Alk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alk
Entrez Gene ID	53425
Full Name	Anaplastic lymphoma kinase
Synonyms	ALK,CG8250,DAlk,DAlk53,Dmel\CG8250,alk,dALK,dAlk,mili
General protein information	Preferred Names Anaplastic lymphoma kinase Names CG8250 gene product from transcript CG8250-RB Alk-PA Alk-PB CG8250-PA CG8250-PB anaplastic lymphoma kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53C12-53C13

mRNA and Protein(s)

mRNA	Protein	Name
NM_144343.3	NP_652600.1	anaplastic lymphoma kinase, isoform A
NM_001274098.1	NP_001261027.1	anaplastic lymphoma kinase, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ltk	NP_001006661.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012070	XP_320455.4
Xenopus tropicalis (tropical clawed frog)	alk	XP_002933782.2
Drosophila melanogaster (fruit fly)	Alk	NP_001261027.1
Gallus gallus (chicken)	LTK	XP_004941681.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Opa protein is a novel regulator of embryonic Alk expression.
Title: The Zic family homologue Odd-paired regulates Alk expression in Drosophila.

mutations in Nf1 produce a sexually dimorphic short sleep phenotype, and suppress the long sleep phenotype of Alk. Thus Alk and Nf1 interact in both learning and sleep regulation, highlighting a common pathway in these two processes
Title: Anaplastic Lymphoma Kinase Acts in the Drosophila Mushroom Body to Negatively Regulate Sleep.

This study demonistrated that Signaling through the anaplastic lymphoma kinase directly regulates neuronal survival in visual pathway.
Title: Sequential axon-derived signals couple target survival and layer specificity in the Drosophila visual system.

Role of Lmd transcription factor in visceral mesoderm development in relationship to Jeb/Alk signal transduction. Alk signalling negatively regulates Lmd activity through the MAPK cascade resulting in a relocalisation of Lmd from nucleus to cytoplasm.
Title: Jeb/Alk signalling regulates the Lame duck GLI family transcription factor in the Drosophila visceral mesoderm.

These results identify dAlk as an upstream activator of dNf1-regulated Ras signaling responsible for several dNf1 defects, and they implicate human Alk as a potential therapeutic target in NF1
Title: The receptor tyrosine kinase Alk controls neurofibromin functions in Drosophila growth and learning.

The following Alk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11525	NM_001274098.1 Latest version!	Drosophila melanogaster anaplastic lymphoma kinase, transcript variant B (Alk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa11525	NM_144343.3 Latest version!	Drosophila melanogaster anaplastic lymphoma kinase, transcript variant A (Alk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alk ( NM_144343.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652600.1 Drosophila melanogaster anaplastic lymphoma kinase, transcript variant A (Alk), mRNA.

Price & Availability↑

CloneID	OFa11525
Clone ID Related Accession (Same CDS sequence)	NM_144343.3 , NM_001274098.1
Accession Version	NM_144343.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anaplastic lymphoma kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_144343.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACAGC TCAGGCAGCT GGTGGTGGTG CTGGCCATCC TATCCCTCCT TGTGGATCCC 
TCGTTCGCCC AGCGACCGCT GATCATGAAC CACACCTTCA GCTCCCTGCC GATGTCCCAG 
CATCCCAGTT TTAACAGCTG GCCAAGTGCA TCCGGGGGAA AGCAACTGCG CAATGGCAGG 
CTCCATGAGC AGCCTTTGCC CCTGCCACAA CCCACTTTGC CGGTGGCCAC ACAATACGAG 
GACTACGAGG CGGCACCGCC AGGGTCGCGA AGGGATAACA AGCGGCGACT GGCTCAGATG 
GCCCAACGCC CAGGCAGTGG GAACGGCGGG CGGCGAGGCA TGGAATCCCT TCGAAAGGAG 
CTTATGAATC CATCGGTTGG TGGTCCGGGC GGAGGTATTT CCGGCGGAGG ATCCGGCTAT 
CCTTCGTACT CCGCCACCTC TTCCATTGGA CGTATAGATG GCCTGGGTCA GGGCCTAGGA 
TCTGCGGATC GGTACGGAGC GTTATACGAC CAGCTGAGGC AGCCCAGTGG AATGGCAGCC 
ATGACAGCCG GTCCCCTTGG CGGACCCTAC TCGACAAGGT TCCCTCCTCT GTACGCCGGT 
GTAGACGACC AGCCCAAGCG CTCAAATCGA AAGAACTCCA TCAGCGAGCT CTATAAGCTG 
AAGAAGGCCC TCAACCAGGC AGATGAACCT GCTGGCGGGG TGACGCACGG AAACTTTGAA 
GGAGATCCAC AGCCCTCGTC GGCAGATCCA CTGCAAGAAA TCAAGGATCG TATACGAGAT 
ACATCACCAA ATGCGAACAC CTATGCTCCT CACACGGATG CCACACCATC ATCAGAGTAC 
GAGTACGAAC TTGGCGTGAA GTGCAACTTC GAGACCCCGT GCAGCTGGAC CTGGGGCAAC 
TATTCGGATG GTTTTCAAGT TATAACTGGA ACGGAATTGT CGAAAAGGAA TCTCACAGGA 
CTACTGCCAG GTCCTGCAGC CGATAGTATC GACGATGCGA ACGGTCACTT CCTGTACGCC 
CGCGTGAACC CCTCTTCAAG ACCCTTGAAT CTCACCTCAC CGGAATTTAG CACCACGATG 
GAGAAGTGCT TTTTAGAGGT CTACATGCAC CAGAGCGACA TGAGTCACGG ACTTTCGCGG 
GTGGTGGTTG AACTACTTCA CACGGCTGAG AGCAGTTGGG TTCCGGCCGA AATTCTGGGC 
GATAATGTGC GCCAATGGAC CAGGAAGGTA TATCGCCTGG GTCGCGTTTC CCGCGATTTC 
CGCATCGTTT TCGAGGTGGT TCCCGATCTG AGAGTGGGCC AGAAGGGTCA TGTGGCGTTG 
GACAACCTGC GCATGGTTAA CTGTTTTCCC GAGGGTACAA AGTCGGAGAA GTGCAGCACC 
TCGCAGGTGA AGTGCACCTC CAGCAAGGTG CCCGTGTGTA TCCATCTACC ACGAATCTGC 
GACATTACCA GGGACTGCGA TGAGGCGGAG GACGAGCAGC AGTCATGTGA TAAAATACCC 
TATGGCGGTC GTTGTGACTT CGAGGAGGAC TGGTGCGGTT GGCGGGACTC GGGAAAGACG 
ACGCTCACCT GGTCACGGCA CACGGGATCA TCTCCCACCC ACGATACGGG ACCGGATGGG 
GATCACACAA TGCAGCACCT GCAGAACAAC ACCAGTGGAT ACTATATGCT GGTCAATATG 
AATCAGCATA TGAACAACTC GGAAAAGAAT TCGATTATTG GATTTGCCAG CAATGCGATT 
ATGGTTAGCA AGACCTTCAA TCCGCCGCCA TCGGTACATG GAAACCCGGA CTCTCCCTAT 
CGTAACTCGT GTGTTGTGAG GTTTTTCATT CATCAATTCG GCAAGAATCC GGGCAGTATC 
AATCTCTCCG TGGTGGAGAT GAAGGAAAAG GAGAACATCA CCACTACACT GTGGTGGAGC 
ACCAAAAACC AGGGCAGCGA TTGGATGAGA GCGGAATATG TACTGCCTAA CATAACGTCC 
AAATACTATC TTCAGTTTGA AGCTCGCATG GGCATGAGAA TCTACTCAGA TGTGGCTGTG 
GATGACTTCT CACTGAGTCC CGAATGTTTT GGCCTCAATA TACCAGAAGA TCATCTAGGT 
GGCTACAACT ACTGGGATGT CCGACAGAAT CTAAAGAGTC CAACCTATAA AGACTTTGAA 
TATACCAACT ATCTTGAGTT AACCACCTGT GACACTCGTG GCATGATAGG ACCTTCCCAG 
GCGCAATGTG AAGCTGCCTA CAGGGAGCAA AATAAGACAC ATGTCCTGCG GGAAGTCCAT 
GTGGTGGAAG ATCAGAGCAG CTACAAGGGC ATGCAGAAGT GGAAGGTACC CCACGAAGGA 
CACTACACAA TCATTGCCAA AGGCGCCAGT GGAGGACTGG GTTCGGGTGG CGTAGGCAGC 
TCTCGAGGAT CCGTGGCTGT GGCCATTCTG GAACTTCACA AAAATGAGGA ACTGTATTTC 
CTCGTGGGAC AGCAGGGCGA GAACGCCTGC ATCAAGTCCA TGGGAGTTCT GAAGGAAGCT 
GGTTGCGGAA CCGATCACGA CTTGGACTTA GCCCAATATA GCTTCCGTTC CAAACAGGAT 
ATGGTCAAGA ATATCTATAT CGAAAATGGT GCCGGCGGCG GTGGTGGTGG CTCCTACGTG 
TTCCTTCTCA ATCAGGCCAA AAATGAGGCA GTCCCTCTTT TGGTAGCTGG CGGTGGAGGA 
GGTCTGGGAA TTGGCCAATA CATTGATGAG GACTTCCAGC ATGGTCAAAA GGCGAAGCCC 
CTTCAGGCTC CAGAAAGTGG ACAGATCAAT GGAGAGCCCT TGAATAAGAA GACAGCTGGT 
CCAGGTGGTG GATGGAGAGC CAAAGAGGAC CAGGCCCTGA GTCCCACCTA TGGAGCTGCC 
CTTCTCCAGG GCGGTCGAGG TGGACACTCC TGTTATGTGG AACTTGCGGA CAATGGAACT 
TCTGTTCACA GACATGGCCA AGGTGGATTT GGTGGTGGCG GTGGTGGCTG CAATACTGGA 
GGAGGCGGTG GCGGCTACGC TGGCGGAGAT GTCTACCTAA CCGAGTCGAA TGGCGAGGGC 
GGAAGTTCGT ACATCAGCCC CAGTCGAAGT CTTCGGGAAA TTAGTGAAAT ACATGCCGGC 
GCTAGCAGTG GACCAGGTGC CATCATCATT ATTCCGGCAA TCGAGGGCTG TGGATGCGAC 
TATAGGTGCG TGGCCTTGGA TGAGTTCCGA TCGAAGGTGC GGTGCATCTG CCCAGATGGC 
TGGAGCTTAA AACGGGACAA TCACACCGCC TGTGAGATCC GCGAGGAGGC GGGCAAGTCC 
TCCTTCCAGT ATCTGGTTTC CATCCTAATG ATCTCGCTTG CCGTGCTCTT CATCTGCATA 
GCTGCGCTCA TCTTTATGCT ATATAATCGC TATCAACGGA AGAAACAATC GAAGAAGCGC 
CACAAGATGC TCGTGGAGCA GGATCTTCAA CTTACCCGTT TGCGCAACAA TATCGACGAC 
TCCAATCTGA ATAACTTTAA CCCCAACTAT GGATGTGATG GTATTCTCAA TGGTCACATT 
GATGTCAATA GCTTGCCCCA GGTGGCACGC GATAGTCTGC AATTGGTCAA TGCTCTGGGC 
AAAGGTGCTT TTGGAGAGGT ATATATGGCA TTATATCGCC ATCGAGACGG AGATGCTGTG 
GAAATGGGCG TGGCAGTGAA AACCTTGCGG GAAGATCCCA AGCGGGAAAA GGAGGAGGAT 
TTCCTGAAGG AGGCGGCCAT AATGGCCAAG TTCAATCACC CGAATATGGT CCATTTAATA 
GGAGTCTGCT TTGACCGTCA GCCGTATTAT ATTGTCCTGG AACTTCTTGC TGGCGGGGAT 
CTACAGAAGT TTTTGCGCGA AAACAGGAAT ACTCCAGAGC GTCCCTCCCT CTTAACCATG 
AAGGATCTGC TTTTCTGCGC CCTCGACGTG GCCAAAGGAT GTCGCTACAT GGAGAGTAAA 
CGCTTCATCC ATCGGGATAT CGCCGCCAGG AACTGTCTGC TGAGCAGTAA GGGTCCTGGT 
CGCGTTGTGA AAATCGCCGA TTTTGGCATG TCCCGGGATA TCTACCGATC GGACTACTAT 
CGCAAGGGAG GCAAGGCGAT GCTACCCATC AAATGGATGC CGCCGGAAGC CTTCCTCGAT 
GGCATATTCA CCTCGAAAAC AGATGTCTGG TCCTTTGGCA TTCTCCTGTG GGAAGTCTTC 
AGTCTGGGCC GTTCACCATA TCCCGGCCAG CATAACACCC AGGTAATGGA GCTGGTTGTG 
CGTGGCGGTC GACTGGGATC GCCCACCGAG TGTCCCGTCT CCATATACAA GGTGATGGCC 
GACTGCTGGA ATCCCACGCC CGAGGATCGG CCCACATTTA TTACGTTGCT GGAGCATTTG 
ACCGCCTGCA CCCAGGATGC CAGCATAATG AACGCTCCTC TGCCCAATAT TCTGGGACCC 
ACAGCCTCCG AACGGGACGA CACAGTCATC CGGCCGCCCA ATGGCGAGGA GTTCTGTTTG 
GCGGTGCCGG ATTATCTGGT ACCTTTGCCA CCCGGCGGCA GCAACAATCC ATCGATGGCC 
AGTGGTTCGG GCTACGTGCC AGAATTGCAA CGCCAGCAGA TGAGCTCCTG CACTCCGCCG 
GCGGTCACAT CGCCCGCCGC ACCACATCCA AGGCCGGTGG AGAATATCGC ACCGACCAGC 
GGAGATTGCT GGGAGACGTC ATTTATACTA CCTAACTCGA AGGTGGAACC ACCAGTGGTT 
GGAAGTGGCC ACGATGTTCC ATTGGCCGGC GGTGAGGAGG CCAAACTGAT CAGTTTGGAC 
ACACCACAGC CCACACCAAC GACCATCCAA CCACCGCTGT CCTTCGCCTC GCAGCTTGAT 
GGCATCACAT TGGATCCCTC GGCACTGACC AAGAGTTTGC CCAACGGAAA TGCCAAGCAG 
TCGTATGCCA ATGTTCAGGT GAAAAACGAA GCCGAGGCCA AGGTCATCAA TGGTGTGGTT 
GGCAATGGAA CGGTGATGAA CGGGGACGTC TCGACGGGAT CGGGTGGTGC CGATTCACCT 
TTCACCATTC AGGGTTACGC GGAGCGATAC AAGGACAACA ACAATCATTC CGAAATCAGT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652600.1
CDS	579..5684
Translation	MPQLRQLVVVLAILSLLVDPSFAQRPLIMNHTFSSLPMSQHPSFNSWPSASGGKQLRNGRLHEQPLPLPQPTLPVATQYEDYEAAPPGSRRDNKRRLAQMAQRPGSGNGGRRGMESLRKELMNPSVGGPGGGISGGGSGYPSYSATSSIGRIDGLGQGLGSADRYGALYDQLRQPSGMAAMTAGPLGGPYSTRFPPLYAGVDDQPKRSNRKNSISELYKLKKALNQADEPAGGVTHGNFEGDPQPSSADPLQEIKDRIRDTSPNANTYAPHTDATPSSEYEYELGVKCNFETPCSWTWGNYSDGFQVITGTELSKRNLTGLLPGPAADSIDDANGHFLYARVNPSSRPLNLTSPEFSTTMEKCFLEVYMHQSDMSHGLSRVVVELLHTAESSWVPAEILGDNVRQWTRKVYRLGRVSRDFRIVFEVVPDLRVGQKGHVALDNLRMVNCFPEGTKSEKCSTSQVKCTSSKVPVCIHLPRICDITRDCDEAEDEQQSCDKIPYGGRCDFEEDWCGWRDSGKTTLTWSRHTGSSPTHDTGPDGDHTMQHLQNNTSGYYMLVNMNQHMNNSEKNSIIGFASNAIMVSKTFNPPPSVHGNPDSPYRNSCVVRFFIHQFGKNPGSINLSVVEMKEKENITTTLWWSTKNQGSDWMRAEYVLPNITSKYYLQFEARMGMRIYSDVAVDDFSLSPECFGLNIPEDHLGGYNYWDVRQNLKSPTYKDFEYTNYLELTTCDTRGMIGPSQAQCEAAYREQNKTHVLREVHVVEDQSSYKGMQKWKVPHEGHYTIIAKGASGGLGSGGVGSSRGSVAVAILELHKNEELYFLVGQQGENACIKSMGVLKEAGCGTDHDLDLAQYSFRSKQDMVKNIYIENGAGGGGGGSYVFLLNQAKNEAVPLLVAGGGGGLGIGQYIDEDFQHGQKAKPLQAPESGQINGEPLNKKTAGPGGGWRAKEDQALSPTYGAALLQGGRGGHSCYVELADNGTSVHRHGQGGFGGGGGGCNTGGGGGGYAGGDVYLTESNGEGGSSYISPSRSLREISEIHAGASSGPGAIIIIPAIEGCGCDYRCVALDEFRSKVRCICPDGWSLKRDNHTACEIREEAGKSSFQYLVSILMISLAVLFICIAALIFMLYNRYQRKKQSKKRHKMLVEQDLQLTRLRNNIDDSNLNNFNPNYGCDGILNGHIDVNSLPQVARDSLQLVNALGKGAFGEVYMALYRHRDGDAVEMGVAVKTLREDPKREKEEDFLKEAAIMAKFNHPNMVHLIGVCFDRQPYYIVLELLAGGDLQKFLRENRNTPERPSLLTMKDLLFCALDVAKGCRYMESKRFIHRDIAARNCLLSSKGPGRVVKIADFGMSRDIYRSDYYRKGGKAMLPIKWMPPEAFLDGIFTSKTDVWSFGILLWEVFSLGRSPYPGQHNTQVMELVVRGGRLGSPTECPVSIYKVMADCWNPTPEDRPTFITLLEHLTACTQDASIMNAPLPNILGPTASERDDTVIRPPNGEEFCLAVPDYLVPLPPGGSNNPSMASGSGYVPELQRQQMSSCTPPAVTSPAAPHPRPVENIAPTSGDCWETSFILPNSKVEPPVVGSGHDVPLAGGEEAKLISLDTPQPTPTTIQPPLSFASQLDGITLDPSALTKSLPNGNAKQSYANVQVKNEAEAKVINGVVGNGTVMNGDVSTGSGGADSPFTIQGYAERYKDNNNHSEISC

Target ORF information:

RefSeq Version	NM_144343.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anaplastic lymphoma kinase, transcript variant A (Alk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144343.3

ORF Insert Sequence:

ATGCCACAGC TCAGGCAGCT GGTGGTGGTG CTGGCCATCC TATCCCTCCT TGTGGATCCC 
TCGTTCGCCC AGCGACCGCT GATCATGAAC CACACCTTCA GCTCCCTGCC GATGTCCCAG 
CATCCCAGTT TTAACAGCTG GCCAAGTGCA TCCGGGGGAA AGCAACTGCG CAATGGCAGG 
CTCCATGAGC AGCCTTTGCC CCTGCCACAA CCCACTTTGC CGGTGGCCAC ACAATACGAG 
GACTACGAGG CGGCACCGCC AGGGTCGCGA AGGGATAACA AGCGGCGACT GGCTCAGATG 
GCCCAACGCC CAGGCAGTGG GAACGGCGGG CGGCGAGGCA TGGAATCCCT TCGAAAGGAG 
CTTATGAATC CATCGGTTGG TGGTCCGGGC GGAGGTATTT CCGGCGGAGG ATCCGGCTAT 
CCTTCGTACT CCGCCACCTC TTCCATTGGA CGTATAGATG GCCTGGGTCA GGGCCTAGGA 
TCTGCGGATC GGTACGGAGC GTTATACGAC CAGCTGAGGC AGCCCAGTGG AATGGCAGCC 
ATGACAGCCG GTCCCCTTGG CGGACCCTAC TCGACAAGGT TCCCTCCTCT GTACGCCGGT 
GTAGACGACC AGCCCAAGCG CTCAAATCGA AAGAACTCCA TCAGCGAGCT CTATAAGCTG 
AAGAAGGCCC TCAACCAGGC AGATGAACCT GCTGGCGGGG TGACGCACGG AAACTTTGAA 
GGAGATCCAC AGCCCTCGTC GGCAGATCCA CTGCAAGAAA TCAAGGATCG TATACGAGAT 
ACATCACCAA ATGCGAACAC CTATGCTCCT CACACGGATG CCACACCATC ATCAGAGTAC 
GAGTACGAAC TTGGCGTGAA GTGCAACTTC GAGACCCCGT GCAGCTGGAC CTGGGGCAAC 
TATTCGGATG GTTTTCAAGT TATAACTGGA ACGGAATTGT CGAAAAGGAA TCTCACAGGA 
CTACTGCCAG GTCCTGCAGC CGATAGTATC GACGATGCGA ACGGTCACTT CCTGTACGCC 
CGCGTGAACC CCTCTTCAAG ACCCTTGAAT CTCACCTCAC CGGAATTTAG CACCACGATG 
GAGAAGTGCT TTTTAGAGGT CTACATGCAC CAGAGCGACA TGAGTCACGG ACTTTCGCGG 
GTGGTGGTTG AACTACTTCA CACGGCTGAG AGCAGTTGGG TTCCGGCCGA AATTCTGGGC 
GATAATGTGC GCCAATGGAC CAGGAAGGTA TATCGCCTGG GTCGCGTTTC CCGCGATTTC 
CGCATCGTTT TCGAGGTGGT TCCCGATCTG AGAGTGGGCC AGAAGGGTCA TGTGGCGTTG 
GACAACCTGC GCATGGTTAA CTGTTTTCCC GAGGGTACAA AGTCGGAGAA GTGCAGCACC 
TCGCAGGTGA AGTGCACCTC CAGCAAGGTG CCCGTGTGTA TCCATCTACC ACGAATCTGC 
GACATTACCA GGGACTGCGA TGAGGCGGAG GACGAGCAGC AGTCATGTGA TAAAATACCC 
TATGGCGGTC GTTGTGACTT CGAGGAGGAC TGGTGCGGTT GGCGGGACTC GGGAAAGACG 
ACGCTCACCT GGTCACGGCA CACGGGATCA TCTCCCACCC ACGATACGGG ACCGGATGGG 
GATCACACAA TGCAGCACCT GCAGAACAAC ACCAGTGGAT ACTATATGCT GGTCAATATG 
AATCAGCATA TGAACAACTC GGAAAAGAAT TCGATTATTG GATTTGCCAG CAATGCGATT 
ATGGTTAGCA AGACCTTCAA TCCGCCGCCA TCGGTACATG GAAACCCGGA CTCTCCCTAT 
CGTAACTCGT GTGTTGTGAG GTTTTTCATT CATCAATTCG GCAAGAATCC GGGCAGTATC 
AATCTCTCCG TGGTGGAGAT GAAGGAAAAG GAGAACATCA CCACTACACT GTGGTGGAGC 
ACCAAAAACC AGGGCAGCGA TTGGATGAGA GCGGAATATG TACTGCCTAA CATAACGTCC 
AAATACTATC TTCAGTTTGA AGCTCGCATG GGCATGAGAA TCTACTCAGA TGTGGCTGTG 
GATGACTTCT CACTGAGTCC CGAATGTTTT GGCCTCAATA TACCAGAAGA TCATCTAGGT 
GGCTACAACT ACTGGGATGT CCGACAGAAT CTAAAGAGTC CAACCTATAA AGACTTTGAA 
TATACCAACT ATCTTGAGTT AACCACCTGT GACACTCGTG GCATGATAGG ACCTTCCCAG 
GCGCAATGTG AAGCTGCCTA CAGGGAGCAA AATAAGACAC ATGTCCTGCG GGAAGTCCAT 
GTGGTGGAAG ATCAGAGCAG CTACAAGGGC ATGCAGAAGT GGAAGGTACC CCACGAAGGA 
CACTACACAA TCATTGCCAA AGGCGCCAGT GGAGGACTGG GTTCGGGTGG CGTAGGCAGC 
TCTCGAGGAT CCGTGGCTGT GGCCATTCTG GAACTTCACA AAAATGAGGA ACTGTATTTC 
CTCGTGGGAC AGCAGGGCGA GAACGCCTGC ATCAAGTCCA TGGGAGTTCT GAAGGAAGCT 
GGTTGCGGAA CCGATCACGA CTTGGACTTA GCCCAATATA GCTTCCGTTC CAAACAGGAT 
ATGGTCAAGA ATATCTATAT CGAAAATGGT GCCGGCGGCG GTGGTGGTGG CTCCTACGTG 
TTCCTTCTCA ATCAGGCCAA AAATGAGGCA GTCCCTCTTT TGGTAGCTGG CGGTGGAGGA 
GGTCTGGGAA TTGGCCAATA CATTGATGAG GACTTCCAGC ATGGTCAAAA GGCGAAGCCC 
CTTCAGGCTC CAGAAAGTGG ACAGATCAAT GGAGAGCCCT TGAATAAGAA GACAGCTGGT 
CCAGGTGGTG GATGGAGAGC CAAAGAGGAC CAGGCCCTGA GTCCCACCTA TGGAGCTGCC 
CTTCTCCAGG GCGGTCGAGG TGGACACTCC TGTTATGTGG AACTTGCGGA CAATGGAACT 
TCTGTTCACA GACATGGCCA AGGTGGATTT GGTGGTGGCG GTGGTGGCTG CAATACTGGA 
GGAGGCGGTG GCGGCTACGC TGGCGGAGAT GTCTACCTAA CCGAGTCGAA TGGCGAGGGC 
GGAAGTTCGT ACATCAGCCC CAGTCGAAGT CTTCGGGAAA TTAGTGAAAT ACATGCCGGC 
GCTAGCAGTG GACCAGGTGC CATCATCATT ATTCCGGCAA TCGAGGGCTG TGGATGCGAC 
TATAGGTGCG TGGCCTTGGA TGAGTTCCGA TCGAAGGTGC GGTGCATCTG CCCAGATGGC 
TGGAGCTTAA AACGGGACAA TCACACCGCC TGTGAGATCC GCGAGGAGGC GGGCAAGTCC 
TCCTTCCAGT ATCTGGTTTC CATCCTAATG ATCTCGCTTG CCGTGCTCTT CATCTGCATA 
GCTGCGCTCA TCTTTATGCT ATATAATCGC TATCAACGGA AGAAACAATC GAAGAAGCGC 
CACAAGATGC TCGTGGAGCA GGATCTTCAA CTTACCCGTT TGCGCAACAA TATCGACGAC 
TCCAATCTGA ATAACTTTAA CCCCAACTAT GGATGTGATG GTATTCTCAA TGGTCACATT 
GATGTCAATA GCTTGCCCCA GGTGGCACGC GATAGTCTGC AATTGGTCAA TGCTCTGGGC 
AAAGGTGCTT TTGGAGAGGT ATATATGGCA TTATATCGCC ATCGAGACGG AGATGCTGTG 
GAAATGGGCG TGGCAGTGAA AACCTTGCGG GAAGATCCCA AGCGGGAAAA GGAGGAGGAT 
TTCCTGAAGG AGGCGGCCAT AATGGCCAAG TTCAATCACC CGAATATGGT CCATTTAATA 
GGAGTCTGCT TTGACCGTCA GCCGTATTAT ATTGTCCTGG AACTTCTTGC TGGCGGGGAT 
CTACAGAAGT TTTTGCGCGA AAACAGGAAT ACTCCAGAGC GTCCCTCCCT CTTAACCATG 
AAGGATCTGC TTTTCTGCGC CCTCGACGTG GCCAAAGGAT GTCGCTACAT GGAGAGTAAA 
CGCTTCATCC ATCGGGATAT CGCCGCCAGG AACTGTCTGC TGAGCAGTAA GGGTCCTGGT 
CGCGTTGTGA AAATCGCCGA TTTTGGCATG TCCCGGGATA TCTACCGATC GGACTACTAT 
CGCAAGGGAG GCAAGGCGAT GCTACCCATC AAATGGATGC CGCCGGAAGC CTTCCTCGAT 
GGCATATTCA CCTCGAAAAC AGATGTCTGG TCCTTTGGCA TTCTCCTGTG GGAAGTCTTC 
AGTCTGGGCC GTTCACCATA TCCCGGCCAG CATAACACCC AGGTAATGGA GCTGGTTGTG 
CGTGGCGGTC GACTGGGATC GCCCACCGAG TGTCCCGTCT CCATATACAA GGTGATGGCC 
GACTGCTGGA ATCCCACGCC CGAGGATCGG CCCACATTTA TTACGTTGCT GGAGCATTTG 
ACCGCCTGCA CCCAGGATGC CAGCATAATG AACGCTCCTC TGCCCAATAT TCTGGGACCC 
ACAGCCTCCG AACGGGACGA CACAGTCATC CGGCCGCCCA ATGGCGAGGA GTTCTGTTTG 
GCGGTGCCGG ATTATCTGGT ACCTTTGCCA CCCGGCGGCA GCAACAATCC ATCGATGGCC 
AGTGGTTCGG GCTACGTGCC AGAATTGCAA CGCCAGCAGA TGAGCTCCTG CACTCCGCCG 
GCGGTCACAT CGCCCGCCGC ACCACATCCA AGGCCGGTGG AGAATATCGC ACCGACCAGC 
GGAGATTGCT GGGAGACGTC ATTTATACTA CCTAACTCGA AGGTGGAACC ACCAGTGGTT 
GGAAGTGGCC ACGATGTTCC ATTGGCCGGC GGTGAGGAGG CCAAACTGAT CAGTTTGGAC 
ACACCACAGC CCACACCAAC GACCATCCAA CCACCGCTGT CCTTCGCCTC GCAGCTTGAT 
GGCATCACAT TGGATCCCTC GGCACTGACC AAGAGTTTGC CCAACGGAAA TGCCAAGCAG 
TCGTATGCCA ATGTTCAGGT GAAAAACGAA GCCGAGGCCA AGGTCATCAA TGGTGTGGTT 
GGCAATGGAA CGGTGATGAA CGGGGACGTC TCGACGGGAT CGGGTGGTGC CGATTCACCT 
TTCACCATTC AGGGTTACGC GGAGCGATAC AAGGACAACA ACAATCATTC CGAAATCAGT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alk ( NM_001274098.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261027.1 Drosophila melanogaster anaplastic lymphoma kinase, transcript variant B (Alk), mRNA.

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CloneID	OFa11525
Clone ID Related Accession (Same CDS sequence)	NM_144343.3 , NM_001274098.1
Accession Version	NM_001274098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	anaplastic lymphoma kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCACAGC TCAGGCAGCT GGTGGTGGTG CTGGCCATCC TATCCCTCCT TGTGGATCCC 
TCGTTCGCCC AGCGACCGCT GATCATGAAC CACACCTTCA GCTCCCTGCC GATGTCCCAG 
CATCCCAGTT TTAACAGCTG GCCAAGTGCA TCCGGGGGAA AGCAACTGCG CAATGGCAGG 
CTCCATGAGC AGCCTTTGCC CCTGCCACAA CCCACTTTGC CGGTGGCCAC ACAATACGAG 
GACTACGAGG CGGCACCGCC AGGGTCGCGA AGGGATAACA AGCGGCGACT GGCTCAGATG 
GCCCAACGCC CAGGCAGTGG GAACGGCGGG CGGCGAGGCA TGGAATCCCT TCGAAAGGAG 
CTTATGAATC CATCGGTTGG TGGTCCGGGC GGAGGTATTT CCGGCGGAGG ATCCGGCTAT 
CCTTCGTACT CCGCCACCTC TTCCATTGGA CGTATAGATG GCCTGGGTCA GGGCCTAGGA 
TCTGCGGATC GGTACGGAGC GTTATACGAC CAGCTGAGGC AGCCCAGTGG AATGGCAGCC 
ATGACAGCCG GTCCCCTTGG CGGACCCTAC TCGACAAGGT TCCCTCCTCT GTACGCCGGT 
GTAGACGACC AGCCCAAGCG CTCAAATCGA AAGAACTCCA TCAGCGAGCT CTATAAGCTG 
AAGAAGGCCC TCAACCAGGC AGATGAACCT GCTGGCGGGG TGACGCACGG AAACTTTGAA 
GGAGATCCAC AGCCCTCGTC GGCAGATCCA CTGCAAGAAA TCAAGGATCG TATACGAGAT 
ACATCACCAA ATGCGAACAC CTATGCTCCT CACACGGATG CCACACCATC ATCAGAGTAC 
GAGTACGAAC TTGGCGTGAA GTGCAACTTC GAGACCCCGT GCAGCTGGAC CTGGGGCAAC 
TATTCGGATG GTTTTCAAGT TATAACTGGA ACGGAATTGT CGAAAAGGAA TCTCACAGGA 
CTACTGCCAG GTCCTGCAGC CGATAGTATC GACGATGCGA ACGGTCACTT CCTGTACGCC 
CGCGTGAACC CCTCTTCAAG ACCCTTGAAT CTCACCTCAC CGGAATTTAG CACCACGATG 
GAGAAGTGCT TTTTAGAGGT CTACATGCAC CAGAGCGACA TGAGTCACGG ACTTTCGCGG 
GTGGTGGTTG AACTACTTCA CACGGCTGAG AGCAGTTGGG TTCCGGCCGA AATTCTGGGC 
GATAATGTGC GCCAATGGAC CAGGAAGGTA TATCGCCTGG GTCGCGTTTC CCGCGATTTC 
CGCATCGTTT TCGAGGTGGT TCCCGATCTG AGAGTGGGCC AGAAGGGTCA TGTGGCGTTG 
GACAACCTGC GCATGGTTAA CTGTTTTCCC GAGGGTACAA AGTCGGAGAA GTGCAGCACC 
TCGCAGGTGA AGTGCACCTC CAGCAAGGTG CCCGTGTGTA TCCATCTACC ACGAATCTGC 
GACATTACCA GGGACTGCGA TGAGGCGGAG GACGAGCAGC AGTCATGTGA TAAAATACCC 
TATGGCGGTC GTTGTGACTT CGAGGAGGAC TGGTGCGGTT GGCGGGACTC GGGAAAGACG 
ACGCTCACCT GGTCACGGCA CACGGGATCA TCTCCCACCC ACGATACGGG ACCGGATGGG 
GATCACACAA TGCAGCACCT GCAGAACAAC ACCAGTGGAT ACTATATGCT GGTCAATATG 
AATCAGCATA TGAACAACTC GGAAAAGAAT TCGATTATTG GATTTGCCAG CAATGCGATT 
ATGGTTAGCA AGACCTTCAA TCCGCCGCCA TCGGTACATG GAAACCCGGA CTCTCCCTAT 
CGTAACTCGT GTGTTGTGAG GTTTTTCATT CATCAATTCG GCAAGAATCC GGGCAGTATC 
AATCTCTCCG TGGTGGAGAT GAAGGAAAAG GAGAACATCA CCACTACACT GTGGTGGAGC 
ACCAAAAACC AGGGCAGCGA TTGGATGAGA GCGGAATATG TACTGCCTAA CATAACGTCC 
AAATACTATC TTCAGTTTGA AGCTCGCATG GGCATGAGAA TCTACTCAGA TGTGGCTGTG 
GATGACTTCT CACTGAGTCC CGAATGTTTT GGCCTCAATA TACCAGAAGA TCATCTAGGT 
GGCTACAACT ACTGGGATGT CCGACAGAAT CTAAAGAGTC CAACCTATAA AGACTTTGAA 
TATACCAACT ATCTTGAGTT AACCACCTGT GACACTCGTG GCATGATAGG ACCTTCCCAG 
GCGCAATGTG AAGCTGCCTA CAGGGAGCAA AATAAGACAC ATGTCCTGCG GGAAGTCCAT 
GTGGTGGAAG ATCAGAGCAG CTACAAGGGC ATGCAGAAGT GGAAGGTACC CCACGAAGGA 
CACTACACAA TCATTGCCAA AGGCGCCAGT GGAGGACTGG GTTCGGGTGG CGTAGGCAGC 
TCTCGAGGAT CCGTGGCTGT GGCCATTCTG GAACTTCACA AAAATGAGGA ACTGTATTTC 
CTCGTGGGAC AGCAGGGCGA GAACGCCTGC ATCAAGTCCA TGGGAGTTCT GAAGGAAGCT 
GGTTGCGGAA CCGATCACGA CTTGGACTTA GCCCAATATA GCTTCCGTTC CAAACAGGAT 
ATGGTCAAGA ATATCTATAT CGAAAATGGT GCCGGCGGCG GTGGTGGTGG CTCCTACGTG 
TTCCTTCTCA ATCAGGCCAA AAATGAGGCA GTCCCTCTTT TGGTAGCTGG CGGTGGAGGA 
GGTCTGGGAA TTGGCCAATA CATTGATGAG GACTTCCAGC ATGGTCAAAA GGCGAAGCCC 
CTTCAGGCTC CAGAAAGTGG ACAGATCAAT GGAGAGCCCT TGAATAAGAA GACAGCTGGT 
CCAGGTGGTG GATGGAGAGC CAAAGAGGAC CAGGCCCTGA GTCCCACCTA TGGAGCTGCC 
CTTCTCCAGG GCGGTCGAGG TGGACACTCC TGTTATGTGG AACTTGCGGA CAATGGAACT 
TCTGTTCACA GACATGGCCA AGGTGGATTT GGTGGTGGCG GTGGTGGCTG CAATACTGGA 
GGAGGCGGTG GCGGCTACGC TGGCGGAGAT GTCTACCTAA CCGAGTCGAA TGGCGAGGGC 
GGAAGTTCGT ACATCAGCCC CAGTCGAAGT CTTCGGGAAA TTAGTGAAAT ACATGCCGGC 
GCTAGCAGTG GACCAGGTGC CATCATCATT ATTCCGGCAA TCGAGGGCTG TGGATGCGAC 
TATAGGTGCG TGGCCTTGGA TGAGTTCCGA TCGAAGGTGC GGTGCATCTG CCCAGATGGC 
TGGAGCTTAA AACGGGACAA TCACACCGCC TGTGAGATCC GCGAGGAGGC GGGCAAGTCC 
TCCTTCCAGT ATCTGGTTTC CATCCTAATG ATCTCGCTTG CCGTGCTCTT CATCTGCATA 
GCTGCGCTCA TCTTTATGCT ATATAATCGC TATCAACGGA AGAAACAATC GAAGAAGCGC 
CACAAGATGC TCGTGGAGCA GGATCTTCAA CTTACCCGTT TGCGCAACAA TATCGACGAC 
TCCAATCTGA ATAACTTTAA CCCCAACTAT GGATGTGATG GTATTCTCAA TGGTCACATT 
GATGTCAATA GCTTGCCCCA GGTGGCACGC GATAGTCTGC AATTGGTCAA TGCTCTGGGC 
AAAGGTGCTT TTGGAGAGGT ATATATGGCA TTATATCGCC ATCGAGACGG AGATGCTGTG 
GAAATGGGCG TGGCAGTGAA AACCTTGCGG GAAGATCCCA AGCGGGAAAA GGAGGAGGAT 
TTCCTGAAGG AGGCGGCCAT AATGGCCAAG TTCAATCACC CGAATATGGT CCATTTAATA 
GGAGTCTGCT TTGACCGTCA GCCGTATTAT ATTGTCCTGG AACTTCTTGC TGGCGGGGAT 
CTACAGAAGT TTTTGCGCGA AAACAGGAAT ACTCCAGAGC GTCCCTCCCT CTTAACCATG 
AAGGATCTGC TTTTCTGCGC CCTCGACGTG GCCAAAGGAT GTCGCTACAT GGAGAGTAAA 
CGCTTCATCC ATCGGGATAT CGCCGCCAGG AACTGTCTGC TGAGCAGTAA GGGTCCTGGT 
CGCGTTGTGA AAATCGCCGA TTTTGGCATG TCCCGGGATA TCTACCGATC GGACTACTAT 
CGCAAGGGAG GCAAGGCGAT GCTACCCATC AAATGGATGC CGCCGGAAGC CTTCCTCGAT 
GGCATATTCA CCTCGAAAAC AGATGTCTGG TCCTTTGGCA TTCTCCTGTG GGAAGTCTTC 
AGTCTGGGCC GTTCACCATA TCCCGGCCAG CATAACACCC AGGTAATGGA GCTGGTTGTG 
CGTGGCGGTC GACTGGGATC GCCCACCGAG TGTCCCGTCT CCATATACAA GGTGATGGCC 
GACTGCTGGA ATCCCACGCC CGAGGATCGG CCCACATTTA TTACGTTGCT GGAGCATTTG 
ACCGCCTGCA CCCAGGATGC CAGCATAATG AACGCTCCTC TGCCCAATAT TCTGGGACCC 
ACAGCCTCCG AACGGGACGA CACAGTCATC CGGCCGCCCA ATGGCGAGGA GTTCTGTTTG 
GCGGTGCCGG ATTATCTGGT ACCTTTGCCA CCCGGCGGCA GCAACAATCC ATCGATGGCC 
AGTGGTTCGG GCTACGTGCC AGAATTGCAA CGCCAGCAGA TGAGCTCCTG CACTCCGCCG 
GCGGTCACAT CGCCCGCCGC ACCACATCCA AGGCCGGTGG AGAATATCGC ACCGACCAGC 
GGAGATTGCT GGGAGACGTC ATTTATACTA CCTAACTCGA AGGTGGAACC ACCAGTGGTT 
GGAAGTGGCC ACGATGTTCC ATTGGCCGGC GGTGAGGAGG CCAAACTGAT CAGTTTGGAC 
ACACCACAGC CCACACCAAC GACCATCCAA CCACCGCTGT CCTTCGCCTC GCAGCTTGAT 
GGCATCACAT TGGATCCCTC GGCACTGACC AAGAGTTTGC CCAACGGAAA TGCCAAGCAG 
TCGTATGCCA ATGTTCAGGT GAAAAACGAA GCCGAGGCCA AGGTCATCAA TGGTGTGGTT 
GGCAATGGAA CGGTGATGAA CGGGGACGTC TCGACGGGAT CGGGTGGTGC CGATTCACCT 
TTCACCATTC AGGGTTACGC GGAGCGATAC AAGGACAACA ACAATCATTC CGAAATCAGT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261027.1
CDS	517..5622
Translation	MPQLRQLVVVLAILSLLVDPSFAQRPLIMNHTFSSLPMSQHPSFNSWPSASGGKQLRNGRLHEQPLPLPQPTLPVATQYEDYEAAPPGSRRDNKRRLAQMAQRPGSGNGGRRGMESLRKELMNPSVGGPGGGISGGGSGYPSYSATSSIGRIDGLGQGLGSADRYGALYDQLRQPSGMAAMTAGPLGGPYSTRFPPLYAGVDDQPKRSNRKNSISELYKLKKALNQADEPAGGVTHGNFEGDPQPSSADPLQEIKDRIRDTSPNANTYAPHTDATPSSEYEYELGVKCNFETPCSWTWGNYSDGFQVITGTELSKRNLTGLLPGPAADSIDDANGHFLYARVNPSSRPLNLTSPEFSTTMEKCFLEVYMHQSDMSHGLSRVVVELLHTAESSWVPAEILGDNVRQWTRKVYRLGRVSRDFRIVFEVVPDLRVGQKGHVALDNLRMVNCFPEGTKSEKCSTSQVKCTSSKVPVCIHLPRICDITRDCDEAEDEQQSCDKIPYGGRCDFEEDWCGWRDSGKTTLTWSRHTGSSPTHDTGPDGDHTMQHLQNNTSGYYMLVNMNQHMNNSEKNSIIGFASNAIMVSKTFNPPPSVHGNPDSPYRNSCVVRFFIHQFGKNPGSINLSVVEMKEKENITTTLWWSTKNQGSDWMRAEYVLPNITSKYYLQFEARMGMRIYSDVAVDDFSLSPECFGLNIPEDHLGGYNYWDVRQNLKSPTYKDFEYTNYLELTTCDTRGMIGPSQAQCEAAYREQNKTHVLREVHVVEDQSSYKGMQKWKVPHEGHYTIIAKGASGGLGSGGVGSSRGSVAVAILELHKNEELYFLVGQQGENACIKSMGVLKEAGCGTDHDLDLAQYSFRSKQDMVKNIYIENGAGGGGGGSYVFLLNQAKNEAVPLLVAGGGGGLGIGQYIDEDFQHGQKAKPLQAPESGQINGEPLNKKTAGPGGGWRAKEDQALSPTYGAALLQGGRGGHSCYVELADNGTSVHRHGQGGFGGGGGGCNTGGGGGGYAGGDVYLTESNGEGGSSYISPSRSLREISEIHAGASSGPGAIIIIPAIEGCGCDYRCVALDEFRSKVRCICPDGWSLKRDNHTACEIREEAGKSSFQYLVSILMISLAVLFICIAALIFMLYNRYQRKKQSKKRHKMLVEQDLQLTRLRNNIDDSNLNNFNPNYGCDGILNGHIDVNSLPQVARDSLQLVNALGKGAFGEVYMALYRHRDGDAVEMGVAVKTLREDPKREKEEDFLKEAAIMAKFNHPNMVHLIGVCFDRQPYYIVLELLAGGDLQKFLRENRNTPERPSLLTMKDLLFCALDVAKGCRYMESKRFIHRDIAARNCLLSSKGPGRVVKIADFGMSRDIYRSDYYRKGGKAMLPIKWMPPEAFLDGIFTSKTDVWSFGILLWEVFSLGRSPYPGQHNTQVMELVVRGGRLGSPTECPVSIYKVMADCWNPTPEDRPTFITLLEHLTACTQDASIMNAPLPNILGPTASERDDTVIRPPNGEEFCLAVPDYLVPLPPGGSNNPSMASGSGYVPELQRQQMSSCTPPAVTSPAAPHPRPVENIAPTSGDCWETSFILPNSKVEPPVVGSGHDVPLAGGEEAKLISLDTPQPTPTTIQPPLSFASQLDGITLDPSALTKSLPNGNAKQSYANVQVKNEAEAKVINGVVGNGTVMNGDVSTGSGGADSPFTIQGYAERYKDNNNHSEISC

Target ORF information:

RefSeq Version	NM_001274098.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster anaplastic lymphoma kinase, transcript variant B (Alk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274098.1

ORF Insert Sequence:

ATGCCACAGC TCAGGCAGCT GGTGGTGGTG CTGGCCATCC TATCCCTCCT TGTGGATCCC 
TCGTTCGCCC AGCGACCGCT GATCATGAAC CACACCTTCA GCTCCCTGCC GATGTCCCAG 
CATCCCAGTT TTAACAGCTG GCCAAGTGCA TCCGGGGGAA AGCAACTGCG CAATGGCAGG 
CTCCATGAGC AGCCTTTGCC CCTGCCACAA CCCACTTTGC CGGTGGCCAC ACAATACGAG 
GACTACGAGG CGGCACCGCC AGGGTCGCGA AGGGATAACA AGCGGCGACT GGCTCAGATG 
GCCCAACGCC CAGGCAGTGG GAACGGCGGG CGGCGAGGCA TGGAATCCCT TCGAAAGGAG 
CTTATGAATC CATCGGTTGG TGGTCCGGGC GGAGGTATTT CCGGCGGAGG ATCCGGCTAT 
CCTTCGTACT CCGCCACCTC TTCCATTGGA CGTATAGATG GCCTGGGTCA GGGCCTAGGA 
TCTGCGGATC GGTACGGAGC GTTATACGAC CAGCTGAGGC AGCCCAGTGG AATGGCAGCC 
ATGACAGCCG GTCCCCTTGG CGGACCCTAC TCGACAAGGT TCCCTCCTCT GTACGCCGGT 
GTAGACGACC AGCCCAAGCG CTCAAATCGA AAGAACTCCA TCAGCGAGCT CTATAAGCTG 
AAGAAGGCCC TCAACCAGGC AGATGAACCT GCTGGCGGGG TGACGCACGG AAACTTTGAA 
GGAGATCCAC AGCCCTCGTC GGCAGATCCA CTGCAAGAAA TCAAGGATCG TATACGAGAT 
ACATCACCAA ATGCGAACAC CTATGCTCCT CACACGGATG CCACACCATC ATCAGAGTAC 
GAGTACGAAC TTGGCGTGAA GTGCAACTTC GAGACCCCGT GCAGCTGGAC CTGGGGCAAC 
TATTCGGATG GTTTTCAAGT TATAACTGGA ACGGAATTGT CGAAAAGGAA TCTCACAGGA 
CTACTGCCAG GTCCTGCAGC CGATAGTATC GACGATGCGA ACGGTCACTT CCTGTACGCC 
CGCGTGAACC CCTCTTCAAG ACCCTTGAAT CTCACCTCAC CGGAATTTAG CACCACGATG 
GAGAAGTGCT TTTTAGAGGT CTACATGCAC CAGAGCGACA TGAGTCACGG ACTTTCGCGG 
GTGGTGGTTG AACTACTTCA CACGGCTGAG AGCAGTTGGG TTCCGGCCGA AATTCTGGGC 
GATAATGTGC GCCAATGGAC CAGGAAGGTA TATCGCCTGG GTCGCGTTTC CCGCGATTTC 
CGCATCGTTT TCGAGGTGGT TCCCGATCTG AGAGTGGGCC AGAAGGGTCA TGTGGCGTTG 
GACAACCTGC GCATGGTTAA CTGTTTTCCC GAGGGTACAA AGTCGGAGAA GTGCAGCACC 
TCGCAGGTGA AGTGCACCTC CAGCAAGGTG CCCGTGTGTA TCCATCTACC ACGAATCTGC 
GACATTACCA GGGACTGCGA TGAGGCGGAG GACGAGCAGC AGTCATGTGA TAAAATACCC 
TATGGCGGTC GTTGTGACTT CGAGGAGGAC TGGTGCGGTT GGCGGGACTC GGGAAAGACG 
ACGCTCACCT GGTCACGGCA CACGGGATCA TCTCCCACCC ACGATACGGG ACCGGATGGG 
GATCACACAA TGCAGCACCT GCAGAACAAC ACCAGTGGAT ACTATATGCT GGTCAATATG 
AATCAGCATA TGAACAACTC GGAAAAGAAT TCGATTATTG GATTTGCCAG CAATGCGATT 
ATGGTTAGCA AGACCTTCAA TCCGCCGCCA TCGGTACATG GAAACCCGGA CTCTCCCTAT 
CGTAACTCGT GTGTTGTGAG GTTTTTCATT CATCAATTCG GCAAGAATCC GGGCAGTATC 
AATCTCTCCG TGGTGGAGAT GAAGGAAAAG GAGAACATCA CCACTACACT GTGGTGGAGC 
ACCAAAAACC AGGGCAGCGA TTGGATGAGA GCGGAATATG TACTGCCTAA CATAACGTCC 
AAATACTATC TTCAGTTTGA AGCTCGCATG GGCATGAGAA TCTACTCAGA TGTGGCTGTG 
GATGACTTCT CACTGAGTCC CGAATGTTTT GGCCTCAATA TACCAGAAGA TCATCTAGGT 
GGCTACAACT ACTGGGATGT CCGACAGAAT CTAAAGAGTC CAACCTATAA AGACTTTGAA 
TATACCAACT ATCTTGAGTT AACCACCTGT GACACTCGTG GCATGATAGG ACCTTCCCAG 
GCGCAATGTG AAGCTGCCTA CAGGGAGCAA AATAAGACAC ATGTCCTGCG GGAAGTCCAT 
GTGGTGGAAG ATCAGAGCAG CTACAAGGGC ATGCAGAAGT GGAAGGTACC CCACGAAGGA 
CACTACACAA TCATTGCCAA AGGCGCCAGT GGAGGACTGG GTTCGGGTGG CGTAGGCAGC 
TCTCGAGGAT CCGTGGCTGT GGCCATTCTG GAACTTCACA AAAATGAGGA ACTGTATTTC 
CTCGTGGGAC AGCAGGGCGA GAACGCCTGC ATCAAGTCCA TGGGAGTTCT GAAGGAAGCT 
GGTTGCGGAA CCGATCACGA CTTGGACTTA GCCCAATATA GCTTCCGTTC CAAACAGGAT 
ATGGTCAAGA ATATCTATAT CGAAAATGGT GCCGGCGGCG GTGGTGGTGG CTCCTACGTG 
TTCCTTCTCA ATCAGGCCAA AAATGAGGCA GTCCCTCTTT TGGTAGCTGG CGGTGGAGGA 
GGTCTGGGAA TTGGCCAATA CATTGATGAG GACTTCCAGC ATGGTCAAAA GGCGAAGCCC 
CTTCAGGCTC CAGAAAGTGG ACAGATCAAT GGAGAGCCCT TGAATAAGAA GACAGCTGGT 
CCAGGTGGTG GATGGAGAGC CAAAGAGGAC CAGGCCCTGA GTCCCACCTA TGGAGCTGCC 
CTTCTCCAGG GCGGTCGAGG TGGACACTCC TGTTATGTGG AACTTGCGGA CAATGGAACT 
TCTGTTCACA GACATGGCCA AGGTGGATTT GGTGGTGGCG GTGGTGGCTG CAATACTGGA 
GGAGGCGGTG GCGGCTACGC TGGCGGAGAT GTCTACCTAA CCGAGTCGAA TGGCGAGGGC 
GGAAGTTCGT ACATCAGCCC CAGTCGAAGT CTTCGGGAAA TTAGTGAAAT ACATGCCGGC 
GCTAGCAGTG GACCAGGTGC CATCATCATT ATTCCGGCAA TCGAGGGCTG TGGATGCGAC 
TATAGGTGCG TGGCCTTGGA TGAGTTCCGA TCGAAGGTGC GGTGCATCTG CCCAGATGGC 
TGGAGCTTAA AACGGGACAA TCACACCGCC TGTGAGATCC GCGAGGAGGC GGGCAAGTCC 
TCCTTCCAGT ATCTGGTTTC CATCCTAATG ATCTCGCTTG CCGTGCTCTT CATCTGCATA 
GCTGCGCTCA TCTTTATGCT ATATAATCGC TATCAACGGA AGAAACAATC GAAGAAGCGC 
CACAAGATGC TCGTGGAGCA GGATCTTCAA CTTACCCGTT TGCGCAACAA TATCGACGAC 
TCCAATCTGA ATAACTTTAA CCCCAACTAT GGATGTGATG GTATTCTCAA TGGTCACATT 
GATGTCAATA GCTTGCCCCA GGTGGCACGC GATAGTCTGC AATTGGTCAA TGCTCTGGGC 
AAAGGTGCTT TTGGAGAGGT ATATATGGCA TTATATCGCC ATCGAGACGG AGATGCTGTG 
GAAATGGGCG TGGCAGTGAA AACCTTGCGG GAAGATCCCA AGCGGGAAAA GGAGGAGGAT 
TTCCTGAAGG AGGCGGCCAT AATGGCCAAG TTCAATCACC CGAATATGGT CCATTTAATA 
GGAGTCTGCT TTGACCGTCA GCCGTATTAT ATTGTCCTGG AACTTCTTGC TGGCGGGGAT 
CTACAGAAGT TTTTGCGCGA AAACAGGAAT ACTCCAGAGC GTCCCTCCCT CTTAACCATG 
AAGGATCTGC TTTTCTGCGC CCTCGACGTG GCCAAAGGAT GTCGCTACAT GGAGAGTAAA 
CGCTTCATCC ATCGGGATAT CGCCGCCAGG AACTGTCTGC TGAGCAGTAA GGGTCCTGGT 
CGCGTTGTGA AAATCGCCGA TTTTGGCATG TCCCGGGATA TCTACCGATC GGACTACTAT 
CGCAAGGGAG GCAAGGCGAT GCTACCCATC AAATGGATGC CGCCGGAAGC CTTCCTCGAT 
GGCATATTCA CCTCGAAAAC AGATGTCTGG TCCTTTGGCA TTCTCCTGTG GGAAGTCTTC 
AGTCTGGGCC GTTCACCATA TCCCGGCCAG CATAACACCC AGGTAATGGA GCTGGTTGTG 
CGTGGCGGTC GACTGGGATC GCCCACCGAG TGTCCCGTCT CCATATACAA GGTGATGGCC 
GACTGCTGGA ATCCCACGCC CGAGGATCGG CCCACATTTA TTACGTTGCT GGAGCATTTG 
ACCGCCTGCA CCCAGGATGC CAGCATAATG AACGCTCCTC TGCCCAATAT TCTGGGACCC 
ACAGCCTCCG AACGGGACGA CACAGTCATC CGGCCGCCCA ATGGCGAGGA GTTCTGTTTG 
GCGGTGCCGG ATTATCTGGT ACCTTTGCCA CCCGGCGGCA GCAACAATCC ATCGATGGCC 
AGTGGTTCGG GCTACGTGCC AGAATTGCAA CGCCAGCAGA TGAGCTCCTG CACTCCGCCG 
GCGGTCACAT CGCCCGCCGC ACCACATCCA AGGCCGGTGG AGAATATCGC ACCGACCAGC 
GGAGATTGCT GGGAGACGTC ATTTATACTA CCTAACTCGA AGGTGGAACC ACCAGTGGTT 
GGAAGTGGCC ACGATGTTCC ATTGGCCGGC GGTGAGGAGG CCAAACTGAT CAGTTTGGAC 
ACACCACAGC CCACACCAAC GACCATCCAA CCACCGCTGT CCTTCGCCTC GCAGCTTGAT 
GGCATCACAT TGGATCCCTC GGCACTGACC AAGAGTTTGC CCAACGGAAA TGCCAAGCAG 
TCGTATGCCA ATGTTCAGGT GAAAAACGAA GCCGAGGCCA AGGTCATCAA TGGTGTGGTT 
GGCAATGGAA CGGTGATGAA CGGGGACGTC TCGACGGGAT CGGGTGGTGC CGATTCACCT 
TTCACCATTC AGGGTTACGC GGAGCGATAC AAGGACAACA ACAATCATTC CGAAATCAGT 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Alk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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