Best1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Best1
Entrez Gene ID	53431
Full Name	Bestrophin 1
Synonyms	CG6264,Dbest,Dmel\CG6264,anon-WO0118547.380,best,dBest1,dbest1,dmBest1
General protein information	Preferred Names Bestrophin 1 Names CG6264 gene product from transcript CG6264-RB Best1-PA Best1-PB Best1-PC Best1-PD CG6264-PA CG6264-PB CG6264-PC CG6264-PD bestrophin 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85F15-85F16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275523.1	NP_001262452.1	bestrophin 1, isoform D
NM_144346.4	NP_652603.1	bestrophin 1, isoform A
NM_001260089.2	NP_001247018.1	bestrophin 1, isoform C
NM_001144561.2	NP_001138033.1	bestrophin 1, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BEST2	XP_002688807.1
Mus musculus (house mouse)	Best2	NP_001123666.1
Rattus norvegicus (Norway rat)	Best2	NP_001102365.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011745	XP_320767.3
	BEST2	XP_001108800.1
Canis lupus familiaris (dog)	BEST2	XP_003639774.1
Drosophila melanogaster (fruit fly)	Best1	NP_652603.1
Pan troglodytes (chimpanzee)	BEST2	XP_512414.2
Danio rerio (zebrafish)	best2	XP_695597.1
Caenorhabditis elegans (roundworm)	best-23	NP_001122668.1
Xenopus tropicalis (tropical clawed frog)	best2	NP_988974.1
Homo sapiens (human)	BEST2	NP_060152.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bestrophin 1 (dBest1) as the Drosophila Cl(swell) channel
Title: Anion-sensitive fluorophore identifies the Drosophila swell-activated chloride channel in a genome-wide RNA interference screen.

bestrophin-1 chloride current is dually regulated by calcium and cell volume
Title: Drosophila bestrophin-1 chloride current is dually regulated by calcium and cell volume.

Here it is reported that bestrophin1 is a component of native Calcium channel at the plasma membrane.
Title: Single Cl- channels activated by Ca2+ in Drosophila S2 cells are mediated by bestrophins.

Using heterologous expression, we show here that human, Drosophila, and C. elegans bestrophins form oligomeric chloride channels, and that human bestrophin is sensitive to intracellular calcium
Title: The vitelliform macular dystrophy protein defines a new family of chloride channels.

The following Best1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Best1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07010	NM_001144561.2 Latest version!	Drosophila melanogaster bestrophin 1, transcript variant B (Best1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa07011	NM_001275523.1 Latest version!	Drosophila melanogaster bestrophin 1, transcript variant D (Best1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa07012	NM_001260089.2 Latest version!	Drosophila melanogaster bestrophin 1, transcript variant C (Best1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08744	NM_144346.4 Latest version!	Drosophila melanogaster bestrophin 1, transcript variant A (Best1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Best1 ( NM_001144561.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138033.1 Drosophila melanogaster bestrophin 1, transcript variant B (Best1), mRNA.

Price & Availability↑

CloneID	OFa07010
Clone ID Related Accession (Same CDS sequence)	NM_001144561.2
Accession Version	NM_001144561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bestrophin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144561.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGTTGGTAA TGCTAATGGT 
GATCCCGCCG CCGCTGCGGA TGCGAAAGCC CCGAACCCGG TTACGGGTCA AGTTCAAGTT 
CCGGGTGCGG ACACCGGCGT CCAAACCGTT ACTTATGATT TTCCCAAGGG CTCGCCAGAT 
GAGGAGGCCG ATGATGCCAG TGGCATTCAC TTCTCAGCTG GAAATGGCAA AATGCGCCTG 
GATTCCTCGC CCTCGCTGGT GAGCGTTTCG GGAACTCTAT CCCGGGTGAA TACGGTGGCC 
TCGGCCCTCA AACGTTTCCT GAGCCGCGAC GATAGCAGGC CGGGATCGGC AACGCCCAGT 
CAGGATCAGC CCTACAAATT CCCGGCCAGC GCCAGCTCGG CGAGTTTATC GGGTGCCGTG 
GTAGGATCGG CTACATCGGC CGGAAAACCA GCTGGCAGTC TTAGGATTAC GCAGCAAGTG 
ATCGAGGAGG TGGACGAACA GGCGACCATA ACATCCATGA GAGCCAATGA TCCACGTCCC 
AATGTCATGG ACATATTCGC ACAAACCTCG TCGGGAGCTG GAACCTCTGG ACCGCTGCAG 
CCACCACCGG CCCACTCGGA ACCGGTGGAC ATCCCGTCAC GTCCGCCCTC ATACAATCGG 
GCCCAATCCC AGTACGAACC CAACCTATTT CCACCTGGCG GAGTGGATGC ACTGCTCAGT 
ACTTCAGCTC CTGCGGGCGG AAGTCCCCTG CTCCTGTCTA ATGCAGCCAC TGCACCCAGT 
TCGCCAGTGG GCGAGAGCTC CAAGTCCCTA TACGATCCAC AAAAGGGCGC CAGCCGAGAG 
ACAGTGGAGA GCATGGACCT GAGGTCCTCC ACGGATCTAC TCGGCGATGC GGCAGTGCAG 
CCCGAAGACG AGGGCGATGA CTTCGATAAG CTGAAGGCGG AACGCGAGAA GGAGAAACTG 
ATGCGACAGC AAAAGAATCT GGCCAGAACT ATTAGCACCG CTCCGGGAAT GGAAGCCACG 
GCTGTGCCGA TGGTGCCAAT GGTCCCAGTG AACGTGGCAG TGCAACAGGC ACAGCTGCAA 
CCAGTTGCAT CCAGTGCCGA TCTTCTGGCC GGCGGAGATC AGTTCTCCAA TTCGACGATG 
AAATCGGAGG ACGCCATCAA CGGCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138033.1
CDS	439..2748
Translation	MTITYTGEVATCRGFGCFLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGLNPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDERGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDNEKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELLKDQYWDEVFPNELPYTIAAERFRENHPEPSTAKIEVPKNAAMPSTMSSVRIDEMVGNANGDPAAAADAKAPNPVTGQVQVPGADTGVQTVTYDFPKGSPDEEADDASGIHFSAGNGKMRLDSSPSLVSVSGTLSRVNTVASALKRFLSRDDSRPGSATPSQDQPYKFPASASSASLSGAVVGSATSAGKPAGSLRITQQVIEEVDEQATITSMRANDPRPNVMDIFAQTSSGAGTSGPLQPPPAHSEPVDIPSRPPSYNRAQSQYEPNLFPPGGVDALLSTSAPAGGSPLLLSNAATAPSSPVGESSKSLYDPQKGASRETVESMDLRSSTDLLGDAAVQPEDEGDDFDKLKAEREKEKLMRQQKNLARTISTAPGMEATAVPMVPMVPVNVAVQQAQLQPVASSADLLAGGDQFSNSTMKSEDAINGS

Target ORF information:

RefSeq Version	NM_001144561.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bestrophin 1, transcript variant B (Best1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144561.2

ORF Insert Sequence:

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGTTGGTAA TGCTAATGGT 
GATCCCGCCG CCGCTGCGGA TGCGAAAGCC CCGAACCCGG TTACGGGTCA AGTTCAAGTT 
CCGGGTGCGG ACACCGGCGT CCAAACCGTT ACTTATGATT TTCCCAAGGG CTCGCCAGAT 
GAGGAGGCCG ATGATGCCAG TGGCATTCAC TTCTCAGCTG GAAATGGCAA AATGCGCCTG 
GATTCCTCGC CCTCGCTGGT GAGCGTTTCG GGAACTCTAT CCCGGGTGAA TACGGTGGCC 
TCGGCCCTCA AACGTTTCCT GAGCCGCGAC GATAGCAGGC CGGGATCGGC AACGCCCAGT 
CAGGATCAGC CCTACAAATT CCCGGCCAGC GCCAGCTCGG CGAGTTTATC GGGTGCCGTG 
GTAGGATCGG CTACATCGGC CGGAAAACCA GCTGGCAGTC TTAGGATTAC GCAGCAAGTG 
ATCGAGGAGG TGGACGAACA GGCGACCATA ACATCCATGA GAGCCAATGA TCCACGTCCC 
AATGTCATGG ACATATTCGC ACAAACCTCG TCGGGAGCTG GAACCTCTGG ACCGCTGCAG 
CCACCACCGG CCCACTCGGA ACCGGTGGAC ATCCCGTCAC GTCCGCCCTC ATACAATCGG 
GCCCAATCCC AGTACGAACC CAACCTATTT CCACCTGGCG GAGTGGATGC ACTGCTCAGT 
ACTTCAGCTC CTGCGGGCGG AAGTCCCCTG CTCCTGTCTA ATGCAGCCAC TGCACCCAGT 
TCGCCAGTGG GCGAGAGCTC CAAGTCCCTA TACGATCCAC AAAAGGGCGC CAGCCGAGAG 
ACAGTGGAGA GCATGGACCT GAGGTCCTCC ACGGATCTAC TCGGCGATGC GGCAGTGCAG 
CCCGAAGACG AGGGCGATGA CTTCGATAAG CTGAAGGCGG AACGCGAGAA GGAGAAACTG 
ATGCGACAGC AAAAGAATCT GGCCAGAACT ATTAGCACCG CTCCGGGAAT GGAAGCCACG 
GCTGTGCCGA TGGTGCCAAT GGTCCCAGTG AACGTGGCAG TGCAACAGGC ACAGCTGCAA 
CCAGTTGCAT CCAGTGCCGA TCTTCTGGCC GGCGGAGATC AGTTCTCCAA TTCGACGATG 
AAATCGGAGG ACGCCATCAA CGGCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best1 ( NM_001275523.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262452.1 Drosophila melanogaster bestrophin 1, transcript variant D (Best1), mRNA.

Price & Availability↑

CloneID	OFa07011
Clone ID Related Accession (Same CDS sequence)	NM_001275523.1
Accession Version	NM_001275523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bestrophin 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGCCGATGA TGCCAGTGGC 
ATTCACTTCT CAGCTGGAAA TGGCAAAATG CGCCTGGATT CCTCGCCCTC GCTGGTGAGC 
GTTTCGGGAA CTCTATCCCG GGTGAATACG GTGGCCTCGG CCCTCAAACG TTTCCTGAGC 
CGCGACGATA GCAGGCCGGG ATCGGCAACG CCCAGTCAGG ATCAGCCCTA CAAATTCCCG 
GCCAGCGCCA GCTCGGCGAG TTTATCGGGT GCCGTGGTAG GATCGGCTAC ATCGGCCGGA 
AAACCAGCTG GCAGTCTTAG GATTACGCAG CAAGTGATCG AGGAGGTGGA CGAACAGGCG 
ACCATAACAT CCATGAGAGC CAATGATCCA CGTCCCAATG TCATGGACAT ATTCGCACAA 
ACCTCGTCGG GAGCTGGAAC CTCTGGACCG CTGCAGCCAC CACCGGCCCA CTCGGAACCG 
GTGGACATCC CGTCACGTCC GCCCTCATAC AATCGGGCCC AATCCCAGTA CGAACCCAAC 
CTATTTCCAC CTGGCGGAGT GGATGCACTG CTCAGTACTT CAGCTCCTGC GGGCGGAAGT 
CCCCTGCTCC TGTCTAATGC AGCCACTGCA CCCAGTTCGC CAGTGGGCGA GAGCTCCAAG 
TCCCTATACG ATCCACAAAA GGGCGCCAGC CGAGAGACAG AGAGGCCCGA TGCACCGCAG 
GTTTTCAGGT GGTTCGTCAC GCCCGTGGAG AGCATGGACC TGAGGTCCTC CACGGATCTA 
CTCGGCGATG CGGCAGTGCA GCCCGAAGAC GAGGGCGATG ACTTCGATAA GCTGAAGGCG 
GAACGCGAGA AGGAGAAACT GATGCGACAG CAAAAGAATC TGGCCAGAAC TATTAGCACC 
GCTCCGGGAA TGGAAGCCAC GGCTGTGCCG ATGGTGCCAA TGGTCCCAGT GAACGTGGCA 
GTGCAACAGG CACAGCTGCA ACCAGTTGCA TCCAGTGCCG ATCTTCTGGC CGGCGGAGAT 
CAGTTCTCCA ATTCGACGAT GAAATCGGAG GACGCCATCA ACGGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262452.1
CDS	439..2649
Translation	MTITYTGEVATCRGFGCFLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGLNPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDERGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDNEKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELLKDQYWDEVFPNELPYTIAAERFRENHPEPSTAKIEVPKNAAMPSTMSSVRIDEMADDASGIHFSAGNGKMRLDSSPSLVSVSGTLSRVNTVASALKRFLSRDDSRPGSATPSQDQPYKFPASASSASLSGAVVGSATSAGKPAGSLRITQQVIEEVDEQATITSMRANDPRPNVMDIFAQTSSGAGTSGPLQPPPAHSEPVDIPSRPPSYNRAQSQYEPNLFPPGGVDALLSTSAPAGGSPLLLSNAATAPSSPVGESSKSLYDPQKGASRETERPDAPQVFRWFVTPVESMDLRSSTDLLGDAAVQPEDEGDDFDKLKAEREKEKLMRQQKNLARTISTAPGMEATAVPMVPMVPVNVAVQQAQLQPVASSADLLAGGDQFSNSTMKSEDAINGS

Target ORF information:

RefSeq Version	NM_001275523.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bestrophin 1, transcript variant D (Best1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275523.1

ORF Insert Sequence:

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGCCGATGA TGCCAGTGGC 
ATTCACTTCT CAGCTGGAAA TGGCAAAATG CGCCTGGATT CCTCGCCCTC GCTGGTGAGC 
GTTTCGGGAA CTCTATCCCG GGTGAATACG GTGGCCTCGG CCCTCAAACG TTTCCTGAGC 
CGCGACGATA GCAGGCCGGG ATCGGCAACG CCCAGTCAGG ATCAGCCCTA CAAATTCCCG 
GCCAGCGCCA GCTCGGCGAG TTTATCGGGT GCCGTGGTAG GATCGGCTAC ATCGGCCGGA 
AAACCAGCTG GCAGTCTTAG GATTACGCAG CAAGTGATCG AGGAGGTGGA CGAACAGGCG 
ACCATAACAT CCATGAGAGC CAATGATCCA CGTCCCAATG TCATGGACAT ATTCGCACAA 
ACCTCGTCGG GAGCTGGAAC CTCTGGACCG CTGCAGCCAC CACCGGCCCA CTCGGAACCG 
GTGGACATCC CGTCACGTCC GCCCTCATAC AATCGGGCCC AATCCCAGTA CGAACCCAAC 
CTATTTCCAC CTGGCGGAGT GGATGCACTG CTCAGTACTT CAGCTCCTGC GGGCGGAAGT 
CCCCTGCTCC TGTCTAATGC AGCCACTGCA CCCAGTTCGC CAGTGGGCGA GAGCTCCAAG 
TCCCTATACG ATCCACAAAA GGGCGCCAGC CGAGAGACAG AGAGGCCCGA TGCACCGCAG 
GTTTTCAGGT GGTTCGTCAC GCCCGTGGAG AGCATGGACC TGAGGTCCTC CACGGATCTA 
CTCGGCGATG CGGCAGTGCA GCCCGAAGAC GAGGGCGATG ACTTCGATAA GCTGAAGGCG 
GAACGCGAGA AGGAGAAACT GATGCGACAG CAAAAGAATC TGGCCAGAAC TATTAGCACC 
GCTCCGGGAA TGGAAGCCAC GGCTGTGCCG ATGGTGCCAA TGGTCCCAGT GAACGTGGCA 
GTGCAACAGG CACAGCTGCA ACCAGTTGCA TCCAGTGCCG ATCTTCTGGC CGGCGGAGAT 
CAGTTCTCCA ATTCGACGAT GAAATCGGAG GACGCCATCA ACGGCAGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best1 ( NM_001260089.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247018.1 Drosophila melanogaster bestrophin 1, transcript variant C (Best1), mRNA.

Price & Availability↑

CloneID	OFa07012
Clone ID Related Accession (Same CDS sequence)	NM_001260089.2
Accession Version	NM_001260089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bestrophin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260089.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGTTGGTAA TGCTAATGGT 
GATCCCGCCG CCGCTGCGGA TGCGAAAGCC CCGAACCCGG TTACGGGTCA AGTTCAAGTT 
CCGGGTGCGG ACACCGGCGT CCAAACCGTT ACTTATGATT TTCCCAAGGG CTCGCCAGAT 
GAGGAGGCCG ATGATGCCAG TGGCATTCAC TTCTCAGCTG GAAATGGCAA AATGCGCCTG 
GATTCCTCGC CCTCGCTGGT GAGCGTTTCG GGAACTCTAT CCCGGGTGAA TACGGTGGCC 
TCGGCCCTCA AACGTTTCCT GAGCCGCGAC GATAGCAGGC CGGGATCGGC AACGCCCAGT 
CAGGATCAGC CCTACAAATT CCCGGCCAGC GCCAGCTCGG CGAGTTTATC GGGTGCCGTG 
GTAGGATCGG CTACATCGGC CGGAAAACCA GCTGGCAGTC TTAGGATTAC GCAGCAAGTG 
ATCGAGGAGG TGGACGAACA GGCGACCATA ACATCCATGA GAGCCAATGA TCCACGTCCC 
AATGTCATGG ACATATTCGC ACAAACCTCG TCGGGAGCTG GAACCTCTGG ACCGCTGCAG 
CCACCACCGG CCCACTCGGA ACCGGTGGAC ATCCCGTCAC GTCCGCCCTC ATACAATCGG 
GCCCAATCCC AGTACGAACC CAACCTATTT CCACCTGGCG GAGTGGATGC ACTGCTCAGT 
ACTTCAGCTC CTGCGGGCGG AAGTCCCCTG CTCCTGTCTA ATGCAGCCAC TGCACCCAGT 
TCGCCAGTGG GCGAGAGCTC CAAGTCCCTA TACGATCCAC AAAAGGGCGC CAGCCGAGAG 
ACAGAGAGGC CCGATGCACC GCAGGTTTTC AGGTGGTTCG TCACGCCCGT GGAGAGCATG 
GACCTGAGGT CCTCCACGGA TCTACTCGGC GATGCGGCAG TGCAGCCCGA AGACGAGGGC 
GATGACTTCG ATAAGCTGAA GGCGGAACGC GAGAAGGAGA AACTGATGCG ACAGCAAAAG 
AATCTGGCCA GAACTATTAG CACCGCTCCG GGAATGGAAG CCACGGCTGT GCCGATGGTG 
CCAATGGTCC CAGTGAACGT GGCAGTGCAA CAGGCACAGC TGCAACCAGT TGCATCCAGT 
GCCGATCTTC TGGCCGGCGG AGATCAGTTC TCCAATTCGA CGATGAAATC GGAGGACGCC 
ATCAACGGCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247018.1
CDS	439..2793
Translation	MTITYTGEVATCRGFGCFLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGLNPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDERGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDNEKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELLKDQYWDEVFPNELPYTIAAERFRENHPEPSTAKIEVPKNAAMPSTMSSVRIDEMVGNANGDPAAAADAKAPNPVTGQVQVPGADTGVQTVTYDFPKGSPDEEADDASGIHFSAGNGKMRLDSSPSLVSVSGTLSRVNTVASALKRFLSRDDSRPGSATPSQDQPYKFPASASSASLSGAVVGSATSAGKPAGSLRITQQVIEEVDEQATITSMRANDPRPNVMDIFAQTSSGAGTSGPLQPPPAHSEPVDIPSRPPSYNRAQSQYEPNLFPPGGVDALLSTSAPAGGSPLLLSNAATAPSSPVGESSKSLYDPQKGASRETERPDAPQVFRWFVTPVESMDLRSSTDLLGDAAVQPEDEGDDFDKLKAEREKEKLMRQQKNLARTISTAPGMEATAVPMVPMVPVNVAVQQAQLQPVASSADLLAGGDQFSNSTMKSEDAINGS

Target ORF information:

RefSeq Version	NM_001260089.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bestrophin 1, transcript variant C (Best1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260089.2

ORF Insert Sequence:

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGTTGGTAA TGCTAATGGT 
GATCCCGCCG CCGCTGCGGA TGCGAAAGCC CCGAACCCGG TTACGGGTCA AGTTCAAGTT 
CCGGGTGCGG ACACCGGCGT CCAAACCGTT ACTTATGATT TTCCCAAGGG CTCGCCAGAT 
GAGGAGGCCG ATGATGCCAG TGGCATTCAC TTCTCAGCTG GAAATGGCAA AATGCGCCTG 
GATTCCTCGC CCTCGCTGGT GAGCGTTTCG GGAACTCTAT CCCGGGTGAA TACGGTGGCC 
TCGGCCCTCA AACGTTTCCT GAGCCGCGAC GATAGCAGGC CGGGATCGGC AACGCCCAGT 
CAGGATCAGC CCTACAAATT CCCGGCCAGC GCCAGCTCGG CGAGTTTATC GGGTGCCGTG 
GTAGGATCGG CTACATCGGC CGGAAAACCA GCTGGCAGTC TTAGGATTAC GCAGCAAGTG 
ATCGAGGAGG TGGACGAACA GGCGACCATA ACATCCATGA GAGCCAATGA TCCACGTCCC 
AATGTCATGG ACATATTCGC ACAAACCTCG TCGGGAGCTG GAACCTCTGG ACCGCTGCAG 
CCACCACCGG CCCACTCGGA ACCGGTGGAC ATCCCGTCAC GTCCGCCCTC ATACAATCGG 
GCCCAATCCC AGTACGAACC CAACCTATTT CCACCTGGCG GAGTGGATGC ACTGCTCAGT 
ACTTCAGCTC CTGCGGGCGG AAGTCCCCTG CTCCTGTCTA ATGCAGCCAC TGCACCCAGT 
TCGCCAGTGG GCGAGAGCTC CAAGTCCCTA TACGATCCAC AAAAGGGCGC CAGCCGAGAG 
ACAGAGAGGC CCGATGCACC GCAGGTTTTC AGGTGGTTCG TCACGCCCGT GGAGAGCATG 
GACCTGAGGT CCTCCACGGA TCTACTCGGC GATGCGGCAG TGCAGCCCGA AGACGAGGGC 
GATGACTTCG ATAAGCTGAA GGCGGAACGC GAGAAGGAGA AACTGATGCG ACAGCAAAAG 
AATCTGGCCA GAACTATTAG CACCGCTCCG GGAATGGAAG CCACGGCTGT GCCGATGGTG 
CCAATGGTCC CAGTGAACGT GGCAGTGCAA CAGGCACAGC TGCAACCAGT TGCATCCAGT 
GCCGATCTTC TGGCCGGCGG AGATCAGTTC TCCAATTCGA CGATGAAATC GGAGGACGCC 
ATCAACGGCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Best1 ( NM_144346.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652603.1 Drosophila melanogaster bestrophin 1, transcript variant A (Best1), mRNA.

Price & Availability↑

CloneID	OFa08744
Clone ID Related Accession (Same CDS sequence)	NM_144346.4
Accession Version	NM_144346.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bestrophin 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_144346.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGCCGATGA TGCCAGTGGC 
ATTCACTTCT CAGCTGGAAA TGGCAAAATG CGCCTGGATT CCTCGCCCTC GCTGGTGAGC 
GTTTCGGGAA CTCTATCCCG GGTGAATACG GTGGCCTCGG CCCTCAAACG TTTCCTGAGC 
CGCGACGATA GCAGGCCGGG ATCGGCAACG CCCAGTCAGG ATCAGCCCTA CAAATTCCCG 
GCCAGCGCCA GCTCGGCGAG TTTATCGGGT GCCGTGGTAG GATCGGCTAC ATCGGCCGGA 
AAACCAGCTG GCAGTCTTAG GATTACGCAG CAAGTGATCG AGGAGGTGGA CGAACAGGCG 
ACCATAACAT CCATGAGAGC CAATGATCCA CGTCCCAATG TCATGGACAT ATTCGCACAA 
ACCTCGTCGG GAGCTGGAAC CTCTGGACCG CTGCAGCCAC CACCGGCCCA CTCGGAACCG 
GTGGACATCC CGTCACGTCC GCCCTCATAC AATCGGGCCC AATCCCAGTA CGAACCCAAC 
CTATTTCCAC CTGGCGGAGT GGATGCACTG CTCAGTACTT CAGCTCCTGC GGGCGGAAGT 
CCCCTGCTCC TGTCTAATGC AGCCACTGCA CCCAGTTCGC CAGTGGGCGA GAGCTCCAAG 
TCCCTATACG ATCCACAAAA GGGCGCCAGC CGAGAGACAG TGGAGAGCAT GGACCTGAGG 
TCCTCCACGG ATCTACTCGG CGATGCGGCA GTGCAGCCCG AAGACGAGGG CGATGACTTC 
GATAAGCTGA AGGCGGAACG CGAGAAGGAG AAACTGATGC GACAGCAAAA GAATCTGGCC 
AGAACTATTA GCACCGCTCC GGGAATGGAA GCCACGGCTG TGCCGATGGT GCCAATGGTC 
CCAGTGAACG TGGCAGTGCA ACAGGCACAG CTGCAACCAG TTGCATCCAG TGCCGATCTT 
CTGGCCGGCG GAGATCAGTT CTCCAATTCG ACGATGAAAT CGGAGGACGC CATCAACGGC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652603.1
CDS	439..2604
Translation	MTITYTGEVATCRGFGCFLKLLLRWRGSIYKLVWLDLLAFLTIYYAINMVYRFGLNPAQKETFEAIVQYCDSYRELIPLSFVLGFYVSIVMTRWWNQYTSIPWPDPIAVFVSSNVHGQDERGRMMRRTIMRYVCLCLTMVLANVSPRVKKRFPGLNNLVEAGLLNDNEKTIIETMNKAFPRPSKHWLPIVWAASIITRARKEGRIRDDFAVKTIIDELNKFRGQCGLLISYDTISVPLVYTQVVTLAVYSYFLTCCMGQQWTDGKVVGNTTYLNKVDLYFPVFTTLQFFFYMGWLKVAESLINPFGEDDDDFEVNWMVDRNLQVSYLIVDEMHHDHPELLKDQYWDEVFPNELPYTIAAERFRENHPEPSTAKIEVPKNAAMPSTMSSVRIDEMADDASGIHFSAGNGKMRLDSSPSLVSVSGTLSRVNTVASALKRFLSRDDSRPGSATPSQDQPYKFPASASSASLSGAVVGSATSAGKPAGSLRITQQVIEEVDEQATITSMRANDPRPNVMDIFAQTSSGAGTSGPLQPPPAHSEPVDIPSRPPSYNRAQSQYEPNLFPPGGVDALLSTSAPAGGSPLLLSNAATAPSSPVGESSKSLYDPQKGASRETVESMDLRSSTDLLGDAAVQPEDEGDDFDKLKAEREKEKLMRQQKNLARTISTAPGMEATAVPMVPMVPVNVAVQQAQLQPVASSADLLAGGDQFSNSTMKSEDAINGS

Target ORF information:

RefSeq Version	NM_144346.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bestrophin 1, transcript variant A (Best1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144346.4

ORF Insert Sequence:

ATGACAATTA CGTACACAGG TGAAGTGGCC ACTTGTCGCG GCTTTGGCTG TTTTCTCAAA 
TTGCTGCTCA GATGGCGAGG AAGCATTTAC AAACTGGTTT GGCTAGATCT TCTGGCCTTC 
TTGACCATTT ACTATGCGAT CAACATGGTG TATCGCTTTG GCCTCAACCC CGCACAAAAA 
GAAACCTTTG AGGCCATTGT TCAGTACTGT GATAGTTACA GAGAACTCAT ACCCCTGTCC 
TTCGTGCTTG GTTTCTATGT ATCGATTGTG ATGACCCGTT GGTGGAATCA GTACACCTCC 
ATTCCCTGGC CAGATCCCAT CGCCGTGTTT GTCAGCTCGA ATGTCCATGG CCAGGATGAG 
CGAGGACGCA TGATGAGGCG AACAATAATG CGATATGTGT GCCTTTGCCT GACGATGGTC 
CTGGCGAATG TTTCGCCGAG GGTGAAGAAG CGTTTCCCCG GCCTAAATAA TCTGGTGGAA 
GCGGGTCTGC TAAATGACAA TGAAAAGACC ATCATCGAGA CCATGAACAA GGCCTTTCCC 
AGACCTTCGA AGCACTGGCT GCCCATCGTT TGGGCTGCCA GTATTATAAC CAGGGCCAGA 
AAGGAAGGTC GCATTCGTGA TGATTTTGCT GTGAAGACCA TCATCGATGA GCTAAATAAG 
TTTCGTGGTC AGTGTGGACT CCTCATCAGC TACGATACCA TTAGTGTACC TCTGGTGTAC 
ACCCAAGTGG TGACCCTGGC GGTGTATTCG TACTTCCTTA CCTGCTGCAT GGGTCAACAA 
TGGACCGATG GCAAGGTGGT GGGCAATACC ACATACCTGA ACAAGGTGGA TCTATACTTT 
CCTGTATTTA CAACGCTGCA GTTCTTCTTC TACATGGGTT GGCTCAAGGT GGCCGAGTCG 
CTGATAAATC CATTTGGCGA AGACGATGAT GATTTTGAGG TCAACTGGAT GGTGGATCGC 
AATCTTCAGG TGTCCTATCT GATCGTCGAC GAGATGCACC ATGACCATCC GGAGCTGTTA 
AAGGATCAGT ACTGGGACGA GGTGTTCCCC AACGAGCTGC CCTACACAAT AGCTGCCGAA 
CGATTCCGGG AGAATCATCC AGAGCCGTCC ACTGCCAAGA TCGAGGTGCC CAAGAATGCG 
GCCATGCCAT CGACAATGTC GTCCGTTCGC ATCGATGAAA TGGCCGATGA TGCCAGTGGC 
ATTCACTTCT CAGCTGGAAA TGGCAAAATG CGCCTGGATT CCTCGCCCTC GCTGGTGAGC 
GTTTCGGGAA CTCTATCCCG GGTGAATACG GTGGCCTCGG CCCTCAAACG TTTCCTGAGC 
CGCGACGATA GCAGGCCGGG ATCGGCAACG CCCAGTCAGG ATCAGCCCTA CAAATTCCCG 
GCCAGCGCCA GCTCGGCGAG TTTATCGGGT GCCGTGGTAG GATCGGCTAC ATCGGCCGGA 
AAACCAGCTG GCAGTCTTAG GATTACGCAG CAAGTGATCG AGGAGGTGGA CGAACAGGCG 
ACCATAACAT CCATGAGAGC CAATGATCCA CGTCCCAATG TCATGGACAT ATTCGCACAA 
ACCTCGTCGG GAGCTGGAAC CTCTGGACCG CTGCAGCCAC CACCGGCCCA CTCGGAACCG 
GTGGACATCC CGTCACGTCC GCCCTCATAC AATCGGGCCC AATCCCAGTA CGAACCCAAC 
CTATTTCCAC CTGGCGGAGT GGATGCACTG CTCAGTACTT CAGCTCCTGC GGGCGGAAGT 
CCCCTGCTCC TGTCTAATGC AGCCACTGCA CCCAGTTCGC CAGTGGGCGA GAGCTCCAAG 
TCCCTATACG ATCCACAAAA GGGCGCCAGC CGAGAGACAG TGGAGAGCAT GGACCTGAGG 
TCCTCCACGG ATCTACTCGG CGATGCGGCA GTGCAGCCCG AAGACGAGGG CGATGACTTC 
GATAAGCTGA AGGCGGAACG CGAGAAGGAG AAACTGATGC GACAGCAAAA GAATCTGGCC 
AGAACTATTA GCACCGCTCC GGGAATGGAA GCCACGGCTG TGCCGATGGT GCCAATGGTC 
CCAGTGAACG TGGCAGTGCA ACAGGCACAG CTGCAACCAG TTGCATCCAG TGCCGATCTT 
CTGGCCGGCG GAGATCAGTT CTCCAATTCG ACGATGAAAT CGGAGGACGC CATCAACGGC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Best1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報