SF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SF2
Entrez Gene ID	53443
Full Name	Splicing factor 2
Synonyms	154911_at,ASF,ASF/SF2,CG6987,Dmel\CG6987,SRp30,cg6987,dASF,dSF2/ASF,dSRp30,dmSF2/p28
General protein information	Preferred Names Splicing factor 2 Names CG6987 gene product from transcript CG6987-RB CG6987-PA CG6987-PB SF2-PA SF2-PB SRp30 splicing factor 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B13-89B13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260210.2	NP_001247139.1	splicing factor 2, isoform B
NM_144354.3	NP_652611.1	splicing factor 2, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SRSF1	NP_001182476.1
Gallus gallus (chicken)	SRSF1	NP_001107213.1
Xenopus tropicalis (tropical clawed frog)	srsf1	NP_001006919.1
Homo sapiens (human)	SRSF1	NP_008855.1
Danio rerio (zebrafish)	srsf1a	NP_998180.1
Drosophila melanogaster (fruit fly)	SF2	NP_652611.1
Mus musculus (house mouse)	Srsf1	NP_775550.2
Arabidopsis thaliana (thale cress)	SR1	NP_850934.1
Canis lupus familiaris (dog)	SRSF1	XP_005624772.1
Bos taurus (cattle)	SRSF1	NP_001069862.1
	SRSF1	XP_001103473.2
Rattus norvegicus (Norway rat)	Srsf1	NP_001103022.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010496	XP_314469.4
Caenorhabditis elegans (roundworm)	rsp-3	NP_499649.2
Arabidopsis thaliana (thale cress)	AT4G02430	NP_567235.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila splicing factor SF2 knock-down mutant shows altered cell-cycle in vivo
Title: Drosophila splicing factor SF2 knock-down mutant shows altered cell-cycle in vivo.

The SR proteins dASF/SF2 and B52 caused defects in ommatidia structure, and B52 impaired normal axonal projections of photoreceptors and neurogenesis in visual ganglia.
Title: The SR family proteins B52 and dASF/SF2 modulate development of the Drosophila visual system by regulating specific RNA targets.

The following SF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07014	NM_001260210.2 Latest version!	Drosophila melanogaster splicing factor 2, transcript variant B (SF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa07014	NM_144354.3 Latest version!	Drosophila melanogaster splicing factor 2, transcript variant A (SF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SF2 ( NM_001260210.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247139.1 Drosophila melanogaster splicing factor 2, transcript variant B (SF2), mRNA.

Price & Availability↑

CloneID	OFa07014
Clone ID Related Accession (Same CDS sequence)	NM_001260210.2 , NM_144354.3
Accession Version	NM_001260210.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260210.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGATCAC GCAACGAGTG CCGTATATAT GTGGGCAACC TTCCCCCAGA TATACGCACC 
AAGGATATCC AGGACCTGTT CCACAAGTTC GGCAAAGTAA CTTTCGTCGA TCTGAAAAAT 
CGGCGTGGGC CGCCATTTGC TTTTGTTGAG TTCGAAGATG CGCGGGATGC CGACGATGCG 
GTGAAGGCGC GCGACGGCTA CGACTACGAT GGGTATCGTC TGCGCGTGGA GTTCCCGCGG 
GGCGGTGGTC CTGGAAGCTA CCGCGGCGGC AACCGCAATG ACCGAAGCCG CGACGGTGGG 
GGACGGATGG GCGGACGCGG ACCGCCAGCC AAGCGCTCGC AGTACCGCGT CATGGTTACT 
GGACTGCCCG CCTCCGGATC GTGGCAAGAT CTCAAGGATC ACATGCGCGA GGCCGGCGAC 
GTCTGCTTCG CGGACACTTA CAAGGATGGT TCCGGCGTCG TTGAGTTCCT GCGCCACGAG 
GACATGAAGT ACGCAATCAA AAAATTGGAC GACTCTCGCT TCCGATCGCA TGAGGGCGAG 
GTTGCCTACA TTCGCGTACG CGAGGATAGC GGTGATAACG ACAGAGGCGG TGGTGGTGGC 
GGCAGCGGTG GAGGTGGTGG CGGCAGCGGC GGAGGTGGAA GCCGTGACTA CCGCGACAGA 
TCCCGTTCGC GCTCATTCTC GTCGAGGCCG CGCCGTCGTG GCACTCCCAC ATACTCCCCA 
GTGCGACGTC AATCTTATTC CAGGTCTCGC TCACGTTCTA ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247139.1
CDS	98..865
Translation	MGSRNECRIYVGNLPPDIRTKDIQDLFHKFGKVTFVDLKNRRGPPFAFVEFEDARDADDAVKARDGYDYDGYRLRVEFPRGGGPGSYRGGNRNDRSRDGGGRMGGRGPPAKRSQYRVMVTGLPASGSWQDLKDHMREAGDVCFADTYKDGSGVVEFLRHEDMKYAIKKLDDSRFRSHEGEVAYIRVREDSGDNDRGGGGGGSGGGGGGSGGGGSRDYRDRSRSRSFSSRPRRRGTPTYSPVRRQSYSRSRSRSNY

Target ORF information:

RefSeq Version	NM_001260210.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 2, transcript variant B (SF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260210.2

ORF Insert Sequence:

ATGGGATCAC GCAACGAGTG CCGTATATAT GTGGGCAACC TTCCCCCAGA TATACGCACC 
AAGGATATCC AGGACCTGTT CCACAAGTTC GGCAAAGTAA CTTTCGTCGA TCTGAAAAAT 
CGGCGTGGGC CGCCATTTGC TTTTGTTGAG TTCGAAGATG CGCGGGATGC CGACGATGCG 
GTGAAGGCGC GCGACGGCTA CGACTACGAT GGGTATCGTC TGCGCGTGGA GTTCCCGCGG 
GGCGGTGGTC CTGGAAGCTA CCGCGGCGGC AACCGCAATG ACCGAAGCCG CGACGGTGGG 
GGACGGATGG GCGGACGCGG ACCGCCAGCC AAGCGCTCGC AGTACCGCGT CATGGTTACT 
GGACTGCCCG CCTCCGGATC GTGGCAAGAT CTCAAGGATC ACATGCGCGA GGCCGGCGAC 
GTCTGCTTCG CGGACACTTA CAAGGATGGT TCCGGCGTCG TTGAGTTCCT GCGCCACGAG 
GACATGAAGT ACGCAATCAA AAAATTGGAC GACTCTCGCT TCCGATCGCA TGAGGGCGAG 
GTTGCCTACA TTCGCGTACG CGAGGATAGC GGTGATAACG ACAGAGGCGG TGGTGGTGGC 
GGCAGCGGTG GAGGTGGTGG CGGCAGCGGC GGAGGTGGAA GCCGTGACTA CCGCGACAGA 
TCCCGTTCGC GCTCATTCTC GTCGAGGCCG CGCCGTCGTG GCACTCCCAC ATACTCCCCA 
GTGCGACGTC AATCTTATTC CAGGTCTCGC TCACGTTCTA ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF2 ( NM_144354.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652611.1 Drosophila melanogaster splicing factor 2, transcript variant A (SF2), mRNA.

Price & Availability↑

CloneID	OFa07014
Clone ID Related Accession (Same CDS sequence)	NM_001260210.2 , NM_144354.3
Accession Version	NM_144354.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_144354.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGATCAC GCAACGAGTG CCGTATATAT GTGGGCAACC TTCCCCCAGA TATACGCACC 
AAGGATATCC AGGACCTGTT CCACAAGTTC GGCAAAGTAA CTTTCGTCGA TCTGAAAAAT 
CGGCGTGGGC CGCCATTTGC TTTTGTTGAG TTCGAAGATG CGCGGGATGC CGACGATGCG 
GTGAAGGCGC GCGACGGCTA CGACTACGAT GGGTATCGTC TGCGCGTGGA GTTCCCGCGG 
GGCGGTGGTC CTGGAAGCTA CCGCGGCGGC AACCGCAATG ACCGAAGCCG CGACGGTGGG 
GGACGGATGG GCGGACGCGG ACCGCCAGCC AAGCGCTCGC AGTACCGCGT CATGGTTACT 
GGACTGCCCG CCTCCGGATC GTGGCAAGAT CTCAAGGATC ACATGCGCGA GGCCGGCGAC 
GTCTGCTTCG CGGACACTTA CAAGGATGGT TCCGGCGTCG TTGAGTTCCT GCGCCACGAG 
GACATGAAGT ACGCAATCAA AAAATTGGAC GACTCTCGCT TCCGATCGCA TGAGGGCGAG 
GTTGCCTACA TTCGCGTACG CGAGGATAGC GGTGATAACG ACAGAGGCGG TGGTGGTGGC 
GGCAGCGGTG GAGGTGGTGG CGGCAGCGGC GGAGGTGGAA GCCGTGACTA CCGCGACAGA 
TCCCGTTCGC GCTCATTCTC GTCGAGGCCG CGCCGTCGTG GCACTCCCAC ATACTCCCCA 
GTGCGACGTC AATCTTATTC CAGGTCTCGC TCACGTTCTA ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652611.1
CDS	98..865
Translation	MGSRNECRIYVGNLPPDIRTKDIQDLFHKFGKVTFVDLKNRRGPPFAFVEFEDARDADDAVKARDGYDYDGYRLRVEFPRGGGPGSYRGGNRNDRSRDGGGRMGGRGPPAKRSQYRVMVTGLPASGSWQDLKDHMREAGDVCFADTYKDGSGVVEFLRHEDMKYAIKKLDDSRFRSHEGEVAYIRVREDSGDNDRGGGGGGSGGGGGGSGGGGSRDYRDRSRSRSFSSRPRRRGTPTYSPVRRQSYSRSRSRSNY

Target ORF information:

RefSeq Version	NM_144354.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 2, transcript variant A (SF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144354.3

ORF Insert Sequence:

ATGGGATCAC GCAACGAGTG CCGTATATAT GTGGGCAACC TTCCCCCAGA TATACGCACC 
AAGGATATCC AGGACCTGTT CCACAAGTTC GGCAAAGTAA CTTTCGTCGA TCTGAAAAAT 
CGGCGTGGGC CGCCATTTGC TTTTGTTGAG TTCGAAGATG CGCGGGATGC CGACGATGCG 
GTGAAGGCGC GCGACGGCTA CGACTACGAT GGGTATCGTC TGCGCGTGGA GTTCCCGCGG 
GGCGGTGGTC CTGGAAGCTA CCGCGGCGGC AACCGCAATG ACCGAAGCCG CGACGGTGGG 
GGACGGATGG GCGGACGCGG ACCGCCAGCC AAGCGCTCGC AGTACCGCGT CATGGTTACT 
GGACTGCCCG CCTCCGGATC GTGGCAAGAT CTCAAGGATC ACATGCGCGA GGCCGGCGAC 
GTCTGCTTCG CGGACACTTA CAAGGATGGT TCCGGCGTCG TTGAGTTCCT GCGCCACGAG 
GACATGAAGT ACGCAATCAA AAAATTGGAC GACTCTCGCT TCCGATCGCA TGAGGGCGAG 
GTTGCCTACA TTCGCGTACG CGAGGATAGC GGTGATAACG ACAGAGGCGG TGGTGGTGGC 
GGCAGCGGTG GAGGTGGTGG CGGCAGCGGC GGAGGTGGAA GCCGTGACTA CCGCGACAGA 
TCCCGTTCGC GCTCATTCTC GTCGAGGCCG CGCCGTCGTG GCACTCCCAC ATACTCCCCA 
GTGCGACGTC AATCTTATTC CAGGTCTCGC TCACGTTCTA ACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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