wds cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wds
Entrez Gene ID	53428
Full Name	will die slowly
Synonyms	CG17437,Dmel\CG17437,EG:BACR25B3.7,WD5,WDR5,WDS,Wds,l(1)16-94,l(1)3Ad,l(1)G0251,l(1)zw8,zw-8,zw8
General protein information	Preferred Names will die slowly Names CG17437 gene product from transcript CG17437-RB CG17437-PA CG17437-PB lethal(1)3Ad wds-PA wds-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A6-3A6\|1-1.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258574.2	NP_001245503.1	will die slowly, isoform B
NM_080245.5	NP_524984.1	will die slowly, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	wdr5	NP_998264.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002019	XP_321036.4
Caenorhabditis elegans (roundworm)	wdr-5.1	NP_497749.1
Xenopus tropicalis (tropical clawed frog)	wdr5	NP_001011411.1
Bos taurus (cattle)	WDR5	NP_001098945.1
Drosophila melanogaster (fruit fly)	wds	NP_001245503.1
Rattus norvegicus (Norway rat)	Wdr5	NP_001034123.1
Gallus gallus (chicken)	WDR5	NP_001006198.1
Homo sapiens (human)	WDR5	NP_438172.1
Pan troglodytes (chimpanzee)	WDR5	XP_001155196.2
	LOC722123	XP_001118316.2
Mus musculus (house mouse)	Wdr5	NP_543124.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012731	XP_307121.4
Canis lupus familiaris (dog)	WDR5	XP_005625215.1
Arabidopsis thaliana (thale cress)	AT3G49660	NP_190535.1
Oryza sativa (rice)	Os03g0725400	NP_001051130.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wds promotes Scm degradation through ubiquitination in vitro in Drosophila.
Title: A genetic screen in Drosophila implicates Sex comb on midleg (Scm) in tissue overgrowth and mechanisms of Scm degradation by Wds.

The essential Drosophila melanogaster gene wds (will die slowly) codes for a WD-repeat protein with seven repeats.
Title: The essential Drosophila melanogaster gene wds (will die slowly) codes for a WD-repeat protein with seven repeats.

The following wds gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wds cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02459	NM_001258574.2 Latest version!	Drosophila melanogaster will die slowly (wds), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02459	NM_080245.5 Latest version!	Drosophila melanogaster will die slowly (wds), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wds ( NM_001258574.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245503.1 Drosophila melanogaster will die slowly (wds), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02459
Clone ID Related Accession (Same CDS sequence)	NM_001258574.2 , NM_080245.5
Accession Version	NM_001258574.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	will die slowly, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258574.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCCCA TCGGAGCCGT GCACGGCGGC CATCCCGGCG TAGTGCATCC GCCACAGCAA 
CCACTGCCCA CGGCGCCCAG CGGCCCAAAC TCGCTGCAGC CGAACTCGGT GGGCCAGCCG 
GGGGCCACCA CCTCCTCGAA CAGCAGCGCC TCCAACAAGA GCTCGCTATC CGTCAAGCCC 
AACTACACGC TCAAGTTCAC GCTGGCCGGG CACACCAAGG CGGTGTCGGC GGTCAAGTTC 
AGTCCGAATG GCGAGTGGCT GGCCAGCTCC TCCGCTGATA AACTAATCAA AATCTGGGGA 
GCATACGATG GCAAGTTCGA GAAGACCATT TCGGGCCACA AGCTGGGCAT CAGCGATGTG 
GCCTGGAGCT CAGACTCGCG ACTCCTCGTG AGCGGCAGTG ATGACAAGAC GCTCAAGGTC 
TGGGAGCTGA GCACCGGGAA GAGCTTGAAA ACTCTGAAGG GCCACAGCAA CTATGTGTTC 
TGCTGCAACT TTAATCCGCA GTCCAATCTG ATCGTCTCCG GCAGCTTCGA CGAGAGCGTT 
CGCATATGGG ATGTGCGCAC CGGCAAGTGT CTGAAGACTC TACCCGCCCA TTCCGATCCC 
GTTTCGGCGG TACATTTCAA TCGCGACGGA TCGCTGATCG TGAGCAGCAG CTACGACGGC 
CTCTGTCGCA TATGGGACAC GGCCAGTGGA CAGTGCTTGA AAACCCTGAT CGACGACGAC 
AATCCGCCCG TCAGCTTTGT AAAGTTCTCG CCCAATGGCA AGTACATTTT GGCCGCCACG 
CTGGATAATA CGCTCAAGTT GTGGGACTAC TCGAAGGGCA AGTGCCTGAA GACGTATACG 
GGTCACAAGA ATGAGAAGTA CTGCATATTC GCCAACTTCT CGGTGACGGG AGGAAAGTGG 
ATCGTGAGTG GCAGCGAGGA CAACATGGTC TACATTTGGA ATCTGCAGAG CAAGGAGGTG 
GTGCAAAAGC TGCAGGGACA CACCGATACC GTTCTGTGCA CCGCCTGCCA TCCCACGGAG 
AACATCATTG CTTCCGCGGC GCTCGAGAAC GACAAGACCA TCAAGCTGTG GAAGTCGGAT 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245503.1
CDS	300..1385
Translation	MVPIGAVHGGHPGVVHPPQQPLPTAPSGPNSLQPNSVGQPGATTSSNSSASNKSSLSVKPNYTLKFTLAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSRLLVSGSDDKTLKVWELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQSKEVVQKLQGHTDTVLCTACHPTENIIASAALENDKTIKLWKSDT

Target ORF information:

RefSeq Version	NM_001258574.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster will die slowly (wds), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258574.2

ORF Insert Sequence:

ATGGTGCCCA TCGGAGCCGT GCACGGCGGC CATCCCGGCG TAGTGCATCC GCCACAGCAA 
CCACTGCCCA CGGCGCCCAG CGGCCCAAAC TCGCTGCAGC CGAACTCGGT GGGCCAGCCG 
GGGGCCACCA CCTCCTCGAA CAGCAGCGCC TCCAACAAGA GCTCGCTATC CGTCAAGCCC 
AACTACACGC TCAAGTTCAC GCTGGCCGGG CACACCAAGG CGGTGTCGGC GGTCAAGTTC 
AGTCCGAATG GCGAGTGGCT GGCCAGCTCC TCCGCTGATA AACTAATCAA AATCTGGGGA 
GCATACGATG GCAAGTTCGA GAAGACCATT TCGGGCCACA AGCTGGGCAT CAGCGATGTG 
GCCTGGAGCT CAGACTCGCG ACTCCTCGTG AGCGGCAGTG ATGACAAGAC GCTCAAGGTC 
TGGGAGCTGA GCACCGGGAA GAGCTTGAAA ACTCTGAAGG GCCACAGCAA CTATGTGTTC 
TGCTGCAACT TTAATCCGCA GTCCAATCTG ATCGTCTCCG GCAGCTTCGA CGAGAGCGTT 
CGCATATGGG ATGTGCGCAC CGGCAAGTGT CTGAAGACTC TACCCGCCCA TTCCGATCCC 
GTTTCGGCGG TACATTTCAA TCGCGACGGA TCGCTGATCG TGAGCAGCAG CTACGACGGC 
CTCTGTCGCA TATGGGACAC GGCCAGTGGA CAGTGCTTGA AAACCCTGAT CGACGACGAC 
AATCCGCCCG TCAGCTTTGT AAAGTTCTCG CCCAATGGCA AGTACATTTT GGCCGCCACG 
CTGGATAATA CGCTCAAGTT GTGGGACTAC TCGAAGGGCA AGTGCCTGAA GACGTATACG 
GGTCACAAGA ATGAGAAGTA CTGCATATTC GCCAACTTCT CGGTGACGGG AGGAAAGTGG 
ATCGTGAGTG GCAGCGAGGA CAACATGGTC TACATTTGGA ATCTGCAGAG CAAGGAGGTG 
GTGCAAAAGC TGCAGGGACA CACCGATACC GTTCTGTGCA CCGCCTGCCA TCCCACGGAG 
AACATCATTG CTTCCGCGGC GCTCGAGAAC GACAAGACCA TCAAGCTGTG GAAGTCGGAT 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wds ( NM_080245.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524984.1 Drosophila melanogaster will die slowly (wds), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02459
Clone ID Related Accession (Same CDS sequence)	NM_001258574.2 , NM_080245.5
Accession Version	NM_080245.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	will die slowly, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080245.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCCCA TCGGAGCCGT GCACGGCGGC CATCCCGGCG TAGTGCATCC GCCACAGCAA 
CCACTGCCCA CGGCGCCCAG CGGCCCAAAC TCGCTGCAGC CGAACTCGGT GGGCCAGCCG 
GGGGCCACCA CCTCCTCGAA CAGCAGCGCC TCCAACAAGA GCTCGCTATC CGTCAAGCCC 
AACTACACGC TCAAGTTCAC GCTGGCCGGG CACACCAAGG CGGTGTCGGC GGTCAAGTTC 
AGTCCGAATG GCGAGTGGCT GGCCAGCTCC TCCGCTGATA AACTAATCAA AATCTGGGGA 
GCATACGATG GCAAGTTCGA GAAGACCATT TCGGGCCACA AGCTGGGCAT CAGCGATGTG 
GCCTGGAGCT CAGACTCGCG ACTCCTCGTG AGCGGCAGTG ATGACAAGAC GCTCAAGGTC 
TGGGAGCTGA GCACCGGGAA GAGCTTGAAA ACTCTGAAGG GCCACAGCAA CTATGTGTTC 
TGCTGCAACT TTAATCCGCA GTCCAATCTG ATCGTCTCCG GCAGCTTCGA CGAGAGCGTT 
CGCATATGGG ATGTGCGCAC CGGCAAGTGT CTGAAGACTC TACCCGCCCA TTCCGATCCC 
GTTTCGGCGG TACATTTCAA TCGCGACGGA TCGCTGATCG TGAGCAGCAG CTACGACGGC 
CTCTGTCGCA TATGGGACAC GGCCAGTGGA CAGTGCTTGA AAACCCTGAT CGACGACGAC 
AATCCGCCCG TCAGCTTTGT AAAGTTCTCG CCCAATGGCA AGTACATTTT GGCCGCCACG 
CTGGATAATA CGCTCAAGTT GTGGGACTAC TCGAAGGGCA AGTGCCTGAA GACGTATACG 
GGTCACAAGA ATGAGAAGTA CTGCATATTC GCCAACTTCT CGGTGACGGG AGGAAAGTGG 
ATCGTGAGTG GCAGCGAGGA CAACATGGTC TACATTTGGA ATCTGCAGAG CAAGGAGGTG 
GTGCAAAAGC TGCAGGGACA CACCGATACC GTTCTGTGCA CCGCCTGCCA TCCCACGGAG 
AACATCATTG CTTCCGCGGC GCTCGAGAAC GACAAGACCA TCAAGCTGTG GAAGTCGGAT 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524984.1
CDS	300..1385
Translation	MVPIGAVHGGHPGVVHPPQQPLPTAPSGPNSLQPNSVGQPGATTSSNSSASNKSSLSVKPNYTLKFTLAGHTKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSRLLVSGSDDKTLKVWELSTGKSLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQSKEVVQKLQGHTDTVLCTACHPTENIIASAALENDKTIKLWKSDT

Target ORF information:

RefSeq Version	NM_080245.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster will die slowly (wds), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080245.5

ORF Insert Sequence:

ATGGTGCCCA TCGGAGCCGT GCACGGCGGC CATCCCGGCG TAGTGCATCC GCCACAGCAA 
CCACTGCCCA CGGCGCCCAG CGGCCCAAAC TCGCTGCAGC CGAACTCGGT GGGCCAGCCG 
GGGGCCACCA CCTCCTCGAA CAGCAGCGCC TCCAACAAGA GCTCGCTATC CGTCAAGCCC 
AACTACACGC TCAAGTTCAC GCTGGCCGGG CACACCAAGG CGGTGTCGGC GGTCAAGTTC 
AGTCCGAATG GCGAGTGGCT GGCCAGCTCC TCCGCTGATA AACTAATCAA AATCTGGGGA 
GCATACGATG GCAAGTTCGA GAAGACCATT TCGGGCCACA AGCTGGGCAT CAGCGATGTG 
GCCTGGAGCT CAGACTCGCG ACTCCTCGTG AGCGGCAGTG ATGACAAGAC GCTCAAGGTC 
TGGGAGCTGA GCACCGGGAA GAGCTTGAAA ACTCTGAAGG GCCACAGCAA CTATGTGTTC 
TGCTGCAACT TTAATCCGCA GTCCAATCTG ATCGTCTCCG GCAGCTTCGA CGAGAGCGTT 
CGCATATGGG ATGTGCGCAC CGGCAAGTGT CTGAAGACTC TACCCGCCCA TTCCGATCCC 
GTTTCGGCGG TACATTTCAA TCGCGACGGA TCGCTGATCG TGAGCAGCAG CTACGACGGC 
CTCTGTCGCA TATGGGACAC GGCCAGTGGA CAGTGCTTGA AAACCCTGAT CGACGACGAC 
AATCCGCCCG TCAGCTTTGT AAAGTTCTCG CCCAATGGCA AGTACATTTT GGCCGCCACG 
CTGGATAATA CGCTCAAGTT GTGGGACTAC TCGAAGGGCA AGTGCCTGAA GACGTATACG 
GGTCACAAGA ATGAGAAGTA CTGCATATTC GCCAACTTCT CGGTGACGGG AGGAAAGTGG 
ATCGTGAGTG GCAGCGAGGA CAACATGGTC TACATTTGGA ATCTGCAGAG CAAGGAGGTG 
GTGCAAAAGC TGCAGGGACA CACCGATACC GTTCTGTGCA CCGCCTGCCA TCCCACGGAG 
AACATCATTG CTTCCGCGGC GCTCGAGAAC GACAAGACCA TCAAGCTGTG GAAGTCGGAT 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wds cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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