Gprk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gprk2
Entrez Gene ID	49045
Full Name	G protein-coupled receptor kinase 2
Synonyms	154978_at,CG17998,Dmel\CG17998,Gprk-2,gprk2,nosh
General protein information	Preferred Names G protein-coupled receptor kinase 2 Names CG17998 gene product from transcript CG17998-RA CG17998-PA CG17998-PB Gprk2-PA Gprk2-PB nosh
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100C3-100C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057519.4	NP_476867.1	G protein-coupled receptor kinase 2, isoform A
NM_001276214.1	NP_001263143.1	G protein-coupled receptor kinase 2, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRK5	XP_003312835.1
Bos taurus (cattle)	GRK5	NP_776756.1
Xenopus tropicalis (tropical clawed frog)	LOC100497344	XP_002940394.1
	LOC100427251	XP_002805891.1
Mus musculus (house mouse)	Grk5	NP_061357.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004117	XP_312995.5
Caenorhabditis elegans (roundworm)	grk-1	NP_509676.1
Homo sapiens (human)	GRK5	NP_005299.1
Canis lupus familiaris (dog)	GRK5	XP_544045.3
Rattus norvegicus (Norway rat)	Grk5	NP_110456.1
Gallus gallus (chicken)	GRK5	XP_421789.2
Drosophila melanogaster (fruit fly)	Gprk2	NP_476867.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pleckstrin homology domain of Gprk2 essential for enriching PI(4)P and facilitating Smo activation
Title: PI(4)P Promotes Phosphorylation and Conformational Change of Smoothened through Interaction with Its C-terminal Tail.

Gprk2 phosphorylation in the membrane proximal C-terminus is an evolutionarily ancient mechanism of Smo regulation, and point to a higher degree of similarity in the regulation and signaling mechanisms of bilaterian Smo proteins
Title: A broadly conserved g-protein-coupled receptor kinase phosphorylation mechanism controls Drosophila smoothened activity.

based on findings, propose that Gprk2 attenuates and tunes Fog-Cta signaling to prevent apical constriction in lateral mesodermal cells and to support appropriate apical constriction in ventral mesodermal cells
Title: Gprk2 adjusts Fog signaling to organize cell movements in Drosophila gastrulation.

Drosophila G-protein-coupled receptor kinase 2 regulates cAMP-dependent Hedgehog signaling.
Title: Drosophila G-protein-coupled receptor kinase 2 regulates cAMP-dependent Hedgehog signaling.

G protein-coupled receptor kinase 2 promotes high-level Hedgehog signaling by regulating the active state of Smo through kinase-dependent and kinase-independent mechanisms in Drosophila
Title: G protein-coupled receptor kinase 2 promotes high-level Hedgehog signaling by regulating the active state of Smo through kinase-dependent and kinase-independent mechanisms in Drosophila.

The following Gprk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gprk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06941	NM_001276214.1 Latest version!	Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06941	NM_057519.4 Latest version!	Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gprk2 ( NM_057519.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476867.1 Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06941
Clone ID Related Accession (Same CDS sequence)	NM_057519.4 , NM_001276214.1
Accession Version	NM_057519.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein-coupled receptor kinase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057519.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTAG AGAATATTGT GGCCAATACG GTCTACTTGA AAGCGAGAGA AGGTGGTTCC 
GACAGCAACA AGGGAAAGAG CAAAAAATGG CGCAAAATAT TGCAATTCCC ACACATATCG 
CAATGCATCA ACCTGAAAGA CAAATTAGAT ATAAGCTATG GCTACGTGAT AGATCAGCAA 
CCCATTGGTC GTGAGCTCTT CCGTCTGTTT TGCGAGAACA AACGGCCCGT CTACTTTCGC 
TACATCACCT TCCTGGATGA GGTGGTCAAA TACGAGATCG AGTACATCTC GAACCGCATA 
TTCATTGGCC ATGACATCGG CCGTCGCTTC TTGGATGTCG AGGCACAGCT GGAGCTGCGC 
AACGGCAGCG GGGGCGACGC CTTGGACGCC GAGACGCAGG AGGAGCTGCT GCTCAACAGC 
AGCAATGCCA ATCCAACTGA AACAGCTGAG ACTGAACACT GCAACAATAC CACCGCCAAC 
AACTGCAACA ACATCAACAA CAGCAACAAC TCGCAGCACA GCAGCGACAT CAATCACAAG 
AAGCTGGACA CGCGCAATCA CAACGGCGAC GACGCCACTG GGAACGGGAG CAGCCACCAG 
GACGACGGGG ACGAGAGCGT CAAGTGCCAG GAGGGCCATG ACGATGCGGA AAAGGGTGGT 
GGCGGAGAGG GAGGCGGCGG CGGAAAGTGT GTCCCCGTGG GCGGGTATCC TGACGAACTC 
GTGTTGGACG TGCTTAACGA TGATCTCATT GCGCAAGTGC GTAACAAACT CAACAGCGGC 
GGCAAGGACA TCTTTGCCCA GTGTGTGAAC GCGGTTAAGG CGTTCCTGGC CGGCGAACCG 
TTCCGGGAGT TTGAGAGCTC TATGTATTTT CACCGATACT TGCAATGGAA GTGGCTTGAG 
GCGCAGCCCA TCACCTACAA AACGTTTCGC ATGTACCGGG TGCTCGGAAA GGGCGGCTTC 
GGCGAGGTGT GCGCCTGTCA GGTGCGGGCC ACTGGGAAAA TGTACGCGTG CAAAAAGCTG 
GAGAAAAAGC GCATCAAGAA GCGCAAGGGC GAGTCGATGG TGTTAATCGA GAAGCAGATA 
CTGCAGAAAA TCAACTCGCC GTTCGTGGTC AATCTGGCCT ACGCGTACGA GACAAAGGAC 
GCCCTCTGCC TGGTGCTGAC CATAATGAAT GGCGGCGATT TGAAATTCCA CATTTACAAC 
ATGGGCGGCG AACCAGGCTT TGAACTGGAG CGTGCCCGCT TCTATGCCGC CGAGGTGGCC 
TGTGGACTGC AGCACCTGCA CAAGCAGGGC ATCGTCTACC GGGATTGCAA GCCGGAAAAC 
ATCCTCCTTG ACGATCACGG CCATGTGCGC ATTTCCGACC TGGGACTGGC CGTCGAGATA 
CCGGAGGGCG AGATGGTGCG CGGCCGGGTG GGCACAGTGG GCTACATGGC CCCCGAGGTC 
ATCGACAACG AGAAGTACGC CTTCTCCCCG GACTGGTTCA GCTTCGGCTG CCTGCTGTAC 
GAGATGATCG AGGGCCAAGC GCCGTTCCGG ATGCGCAAGG AGAAGGTCAA GCGCGAGGAG 
GTGGACAGGC GCGTTAAGGA GGACCCCGAA AAGTATTCAA GCAAGTTTAA TGATGAAGCC 
AAATCAATGT GCCAACAGCT GTTAGCTAAA TCGATAAAGC AGCGCCTGGG CTGCCGCAAC 
GGTCGCATGG GCGGGCAGGA TGTGATGGCC CACCCGTTTT TCCACTCCAC CCAGCTCAAT 
TGGCGTCGCC TGGAGGCTGG TATGCTGGAG CCACCCTTTG TGCCAGACCC GCACGCCGTT 
TACGCCAAAG ATGTGCTCGA TATTGAACAG TTCTCTACGG TGAAGGGCGT CAATATCGAC 
GAATCCGACA CGAATTTCTA TACGAAATTC AACACAGGCT CCGTGTCCAT TTCCTGGCAG 
AACGAGATGA TGGAGACCGA GTGCTTTCGG GAACTGAATG TTTTTGGTCC CGAGGAGTGT 
CCCACGCCAG ATTTGCAAAT TAATGCAGCG CCCGAACCAG ACAAGGCGGG CTGTTTCCCC 
TTTCGCCGGA AGAAGAAGCA GCCGGCTCGC ACTCAGCCCA TACCCATTCC GGAGCATCTG 
TTAACCACTA GTCACAGCGT GTCCTCCACG ACGGTCGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476867.1
CDS	1139..3283
Translation	MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCINLKDKLDISYGYVIDQQPIGRELFRLFCENKRPVYFRYITFLDEVVKYEIEYISNRIFIGHDIGRRFLDVEAQLELRNGSGGDALDAETQEELLLNSSNANPTETAETEHCNNTTANNCNNINNSNNSQHSSDINHKKLDTRNHNGDDATGNGSSHQDDGDESVKCQEGHDDAEKGGGGEGGGGGKCVPVGGYPDELVLDVLNDDLIAQVRNKLNSGGKDIFAQCVNAVKAFLAGEPFREFESSMYFHRYLQWKWLEAQPITYKTFRMYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGESMVLIEKQILQKINSPFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGGEPGFELERARFYAAEVACGLQHLHKQGIVYRDCKPENILLDDHGHVRISDLGLAVEIPEGEMVRGRVGTVGYMAPEVIDNEKYAFSPDWFSFGCLLYEMIEGQAPFRMRKEKVKREEVDRRVKEDPEKYSSKFNDEAKSMCQQLLAKSIKQRLGCRNGRMGGQDVMAHPFFHSTQLNWRRLEAGMLEPPFVPDPHAVYAKDVLDIEQFSTVKGVNIDESDTNFYTKFNTGSVSISWQNEMMETECFRELNVFGPEECPTPDLQINAAPEPDKAGCFPFRRKKKQPARTQPIPIPEHLLTTSHSVSSTTVES

Target ORF information:

RefSeq Version	NM_057519.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057519.4

ORF Insert Sequence:

ATGGAATTAG AGAATATTGT GGCCAATACG GTCTACTTGA AAGCGAGAGA AGGTGGTTCC 
GACAGCAACA AGGGAAAGAG CAAAAAATGG CGCAAAATAT TGCAATTCCC ACACATATCG 
CAATGCATCA ACCTGAAAGA CAAATTAGAT ATAAGCTATG GCTACGTGAT AGATCAGCAA 
CCCATTGGTC GTGAGCTCTT CCGTCTGTTT TGCGAGAACA AACGGCCCGT CTACTTTCGC 
TACATCACCT TCCTGGATGA GGTGGTCAAA TACGAGATCG AGTACATCTC GAACCGCATA 
TTCATTGGCC ATGACATCGG CCGTCGCTTC TTGGATGTCG AGGCACAGCT GGAGCTGCGC 
AACGGCAGCG GGGGCGACGC CTTGGACGCC GAGACGCAGG AGGAGCTGCT GCTCAACAGC 
AGCAATGCCA ATCCAACTGA AACAGCTGAG ACTGAACACT GCAACAATAC CACCGCCAAC 
AACTGCAACA ACATCAACAA CAGCAACAAC TCGCAGCACA GCAGCGACAT CAATCACAAG 
AAGCTGGACA CGCGCAATCA CAACGGCGAC GACGCCACTG GGAACGGGAG CAGCCACCAG 
GACGACGGGG ACGAGAGCGT CAAGTGCCAG GAGGGCCATG ACGATGCGGA AAAGGGTGGT 
GGCGGAGAGG GAGGCGGCGG CGGAAAGTGT GTCCCCGTGG GCGGGTATCC TGACGAACTC 
GTGTTGGACG TGCTTAACGA TGATCTCATT GCGCAAGTGC GTAACAAACT CAACAGCGGC 
GGCAAGGACA TCTTTGCCCA GTGTGTGAAC GCGGTTAAGG CGTTCCTGGC CGGCGAACCG 
TTCCGGGAGT TTGAGAGCTC TATGTATTTT CACCGATACT TGCAATGGAA GTGGCTTGAG 
GCGCAGCCCA TCACCTACAA AACGTTTCGC ATGTACCGGG TGCTCGGAAA GGGCGGCTTC 
GGCGAGGTGT GCGCCTGTCA GGTGCGGGCC ACTGGGAAAA TGTACGCGTG CAAAAAGCTG 
GAGAAAAAGC GCATCAAGAA GCGCAAGGGC GAGTCGATGG TGTTAATCGA GAAGCAGATA 
CTGCAGAAAA TCAACTCGCC GTTCGTGGTC AATCTGGCCT ACGCGTACGA GACAAAGGAC 
GCCCTCTGCC TGGTGCTGAC CATAATGAAT GGCGGCGATT TGAAATTCCA CATTTACAAC 
ATGGGCGGCG AACCAGGCTT TGAACTGGAG CGTGCCCGCT TCTATGCCGC CGAGGTGGCC 
TGTGGACTGC AGCACCTGCA CAAGCAGGGC ATCGTCTACC GGGATTGCAA GCCGGAAAAC 
ATCCTCCTTG ACGATCACGG CCATGTGCGC ATTTCCGACC TGGGACTGGC CGTCGAGATA 
CCGGAGGGCG AGATGGTGCG CGGCCGGGTG GGCACAGTGG GCTACATGGC CCCCGAGGTC 
ATCGACAACG AGAAGTACGC CTTCTCCCCG GACTGGTTCA GCTTCGGCTG CCTGCTGTAC 
GAGATGATCG AGGGCCAAGC GCCGTTCCGG ATGCGCAAGG AGAAGGTCAA GCGCGAGGAG 
GTGGACAGGC GCGTTAAGGA GGACCCCGAA AAGTATTCAA GCAAGTTTAA TGATGAAGCC 
AAATCAATGT GCCAACAGCT GTTAGCTAAA TCGATAAAGC AGCGCCTGGG CTGCCGCAAC 
GGTCGCATGG GCGGGCAGGA TGTGATGGCC CACCCGTTTT TCCACTCCAC CCAGCTCAAT 
TGGCGTCGCC TGGAGGCTGG TATGCTGGAG CCACCCTTTG TGCCAGACCC GCACGCCGTT 
TACGCCAAAG ATGTGCTCGA TATTGAACAG TTCTCTACGG TGAAGGGCGT CAATATCGAC 
GAATCCGACA CGAATTTCTA TACGAAATTC AACACAGGCT CCGTGTCCAT TTCCTGGCAG 
AACGAGATGA TGGAGACCGA GTGCTTTCGG GAACTGAATG TTTTTGGTCC CGAGGAGTGT 
CCCACGCCAG ATTTGCAAAT TAATGCAGCG CCCGAACCAG ACAAGGCGGG CTGTTTCCCC 
TTTCGCCGGA AGAAGAAGCA GCCGGCTCGC ACTCAGCCCA TACCCATTCC GGAGCATCTG 
TTAACCACTA GTCACAGCGT GTCCTCCACG ACGGTCGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gprk2 ( NM_001276214.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263143.1 Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06941
Clone ID Related Accession (Same CDS sequence)	NM_057519.4 , NM_001276214.1
Accession Version	NM_001276214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein-coupled receptor kinase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTAG AGAATATTGT GGCCAATACG GTCTACTTGA AAGCGAGAGA AGGTGGTTCC 
GACAGCAACA AGGGAAAGAG CAAAAAATGG CGCAAAATAT TGCAATTCCC ACACATATCG 
CAATGCATCA ACCTGAAAGA CAAATTAGAT ATAAGCTATG GCTACGTGAT AGATCAGCAA 
CCCATTGGTC GTGAGCTCTT CCGTCTGTTT TGCGAGAACA AACGGCCCGT CTACTTTCGC 
TACATCACCT TCCTGGATGA GGTGGTCAAA TACGAGATCG AGTACATCTC GAACCGCATA 
TTCATTGGCC ATGACATCGG CCGTCGCTTC TTGGATGTCG AGGCACAGCT GGAGCTGCGC 
AACGGCAGCG GGGGCGACGC CTTGGACGCC GAGACGCAGG AGGAGCTGCT GCTCAACAGC 
AGCAATGCCA ATCCAACTGA AACAGCTGAG ACTGAACACT GCAACAATAC CACCGCCAAC 
AACTGCAACA ACATCAACAA CAGCAACAAC TCGCAGCACA GCAGCGACAT CAATCACAAG 
AAGCTGGACA CGCGCAATCA CAACGGCGAC GACGCCACTG GGAACGGGAG CAGCCACCAG 
GACGACGGGG ACGAGAGCGT CAAGTGCCAG GAGGGCCATG ACGATGCGGA AAAGGGTGGT 
GGCGGAGAGG GAGGCGGCGG CGGAAAGTGT GTCCCCGTGG GCGGGTATCC TGACGAACTC 
GTGTTGGACG TGCTTAACGA TGATCTCATT GCGCAAGTGC GTAACAAACT CAACAGCGGC 
GGCAAGGACA TCTTTGCCCA GTGTGTGAAC GCGGTTAAGG CGTTCCTGGC CGGCGAACCG 
TTCCGGGAGT TTGAGAGCTC TATGTATTTT CACCGATACT TGCAATGGAA GTGGCTTGAG 
GCGCAGCCCA TCACCTACAA AACGTTTCGC ATGTACCGGG TGCTCGGAAA GGGCGGCTTC 
GGCGAGGTGT GCGCCTGTCA GGTGCGGGCC ACTGGGAAAA TGTACGCGTG CAAAAAGCTG 
GAGAAAAAGC GCATCAAGAA GCGCAAGGGC GAGTCGATGG TGTTAATCGA GAAGCAGATA 
CTGCAGAAAA TCAACTCGCC GTTCGTGGTC AATCTGGCCT ACGCGTACGA GACAAAGGAC 
GCCCTCTGCC TGGTGCTGAC CATAATGAAT GGCGGCGATT TGAAATTCCA CATTTACAAC 
ATGGGCGGCG AACCAGGCTT TGAACTGGAG CGTGCCCGCT TCTATGCCGC CGAGGTGGCC 
TGTGGACTGC AGCACCTGCA CAAGCAGGGC ATCGTCTACC GGGATTGCAA GCCGGAAAAC 
ATCCTCCTTG ACGATCACGG CCATGTGCGC ATTTCCGACC TGGGACTGGC CGTCGAGATA 
CCGGAGGGCG AGATGGTGCG CGGCCGGGTG GGCACAGTGG GCTACATGGC CCCCGAGGTC 
ATCGACAACG AGAAGTACGC CTTCTCCCCG GACTGGTTCA GCTTCGGCTG CCTGCTGTAC 
GAGATGATCG AGGGCCAAGC GCCGTTCCGG ATGCGCAAGG AGAAGGTCAA GCGCGAGGAG 
GTGGACAGGC GCGTTAAGGA GGACCCCGAA AAGTATTCAA GCAAGTTTAA TGATGAAGCC 
AAATCAATGT GCCAACAGCT GTTAGCTAAA TCGATAAAGC AGCGCCTGGG CTGCCGCAAC 
GGTCGCATGG GCGGGCAGGA TGTGATGGCC CACCCGTTTT TCCACTCCAC CCAGCTCAAT 
TGGCGTCGCC TGGAGGCTGG TATGCTGGAG CCACCCTTTG TGCCAGACCC GCACGCCGTT 
TACGCCAAAG ATGTGCTCGA TATTGAACAG TTCTCTACGG TGAAGGGCGT CAATATCGAC 
GAATCCGACA CGAATTTCTA TACGAAATTC AACACAGGCT CCGTGTCCAT TTCCTGGCAG 
AACGAGATGA TGGAGACCGA GTGCTTTCGG GAACTGAATG TTTTTGGTCC CGAGGAGTGT 
CCCACGCCAG ATTTGCAAAT TAATGCAGCG CCCGAACCAG ACAAGGCGGG CTGTTTCCCC 
TTTCGCCGGA AGAAGAAGCA GCCGGCTCGC ACTCAGCCCA TACCCATTCC GGAGCATCTG 
TTAACCACTA GTCACAGCGT GTCCTCCACG ACGGTCGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263143.1
CDS	1091..3235
Translation	MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCINLKDKLDISYGYVIDQQPIGRELFRLFCENKRPVYFRYITFLDEVVKYEIEYISNRIFIGHDIGRRFLDVEAQLELRNGSGGDALDAETQEELLLNSSNANPTETAETEHCNNTTANNCNNINNSNNSQHSSDINHKKLDTRNHNGDDATGNGSSHQDDGDESVKCQEGHDDAEKGGGGEGGGGGKCVPVGGYPDELVLDVLNDDLIAQVRNKLNSGGKDIFAQCVNAVKAFLAGEPFREFESSMYFHRYLQWKWLEAQPITYKTFRMYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGESMVLIEKQILQKINSPFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGGEPGFELERARFYAAEVACGLQHLHKQGIVYRDCKPENILLDDHGHVRISDLGLAVEIPEGEMVRGRVGTVGYMAPEVIDNEKYAFSPDWFSFGCLLYEMIEGQAPFRMRKEKVKREEVDRRVKEDPEKYSSKFNDEAKSMCQQLLAKSIKQRLGCRNGRMGGQDVMAHPFFHSTQLNWRRLEAGMLEPPFVPDPHAVYAKDVLDIEQFSTVKGVNIDESDTNFYTKFNTGSVSISWQNEMMETECFRELNVFGPEECPTPDLQINAAPEPDKAGCFPFRRKKKQPARTQPIPIPEHLLTTSHSVSSTTVES

Target ORF information:

RefSeq Version	NM_001276214.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein-coupled receptor kinase 2 (Gprk2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276214.1

ORF Insert Sequence:

ATGGAATTAG AGAATATTGT GGCCAATACG GTCTACTTGA AAGCGAGAGA AGGTGGTTCC 
GACAGCAACA AGGGAAAGAG CAAAAAATGG CGCAAAATAT TGCAATTCCC ACACATATCG 
CAATGCATCA ACCTGAAAGA CAAATTAGAT ATAAGCTATG GCTACGTGAT AGATCAGCAA 
CCCATTGGTC GTGAGCTCTT CCGTCTGTTT TGCGAGAACA AACGGCCCGT CTACTTTCGC 
TACATCACCT TCCTGGATGA GGTGGTCAAA TACGAGATCG AGTACATCTC GAACCGCATA 
TTCATTGGCC ATGACATCGG CCGTCGCTTC TTGGATGTCG AGGCACAGCT GGAGCTGCGC 
AACGGCAGCG GGGGCGACGC CTTGGACGCC GAGACGCAGG AGGAGCTGCT GCTCAACAGC 
AGCAATGCCA ATCCAACTGA AACAGCTGAG ACTGAACACT GCAACAATAC CACCGCCAAC 
AACTGCAACA ACATCAACAA CAGCAACAAC TCGCAGCACA GCAGCGACAT CAATCACAAG 
AAGCTGGACA CGCGCAATCA CAACGGCGAC GACGCCACTG GGAACGGGAG CAGCCACCAG 
GACGACGGGG ACGAGAGCGT CAAGTGCCAG GAGGGCCATG ACGATGCGGA AAAGGGTGGT 
GGCGGAGAGG GAGGCGGCGG CGGAAAGTGT GTCCCCGTGG GCGGGTATCC TGACGAACTC 
GTGTTGGACG TGCTTAACGA TGATCTCATT GCGCAAGTGC GTAACAAACT CAACAGCGGC 
GGCAAGGACA TCTTTGCCCA GTGTGTGAAC GCGGTTAAGG CGTTCCTGGC CGGCGAACCG 
TTCCGGGAGT TTGAGAGCTC TATGTATTTT CACCGATACT TGCAATGGAA GTGGCTTGAG 
GCGCAGCCCA TCACCTACAA AACGTTTCGC ATGTACCGGG TGCTCGGAAA GGGCGGCTTC 
GGCGAGGTGT GCGCCTGTCA GGTGCGGGCC ACTGGGAAAA TGTACGCGTG CAAAAAGCTG 
GAGAAAAAGC GCATCAAGAA GCGCAAGGGC GAGTCGATGG TGTTAATCGA GAAGCAGATA 
CTGCAGAAAA TCAACTCGCC GTTCGTGGTC AATCTGGCCT ACGCGTACGA GACAAAGGAC 
GCCCTCTGCC TGGTGCTGAC CATAATGAAT GGCGGCGATT TGAAATTCCA CATTTACAAC 
ATGGGCGGCG AACCAGGCTT TGAACTGGAG CGTGCCCGCT TCTATGCCGC CGAGGTGGCC 
TGTGGACTGC AGCACCTGCA CAAGCAGGGC ATCGTCTACC GGGATTGCAA GCCGGAAAAC 
ATCCTCCTTG ACGATCACGG CCATGTGCGC ATTTCCGACC TGGGACTGGC CGTCGAGATA 
CCGGAGGGCG AGATGGTGCG CGGCCGGGTG GGCACAGTGG GCTACATGGC CCCCGAGGTC 
ATCGACAACG AGAAGTACGC CTTCTCCCCG GACTGGTTCA GCTTCGGCTG CCTGCTGTAC 
GAGATGATCG AGGGCCAAGC GCCGTTCCGG ATGCGCAAGG AGAAGGTCAA GCGCGAGGAG 
GTGGACAGGC GCGTTAAGGA GGACCCCGAA AAGTATTCAA GCAAGTTTAA TGATGAAGCC 
AAATCAATGT GCCAACAGCT GTTAGCTAAA TCGATAAAGC AGCGCCTGGG CTGCCGCAAC 
GGTCGCATGG GCGGGCAGGA TGTGATGGCC CACCCGTTTT TCCACTCCAC CCAGCTCAAT 
TGGCGTCGCC TGGAGGCTGG TATGCTGGAG CCACCCTTTG TGCCAGACCC GCACGCCGTT 
TACGCCAAAG ATGTGCTCGA TATTGAACAG TTCTCTACGG TGAAGGGCGT CAATATCGAC 
GAATCCGACA CGAATTTCTA TACGAAATTC AACACAGGCT CCGTGTCCAT TTCCTGGCAG 
AACGAGATGA TGGAGACCGA GTGCTTTCGG GAACTGAATG TTTTTGGTCC CGAGGAGTGT 
CCCACGCCAG ATTTGCAAAT TAATGCAGCG CCCGAACCAG ACAAGGCGGG CTGTTTCCCC 
TTTCGCCGGA AGAAGAAGCA GCCGGCTCGC ACTCAGCCCA TACCCATTCC GGAGCATCTG 
TTAACCACTA GTCACAGCGT GTCCTCCACG ACGGTCGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gprk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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