esn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	esn
Entrez Gene ID	53557
Full Name	espinas
Synonyms	CG12833,CG12835,CG43722,Dm_2R:11728,Dmel\CG43722,Dmel_CG12833,Dmel_CG12835
General protein information	Preferred Names espinas Names CG43722 gene product from transcript CG43722-RB CG43722-PA CG43722-PB CG43722-PC CG43722-PD esn-PA esn-PB esn-PC esn-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42F1-42F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165507.2	NP_724533.1	espinas, isoform A
NM_001273832.1	NP_001260761.1	espinas, isoform D
NM_080251.4	NP_524990.1	espinas, isoform B
NM_001273831.1	NP_001260760.1	espinas, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Espinas (Esn), binds to an intracellular juxtamembrane domain of Flamingo (Fmi), and that this Fmi-Esn interplay elicits repulsion between dendritic branches of Drosophila sensory neurons
Title: The seven-pass transmembrane cadherin Flamingo controls dendritic self-avoidance via its binding to a LIM domain protein, Espinas, in Drosophila sensory neurons.

The following esn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the esn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11530	NM_165507.2 Latest version!	Drosophila melanogaster espinas (esn), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11530	NM_080251.4 Latest version!	Drosophila melanogaster espinas (esn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13145	NM_001273832.1 Latest version!	Drosophila melanogaster espinas (esn), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13146	NM_001273831.1 Latest version!	Drosophila melanogaster espinas (esn), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

esn ( NM_165507.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724533.1 Drosophila melanogaster espinas (esn), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11530
Clone ID Related Accession (Same CDS sequence)	NM_165507.2 , NM_080251.4
Accession Version	NM_165507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	espinas, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165507.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGG CCCCGCAGCA GCAGCAGCAT CCCCATCCGC CCAGCAGCAG CTACTACACG 
CAAACCGAAA GCGAGCTGCT GCAGATTGAG GCGGGCGGCA CGGGCCTGAC CTTCGCCTCC 
CACTCGCAAC GTCCCGAATC GGCGATCAGT CAGGTGGCAT CCACCGCCCA CCTGGACGTG 
CCCTCGGCCG CCTCCAGCGG CAGCGGGGGC AGTGCAGTCA GTGGGGGAAG CGGCGGAGCT 
CCGGAATCCG CGGGTCGATT TGTGTCCCCG CTGCAGCGCC GCCACTGCCA GCCGCCCAGC 
CACCTGCCGC TGAACTCGGT GGCCTCCCCG CTCCGCACCG CCAGCTACAA GTCGGCGGCG 
GCGGTCGCTG GTCATGGCTT CCACCACAGC CATCACCAGC AGCTGGACTT CCAGCGGAAC 
TCGCAGTCGG ACGACGACAG CGGCTGTGCC CTCGAGGAGT ACACTTGGGT GCCGCCGGGT 
CTGCGGCCCG ATCAGGTCCG TTTGTACTTC AGTCAACTGC CAGACGACAA AGTGCCCTAC 
GTCAACAGTC CCGGCGAAAA GTATCGTGTC AAGCAGTTGC TGCACCAGCT GCCGCCGCAA 
GATAACGAAG TGCGCTACTG CCACTCGCTG AGCGACGAGG AGCGCAAGGA GCTGCGCATC 
TTCTCGGCCC AAAGGAAGCG GGAGGCCCTC GGACGAGGGG CGGTCCGTCT GCTGTCCGAC 
GAGCGGCCCT GCAAGGGGTG CGAGGAGCCT TTGTCCGGCG GTGACATCGT GGTTTTCGCC 
CAGCGCCTGG GCGCCCAGTT GTGTTGGCAT CCGGGCTGCT TTGTGTGCAG CGTCTGCAAG 
GAGCTCCTGG TGGACCTCAT CTACTTCCAG CGGGACGGGA ACCTCTACTG CGGTCGGCAC 
CATGCCGAAA CCCAGAAGCC ACGCTGCTCC GCCTGCGATG AGATCATCTT CTCGGACGAG 
TGCACGGAGG CGGAGGGCAG AACGTGGCAC ATGAAGCACT TCGCCTGCCA GGAGTGCGAG 
CACCAGCTGG GCGGGCAGCG GTACATCATG CGGGAGGGCA AACCGTACTG TCTGGCCTGC 
TTCGACACAA TGTTCGCCGA GTACTGCGAC TACTGCGGGG AGGTAATCGG CGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAGCACTGG CATGCGACGG ACCAGTGCTT CAGCTGCTGC 
ACCTGCCGCT GTTCGCTGCT GGGAAGGCCC TTCCTGCCCA GAAGGGGCAC CATCTACTGC 
TCGATCGCCT GTAGCAAAGG TGAGCCGCCC ACGCCGTCAG ACACGAGTTC GGGTCCCCAG 
CTAAGGCCGA CTCACCGAGC TTCCACCTCC AGCCAAATAG CTAAATCGCC GCGTAGAGGT 
GGAGAGCGGG AGCGGGATCC GGGACGGAAG GCCCACCACG GTCACCCGAA GGCTACGGGC 
AGTGCAGGCG ATCTGCTTGA GCGGCAGGAA AGGCAGCGCA TGGAGGCCGC GGGAGTGGCG 
GACCTACTGC TCGGAGGAGG AGTACCGGGC ATGCCTCGCC CAGCCCATCC ACCGCCCATC 
GACCTAACCG AGTTGGGGAT CAGCCTGGAC AACATTTGTG CCGGCGACAA GTCCATCTTC 
GGGGACACCC AGACCCTGAC CAACTCCATG CCGGACATGC TGCTCTCCAA GGCTGACGAC 
TCGCACAGTT ACCAGAGCAT CGACAAAATC AACCTGAACT CGCCCAGCAA CTCTGATCTG 
ACGCAGAGCA CCCAGGAACT GGCCAACGAG CTGGAGCTGG ACAACGAGCC GGTGCGGGAG 
CTGCCCCACG ATGGCTACGA ACAGCTCTTC GCTAACAATC GCAACCAGGA GCACCCGGCC 
GAACAATATG ACGACGAGCA GCTGGACAAC CGACCGATGA AGGAGGTGCG CTTTCACAGC 
GTCCAGGACA CTATGTCGCG CTCAAAGAGC TACACGGATA ACTCCAATGC CCGGCGGCGG 
AGGAGGCGTC GCAACCAGTC GCGCAGCTCC TCGGAGATGC AGATCAACCA AACGAACCTC 
CGCCTGCACA ACGCACAAAC GCAGGTGGGA ACGACTCCCC TCAATCTGCT AAACAATCTG 
GACAACTGCG ACGTGGCCAG CATCTGCTCC ACCTGCTCAT CCAGCTCCTC GAGCGACATG 
GACGACTACG TATACCGCCT GCCTGCGCGG AAACACTACG GCGGAGTGAG AGTGGCCTAT 
GTGCCCAACG ATGCGTTGGC CTATGAGCGC AAGAAGAAGA TGGCCCAGGA CTCGTCCCTG 
GCCCCCGGGG CAGGCAATGC ATCCGTGGGC GGGGCACCGG CGATCATGCA CGAGAGCAAG 
AACTGCACGA TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724533.1
CDS	584..2941
Translation	MQQAPQQQQHPHPPSSSYYTQTESELLQIEAGGTGLTFASHSQRPESAISQVASTAHLDVPSAASSGSGGSAVSGGSGGAPESAGRFVSPLQRRHCQPPSHLPLNSVASPLRTASYKSAAAVAGHGFHHSHHQQLDFQRNSQSDDDSGCALEEYTWVPPGLRPDQVRLYFSQLPDDKVPYVNSPGEKYRVKQLLHQLPPQDNEVRYCHSLSDEERKELRIFSAQRKREALGRGAVRLLSDERPCKGCEEPLSGGDIVVFAQRLGAQLCWHPGCFVCSVCKELLVDLIYFQRDGNLYCGRHHAETQKPRCSACDEIIFSDECTEAEGRTWHMKHFACQECEHQLGGQRYIMREGKPYCLACFDTMFAEYCDYCGEVIGVDQGQMSHDGQHWHATDQCFSCCTCRCSLLGRPFLPRRGTIYCSIACSKGEPPTPSDTSSGPQLRPTHRASTSSQIAKSPRRGGERERDPGRKAHHGHPKATGSAGDLLERQERQRMEAAGVADLLLGGGVPGMPRPAHPPPIDLTELGISLDNICAGDKSIFGDTQTLTNSMPDMLLSKADDSHSYQSIDKINLNSPSNSDLTQSTQELANELELDNEPVRELPHDGYEQLFANNRNQEHPAEQYDDEQLDNRPMKEVRFHSVQDTMSRSKSYTDNSNARRRRRRRNQSRSSSEMQINQTNLRLHNAQTQVGTTPLNLLNNLDNCDVASICSTCSSSSSSDMDDYVYRLPARKHYGGVRVAYVPNDALAYERKKKMAQDSSLAPGAGNASVGGAPAIMHESKNCTIS

Target ORF information:

RefSeq Version	NM_165507.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster espinas (esn), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165507.2

ORF Insert Sequence:

ATGCAGCAGG CCCCGCAGCA GCAGCAGCAT CCCCATCCGC CCAGCAGCAG CTACTACACG 
CAAACCGAAA GCGAGCTGCT GCAGATTGAG GCGGGCGGCA CGGGCCTGAC CTTCGCCTCC 
CACTCGCAAC GTCCCGAATC GGCGATCAGT CAGGTGGCAT CCACCGCCCA CCTGGACGTG 
CCCTCGGCCG CCTCCAGCGG CAGCGGGGGC AGTGCAGTCA GTGGGGGAAG CGGCGGAGCT 
CCGGAATCCG CGGGTCGATT TGTGTCCCCG CTGCAGCGCC GCCACTGCCA GCCGCCCAGC 
CACCTGCCGC TGAACTCGGT GGCCTCCCCG CTCCGCACCG CCAGCTACAA GTCGGCGGCG 
GCGGTCGCTG GTCATGGCTT CCACCACAGC CATCACCAGC AGCTGGACTT CCAGCGGAAC 
TCGCAGTCGG ACGACGACAG CGGCTGTGCC CTCGAGGAGT ACACTTGGGT GCCGCCGGGT 
CTGCGGCCCG ATCAGGTCCG TTTGTACTTC AGTCAACTGC CAGACGACAA AGTGCCCTAC 
GTCAACAGTC CCGGCGAAAA GTATCGTGTC AAGCAGTTGC TGCACCAGCT GCCGCCGCAA 
GATAACGAAG TGCGCTACTG CCACTCGCTG AGCGACGAGG AGCGCAAGGA GCTGCGCATC 
TTCTCGGCCC AAAGGAAGCG GGAGGCCCTC GGACGAGGGG CGGTCCGTCT GCTGTCCGAC 
GAGCGGCCCT GCAAGGGGTG CGAGGAGCCT TTGTCCGGCG GTGACATCGT GGTTTTCGCC 
CAGCGCCTGG GCGCCCAGTT GTGTTGGCAT CCGGGCTGCT TTGTGTGCAG CGTCTGCAAG 
GAGCTCCTGG TGGACCTCAT CTACTTCCAG CGGGACGGGA ACCTCTACTG CGGTCGGCAC 
CATGCCGAAA CCCAGAAGCC ACGCTGCTCC GCCTGCGATG AGATCATCTT CTCGGACGAG 
TGCACGGAGG CGGAGGGCAG AACGTGGCAC ATGAAGCACT TCGCCTGCCA GGAGTGCGAG 
CACCAGCTGG GCGGGCAGCG GTACATCATG CGGGAGGGCA AACCGTACTG TCTGGCCTGC 
TTCGACACAA TGTTCGCCGA GTACTGCGAC TACTGCGGGG AGGTAATCGG CGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAGCACTGG CATGCGACGG ACCAGTGCTT CAGCTGCTGC 
ACCTGCCGCT GTTCGCTGCT GGGAAGGCCC TTCCTGCCCA GAAGGGGCAC CATCTACTGC 
TCGATCGCCT GTAGCAAAGG TGAGCCGCCC ACGCCGTCAG ACACGAGTTC GGGTCCCCAG 
CTAAGGCCGA CTCACCGAGC TTCCACCTCC AGCCAAATAG CTAAATCGCC GCGTAGAGGT 
GGAGAGCGGG AGCGGGATCC GGGACGGAAG GCCCACCACG GTCACCCGAA GGCTACGGGC 
AGTGCAGGCG ATCTGCTTGA GCGGCAGGAA AGGCAGCGCA TGGAGGCCGC GGGAGTGGCG 
GACCTACTGC TCGGAGGAGG AGTACCGGGC ATGCCTCGCC CAGCCCATCC ACCGCCCATC 
GACCTAACCG AGTTGGGGAT CAGCCTGGAC AACATTTGTG CCGGCGACAA GTCCATCTTC 
GGGGACACCC AGACCCTGAC CAACTCCATG CCGGACATGC TGCTCTCCAA GGCTGACGAC 
TCGCACAGTT ACCAGAGCAT CGACAAAATC AACCTGAACT CGCCCAGCAA CTCTGATCTG 
ACGCAGAGCA CCCAGGAACT GGCCAACGAG CTGGAGCTGG ACAACGAGCC GGTGCGGGAG 
CTGCCCCACG ATGGCTACGA ACAGCTCTTC GCTAACAATC GCAACCAGGA GCACCCGGCC 
GAACAATATG ACGACGAGCA GCTGGACAAC CGACCGATGA AGGAGGTGCG CTTTCACAGC 
GTCCAGGACA CTATGTCGCG CTCAAAGAGC TACACGGATA ACTCCAATGC CCGGCGGCGG 
AGGAGGCGTC GCAACCAGTC GCGCAGCTCC TCGGAGATGC AGATCAACCA AACGAACCTC 
CGCCTGCACA ACGCACAAAC GCAGGTGGGA ACGACTCCCC TCAATCTGCT AAACAATCTG 
GACAACTGCG ACGTGGCCAG CATCTGCTCC ACCTGCTCAT CCAGCTCCTC GAGCGACATG 
GACGACTACG TATACCGCCT GCCTGCGCGG AAACACTACG GCGGAGTGAG AGTGGCCTAT 
GTGCCCAACG ATGCGTTGGC CTATGAGCGC AAGAAGAAGA TGGCCCAGGA CTCGTCCCTG 
GCCCCCGGGG CAGGCAATGC ATCCGTGGGC GGGGCACCGG CGATCATGCA CGAGAGCAAG 
AACTGCACGA TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esn ( NM_080251.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524990.1 Drosophila melanogaster espinas (esn), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11530
Clone ID Related Accession (Same CDS sequence)	NM_165507.2 , NM_080251.4
Accession Version	NM_080251.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	espinas, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080251.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGG CCCCGCAGCA GCAGCAGCAT CCCCATCCGC CCAGCAGCAG CTACTACACG 
CAAACCGAAA GCGAGCTGCT GCAGATTGAG GCGGGCGGCA CGGGCCTGAC CTTCGCCTCC 
CACTCGCAAC GTCCCGAATC GGCGATCAGT CAGGTGGCAT CCACCGCCCA CCTGGACGTG 
CCCTCGGCCG CCTCCAGCGG CAGCGGGGGC AGTGCAGTCA GTGGGGGAAG CGGCGGAGCT 
CCGGAATCCG CGGGTCGATT TGTGTCCCCG CTGCAGCGCC GCCACTGCCA GCCGCCCAGC 
CACCTGCCGC TGAACTCGGT GGCCTCCCCG CTCCGCACCG CCAGCTACAA GTCGGCGGCG 
GCGGTCGCTG GTCATGGCTT CCACCACAGC CATCACCAGC AGCTGGACTT CCAGCGGAAC 
TCGCAGTCGG ACGACGACAG CGGCTGTGCC CTCGAGGAGT ACACTTGGGT GCCGCCGGGT 
CTGCGGCCCG ATCAGGTCCG TTTGTACTTC AGTCAACTGC CAGACGACAA AGTGCCCTAC 
GTCAACAGTC CCGGCGAAAA GTATCGTGTC AAGCAGTTGC TGCACCAGCT GCCGCCGCAA 
GATAACGAAG TGCGCTACTG CCACTCGCTG AGCGACGAGG AGCGCAAGGA GCTGCGCATC 
TTCTCGGCCC AAAGGAAGCG GGAGGCCCTC GGACGAGGGG CGGTCCGTCT GCTGTCCGAC 
GAGCGGCCCT GCAAGGGGTG CGAGGAGCCT TTGTCCGGCG GTGACATCGT GGTTTTCGCC 
CAGCGCCTGG GCGCCCAGTT GTGTTGGCAT CCGGGCTGCT TTGTGTGCAG CGTCTGCAAG 
GAGCTCCTGG TGGACCTCAT CTACTTCCAG CGGGACGGGA ACCTCTACTG CGGTCGGCAC 
CATGCCGAAA CCCAGAAGCC ACGCTGCTCC GCCTGCGATG AGATCATCTT CTCGGACGAG 
TGCACGGAGG CGGAGGGCAG AACGTGGCAC ATGAAGCACT TCGCCTGCCA GGAGTGCGAG 
CACCAGCTGG GCGGGCAGCG GTACATCATG CGGGAGGGCA AACCGTACTG TCTGGCCTGC 
TTCGACACAA TGTTCGCCGA GTACTGCGAC TACTGCGGGG AGGTAATCGG CGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAGCACTGG CATGCGACGG ACCAGTGCTT CAGCTGCTGC 
ACCTGCCGCT GTTCGCTGCT GGGAAGGCCC TTCCTGCCCA GAAGGGGCAC CATCTACTGC 
TCGATCGCCT GTAGCAAAGG TGAGCCGCCC ACGCCGTCAG ACACGAGTTC GGGTCCCCAG 
CTAAGGCCGA CTCACCGAGC TTCCACCTCC AGCCAAATAG CTAAATCGCC GCGTAGAGGT 
GGAGAGCGGG AGCGGGATCC GGGACGGAAG GCCCACCACG GTCACCCGAA GGCTACGGGC 
AGTGCAGGCG ATCTGCTTGA GCGGCAGGAA AGGCAGCGCA TGGAGGCCGC GGGAGTGGCG 
GACCTACTGC TCGGAGGAGG AGTACCGGGC ATGCCTCGCC CAGCCCATCC ACCGCCCATC 
GACCTAACCG AGTTGGGGAT CAGCCTGGAC AACATTTGTG CCGGCGACAA GTCCATCTTC 
GGGGACACCC AGACCCTGAC CAACTCCATG CCGGACATGC TGCTCTCCAA GGCTGACGAC 
TCGCACAGTT ACCAGAGCAT CGACAAAATC AACCTGAACT CGCCCAGCAA CTCTGATCTG 
ACGCAGAGCA CCCAGGAACT GGCCAACGAG CTGGAGCTGG ACAACGAGCC GGTGCGGGAG 
CTGCCCCACG ATGGCTACGA ACAGCTCTTC GCTAACAATC GCAACCAGGA GCACCCGGCC 
GAACAATATG ACGACGAGCA GCTGGACAAC CGACCGATGA AGGAGGTGCG CTTTCACAGC 
GTCCAGGACA CTATGTCGCG CTCAAAGAGC TACACGGATA ACTCCAATGC CCGGCGGCGG 
AGGAGGCGTC GCAACCAGTC GCGCAGCTCC TCGGAGATGC AGATCAACCA AACGAACCTC 
CGCCTGCACA ACGCACAAAC GCAGGTGGGA ACGACTCCCC TCAATCTGCT AAACAATCTG 
GACAACTGCG ACGTGGCCAG CATCTGCTCC ACCTGCTCAT CCAGCTCCTC GAGCGACATG 
GACGACTACG TATACCGCCT GCCTGCGCGG AAACACTACG GCGGAGTGAG AGTGGCCTAT 
GTGCCCAACG ATGCGTTGGC CTATGAGCGC AAGAAGAAGA TGGCCCAGGA CTCGTCCCTG 
GCCCCCGGGG CAGGCAATGC ATCCGTGGGC GGGGCACCGG CGATCATGCA CGAGAGCAAG 
AACTGCACGA TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524990.1
CDS	894..3251
Translation	MQQAPQQQQHPHPPSSSYYTQTESELLQIEAGGTGLTFASHSQRPESAISQVASTAHLDVPSAASSGSGGSAVSGGSGGAPESAGRFVSPLQRRHCQPPSHLPLNSVASPLRTASYKSAAAVAGHGFHHSHHQQLDFQRNSQSDDDSGCALEEYTWVPPGLRPDQVRLYFSQLPDDKVPYVNSPGEKYRVKQLLHQLPPQDNEVRYCHSLSDEERKELRIFSAQRKREALGRGAVRLLSDERPCKGCEEPLSGGDIVVFAQRLGAQLCWHPGCFVCSVCKELLVDLIYFQRDGNLYCGRHHAETQKPRCSACDEIIFSDECTEAEGRTWHMKHFACQECEHQLGGQRYIMREGKPYCLACFDTMFAEYCDYCGEVIGVDQGQMSHDGQHWHATDQCFSCCTCRCSLLGRPFLPRRGTIYCSIACSKGEPPTPSDTSSGPQLRPTHRASTSSQIAKSPRRGGERERDPGRKAHHGHPKATGSAGDLLERQERQRMEAAGVADLLLGGGVPGMPRPAHPPPIDLTELGISLDNICAGDKSIFGDTQTLTNSMPDMLLSKADDSHSYQSIDKINLNSPSNSDLTQSTQELANELELDNEPVRELPHDGYEQLFANNRNQEHPAEQYDDEQLDNRPMKEVRFHSVQDTMSRSKSYTDNSNARRRRRRRNQSRSSSEMQINQTNLRLHNAQTQVGTTPLNLLNNLDNCDVASICSTCSSSSSSDMDDYVYRLPARKHYGGVRVAYVPNDALAYERKKKMAQDSSLAPGAGNASVGGAPAIMHESKNCTIS

Target ORF information:

RefSeq Version	NM_080251.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster espinas (esn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080251.4

ORF Insert Sequence:

ATGCAGCAGG CCCCGCAGCA GCAGCAGCAT CCCCATCCGC CCAGCAGCAG CTACTACACG 
CAAACCGAAA GCGAGCTGCT GCAGATTGAG GCGGGCGGCA CGGGCCTGAC CTTCGCCTCC 
CACTCGCAAC GTCCCGAATC GGCGATCAGT CAGGTGGCAT CCACCGCCCA CCTGGACGTG 
CCCTCGGCCG CCTCCAGCGG CAGCGGGGGC AGTGCAGTCA GTGGGGGAAG CGGCGGAGCT 
CCGGAATCCG CGGGTCGATT TGTGTCCCCG CTGCAGCGCC GCCACTGCCA GCCGCCCAGC 
CACCTGCCGC TGAACTCGGT GGCCTCCCCG CTCCGCACCG CCAGCTACAA GTCGGCGGCG 
GCGGTCGCTG GTCATGGCTT CCACCACAGC CATCACCAGC AGCTGGACTT CCAGCGGAAC 
TCGCAGTCGG ACGACGACAG CGGCTGTGCC CTCGAGGAGT ACACTTGGGT GCCGCCGGGT 
CTGCGGCCCG ATCAGGTCCG TTTGTACTTC AGTCAACTGC CAGACGACAA AGTGCCCTAC 
GTCAACAGTC CCGGCGAAAA GTATCGTGTC AAGCAGTTGC TGCACCAGCT GCCGCCGCAA 
GATAACGAAG TGCGCTACTG CCACTCGCTG AGCGACGAGG AGCGCAAGGA GCTGCGCATC 
TTCTCGGCCC AAAGGAAGCG GGAGGCCCTC GGACGAGGGG CGGTCCGTCT GCTGTCCGAC 
GAGCGGCCCT GCAAGGGGTG CGAGGAGCCT TTGTCCGGCG GTGACATCGT GGTTTTCGCC 
CAGCGCCTGG GCGCCCAGTT GTGTTGGCAT CCGGGCTGCT TTGTGTGCAG CGTCTGCAAG 
GAGCTCCTGG TGGACCTCAT CTACTTCCAG CGGGACGGGA ACCTCTACTG CGGTCGGCAC 
CATGCCGAAA CCCAGAAGCC ACGCTGCTCC GCCTGCGATG AGATCATCTT CTCGGACGAG 
TGCACGGAGG CGGAGGGCAG AACGTGGCAC ATGAAGCACT TCGCCTGCCA GGAGTGCGAG 
CACCAGCTGG GCGGGCAGCG GTACATCATG CGGGAGGGCA AACCGTACTG TCTGGCCTGC 
TTCGACACAA TGTTCGCCGA GTACTGCGAC TACTGCGGGG AGGTAATCGG CGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAGCACTGG CATGCGACGG ACCAGTGCTT CAGCTGCTGC 
ACCTGCCGCT GTTCGCTGCT GGGAAGGCCC TTCCTGCCCA GAAGGGGCAC CATCTACTGC 
TCGATCGCCT GTAGCAAAGG TGAGCCGCCC ACGCCGTCAG ACACGAGTTC GGGTCCCCAG 
CTAAGGCCGA CTCACCGAGC TTCCACCTCC AGCCAAATAG CTAAATCGCC GCGTAGAGGT 
GGAGAGCGGG AGCGGGATCC GGGACGGAAG GCCCACCACG GTCACCCGAA GGCTACGGGC 
AGTGCAGGCG ATCTGCTTGA GCGGCAGGAA AGGCAGCGCA TGGAGGCCGC GGGAGTGGCG 
GACCTACTGC TCGGAGGAGG AGTACCGGGC ATGCCTCGCC CAGCCCATCC ACCGCCCATC 
GACCTAACCG AGTTGGGGAT CAGCCTGGAC AACATTTGTG CCGGCGACAA GTCCATCTTC 
GGGGACACCC AGACCCTGAC CAACTCCATG CCGGACATGC TGCTCTCCAA GGCTGACGAC 
TCGCACAGTT ACCAGAGCAT CGACAAAATC AACCTGAACT CGCCCAGCAA CTCTGATCTG 
ACGCAGAGCA CCCAGGAACT GGCCAACGAG CTGGAGCTGG ACAACGAGCC GGTGCGGGAG 
CTGCCCCACG ATGGCTACGA ACAGCTCTTC GCTAACAATC GCAACCAGGA GCACCCGGCC 
GAACAATATG ACGACGAGCA GCTGGACAAC CGACCGATGA AGGAGGTGCG CTTTCACAGC 
GTCCAGGACA CTATGTCGCG CTCAAAGAGC TACACGGATA ACTCCAATGC CCGGCGGCGG 
AGGAGGCGTC GCAACCAGTC GCGCAGCTCC TCGGAGATGC AGATCAACCA AACGAACCTC 
CGCCTGCACA ACGCACAAAC GCAGGTGGGA ACGACTCCCC TCAATCTGCT AAACAATCTG 
GACAACTGCG ACGTGGCCAG CATCTGCTCC ACCTGCTCAT CCAGCTCCTC GAGCGACATG 
GACGACTACG TATACCGCCT GCCTGCGCGG AAACACTACG GCGGAGTGAG AGTGGCCTAT 
GTGCCCAACG ATGCGTTGGC CTATGAGCGC AAGAAGAAGA TGGCCCAGGA CTCGTCCCTG 
GCCCCCGGGG CAGGCAATGC ATCCGTGGGC GGGGCACCGG CGATCATGCA CGAGAGCAAG 
AACTGCACGA TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esn ( NM_001273832.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260761.1 Drosophila melanogaster espinas (esn), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa13145
Clone ID Related Accession (Same CDS sequence)	NM_001273832.1
Accession Version	NM_001273832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	espinas, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGG CCCCGCAGCA GCAGCAGCAT CCCCATCCGC CCAGCAGCAG CTACTACACG 
CAAACCGAAA GCGAGCTGCT GCAGATTGAG GCGGGCGGCA CGGGCCTGAC CTTCGCCTCC 
CACTCGCAAC GTCCCGAATC GGCGATCAGT CAGGTGGCAT CCACCGCCCA CCTGGACGTG 
CCCTCGGCCG CCTCCAGCGG CAGCGGGGGC AGTGCAGTCA GTGGGGGAAG CGGCGGAGCT 
CCGGAATCCG CGGGTCGATT TGTGTCCCCG CTGCAGCGCC GCCACTGCCA GCCGCCCAGC 
CACCTGCCGC TGAACTCGGT GGCCTCCCCG CTCCGCACCG CCAGCTACAA GTCGGCGGCG 
GCGGTCGCTG GTCATGGCTT CCACCACAGC CATCACCAGC AGCTGGACTT CCAGCGGAAC 
TCGCAGTCGG ACGACGACAG CGGCTGTGCC CTCGAGGAGT ACACTTGGGT GCCGCCGGGT 
CTGCGGCCCG ATCAGGTCCG TTTGTACTTC AGTCAACTGC CAGACGACAA AGTGCCCTAC 
GTCAACAGTC CCGGCGAAAA GTATCGTGTC AAGCAGTTGC TGCACCAGCT GCCGCCGCAA 
GATAACGAAG TGCGCTACTG CCACTCGCTG AGCGACGAGG AGCGCAAGGA GCTGCGCATC 
TTCTCGGCCC AAAGGAAGCG GGAGGCCCTC GGACGAGGGG CGGTCCGTCT GCTGTCCGAC 
GAGCGGCCCT GCAAGGGGTG CGAGGAGCCT TTGTCCGGCG GTGACATCGT GGTTTTCGCC 
CAGCGCCTGG GCGCCCAGTT GTGTTGGCAT CCGGGCTGCT TTGTGTGCAG CGTCTGCAAG 
GAGCTCCTGG TGGACCTCAT CTACTTCCAG CGGGACGGGA ACCTCTACTG CGGTCGGCAC 
CATGCCGAAA CCCAGAAGCC ACGCTGCTCC GCCTGCGATG AGATCATCTT CTCGGACGAG 
TGCACGGAGG CGGAGGGCAG AACGTGGCAC ATGAAGCACT TCGCCTGCCA GGAGTGCGAG 
CACCAGCTGG GCGGGCAGCG GTACATCATG CGGGAGGGCA AACCGTACTG TCTGGCCTGC 
TTCGACACAA TGTTCGCCGA GTACTGCGAC TACTGCGGGG AGGTAATCGG CGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAGCACTGG CATGCGACGG ACCAGTGCTT CAGCTGCTGC 
ACCTGCCGCT GTTCGCTGCT GGGAAGGCCC TTCCTGCCCA GAAGGGGCAC CATCTACTGC 
TCGATCGCCT GTAGCAAAGG TGAGCCGCCC ACGCCGTCAG ACACGAGTTC GGGTCCCCAG 
CTAAGGCCGA CTCACCGAGC TTCCACCTCC AGCCAAATAG CTAAATCGCC GCGTAGAGGT 
GGAGAGCGGG AGCGGGATCC GGGACGGAAG GCCCACCACG GTCACCCGAA GGCTACGGGC 
AGTGCAGGCG ATCTGCTTGA GCGGCAGGAA AGGCAGCGCA TGGAGGCCGC GGGAGTGGCG 
GACCTACTGC TCGGAGGAGG AGTACCGGGC ATGCCTCGCC CAGCCCATCC ACCGCCCATC 
GACCTAACCG AGTTGGGGAT CAGCCTGGAC AACATTTGTG CCGGCGACAA GTCCATCTTC 
GGGGACACCC AGACCCTGAC CAACTCCATG CCGGACATGC TGCTCTCCAA GGCTGACGAC 
TCGCACAGTT ACCAGAGCAT CGACAAAATC AACCTGAACT CGCCCAGCAA CTCTGATCTG 
ACGCAGAGCA CCCAGGAACT GGCCAACGAG CTGGAGCTGG ACAACGAGCC GGTGCGGGAG 
CTGCCCCACG ATGGCTACGA ACAGCTCTTC GCTAACAATC GCAACCAGGA GCACCCGGCC 
GAACAATATG ACGACGAGCA GCTGGACAAC CGACCGATGA AGGAGGTGCG CTTTCACAGC 
GTCCAGGACA CTATGTCGCG CTCAAAGAGC TACACGGATA ACTCCAATGC CCGGCGGCGG 
AGGAGGCGTC GCAACCAGTC GCGCAGCTCC TCGGAGATGC AGATCAACCA AACGAACCTC 
CGCCTGCACA ACGCACAAAC GCAGGTGGGA ACGACTCCCC TCAATCTGCT AAACAATCTG 
GACAACTGCG ACGTGGCCAG CATCTGCTCC ACCTGCTCAT CCAGCTCCTC GAGCGACATG 
GACGACTACG TATACCGCCT GCCTGCGCGG AAACACTACG GCGGAGTGAG AGTGGCCTAT 
GTGCCCAACG ATGCGTTGGC CTATGAGCGC AAGAAGAAGA TGGCCCAGGA CTCGTCCCTG 
GCCCCCGGGG CAGGCAATGC ATCCGTGGGC GGGGCACCGG CGATCATGCA CGAGAGCAAG 
AACTGCACGA TTTCCTGACC ACCCCCCGTG CTGCCACCCC CGCCCTTCAA CCTGAGCAGC 
TACTACATCC TGGACACGAT CCTGGAGGAG CAGAGTCTGA TGCTGCCGGA GAAGAAGCCT 
GGCTGCCTGA GGAGGGTGTG GAACTTCTTT GGGCTCGGCA AGCCCCGCCA CTCCAACTCC 
CAGCACAGCA GCAACGGAAG GCTATACAGC GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260761.1
CDS	584..3139
Translation	MQQAPQQQQHPHPPSSSYYTQTESELLQIEAGGTGLTFASHSQRPESAISQVASTAHLDVPSAASSGSGGSAVSGGSGGAPESAGRFVSPLQRRHCQPPSHLPLNSVASPLRTASYKSAAAVAGHGFHHSHHQQLDFQRNSQSDDDSGCALEEYTWVPPGLRPDQVRLYFSQLPDDKVPYVNSPGEKYRVKQLLHQLPPQDNEVRYCHSLSDEERKELRIFSAQRKREALGRGAVRLLSDERPCKGCEEPLSGGDIVVFAQRLGAQLCWHPGCFVCSVCKELLVDLIYFQRDGNLYCGRHHAETQKPRCSACDEIIFSDECTEAEGRTWHMKHFACQECEHQLGGQRYIMREGKPYCLACFDTMFAEYCDYCGEVIGVDQGQMSHDGQHWHATDQCFSCCTCRCSLLGRPFLPRRGTIYCSIACSKGEPPTPSDTSSGPQLRPTHRASTSSQIAKSPRRGGERERDPGRKAHHGHPKATGSAGDLLERQERQRMEAAGVADLLLGGGVPGMPRPAHPPPIDLTELGISLDNICAGDKSIFGDTQTLTNSMPDMLLSKADDSHSYQSIDKINLNSPSNSDLTQSTQELANELELDNEPVRELPHDGYEQLFANNRNQEHPAEQYDDEQLDNRPMKEVRFHSVQDTMSRSKSYTDNSNARRRRRRRNQSRSSSEMQINQTNLRLHNAQTQVGTTPLNLLNNLDNCDVASICSTCSSSSSSDMDDYVYRLPARKHYGGVRVAYVPNDALAYERKKKMAQDSSLAPGAGNASVGGAPAIMHESKNCTIS*PPPVLPPPPFNLSSYYILDTILEEQSLMLPEKKPGCLRRVWNFFGLGKPRHSNSQHSSNGRLYSV

Target ORF information:

RefSeq Version	NM_001273832.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster espinas (esn), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273832.1

ORF Insert Sequence:

ATGCAGCAGG CCCCGCAGCA GCAGCAGCAT CCCCATCCGC CCAGCAGCAG CTACTACACG 
CAAACCGAAA GCGAGCTGCT GCAGATTGAG GCGGGCGGCA CGGGCCTGAC CTTCGCCTCC 
CACTCGCAAC GTCCCGAATC GGCGATCAGT CAGGTGGCAT CCACCGCCCA CCTGGACGTG 
CCCTCGGCCG CCTCCAGCGG CAGCGGGGGC AGTGCAGTCA GTGGGGGAAG CGGCGGAGCT 
CCGGAATCCG CGGGTCGATT TGTGTCCCCG CTGCAGCGCC GCCACTGCCA GCCGCCCAGC 
CACCTGCCGC TGAACTCGGT GGCCTCCCCG CTCCGCACCG CCAGCTACAA GTCGGCGGCG 
GCGGTCGCTG GTCATGGCTT CCACCACAGC CATCACCAGC AGCTGGACTT CCAGCGGAAC 
TCGCAGTCGG ACGACGACAG CGGCTGTGCC CTCGAGGAGT ACACTTGGGT GCCGCCGGGT 
CTGCGGCCCG ATCAGGTCCG TTTGTACTTC AGTCAACTGC CAGACGACAA AGTGCCCTAC 
GTCAACAGTC CCGGCGAAAA GTATCGTGTC AAGCAGTTGC TGCACCAGCT GCCGCCGCAA 
GATAACGAAG TGCGCTACTG CCACTCGCTG AGCGACGAGG AGCGCAAGGA GCTGCGCATC 
TTCTCGGCCC AAAGGAAGCG GGAGGCCCTC GGACGAGGGG CGGTCCGTCT GCTGTCCGAC 
GAGCGGCCCT GCAAGGGGTG CGAGGAGCCT TTGTCCGGCG GTGACATCGT GGTTTTCGCC 
CAGCGCCTGG GCGCCCAGTT GTGTTGGCAT CCGGGCTGCT TTGTGTGCAG CGTCTGCAAG 
GAGCTCCTGG TGGACCTCAT CTACTTCCAG CGGGACGGGA ACCTCTACTG CGGTCGGCAC 
CATGCCGAAA CCCAGAAGCC ACGCTGCTCC GCCTGCGATG AGATCATCTT CTCGGACGAG 
TGCACGGAGG CGGAGGGCAG AACGTGGCAC ATGAAGCACT TCGCCTGCCA GGAGTGCGAG 
CACCAGCTGG GCGGGCAGCG GTACATCATG CGGGAGGGCA AACCGTACTG TCTGGCCTGC 
TTCGACACAA TGTTCGCCGA GTACTGCGAC TACTGCGGGG AGGTAATCGG CGTGGACCAG 
GGCCAGATGA GCCACGACGG GCAGCACTGG CATGCGACGG ACCAGTGCTT CAGCTGCTGC 
ACCTGCCGCT GTTCGCTGCT GGGAAGGCCC TTCCTGCCCA GAAGGGGCAC CATCTACTGC 
TCGATCGCCT GTAGCAAAGG TGAGCCGCCC ACGCCGTCAG ACACGAGTTC GGGTCCCCAG 
CTAAGGCCGA CTCACCGAGC TTCCACCTCC AGCCAAATAG CTAAATCGCC GCGTAGAGGT 
GGAGAGCGGG AGCGGGATCC GGGACGGAAG GCCCACCACG GTCACCCGAA GGCTACGGGC 
AGTGCAGGCG ATCTGCTTGA GCGGCAGGAA AGGCAGCGCA TGGAGGCCGC GGGAGTGGCG 
GACCTACTGC TCGGAGGAGG AGTACCGGGC ATGCCTCGCC CAGCCCATCC ACCGCCCATC 
GACCTAACCG AGTTGGGGAT CAGCCTGGAC AACATTTGTG CCGGCGACAA GTCCATCTTC 
GGGGACACCC AGACCCTGAC CAACTCCATG CCGGACATGC TGCTCTCCAA GGCTGACGAC 
TCGCACAGTT ACCAGAGCAT CGACAAAATC AACCTGAACT CGCCCAGCAA CTCTGATCTG 
ACGCAGAGCA CCCAGGAACT GGCCAACGAG CTGGAGCTGG ACAACGAGCC GGTGCGGGAG 
CTGCCCCACG ATGGCTACGA ACAGCTCTTC GCTAACAATC GCAACCAGGA GCACCCGGCC 
GAACAATATG ACGACGAGCA GCTGGACAAC CGACCGATGA AGGAGGTGCG CTTTCACAGC 
GTCCAGGACA CTATGTCGCG CTCAAAGAGC TACACGGATA ACTCCAATGC CCGGCGGCGG 
AGGAGGCGTC GCAACCAGTC GCGCAGCTCC TCGGAGATGC AGATCAACCA AACGAACCTC 
CGCCTGCACA ACGCACAAAC GCAGGTGGGA ACGACTCCCC TCAATCTGCT AAACAATCTG 
GACAACTGCG ACGTGGCCAG CATCTGCTCC ACCTGCTCAT CCAGCTCCTC GAGCGACATG 
GACGACTACG TATACCGCCT GCCTGCGCGG AAACACTACG GCGGAGTGAG AGTGGCCTAT 
GTGCCCAACG ATGCGTTGGC CTATGAGCGC AAGAAGAAGA TGGCCCAGGA CTCGTCCCTG 
GCCCCCGGGG CAGGCAATGC ATCCGTGGGC GGGGCACCGG CGATCATGCA CGAGAGCAAG 
AACTGCACGA TTTCCTGACC ACCCCCCGTG CTGCCACCCC CGCCCTTCAA CCTGAGCAGC 
TACTACATCC TGGACACGAT CCTGGAGGAG CAGAGTCTGA TGCTGCCGGA GAAGAAGCCT 
GGCTGCCTGA GGAGGGTGTG GAACTTCTTT GGGCTCGGCA AGCCCCGCCA CTCCAACTCC 
CAGCACAGCA GCAACGGAAG GCTATACAGC GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

esn ( NM_001273831.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260760.1 Drosophila melanogaster espinas (esn), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13146
Clone ID Related Accession (Same CDS sequence)	NM_001273831.1
Accession Version	NM_001273831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	espinas, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTCGA AGAAGCGCTG GTGGAAGATC CGGCTGCCGC AGGTGGGCAA GCAGGATCAG 
TCGAGGAACT ACCGGGACAT CGTCAACCTG AGCGAGTGCT CCTCAGTGCT GGCCAACACG 
GCCACCACGA TTAGCGACAG CTCCACCACC ACCGCCAGCA GCGGCAGTCC CACCCGCCAC 
CGGAGCTGCG ACAGTCTCTC CAAGAGCTCC CAGGCGGAGG CGGGCAAGCT GTTCCCCCGG 
AACATGCCCA GCATACGGCG GAGTCGCCGA CGGGCTGTGT CCGCGGAAAG GGAGCGCCTG 
GCCAGCCGGG AGCGGCTGGA GGAGCAGGTT CAGCCGCCGA AAATGCCGCA GATAGGAGCT 
GGACAGCAGT CCCGGCTATC GACGGTGACC AAACTGACGC CCAGGAAGCA GAGTAGCAAG 
TCCACGTGCT CCAAACATCG GGGCAACGCC CCTGCGAAGT GCATCATCAA GTCGGCAGTG 
CGAACCAAGA CCAAGCCAAA GGCCACCACG ATCACCCTAG CACCCTACGA AATAGCGCTG 
GAGCTGCCCA ACGAATCCCC TGCACCCACT TTGGCCATGG CCGTGGCCCA ATCCTCCGGC 
TGCGTGCAGA GGAAGCTGAG CAGCGAGGAT CAGTTCCACT CCTCGGGAAT CTCCTGCAAC 
GGGGAGACCG ACGATGACGT GGAGGTGGCC ATGCTGACCG GCAGCTTAAC GCCCTCCCAG 
ATAGCAAGGA TCCAGGGGCA GCAGATCCTC CGATATGACG ATCTGAGGAA CCTGAACCGC 
TGCCGCAACA GGAACGCCGT TTGGCTAAAT CCCAAGGATA CGGAGCAGCT CCTCGAGGTG 
GACAAGAGCA GGAGGAAGGC CAACTGCTAC TTCAACCAGG CCATTGCCGA CGATATCCAG 
CTCAGCGATA TTGAAGAACA ATTGGCCAGA TTTGATGGCC ACTCAAAGAG GCCGCCACCG 
CCGGCAGCCA CAGCACCCAA AATCACCCAG CCCAGCCAAG TAATCGAAAA ATTCAAGCCC 
ACGCCCGCAC CCCGCTCTTC CGGCAACATG CAGCAGGCCC CGCAGCAGCA GCAGCATCCC 
CATCCGCCCA GCAGCAGCTA CTACACGCAA ACCGAAAGCG AGCTGCTGCA GATTGAGGCG 
GGCGGCACGG GCCTGACCTT CGCCTCCCAC TCGCAACGTC CCGAATCGGC GATCAGTCAG 
GTGGCATCCA CCGCCCACCT GGACGTGCCC TCGGCCGCCT CCAGCGGCAG CGGGGGCAGT 
GCAGTCAGTG GGGGAAGCGG CGGAGCTCCG GAATCCGCGG GTCGATTTGT GTCCCCGCTG 
CAGCGCCGCC ACTGCCAGCC GCCCAGCCAC CTGCCGCTGA ACTCGGTGGC CTCCCCGCTC 
CGCACCGCCA GCTACAAGTC GGCGGCGGCG GTCGCTGGTC ATGGCTTCCA CCACAGCCAT 
CACCAGCAGC TGGACTTCCA GCGGAACTCG CAGTCGGACG ACGACAGCGG CTGTGCCCTC 
GAGGAGTACA CTTGGGTGCC GCCGGGTCTG CGGCCCGATC AGGTCCGTTT GTACTTCAGT 
CAACTGCCAG ACGACAAAGT GCCCTACGTC AACAGTCCCG GCGAAAAGTA TCGTGTCAAG 
CAGTTGCTGC ACCAGCTGCC GCCGCAAGAT AACGAAGTGC GCTACTGCCA CTCGCTGAGC 
GACGAGGAGC GCAAGGAGCT GCGCATCTTC TCGGCCCAAA GGAAGCGGGA GGCCCTCGGA 
CGAGGGGCGG TCCGTCTGCT GTCCGACGAG CGGCCCTGCA AGGGGTGCGA GGAGCCTTTG 
TCCGGCGGTG ACATCGTGGT TTTCGCCCAG CGCCTGGGCG CCCAGTTGTG TTGGCATCCG 
GGCTGCTTTG TGTGCAGCGT CTGCAAGGAG CTCCTGGTGG ACCTCATCTA CTTCCAGCGG 
GACGGGAACC TCTACTGCGG TCGGCACCAT GCCGAAACCC AGAAGCCACG CTGCTCCGCC 
TGCGATGAGA TCATCTTCTC GGACGAGTGC ACGGAGGCGG AGGGCAGAAC GTGGCACATG 
AAGCACTTCG CCTGCCAGGA GTGCGAGCAC CAGCTGGGCG GGCAGCGGTA CATCATGCGG 
GAGGGCAAAC CGTACTGTCT GGCCTGCTTC GACACAATGT TCGCCGAGTA CTGCGACTAC 
TGCGGGGAGG TAATCGGCGT GGACCAGGGC CAGATGAGCC ACGACGGGCA GCACTGGCAT 
GCGACGGACC AGTGCTTCAG CTGCTGCACC TGCCGCTGTT CGCTGCTGGG AAGGCCCTTC 
CTGCCCAGAA GGGGCACCAT CTACTGCTCG ATCGCCTGTA GCAAAGGTGA GCCGCCCACG 
CCGTCAGACA CGAGTTCGGG TCCCCAGCTA AGGCCGACTC ACCGAGCTTC CACCTCCAGC 
CAAATAGCTA AATCGCCGCG TAGAGGTGGA GAGCGGGAGC GGGATCCGGG ACGGAAGGCC 
CACCACGGTC ACCCGAAGGC TACGGGCAGT GCAGGCGATC TGCTTGAGCG GCAGGAAAGG 
CAGCGCATGG AGGCCGCGGG AGTGGCGGAC CTACTGCTCG GAGGAGGAGT ACCGGGCATG 
CCTCGCCCAG CCCATCCACC GCCCATCGAC CTAACCGAGT TGGGGATCAG CCTGGACAAC 
ATTTGTGCCG GCGACAAGTC CATCTTCGGG GACACCCAGA CCCTGACCAA CTCCATGCCG 
GACATGCTGC TCTCCAAGGC TGACGACTCG CACAGTTACC AGAGCATCGA CAAAATCAAC 
CTGAACTCGC CCAGCAACTC TGATCTGACG CAGAGCACCC AGGAACTGGC CAACGAGCTG 
GAGCTGGACA ACGAGCCGGT GCGGGAGCTG CCCCACGATG GCTACGAACA GCTCTTCGCT 
AACAATCGCA ACCAGGAGCA CCCGGCCGAA CAATATGACG ACGAGCAGCT GGACAACCGA 
CCGATGAAGG AGGTGCGCTT TCACAGCGTC CAGGACACTA TGTCGCGCTC AAAGAGCTAC 
ACGGATAACT CCAATGCCCG GCGGCGGAGG AGGCGTCGCA ACCAGTCGCG CAGCTCCTCG 
GAGATGCAGA TCAACCAAAC GAACCTCCGC CTGCACAACG CACAAACGCA GGTGGGAACG 
ACTCCCCTCA ATCTGCTAAA CAATCTGGAC AACTGCGACG TGGCCAGCAT CTGCTCCACC 
TGCTCATCCA GCTCCTCGAG CGACATGGAC GACTACGTAT ACCGCCTGCC TGCGCGGAAA 
CACTACGGCG GAGTGAGAGT GGCCTATGTG CCCAACGATG CGTTGGCCTA TGAGCGCAAG 
AAGAAGATGG CCCAGGACTC GTCCCTGGCC CCCGGGGCAG GCAATGCATC CGTGGGCGGG 
GCACCGGCGA TCATGCACGA GAGCAAGAAC TGCACGATTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260760.1
CDS	263..3667
Translation	MLSKKRWWKIRLPQVGKQDQSRNYRDIVNLSECSSVLANTATTISDSSTTTASSGSPTRHRSCDSLSKSSQAEAGKLFPRNMPSIRRSRRRAVSAERERLASRERLEEQVQPPKMPQIGAGQQSRLSTVTKLTPRKQSSKSTCSKHRGNAPAKCIIKSAVRTKTKPKATTITLAPYEIALELPNESPAPTLAMAVAQSSGCVQRKLSSEDQFHSSGISCNGETDDDVEVAMLTGSLTPSQIARIQGQQILRYDDLRNLNRCRNRNAVWLNPKDTEQLLEVDKSRRKANCYFNQAIADDIQLSDIEEQLARFDGHSKRPPPPAATAPKITQPSQVIEKFKPTPAPRSSGNMQQAPQQQQHPHPPSSSYYTQTESELLQIEAGGTGLTFASHSQRPESAISQVASTAHLDVPSAASSGSGGSAVSGGSGGAPESAGRFVSPLQRRHCQPPSHLPLNSVASPLRTASYKSAAAVAGHGFHHSHHQQLDFQRNSQSDDDSGCALEEYTWVPPGLRPDQVRLYFSQLPDDKVPYVNSPGEKYRVKQLLHQLPPQDNEVRYCHSLSDEERKELRIFSAQRKREALGRGAVRLLSDERPCKGCEEPLSGGDIVVFAQRLGAQLCWHPGCFVCSVCKELLVDLIYFQRDGNLYCGRHHAETQKPRCSACDEIIFSDECTEAEGRTWHMKHFACQECEHQLGGQRYIMREGKPYCLACFDTMFAEYCDYCGEVIGVDQGQMSHDGQHWHATDQCFSCCTCRCSLLGRPFLPRRGTIYCSIACSKGEPPTPSDTSSGPQLRPTHRASTSSQIAKSPRRGGERERDPGRKAHHGHPKATGSAGDLLERQERQRMEAAGVADLLLGGGVPGMPRPAHPPPIDLTELGISLDNICAGDKSIFGDTQTLTNSMPDMLLSKADDSHSYQSIDKINLNSPSNSDLTQSTQELANELELDNEPVRELPHDGYEQLFANNRNQEHPAEQYDDEQLDNRPMKEVRFHSVQDTMSRSKSYTDNSNARRRRRRRNQSRSSSEMQINQTNLRLHNAQTQVGTTPLNLLNNLDNCDVASICSTCSSSSSSDMDDYVYRLPARKHYGGVRVAYVPNDALAYERKKKMAQDSSLAPGAGNASVGGAPAIMHESKNCTIS

Target ORF information:

RefSeq Version	NM_001273831.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster espinas (esn), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273831.1

ORF Insert Sequence:

ATGCTCTCGA AGAAGCGCTG GTGGAAGATC CGGCTGCCGC AGGTGGGCAA GCAGGATCAG 
TCGAGGAACT ACCGGGACAT CGTCAACCTG AGCGAGTGCT CCTCAGTGCT GGCCAACACG 
GCCACCACGA TTAGCGACAG CTCCACCACC ACCGCCAGCA GCGGCAGTCC CACCCGCCAC 
CGGAGCTGCG ACAGTCTCTC CAAGAGCTCC CAGGCGGAGG CGGGCAAGCT GTTCCCCCGG 
AACATGCCCA GCATACGGCG GAGTCGCCGA CGGGCTGTGT CCGCGGAAAG GGAGCGCCTG 
GCCAGCCGGG AGCGGCTGGA GGAGCAGGTT CAGCCGCCGA AAATGCCGCA GATAGGAGCT 
GGACAGCAGT CCCGGCTATC GACGGTGACC AAACTGACGC CCAGGAAGCA GAGTAGCAAG 
TCCACGTGCT CCAAACATCG GGGCAACGCC CCTGCGAAGT GCATCATCAA GTCGGCAGTG 
CGAACCAAGA CCAAGCCAAA GGCCACCACG ATCACCCTAG CACCCTACGA AATAGCGCTG 
GAGCTGCCCA ACGAATCCCC TGCACCCACT TTGGCCATGG CCGTGGCCCA ATCCTCCGGC 
TGCGTGCAGA GGAAGCTGAG CAGCGAGGAT CAGTTCCACT CCTCGGGAAT CTCCTGCAAC 
GGGGAGACCG ACGATGACGT GGAGGTGGCC ATGCTGACCG GCAGCTTAAC GCCCTCCCAG 
ATAGCAAGGA TCCAGGGGCA GCAGATCCTC CGATATGACG ATCTGAGGAA CCTGAACCGC 
TGCCGCAACA GGAACGCCGT TTGGCTAAAT CCCAAGGATA CGGAGCAGCT CCTCGAGGTG 
GACAAGAGCA GGAGGAAGGC CAACTGCTAC TTCAACCAGG CCATTGCCGA CGATATCCAG 
CTCAGCGATA TTGAAGAACA ATTGGCCAGA TTTGATGGCC ACTCAAAGAG GCCGCCACCG 
CCGGCAGCCA CAGCACCCAA AATCACCCAG CCCAGCCAAG TAATCGAAAA ATTCAAGCCC 
ACGCCCGCAC CCCGCTCTTC CGGCAACATG CAGCAGGCCC CGCAGCAGCA GCAGCATCCC 
CATCCGCCCA GCAGCAGCTA CTACACGCAA ACCGAAAGCG AGCTGCTGCA GATTGAGGCG 
GGCGGCACGG GCCTGACCTT CGCCTCCCAC TCGCAACGTC CCGAATCGGC GATCAGTCAG 
GTGGCATCCA CCGCCCACCT GGACGTGCCC TCGGCCGCCT CCAGCGGCAG CGGGGGCAGT 
GCAGTCAGTG GGGGAAGCGG CGGAGCTCCG GAATCCGCGG GTCGATTTGT GTCCCCGCTG 
CAGCGCCGCC ACTGCCAGCC GCCCAGCCAC CTGCCGCTGA ACTCGGTGGC CTCCCCGCTC 
CGCACCGCCA GCTACAAGTC GGCGGCGGCG GTCGCTGGTC ATGGCTTCCA CCACAGCCAT 
CACCAGCAGC TGGACTTCCA GCGGAACTCG CAGTCGGACG ACGACAGCGG CTGTGCCCTC 
GAGGAGTACA CTTGGGTGCC GCCGGGTCTG CGGCCCGATC AGGTCCGTTT GTACTTCAGT 
CAACTGCCAG ACGACAAAGT GCCCTACGTC AACAGTCCCG GCGAAAAGTA TCGTGTCAAG 
CAGTTGCTGC ACCAGCTGCC GCCGCAAGAT AACGAAGTGC GCTACTGCCA CTCGCTGAGC 
GACGAGGAGC GCAAGGAGCT GCGCATCTTC TCGGCCCAAA GGAAGCGGGA GGCCCTCGGA 
CGAGGGGCGG TCCGTCTGCT GTCCGACGAG CGGCCCTGCA AGGGGTGCGA GGAGCCTTTG 
TCCGGCGGTG ACATCGTGGT TTTCGCCCAG CGCCTGGGCG CCCAGTTGTG TTGGCATCCG 
GGCTGCTTTG TGTGCAGCGT CTGCAAGGAG CTCCTGGTGG ACCTCATCTA CTTCCAGCGG 
GACGGGAACC TCTACTGCGG TCGGCACCAT GCCGAAACCC AGAAGCCACG CTGCTCCGCC 
TGCGATGAGA TCATCTTCTC GGACGAGTGC ACGGAGGCGG AGGGCAGAAC GTGGCACATG 
AAGCACTTCG CCTGCCAGGA GTGCGAGCAC CAGCTGGGCG GGCAGCGGTA CATCATGCGG 
GAGGGCAAAC CGTACTGTCT GGCCTGCTTC GACACAATGT TCGCCGAGTA CTGCGACTAC 
TGCGGGGAGG TAATCGGCGT GGACCAGGGC CAGATGAGCC ACGACGGGCA GCACTGGCAT 
GCGACGGACC AGTGCTTCAG CTGCTGCACC TGCCGCTGTT CGCTGCTGGG AAGGCCCTTC 
CTGCCCAGAA GGGGCACCAT CTACTGCTCG ATCGCCTGTA GCAAAGGTGA GCCGCCCACG 
CCGTCAGACA CGAGTTCGGG TCCCCAGCTA AGGCCGACTC ACCGAGCTTC CACCTCCAGC 
CAAATAGCTA AATCGCCGCG TAGAGGTGGA GAGCGGGAGC GGGATCCGGG ACGGAAGGCC 
CACCACGGTC ACCCGAAGGC TACGGGCAGT GCAGGCGATC TGCTTGAGCG GCAGGAAAGG 
CAGCGCATGG AGGCCGCGGG AGTGGCGGAC CTACTGCTCG GAGGAGGAGT ACCGGGCATG 
CCTCGCCCAG CCCATCCACC GCCCATCGAC CTAACCGAGT TGGGGATCAG CCTGGACAAC 
ATTTGTGCCG GCGACAAGTC CATCTTCGGG GACACCCAGA CCCTGACCAA CTCCATGCCG 
GACATGCTGC TCTCCAAGGC TGACGACTCG CACAGTTACC AGAGCATCGA CAAAATCAAC 
CTGAACTCGC CCAGCAACTC TGATCTGACG CAGAGCACCC AGGAACTGGC CAACGAGCTG 
GAGCTGGACA ACGAGCCGGT GCGGGAGCTG CCCCACGATG GCTACGAACA GCTCTTCGCT 
AACAATCGCA ACCAGGAGCA CCCGGCCGAA CAATATGACG ACGAGCAGCT GGACAACCGA 
CCGATGAAGG AGGTGCGCTT TCACAGCGTC CAGGACACTA TGTCGCGCTC AAAGAGCTAC 
ACGGATAACT CCAATGCCCG GCGGCGGAGG AGGCGTCGCA ACCAGTCGCG CAGCTCCTCG 
GAGATGCAGA TCAACCAAAC GAACCTCCGC CTGCACAACG CACAAACGCA GGTGGGAACG 
ACTCCCCTCA ATCTGCTAAA CAATCTGGAC AACTGCGACG TGGCCAGCAT CTGCTCCACC 
TGCTCATCCA GCTCCTCGAG CGACATGGAC GACTACGTAT ACCGCCTGCC TGCGCGGAAA 
CACTACGGCG GAGTGAGAGT GGCCTATGTG CCCAACGATG CGTTGGCCTA TGAGCGCAAG 
AAGAAGATGG CCCAGGACTC GTCCCTGGCC CCCGGGGCAG GCAATGCATC CGTGGGCGGG 
GCACCGGCGA TCATGCACGA GAGCAAGAAC TGCACGATTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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esn cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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