RecQ4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RecQ4
Entrez Gene ID	53438
Full Name	RecQ4 helicase
Synonyms	CG7487,DmRECQ4,Dmel\CG7487,RECQL4,cg7487,dRecQ4
General protein information	Preferred Names RecQ4 helicase Names CG7487 gene product from transcript CG7487-RA CG7487-PA RecQ4-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66B10-66B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_144350.2	NP_652607.1	RecQ4 helicase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RecQ4	NP_652607.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005917	XP_315948.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recq4 is essential for viability.
Title: Drosophila RecQ4 has a 3'-5' DNA helicase activity that is essential for viability.

RecQ4 is required for most aspects of DNA replication during development.
Title: Drosophila homologue of the Rothmund-Thomson syndrome gene: essential function in DNA replication during development.

The following RecQ4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RecQ4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20296	NM_144350.2 Latest version!	Drosophila melanogaster RecQ4 helicase (RecQ4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RecQ4 ( NM_144350.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652607.1 Drosophila melanogaster RecQ4 helicase (RecQ4), mRNA.

Price & Availability↑

CloneID	OFa20296
Clone ID Related Accession (Same CDS sequence)	NM_144350.2
Accession Version	NM_144350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RecQ4 helicase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_144350.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGATT CGGTGTTCAA GCTAAAATAC CAAAAGTACA AGTTGCGCGT CAAGGTGTGG 
GAGAAGGACT TCAAGAAGAA GAATGGCCGT GTTCCATCGA AGTATGATAT AAGGGACGCC 
AGTCAGGAGA TTCGGGACTC ATACAAAATG TACTACAAAC TGAAGACATC CTTCCTGGAA 
GAGACGCTGA ACGATGTTCT CAGCGAAGAT GGATACGATA TTCTGGAGAT GTCGCAGGCC 
AGTGATTTCG GTGTGAGCAT GCTGGACCAG GACGTCAGTC TTAATGAAGG TCCTCAGCTA 
CCGTTGGATA TATCTGCACT GGTGGGGCCG CAGAGCTCTG GTAATCTTGA GGAAATTCCA 
CAATCGGTGG AGGGCTCATT CTCAAATCTC ATCGACCTGC CCAATCGTCA AGTACTCACC 
AATCTGGTCA ATCGCGATGA AAACCATGTC ATTCGCAAAT TCGAGGCGGT GGAGGAGCTG 
CCAATCAACC AAAATGCCTG GGGCTTGAAT GTGAGTAAGA AGCCACCAGC TCCACCACAG 
CCTGTAGAGG CCTCCAAATC CGCTCCGGGG CACGGAAAGC AACCCAAAGC GGGCGCTAGT 
CTTAAGCCCA GCTTGAGTGC CAAGTTATTC CAATCCTCAC GCGGTTTTGC TAAACGAAAT 
CCCCGAAAGC CGCTTTCACG TTGTGTCAGT TCCTCTAGTT CCACCACATC ACTTAGCTCA 
GTTCCTACTG ATCATCACGA GGAACTGCTC GACTTTGAGA CCATACTCAT ACGCAAAGCC 
CAGGAGTACA AGGAGAAGCA GCAGGCCATA GCTAACAATC CAATGCTGGC CAGTGAGCAC 
AGCAAGGAAA GTATCAAAAC ACTGGTGGAC GACGGCTGGC TCCGGCGAAA TACAAAGGAA 
AACACTCTTG ATGAAGAAGT GCCCTTTGCG GAAGCAAATA ATAACAGTGG TACGTCCAAG 
AAAACAAACT TTGGCCTGGC CAATCTTGAT TTAAGTAAAC TAAAGCCCAC TGTTAAGGAG 
GAAAAAGTGT TGCAGGCCAA GCCCGATCAA ATGGCAATCA TACAGGAGCT GCAAACAGAT 
ATGAATTCTA GCATGAACCA GAAACCAGAT CATCTTAATC ACACACCTCC TGCATCCAGT 
CAAAAATCAG TCGCTCCTAA AAATAAACCT CCACCTTCCG AGCAGGAAAC CGATTCGGAT 
TCCGACTCTG TTGTGGCTGA GAGTGAAGAA GAGCAGGAAC CACAAGAATA TCGGCAACTA 
TCAAAACGTC GCAAAATTGT TTCAACAGCA TCAGGAAAAG TGGAAGTAGC CGCCCCAGTT 
GAAATTCCCA ACAAAGTCGA ACCAGAGACA GAAACATTTG CTCAAGAGAA TCCAGATTTC 
TCTGCAGATG AAGACCAGGA TGCTACATAT GTACCAGAGA ATAAAAAGAA AGATAAGGCA 
AAGCGCAAGC AAGCCGCCGG AAAACAAAAG ACCACTAAGC CCAAAGCGGA ACCAAAACCG 
AAAACAGAAA AGAAAGCCAA AGTCAAGGCC GAAAAGAAAC CCAAGGCGGA GAAAAAGCCG 
AGGAACTCTA AAAAAGCCAT CGCTGTCGAG CCGGCTCCTG ATCCAGAAGA GGATGAGCGC 
CAACCATTGA ATCCAGAAGA TTTGAAATAC GTGTTGGCCT TGGAGGCGGG AGATATTACC 
TCCGTTCCGC GCATTAATGT GCAGGATCTA GAAGAAGCGG ATGCTACCGC CCAGCGCTAT 
ATTAGAACCT TCGCCGCTGG ACCCAACCCA GGATTGTCGG AAGGGTCCAA CATTCGAGTG 
GATGAAAAGA GAGCCGCAGC ACGAAAGAAG CTGGAGGAAC GCATAGCAGC TGGTAAATTA 
AACGAGAACT TTGTGACCAT AAATATTCAA AAGAAAAAAT TCGTGCGTGG CAAAAAGTCC 
GTGAACTTCA GCAAGTACAA GAAGCAACAG TGGCGGCATA AGAAACGAGT GGCTGCCTTG 
AGTGGGCCGG ACATGGATAT GGGTGGCTGT GATGGCGGAG TGCTCACGTG CTTCCAGTGC 
GGCGGAGTCG GGCATTTCGC TCAGCAGTGC AAGGTGAAGG GAGACAGCTT GTTGCCACTT 
TCGGCACAGC TGGAAGAGGA TCCCTCGCCG TTTCCCACGC TTGCCGAGGC TCAGGAGATG 
GCCAGTCAGG GAGCAGTGGT GGCACACAGT CGCAACATCT CACGGCTGCC CCAAGCGGCC 
AATGCAGCCA TCCTGCAAGG CGATGAGTTG AACGAGTCAG AGGAAGATGA CCAAGAAAGC 
AGCGGAGATG AGGAGGTACA GCAGCATCCA GATCCAAATT GGTCTAGCGA TGAAATGGAC 
GTTGATTTCG AGGCCCTAGA TGCGGCCGTC GAAGCTTCCC TAAGCCAACC GGTTTCGCAA 
GAGAAAGCAG CTTCACCTAT TAAGACATAC GTCGGCCACA AGATTCCCGA GGAGTTCCTC 
AAGCAGGCTG GACTGGACAC CACAGCCTCT AGTTCGAATC GCAGCCAGCA CGGTGGGGTG 
AAGCCCCTAT ACGATCTCCT TCCGGATGGC AGTGTCCAGG ACACGACGCC AGAGGTCCTA 
GAGGCACTCC ACATGTTCGG GCACACCAAC TTCAGGAAAG GTCAAGATCG CGCCATCATG 
CGCACTCTCT CTGGACTATC ATCGCTAGTT ACACTTAGCA CGGGCAGCGG CAAATCTTTG 
TGCTACCAAT TGCCCGCCTA TTTGTATAGC CGGAAAGTGG GAGCTATTAC CCTAGTCATC 
TCACCATTGG TGTCACTTAT GGAGGATCAG GTGACCGGGG TGCCGCACTT TCTGCGAGCT 
CACTGCCTGC ACACTAATCA GACCGCGCCG CAGCGTATGA AGATTCAGCA GATGATTGCC 
AATGGGGAGA TTGACATTCT TCTTGTCTCT CCGGAGGCGG TTGTCGCCGG GGAACGAGCC 
ACAGGATTTG GAGCCATTCT TCGCCAGCTG CCGCCCATTG CATTCGCTTG TATTGATGAG 
GCGCATTGCG TGTCTCAGTG GAGTCACAAC TTTCGACCCA GCTACCTAAT GATCTGCAAG 
GTGCTGAGAA AGAATCTCGG TGTACGCACA GTGCTGGGGC TAACAGCCAC GGCAACTTTG 
CCAACGCGTG TTAGCATTAT AAATCACCTT GGCATATCGG ATGGAGAACG TGGTATTATA 
AGCGACATCC CACTGCCTGA CAATCTAGTG CTGTCCGTCT CCAAGGATGA GAACCGGGAT 
GCAGCTCTGC TCCAACTGCT CAATAGCGAA CGCTTCGAGC CCTGCCAGTC CATTATTATC 
TATTGTACGC GTCGTGACGA GTGTGAGCGT ATCGCTGGGT TTATTAGAAC GTGCGTGCAA 
GACCGTAGGG AGCCAACTCA GGATCAGACG AAAAAACGTA AACGTGTGAA TTGGCAGGCT 
GAGCCCTATC ACGCTGGAAT GCCCGCTTCC AGGCGACGAA CCGTTCAGAA GGCCTTCATG 
AGCAATGAGC TGCGCATTGT GGTGGCCACC ATTGCCTTTG GTATGGGCAT CAATAAGCCG 
GACATACGCG CGGTAATCCA TTATAATATG CCAAGAAACT TTGAGAGCTA TGTGCAGGAA 
ATTGGTCGAG CAGGACGCGA CGGGTTGCCT TCCCACTGTC ACCTCTTCTT GGATGCGAAG 
GGTGGCGACC AGAGTGAGCT GCGTCGCCAT GTGTATTCTA ACTCCATTGA TCGGCATGTT 
ATCCGTAAGC TGCTGCAAAA GATCTTTGTG CCATGTAGCT GTGACAAGGA GGCTTCCAAG 
AGGACAGCTC TTCCGATTCC CCTTGAAGGT GATGGTCCTA GAGTGCATAT GTGTCCGGGA 
CACGAGATTG GCTTTTCCGT GGAGAAAACC GTTGAGATGC TGGACATCCC GGCGGAGAAC 
ATATCTACTT TGCTCTGCTA CATGGAGCTT GATCCCCGAT GGTGCATCAG TGTGCTCAGC 
TCAGCATATG TGATGGCTAA GGTAATCTCG TATGGTGGGC CAAAGTACTT GAAGCATGCT 
GCAAAGGAAT GTCCACCATT GGCCATGGCT ATTGCTTTGC AGATTAGAGA TAAGACATTC 
AAGGAGGACT CTAACATCAT AGAATTCTCG GTCACAGATA TCGCGGCAGG AATTGGCTGG 
AACAGTGGTG TGGTCAAATA CCAACTGAAG GATCTGGAGT GGGTAAAAGT AAATGGATAT 
CCCAAGCGAT CGCCCATCAC GGTGAGCTTC TATGATTTGG GATTCCGCAT CAAGGTGCCG 
GGCGATTTTA CCGAATCAGA GATCGACAAC GCTCTGGACA CTCTCTATAC GCGATCCGTG 
AAACAGGAGC GTACGCAGCT CATCCAGCTG CAATATGTGG CCCATGGATT GGCTGCTGTG 
GCCTACAGCT CTTGTGGCCA GTGCTGCAAT GCGGATTTTC CGCAGGATCG GGGCGAGCAG 
CTAAAGGCTA TCGTGCGCAA CTACTTTGCC AATGACTACC CACAGGATCT GGAGCTGGAA 
ATAGAGCCAA GTAACGTGCC GGATGAGAAC ATCATAGACG ACGTTCATGC TCTAATAAAC 
ATGTACCCGG ACAATACATT TACCGGACGT AATATTGCGC GCATCTTTCA TGGCATCATG 
AGCCCGAACT ATCCGGCTGT CATCTGGGGA CGCTGTAAGT TCTGGCGTGC CCACGTCAAA 
GTGGATTTCA ATCGCATCCT GCACCTGGCC AATATGGCTA TTATCAAGAG GCGTACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652607.1
CDS	123..4862
Translation	MDDSVFKLKYQKYKLRVKVWEKDFKKKNGRVPSKYDIRDASQEIRDSYKMYYKLKTSFLEETLNDVLSEDGYDILEMSQASDFGVSMLDQDVSLNEGPQLPLDISALVGPQSSGNLEEIPQSVEGSFSNLIDLPNRQVLTNLVNRDENHVIRKFEAVEELPINQNAWGLNVSKKPPAPPQPVEASKSAPGHGKQPKAGASLKPSLSAKLFQSSRGFAKRNPRKPLSRCVSSSSSTTSLSSVPTDHHEELLDFETILIRKAQEYKEKQQAIANNPMLASEHSKESIKTLVDDGWLRRNTKENTLDEEVPFAEANNNSGTSKKTNFGLANLDLSKLKPTVKEEKVLQAKPDQMAIIQELQTDMNSSMNQKPDHLNHTPPASSQKSVAPKNKPPPSEQETDSDSDSVVAESEEEQEPQEYRQLSKRRKIVSTASGKVEVAAPVEIPNKVEPETETFAQENPDFSADEDQDATYVPENKKKDKAKRKQAAGKQKTTKPKAEPKPKTEKKAKVKAEKKPKAEKKPRNSKKAIAVEPAPDPEEDERQPLNPEDLKYVLALEAGDITSVPRINVQDLEEADATAQRYIRTFAAGPNPGLSEGSNIRVDEKRAAARKKLEERIAAGKLNENFVTINIQKKKFVRGKKSVNFSKYKKQQWRHKKRVAALSGPDMDMGGCDGGVLTCFQCGGVGHFAQQCKVKGDSLLPLSAQLEEDPSPFPTLAEAQEMASQGAVVAHSRNISRLPQAANAAILQGDELNESEEDDQESSGDEEVQQHPDPNWSSDEMDVDFEALDAAVEASLSQPVSQEKAASPIKTYVGHKIPEEFLKQAGLDTTASSSNRSQHGGVKPLYDLLPDGSVQDTTPEVLEALHMFGHTNFRKGQDRAIMRTLSGLSSLVTLSTGSGKSLCYQLPAYLYSRKVGAITLVISPLVSLMEDQVTGVPHFLRAHCLHTNQTAPQRMKIQQMIANGEIDILLVSPEAVVAGERATGFGAILRQLPPIAFACIDEAHCVSQWSHNFRPSYLMICKVLRKNLGVRTVLGLTATATLPTRVSIINHLGISDGERGIISDIPLPDNLVLSVSKDENRDAALLQLLNSERFEPCQSIIIYCTRRDECERIAGFIRTCVQDRREPTQDQTKKRKRVNWQAEPYHAGMPASRRRTVQKAFMSNELRIVVATIAFGMGINKPDIRAVIHYNMPRNFESYVQEIGRAGRDGLPSHCHLFLDAKGGDQSELRRHVYSNSIDRHVIRKLLQKIFVPCSCDKEASKRTALPIPLEGDGPRVHMCPGHEIGFSVEKTVEMLDIPAENISTLLCYMELDPRWCISVLSSAYVMAKVISYGGPKYLKHAAKECPPLAMAIALQIRDKTFKEDSNIIEFSVTDIAAGIGWNSGVVKYQLKDLEWVKVNGYPKRSPITVSFYDLGFRIKVPGDFTESEIDNALDTLYTRSVKQERTQLIQLQYVAHGLAAVAYSSCGQCCNADFPQDRGEQLKAIVRNYFANDYPQDLELEIEPSNVPDENIIDDVHALINMYPDNTFTGRNIARIFHGIMSPNYPAVIWGRCKFWRAHVKVDFNRILHLANMAIIKRRT

Target ORF information:

RefSeq Version	NM_144350.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RecQ4 helicase (RecQ4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144350.2

ORF Insert Sequence:

ATGGACGATT CGGTGTTCAA GCTAAAATAC CAAAAGTACA AGTTGCGCGT CAAGGTGTGG 
GAGAAGGACT TCAAGAAGAA GAATGGCCGT GTTCCATCGA AGTATGATAT AAGGGACGCC 
AGTCAGGAGA TTCGGGACTC ATACAAAATG TACTACAAAC TGAAGACATC CTTCCTGGAA 
GAGACGCTGA ACGATGTTCT CAGCGAAGAT GGATACGATA TTCTGGAGAT GTCGCAGGCC 
AGTGATTTCG GTGTGAGCAT GCTGGACCAG GACGTCAGTC TTAATGAAGG TCCTCAGCTA 
CCGTTGGATA TATCTGCACT GGTGGGGCCG CAGAGCTCTG GTAATCTTGA GGAAATTCCA 
CAATCGGTGG AGGGCTCATT CTCAAATCTC ATCGACCTGC CCAATCGTCA AGTACTCACC 
AATCTGGTCA ATCGCGATGA AAACCATGTC ATTCGCAAAT TCGAGGCGGT GGAGGAGCTG 
CCAATCAACC AAAATGCCTG GGGCTTGAAT GTGAGTAAGA AGCCACCAGC TCCACCACAG 
CCTGTAGAGG CCTCCAAATC CGCTCCGGGG CACGGAAAGC AACCCAAAGC GGGCGCTAGT 
CTTAAGCCCA GCTTGAGTGC CAAGTTATTC CAATCCTCAC GCGGTTTTGC TAAACGAAAT 
CCCCGAAAGC CGCTTTCACG TTGTGTCAGT TCCTCTAGTT CCACCACATC ACTTAGCTCA 
GTTCCTACTG ATCATCACGA GGAACTGCTC GACTTTGAGA CCATACTCAT ACGCAAAGCC 
CAGGAGTACA AGGAGAAGCA GCAGGCCATA GCTAACAATC CAATGCTGGC CAGTGAGCAC 
AGCAAGGAAA GTATCAAAAC ACTGGTGGAC GACGGCTGGC TCCGGCGAAA TACAAAGGAA 
AACACTCTTG ATGAAGAAGT GCCCTTTGCG GAAGCAAATA ATAACAGTGG TACGTCCAAG 
AAAACAAACT TTGGCCTGGC CAATCTTGAT TTAAGTAAAC TAAAGCCCAC TGTTAAGGAG 
GAAAAAGTGT TGCAGGCCAA GCCCGATCAA ATGGCAATCA TACAGGAGCT GCAAACAGAT 
ATGAATTCTA GCATGAACCA GAAACCAGAT CATCTTAATC ACACACCTCC TGCATCCAGT 
CAAAAATCAG TCGCTCCTAA AAATAAACCT CCACCTTCCG AGCAGGAAAC CGATTCGGAT 
TCCGACTCTG TTGTGGCTGA GAGTGAAGAA GAGCAGGAAC CACAAGAATA TCGGCAACTA 
TCAAAACGTC GCAAAATTGT TTCAACAGCA TCAGGAAAAG TGGAAGTAGC CGCCCCAGTT 
GAAATTCCCA ACAAAGTCGA ACCAGAGACA GAAACATTTG CTCAAGAGAA TCCAGATTTC 
TCTGCAGATG AAGACCAGGA TGCTACATAT GTACCAGAGA ATAAAAAGAA AGATAAGGCA 
AAGCGCAAGC AAGCCGCCGG AAAACAAAAG ACCACTAAGC CCAAAGCGGA ACCAAAACCG 
AAAACAGAAA AGAAAGCCAA AGTCAAGGCC GAAAAGAAAC CCAAGGCGGA GAAAAAGCCG 
AGGAACTCTA AAAAAGCCAT CGCTGTCGAG CCGGCTCCTG ATCCAGAAGA GGATGAGCGC 
CAACCATTGA ATCCAGAAGA TTTGAAATAC GTGTTGGCCT TGGAGGCGGG AGATATTACC 
TCCGTTCCGC GCATTAATGT GCAGGATCTA GAAGAAGCGG ATGCTACCGC CCAGCGCTAT 
ATTAGAACCT TCGCCGCTGG ACCCAACCCA GGATTGTCGG AAGGGTCCAA CATTCGAGTG 
GATGAAAAGA GAGCCGCAGC ACGAAAGAAG CTGGAGGAAC GCATAGCAGC TGGTAAATTA 
AACGAGAACT TTGTGACCAT AAATATTCAA AAGAAAAAAT TCGTGCGTGG CAAAAAGTCC 
GTGAACTTCA GCAAGTACAA GAAGCAACAG TGGCGGCATA AGAAACGAGT GGCTGCCTTG 
AGTGGGCCGG ACATGGATAT GGGTGGCTGT GATGGCGGAG TGCTCACGTG CTTCCAGTGC 
GGCGGAGTCG GGCATTTCGC TCAGCAGTGC AAGGTGAAGG GAGACAGCTT GTTGCCACTT 
TCGGCACAGC TGGAAGAGGA TCCCTCGCCG TTTCCCACGC TTGCCGAGGC TCAGGAGATG 
GCCAGTCAGG GAGCAGTGGT GGCACACAGT CGCAACATCT CACGGCTGCC CCAAGCGGCC 
AATGCAGCCA TCCTGCAAGG CGATGAGTTG AACGAGTCAG AGGAAGATGA CCAAGAAAGC 
AGCGGAGATG AGGAGGTACA GCAGCATCCA GATCCAAATT GGTCTAGCGA TGAAATGGAC 
GTTGATTTCG AGGCCCTAGA TGCGGCCGTC GAAGCTTCCC TAAGCCAACC GGTTTCGCAA 
GAGAAAGCAG CTTCACCTAT TAAGACATAC GTCGGCCACA AGATTCCCGA GGAGTTCCTC 
AAGCAGGCTG GACTGGACAC CACAGCCTCT AGTTCGAATC GCAGCCAGCA CGGTGGGGTG 
AAGCCCCTAT ACGATCTCCT TCCGGATGGC AGTGTCCAGG ACACGACGCC AGAGGTCCTA 
GAGGCACTCC ACATGTTCGG GCACACCAAC TTCAGGAAAG GTCAAGATCG CGCCATCATG 
CGCACTCTCT CTGGACTATC ATCGCTAGTT ACACTTAGCA CGGGCAGCGG CAAATCTTTG 
TGCTACCAAT TGCCCGCCTA TTTGTATAGC CGGAAAGTGG GAGCTATTAC CCTAGTCATC 
TCACCATTGG TGTCACTTAT GGAGGATCAG GTGACCGGGG TGCCGCACTT TCTGCGAGCT 
CACTGCCTGC ACACTAATCA GACCGCGCCG CAGCGTATGA AGATTCAGCA GATGATTGCC 
AATGGGGAGA TTGACATTCT TCTTGTCTCT CCGGAGGCGG TTGTCGCCGG GGAACGAGCC 
ACAGGATTTG GAGCCATTCT TCGCCAGCTG CCGCCCATTG CATTCGCTTG TATTGATGAG 
GCGCATTGCG TGTCTCAGTG GAGTCACAAC TTTCGACCCA GCTACCTAAT GATCTGCAAG 
GTGCTGAGAA AGAATCTCGG TGTACGCACA GTGCTGGGGC TAACAGCCAC GGCAACTTTG 
CCAACGCGTG TTAGCATTAT AAATCACCTT GGCATATCGG ATGGAGAACG TGGTATTATA 
AGCGACATCC CACTGCCTGA CAATCTAGTG CTGTCCGTCT CCAAGGATGA GAACCGGGAT 
GCAGCTCTGC TCCAACTGCT CAATAGCGAA CGCTTCGAGC CCTGCCAGTC CATTATTATC 
TATTGTACGC GTCGTGACGA GTGTGAGCGT ATCGCTGGGT TTATTAGAAC GTGCGTGCAA 
GACCGTAGGG AGCCAACTCA GGATCAGACG AAAAAACGTA AACGTGTGAA TTGGCAGGCT 
GAGCCCTATC ACGCTGGAAT GCCCGCTTCC AGGCGACGAA CCGTTCAGAA GGCCTTCATG 
AGCAATGAGC TGCGCATTGT GGTGGCCACC ATTGCCTTTG GTATGGGCAT CAATAAGCCG 
GACATACGCG CGGTAATCCA TTATAATATG CCAAGAAACT TTGAGAGCTA TGTGCAGGAA 
ATTGGTCGAG CAGGACGCGA CGGGTTGCCT TCCCACTGTC ACCTCTTCTT GGATGCGAAG 
GGTGGCGACC AGAGTGAGCT GCGTCGCCAT GTGTATTCTA ACTCCATTGA TCGGCATGTT 
ATCCGTAAGC TGCTGCAAAA GATCTTTGTG CCATGTAGCT GTGACAAGGA GGCTTCCAAG 
AGGACAGCTC TTCCGATTCC CCTTGAAGGT GATGGTCCTA GAGTGCATAT GTGTCCGGGA 
CACGAGATTG GCTTTTCCGT GGAGAAAACC GTTGAGATGC TGGACATCCC GGCGGAGAAC 
ATATCTACTT TGCTCTGCTA CATGGAGCTT GATCCCCGAT GGTGCATCAG TGTGCTCAGC 
TCAGCATATG TGATGGCTAA GGTAATCTCG TATGGTGGGC CAAAGTACTT GAAGCATGCT 
GCAAAGGAAT GTCCACCATT GGCCATGGCT ATTGCTTTGC AGATTAGAGA TAAGACATTC 
AAGGAGGACT CTAACATCAT AGAATTCTCG GTCACAGATA TCGCGGCAGG AATTGGCTGG 
AACAGTGGTG TGGTCAAATA CCAACTGAAG GATCTGGAGT GGGTAAAAGT AAATGGATAT 
CCCAAGCGAT CGCCCATCAC GGTGAGCTTC TATGATTTGG GATTCCGCAT CAAGGTGCCG 
GGCGATTTTA CCGAATCAGA GATCGACAAC GCTCTGGACA CTCTCTATAC GCGATCCGTG 
AAACAGGAGC GTACGCAGCT CATCCAGCTG CAATATGTGG CCCATGGATT GGCTGCTGTG 
GCCTACAGCT CTTGTGGCCA GTGCTGCAAT GCGGATTTTC CGCAGGATCG GGGCGAGCAG 
CTAAAGGCTA TCGTGCGCAA CTACTTTGCC AATGACTACC CACAGGATCT GGAGCTGGAA 
ATAGAGCCAA GTAACGTGCC GGATGAGAAC ATCATAGACG ACGTTCATGC TCTAATAAAC 
ATGTACCCGG ACAATACATT TACCGGACGT AATATTGCGC GCATCTTTCA TGGCATCATG 
AGCCCGAACT ATCCGGCTGT CATCTGGGGA CGCTGTAAGT TCTGGCGTGC CCACGTCAAA 
GTGGATTTCA ATCGCATCCT GCACCTGGCC AATATGGCTA TTATCAAGAG GCGTACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RecQ4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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