Pgant35A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pgant35A
Entrez Gene ID	48775
Full Name	Polypeptide N-acetylgalactosaminyltransferase 35A
Synonyms	A3,BG:DS01068.8,CG7480,Dmel\CG7480,GalNAc-T,br12,dGalNAc-T1,l(2)35Aa,l(2)br12,l35Aa,pgant35,pgant35A
General protein information	Preferred Names Polypeptide N-acetylgalactosaminyltransferase 35A Names CG7480 gene product from transcript CG7480-RA CG7480-PA Pgant35A-PA lethal 12 in the black-reduced region lethal group A3 polypeptide N-acetylgalactosaminyltransferase 35A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35A4-35A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_143812.4	NP_652069.2	polypeptide N-acetylgalactosaminyltransferase 35A

Pathways from BioSystems

Homologies

	GALNT11	XP_001105286.1
Danio rerio (zebrafish)	galnt11	NP_001070030.2
Caenorhabditis elegans (roundworm)	gly-11	NP_001022948.1
Pan troglodytes (chimpanzee)	GALNT11	XP_001143140.1
Mus musculus (house mouse)	Galnt11	NP_659157.1
Gallus gallus (chicken)	GALNT11	XP_418541.2
Drosophila melanogaster (fruit fly)	Pgant35A	NP_652069.2
Homo sapiens (human)	GALNT11	NP_071370.2
Bos taurus (cattle)	GALNT11	XP_005206020.1
Rattus norvegicus (Norway rat)	Galnt11	NP_001258248.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004429	XP_313714.5
Canis lupus familiaris (dog)	GALNT11	XP_005629753.1
Xenopus tropicalis (tropical clawed frog)	galnt11	NP_001006904.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila embryogenesis requires functional pgant35A; the presence of this gene product during fly embryogenesis is functionally distinct from other Drosophila GalNAc-transferase isoforms and from the proposed human ortholog GALNT11.
Title: Rescue of Drosophila Melanogaster l(2)35Aa lethality is only mediated by polypeptide GalNAc-transferase pgant35A, but not by the evolutionary conserved human ortholog GalNAc-transferase-T11.

essential for viability in Drosophila melanogaster
Title: A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is essential for viability in Drosophila melanogaster.

The following Pgant35A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pgant35A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15270	NM_143812.4 Latest version!	Drosophila melanogaster polypeptide N-acetylgalactosaminyltransferase 35A (Pgant35A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pgant35A ( NM_143812.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652069.2 Drosophila melanogaster polypeptide N-acetylgalactosaminyltransferase 35A (Pgant35A), mRNA.

Price & Availability↑

CloneID	OFa15270
Clone ID Related Accession (Same CDS sequence)	NM_143812.4
Accession Version	NM_143812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polypeptide N-acetylgalactosaminyltransferase 35A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_143812.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCAAA TCAAGCGCCT CCTGTGCAAA TCCTGCGGCC TGGGCACCCT TCTGGTGGCG 
GTCGTCTGGC TGCTGGCGCT GCTTTTCTAC TCGCACAGCC TGCGCAGCTC CATCCGCTCG 
GCAGGATGGA GGATCGACGA AGGGAACGCA ACGCCACGGG CCGAGCTGAG CTACCAGGCC 
AGGGTCACCG TCGGATGCAC TCCAAATGCC AGCATTACCA CTGGCGAATC GCCGGCGGCA 
CCCAAGCCGC CCAGTGATCC GGAGCAATTG GAACTGTTGG GTGTGGTGCG CAACAAGCAG 
GACAAGTACA TACGCGACAT TGGCTACAAG CATCACGCCT TCAACGCGCT GGTGTCCAAT 
AACATAGGAC TCTTCCGGGC GATCCCAGAC ACTCGGCACA AGGTGTGCGA TCGCCAGGAG 
ACCACGGAAG CGGAAAATCT GCCCCAGGCC TCCATAGTCA TGTGCTTCTA CAACGAGCAC 
AAGATGACGC TGATGCGCTC GATTAAGACC GTGCTGGAAC GCACGCCCAG CTACCTGCTG 
CGCGAGATTA TTCTGGTGGA TGATCACAGC GATCTTCCAG AATTGGAATT CCATTTGCAC 
GGGGATCTGC GTGCCCGCCT GAAGTACGAC AACCTGCGGT ACATCAAAAA CGAGCAGCGC 
GAGGGTCTCA TTCGTTCCCG GGTCATTGGT GCACGCGAGG CCGTGGGCGA TGTGCTCGTC 
TTCCTCGACT CGCATATCGA GGTCAATCAG CAGTGGCTGG AGCCGCTGCT GCGCCTCATC 
AAGTCGGAGA ACGCCACTCT GGCCGTGCCC GTCATCGACC TGATCAACGC TGATACCTTT 
GAGTACACGC CCAGTCCACT GGTGCGCGGA GGATTCAACT GGGGCCTGCA CTTCCGCTGG 
GAGAATCTGC CCGAGGGCAC GCTCAAGGTG CCGGAGGATT TTCGCGGACC CTTCCGTTCG 
CCCACCATGG CCGGCGGATT GTTTGCCGTG AACAGGAAGT ACTTCCAGCA TCTGGGCGAG 
TACGACATGG CCATGGATAT ATGGGGTGGC GAGAATATAG AGATCTCCTT CAGAGCCTGG 
CAATGCGGTG GCGCCATTAA GATCGTTCCC TGCTCCCGTG TCGGTCACAT ATTCCGCAAA 
CGACGTCCGT ACACTTCGCC AGATGGAGCC AACACCATGT TGAAGAACTC ACTGCGTTTG 
GCGCACGTCT GGATGGATCA GTACAAGGAT TACTATCTCA AGCACGAAAA GGTGCCCAAG 
ACCTATGATT ATGGAGACAT TAGCGATCGC CTCAAGCTTA GGGAGCGTCT GCAGTGCAGG 
GACTTTGCCT GGTATCTAAA GAATGTCTAT CCAGAGCTGC ATGTGCCGGG CGAGGAGTCC 
AAGAAGTCGG CGGCAGCGCC CATCTTTCAG CCTTGGCACT CGCGAAAACG TAACTATGTC 
GACACATTCC AGCTGCGACT GACGGGAACG GAGCTCTGTG CCGCCGTGGT GGCTCCCAAG 
GTGAAGGGTT TCTGGAAGAA GGGCAGCTCC CTGCAATTGC AAACCTGCCG AAGGACACCC 
AATCAACTGT GGTACGAAAC GGAGAAGGCG GAAATTGTGC TGGACAAGTT GCTGTGCCTG 
GAGGCATCCG GGGATGCGCA GGTCACGGTC AACAAGTGCC ACGAGATGCT CGGCGATCAG 
CAGTGGCGGC ACACCCGCAA CGCCAACAGT CCCGTCTACA ACATGGCCAA AGGCACCTGC 
CTCCGGGCAG CTGCTCCCAC GACGGGCGCC CTCATCAGTC TGGATCTGTG CTCCAAGTCC 
AACGGAGCGG GCGGCTCCTG GGACATTGTG CAGCTGAAGA AGCCAACGGA GGCGGAGGGC 
AGGGCCAAGG AGGCGAGGAA CTCGGACAAG GCGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652069.2
CDS	129..2027
Translation	MMQIKRLLCKSCGLGTLLVAVVWLLALLFYSHSLRSSIRSAGWRIDEGNATPRAELSYQARVTVGCTPNASITTGESPAAPKPPSDPEQLELLGVVRNKQDKYIRDIGYKHHAFNALVSNNIGLFRAIPDTRHKVCDRQETTEAENLPQASIVMCFYNEHKMTLMRSIKTVLERTPSYLLREIILVDDHSDLPELEFHLHGDLRARLKYDNLRYIKNEQREGLIRSRVIGAREAVGDVLVFLDSHIEVNQQWLEPLLRLIKSENATLAVPVIDLINADTFEYTPSPLVRGGFNWGLHFRWENLPEGTLKVPEDFRGPFRSPTMAGGLFAVNRKYFQHLGEYDMAMDIWGGENIEISFRAWQCGGAIKIVPCSRVGHIFRKRRPYTSPDGANTMLKNSLRLAHVWMDQYKDYYLKHEKVPKTYDYGDISDRLKLRERLQCRDFAWYLKNVYPELHVPGEESKKSAAAPIFQPWHSRKRNYVDTFQLRLTGTELCAAVVAPKVKGFWKKGSSLQLQTCRRTPNQLWYETEKAEIVLDKLLCLEASGDAQVTVNKCHEMLGDQQWRHTRNANSPVYNMAKGTCLRAAAPTTGALISLDLCSKSNGAGGSWDIVQLKKPTEAEGRAKEARNSDKAL

Target ORF information:

RefSeq Version	NM_143812.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polypeptide N-acetylgalactosaminyltransferase 35A (Pgant35A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143812.4

ORF Insert Sequence:

ATGATGCAAA TCAAGCGCCT CCTGTGCAAA TCCTGCGGCC TGGGCACCCT TCTGGTGGCG 
GTCGTCTGGC TGCTGGCGCT GCTTTTCTAC TCGCACAGCC TGCGCAGCTC CATCCGCTCG 
GCAGGATGGA GGATCGACGA AGGGAACGCA ACGCCACGGG CCGAGCTGAG CTACCAGGCC 
AGGGTCACCG TCGGATGCAC TCCAAATGCC AGCATTACCA CTGGCGAATC GCCGGCGGCA 
CCCAAGCCGC CCAGTGATCC GGAGCAATTG GAACTGTTGG GTGTGGTGCG CAACAAGCAG 
GACAAGTACA TACGCGACAT TGGCTACAAG CATCACGCCT TCAACGCGCT GGTGTCCAAT 
AACATAGGAC TCTTCCGGGC GATCCCAGAC ACTCGGCACA AGGTGTGCGA TCGCCAGGAG 
ACCACGGAAG CGGAAAATCT GCCCCAGGCC TCCATAGTCA TGTGCTTCTA CAACGAGCAC 
AAGATGACGC TGATGCGCTC GATTAAGACC GTGCTGGAAC GCACGCCCAG CTACCTGCTG 
CGCGAGATTA TTCTGGTGGA TGATCACAGC GATCTTCCAG AATTGGAATT CCATTTGCAC 
GGGGATCTGC GTGCCCGCCT GAAGTACGAC AACCTGCGGT ACATCAAAAA CGAGCAGCGC 
GAGGGTCTCA TTCGTTCCCG GGTCATTGGT GCACGCGAGG CCGTGGGCGA TGTGCTCGTC 
TTCCTCGACT CGCATATCGA GGTCAATCAG CAGTGGCTGG AGCCGCTGCT GCGCCTCATC 
AAGTCGGAGA ACGCCACTCT GGCCGTGCCC GTCATCGACC TGATCAACGC TGATACCTTT 
GAGTACACGC CCAGTCCACT GGTGCGCGGA GGATTCAACT GGGGCCTGCA CTTCCGCTGG 
GAGAATCTGC CCGAGGGCAC GCTCAAGGTG CCGGAGGATT TTCGCGGACC CTTCCGTTCG 
CCCACCATGG CCGGCGGATT GTTTGCCGTG AACAGGAAGT ACTTCCAGCA TCTGGGCGAG 
TACGACATGG CCATGGATAT ATGGGGTGGC GAGAATATAG AGATCTCCTT CAGAGCCTGG 
CAATGCGGTG GCGCCATTAA GATCGTTCCC TGCTCCCGTG TCGGTCACAT ATTCCGCAAA 
CGACGTCCGT ACACTTCGCC AGATGGAGCC AACACCATGT TGAAGAACTC ACTGCGTTTG 
GCGCACGTCT GGATGGATCA GTACAAGGAT TACTATCTCA AGCACGAAAA GGTGCCCAAG 
ACCTATGATT ATGGAGACAT TAGCGATCGC CTCAAGCTTA GGGAGCGTCT GCAGTGCAGG 
GACTTTGCCT GGTATCTAAA GAATGTCTAT CCAGAGCTGC ATGTGCCGGG CGAGGAGTCC 
AAGAAGTCGG CGGCAGCGCC CATCTTTCAG CCTTGGCACT CGCGAAAACG TAACTATGTC 
GACACATTCC AGCTGCGACT GACGGGAACG GAGCTCTGTG CCGCCGTGGT GGCTCCCAAG 
GTGAAGGGTT TCTGGAAGAA GGGCAGCTCC CTGCAATTGC AAACCTGCCG AAGGACACCC 
AATCAACTGT GGTACGAAAC GGAGAAGGCG GAAATTGTGC TGGACAAGTT GCTGTGCCTG 
GAGGCATCCG GGGATGCGCA GGTCACGGTC AACAAGTGCC ACGAGATGCT CGGCGATCAG 
CAGTGGCGGC ACACCCGCAA CGCCAACAGT CCCGTCTACA ACATGGCCAA AGGCACCTGC 
CTCCGGGCAG CTGCTCCCAC GACGGGCGCC CTCATCAGTC TGGATCTGTG CTCCAAGTCC 
AACGGAGCGG GCGGCTCCTG GGACATTGTG CAGCTGAAGA AGCCAACGGA GGCGGAGGGC 
AGGGCCAAGG AGGCGAGGAA CTCGGACAAG GCGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pgant35A cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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