Jafrac1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Jafrac1
Entrez Gene ID	53578
Full Name	thioredoxin peroxidase 1
Synonyms	1633,CG1633,DPx-4783,DPx4783,DmTPx-1,Dmel\CG1633,Dpx-4783,JAFRAC 1,Jafrac,PRDX1_DROME,Prx,Prx4783,SSP5111,T20,cTPx,dPrx I,jafrac1,jf1
General protein information	Preferred Names thioredoxin peroxidase 1 Names CG1633 gene product from transcript CG1633-RD CG1633-PA CG1633-PB CG1633-PC CG1633-PD CG1633-PE Jafrac1-PA Jafrac1-PB Jafrac1-PC Jafrac1-PD Jafrac1-PE peroxidase peroxiredoxin peroxiredoxin 1 peroxiredoxin 4783 peroxiredoxin I thioredoxin peroxidase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11E11-11E11

mRNA and Protein(s)

mRNA	Protein	Name
NM_167359.2	NP_727689.1	thioredoxin peroxidase 1, isoform B
NM_058162.3	NP_477510.1	thioredoxin peroxidase 1, isoform A
NM_001298274.1	NP_001285203.1	thioredoxin peroxidase 1, isoform D
NM_001298275.1	NP_001285204.1	thioredoxin peroxidase 1, isoform E
NM_001298273.1	NP_001285202.1	thioredoxin peroxidase 1, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prdx2	NP_035693.3
Anopheles gambiae (African malaria mosquito)	TPX2	XP_308081.2
Arabidopsis thaliana (thale cress)	AT3G11630	NP_187769.1
Arabidopsis thaliana (thale cress)	2-Cys Prx B	NP_568166.1
Saccharomyces cerevisiae (baker's yeast)	TSA1	NP_013684.1
Pan troglodytes (chimpanzee)	PRDX2	XP_003953433.1
Bos taurus (cattle)	PRDX2	NP_777188.1
Schizosaccharomyces pombe (fission yeast)	tpx1	NP_588430.1
Homo sapiens (human)	PRDX2	NP_005800.3
Canis lupus familiaris (dog)	PRDX2	XP_003639771.1
Rattus norvegicus (Norway rat)	Prdx2	NP_058865.1
Danio rerio (zebrafish)	prdx2	NP_001002468.1
Drosophila melanogaster (fruit fly)	Jafrac1	NP_477510.1
Caenorhabditis elegans (roundworm)	prdx-2	NP_001122604.1
Oryza sativa (rice)	Os02g0537700	NP_001047050.1
Xenopus tropicalis (tropical clawed frog)	prdx2	NP_989001.1
	PRDX2	XP_001109216.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Jafrac1-mediated reduction of hydrogen peroxide is required to maintain DE-cadherin protein levels in the early embryo.
Title: Peroxiredoxin stabilization of DE-cadherin promotes primordial germ cell adhesion.

Activation of JNK/FOXO signaling in neurons increased the Jafrac1 expression level under both normal and oxidative stressed conditions.
Title: JNK/FOXO-mediated neuronal expression of fly homologue of peroxiredoxin II reduces oxidative stress and extends life span.

gene for thioredoxin peroxidase (DmTPx-1) was cloned and expressed, and the protein characterized
Title: Thioredoxin-2 but not thioredoxin-1 is a substrate of thioredoxin peroxidase-1 from Drosophila melanogaster: isolation and characterization of a second thioredoxin in D. Melanogaster and evidence for distinct biological functions of Trx-1 and Trx-2.

The following Jafrac1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Jafrac1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02466	NM_001298273.1 Latest version!	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02466	NM_058162.3 Latest version!	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02466	NM_001298275.1 Latest version!	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02466	NM_001298274.1 Latest version!	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa02466	NM_167359.2 Latest version!	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Jafrac1 ( NM_167359.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727689.1 Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02466
Clone ID Related Accession (Same CDS sequence)	NM_167359.2 , NM_058162.3 , NM_001298274.1 , NM_001298275.1 , NM_001298273.1
Accession Version	NM_167359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin peroxidase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167359.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727689.1
CDS	142..726
Translation	MPQLQKPAPAFAGTAVVNGVFKDIKLSDYKGKYLVLFFYPLDFTFVCPTEIIAFSESAAEFRKINCEVIGCSTDSQFTHLAWINTPRKQGGLGSMDIPLLADKSMKVARDYGVLDEETGIPFRGLFIIDDKQNLRQITVNDLPVGRSVEETLRLVQAFQYTDKYGEVCPANWKPGQKTMVADPTKSKEYFETTS

Target ORF information:

RefSeq Version	NM_167359.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167359.2

ORF Insert Sequence:

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Jafrac1 ( NM_058162.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477510.1 Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02466
Clone ID Related Accession (Same CDS sequence)	NM_167359.2 , NM_058162.3 , NM_001298274.1 , NM_001298275.1 , NM_001298273.1
Accession Version	NM_058162.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin peroxidase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_058162.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477510.1
CDS	138..722
Translation	MPQLQKPAPAFAGTAVVNGVFKDIKLSDYKGKYLVLFFYPLDFTFVCPTEIIAFSESAAEFRKINCEVIGCSTDSQFTHLAWINTPRKQGGLGSMDIPLLADKSMKVARDYGVLDEETGIPFRGLFIIDDKQNLRQITVNDLPVGRSVEETLRLVQAFQYTDKYGEVCPANWKPGQKTMVADPTKSKEYFETTS

Target ORF information:

RefSeq Version	NM_058162.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058162.3

ORF Insert Sequence:

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Jafrac1 ( NM_001298274.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285203.1 Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa02466
Clone ID Related Accession (Same CDS sequence)	NM_167359.2 , NM_058162.3 , NM_001298274.1 , NM_001298275.1 , NM_001298273.1
Accession Version	NM_001298274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin peroxidase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285203.1
CDS	208..792
Translation	MPQLQKPAPAFAGTAVVNGVFKDIKLSDYKGKYLVLFFYPLDFTFVCPTEIIAFSESAAEFRKINCEVIGCSTDSQFTHLAWINTPRKQGGLGSMDIPLLADKSMKVARDYGVLDEETGIPFRGLFIIDDKQNLRQITVNDLPVGRSVEETLRLVQAFQYTDKYGEVCPANWKPGQKTMVADPTKSKEYFETTS

Target ORF information:

RefSeq Version	NM_001298274.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298274.1

ORF Insert Sequence:

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Jafrac1 ( NM_001298275.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285204.1 Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa02466
Clone ID Related Accession (Same CDS sequence)	NM_167359.2 , NM_058162.3 , NM_001298274.1 , NM_001298275.1 , NM_001298273.1
Accession Version	NM_001298275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin peroxidase 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285204.1
CDS	104..688
Translation	MPQLQKPAPAFAGTAVVNGVFKDIKLSDYKGKYLVLFFYPLDFTFVCPTEIIAFSESAAEFRKINCEVIGCSTDSQFTHLAWINTPRKQGGLGSMDIPLLADKSMKVARDYGVLDEETGIPFRGLFIIDDKQNLRQITVNDLPVGRSVEETLRLVQAFQYTDKYGEVCPANWKPGQKTMVADPTKSKEYFETTS

Target ORF information:

RefSeq Version	NM_001298275.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298275.1

ORF Insert Sequence:

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Jafrac1 ( NM_001298273.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285202.1 Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02466
Clone ID Related Accession (Same CDS sequence)	NM_167359.2 , NM_058162.3 , NM_001298274.1 , NM_001298275.1 , NM_001298273.1
Accession Version	NM_001298273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thioredoxin peroxidase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285202.1
CDS	179..763
Translation	MPQLQKPAPAFAGTAVVNGVFKDIKLSDYKGKYLVLFFYPLDFTFVCPTEIIAFSESAAEFRKINCEVIGCSTDSQFTHLAWINTPRKQGGLGSMDIPLLADKSMKVARDYGVLDEETGIPFRGLFIIDDKQNLRQITVNDLPVGRSVEETLRLVQAFQYTDKYGEVCPANWKPGQKTMVADPTKSKEYFETTS

Target ORF information:

RefSeq Version	NM_001298273.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thioredoxin peroxidase 1 (Jafrac1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298273.1

ORF Insert Sequence:

ATGCCCCAGC TACAGAAGCC CGCTCCCGCA TTCGCCGGCA CCGCCGTCGT CAACGGTGTT 
TTCAAGGACA TCAAGTTGAG CGACTACAAG GGCAAATACC TGGTGCTTTT CTTCTACCCG 
CTGGACTTCA CCTTCGTGTG CCCCACCGAG ATCATTGCGT TCTCGGAGAG CGCCGCCGAG 
TTCCGCAAGA TCAATTGCGA GGTGATCGGT TGCTCCACGG ACAGCCAGTT CACCCACTTG 
GCCTGGATCA ACACGCCAAG GAAGCAGGGT GGTCTGGGCA GCATGGACAT TCCACTGCTG 
GCTGACAAGT CGATGAAGGT GGCCCGCGAC TATGGAGTGC TCGATGAGGA GACTGGCATC 
CCCTTCCGCG GCCTGTTCAT CATCGATGAC AAGCAGAACT TGCGCCAGAT CACCGTCAAC 
GATCTGCCCG TAGGTCGCAG TGTGGAGGAG ACCCTGCGTC TCGTCCAGGC CTTCCAGTAC 
ACCGACAAGT ACGGCGAGGT CTGCCCCGCC AACTGGAAGC CCGGCCAGAA GACCATGGTG 
GCCGATCCCA CCAAGTCCAA GGAGTACTTC GAGACCACCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Jafrac1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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