hrg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hrg
Entrez Gene ID	49636
Full Name	hiiragi
Synonyms	CG9854,Dmel\CG9854,PAP,Pap,l(2)07619,l(2)k07619
General protein information	Preferred Names hiiragi Names CG9854 gene product from transcript CG9854-RA CG9854-PA CG9854-PB CG9854-PC CG9854-PD Poly(A) polymerase hrg-PA hrg-PB hrg-PC hrg-PD poly (A) polymerase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 56E1-56E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_080529.4	NP_536790.1	hiiragi, isoform A
NM_001043097.3	NP_001036562.1	hiiragi, isoform C
NM_166372.3	NP_725919.1	hiiragi, isoform B
NM_001274168.2	NP_001261097.1	hiiragi, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	PAPOLG	NP_075045.2
Rattus norvegicus (Norway rat)	Papolg	NP_001100714.1
Gallus gallus (chicken)	PAPOLG	XP_003640929.2
Drosophila melanogaster (fruit fly)	hrg	NP_001036562.1
Eremothecium gossypii	AGOS_ABL002C	NP_982945.1
Xenopus tropicalis (tropical clawed frog)	papolg	NP_001005684.1
	PAPOLG	XP_001115228.1
Mus musculus (house mouse)	Papolg	NP_766143.2
Saccharomyces cerevisiae (baker's yeast)	PAP1	NP_012927.3
Kluyveromyces lactis	KLLA0B13255g	XP_452122.1
Oryza sativa (rice)	Os06g0319600	NP_001057507.2
Pan troglodytes (chimpanzee)	PAPOLG	XP_515496.3
Danio rerio (zebrafish)	papolg	NP_956915.1
Arabidopsis thaliana (thale cress)	PAPS1	NP_173240.2
Bos taurus (cattle)	PAPOLG	NP_001091535.1
Schizosaccharomyces pombe (fission yeast)	pla1	NP_595362.1
Neurospora crassa	NCU08983	XP_959011.2
Canis lupus familiaris (dog)	PAPOLG	XP_852387.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013207	XP_003436462.1
Magnaporthe oryzae (rice blast fungus)	MGG_06313	XP_003717272.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hrg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hrg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11471	NM_080529.4 Latest version!	Drosophila melanogaster hiiragi (hrg), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11471	NM_166372.3 Latest version!	Drosophila melanogaster hiiragi (hrg), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11471	NM_001043097.3 Latest version!	Drosophila melanogaster hiiragi (hrg), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12857	NM_001274168.2 Latest version!	Drosophila melanogaster hiiragi (hrg), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hrg ( NM_080529.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_536790.1 Drosophila melanogaster hiiragi (hrg), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11471
Clone ID Related Accession (Same CDS sequence)	NM_080529.4 , NM_001043097.3 , NM_166372.3
Accession Version	NM_080529.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hiiragi, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080529.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536790.1
CDS	750..2729
Translation	MWNSEPTHRQHHQHNGNSTSGGPPAKQLGMTSAISLAEPRPEDLQRTDELRGSLEPYNVFESQDELNHRMEILAKLNTLVKQWVKEISVSKNMPESAAEKLGGKIYTFGSYRLGVHHKGADIDALCVAPRNIERTDYFQSFFEVLKKQPEVTECRSVEEAFVPVIKMNFDGIEIDLLFARLSLKEIPDDFDLRDDNLLRNLDHRSVRSLNGCRVTDEILALVPNIENFRLALRTIKLWAKKHGIYSNSLGYFGGVTWAMLVARTCQLYPNAAAATLVHKFFLVFSRWKWPNPVLLKHPDNVNLRFQVWDPRVNASDRYHLMPIITPAYPQQNSTFNVSESTKKVILTEFNRGMNITDEIMLGRIPWERLFEAPSFFYRYRHFIVLLVNSQTADDHLEWCGLVESKVRLLIGNLERNPHIALAHVNPKCFEFKKGQSANNSQNNSGNEDDLKQSQGNQSAVTSAPFCSMWFIGLEFERSENLNVDLTESIQNFTEHVMMHGVNIKMLKEGMTIDARHVKRKQLSLYLDSDFLKRERKSMESHNNFNNTLLANRKRLSTELAQSQDPLPPGQQPSSGNRGRDSGAKIQRLSDSLTEENSNASSDMGAGTPTTPTTAQLSAPSFKSSGKNGSEIDVVEQEPTQPHNNGNASSNTTTTEVACS

Target ORF information:

RefSeq Version	NM_080529.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hiiragi (hrg), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080529.4

ORF Insert Sequence:

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hrg ( NM_001043097.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036562.1 Drosophila melanogaster hiiragi (hrg), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11471
Clone ID Related Accession (Same CDS sequence)	NM_080529.4 , NM_001043097.3 , NM_166372.3
Accession Version	NM_001043097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hiiragi, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001043097.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036562.1
CDS	750..2729
Translation	MWNSEPTHRQHHQHNGNSTSGGPPAKQLGMTSAISLAEPRPEDLQRTDELRGSLEPYNVFESQDELNHRMEILAKLNTLVKQWVKEISVSKNMPESAAEKLGGKIYTFGSYRLGVHHKGADIDALCVAPRNIERTDYFQSFFEVLKKQPEVTECRSVEEAFVPVIKMNFDGIEIDLLFARLSLKEIPDDFDLRDDNLLRNLDHRSVRSLNGCRVTDEILALVPNIENFRLALRTIKLWAKKHGIYSNSLGYFGGVTWAMLVARTCQLYPNAAAATLVHKFFLVFSRWKWPNPVLLKHPDNVNLRFQVWDPRVNASDRYHLMPIITPAYPQQNSTFNVSESTKKVILTEFNRGMNITDEIMLGRIPWERLFEAPSFFYRYRHFIVLLVNSQTADDHLEWCGLVESKVRLLIGNLERNPHIALAHVNPKCFEFKKGQSANNSQNNSGNEDDLKQSQGNQSAVTSAPFCSMWFIGLEFERSENLNVDLTESIQNFTEHVMMHGVNIKMLKEGMTIDARHVKRKQLSLYLDSDFLKRERKSMESHNNFNNTLLANRKRLSTELAQSQDPLPPGQQPSSGNRGRDSGAKIQRLSDSLTEENSNASSDMGAGTPTTPTTAQLSAPSFKSSGKNGSEIDVVEQEPTQPHNNGNASSNTTTTEVACS

Target ORF information:

RefSeq Version	NM_001043097.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hiiragi (hrg), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043097.3

ORF Insert Sequence:

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hrg ( NM_166372.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725919.1 Drosophila melanogaster hiiragi (hrg), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11471
Clone ID Related Accession (Same CDS sequence)	NM_080529.4 , NM_001043097.3 , NM_166372.3
Accession Version	NM_166372.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hiiragi, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166372.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725919.1
CDS	443..2422
Translation	MWNSEPTHRQHHQHNGNSTSGGPPAKQLGMTSAISLAEPRPEDLQRTDELRGSLEPYNVFESQDELNHRMEILAKLNTLVKQWVKEISVSKNMPESAAEKLGGKIYTFGSYRLGVHHKGADIDALCVAPRNIERTDYFQSFFEVLKKQPEVTECRSVEEAFVPVIKMNFDGIEIDLLFARLSLKEIPDDFDLRDDNLLRNLDHRSVRSLNGCRVTDEILALVPNIENFRLALRTIKLWAKKHGIYSNSLGYFGGVTWAMLVARTCQLYPNAAAATLVHKFFLVFSRWKWPNPVLLKHPDNVNLRFQVWDPRVNASDRYHLMPIITPAYPQQNSTFNVSESTKKVILTEFNRGMNITDEIMLGRIPWERLFEAPSFFYRYRHFIVLLVNSQTADDHLEWCGLVESKVRLLIGNLERNPHIALAHVNPKCFEFKKGQSANNSQNNSGNEDDLKQSQGNQSAVTSAPFCSMWFIGLEFERSENLNVDLTESIQNFTEHVMMHGVNIKMLKEGMTIDARHVKRKQLSLYLDSDFLKRERKSMESHNNFNNTLLANRKRLSTELAQSQDPLPPGQQPSSGNRGRDSGAKIQRLSDSLTEENSNASSDMGAGTPTTPTTAQLSAPSFKSSGKNGSEIDVVEQEPTQPHNNGNASSNTTTTEVACS

Target ORF information:

RefSeq Version	NM_166372.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hiiragi (hrg), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166372.3

ORF Insert Sequence:

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hrg ( NM_001274168.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261097.1 Drosophila melanogaster hiiragi (hrg), transcript variant D, mRNA.

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CloneID	OFa12857
Clone ID Related Accession (Same CDS sequence)	NM_001274168.2
Accession Version	NM_001274168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hiiragi, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274168.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA 
CAACCGCCAA CGCTACATCC GCATCCGCCA CATCAGCAGC CACCGCCAGC GCCGCCACAG 
CCACCAGCTC ACCATCATCA TCATCAGAAG TTCCGAAAGA ACAACAACGC GGCTGCGGCA 
GCCGCCTCCA ACAGCATTTT CAACCAACGC TCGATTCTGC ATGCCGCTTC CAGTCTATCG 
GCTGCTCTAA GCATTACCGG ACATAAGCGC AAGCAGCAAA CGGCAACATC AACAACCATG 
ATCAGCAGCA GCAACATTGC TCACAGCAAC TACACCCAGC ATAGCACTCA CAACAACAAC 
GGCGGTGGCG GCGGCAGCCA CTACAACAAA ACGTACTACA TTGATGATGA TGACGAGGAC 
AACAATCAAC CACTTTTGAC ACCTAACAAC CAAACACAAC AGCAACAAAA AGCGGTCAGA 
ATAACAGCTA CATCGGCGTC ACCAGCAACA TCGGTTCCGG TAGCTGCGAG TGCCGCACCA 
CCGGCACCGA CAATTTCTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261097.1
CDS	879..3362
Translation	MWNSEPTHRQHHQHNGNSTSGGPPAKQLGMTSAISLAEPRPEDLQRTDELRGSLEPYNVFESQDELNHRMEILAKLNTLVKQWVKEISVSKNMPESAAEKLGGKIYTFGSYRLGVHHKGADIDALCVAPRNIERTDYFQSFFEVLKKQPEVTECRSVEEAFVPVIKMNFDGIEIDLLFARLSLKEIPDDFDLRDDNLLRNLDHRSVRSLNGCRVTDEILALVPNIENFRLALRTIKLWAKKHGIYSNSLGYFGGVTWAMLVARTCQLYPNAAAATLVHKFFLVFSRWKWPNPVLLKHPDNVNLRFQVWDPRVNASDRYHLMPIITPAYPQQNSTFNVSESTKKVILTEFNRGMNITDEIMLGRIPWERLFEAPSFFYRYRHFIVLLVNSQTADDHLEWCGLVESKVRLLIGNLERNPHIALAHVNPKCFEFKKGQSANNSQNNSGNEDDLKQSQGNQSAVTSAPFCSMWFIGLEFERSENLNVDLTESIQNFTEHVMMHGVNIKMLKEGMTIDARHVKRKQLSLYLDSDFLKRERKSMESHNNFNNTLLANRKRLSTELAQSQDPLPPGQQPSSGNRGRDSGAKIQRLSDSLTEENSNASSDMGAGTPTTPTTAQLSAPSFKSSGKNGSEIDVVEQEPTQPHNNGNASSNTTTTEVACS*QPPTLHPHPPHQQPPPAPPQPPAHHHHHQKFRKNNNAAAAAASNSIFNQRSILHAASSLSAALSITGHKRKQQTATSTTMISSSNIAHSNYTQHSTHNNNGGGGGSHYNKTYYIDDDDEDNNQPLLTPNNQTQQQQKAVRITATSASPATSVPVAASAAPPAPTISL

Target ORF information:

RefSeq Version	NM_001274168.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hiiragi (hrg), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274168.2

ORF Insert Sequence:

ATGTGGAATT CCGAACCAAC ACATCGCCAG CACCATCAGC ACAATGGAAA TTCTACCTCG 
GGCGGACCGC CCGCCAAGCA ATTGGGCATG ACCTCGGCTA TCAGTCTGGC CGAGCCACGG 
CCTGAGGATC TCCAGCGCAC TGATGAGCTC CGCGGCTCAC TGGAGCCGTA CAACGTCTTT 
GAGAGCCAGG ACGAGCTAAA TCACCGTATG GAGATCCTGG CCAAGCTCAA CACACTGGTC 
AAGCAGTGGG TCAAGGAAAT CTCTGTCAGC AAGAACATGC CAGAGTCAGC CGCCGAGAAG 
CTTGGCGGCA AGATCTACAC ATTCGGCTCG TATCGATTGG GTGTCCATCA CAAGGGTGCC 
GATATAGATG CCTTATGTGT TGCTCCGCGA AATATTGAGC GCACCGACTA CTTTCAGAGC 
TTCTTTGAGG TGCTCAAGAA GCAGCCAGAG GTAACCGAGT GCCGCTCGGT GGAAGAGGCC 
TTTGTGCCCG TCATCAAAAT GAACTTTGAT GGCATCGAAA TTGATTTGCT GTTTGCCCGT 
CTGTCCCTAA AAGAAATTCC TGATGACTTT GATCTACGGG ATGATAACTT ACTGCGGAAT 
CTGGATCACC GTTCGGTGCG CAGCCTCAAC GGCTGTCGTG TGACGGACGA GATCCTGGCC 
CTGGTGCCCA ATATTGAAAA TTTCCGCCTG GCACTGCGCA CTATCAAATT GTGGGCAAAG 
AAGCACGGAA TCTACTCAAA TTCATTGGGT TATTTCGGTG GTGTGACCTG GGCCATGTTA 
GTGGCCAGAA CCTGTCAGCT GTATCCCAAC GCTGCGGCTG CCACACTGGT GCACAAGTTT 
TTCTTGGTCT TTTCGCGCTG GAAGTGGCCT AACCCAGTGC TACTCAAGCA CCCGGACAAC 
GTTAATCTAA GATTTCAAGT CTGGGATCCG CGTGTCAATG CCTCGGATCG GTATCATCTG 
ATGCCCATTA TTACGCCCGC ATACCCACAA CAGAATTCCA CATTTAATGT GTCCGAATCC 
ACCAAGAAGG TCATATTGAC CGAGTTCAAT CGCGGCATGA ACATCACGGA TGAAATCATG 
CTCGGTCGCA TTCCGTGGGA GCGTTTGTTT GAGGCACCTA GCTTTTTTTA CAGATACCGC 
CACTTTATCG TGTTGTTAGT CAACTCGCAG ACGGCTGACG ATCATTTAGA GTGGTGCGGC 
CTTGTTGAGT CAAAGGTGCG TCTGCTAATT GGCAACTTGG AGCGAAATCC CCATATTGCA 
CTGGCCCATG TCAATCCCAA GTGTTTTGAG TTTAAGAAGG GCCAAAGTGC CAACAACTCA 
CAAAACAACA GTGGAAATGA AGACGATCTG AAGCAGTCGC AGGGAAACCA GTCTGCTGTG 
ACATCGGCAC CCTTCTGCTC CATGTGGTTT ATAGGCTTGG AATTCGAGCG ATCGGAGAAC 
CTTAACGTGG ACCTCACCGA GAGCATACAA AACTTTACAG AGCATGTGAT GATGCACGGC 
GTTAACATCA AAATGCTTAA GGAAGGCATG ACCATTGATG CCCGCCATGT AAAGCGCAAG 
CAGCTGTCGC TTTATTTAGA CTCTGACTTC CTAAAACGTG AGCGCAAGTC AATGGAAAGT 
CACAATAATT TTAATAACAC GCTATTGGCT AACCGCAAAC GACTTTCTAC GGAACTGGCC 
CAATCGCAGG ATCCACTGCC CCCAGGCCAG CAGCCATCAA GTGGAAATCG CGGTCGGGAT 
AGCGGAGCCA AGATACAAAG ACTCAGCGAT TCGCTGACAG AGGAGAACTC GAATGCCTCC 
AGCGATATGG GGGCAGGGAC GCCCACCACG CCCACAACGG CGCAATTAAG TGCGCCAAGC 
TTTAAGAGCT CTGGCAAGAA CGGCTCCGAG ATAGACGTTG TAGAGCAGGA ACCGACACAG 
CCTCACAACA ATGGCAATGC CAGCAGCAAC ACAACCACAA CGGAGGTGGC ATGTTCGTGA 
CAACCGCCAA CGCTACATCC GCATCCGCCA CATCAGCAGC CACCGCCAGC GCCGCCACAG 
CCACCAGCTC ACCATCATCA TCATCAGAAG TTCCGAAAGA ACAACAACGC GGCTGCGGCA 
GCCGCCTCCA ACAGCATTTT CAACCAACGC TCGATTCTGC ATGCCGCTTC CAGTCTATCG 
GCTGCTCTAA GCATTACCGG ACATAAGCGC AAGCAGCAAA CGGCAACATC AACAACCATG 
ATCAGCAGCA GCAACATTGC TCACAGCAAC TACACCCAGC ATAGCACTCA CAACAACAAC 
GGCGGTGGCG GCGGCAGCCA CTACAACAAA ACGTACTACA TTGATGATGA TGACGAGGAC 
AACAATCAAC CACTTTTGAC ACCTAACAAC CAAACACAAC AGCAACAAAA AGCGGTCAGA 
ATAACAGCTA CATCGGCGTC ACCAGCAACA TCGGTTCCGG TAGCTGCGAG TGCCGCACCA 
CCGGCACCGA CAATTTCTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hrg cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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