pins cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pins
Entrez Gene ID	53569
Full Name	partner of inscuteable
Synonyms	CG5692,Dmel\CG5692,PINS,Pins,Rad,Raps,rad,raps,rapsyn
General protein information	Preferred Names partner of inscuteable Names CG5692 gene product from transcript CG5692-RA CG5692-PA partner of inscutable pins-PA rapsinoid rapsynoid
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98A8-98A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_080260.4	NP_524999.2	partner of inscuteable

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPSM2	NP_037428.3
	GPSM2	XP_002801724.1
Canis lupus familiaris (dog)	GPSM2	XP_547248.3
Drosophila melanogaster (fruit fly)	raps	NP_524999.2
Mus musculus (house mouse)	Gpsm2	NP_083798.2
Gallus gallus (chicken)	GPSM2	XP_004943149.1
Danio rerio (zebrafish)	gpsm2	NP_956732.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008977	XP_319728.4
Pan troglodytes (chimpanzee)	GPSM2	XP_001146355.2
Rattus norvegicus (Norway rat)	Gpsm2	NP_001178891.1
Caenorhabditis elegans (roundworm)	ags-3	NP_741788.1
Xenopus tropicalis (tropical clawed frog)	gpsm2	XP_002932195.2
Bos taurus (cattle)	GPSM2	NP_001179284.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pins is not required in the wing disc because there are parallel mechanisms for Mud localization and hence spindle orientation, making it a more robust system than in other epithelia.
Title: Pins is not required for spindle orientation in the Drosophila wing disc.

Prefoldin acts synergistically with Pins to regulate asymmetric division of both neuroblasts and Intermediate Neural Progenitors.
Title: Prefoldin and Pins synergistically regulate asymmetric division and suppress dedifferentiation.

The sterical hindrance requiring such a rotation probably contributes to the guanine nucleotide exchange inhibitor activity of GoLoco1 and Pins as a whole.
Title: Mode of interaction of the G?o subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine nucleotides.

Insc-Pins complex requires Galphai for partial activity and that the complex specifically recruits Dlg but not Mud.
Title: Inscuteable regulates the Pins-Mud spindle orientation pathway.

The Canoe-Pins(TPR) interaction recruits Canoe to the cell cortex and is required for activation of the Pins(TPR)-Mud (nuclear mitotic apparatus in mammals) spindle orientation pathway
Title: Canoe binds RanGTP to promote Pins(TPR)/Mud-mediated spindle orientation.

The following pins gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pins cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08763	NM_080260.4 Latest version!	Drosophila melanogaster partner of inscuteable (pins), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pins ( NM_080260.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524999.2 Drosophila melanogaster partner of inscuteable (pins), mRNA.

Price & Availability↑

CloneID	OFa08763
Clone ID Related Accession (Same CDS sequence)	NM_080260.4
Accession Version	NM_080260.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of inscuteable
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_080260.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTCGC TCTCTGCGTC CGCGGAGAAT GTGTCCAGTT TGGGACTGGG ATCGGGCGGA 
GGAGGAACTA CTTCCCACGA CGGCAACTCC CAGCAGGGTT CCGGATCCGA CGGAGGATCC 
AGTATGTGCC TGGAACTGGC CCTCGAGGGT GAACGCCTCT GCAAGGCGGG TGACTGCCGG 
GCGGGCGTGG CCTTCTTCCA GGCGGCCATT CAGGCCGGAA CCGAGGATCT TCGCACTCTG 
TCCGCCATCT ACTCCCAGCT GGGCAACGCC TACTTCTACC TAGGCGACTA CAACAAGGCG 
ATGCAGTACC ACAAACACGA TCTTACGCTG GCCAAGAGCA TGAACGACCG ACTGGGCGAG 
GCCAAGTCCT CTGGCAATCT GGGCAACACC CTCAAGGTAA TGGGTCGCTT CGACGAGGCG 
GCCATCTGTT GCGAGAGGCA CCTAACGCTG GCCAGGCAGC TGGGAGATCG TCTGTCCGAG 
GGACGCGCTC TTTACAACCT AGGAAATGTC TACCACGCCA AGGGCAAGCA CTTGGGTCAG 
AGAAACCCTG GAAAGTTCGG CGACGATGTC AAGGAGGCGC TCACCCGTGC CGTGGAGTTT 
TACCAGGAGA ATCTGAAGCT CATGCGGGAT CTGGGTGATC GTGGTGCTCA AGGTCGAGCT 
TGTGGTAATC TGGGAAATAC CTACTACCTA CTAGGCGATT TCCAAGCGGC CATTGAGCAT 
CATCAGGAGC GACTGCGCAT TGCCAGAGAG TTTGGTGATC GCGCGGCTGA GCGAAGGGCC 
AACAGTAATC TTGGCAACTC GCACATATTC CTGGGACAAT TCGAGGACGC CGCCGAACAC 
TACAAACGCA CCTTGGCTTT GGCCGTTGAG TTGGGTGAAC GCGAAGTGGA GGCCCAGAGT 
TGCTACAGCT TGGGCAACAC CTACACGTTG CTGCACGAGT TCAACACTGC CATCGAGTAT 
CACAATCGAC ACTTGGCCAT TGCCCAGGAG TTGGGCGATC GAATTGGTGA GGCCAGGGCC 
TGCTGGTCAC TGGGCAATGC CCACTCTGCC ATCGGTGGTC ATGAGCGGGC CCTAAAGTAT 
GCGGAACAGC ATCTTCAACT GGCCAAGGAG CTGCACGACC CGGTGGGTGA AAGCACAGCC 
AGGGTGAACA TCTCCGATCT ACGAAAGCTA CTCGGAATGC CCGACTCCGA GCCTTCACCC 
ACCGAGGAGG AGGCTCGATC CACCGCCTCC GATCACTCGG CCAGCGGAAA TCAGTCGGAT 
GGATCGGAGA ACTCGCAAGG AAGAATGGTG CGCGTTCGTC GCCAGAGCAT GGAGCAGTTG 
GACCTGATCA AGATCACTCC CGATGGAAAG CGCATGCAGG AGGAAAAGCT ACGAGCACAG 
GCGACCAGGA AAGCCAAAGA CGACGACTTT TTCGAGATGC TTTCGCGGTC GCAGTCGAAG 
CGCATGGATG ACCAGCGCTG TTCGATCAAG GTGAATCCCG CCGGAGCTCC GGCGGTGGCC 
ACTGGCGCCA CGCGTAAGCC GCTTGTGCAG CAGAACTCGT TGTTCGTGGA TCCCACCAAC 
CTGCCTGGCC TCAAGTCACC GTCGTCGGCG AATCCATCAG CCATTGGACA TGGGCCGCTC 
GCCAGGAGCG CCACCACTAC GCAGCAGCCC GATGATGACT TCCTAGACAT GCTTATGCGC 
TGCCAAGGCT CGCGTCTGGA GGAGCAGCGG TCCGAGTTAC CGCGCCCCAA TGTCACCATG 
GACGCAGAGG CGGAGGCACC ACCGCGATCC GTCCCAGAGG CTGCTGTTCC AGGGGCACCA 
CGCGGACAGA CCGGACGTGG AGCTACCGTC CCCGATGAGG ACTTCTTCTC ACTGATCATG 
AAGGTCCAGA GCGGTCGCAT GGAGGATCAG CGCGCCTCGA TTCCGTTCCG AAATGCCAAC 
AATAACAACA ATAGCCGCAG CAACAACAAC GGTTCAGCCG GCGGAGCTGG AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524999.2
CDS	249..2225
Translation	MSSLSASAENVSSLGLGSGGGGTTSHDGNSQQGSGSDGGSSMCLELALEGERLCKAGDCRAGVAFFQAAIQAGTEDLRTLSAIYSQLGNAYFYLGDYNKAMQYHKHDLTLAKSMNDRLGEAKSSGNLGNTLKVMGRFDEAAICCERHLTLARQLGDRLSEGRALYNLGNVYHAKGKHLGQRNPGKFGDDVKEALTRAVEFYQENLKLMRDLGDRGAQGRACGNLGNTYYLLGDFQAAIEHHQERLRIAREFGDRAAERRANSNLGNSHIFLGQFEDAAEHYKRTLALAVELGEREVEAQSCYSLGNTYTLLHEFNTAIEYHNRHLAIAQELGDRIGEARACWSLGNAHSAIGGHERALKYAEQHLQLAKELHDPVGESTARVNISDLRKLLGMPDSEPSPTEEEARSTASDHSASGNQSDGSENSQGRMVRVRRQSMEQLDLIKITPDGKRMQEEKLRAQATRKAKDDDFFEMLSRSQSKRMDDQRCSIKVNPAGAPAVATGATRKPLVQQNSLFVDPTNLPGLKSPSSANPSAIGHGPLARSATTTQQPDDDFLDMLMRCQGSRLEEQRSELPRPNVTMDAEAEAPPRSVPEAAVPGAPRGQTGRGATVPDEDFFSLIMKVQSGRMEDQRASIPFRNANNNNNSRSNNNGSAGGAGK

Target ORF information:

RefSeq Version	NM_080260.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of inscuteable (pins), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080260.4

ORF Insert Sequence:

ATGTCCTCGC TCTCTGCGTC CGCGGAGAAT GTGTCCAGTT TGGGACTGGG ATCGGGCGGA 
GGAGGAACTA CTTCCCACGA CGGCAACTCC CAGCAGGGTT CCGGATCCGA CGGAGGATCC 
AGTATGTGCC TGGAACTGGC CCTCGAGGGT GAACGCCTCT GCAAGGCGGG TGACTGCCGG 
GCGGGCGTGG CCTTCTTCCA GGCGGCCATT CAGGCCGGAA CCGAGGATCT TCGCACTCTG 
TCCGCCATCT ACTCCCAGCT GGGCAACGCC TACTTCTACC TAGGCGACTA CAACAAGGCG 
ATGCAGTACC ACAAACACGA TCTTACGCTG GCCAAGAGCA TGAACGACCG ACTGGGCGAG 
GCCAAGTCCT CTGGCAATCT GGGCAACACC CTCAAGGTAA TGGGTCGCTT CGACGAGGCG 
GCCATCTGTT GCGAGAGGCA CCTAACGCTG GCCAGGCAGC TGGGAGATCG TCTGTCCGAG 
GGACGCGCTC TTTACAACCT AGGAAATGTC TACCACGCCA AGGGCAAGCA CTTGGGTCAG 
AGAAACCCTG GAAAGTTCGG CGACGATGTC AAGGAGGCGC TCACCCGTGC CGTGGAGTTT 
TACCAGGAGA ATCTGAAGCT CATGCGGGAT CTGGGTGATC GTGGTGCTCA AGGTCGAGCT 
TGTGGTAATC TGGGAAATAC CTACTACCTA CTAGGCGATT TCCAAGCGGC CATTGAGCAT 
CATCAGGAGC GACTGCGCAT TGCCAGAGAG TTTGGTGATC GCGCGGCTGA GCGAAGGGCC 
AACAGTAATC TTGGCAACTC GCACATATTC CTGGGACAAT TCGAGGACGC CGCCGAACAC 
TACAAACGCA CCTTGGCTTT GGCCGTTGAG TTGGGTGAAC GCGAAGTGGA GGCCCAGAGT 
TGCTACAGCT TGGGCAACAC CTACACGTTG CTGCACGAGT TCAACACTGC CATCGAGTAT 
CACAATCGAC ACTTGGCCAT TGCCCAGGAG TTGGGCGATC GAATTGGTGA GGCCAGGGCC 
TGCTGGTCAC TGGGCAATGC CCACTCTGCC ATCGGTGGTC ATGAGCGGGC CCTAAAGTAT 
GCGGAACAGC ATCTTCAACT GGCCAAGGAG CTGCACGACC CGGTGGGTGA AAGCACAGCC 
AGGGTGAACA TCTCCGATCT ACGAAAGCTA CTCGGAATGC CCGACTCCGA GCCTTCACCC 
ACCGAGGAGG AGGCTCGATC CACCGCCTCC GATCACTCGG CCAGCGGAAA TCAGTCGGAT 
GGATCGGAGA ACTCGCAAGG AAGAATGGTG CGCGTTCGTC GCCAGAGCAT GGAGCAGTTG 
GACCTGATCA AGATCACTCC CGATGGAAAG CGCATGCAGG AGGAAAAGCT ACGAGCACAG 
GCGACCAGGA AAGCCAAAGA CGACGACTTT TTCGAGATGC TTTCGCGGTC GCAGTCGAAG 
CGCATGGATG ACCAGCGCTG TTCGATCAAG GTGAATCCCG CCGGAGCTCC GGCGGTGGCC 
ACTGGCGCCA CGCGTAAGCC GCTTGTGCAG CAGAACTCGT TGTTCGTGGA TCCCACCAAC 
CTGCCTGGCC TCAAGTCACC GTCGTCGGCG AATCCATCAG CCATTGGACA TGGGCCGCTC 
GCCAGGAGCG CCACCACTAC GCAGCAGCCC GATGATGACT TCCTAGACAT GCTTATGCGC 
TGCCAAGGCT CGCGTCTGGA GGAGCAGCGG TCCGAGTTAC CGCGCCCCAA TGTCACCATG 
GACGCAGAGG CGGAGGCACC ACCGCGATCC GTCCCAGAGG CTGCTGTTCC AGGGGCACCA 
CGCGGACAGA CCGGACGTGG AGCTACCGTC CCCGATGAGG ACTTCTTCTC ACTGATCATG 
AAGGTCCAGA GCGGTCGCAT GGAGGATCAG CGCGCCTCGA TTCCGTTCCG AAATGCCAAC 
AATAACAACA ATAGCCGCAG CAACAACAAC GGTTCAGCCG GCGGAGCTGG AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pins cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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