TTCGAAGCAG ATGCTTTGAA CGAGGTGGGA ATCGAGGATA TTTATGACCA TTTAAGCGAG 
ATAATATTCA CCAACCAGGG CAAAAATAAT ATTATGGACA CATCTTGGAT TAATCTAATC 
CTTGAAATCG GTTATGAATT TACATCGAGC GTTGAAGAGT GTAAGAATCA TTTGTGTTCC 
CGCGAGCGAG AACGCGCTGA GCTCCAGTCC AAAGATGTCG CCAAGATCGT GGGTCTTATG 
TGCCGACGGC ACTCCTCCCT GCTCGATTGT AATGTGAATC TACCGACGCC GGCGAACTTC 
TGGCCTGGTC AGGGACAGGG CGGTGGCAGC AACAGCAGTG GCTCTTCGCA GACACAAATC 
ACTCCGCAGC AACAGAACCC GGGCAGCAGT AACAACAACG ATGGCAGCGA TGGCAATTCG 
TCCAGTGATA AGAAGGACAA GAAGGAGACG ACGGAGGCCA CGCAAACTTG GAAGCCAGAT 
GTCTTTGTGC AGGCGCTCAA GGAGGTGGTG CCTCAGCTCA ACTGGAAGGA CGTGTGCATG 
GAACTTGATC ATCCCGAGTT CGTGCTGAAG GATCGCATCG GTTTGGAGCT TTTGCTCACC 
ATCCTTCGGC TGGCCACTGG CTCTAACATA TTTCCACATC CAGAATGCAT TTACCGTCAC 
TGGGCAAACA CGGAAGGTCA GCTGTCGCTT ATCGCGACAA TGCTGAAGAA CCCGGATCTC 
TTTTCCTTCG CCGACTTTGT ATTTAGCCAG CCAGCGTTGG ATGTGCTTAA GACGGCTCCG 
GATGCGGACA ATAAGGAGAT CTCGTCCTGG AAGTCGCTTC ACCTGGTGGA GGTGCTGCTC 
TCCATTGCGG ACAAGGGATA CTATACGCAG GTCCATGAGC TATTCAAGTT CCCAGCGCAG 
AACTGTCCCG ATGTGCTATT TTTGGCCTTA TTGAACACAA GTCCGCCTAT GACGCCACTG 
CGTCAGGATC TGTTTAACCA ACTGATACCA ACGTTCCTTG GCAACCATCC CAACTCGAAC 
GTGATCTTGG CCAGCGCCTG GAGCTCGAAC AACTTCCAAC TTCGTTCCAA TATCATGAAT 
GCTATGTCCG AGTGGTATCT CCGAGGCAAT GAGTTCGATC AGGTGAAACT GTCGCGCATC 
CTGGATCTTG CCCAGGATTT AAAAGCGCTA TCAGCGCTGC TCAACGCCCG TTCATTTTTA 
TTCATTATCG ACCTGGCTTG CCTTGCCTCT CGGCGCGAGT ACCTGAAGCT GGAAAAGTGG 
CTTACCGATA AAATTCGCGA ACATGGAGAG CCTTTTATGC AGGCTATAAT CAAGGTTCTG 
CACCGCCGCT GCCCGCAGGT TATCAACGCA AAAGTGCCAG AGGATCAGCT GCCTCCCAAG 
CAGGCGCAGC TTTTGCCCGA GACAGTTACC ACCATGATCA ACTGTCTGCA AACGTGCATC 
AATAACTGCA TGCAGCCGGA GATGGTCGAA GTCATCATGC AAATGACCGC GAACGTGGCG 
ATTATGGCTA ACAAGGCGCG TGCCCAACAG CAGCAGCAGC CAGGATTGGT TCCGCCGCCT 
CCACCAACAA TTCTGCGCGG CCATCGTGGT ATGGATCTGC CTGGAGGCAT AGTCCCTCCG 
CCACCGCAGC AACCGTTCTC CGGAAACCTC AACGCGCAGA TGTTTGGACC AGGAATGGAT 
CCGCTAACGA ATATGTCCAA CAACTTAGCC GGCCTCAACC TCAGCGGCCC GAACGGAGCA 
TTTAATTTTG GAAACATGCT AACTTCTCCA TCGCGTCTGA TGACTCCGGG AGCCAATCCC 
TATCCGCTGA ACCTCATGCA GATGCCGCAG GCTCCGCCAC CACCCAACGT AGGAAACCTA 
GGCCGCATGC TGCCAGGGGG TCCACAACAG CAGACTCCAA CGCCGACTCC AACGGCCCCC 
AATCCAAACA ACCCTGTGAT GGCCGATCTC CAGATACCTG TGTCAAAGGA AGTTGAAGAC 
GAGGTCAACT CATACTTTCA GCGCATCTAC AACCACCAGC CGAATCCAAC GCTGTCCATC 
GATGAAGTGC TGGACATTTT GCAAAGATTT AAAGAGTCAA GTAACCGGCG CGAGCAGGAA 
GTCTTTTTGT GCATGCTGCG CAATCTGTTC GAGGAATATC GCTTTTTCTG TCAATACCCA 
GAGAAAGAGC TACAGATTAC TGCGCAGCTA TTTGGTGGCA TTATTGATCG TAACCTGGTG 
CCCACTTTTG TTGCACTGGG CCTTTCTCTG CGCTGTGTCT TGGATGCCCT CCGCAAGCCC 
GATGGATCTA AGCTTTACTA TTTCGGTGTG ACGGCACTGG ACAGATTCAG AACTCGATTG 
CACACCTACA ACAAATACTG CGAGCATATC CGCTCCATTC CTCACTTCTC TGACTTCCCG 
CCGCACCTTA TTCAGTATGT GGAATACGGA ATGCACGGCC AGGAGCCGCC GCCGCAGAAG 
TTGATTGGAC TCAGCAATAC GATTCCTTCG GCGATATCTA GCGGACCAGG AACGGAACCA 
ATTTACAGGA ATAGCTCCAT GTTAGGTAAT ATGCCCGCTG CCACCCCGGG ATCGGGACCG 
AAATCAAACG CTGCTGTATC GCATGCCACG AGAATGAAGT CCATCGCCAA TGCCACCAAT 
ATCGACACAC TTTTGGTGGC CAACCAGGAA GAGAAAGTGA CTGTGCCACC AGAGCCTGTG 
CAGGACAAAA CTGCCTTTAT TTTCAACAAC TTGAGCCAGC TGAACATACC GCAGAAGTGC 
GATGAGATAA AGGAGATTAT GACCAAGGAG TATTGGCCGT GGCTAGCGCA ATATCTGGTC 
CTCAAACGCG CATCAATGGA GTTCAACTTC CACACACTAT ATTACAACTT TTTGGATGCC 
CTTAAAAACG GCGAGATCAA CCGATTCGTA ACCAAAGAGA CCCTACGCAA CATCAAGGTA 
CTCTTGCGCT CCGATAAGGG AGTTATTAAT TTCTCCGATC GAAGTCTGTT GAAGAACCTC 
GGACACTGGT TGGGCATGAT GACCTTAGGT CGCAATCGCC CCATCCTGCA GTTGGATCTG 
GATCTTAAAT CCCTTTTGGC TGAGGCTTAC CACAAGGGCC AGCAGGAACT GCTTTTTGTG 
GTTCCTTTCG TAGCCAAGAT CCTTGAGTCA TCCGCCAAGT CTCGCATCTT CCGATCGCCC 
AATCCATGGA CAATGGGTAT CATGTACGTG CTTGCTGAGC TTCACCAGGA GCCAGACCTT 
AAGCTGAACC TGAAGTTTGA AATCGAAGTA CTCTGTAAAA CACTTAATCT GGAGTTGGCC 
AAGTTGCGAC AAGTGATCTA CCTGAAGGAT CCCAATCGAA CTCACCGGAT TGAAGAGCAG 
ATGTCGCAAC CAAAACCGAA ACAACTTGAG CCAGTGGCTT CTGCGCCAGC TCTACCGCGT 
GAACAGCAAT CCCCGGCACA ACCTCCGCCA CCACCACAAC AGCAGCAACC ACCGCAGCAG 
CAGGTACCTC CACCGCCATC CTCTGCGGAC GTGGACGCTC AAAATGCGGC CGCTATGATG 
ATGGCAGCGG GCGGAGCTAA TAGTACTCCT GGATCGGTAT CTTCGCCCAA TCTACCCACC 
GATTCCAGCC AGGTGGCACT ACCGCCACCA GAGCCGCGCT ATTCATACGT GGACGTAAAC 
GTGAGCAACT TCCAGCTTAT TGGTCAGCAG TTGGTACTGC CACCCAATAC TCCGTTCCTG 
CACGCCAATC CCGGAATCAA ACACATGGTC GTCAACGCCG TGGAGCGCAC GATAACCGAC 
TGGTTGCAGC CTATTGTGGA CCGAAGCATC CGCATTGCCT GTGCCACCAC CGAGCAGATC 
ATCCGCAAGG ACTTCGCCCT GGATGCTGAC GAGAACCGAA TGCGCACTGC CGCTCATCAG 
ATGGTACGAA ACCTTGCCGC CGGCATGGCC ATGATTACCG GTAAGGATGA GATAGCGCGT 
GCTATTAGCC AGAATCTGCA CAAGGCCTTG CTGTCGGGTC TAAATGGGAT GCCCAGCATG 
GCTGAGATCC AAGCTGCTGC TATGCAGTTA GCCAGCGAAA ATGTTGAGCT AGTTTGCGCT 
TTCATTCAGA AAACATCTGC TGAAAAGGCA GCCGCAGAGA TCGATAGGCG TCTAAGCACC 
GATTTCGAGA CCAGAAAAAT TGCTCGTGAA GAGGGCAACC GCTTTGTGGA CGCCCAGATC 
CTCACCTATC AACAGGAGCG TCTTCCAGAA GCAGTGCGCA TCAAGGTGGG CGCGGCTCCA 
GCTACACTTT ATGCTGTGTA CTCGGAGTTC GCTAGAAGCA TACCTGGCTT CCAGCAGATG 
AGCGATCGCG ATATTGCCCT GTTTGTGCCC AAGCCGACGG ATTTGTCTCA GCCAAATGTG 
TTTGCTAATG ATGATAGCAT GGTATACGGG GAACTGGCTA GCAAAATGGA GGCCTTCATG 
AACACGGCAA TCGGAGTGCC GACACTCCAG ATCCAGGCTA GCAAGATGCA CATGCTACTC 
AACGCCCTGA TAGCGACGCG TCGACTGCGT GACCAAGAGT CTGCCTTTAA TCTGCTGACC 
CGCGCGGTCG AGGGTTTGAC CGAAGGATTG GTCAATATGC ATGAAAACAT GGAGCAAATG 
AAGATGTACC AAAACATCCA TCTCCGTATC CTTGGCTTGC TGAACAATAG TTTCGGTGCT 
CCAAACACAG AGCGAGCGGT AACAAAGTGT TTCTTCGATA TCCGAGAGGA GGTGCGATAT 
AACGTGGAGG CAGCTCGCGC TTTGATTACG TCGCACTTTG TCAATCTGAA TCAGTTTGAC 
GGAATGCTAC GCGACTGCAT GGACAACGGA AACAATTATG TGGCCATATC GTTCGGTATC 
GCACTGCTGG AGCGCCTCAT CATGGACGAT CGAGTGATTA ATATTGTTTC GGACAACGAA 
TTCATGGCAA CTGTTGAGCT GCTAGGTAGG CTCACTCAGC ATCGCCATCG GTATCCAGAA 
TGCATTGTGA ATGCTATTGA TACGCTCTGG AGCGGAAACT TTAACTCGAG CAGCGACTAC 
AGCCCGTTCA ACGGCAATGA TCGTTACTTG TCGGGAGCCT CCCATTACAT CCACTCCGGC 
ATGCATCACG TAAGGTCATG CGATACAGAT GATCCGCCGG GCTTACAAGA GAAGACGGAG 
TTTCTGCTTA AGGATTGGGT TGCACTGTAC ACGCAACAGA ACCAGCAGTC AACGCGCGAT 
GCACGCAACT TCGGTGCATT TGTTCAGAAG ATGAACACAT ATGGTATCCT GAAAACGGAC 
GACTTGATCA CCCGTTTTTT CCGCCAGGCC ACACACATTT GCACGGATGT GGTGTACAGA 
ATGTTCGCCG AGCCGAGTTT ACCAATCAAC CAGGCCAAAA ACAAGATATT CCAGTGGATC 
GATGCGTTCG TCCATCTGAT TGCAATGCTC GTGCGACACT CAGGCGAAGC AGGTAATCCA 
ACCACCAAGA TCAACCTGTT AAACAAGGTG CTGGGCATTG TGCTGGGAAC GCTGATTAAG 
GACCATGAGA TGCGTGGCGT TAGCTTCCAG CAGGTTGGCT ACCATCGCTT CTTCATGATG 
CTGTTCATGG AGCTGTGCAC GGCCGATGTG ATTCTCGAGT CCCTGATGCA CAGTATTGTG 
TCGGCCTTCG CCTACACATA TCACTTGTTG AATCCCAGCG TGGCTCCGGG ATTCTGCTTT 
GCTTGGTTGG AGCTCATTTC GCATCGCGTC TTCCTGGGCC GCATCCTGGT CCAAATTCCC 
GGGCAAAAGG GCTGGCCACT TTACGCTCAG TTGCTGCAGG ACCTTTTTAA ATATCTTGCG 
CCCTTCTTAC GCAACACTGA GCTCGGCAAA CCAGTCCAGC TTTTGTACAA GGGGACGCTA 
CGTGTTCTGC TCGTTCTGCT TCACGACTTC CCGGAGTTCT TGTGCGACTA TCATTTTGGG 
TTCTGTGACA CCATCCCGCC CAATTGCGTC CAGATGCGTA ACATCATCCT GTCGGCGTTT 
CCGCGCAACA TGCGCTTGCC CGATCCCTTC ACGCCCAACC TAAAAGTGGA CATGCTGTCG 
GACAGTAGTA ATGCGCCTAA GGTGCTCAGT AGCTACATTA TGAACATCCA GCCCGCGAAC 
TTTAAGAAGG ATTTGGACTC GTACCTCAAG GCCCGGGCGC CAGTTACTTT TCTATCGGAA 
TTACGTGGTC ACTTGCAGGT GACAAGCGAG CCAGGAACTC GTTACAACAT GGCACTGATG 
AATGCTCTGG TCATGTATGT GGGAACTCAG GCGATTGCTT TGATCAGAAA CAAAAACTTT 
GTGCCCAACA CCTCGAACAT AGCACACAGC GCCCACATGG ATATCTTCCA GAATCTTGCT 
GTTGACTTGG ATACGGAGGG TCGCTATCTG TTCTTGAATG CGATTGCGAA CCAGTTGCGC 
TATCCCAACA GCCACACGCA CTACTTCAGC TGTGCGGTGC TGCATCTGTT TGCCGAGGCC 
AACTCGGAGG CAATTCAGGA GCAGATCACT CGCGTACTTC TGGAACGATT GATCGTGAAC 
CGCCCGCATC CTTGGGGACT GTTGATCACT TTTATTGAGC TGATCAAAAA TCCCATCTAC 
AAGTTCTGGG ACCATGACTT TGTGCACTGT GCGCCAGAGA TCACCAAGCT CTTTGAATCG 
GTGGCCCGTT CCTGTCTGGC CAAGTCGAAC GTCACCCAGC AGTTGAACAT GCCCGTCGTC 
GATGGCGAGG GCCAGGAGGT AGCCACCATC AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Not1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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