ddbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddbt
Entrez Gene ID	5740226
Full Name	CG34264 gene product from transcript CG34264-RC
Synonyms	Dmel\CG34264,HDC07730
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C1-61C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144393.2	NP_001137865.1	deadbeat, isoform C
NM_001144392.1	NP_001137864.1	deadbeat, isoform B
NM_001103990.3	NP_001097460.1	deadbeat, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddbt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddbt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19690	NM_001144392.1 Latest version!	Drosophila melanogaster deadbeat (ddbt), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa19690	NM_001144393.2 Latest version!	Drosophila melanogaster deadbeat (ddbt), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa19691	NM_001103990.3 Latest version!	Drosophila melanogaster deadbeat (ddbt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddbt ( NM_001144393.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137865.1 Drosophila melanogaster deadbeat (ddbt), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19690
Clone ID Related Accession (Same CDS sequence)	NM_001144393.2 , NM_001144392.1
Accession Version	NM_001144393.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deadbeat, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001144393.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCGTT CCCAGTCGAA AATGATTCGG GCCGCCATGG TCGTGGACGA CCCTAATATG 
GTGGCCTGGC TGCCATACCT TAACTTTCTT CGCTTTCTGA AGCGAAACTT CTATCCCAGG 
ACTGATTTGC GCCGCTTACT TCAGGTGGGA CTCATCCGTT GGATCGCACT TAGCGATGCC 
CAGAAGAGGT TATTTGAGCC AGAACGAATC CTGGCGCGTG TGGCTCGCAG GCAAAGGAAT 
AAACGACGCC GCCGACTACT TCGCCGTGCT CGGCATGGCC AGAAAGGACG TGGTGCAGTG 
CGACGCCCCA TCTACGACAG CCGTCCTAAA CCGCGACGCC GCAAACCGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137865.1
CDS	125..478
Translation	MARSQSKMIRAAMVVDDPNMVAWLPYLNFLRFLKRNFYPRTDLRRLLQVGLIRWIALSDAQKRLFEPERILARVARRQRNKRRRRLLRRARHGQKGRGAVRRPIYDSRPKPRRRKPK

Target ORF information:

RefSeq Version	NM_001144393.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deadbeat (ddbt), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144393.2

ORF Insert Sequence:

ATGGCGCGTT CCCAGTCGAA AATGATTCGG GCCGCCATGG TCGTGGACGA CCCTAATATG 
GTGGCCTGGC TGCCATACCT TAACTTTCTT CGCTTTCTGA AGCGAAACTT CTATCCCAGG 
ACTGATTTGC GCCGCTTACT TCAGGTGGGA CTCATCCGTT GGATCGCACT TAGCGATGCC 
CAGAAGAGGT TATTTGAGCC AGAACGAATC CTGGCGCGTG TGGCTCGCAG GCAAAGGAAT 
AAACGACGCC GCCGACTACT TCGCCGTGCT CGGCATGGCC AGAAAGGACG TGGTGCAGTG 
CGACGCCCCA TCTACGACAG CCGTCCTAAA CCGCGACGCC GCAAACCGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddbt ( NM_001144392.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137864.1 Drosophila melanogaster deadbeat (ddbt), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19690
Clone ID Related Accession (Same CDS sequence)	NM_001144393.2 , NM_001144392.1
Accession Version	NM_001144392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deadbeat, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCGTT CCCAGTCGAA AATGATTCGG GCCGCCATGG TCGTGGACGA CCCTAATATG 
GTGGCCTGGC TGCCATACCT TAACTTTCTT CGCTTTCTGA AGCGAAACTT CTATCCCAGG 
ACTGATTTGC GCCGCTTACT TCAGGTGGGA CTCATCCGTT GGATCGCACT TAGCGATGCC 
CAGAAGAGGT TATTTGAGCC AGAACGAATC CTGGCGCGTG TGGCTCGCAG GCAAAGGAAT 
AAACGACGCC GCCGACTACT TCGCCGTGCT CGGCATGGCC AGAAAGGACG TGGTGCAGTG 
CGACGCCCCA TCTACGACAG CCGTCCTAAA CCGCGACGCC GCAAACCGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137864.1
CDS	122..475
Translation	MARSQSKMIRAAMVVDDPNMVAWLPYLNFLRFLKRNFYPRTDLRRLLQVGLIRWIALSDAQKRLFEPERILARVARRQRNKRRRRLLRRARHGQKGRGAVRRPIYDSRPKPRRRKPK

Target ORF information:

RefSeq Version	NM_001144392.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deadbeat (ddbt), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144392.1

ORF Insert Sequence:

ATGGCGCGTT CCCAGTCGAA AATGATTCGG GCCGCCATGG TCGTGGACGA CCCTAATATG 
GTGGCCTGGC TGCCATACCT TAACTTTCTT CGCTTTCTGA AGCGAAACTT CTATCCCAGG 
ACTGATTTGC GCCGCTTACT TCAGGTGGGA CTCATCCGTT GGATCGCACT TAGCGATGCC 
CAGAAGAGGT TATTTGAGCC AGAACGAATC CTGGCGCGTG TGGCTCGCAG GCAAAGGAAT 
AAACGACGCC GCCGACTACT TCGCCGTGCT CGGCATGGCC AGAAAGGACG TGGTGCAGTG 
CGACGCCCCA TCTACGACAG CCGTCCTAAA CCGCGACGCC GCAAACCGAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddbt ( NM_001103990.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097460.1 Drosophila melanogaster deadbeat (ddbt), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa19691
Clone ID Related Accession (Same CDS sequence)	NM_001103990.3
Accession Version	NM_001103990.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deadbeat, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001103990.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCGTT CCCAGTCGAA AATGATTCGG GCCGCCATGG TCGTGGACGA CCCTAATATG 
GTGGCCTGGC TGCCATACCT TAACTTTCTT CGCTTTCTGA AGCGAAACTT CTATCCCAGG 
ACTGATTTGC GCCGCTTACT TCAGGTGGGA CTCATCCGTT GGATCGCACT TAGCGATGCC 
CAGAAGAGCG AATCCTGGCG CGTGTGGCTC GCAGGCAAAG GAATAAACGA CGCCGCCGAC 
TACTTCGCCG TGCTCGGCAT GGCCAGAAAG GACGTGGTGC AGTGCGACGC CCCATCTACG 
ACAGCCGTCC TAAACCGCGA CGCCGCAAAC CGAAGTAGAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097460.1
CDS	125..469
Translation	MARSQSKMIRAAMVVDDPNMVAWLPYLNFLRFLKRNFYPRTDLRRLLQVGLIRWIALSDAQKSESWRVWLAGKGINDAADYFAVLGMARKDVVQCDAPSTTAVLNRDAANRSRE

Target ORF information:

RefSeq Version	NM_001103990.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deadbeat (ddbt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103990.3

ORF Insert Sequence:

ATGGCGCGTT CCCAGTCGAA AATGATTCGG GCCGCCATGG TCGTGGACGA CCCTAATATG 
GTGGCCTGGC TGCCATACCT TAACTTTCTT CGCTTTCTGA AGCGAAACTT CTATCCCAGG 
ACTGATTTGC GCCGCTTACT TCAGGTGGGA CTCATCCGTT GGATCGCACT TAGCGATGCC 
CAGAAGAGCG AATCCTGGCG CGTGTGGCTC GCAGGCAAAG GAATAAACGA CGCCGCCGAC 
TACTTCGCCG TGCTCGGCAT GGCCAGAAAG GACGTGGTGC AGTGCGACGC CCCATCTACG 
ACAGCCGTCC TAAACCGCGA CGCCGCAAAC CGAAGTAGAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ddbt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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