RhoGAP1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAP1A
Entrez Gene ID	5740235
Full Name	Rho GTPase activating protein at 1A
Synonyms	BCR,CG17617,CG17960,CG40453,CG40494,Dmel\CG40494,Dmel_CG00000,EG:23E12.2,EG:23E12.5,RhoGAP,rhoGAP1A
General protein information	Preferred Names Rho GTPase activating protein at 1A Names CG40494 gene product from transcript CG40494-RE CG40494-PA CG40494-PB CG40494-PC CG40494-PD CG40494-PE CG40494-PF RhoGAP1A-PA RhoGAP1A-PB RhoGAP1A-PC RhoGAP1A-PD RhoGAP1A-PE RhoGAP1A-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1A1-1A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297797.1	NP_001284726.1	Rho GTPase activating protein at 1A, isoform F
NM_001297795.1	NP_001284724.1	Rho GTPase activating protein at 1A, isoform D
NM_001103385.2	NP_001096855.1	Rho GTPase activating protein at 1A, isoform A
NM_001103386.2	NP_001096856.1	Rho GTPase activating protein at 1A, isoform B
NM_001297796.1	NP_001284725.1	Rho GTPase activating protein at 1A, isoform E
NM_001169155.1	NP_001162626.1	Rho GTPase activating protein at 1A, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABR	NP_068781.2
Bos taurus (cattle)	ABR	NP_001039669.2
Mus musculus (house mouse)	Abr	NP_942598.1
Rattus norvegicus (Norway rat)	Abr	NP_001099284.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000865	XP_316836.5
Gallus gallus (chicken)	LOC771897	XP_004946703.1
Canis lupus familiaris (dog)	ABR	XP_537757.4
Drosophila melanogaster (fruit fly)	RhoGAP1A	NP_001096855.1
Xenopus tropicalis (tropical clawed frog)	abr	NP_001072313.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RhoGAP1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAP1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03009	NM_001297797.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03009	NM_001169155.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa02623	NM_001103385.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa02623	NM_001103386.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22406	NM_001297796.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa22407	NM_001297795.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAP1A ( NM_001297797.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284726.1 Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa03009
Clone ID Related Accession (Same CDS sequence)	NM_001297797.1 , NM_001169155.1
Accession Version	NM_001297797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 1A, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACCCA GACCTCCGCC AAAGAATATT CGGCGAGTTG AGCCCCCTAC CATTCCCAGC 
CAGCAGCTAT CGTCTTCGCC CAAAAGGGAG ACATATATGA TCAACACGAT GCCAAGCAGT 
TCAAGCGGCA GTCTCTGCAT TGACAGCCTG GGCAATTCGC TGGAGCGGAA CATCAAGCCG 
TCTGATATAC TACGTCAGAA ATCATCTGAT AATTTGGATT CCAAGTACTC GGCAAAACGG 
AAAACCACAC CGGAGCTTAG TAAATTTGTG AACGTAAACA ATCCTGAATT AATGGAGGAG 
CTCGGTCGTA AGATGGTAAG CAAGGCAGAC AGCCTGGAAC AAACGAGGAG GAACGACTCG 
TCGCCTTCTG GCTGTGGTAG CCTAGGACGC ACTTCCTCCA CGCCTGGGCG ATCGTTGACT 
CCTGGACGAA CCGGAGTTGG TGCCTCGCCA ACTGGTAGTC TAAGTAAAAC CGGAAAACTG 
GCAATGGCGG ACAGCAGCTC CACTAGCAGT CTAGAGAGGA GATCGTGCCT GCAGGCTGTC 
GATGCGGACA TTGGAACGGA ATCGCGGGAA TCGGGTGCCA ACCAGCGTAG TGTAGGGTCC 
AAGGAGTCGC TGACATCACA AGGCATATCG GAGGTGATCA AGAGCTACGA AAGCGTGTCT 
TCGCTAAGTT CGGAAAGTGC AAAGGCGGTG GCTTCCCAAC AGTCGGCGGC AGCGGGCGGA 
GCTTTAGCGG AGTGTGAACC GTACTATGAT AGCGTACCGC TCGACAACGG CGACGGAGAA 
TACGTTTTTA TTAAGCCCGG CCGCACCGGA TCCTCATCCA GTCGCGATGA TTTGTCGACG 
CCTGGGAGCA GCATTTTGCC TTTGCCTAAT GTAGCGGATC CCGAATCTCC AGGCAGGACC 
TCTAACTACG TCAACATCGA TTTCTTCCTT CAGCAAGGAA TTGAGACGCG CTCCAGTTCG 
CTAGACAGTG ACGGGGAGTA CGAGGGTCCG CCCATATTAC GCACCATTTC GCACGACGAT 
CAAACCACAA CACAAACTAC TCCGGGAGCA ATTCGCAAGG TTTGTGGAAA TGCGCGCGGT 
GCTAAAATAC GTTCCATACT TAGCTCAATT ATTCAAAGTG AGACTATCTA CGTGGAATGC 
CTCAATAAAA TGATGCAGTA CAAGAAGGCC ATCCACGCAA CCCTAACTAC TTCGCAGCCT 
GTGATCAGGG AGGAGGAGGA GAACACAATT TTCTTTAAAA TTGATGAGCT TTACGATCTG 
CATACCGGTT TCTTATCTGA TTTAAAGACT ATTGTGTCTC ACGAGGGTGG TGATGTGCTG 
ATCGGTGAGC CGTTTCGTCG ACTGGCGGAG ATGTTTGACT TATACAGTGC CTTTTTGCAC 
AACTACAAAA ATGCTATTGA GACTGTGAAG AAGTGCAGTG CCAATAACCC CCAGTTTAAA 
AAGATCGTCT CCACCATTGT GCTTAATCTG CAGACGGAAC AATCGCTAAC TCTCGAGGAT 
CTATTGCACA AACCTGTTGC GAGGGTACAA ATCAACGCAT TGGTCTTCAA CGATTTGCTA 
CGCGAAACCC CAAATGCGCA TCCGGATCAC CAGCCATTAA GGCAGGCGCA AAAGATTATT 
CAGATGTTCC TCAATCAGTT CAATGTGGTC AATCAGCGGT TGCCCATTGA ATCGAATCGA 
AATTTAAGAC GCATGGTGCG TAATTCTTTT ATTGTAGAAC TTGTCGATGG ACATCGTAAG 
CTGCGGCATC TCTTTCTTTT TAATGATGTC ATCGCATGTG CCAAATACAA AGCATTAGGC 
CGCGATCGTA TCGACTATGA GCTCAAATGG TTCATTCCGC TAAAAGACGT ATCCATTTAC 
GAGGAAGCAG ATCCGGCGGT CGAGCTTAAG GAATCTAGTC CAGCAAACAT TTCGCAAGTA 
AAACGCAATT TGCGATCCGT TCGAGATCAA TTGGCCATGG AGGTGGCTAA CAGTGGAAGT 
GGAGCCTTCC GTTCCGGTGA TAAGTATCGC CGCAAGCTGG CGGATCTGGA ATCACAGCTG 
GTGCTGGCAA CGCCCAATTT AGTGTTGCGC CTGGGGAACA AGGCAAATAA TAAAACCATA 
ACCTTCTTTC TTAGCTCGGA TTTTGAGAGG ACGCAGTGGA TCGACTCAAT CTTATCCCTT 
AAGCAAAAAT GCAACCTGCC TGGAGCAAAT ACTATTAATT CCCTGGAGGT TACCGCCTTC 
ATTGTAGCCA TGCAGAAGGG CATGAAAACC GAAATGGGTT CATATCTTAT GCGTAACACT 
AACGACGAGA GCCTGTTGGT TGGGGACCTG TACATGGGAG TTCATGGATT GGAAGGACTG 
GAGCAAGCGA ACGACCTGTA CATCTGCGTC GAGGTGGACT CCTATGGCCA CTATTTTCGC 
AAGGCCACCA CCAAGAAGAT ATGTCGCAGT CAAACGCCGC TCTGGAACGA AAGCTTTATG 
CTGGAACTGG AGGGCAGCCA GAATGTACGC ATTCTTCTTT ATGAGGCTAA GGAGCGACCG 
CTTCTTAAAG CCAAGCACAT ACTTAAGTTG AGTTTAAGTT GGCTGACAGA AACAACGCAG 
CCGAAGTCAA TTAAACTTAC TGAGACCTTA GAGCTCGGCT GTAGCTTCCG TTTTATACCA 
GGCGAGCTCT TCCGCGGCAG CACAAAGCCA GGAGCCCTCT TCGGTGCCAA AATGAGTCAA 
GTATTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284726.1
CDS	1001..3712
Translation	MPPRPPPKNIRRVEPPTIPSQQLSSSPKRETYMINTMPSSSSGSLCIDSLGNSLERNIKPSDILRQKSSDNLDSKYSAKRKTTPELSKFVNVNNPELMEELGRKMVSKADSLEQTRRNDSSPSGCGSLGRTSSTPGRSLTPGRTGVGASPTGSLSKTGKLAMADSSSTSSLERRSCLQAVDADIGTESRESGANQRSVGSKESLTSQGISEVIKSYESVSSLSSESAKAVASQQSAAAGGALAECEPYYDSVPLDNGDGEYVFIKPGRTGSSSSRDDLSTPGSSILPLPNVADPESPGRTSNYVNIDFFLQQGIETRSSSLDSDGEYEGPPILRTISHDDQTTTQTTPGAIRKVCGNARGAKIRSILSSIIQSETIYVECLNKMMQYKKAIHATLTTSQPVIREEEENTIFFKIDELYDLHTGFLSDLKTIVSHEGGDVLIGEPFRRLAEMFDLYSAFLHNYKNAIETVKKCSANNPQFKKIVSTIVLNLQTEQSLTLEDLLHKPVARVQINALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQFNVVNQRLPIESNRNLRRMVRNSFIVELVDGHRKLRHLFLFNDVIACAKYKALGRDRIDYELKWFIPLKDVSIYEEADPAVELKESSPANISQVKRNLRSVRDQLAMEVANSGSGAFRSGDKYRRKLADLESQLVLATPNLVLRLGNKANNKTITFFLSSDFERTQWIDSILSLKQKCNLPGANTINSLEVTAFIVAMQKGMKTEMGSYLMRNTNDESLLVGDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPLWNESFMLELEGSQNVRILLYEAKERPLLKAKHILKLSLSWLTETTQPKSIKLTETLELGCSFRFIPGELFRGSTKPGALFGAKMSQVLK

Target ORF information:

RefSeq Version	NM_001297797.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297797.1

ORF Insert Sequence:

ATGCCACCCA GACCTCCGCC AAAGAATATT CGGCGAGTTG AGCCCCCTAC CATTCCCAGC 
CAGCAGCTAT CGTCTTCGCC CAAAAGGGAG ACATATATGA TCAACACGAT GCCAAGCAGT 
TCAAGCGGCA GTCTCTGCAT TGACAGCCTG GGCAATTCGC TGGAGCGGAA CATCAAGCCG 
TCTGATATAC TACGTCAGAA ATCATCTGAT AATTTGGATT CCAAGTACTC GGCAAAACGG 
AAAACCACAC CGGAGCTTAG TAAATTTGTG AACGTAAACA ATCCTGAATT AATGGAGGAG 
CTCGGTCGTA AGATGGTAAG CAAGGCAGAC AGCCTGGAAC AAACGAGGAG GAACGACTCG 
TCGCCTTCTG GCTGTGGTAG CCTAGGACGC ACTTCCTCCA CGCCTGGGCG ATCGTTGACT 
CCTGGACGAA CCGGAGTTGG TGCCTCGCCA ACTGGTAGTC TAAGTAAAAC CGGAAAACTG 
GCAATGGCGG ACAGCAGCTC CACTAGCAGT CTAGAGAGGA GATCGTGCCT GCAGGCTGTC 
GATGCGGACA TTGGAACGGA ATCGCGGGAA TCGGGTGCCA ACCAGCGTAG TGTAGGGTCC 
AAGGAGTCGC TGACATCACA AGGCATATCG GAGGTGATCA AGAGCTACGA AAGCGTGTCT 
TCGCTAAGTT CGGAAAGTGC AAAGGCGGTG GCTTCCCAAC AGTCGGCGGC AGCGGGCGGA 
GCTTTAGCGG AGTGTGAACC GTACTATGAT AGCGTACCGC TCGACAACGG CGACGGAGAA 
TACGTTTTTA TTAAGCCCGG CCGCACCGGA TCCTCATCCA GTCGCGATGA TTTGTCGACG 
CCTGGGAGCA GCATTTTGCC TTTGCCTAAT GTAGCGGATC CCGAATCTCC AGGCAGGACC 
TCTAACTACG TCAACATCGA TTTCTTCCTT CAGCAAGGAA TTGAGACGCG CTCCAGTTCG 
CTAGACAGTG ACGGGGAGTA CGAGGGTCCG CCCATATTAC GCACCATTTC GCACGACGAT 
CAAACCACAA CACAAACTAC TCCGGGAGCA ATTCGCAAGG TTTGTGGAAA TGCGCGCGGT 
GCTAAAATAC GTTCCATACT TAGCTCAATT ATTCAAAGTG AGACTATCTA CGTGGAATGC 
CTCAATAAAA TGATGCAGTA CAAGAAGGCC ATCCACGCAA CCCTAACTAC TTCGCAGCCT 
GTGATCAGGG AGGAGGAGGA GAACACAATT TTCTTTAAAA TTGATGAGCT TTACGATCTG 
CATACCGGTT TCTTATCTGA TTTAAAGACT ATTGTGTCTC ACGAGGGTGG TGATGTGCTG 
ATCGGTGAGC CGTTTCGTCG ACTGGCGGAG ATGTTTGACT TATACAGTGC CTTTTTGCAC 
AACTACAAAA ATGCTATTGA GACTGTGAAG AAGTGCAGTG CCAATAACCC CCAGTTTAAA 
AAGATCGTCT CCACCATTGT GCTTAATCTG CAGACGGAAC AATCGCTAAC TCTCGAGGAT 
CTATTGCACA AACCTGTTGC GAGGGTACAA ATCAACGCAT TGGTCTTCAA CGATTTGCTA 
CGCGAAACCC CAAATGCGCA TCCGGATCAC CAGCCATTAA GGCAGGCGCA AAAGATTATT 
CAGATGTTCC TCAATCAGTT CAATGTGGTC AATCAGCGGT TGCCCATTGA ATCGAATCGA 
AATTTAAGAC GCATGGTGCG TAATTCTTTT ATTGTAGAAC TTGTCGATGG ACATCGTAAG 
CTGCGGCATC TCTTTCTTTT TAATGATGTC ATCGCATGTG CCAAATACAA AGCATTAGGC 
CGCGATCGTA TCGACTATGA GCTCAAATGG TTCATTCCGC TAAAAGACGT ATCCATTTAC 
GAGGAAGCAG ATCCGGCGGT CGAGCTTAAG GAATCTAGTC CAGCAAACAT TTCGCAAGTA 
AAACGCAATT TGCGATCCGT TCGAGATCAA TTGGCCATGG AGGTGGCTAA CAGTGGAAGT 
GGAGCCTTCC GTTCCGGTGA TAAGTATCGC CGCAAGCTGG CGGATCTGGA ATCACAGCTG 
GTGCTGGCAA CGCCCAATTT AGTGTTGCGC CTGGGGAACA AGGCAAATAA TAAAACCATA 
ACCTTCTTTC TTAGCTCGGA TTTTGAGAGG ACGCAGTGGA TCGACTCAAT CTTATCCCTT 
AAGCAAAAAT GCAACCTGCC TGGAGCAAAT ACTATTAATT CCCTGGAGGT TACCGCCTTC 
ATTGTAGCCA TGCAGAAGGG CATGAAAACC GAAATGGGTT CATATCTTAT GCGTAACACT 
AACGACGAGA GCCTGTTGGT TGGGGACCTG TACATGGGAG TTCATGGATT GGAAGGACTG 
GAGCAAGCGA ACGACCTGTA CATCTGCGTC GAGGTGGACT CCTATGGCCA CTATTTTCGC 
AAGGCCACCA CCAAGAAGAT ATGTCGCAGT CAAACGCCGC TCTGGAACGA AAGCTTTATG 
CTGGAACTGG AGGGCAGCCA GAATGTACGC ATTCTTCTTT ATGAGGCTAA GGAGCGACCG 
CTTCTTAAAG CCAAGCACAT ACTTAAGTTG AGTTTAAGTT GGCTGACAGA AACAACGCAG 
CCGAAGTCAA TTAAACTTAC TGAGACCTTA GAGCTCGGCT GTAGCTTCCG TTTTATACCA 
GGCGAGCTCT TCCGCGGCAG CACAAAGCCA GGAGCCCTCT TCGGTGCCAA AATGAGTCAA 
GTATTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP1A ( NM_001169155.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162626.1 Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa03009
Clone ID Related Accession (Same CDS sequence)	NM_001297797.1 , NM_001169155.1
Accession Version	NM_001169155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 1A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACCCA GACCTCCGCC AAAGAATATT CGGCGAGTTG AGCCCCCTAC CATTCCCAGC 
CAGCAGCTAT CGTCTTCGCC CAAAAGGGAG ACATATATGA TCAACACGAT GCCAAGCAGT 
TCAAGCGGCA GTCTCTGCAT TGACAGCCTG GGCAATTCGC TGGAGCGGAA CATCAAGCCG 
TCTGATATAC TACGTCAGAA ATCATCTGAT AATTTGGATT CCAAGTACTC GGCAAAACGG 
AAAACCACAC CGGAGCTTAG TAAATTTGTG AACGTAAACA ATCCTGAATT AATGGAGGAG 
CTCGGTCGTA AGATGGTAAG CAAGGCAGAC AGCCTGGAAC AAACGAGGAG GAACGACTCG 
TCGCCTTCTG GCTGTGGTAG CCTAGGACGC ACTTCCTCCA CGCCTGGGCG ATCGTTGACT 
CCTGGACGAA CCGGAGTTGG TGCCTCGCCA ACTGGTAGTC TAAGTAAAAC CGGAAAACTG 
GCAATGGCGG ACAGCAGCTC CACTAGCAGT CTAGAGAGGA GATCGTGCCT GCAGGCTGTC 
GATGCGGACA TTGGAACGGA ATCGCGGGAA TCGGGTGCCA ACCAGCGTAG TGTAGGGTCC 
AAGGAGTCGC TGACATCACA AGGCATATCG GAGGTGATCA AGAGCTACGA AAGCGTGTCT 
TCGCTAAGTT CGGAAAGTGC AAAGGCGGTG GCTTCCCAAC AGTCGGCGGC AGCGGGCGGA 
GCTTTAGCGG AGTGTGAACC GTACTATGAT AGCGTACCGC TCGACAACGG CGACGGAGAA 
TACGTTTTTA TTAAGCCCGG CCGCACCGGA TCCTCATCCA GTCGCGATGA TTTGTCGACG 
CCTGGGAGCA GCATTTTGCC TTTGCCTAAT GTAGCGGATC CCGAATCTCC AGGCAGGACC 
TCTAACTACG TCAACATCGA TTTCTTCCTT CAGCAAGGAA TTGAGACGCG CTCCAGTTCG 
CTAGACAGTG ACGGGGAGTA CGAGGGTCCG CCCATATTAC GCACCATTTC GCACGACGAT 
CAAACCACAA CACAAACTAC TCCGGGAGCA ATTCGCAAGG TTTGTGGAAA TGCGCGCGGT 
GCTAAAATAC GTTCCATACT TAGCTCAATT ATTCAAAGTG AGACTATCTA CGTGGAATGC 
CTCAATAAAA TGATGCAGTA CAAGAAGGCC ATCCACGCAA CCCTAACTAC TTCGCAGCCT 
GTGATCAGGG AGGAGGAGGA GAACACAATT TTCTTTAAAA TTGATGAGCT TTACGATCTG 
CATACCGGTT TCTTATCTGA TTTAAAGACT ATTGTGTCTC ACGAGGGTGG TGATGTGCTG 
ATCGGTGAGC CGTTTCGTCG ACTGGCGGAG ATGTTTGACT TATACAGTGC CTTTTTGCAC 
AACTACAAAA ATGCTATTGA GACTGTGAAG AAGTGCAGTG CCAATAACCC CCAGTTTAAA 
AAGATCGTCT CCACCATTGT GCTTAATCTG CAGACGGAAC AATCGCTAAC TCTCGAGGAT 
CTATTGCACA AACCTGTTGC GAGGGTACAA ATCAACGCAT TGGTCTTCAA CGATTTGCTA 
CGCGAAACCC CAAATGCGCA TCCGGATCAC CAGCCATTAA GGCAGGCGCA AAAGATTATT 
CAGATGTTCC TCAATCAGTT CAATGTGGTC AATCAGCGGT TGCCCATTGA ATCGAATCGA 
AATTTAAGAC GCATGGTGCG TAATTCTTTT ATTGTAGAAC TTGTCGATGG ACATCGTAAG 
CTGCGGCATC TCTTTCTTTT TAATGATGTC ATCGCATGTG CCAAATACAA AGCATTAGGC 
CGCGATCGTA TCGACTATGA GCTCAAATGG TTCATTCCGC TAAAAGACGT ATCCATTTAC 
GAGGAAGCAG ATCCGGCGGT CGAGCTTAAG GAATCTAGTC CAGCAAACAT TTCGCAAGTA 
AAACGCAATT TGCGATCCGT TCGAGATCAA TTGGCCATGG AGGTGGCTAA CAGTGGAAGT 
GGAGCCTTCC GTTCCGGTGA TAAGTATCGC CGCAAGCTGG CGGATCTGGA ATCACAGCTG 
GTGCTGGCAA CGCCCAATTT AGTGTTGCGC CTGGGGAACA AGGCAAATAA TAAAACCATA 
ACCTTCTTTC TTAGCTCGGA TTTTGAGAGG ACGCAGTGGA TCGACTCAAT CTTATCCCTT 
AAGCAAAAAT GCAACCTGCC TGGAGCAAAT ACTATTAATT CCCTGGAGGT TACCGCCTTC 
ATTGTAGCCA TGCAGAAGGG CATGAAAACC GAAATGGGTT CATATCTTAT GCGTAACACT 
AACGACGAGA GCCTGTTGGT TGGGGACCTG TACATGGGAG TTCATGGATT GGAAGGACTG 
GAGCAAGCGA ACGACCTGTA CATCTGCGTC GAGGTGGACT CCTATGGCCA CTATTTTCGC 
AAGGCCACCA CCAAGAAGAT ATGTCGCAGT CAAACGCCGC TCTGGAACGA AAGCTTTATG 
CTGGAACTGG AGGGCAGCCA GAATGTACGC ATTCTTCTTT ATGAGGCTAA GGAGCGACCG 
CTTCTTAAAG CCAAGCACAT ACTTAAGTTG AGTTTAAGTT GGCTGACAGA AACAACGCAG 
CCGAAGTCAA TTAAACTTAC TGAGACCTTA GAGCTCGGCT GTAGCTTCCG TTTTATACCA 
GGCGAGCTCT TCCGCGGCAG CACAAAGCCA GGAGCCCTCT TCGGTGCCAA AATGAGTCAA 
GTATTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162626.1
CDS	1431..4142
Translation	MPPRPPPKNIRRVEPPTIPSQQLSSSPKRETYMINTMPSSSSGSLCIDSLGNSLERNIKPSDILRQKSSDNLDSKYSAKRKTTPELSKFVNVNNPELMEELGRKMVSKADSLEQTRRNDSSPSGCGSLGRTSSTPGRSLTPGRTGVGASPTGSLSKTGKLAMADSSSTSSLERRSCLQAVDADIGTESRESGANQRSVGSKESLTSQGISEVIKSYESVSSLSSESAKAVASQQSAAAGGALAECEPYYDSVPLDNGDGEYVFIKPGRTGSSSSRDDLSTPGSSILPLPNVADPESPGRTSNYVNIDFFLQQGIETRSSSLDSDGEYEGPPILRTISHDDQTTTQTTPGAIRKVCGNARGAKIRSILSSIIQSETIYVECLNKMMQYKKAIHATLTTSQPVIREEEENTIFFKIDELYDLHTGFLSDLKTIVSHEGGDVLIGEPFRRLAEMFDLYSAFLHNYKNAIETVKKCSANNPQFKKIVSTIVLNLQTEQSLTLEDLLHKPVARVQINALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQFNVVNQRLPIESNRNLRRMVRNSFIVELVDGHRKLRHLFLFNDVIACAKYKALGRDRIDYELKWFIPLKDVSIYEEADPAVELKESSPANISQVKRNLRSVRDQLAMEVANSGSGAFRSGDKYRRKLADLESQLVLATPNLVLRLGNKANNKTITFFLSSDFERTQWIDSILSLKQKCNLPGANTINSLEVTAFIVAMQKGMKTEMGSYLMRNTNDESLLVGDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPLWNESFMLELEGSQNVRILLYEAKERPLLKAKHILKLSLSWLTETTQPKSIKLTETLELGCSFRFIPGELFRGSTKPGALFGAKMSQVLK

Target ORF information:

RefSeq Version	NM_001169155.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169155.1

ORF Insert Sequence:

ATGCCACCCA GACCTCCGCC AAAGAATATT CGGCGAGTTG AGCCCCCTAC CATTCCCAGC 
CAGCAGCTAT CGTCTTCGCC CAAAAGGGAG ACATATATGA TCAACACGAT GCCAAGCAGT 
TCAAGCGGCA GTCTCTGCAT TGACAGCCTG GGCAATTCGC TGGAGCGGAA CATCAAGCCG 
TCTGATATAC TACGTCAGAA ATCATCTGAT AATTTGGATT CCAAGTACTC GGCAAAACGG 
AAAACCACAC CGGAGCTTAG TAAATTTGTG AACGTAAACA ATCCTGAATT AATGGAGGAG 
CTCGGTCGTA AGATGGTAAG CAAGGCAGAC AGCCTGGAAC AAACGAGGAG GAACGACTCG 
TCGCCTTCTG GCTGTGGTAG CCTAGGACGC ACTTCCTCCA CGCCTGGGCG ATCGTTGACT 
CCTGGACGAA CCGGAGTTGG TGCCTCGCCA ACTGGTAGTC TAAGTAAAAC CGGAAAACTG 
GCAATGGCGG ACAGCAGCTC CACTAGCAGT CTAGAGAGGA GATCGTGCCT GCAGGCTGTC 
GATGCGGACA TTGGAACGGA ATCGCGGGAA TCGGGTGCCA ACCAGCGTAG TGTAGGGTCC 
AAGGAGTCGC TGACATCACA AGGCATATCG GAGGTGATCA AGAGCTACGA AAGCGTGTCT 
TCGCTAAGTT CGGAAAGTGC AAAGGCGGTG GCTTCCCAAC AGTCGGCGGC AGCGGGCGGA 
GCTTTAGCGG AGTGTGAACC GTACTATGAT AGCGTACCGC TCGACAACGG CGACGGAGAA 
TACGTTTTTA TTAAGCCCGG CCGCACCGGA TCCTCATCCA GTCGCGATGA TTTGTCGACG 
CCTGGGAGCA GCATTTTGCC TTTGCCTAAT GTAGCGGATC CCGAATCTCC AGGCAGGACC 
TCTAACTACG TCAACATCGA TTTCTTCCTT CAGCAAGGAA TTGAGACGCG CTCCAGTTCG 
CTAGACAGTG ACGGGGAGTA CGAGGGTCCG CCCATATTAC GCACCATTTC GCACGACGAT 
CAAACCACAA CACAAACTAC TCCGGGAGCA ATTCGCAAGG TTTGTGGAAA TGCGCGCGGT 
GCTAAAATAC GTTCCATACT TAGCTCAATT ATTCAAAGTG AGACTATCTA CGTGGAATGC 
CTCAATAAAA TGATGCAGTA CAAGAAGGCC ATCCACGCAA CCCTAACTAC TTCGCAGCCT 
GTGATCAGGG AGGAGGAGGA GAACACAATT TTCTTTAAAA TTGATGAGCT TTACGATCTG 
CATACCGGTT TCTTATCTGA TTTAAAGACT ATTGTGTCTC ACGAGGGTGG TGATGTGCTG 
ATCGGTGAGC CGTTTCGTCG ACTGGCGGAG ATGTTTGACT TATACAGTGC CTTTTTGCAC 
AACTACAAAA ATGCTATTGA GACTGTGAAG AAGTGCAGTG CCAATAACCC CCAGTTTAAA 
AAGATCGTCT CCACCATTGT GCTTAATCTG CAGACGGAAC AATCGCTAAC TCTCGAGGAT 
CTATTGCACA AACCTGTTGC GAGGGTACAA ATCAACGCAT TGGTCTTCAA CGATTTGCTA 
CGCGAAACCC CAAATGCGCA TCCGGATCAC CAGCCATTAA GGCAGGCGCA AAAGATTATT 
CAGATGTTCC TCAATCAGTT CAATGTGGTC AATCAGCGGT TGCCCATTGA ATCGAATCGA 
AATTTAAGAC GCATGGTGCG TAATTCTTTT ATTGTAGAAC TTGTCGATGG ACATCGTAAG 
CTGCGGCATC TCTTTCTTTT TAATGATGTC ATCGCATGTG CCAAATACAA AGCATTAGGC 
CGCGATCGTA TCGACTATGA GCTCAAATGG TTCATTCCGC TAAAAGACGT ATCCATTTAC 
GAGGAAGCAG ATCCGGCGGT CGAGCTTAAG GAATCTAGTC CAGCAAACAT TTCGCAAGTA 
AAACGCAATT TGCGATCCGT TCGAGATCAA TTGGCCATGG AGGTGGCTAA CAGTGGAAGT 
GGAGCCTTCC GTTCCGGTGA TAAGTATCGC CGCAAGCTGG CGGATCTGGA ATCACAGCTG 
GTGCTGGCAA CGCCCAATTT AGTGTTGCGC CTGGGGAACA AGGCAAATAA TAAAACCATA 
ACCTTCTTTC TTAGCTCGGA TTTTGAGAGG ACGCAGTGGA TCGACTCAAT CTTATCCCTT 
AAGCAAAAAT GCAACCTGCC TGGAGCAAAT ACTATTAATT CCCTGGAGGT TACCGCCTTC 
ATTGTAGCCA TGCAGAAGGG CATGAAAACC GAAATGGGTT CATATCTTAT GCGTAACACT 
AACGACGAGA GCCTGTTGGT TGGGGACCTG TACATGGGAG TTCATGGATT GGAAGGACTG 
GAGCAAGCGA ACGACCTGTA CATCTGCGTC GAGGTGGACT CCTATGGCCA CTATTTTCGC 
AAGGCCACCA CCAAGAAGAT ATGTCGCAGT CAAACGCCGC TCTGGAACGA AAGCTTTATG 
CTGGAACTGG AGGGCAGCCA GAATGTACGC ATTCTTCTTT ATGAGGCTAA GGAGCGACCG 
CTTCTTAAAG CCAAGCACAT ACTTAAGTTG AGTTTAAGTT GGCTGACAGA AACAACGCAG 
CCGAAGTCAA TTAAACTTAC TGAGACCTTA GAGCTCGGCT GTAGCTTCCG TTTTATACCA 
GGCGAGCTCT TCCGCGGCAG CACAAAGCCA GGAGCCCTCT TCGGTGCCAA AATGAGTCAA 
GTATTAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP1A ( NM_001103385.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096855.1 Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant A, mRNA.

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CloneID	OFa02623
Clone ID Related Accession (Same CDS sequence)	NM_001103385.2 , NM_001103386.2
Accession Version	NM_001103385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 1A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_001103385.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGGTGA TCAAGAGCTA CGAAAGCGTG 
TCTTCGCTAA GTTCGGAAAG TGCAAAGGCG GTGGCTTCCC AACAGTCGGC GGCAGCGGGC 
GGAGCTTTAG CGGAGTGTGA ACCGTACTAT GATAGCGTAC CGCTCGACAA CGGCGACGGA 
GAATACGTTT TTATTAAGCC CGGCCGCACC GGATCCTCAT CCAGTCGCGA TGATTTGTCG 
ACGCCTGGGA GCAGCATTTT GCCTTTGCCT AATGTAGCGG ATCCCGAATC TCCAGGCAGG 
ACCTCTAACT ACGTCAACAT CGATTTCTTC CTTCAGCAAG GAATTGAGAC GCGCTCCAGT 
TCGCTAGACA GTGACGGGGA GTACGAGGGT CCGCCCATAT TACGCACCAT TTCGCACGAC 
GATCAAACCA CAACACAAAC TACTCCGGGA GCAATTCGCA AGAACTTAGT TTCGGCGATA 
CTTGTTTGTG GAAATGCGCG CGGTGCTAAA ATACGTTCCA TACTTAGCTC AATTATTCAA 
AGTGAGACTA TCTACGTGGA ATGCCTCAAT AAAATGATGC AGTACAAGAA GGCCATCCAC 
GCAACCCTAA CTACTTCGCA GCCTGTGATC AGGGAGGAGG AGGAGAACAC AATTTTCTTT 
AAAATTGATG AGCTTTACGA TCTGCATACC GGTTTCTTAT CTGATTTAAA GACTATTGTG 
TCTCACGAGG GTGGTGATGT GCTGATCGGT GAGCCGTTTC GTCGACTGGC GGAGATGTTT 
GACTTATACA GTGCCTTTTT GCACAACTAC AAAAATGCTA TTGAGACTGT GAAGAAGTGC 
AGTGCCAATA ACCCCCAGTT TAAAAAGATC GTCTCCACCA TTGTGCTTAA TCTGCAGACG 
GAACAATCGC TAACTCTCGA GGATCTATTG CACAAACCTG TTGCGAGGGT ACAAATCAAC 
GCATTGGTCT TCAACGATTT GCTACGCGAA ACCCCAAATG CGCATCCGGA TCACCAGCCA 
TTAAGGCAGG CGCAAAAGAT TATTCAGATG TTCCTCAATC AGTTCAATGT GGTCAATCAG 
CGGTTGCCCA TTGAATCGAA TCGAAATTTA AGACGCATGG TGCGTAATTC TTTTATTGTA 
GAACTTGTCG ATGGACATCG TAAGCTGCGG CATCTCTTTC TTTTTAATGA TGTCATCGCA 
TGTGCCAAAT ACAAAGCATT AGGCCGCGAT CGTATCGACT ATGAGCTCAA ATGGTTCATT 
CCGCTAAAAG ACGTATCCAT TTACGAGGAA GCAGATCCGG CGGTCGAGCT TAAGGAATCT 
AGTCCAGCAA ACATTTCGCA AGTAAAACGC AATTTGCGAT CCGTTCGAGA TCAATTGGCC 
ATGGAGGTGG CTAACAGTGG AAGTGGAGCC TTCCGTTCCG GTGATAAGTA TCGCCGCAAG 
CTGGCGGATC TGGAATCACA GCTGGTGCTG GCAACGCCCA ATTTAGTGTT GCGCCTGGGG 
AACAAGGCAA ATAATAAAAC CATAACCTTC TTTCTTAGCT CGGATTTTGA GAGGACGCAG 
TGGATCGACT CAATCTTATC CCTTAAGCAA AAATGCAACC TGCCTGGAGC AAATACTATT 
AATTCCCTGG AGGTTACCGC CTTCATTGTA GCCATGCAGA AGGGCATGAA AACCGAAATG 
GGTTCATATC TTATGCGTAA CACTAACGAC GAGAGCCTGT TGGTTGGGGA CCTGTACATG 
GGAGTTCATG GATTGGAAGG ACTGGAGCAA GCGAACGACC TGTACATCTG CGTCGAGGTG 
GACTCCTATG GCCACTATTT TCGCAAGGCC ACCACCAAGA AGATATGTCG CAGTCAAACG 
CCGCTCTGGA ACGAAAGCTT TATGCTGGAA CTGGAGGGCA GCCAGAATGT ACGCATTCTT 
CTTTATGAGG CTAAGGAGCG ACCGCTTCTT AAAGCCAAGC ACATACTTAA GTTGAGTTTA 
AGTTGGCTGA CAGAAACAAC GCAGCCGAAG TCAATTAAAC TTACTGAGAC CTTAGAGCTC 
GGCTGTAGCT TCCGTTTTAT ACCAGGCGAG CTCTTCCGCG GCAGCACAAA GCCAGGAGCC 
CTCTTCGGTG CCAAAATGAG TCAAGTATTA AAACGTGAAA AACGCGACAT TCCGTTCATC 
ATAGGCGCCT GCATTCGGGA GGTAGAGCGG CGTGGGATGC TGGAGGTTGG CTGCTACCGC 
GTCAGCGGTT CCGCTTCTGA TTTGGCGAAG CTGAAGAAGG CCTTTGAGTC TGATGCCTAT 
GAGGCGGAGC AGTTGCTACG CGAGGTGGAC ATCCATTCGG TCACTGGCAT TCTGAAAACT 
TTTTTAAGGG AGCTCCCCGA GGCCCTTTTC ACGGATCAAC TTTATCCACG ATTTTTCGAC 
ACATTCAGCG CCTTTAGCAA CAACAACGAA TCCACACGAA TCAACGAGCT TCTAAAAGTT 
TTTGAGGAGT TGCCACAGGC CAACAAAGCC TCCATTACTT CGATATTAGA TCATTTAATA 
AGAGTCCACG AAAAGGAGAC AGATAACAAG ATGTCGTTGC ACAATCTGGC CATGGTGTTT 
GGACCAACCT TGCTAAGGCC GGGCCAAACG CAGGTGAAGC AGAAGGATCC ACTGGCAGCC 
AGCACAGTCG ACGTTATGGC TCAGGCAGGA ATCCTATACT GCTTTCTTCA GGCACGGATC 
AAGAAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096855.1
CDS	305..4516
Translation	MSVFDDFQRLWMQRFPQSSLSDAWEQDVRASLERHKVRINELSKELEQETLYVEYLERLLSDVERYRVSGGDPKALFEAASGSCASSNNSNCSSTTNGATQEESNTNLDKDFKSSLNLRESPSSTGAKDKDRLSLNPDNKYTTHTNAVIGGIGSEKVSGPLHSTQITADGKRTGELYLKREEDKCKDSNSEDTDGALQQCITELAASLKAQPDNVAGGRLENDVDIDKPLRKSTSQYVTVIQVKEAKDKESADGTTSSSGIGEPQQQPTPPSTFSRYSEATAPPLSPSLTSYAAHVEAKKKMPPRPPPKNIRRVEPPTIPSQQLSSSPKRETYMINTMPSSSSGSLCIDSLGNSLERNIKPSDILRQKSSDNLDSKYSAKRKTTPELSKFVNVNNPELMEELGRKMVSKADSLEQTRRNDSSPSGCGSLGRTSSTPGRSLTPGRTGVGASPTGSLSKTGKLAMADSSSTSSLERRSCLQAVDADIGTESRESGANQRSVGSKESLTSQGISEVIKSYESVSSLSSESAKAVASQQSAAAGGALAECEPYYDSVPLDNGDGEYVFIKPGRTGSSSSRDDLSTPGSSILPLPNVADPESPGRTSNYVNIDFFLQQGIETRSSSLDSDGEYEGPPILRTISHDDQTTTQTTPGAIRKNLVSAILVCGNARGAKIRSILSSIIQSETIYVECLNKMMQYKKAIHATLTTSQPVIREEEENTIFFKIDELYDLHTGFLSDLKTIVSHEGGDVLIGEPFRRLAEMFDLYSAFLHNYKNAIETVKKCSANNPQFKKIVSTIVLNLQTEQSLTLEDLLHKPVARVQINALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQFNVVNQRLPIESNRNLRRMVRNSFIVELVDGHRKLRHLFLFNDVIACAKYKALGRDRIDYELKWFIPLKDVSIYEEADPAVELKESSPANISQVKRNLRSVRDQLAMEVANSGSGAFRSGDKYRRKLADLESQLVLATPNLVLRLGNKANNKTITFFLSSDFERTQWIDSILSLKQKCNLPGANTINSLEVTAFIVAMQKGMKTEMGSYLMRNTNDESLLVGDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPLWNESFMLELEGSQNVRILLYEAKERPLLKAKHILKLSLSWLTETTQPKSIKLTETLELGCSFRFIPGELFRGSTKPGALFGAKMSQVLKREKRDIPFIIGACIREVERRGMLEVGCYRVSGSASDLAKLKKAFESDAYEAEQLLREVDIHSVTGILKTFLRELPEALFTDQLYPRFFDTFSAFSNNNESTRINELLKVFEELPQANKASITSILDHLIRVHEKETDNKMSLHNLAMVFGPTLLRPGQTQVKQKDPLAASTVDVMAQAGILYCFLQARIKKD

Target ORF information:

RefSeq Version	NM_001103385.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103385.2

ORF Insert Sequence:

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGGTGA TCAAGAGCTA CGAAAGCGTG 
TCTTCGCTAA GTTCGGAAAG TGCAAAGGCG GTGGCTTCCC AACAGTCGGC GGCAGCGGGC 
GGAGCTTTAG CGGAGTGTGA ACCGTACTAT GATAGCGTAC CGCTCGACAA CGGCGACGGA 
GAATACGTTT TTATTAAGCC CGGCCGCACC GGATCCTCAT CCAGTCGCGA TGATTTGTCG 
ACGCCTGGGA GCAGCATTTT GCCTTTGCCT AATGTAGCGG ATCCCGAATC TCCAGGCAGG 
ACCTCTAACT ACGTCAACAT CGATTTCTTC CTTCAGCAAG GAATTGAGAC GCGCTCCAGT 
TCGCTAGACA GTGACGGGGA GTACGAGGGT CCGCCCATAT TACGCACCAT TTCGCACGAC 
GATCAAACCA CAACACAAAC TACTCCGGGA GCAATTCGCA AGAACTTAGT TTCGGCGATA 
CTTGTTTGTG GAAATGCGCG CGGTGCTAAA ATACGTTCCA TACTTAGCTC AATTATTCAA 
AGTGAGACTA TCTACGTGGA ATGCCTCAAT AAAATGATGC AGTACAAGAA GGCCATCCAC 
GCAACCCTAA CTACTTCGCA GCCTGTGATC AGGGAGGAGG AGGAGAACAC AATTTTCTTT 
AAAATTGATG AGCTTTACGA TCTGCATACC GGTTTCTTAT CTGATTTAAA GACTATTGTG 
TCTCACGAGG GTGGTGATGT GCTGATCGGT GAGCCGTTTC GTCGACTGGC GGAGATGTTT 
GACTTATACA GTGCCTTTTT GCACAACTAC AAAAATGCTA TTGAGACTGT GAAGAAGTGC 
AGTGCCAATA ACCCCCAGTT TAAAAAGATC GTCTCCACCA TTGTGCTTAA TCTGCAGACG 
GAACAATCGC TAACTCTCGA GGATCTATTG CACAAACCTG TTGCGAGGGT ACAAATCAAC 
GCATTGGTCT TCAACGATTT GCTACGCGAA ACCCCAAATG CGCATCCGGA TCACCAGCCA 
TTAAGGCAGG CGCAAAAGAT TATTCAGATG TTCCTCAATC AGTTCAATGT GGTCAATCAG 
CGGTTGCCCA TTGAATCGAA TCGAAATTTA AGACGCATGG TGCGTAATTC TTTTATTGTA 
GAACTTGTCG ATGGACATCG TAAGCTGCGG CATCTCTTTC TTTTTAATGA TGTCATCGCA 
TGTGCCAAAT ACAAAGCATT AGGCCGCGAT CGTATCGACT ATGAGCTCAA ATGGTTCATT 
CCGCTAAAAG ACGTATCCAT TTACGAGGAA GCAGATCCGG CGGTCGAGCT TAAGGAATCT 
AGTCCAGCAA ACATTTCGCA AGTAAAACGC AATTTGCGAT CCGTTCGAGA TCAATTGGCC 
ATGGAGGTGG CTAACAGTGG AAGTGGAGCC TTCCGTTCCG GTGATAAGTA TCGCCGCAAG 
CTGGCGGATC TGGAATCACA GCTGGTGCTG GCAACGCCCA ATTTAGTGTT GCGCCTGGGG 
AACAAGGCAA ATAATAAAAC CATAACCTTC TTTCTTAGCT CGGATTTTGA GAGGACGCAG 
TGGATCGACT CAATCTTATC CCTTAAGCAA AAATGCAACC TGCCTGGAGC AAATACTATT 
AATTCCCTGG AGGTTACCGC CTTCATTGTA GCCATGCAGA AGGGCATGAA AACCGAAATG 
GGTTCATATC TTATGCGTAA CACTAACGAC GAGAGCCTGT TGGTTGGGGA CCTGTACATG 
GGAGTTCATG GATTGGAAGG ACTGGAGCAA GCGAACGACC TGTACATCTG CGTCGAGGTG 
GACTCCTATG GCCACTATTT TCGCAAGGCC ACCACCAAGA AGATATGTCG CAGTCAAACG 
CCGCTCTGGA ACGAAAGCTT TATGCTGGAA CTGGAGGGCA GCCAGAATGT ACGCATTCTT 
CTTTATGAGG CTAAGGAGCG ACCGCTTCTT AAAGCCAAGC ACATACTTAA GTTGAGTTTA 
AGTTGGCTGA CAGAAACAAC GCAGCCGAAG TCAATTAAAC TTACTGAGAC CTTAGAGCTC 
GGCTGTAGCT TCCGTTTTAT ACCAGGCGAG CTCTTCCGCG GCAGCACAAA GCCAGGAGCC 
CTCTTCGGTG CCAAAATGAG TCAAGTATTA AAACGTGAAA AACGCGACAT TCCGTTCATC 
ATAGGCGCCT GCATTCGGGA GGTAGAGCGG CGTGGGATGC TGGAGGTTGG CTGCTACCGC 
GTCAGCGGTT CCGCTTCTGA TTTGGCGAAG CTGAAGAAGG CCTTTGAGTC TGATGCCTAT 
GAGGCGGAGC AGTTGCTACG CGAGGTGGAC ATCCATTCGG TCACTGGCAT TCTGAAAACT 
TTTTTAAGGG AGCTCCCCGA GGCCCTTTTC ACGGATCAAC TTTATCCACG ATTTTTCGAC 
ACATTCAGCG CCTTTAGCAA CAACAACGAA TCCACACGAA TCAACGAGCT TCTAAAAGTT 
TTTGAGGAGT TGCCACAGGC CAACAAAGCC TCCATTACTT CGATATTAGA TCATTTAATA 
AGAGTCCACG AAAAGGAGAC AGATAACAAG ATGTCGTTGC ACAATCTGGC CATGGTGTTT 
GGACCAACCT TGCTAAGGCC GGGCCAAACG CAGGTGAAGC AGAAGGATCC ACTGGCAGCC 
AGCACAGTCG ACGTTATGGC TCAGGCAGGA ATCCTATACT GCTTTCTTCA GGCACGGATC 
AAGAAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP1A ( NM_001103386.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096856.1 Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02623
Clone ID Related Accession (Same CDS sequence)	NM_001103385.2 , NM_001103386.2
Accession Version	NM_001103386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 1A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_001103386.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGGTGA TCAAGAGCTA CGAAAGCGTG 
TCTTCGCTAA GTTCGGAAAG TGCAAAGGCG GTGGCTTCCC AACAGTCGGC GGCAGCGGGC 
GGAGCTTTAG CGGAGTGTGA ACCGTACTAT GATAGCGTAC CGCTCGACAA CGGCGACGGA 
GAATACGTTT TTATTAAGCC CGGCCGCACC GGATCCTCAT CCAGTCGCGA TGATTTGTCG 
ACGCCTGGGA GCAGCATTTT GCCTTTGCCT AATGTAGCGG ATCCCGAATC TCCAGGCAGG 
ACCTCTAACT ACGTCAACAT CGATTTCTTC CTTCAGCAAG GAATTGAGAC GCGCTCCAGT 
TCGCTAGACA GTGACGGGGA GTACGAGGGT CCGCCCATAT TACGCACCAT TTCGCACGAC 
GATCAAACCA CAACACAAAC TACTCCGGGA GCAATTCGCA AGAACTTAGT TTCGGCGATA 
CTTGTTTGTG GAAATGCGCG CGGTGCTAAA ATACGTTCCA TACTTAGCTC AATTATTCAA 
AGTGAGACTA TCTACGTGGA ATGCCTCAAT AAAATGATGC AGTACAAGAA GGCCATCCAC 
GCAACCCTAA CTACTTCGCA GCCTGTGATC AGGGAGGAGG AGGAGAACAC AATTTTCTTT 
AAAATTGATG AGCTTTACGA TCTGCATACC GGTTTCTTAT CTGATTTAAA GACTATTGTG 
TCTCACGAGG GTGGTGATGT GCTGATCGGT GAGCCGTTTC GTCGACTGGC GGAGATGTTT 
GACTTATACA GTGCCTTTTT GCACAACTAC AAAAATGCTA TTGAGACTGT GAAGAAGTGC 
AGTGCCAATA ACCCCCAGTT TAAAAAGATC GTCTCCACCA TTGTGCTTAA TCTGCAGACG 
GAACAATCGC TAACTCTCGA GGATCTATTG CACAAACCTG TTGCGAGGGT ACAAATCAAC 
GCATTGGTCT TCAACGATTT GCTACGCGAA ACCCCAAATG CGCATCCGGA TCACCAGCCA 
TTAAGGCAGG CGCAAAAGAT TATTCAGATG TTCCTCAATC AGTTCAATGT GGTCAATCAG 
CGGTTGCCCA TTGAATCGAA TCGAAATTTA AGACGCATGG TGCGTAATTC TTTTATTGTA 
GAACTTGTCG ATGGACATCG TAAGCTGCGG CATCTCTTTC TTTTTAATGA TGTCATCGCA 
TGTGCCAAAT ACAAAGCATT AGGCCGCGAT CGTATCGACT ATGAGCTCAA ATGGTTCATT 
CCGCTAAAAG ACGTATCCAT TTACGAGGAA GCAGATCCGG CGGTCGAGCT TAAGGAATCT 
AGTCCAGCAA ACATTTCGCA AGTAAAACGC AATTTGCGAT CCGTTCGAGA TCAATTGGCC 
ATGGAGGTGG CTAACAGTGG AAGTGGAGCC TTCCGTTCCG GTGATAAGTA TCGCCGCAAG 
CTGGCGGATC TGGAATCACA GCTGGTGCTG GCAACGCCCA ATTTAGTGTT GCGCCTGGGG 
AACAAGGCAA ATAATAAAAC CATAACCTTC TTTCTTAGCT CGGATTTTGA GAGGACGCAG 
TGGATCGACT CAATCTTATC CCTTAAGCAA AAATGCAACC TGCCTGGAGC AAATACTATT 
AATTCCCTGG AGGTTACCGC CTTCATTGTA GCCATGCAGA AGGGCATGAA AACCGAAATG 
GGTTCATATC TTATGCGTAA CACTAACGAC GAGAGCCTGT TGGTTGGGGA CCTGTACATG 
GGAGTTCATG GATTGGAAGG ACTGGAGCAA GCGAACGACC TGTACATCTG CGTCGAGGTG 
GACTCCTATG GCCACTATTT TCGCAAGGCC ACCACCAAGA AGATATGTCG CAGTCAAACG 
CCGCTCTGGA ACGAAAGCTT TATGCTGGAA CTGGAGGGCA GCCAGAATGT ACGCATTCTT 
CTTTATGAGG CTAAGGAGCG ACCGCTTCTT AAAGCCAAGC ACATACTTAA GTTGAGTTTA 
AGTTGGCTGA CAGAAACAAC GCAGCCGAAG TCAATTAAAC TTACTGAGAC CTTAGAGCTC 
GGCTGTAGCT TCCGTTTTAT ACCAGGCGAG CTCTTCCGCG GCAGCACAAA GCCAGGAGCC 
CTCTTCGGTG CCAAAATGAG TCAAGTATTA AAACGTGAAA AACGCGACAT TCCGTTCATC 
ATAGGCGCCT GCATTCGGGA GGTAGAGCGG CGTGGGATGC TGGAGGTTGG CTGCTACCGC 
GTCAGCGGTT CCGCTTCTGA TTTGGCGAAG CTGAAGAAGG CCTTTGAGTC TGATGCCTAT 
GAGGCGGAGC AGTTGCTACG CGAGGTGGAC ATCCATTCGG TCACTGGCAT TCTGAAAACT 
TTTTTAAGGG AGCTCCCCGA GGCCCTTTTC ACGGATCAAC TTTATCCACG ATTTTTCGAC 
ACATTCAGCG CCTTTAGCAA CAACAACGAA TCCACACGAA TCAACGAGCT TCTAAAAGTT 
TTTGAGGAGT TGCCACAGGC CAACAAAGCC TCCATTACTT CGATATTAGA TCATTTAATA 
AGAGTCCACG AAAAGGAGAC AGATAACAAG ATGTCGTTGC ACAATCTGGC CATGGTGTTT 
GGACCAACCT TGCTAAGGCC GGGCCAAACG CAGGTGAAGC AGAAGGATCC ACTGGCAGCC 
AGCACAGTCG ACGTTATGGC TCAGGCAGGA ATCCTATACT GCTTTCTTCA GGCACGGATC 
AAGAAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096856.1
CDS	305..4516
Translation	MSVFDDFQRLWMQRFPQSSLSDAWEQDVRASLERHKVRINELSKELEQETLYVEYLERLLSDVERYRVSGGDPKALFEAASGSCASSNNSNCSSTTNGATQEESNTNLDKDFKSSLNLRESPSSTGAKDKDRLSLNPDNKYTTHTNAVIGGIGSEKVSGPLHSTQITADGKRTGELYLKREEDKCKDSNSEDTDGALQQCITELAASLKAQPDNVAGGRLENDVDIDKPLRKSTSQYVTVIQVKEAKDKESADGTTSSSGIGEPQQQPTPPSTFSRYSEATAPPLSPSLTSYAAHVEAKKKMPPRPPPKNIRRVEPPTIPSQQLSSSPKRETYMINTMPSSSSGSLCIDSLGNSLERNIKPSDILRQKSSDNLDSKYSAKRKTTPELSKFVNVNNPELMEELGRKMVSKADSLEQTRRNDSSPSGCGSLGRTSSTPGRSLTPGRTGVGASPTGSLSKTGKLAMADSSSTSSLERRSCLQAVDADIGTESRESGANQRSVGSKESLTSQGISEVIKSYESVSSLSSESAKAVASQQSAAAGGALAECEPYYDSVPLDNGDGEYVFIKPGRTGSSSSRDDLSTPGSSILPLPNVADPESPGRTSNYVNIDFFLQQGIETRSSSLDSDGEYEGPPILRTISHDDQTTTQTTPGAIRKNLVSAILVCGNARGAKIRSILSSIIQSETIYVECLNKMMQYKKAIHATLTTSQPVIREEEENTIFFKIDELYDLHTGFLSDLKTIVSHEGGDVLIGEPFRRLAEMFDLYSAFLHNYKNAIETVKKCSANNPQFKKIVSTIVLNLQTEQSLTLEDLLHKPVARVQINALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQFNVVNQRLPIESNRNLRRMVRNSFIVELVDGHRKLRHLFLFNDVIACAKYKALGRDRIDYELKWFIPLKDVSIYEEADPAVELKESSPANISQVKRNLRSVRDQLAMEVANSGSGAFRSGDKYRRKLADLESQLVLATPNLVLRLGNKANNKTITFFLSSDFERTQWIDSILSLKQKCNLPGANTINSLEVTAFIVAMQKGMKTEMGSYLMRNTNDESLLVGDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPLWNESFMLELEGSQNVRILLYEAKERPLLKAKHILKLSLSWLTETTQPKSIKLTETLELGCSFRFIPGELFRGSTKPGALFGAKMSQVLKREKRDIPFIIGACIREVERRGMLEVGCYRVSGSASDLAKLKKAFESDAYEAEQLLREVDIHSVTGILKTFLRELPEALFTDQLYPRFFDTFSAFSNNNESTRINELLKVFEELPQANKASITSILDHLIRVHEKETDNKMSLHNLAMVFGPTLLRPGQTQVKQKDPLAASTVDVMAQAGILYCFLQARIKKD

Target ORF information:

RefSeq Version	NM_001103386.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103386.2

ORF Insert Sequence:

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGGTGA TCAAGAGCTA CGAAAGCGTG 
TCTTCGCTAA GTTCGGAAAG TGCAAAGGCG GTGGCTTCCC AACAGTCGGC GGCAGCGGGC 
GGAGCTTTAG CGGAGTGTGA ACCGTACTAT GATAGCGTAC CGCTCGACAA CGGCGACGGA 
GAATACGTTT TTATTAAGCC CGGCCGCACC GGATCCTCAT CCAGTCGCGA TGATTTGTCG 
ACGCCTGGGA GCAGCATTTT GCCTTTGCCT AATGTAGCGG ATCCCGAATC TCCAGGCAGG 
ACCTCTAACT ACGTCAACAT CGATTTCTTC CTTCAGCAAG GAATTGAGAC GCGCTCCAGT 
TCGCTAGACA GTGACGGGGA GTACGAGGGT CCGCCCATAT TACGCACCAT TTCGCACGAC 
GATCAAACCA CAACACAAAC TACTCCGGGA GCAATTCGCA AGAACTTAGT TTCGGCGATA 
CTTGTTTGTG GAAATGCGCG CGGTGCTAAA ATACGTTCCA TACTTAGCTC AATTATTCAA 
AGTGAGACTA TCTACGTGGA ATGCCTCAAT AAAATGATGC AGTACAAGAA GGCCATCCAC 
GCAACCCTAA CTACTTCGCA GCCTGTGATC AGGGAGGAGG AGGAGAACAC AATTTTCTTT 
AAAATTGATG AGCTTTACGA TCTGCATACC GGTTTCTTAT CTGATTTAAA GACTATTGTG 
TCTCACGAGG GTGGTGATGT GCTGATCGGT GAGCCGTTTC GTCGACTGGC GGAGATGTTT 
GACTTATACA GTGCCTTTTT GCACAACTAC AAAAATGCTA TTGAGACTGT GAAGAAGTGC 
AGTGCCAATA ACCCCCAGTT TAAAAAGATC GTCTCCACCA TTGTGCTTAA TCTGCAGACG 
GAACAATCGC TAACTCTCGA GGATCTATTG CACAAACCTG TTGCGAGGGT ACAAATCAAC 
GCATTGGTCT TCAACGATTT GCTACGCGAA ACCCCAAATG CGCATCCGGA TCACCAGCCA 
TTAAGGCAGG CGCAAAAGAT TATTCAGATG TTCCTCAATC AGTTCAATGT GGTCAATCAG 
CGGTTGCCCA TTGAATCGAA TCGAAATTTA AGACGCATGG TGCGTAATTC TTTTATTGTA 
GAACTTGTCG ATGGACATCG TAAGCTGCGG CATCTCTTTC TTTTTAATGA TGTCATCGCA 
TGTGCCAAAT ACAAAGCATT AGGCCGCGAT CGTATCGACT ATGAGCTCAA ATGGTTCATT 
CCGCTAAAAG ACGTATCCAT TTACGAGGAA GCAGATCCGG CGGTCGAGCT TAAGGAATCT 
AGTCCAGCAA ACATTTCGCA AGTAAAACGC AATTTGCGAT CCGTTCGAGA TCAATTGGCC 
ATGGAGGTGG CTAACAGTGG AAGTGGAGCC TTCCGTTCCG GTGATAAGTA TCGCCGCAAG 
CTGGCGGATC TGGAATCACA GCTGGTGCTG GCAACGCCCA ATTTAGTGTT GCGCCTGGGG 
AACAAGGCAA ATAATAAAAC CATAACCTTC TTTCTTAGCT CGGATTTTGA GAGGACGCAG 
TGGATCGACT CAATCTTATC CCTTAAGCAA AAATGCAACC TGCCTGGAGC AAATACTATT 
AATTCCCTGG AGGTTACCGC CTTCATTGTA GCCATGCAGA AGGGCATGAA AACCGAAATG 
GGTTCATATC TTATGCGTAA CACTAACGAC GAGAGCCTGT TGGTTGGGGA CCTGTACATG 
GGAGTTCATG GATTGGAAGG ACTGGAGCAA GCGAACGACC TGTACATCTG CGTCGAGGTG 
GACTCCTATG GCCACTATTT TCGCAAGGCC ACCACCAAGA AGATATGTCG CAGTCAAACG 
CCGCTCTGGA ACGAAAGCTT TATGCTGGAA CTGGAGGGCA GCCAGAATGT ACGCATTCTT 
CTTTATGAGG CTAAGGAGCG ACCGCTTCTT AAAGCCAAGC ACATACTTAA GTTGAGTTTA 
AGTTGGCTGA CAGAAACAAC GCAGCCGAAG TCAATTAAAC TTACTGAGAC CTTAGAGCTC 
GGCTGTAGCT TCCGTTTTAT ACCAGGCGAG CTCTTCCGCG GCAGCACAAA GCCAGGAGCC 
CTCTTCGGTG CCAAAATGAG TCAAGTATTA AAACGTGAAA AACGCGACAT TCCGTTCATC 
ATAGGCGCCT GCATTCGGGA GGTAGAGCGG CGTGGGATGC TGGAGGTTGG CTGCTACCGC 
GTCAGCGGTT CCGCTTCTGA TTTGGCGAAG CTGAAGAAGG CCTTTGAGTC TGATGCCTAT 
GAGGCGGAGC AGTTGCTACG CGAGGTGGAC ATCCATTCGG TCACTGGCAT TCTGAAAACT 
TTTTTAAGGG AGCTCCCCGA GGCCCTTTTC ACGGATCAAC TTTATCCACG ATTTTTCGAC 
ACATTCAGCG CCTTTAGCAA CAACAACGAA TCCACACGAA TCAACGAGCT TCTAAAAGTT 
TTTGAGGAGT TGCCACAGGC CAACAAAGCC TCCATTACTT CGATATTAGA TCATTTAATA 
AGAGTCCACG AAAAGGAGAC AGATAACAAG ATGTCGTTGC ACAATCTGGC CATGGTGTTT 
GGACCAACCT TGCTAAGGCC GGGCCAAACG CAGGTGAAGC AGAAGGATCC ACTGGCAGCC 
AGCACAGTCG ACGTTATGGC TCAGGCAGGA ATCCTATACT GCTTTCTTCA GGCACGGATC 
AAGAAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP1A ( NM_001297796.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284725.1 Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant E, mRNA.

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CloneID	OFa22406
Clone ID Related Accession (Same CDS sequence)	NM_001297796.1
Accession Version	NM_001297796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 1A, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGGTGC AGATCAAGAG CTACGAAAGC 
GTGTCTTCGC TAAGTTCGGA AAGTGCAAAG GCGGTGGCTT CCCAACAGTC GGCGGCAGCG 
GGCGGAGCTT TAGCGGAGTG TGAACCGTAC TATGATAGCG TACCGCTCGA CAACGGCGAC 
GGAGAATACG TTTTTATTAA GCCCGGCCGC ACCGGATCCT CATCCAGTCG CGATGATTTG 
TCGACGCCTG GGAGCAGCAT TTTGCCTTTG CCTAATGTAG CGGATCCCGA ATCTCCAGGC 
AGGACCTCTA ACTACGTCAA CATCGATTTC TTCCTTCAGC AAGGAATTGA GACGCGCTCC 
AGTTCGCTAG ACAGTGACGG GGAGTACGAG GGTCCGCCCA TATTACGCAC CATTTCGCAC 
GACGATCAAA CCACAACACA AACTACTCCG GGAGCAATTC GCAAGAACTT AGTTTCGGCG 
ATACTTGTTT GTGGAAATGC GCGCGGTGCT AAAATACGTT CCATACTTAG CTCAATTATT 
CAAAGTGAGA CTATCTACGT GGAATGCCTC AATAAAATGA TGCAGTACAA GAAGGCCATC 
CACGCAACCC TAACTACTTC GCAGCCTGTG ATCAGGGAGG AGGAGGAGAA CACAATTTTC 
TTTAAAATTG ATGAGCTTTA CGATCTGCAT ACCGGTTTCT TATCTGATTT AAAGACTATT 
GTGTCTCACG AGGGTGGTGA TGTGCTGATC GGTGAGCCGT TTCGTCGACT GGCGGAGATG 
TTTGACTTAT ACAGTGCCTT TTTGCACAAC TACAAAAATG CTATTGAGAC TGTGAAGAAG 
TGCAGTGCCA ATAACCCCCA GTTTAAAAAG ATCGTCTCCA CCATTGTGCT TAATCTGCAG 
ACGGAACAAT CGCTAACTCT CGAGGATCTA TTGCACAAAC CTGTTGCGAG GGTACAAATC 
AACGCATTGG TCTTCAACGA TTTGCTACGC GAAACCCCAA ATGCGCATCC GGATCACCAG 
CCATTAAGGC AGGCGCAAAA GATTATTCAG ATGTTCCTCA ATCAGTTCAA TGTGGTCAAT 
CAGCGGTTGC CCATTGAATC GAATCGAAAT TTAAGACGCA TGGTGCGTAA TTCTTTTATT 
GTAGAACTTG TCGATGGACA TCGTAAGCTG CGGCATCTCT TTCTTTTTAA TGATGTCATC 
GCATGTGCCA AATACAAAGC ATTAGGCCGC GATCGTATCG ACTATGAGCT CAAATGGTTC 
ATTCCGCTAA AAGACGTATC CATTTACGAG GAAGCAGATC CGGCGGTCGA GCTTAAGGAA 
TCTAGTCCAG CAAACATTTC GCAAGTAAAA CGCAATTTGC GATCCGTTCG AGATCAATTG 
GCCATGGAGG TGGCTAACAG TGGAAGTGGA GCCTTCCGTT CCGGTGATAA GTATCGCCGC 
AAGCTGGCGG ATCTGGAATC ACAGCTGGTG CTGGCAACGC CCAATTTAGT GTTGCGCCTG 
GGGAACAAGG CAAATAATAA AACCATAACC TTCTTTCTTA GCTCGGATTT TGAGAGGACG 
CAGTGGATCG ACTCAATCTT ATCCCTTAAG CAAAAATGCA ACCTGCCTGG AGCAAATACT 
ATTAATTCCC TGGAGGTTAC CGCCTTCATT GTAGCCATGC AGAAGGGCAT GAAAACCGAA 
ATGGGTTCAT ATCTTATGCG TAACACTAAC GACGAGAGCC TGTTGGTTGG GGACCTGTAC 
ATGGGAGTTC ATGGATTGGA AGGACTGGAG CAAGCGAACG ACCTGTACAT CTGCGTCGAG 
GTGGACTCCT ATGGCCACTA TTTTCGCAAG GCCACCACCA AGAAGATATG TCGCAGTCAA 
ACGCCGCTCT GGAACGAAAG CTTTATGCTG GAACTGGAGG GCAGCCAGAA TGTACGCATT 
CTTCTTTATG AGGCTAAGGA GCGACCGCTT CTTAAAGCCA AGCACATACT TAAGTTGAGT 
TTAAGTTGGC TGACAGAAAC AACGCAGCCG AAGTCAATTA AACTTACTGA GACCTTAGAG 
CTCGGCTGTA GCTTCCGTTT TATACCAGGC GAGCTCTTCC GCGGCAGCAC AAAGCCAGGA 
GCCCTCTTCG GTGCCAAAAT GAGTCAAGTA TTAAAACGTG AAAAACGCGA CATTCCGTTC 
ATCATAGGCG CCTGCATTCG GGAGGTAGAG CGGCGTGGGA TGCTGGAGGT TGGCTGCTAC 
CGCGTCAGCG GTTCCGCTTC TGATTTGGCG AAGCTGAAGA AGGCCTTTGA GTCTGATGCC 
TATGAGGCGG AGCAGTTGCT ACGCGAGGTG GACATCCATT CGGTCACTGG CATTCTGAAA 
ACTTTTTTAA GGGAGCTCCC CGAGGCCCTT TTCACGGATC AACTTTATCC ACGATTTTTC 
GACACATTCA GCGCCTTTAG CAACAACAAC GAATCCACAC GAATCAACGA GCTTCTAAAA 
GTTTTTGAGG AGTTGCCACA GGCCAACAAA GCCTCCATTA CTTCGATATT AGATCATTTA 
ATAAGAGTCC ACGAAAAGGA GACAGATAAC AAGATGTCGT TGCACAATCT GGCCATGGTG 
TTTGGACCAA CCTTGCTAAG GCCGGGCCAA ACGCAGGTGA AGCAGAAGGA TCCACTGGCA 
GCCAGCACAG TCGACGTTAT GGCTCAGGCA GGAATCCTAT ACTGCTTTCT TCAGGCACGG 
ATCAAGAAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284725.1
CDS	309..4523
Translation	MSVFDDFQRLWMQRFPQSSLSDAWEQDVRASLERHKVRINELSKELEQETLYVEYLERLLSDVERYRVSGGDPKALFEAASGSCASSNNSNCSSTTNGATQEESNTNLDKDFKSSLNLRESPSSTGAKDKDRLSLNPDNKYTTHTNAVIGGIGSEKVSGPLHSTQITADGKRTGELYLKREEDKCKDSNSEDTDGALQQCITELAASLKAQPDNVAGGRLENDVDIDKPLRKSTSQYVTVIQVKEAKDKESADGTTSSSGIGEPQQQPTPPSTFSRYSEATAPPLSPSLTSYAAHVEAKKKMPPRPPPKNIRRVEPPTIPSQQLSSSPKRETYMINTMPSSSSGSLCIDSLGNSLERNIKPSDILRQKSSDNLDSKYSAKRKTTPELSKFVNVNNPELMEELGRKMVSKADSLEQTRRNDSSPSGCGSLGRTSSTPGRSLTPGRTGVGASPTGSLSKTGKLAMADSSSTSSLERRSCLQAVDADIGTESRESGANQRSVGSKESLTSQGISEVQIKSYESVSSLSSESAKAVASQQSAAAGGALAECEPYYDSVPLDNGDGEYVFIKPGRTGSSSSRDDLSTPGSSILPLPNVADPESPGRTSNYVNIDFFLQQGIETRSSSLDSDGEYEGPPILRTISHDDQTTTQTTPGAIRKNLVSAILVCGNARGAKIRSILSSIIQSETIYVECLNKMMQYKKAIHATLTTSQPVIREEEENTIFFKIDELYDLHTGFLSDLKTIVSHEGGDVLIGEPFRRLAEMFDLYSAFLHNYKNAIETVKKCSANNPQFKKIVSTIVLNLQTEQSLTLEDLLHKPVARVQINALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQFNVVNQRLPIESNRNLRRMVRNSFIVELVDGHRKLRHLFLFNDVIACAKYKALGRDRIDYELKWFIPLKDVSIYEEADPAVELKESSPANISQVKRNLRSVRDQLAMEVANSGSGAFRSGDKYRRKLADLESQLVLATPNLVLRLGNKANNKTITFFLSSDFERTQWIDSILSLKQKCNLPGANTINSLEVTAFIVAMQKGMKTEMGSYLMRNTNDESLLVGDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPLWNESFMLELEGSQNVRILLYEAKERPLLKAKHILKLSLSWLTETTQPKSIKLTETLELGCSFRFIPGELFRGSTKPGALFGAKMSQVLKREKRDIPFIIGACIREVERRGMLEVGCYRVSGSASDLAKLKKAFESDAYEAEQLLREVDIHSVTGILKTFLRELPEALFTDQLYPRFFDTFSAFSNNNESTRINELLKVFEELPQANKASITSILDHLIRVHEKETDNKMSLHNLAMVFGPTLLRPGQTQVKQKDPLAASTVDVMAQAGILYCFLQARIKKD

Target ORF information:

RefSeq Version	NM_001297796.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297796.1

ORF Insert Sequence:

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGGTGC AGATCAAGAG CTACGAAAGC 
GTGTCTTCGC TAAGTTCGGA AAGTGCAAAG GCGGTGGCTT CCCAACAGTC GGCGGCAGCG 
GGCGGAGCTT TAGCGGAGTG TGAACCGTAC TATGATAGCG TACCGCTCGA CAACGGCGAC 
GGAGAATACG TTTTTATTAA GCCCGGCCGC ACCGGATCCT CATCCAGTCG CGATGATTTG 
TCGACGCCTG GGAGCAGCAT TTTGCCTTTG CCTAATGTAG CGGATCCCGA ATCTCCAGGC 
AGGACCTCTA ACTACGTCAA CATCGATTTC TTCCTTCAGC AAGGAATTGA GACGCGCTCC 
AGTTCGCTAG ACAGTGACGG GGAGTACGAG GGTCCGCCCA TATTACGCAC CATTTCGCAC 
GACGATCAAA CCACAACACA AACTACTCCG GGAGCAATTC GCAAGAACTT AGTTTCGGCG 
ATACTTGTTT GTGGAAATGC GCGCGGTGCT AAAATACGTT CCATACTTAG CTCAATTATT 
CAAAGTGAGA CTATCTACGT GGAATGCCTC AATAAAATGA TGCAGTACAA GAAGGCCATC 
CACGCAACCC TAACTACTTC GCAGCCTGTG ATCAGGGAGG AGGAGGAGAA CACAATTTTC 
TTTAAAATTG ATGAGCTTTA CGATCTGCAT ACCGGTTTCT TATCTGATTT AAAGACTATT 
GTGTCTCACG AGGGTGGTGA TGTGCTGATC GGTGAGCCGT TTCGTCGACT GGCGGAGATG 
TTTGACTTAT ACAGTGCCTT TTTGCACAAC TACAAAAATG CTATTGAGAC TGTGAAGAAG 
TGCAGTGCCA ATAACCCCCA GTTTAAAAAG ATCGTCTCCA CCATTGTGCT TAATCTGCAG 
ACGGAACAAT CGCTAACTCT CGAGGATCTA TTGCACAAAC CTGTTGCGAG GGTACAAATC 
AACGCATTGG TCTTCAACGA TTTGCTACGC GAAACCCCAA ATGCGCATCC GGATCACCAG 
CCATTAAGGC AGGCGCAAAA GATTATTCAG ATGTTCCTCA ATCAGTTCAA TGTGGTCAAT 
CAGCGGTTGC CCATTGAATC GAATCGAAAT TTAAGACGCA TGGTGCGTAA TTCTTTTATT 
GTAGAACTTG TCGATGGACA TCGTAAGCTG CGGCATCTCT TTCTTTTTAA TGATGTCATC 
GCATGTGCCA AATACAAAGC ATTAGGCCGC GATCGTATCG ACTATGAGCT CAAATGGTTC 
ATTCCGCTAA AAGACGTATC CATTTACGAG GAAGCAGATC CGGCGGTCGA GCTTAAGGAA 
TCTAGTCCAG CAAACATTTC GCAAGTAAAA CGCAATTTGC GATCCGTTCG AGATCAATTG 
GCCATGGAGG TGGCTAACAG TGGAAGTGGA GCCTTCCGTT CCGGTGATAA GTATCGCCGC 
AAGCTGGCGG ATCTGGAATC ACAGCTGGTG CTGGCAACGC CCAATTTAGT GTTGCGCCTG 
GGGAACAAGG CAAATAATAA AACCATAACC TTCTTTCTTA GCTCGGATTT TGAGAGGACG 
CAGTGGATCG ACTCAATCTT ATCCCTTAAG CAAAAATGCA ACCTGCCTGG AGCAAATACT 
ATTAATTCCC TGGAGGTTAC CGCCTTCATT GTAGCCATGC AGAAGGGCAT GAAAACCGAA 
ATGGGTTCAT ATCTTATGCG TAACACTAAC GACGAGAGCC TGTTGGTTGG GGACCTGTAC 
ATGGGAGTTC ATGGATTGGA AGGACTGGAG CAAGCGAACG ACCTGTACAT CTGCGTCGAG 
GTGGACTCCT ATGGCCACTA TTTTCGCAAG GCCACCACCA AGAAGATATG TCGCAGTCAA 
ACGCCGCTCT GGAACGAAAG CTTTATGCTG GAACTGGAGG GCAGCCAGAA TGTACGCATT 
CTTCTTTATG AGGCTAAGGA GCGACCGCTT CTTAAAGCCA AGCACATACT TAAGTTGAGT 
TTAAGTTGGC TGACAGAAAC AACGCAGCCG AAGTCAATTA AACTTACTGA GACCTTAGAG 
CTCGGCTGTA GCTTCCGTTT TATACCAGGC GAGCTCTTCC GCGGCAGCAC AAAGCCAGGA 
GCCCTCTTCG GTGCCAAAAT GAGTCAAGTA TTAAAACGTG AAAAACGCGA CATTCCGTTC 
ATCATAGGCG CCTGCATTCG GGAGGTAGAG CGGCGTGGGA TGCTGGAGGT TGGCTGCTAC 
CGCGTCAGCG GTTCCGCTTC TGATTTGGCG AAGCTGAAGA AGGCCTTTGA GTCTGATGCC 
TATGAGGCGG AGCAGTTGCT ACGCGAGGTG GACATCCATT CGGTCACTGG CATTCTGAAA 
ACTTTTTTAA GGGAGCTCCC CGAGGCCCTT TTCACGGATC AACTTTATCC ACGATTTTTC 
GACACATTCA GCGCCTTTAG CAACAACAAC GAATCCACAC GAATCAACGA GCTTCTAAAA 
GTTTTTGAGG AGTTGCCACA GGCCAACAAA GCCTCCATTA CTTCGATATT AGATCATTTA 
ATAAGAGTCC ACGAAAAGGA GACAGATAAC AAGATGTCGT TGCACAATCT GGCCATGGTG 
TTTGGACCAA CCTTGCTAAG GCCGGGCCAA ACGCAGGTGA AGCAGAAGGA TCCACTGGCA 
GCCAGCACAG TCGACGTTAT GGCTCAGGCA GGAATCCTAT ACTGCTTTCT TCAGGCACGG 
ATCAAGAAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP1A ( NM_001297795.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284724.1 Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant D, mRNA.

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CloneID	OFa22407
Clone ID Related Accession (Same CDS sequence)	NM_001297795.1
Accession Version	NM_001297795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 1A, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGATCA AGAGCTACGA AAGCGTGTCT 
TCGCTAAGTT CGGAAAGTGC AAAGGCGGTG GCTTCCCAAC AGTCGGCGGC AGCGGGCGGA 
GCTTTAGCGG AGTGTGAACC GTACTATGAT AGCGTACCGC TCGACAACGG CGACGGAGAA 
TACGTTTTTA TTAAGCCCGG CCGCACCGGA TCCTCATCCA GTCGCGATGA TTTGTCGACG 
CCTGGGAGCA GCATTTTGCC TTTGCCTAAT GTAGCGGATC CCGAATCTCC AGGCAGGACC 
TCTAACTACG TCAACATCGA TTTCTTCCTT CAGCAAGGAA TTGAGACGCG CTCCAGTTCG 
CTAGACAGTG ACGGGGAGTA CGAGGGTCCG CCCATATTAC GCACCATTTC GCACGACGAT 
CAAACCACAA CACAAACTAC TCCGGGAGCA ATTCGCAAGA ACTTAGTTTC GGCGATACTT 
GTTTGTGGAA ATGCGCGCGG TGCTAAAATA CGTTCCATAC TTAGCTCAAT TATTCAAAGT 
GAGACTATCT ACGTGGAATG CCTCAATAAA ATGATGCAGT ACAAGAAGGC CATCCACGCA 
ACCCTAACTA CTTCGCAGCC TGTGATCAGG GAGGAGGAGG AGAACACAAT TTTCTTTAAA 
ATTGATGAGC TTTACGATCT GCATACCGGT TTCTTATCTG ATTTAAAGAC TATTGTGTCT 
CACGAGGGTG GTGATGTGCT GATCGGTGAG CCGTTTCGTC GACTGGCGGA GATGTTTGAC 
TTATACAGTG CCTTTTTGCA CAACTACAAA AATGCTATTG AGACTGTGAA GAAGTGCAGT 
GCCAATAACC CCCAGTTTAA AAAGATCGTC TCCACCATTG TGCTTAATCT GCAGACGGAA 
CAATCGCTAA CTCTCGAGGA TCTATTGCAC AAACCTGTTG CGAGGGTACA AATCAACGCA 
TTGGTCTTCA ACGATTTGCT ACGCGAAACC CCAAATGCGC ATCCGGATCA CCAGCCATTA 
AGGCAGGCGC AAAAGATTAT TCAGATGTTC CTCAATCAGT TCAATGTGGT CAATCAGCGG 
TTGCCCATTG AATCGAATCG AAATTTAAGA CGCATGGTGC GTAATTCTTT TATTGTAGAA 
CTTGTCGATG GACATCGTAA GCTGCGGCAT CTCTTTCTTT TTAATGATGT CATCGCATGT 
GCCAAATACA AAGCATTAGG CCGCGATCGT ATCGACTATG AGCTCAAATG GTTCATTCCG 
CTAAAAGACG TATCCATTTA CGAGGAAGCA GATCCGGCGG TCGAGCTTAA GGAATCTAGT 
CCAGCAAACA TTTCGCAAGT AAAACGCAAT TTGCGATCCG TTCGAGATCA ATTGGCCATG 
GAGGTGGCTA ACAGTGGAAG TGGAGCCTTC CGTTCCGGTG ATAAGTATCG CCGCAAGCTG 
GCGGATCTGG AATCACAGCT GGTGCTGGCA ACGCCCAATT TAGTGTTGCG CCTGGGGAAC 
AAGGCAAATA ATAAAACCAT AACCTTCTTT CTTAGCTCGG ATTTTGAGAG GACGCAGTGG 
ATCGACTCAA TCTTATCCCT TAAGCAAAAA TGCAACCTGC CTGGAGCAAA TACTATTAAT 
TCCCTGGAGG TTACCGCCTT CATTGTAGCC ATGCAGAAGG GCATGAAAAC CGAAATGGGT 
TCATATCTTA TGCGTAACAC TAACGACGAG AGCCTGTTGG TTGGGGACCT GTACATGGGA 
GTTCATGGAT TGGAAGGACT GGAGCAAGCG AACGACCTGT ACATCTGCGT CGAGGTGGAC 
TCCTATGGCC ACTATTTTCG CAAGGCCACC ACCAAGAAGA TATGTCGCAG TCAAACGCCG 
CTCTGGAACG AAAGCTTTAT GCTGGAACTG GAGGGCAGCC AGAATGTACG CATTCTTCTT 
TATGAGGCTA AGGAGCGACC GCTTCTTAAA GCCAAGCACA TACTTAAGTT GAGTTTAAGT 
TGGCTGACAG AAACAACGCA GCCGAAGTCA ATTAAACTTA CTGAGACCTT AGAGCTCGGC 
TGTAGCTTCC GTTTTATACC AGGCGAGCTC TTCCGCGGCA GCACAAAGCC AGGAGCCCTC 
TTCGGTGCCA AAATGAGTCA AGTATTAAAA CGTGAAAAAC GCGACATTCC GTTCATCATA 
GGCGCCTGCA TTCGGGAGGT AGAGCGGCGT GGGATGCTGG AGGTTGGCTG CTACCGCGTC 
AGCGGTTCCG CTTCTGATTT GGCGAAGCTG AAGAAGGCCT TTGAGTCTGA TGCCTATGAG 
GCGGAGCAGT TGCTACGCGA GGTGGACATC CATTCGGTCA CTGGCATTCT GAAAACTTTT 
TTAAGGGAGC TCCCCGAGGC CCTTTTCACG GATCAACTTT ATCCACGATT TTTCGACACA 
TTCAGCGCCT TTAGCAACAA CAACGAATCC ACACGAATCA ACGAGCTTCT AAAAGTTTTT 
GAGGAGTTGC CACAGGCCAA CAAAGCCTCC ATTACTTCGA TATTAGATCA TTTAATAAGA 
GTCCACGAAA AGGAGACAGA TAACAAGATG TCGTTGCACA ATCTGGCCAT GGTGTTTGGA 
CCAACCTTGC TAAGGCCGGG CCAAACGCAG GTGAAGCAGA AGGATCCACT GGCAGCCAGC 
ACAGTCGACG TTATGGCTCA GGCAGGAATC CTATACTGCT TTCTTCAGGC ACGGATCAAG 
AAGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284724.1
CDS	309..4517
Translation	MSVFDDFQRLWMQRFPQSSLSDAWEQDVRASLERHKVRINELSKELEQETLYVEYLERLLSDVERYRVSGGDPKALFEAASGSCASSNNSNCSSTTNGATQEESNTNLDKDFKSSLNLRESPSSTGAKDKDRLSLNPDNKYTTHTNAVIGGIGSEKVSGPLHSTQITADGKRTGELYLKREEDKCKDSNSEDTDGALQQCITELAASLKAQPDNVAGGRLENDVDIDKPLRKSTSQYVTVIQVKEAKDKESADGTTSSSGIGEPQQQPTPPSTFSRYSEATAPPLSPSLTSYAAHVEAKKKMPPRPPPKNIRRVEPPTIPSQQLSSSPKRETYMINTMPSSSSGSLCIDSLGNSLERNIKPSDILRQKSSDNLDSKYSAKRKTTPELSKFVNVNNPELMEELGRKMVSKADSLEQTRRNDSSPSGCGSLGRTSSTPGRSLTPGRTGVGASPTGSLSKTGKLAMADSSSTSSLERRSCLQAVDADIGTESRESGANQRSVGSKESLTSQGISEIKSYESVSSLSSESAKAVASQQSAAAGGALAECEPYYDSVPLDNGDGEYVFIKPGRTGSSSSRDDLSTPGSSILPLPNVADPESPGRTSNYVNIDFFLQQGIETRSSSLDSDGEYEGPPILRTISHDDQTTTQTTPGAIRKNLVSAILVCGNARGAKIRSILSSIIQSETIYVECLNKMMQYKKAIHATLTTSQPVIREEEENTIFFKIDELYDLHTGFLSDLKTIVSHEGGDVLIGEPFRRLAEMFDLYSAFLHNYKNAIETVKKCSANNPQFKKIVSTIVLNLQTEQSLTLEDLLHKPVARVQINALVFNDLLRETPNAHPDHQPLRQAQKIIQMFLNQFNVVNQRLPIESNRNLRRMVRNSFIVELVDGHRKLRHLFLFNDVIACAKYKALGRDRIDYELKWFIPLKDVSIYEEADPAVELKESSPANISQVKRNLRSVRDQLAMEVANSGSGAFRSGDKYRRKLADLESQLVLATPNLVLRLGNKANNKTITFFLSSDFERTQWIDSILSLKQKCNLPGANTINSLEVTAFIVAMQKGMKTEMGSYLMRNTNDESLLVGDLYMGVHGLEGLEQANDLYICVEVDSYGHYFRKATTKKICRSQTPLWNESFMLELEGSQNVRILLYEAKERPLLKAKHILKLSLSWLTETTQPKSIKLTETLELGCSFRFIPGELFRGSTKPGALFGAKMSQVLKREKRDIPFIIGACIREVERRGMLEVGCYRVSGSASDLAKLKKAFESDAYEAEQLLREVDIHSVTGILKTFLRELPEALFTDQLYPRFFDTFSAFSNNNESTRINELLKVFEELPQANKASITSILDHLIRVHEKETDNKMSLHNLAMVFGPTLLRPGQTQVKQKDPLAASTVDVMAQAGILYCFLQARIKKD

Target ORF information:

RefSeq Version	NM_001297795.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 1A (RhoGAP1A), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297795.1

ORF Insert Sequence:

ATGAGCGTTT TCGATGACTT CCAGCGTCTG TGGATGCAGC GATTTCCGCA AAGCTCTCTA 
TCCGATGCAT GGGAACAAGA TGTTCGGGCT AGTCTCGAGC GACACAAAGT GCGCATTAAT 
GAGCTAAGCA AGGAGCTTGA GCAGGAGACG CTCTATGTGG AGTATCTAGA GCGTCTGCTG 
TCCGACGTGG AACGGTATCG CGTTTCTGGC GGGGATCCAA AGGCACTCTT TGAGGCTGCT 
AGCGGTAGTT GTGCTAGCAG CAATAACAGC AACTGCAGCA GCACCACCAA TGGAGCTACA 
CAAGAAGAAA GCAACACTAA TCTGGACAAA GATTTTAAAT CTAGTTTAAA TCTGCGCGAG 
TCTCCTTCTT CCACTGGCGC CAAGGACAAG GATCGCCTGT CGCTCAACCC AGACAACAAA 
TACACAACAC ACACAAATGC CGTTATAGGT GGGATCGGAT CGGAAAAGGT GAGCGGTCCA 
CTTCACTCCA CTCAGATCAC GGCGGACGGC AAACGAACAG GGGAGCTGTA TTTAAAGCGG 
GAAGAAGACA AATGCAAGGA TAGTAATAGT GAGGACACCG ATGGGGCCCT GCAGCAGTGC 
ATCACGGAAT TGGCGGCGTC TTTAAAAGCC CAGCCGGATA ACGTGGCAGG AGGGCGTCTA 
GAAAACGATG TCGATATCGA TAAACCGCTG CGCAAGTCGA CGTCACAATA CGTTACTGTC 
ATTCAGGTCA AAGAGGCCAA AGACAAAGAA AGCGCTGATG GAACTACCTC GTCATCGGGG 
ATCGGTGAAC CGCAGCAGCA ACCCACTCCA CCATCCACAT TTTCTCGATA TTCGGAGGCC 
ACCGCTCCAC CGCTTTCGCC ATCGCTCACA TCGTACGCTG CCCACGTAGA GGCCAAGAAG 
AAAATGCCAC CCAGACCTCC GCCAAAGAAT ATTCGGCGAG TTGAGCCCCC TACCATTCCC 
AGCCAGCAGC TATCGTCTTC GCCCAAAAGG GAGACATATA TGATCAACAC GATGCCAAGC 
AGTTCAAGCG GCAGTCTCTG CATTGACAGC CTGGGCAATT CGCTGGAGCG GAACATCAAG 
CCGTCTGATA TACTACGTCA GAAATCATCT GATAATTTGG ATTCCAAGTA CTCGGCAAAA 
CGGAAAACCA CACCGGAGCT TAGTAAATTT GTGAACGTAA ACAATCCTGA ATTAATGGAG 
GAGCTCGGTC GTAAGATGGT AAGCAAGGCA GACAGCCTGG AACAAACGAG GAGGAACGAC 
TCGTCGCCTT CTGGCTGTGG TAGCCTAGGA CGCACTTCCT CCACGCCTGG GCGATCGTTG 
ACTCCTGGAC GAACCGGAGT TGGTGCCTCG CCAACTGGTA GTCTAAGTAA AACCGGAAAA 
CTGGCAATGG CGGACAGCAG CTCCACTAGC AGTCTAGAGA GGAGATCGTG CCTGCAGGCT 
GTCGATGCGG ACATTGGAAC GGAATCGCGG GAATCGGGTG CCAACCAGCG TAGTGTAGGG 
TCCAAGGAGT CGCTGACATC ACAAGGCATA TCGGAGATCA AGAGCTACGA AAGCGTGTCT 
TCGCTAAGTT CGGAAAGTGC AAAGGCGGTG GCTTCCCAAC AGTCGGCGGC AGCGGGCGGA 
GCTTTAGCGG AGTGTGAACC GTACTATGAT AGCGTACCGC TCGACAACGG CGACGGAGAA 
TACGTTTTTA TTAAGCCCGG CCGCACCGGA TCCTCATCCA GTCGCGATGA TTTGTCGACG 
CCTGGGAGCA GCATTTTGCC TTTGCCTAAT GTAGCGGATC CCGAATCTCC AGGCAGGACC 
TCTAACTACG TCAACATCGA TTTCTTCCTT CAGCAAGGAA TTGAGACGCG CTCCAGTTCG 
CTAGACAGTG ACGGGGAGTA CGAGGGTCCG CCCATATTAC GCACCATTTC GCACGACGAT 
CAAACCACAA CACAAACTAC TCCGGGAGCA ATTCGCAAGA ACTTAGTTTC GGCGATACTT 
GTTTGTGGAA ATGCGCGCGG TGCTAAAATA CGTTCCATAC TTAGCTCAAT TATTCAAAGT 
GAGACTATCT ACGTGGAATG CCTCAATAAA ATGATGCAGT ACAAGAAGGC CATCCACGCA 
ACCCTAACTA CTTCGCAGCC TGTGATCAGG GAGGAGGAGG AGAACACAAT TTTCTTTAAA 
ATTGATGAGC TTTACGATCT GCATACCGGT TTCTTATCTG ATTTAAAGAC TATTGTGTCT 
CACGAGGGTG GTGATGTGCT GATCGGTGAG CCGTTTCGTC GACTGGCGGA GATGTTTGAC 
TTATACAGTG CCTTTTTGCA CAACTACAAA AATGCTATTG AGACTGTGAA GAAGTGCAGT 
GCCAATAACC CCCAGTTTAA AAAGATCGTC TCCACCATTG TGCTTAATCT GCAGACGGAA 
CAATCGCTAA CTCTCGAGGA TCTATTGCAC AAACCTGTTG CGAGGGTACA AATCAACGCA 
TTGGTCTTCA ACGATTTGCT ACGCGAAACC CCAAATGCGC ATCCGGATCA CCAGCCATTA 
AGGCAGGCGC AAAAGATTAT TCAGATGTTC CTCAATCAGT TCAATGTGGT CAATCAGCGG 
TTGCCCATTG AATCGAATCG AAATTTAAGA CGCATGGTGC GTAATTCTTT TATTGTAGAA 
CTTGTCGATG GACATCGTAA GCTGCGGCAT CTCTTTCTTT TTAATGATGT CATCGCATGT 
GCCAAATACA AAGCATTAGG CCGCGATCGT ATCGACTATG AGCTCAAATG GTTCATTCCG 
CTAAAAGACG TATCCATTTA CGAGGAAGCA GATCCGGCGG TCGAGCTTAA GGAATCTAGT 
CCAGCAAACA TTTCGCAAGT AAAACGCAAT TTGCGATCCG TTCGAGATCA ATTGGCCATG 
GAGGTGGCTA ACAGTGGAAG TGGAGCCTTC CGTTCCGGTG ATAAGTATCG CCGCAAGCTG 
GCGGATCTGG AATCACAGCT GGTGCTGGCA ACGCCCAATT TAGTGTTGCG CCTGGGGAAC 
AAGGCAAATA ATAAAACCAT AACCTTCTTT CTTAGCTCGG ATTTTGAGAG GACGCAGTGG 
ATCGACTCAA TCTTATCCCT TAAGCAAAAA TGCAACCTGC CTGGAGCAAA TACTATTAAT 
TCCCTGGAGG TTACCGCCTT CATTGTAGCC ATGCAGAAGG GCATGAAAAC CGAAATGGGT 
TCATATCTTA TGCGTAACAC TAACGACGAG AGCCTGTTGG TTGGGGACCT GTACATGGGA 
GTTCATGGAT TGGAAGGACT GGAGCAAGCG AACGACCTGT ACATCTGCGT CGAGGTGGAC 
TCCTATGGCC ACTATTTTCG CAAGGCCACC ACCAAGAAGA TATGTCGCAG TCAAACGCCG 
CTCTGGAACG AAAGCTTTAT GCTGGAACTG GAGGGCAGCC AGAATGTACG CATTCTTCTT 
TATGAGGCTA AGGAGCGACC GCTTCTTAAA GCCAAGCACA TACTTAAGTT GAGTTTAAGT 
TGGCTGACAG AAACAACGCA GCCGAAGTCA ATTAAACTTA CTGAGACCTT AGAGCTCGGC 
TGTAGCTTCC GTTTTATACC AGGCGAGCTC TTCCGCGGCA GCACAAAGCC AGGAGCCCTC 
TTCGGTGCCA AAATGAGTCA AGTATTAAAA CGTGAAAAAC GCGACATTCC GTTCATCATA 
GGCGCCTGCA TTCGGGAGGT AGAGCGGCGT GGGATGCTGG AGGTTGGCTG CTACCGCGTC 
AGCGGTTCCG CTTCTGATTT GGCGAAGCTG AAGAAGGCCT TTGAGTCTGA TGCCTATGAG 
GCGGAGCAGT TGCTACGCGA GGTGGACATC CATTCGGTCA CTGGCATTCT GAAAACTTTT 
TTAAGGGAGC TCCCCGAGGC CCTTTTCACG GATCAACTTT ATCCACGATT TTTCGACACA 
TTCAGCGCCT TTAGCAACAA CAACGAATCC ACACGAATCA ACGAGCTTCT AAAAGTTTTT 
GAGGAGTTGC CACAGGCCAA CAAAGCCTCC ATTACTTCGA TATTAGATCA TTTAATAAGA 
GTCCACGAAA AGGAGACAGA TAACAAGATG TCGTTGCACA ATCTGGCCAT GGTGTTTGGA 
CCAACCTTGC TAAGGCCGGG CCAAACGCAG GTGAAGCAGA AGGATCCACT GGCAGCCAGC 
ACAGTCGACG TTATGGCTCA GGCAGGAATC CTATACTGCT TTCTTCAGGC ACGGATCAAG 
AAGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAP1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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