Hrb87F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hrb87F
Entrez Gene ID	48535
Full Name	Heterogeneous nuclear ribonucleoprotein at 87F
Synonyms	CG12749,Dmel\CG12749,HRB87F,HRB87F/hrp36,HRP36,Hrb2,Hrb85CD,Hrb87Fa,Hrb87f,Hrp36,P11,P11/Hrb87F,Q14,Q16,hnRNP A2/B1,hnRNP36,hnRNPA1,hnRNPA1/A2,hnRNPA2,hrp36,p38
General protein information	Preferred Names Heterogeneous nuclear ribonucleoprotein at 87F Names CG12749 gene product from transcript CG12749-RD CG12749-PA CG12749-PC CG12749-PD CG12749-PE Hrb87F-PA Hrb87F-PC Hrb87F-PD Hrb87F-PE heterogeneous nuclear ribonucleoprotein at 85CD heterogeneous nuclear ribonucleoprotein at 87F hnRNA-binding protein 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87F7-87F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275609.1	NP_001262538.1	heterogeneous nuclear ribonucleoprotein at 87F, isoform D
NM_057458.5	NP_476806.2	heterogeneous nuclear ribonucleoprotein at 87F, isoform E
NM_057459.5	NP_476807.1	heterogeneous nuclear ribonucleoprotein at 87F, isoform A
NM_001170131.2	NP_001163602.1	heterogeneous nuclear ribonucleoprotein at 87F, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The hnRNP A1 homolog Hrb87F/Hrp36 is important for telomere maintenance in Drosophila melanogaster.
Title: The hnRNP A1 homolog Hrb87F/Hrp36 is important for telomere maintenance in Drosophila melanogaster.

Hrp36 is essential for normal development and for survival under conditions of stress
Title: The hnRNP A1 homolog Hrp36 is essential for normal development, female fecundity, omega speckle formation and stress tolerance in Drosophila melanogaster.

direct and indirect interactions of the hsromega transcripts with a variety of regulatory proteins like hnRNPs, CBP, proteasome, Drosophila inhibitor of apoptosis protein 1 (DIAP1)
Title: Improved activities of CREB binding protein, heterogeneous nuclear ribonucleoproteins and proteasome following downregulation of noncoding hsromega transcripts help suppress poly(Q) pathogenesis in fly models.

shows that the heterogeneous nuclear ribonucleoprotein hrp36 acts specifically within, and throughout, the exon 6 cluster to prevent the inclusion of multiple exons.
Title: A regulator of Dscam mutually exclusive splicing fidelity.

The following Hrb87F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hrb87F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06930	NM_001275609.1 Latest version!	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant D (Hrb87F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa06930	NM_057459.5 Latest version!	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant A (Hrb87F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa06930	NM_057458.5 Latest version!	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant E (Hrb87F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa06930	NM_001170131.2 Latest version!	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant C (Hrb87F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hrb87F ( NM_001275609.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262538.1 Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant D (Hrb87F), mRNA.

Price & Availability↑

CloneID	OFa06930
Clone ID Related Accession (Same CDS sequence)	NM_001275609.1 , NM_057458.5 , NM_057459.5 , NM_001170131.2
Accession Version	NM_001275609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heterogeneous nuclear ribonucleoprotein at 87F, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262538.1
CDS	145..1302
Translation	MAEQNDSNGNYDDGEEITEPEQLRKLFIGGLDYRTTDDGLKAHFEKWGNIVDVVVMKDPKTKRSRGFGFITYSQSYMIDNAQNARPHKIDGRTVEPKRAVPRQEIDSPNAGATVKKLFVGGLRDDHDEECLREYFKDFGQIVSVNIVSDKDTGKKRGFAFIEFDDYDPVDKIILQKTHSIKNKTLDVKKAIAKQDMDRQGGGGGRGGPRAGGRGGQGDRGQGGGGWGGQNRQNGGGNWGGAGGGGGFGNSGGNFGGGQGGGSGGWNQQGGSGGGPWNNQGGGNGGWNGGGGGGYGGGNSNGSWGGNGGGGGGGGGFGNEYQQSYGGGPQRNSNFGNNRPAPYSQGGGGGGFNKGNQGGGQGFAGNNYNTGGGGQGGNMGGGNRRY

Target ORF information:

RefSeq Version	NM_001275609.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant D (Hrb87F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275609.1

ORF Insert Sequence:

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrb87F ( NM_057458.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476806.2 Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant E (Hrb87F), mRNA.

Price & Availability↑

CloneID	OFa06930
Clone ID Related Accession (Same CDS sequence)	NM_001275609.1 , NM_057458.5 , NM_057459.5 , NM_001170131.2
Accession Version	NM_057458.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heterogeneous nuclear ribonucleoprotein at 87F, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057458.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476806.2
CDS	145..1302
Translation	MAEQNDSNGNYDDGEEITEPEQLRKLFIGGLDYRTTDDGLKAHFEKWGNIVDVVVMKDPKTKRSRGFGFITYSQSYMIDNAQNARPHKIDGRTVEPKRAVPRQEIDSPNAGATVKKLFVGGLRDDHDEECLREYFKDFGQIVSVNIVSDKDTGKKRGFAFIEFDDYDPVDKIILQKTHSIKNKTLDVKKAIAKQDMDRQGGGGGRGGPRAGGRGGQGDRGQGGGGWGGQNRQNGGGNWGGAGGGGGFGNSGGNFGGGQGGGSGGWNQQGGSGGGPWNNQGGGNGGWNGGGGGGYGGGNSNGSWGGNGGGGGGGGGFGNEYQQSYGGGPQRNSNFGNNRPAPYSQGGGGGGFNKGNQGGGQGFAGNNYNTGGGGQGGNMGGGNRRY

Target ORF information:

RefSeq Version	NM_057458.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant E (Hrb87F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057458.5

ORF Insert Sequence:

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrb87F ( NM_057459.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476807.1 Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant A (Hrb87F), mRNA.

Price & Availability↑

CloneID	OFa06930
Clone ID Related Accession (Same CDS sequence)	NM_001275609.1 , NM_057458.5 , NM_057459.5 , NM_001170131.2
Accession Version	NM_057459.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heterogeneous nuclear ribonucleoprotein at 87F, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057459.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476807.1
CDS	145..1302
Translation	MAEQNDSNGNYDDGEEITEPEQLRKLFIGGLDYRTTDDGLKAHFEKWGNIVDVVVMKDPKTKRSRGFGFITYSQSYMIDNAQNARPHKIDGRTVEPKRAVPRQEIDSPNAGATVKKLFVGGLRDDHDEECLREYFKDFGQIVSVNIVSDKDTGKKRGFAFIEFDDYDPVDKIILQKTHSIKNKTLDVKKAIAKQDMDRQGGGGGRGGPRAGGRGGQGDRGQGGGGWGGQNRQNGGGNWGGAGGGGGFGNSGGNFGGGQGGGSGGWNQQGGSGGGPWNNQGGGNGGWNGGGGGGYGGGNSNGSWGGNGGGGGGGGGFGNEYQQSYGGGPQRNSNFGNNRPAPYSQGGGGGGFNKGNQGGGQGFAGNNYNTGGGGQGGNMGGGNRRY

Target ORF information:

RefSeq Version	NM_057459.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant A (Hrb87F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057459.5

ORF Insert Sequence:

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrb87F ( NM_001170131.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163602.1 Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant C (Hrb87F), mRNA.

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CloneID	OFa06930
Clone ID Related Accession (Same CDS sequence)	NM_001275609.1 , NM_057458.5 , NM_057459.5 , NM_001170131.2
Accession Version	NM_001170131.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heterogeneous nuclear ribonucleoprotein at 87F, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170131.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163602.1
CDS	145..1302
Translation	MAEQNDSNGNYDDGEEITEPEQLRKLFIGGLDYRTTDDGLKAHFEKWGNIVDVVVMKDPKTKRSRGFGFITYSQSYMIDNAQNARPHKIDGRTVEPKRAVPRQEIDSPNAGATVKKLFVGGLRDDHDEECLREYFKDFGQIVSVNIVSDKDTGKKRGFAFIEFDDYDPVDKIILQKTHSIKNKTLDVKKAIAKQDMDRQGGGGGRGGPRAGGRGGQGDRGQGGGGWGGQNRQNGGGNWGGAGGGGGFGNSGGNFGGGQGGGSGGWNQQGGSGGGPWNNQGGGNGGWNGGGGGGYGGGNSNGSWGGNGGGGGGGGGFGNEYQQSYGGGPQRNSNFGNNRPAPYSQGGGGGGFNKGNQGGGQGFAGNNYNTGGGGQGGNMGGGNRRY

Target ORF information:

RefSeq Version	NM_001170131.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heterogeneous nuclear ribonucleoprotein at 87F, transcript variant C (Hrb87F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170131.2

ORF Insert Sequence:

ATGGCGGAAC AAAACGATTC CAACGGAAAC TACGACGATG GTGAAGAGAT CACCGAGCCA 
GAGCAGCTGC GCAAACTGTT CATCGGCGGA CTGGACTACC GCACCACAGA TGATGGCCTG 
AAGGCTCACT TCGAGAAGTG GGGCAACATT GTCGACGTGG TGGTGATGAA GGATCCCAAG 
ACGAAGCGCT CTCGCGGCTT CGGTTTCATC ACGTACTCCC AGTCGTACAT GATCGACAAT 
GCGCAGAATG CCAGGCCACA CAAGATCGAT GGACGCACCG TGGAGCCCAA GAGGGCTGTG 
CCACGCCAGG AGATCGATTC CCCGAATGCG GGAGCCACGG TAAAGAAGCT CTTTGTGGGC 
GGGCTTCGAG ACGATCACGA TGAAGAGTGC CTGCGCGAGT ACTTCAAGGA CTTTGGCCAG 
ATCGTGAGCG TGAACATTGT TTCCGACAAG GACACCGGCA AGAAGCGCGG CTTCGCCTTC 
ATTGAGTTCG ATGACTACGA TCCCGTTGAC AAAATCATCC TTCAGAAGAC CCACTCCATC 
AAGAACAAGA CCCTGGACGT GAAGAAGGCT ATTGCCAAGC AGGATATGGA TCGACAGGGC 
GGAGGTGGCG GACGCGGAGG TCCTCGAGCT GGCGGTCGCG GTGGTCAGGG TGACCGCGGC 
CAGGGAGGCG GTGGCTGGGG AGGCCAGAAC AGACAGAACG GTGGGGGCAA CTGGGGCGGA 
GCTGGCGGCG GCGGAGGATT CGGCAACAGC GGCGGTAACT TTGGAGGCGG TCAGGGCGGC 
GGCTCTGGCG GTTGGAATCA GCAAGGCGGA AGCGGAGGTG GTCCATGGAA TAACCAGGGT 
GGCGGCAACG GCGGCTGGAA CGGTGGTGGT GGCGGCGGCT ACGGCGGCGG AAACAGCAAT 
GGCAGCTGGG GCGGTAACGG TGGTGGAGGT GGTGGTGGCG GTGGCTTCGG AAATGAATAC 
CAGCAGAGCT ACGGCGGCGG TCCACAGCGC AACAGCAACT TTGGCAACAA CCGTCCAGCT 
CCTTACAGTC AAGGAGGTGG TGGTGGAGGA TTCAACAAGG GTAACCAGGG TGGAGGTCAA 
GGCTTTGCTG GCAACAACTA CAACACCGGA GGTGGTGGCC AGGGTGGAAA TATGGGAGGC 
GGCAATAGAC GTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hrb87F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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