Pkc53E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkc53E
Entrez Gene ID	48311
Full Name	Protein C kinase 53E
Synonyms	CG6622,DPKC,Dmel\CG6622,Dpkc1,PK-C,PKC,PKC 53B,PKC 53E,PKC-53B,PKC-53E,PKC1,PKC53E,PKC53e,Pkc1,dPKC,dPKC53E(br),pkc53e
General protein information	Preferred Names Protein C kinase 53E Names CG6622 gene product from transcript CG6622-RF CG6622-PA CG6622-PB CG6622-PC CG6622-PD CG6622-PE CG6622-PF Pkc53E-PA Pkc53E-PB Pkc53E-PC Pkc53E-PD Pkc53E-PE Pkc53E-PF protein C kinase 53E protein kinase C protein kinase-C 53E protein kinase-c1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53E2-53E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259448.1	NP_001246377.1	protein C kinase 53E, isoform D
NM_166201.3	NP_725626.1	protein C kinase 53E, isoform B
NM_057334.3	NP_476682.1	protein C kinase 53E, isoform A
NM_001103880.3	NP_001097350.1	protein C kinase 53E, isoform C
NM_001259449.1	NP_001246378.1	protein C kinase 53E, isoform E
NM_001274106.1	NP_001261035.1	protein C kinase 53E, isoform F

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prkca	NP_035231.2
Rattus norvegicus (Norway rat)	Prkca	NP_001099183.1
Drosophila melanogaster (fruit fly)	Pkc53E	NP_725626.1
Homo sapiens (human)	PRKCA	NP_002728.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012252	XP_320290.4
Canis lupus familiaris (dog)	PRKCA	XP_005624328.1
Bos taurus (cattle)	PRKCA	NP_776860.1
Gallus gallus (chicken)	PRKCA	NP_001012822.1
Danio rerio (zebrafish)	prkca	NP_001243170.1
	PRKCA	XP_002800615.1
Gallus gallus (chicken)	LOC101749801	XP_004946286.1
Caenorhabditis elegans (roundworm)	pkc-2	NP_001024518.1
Pan troglodytes (chimpanzee)	PRKCA	XP_003315734.1
Xenopus tropicalis (tropical clawed frog)	prkca	NP_001072752.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pkc53E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkc53E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12847	NM_001259448.1 Latest version!	Drosophila melanogaster protein C kinase 53E, transcript variant D (Pkc53E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11453	NM_166201.3 Latest version!	Drosophila melanogaster protein C kinase 53E, transcript variant B (Pkc53E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11454	NM_001103880.3 Latest version!	Drosophila melanogaster protein C kinase 53E, transcript variant C (Pkc53E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11454	NM_001259449.1 Latest version!	Drosophila melanogaster protein C kinase 53E, transcript variant E (Pkc53E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11455	NM_057334.3 Latest version!	Drosophila melanogaster protein C kinase 53E, transcript variant A (Pkc53E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11455	NM_001274106.1 Latest version!	Drosophila melanogaster protein C kinase 53E, transcript variant F (Pkc53E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkc53E ( NM_001259448.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246377.1 Drosophila melanogaster protein C kinase 53E, transcript variant D (Pkc53E), mRNA.

Price & Availability↑

CloneID	OFa12847
Clone ID Related Accession (Same CDS sequence)	NM_001259448.1
Accession Version	NM_001259448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein C kinase 53E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGTGC ATGCCCGCTG CAAGGAGAAC GTGCCCAGCC TTTGCGGATG CGATCACACG 
GAGCGGCGGG GTCGCATTTA TCTGGAGATC AATGTCAAGG AGAATCTGCT AACTGTCCAG 
ATCAAGGAGG GTCGCAATCT CATACCCATG GACCCAAACG GACTGAGCGA TCCCTATGTG 
AAGGTGAAGC TAATTCCAGA TGACAAGGAT CAATCCAAGA AGAAGACTCG CACCATTAAG 
GCTTGCCTAA ATCCTGTCTG GAATGAGACA CTCACTTATG ATCTAAAGCC CGAAGACAAG 
GATCGACGCA TCCTGATCGA AGTGTGGGAC TGGGATCGCA CTTCACGCAA TGACTTTATG 
GGTGCTTTGT CCTTCGGAAT CTCAGAGATC ATCAAGAACC CAACCAATGG CTGGTTCAAA 
CTGCTCACCC AGGACGAAGG CGAGTACTAC AATGTGCCAT GTGCGGATGA TGAGCAGGAC 
CTACTGAAGC TCAAGCAGAA ACCATCGCAA AAGAAGCCGA TGGTAATGCG CAGCGATACG 
AACACGCATA CTTCTTCCAA AAAGGATATG ATCCGGGCCA CGGACTTTAA TTTCATCAAA 
GTTCTCGGCA AGGGCTCATT TGGAAAGGTT TTGCTGGCTG AGCGCAAAGG CAGCGAGGAG 
TTGTATGCCA TTAAGATACT CAAAAAGGAT GTGATCATTC AGGACGACGA CGTCGAGTGC 
ACCATGATCG AAAAGCGTGT CCTGGCGCTG GGCGAAAAGC CACCTTTCCT GGTCCAATTA 
CACTCCTGCT TTCAGACGAT GGACCGTTTG TTCTTTGTAA TGGAGTATGT GAATGGCGGC 
GATTTGATGT TCCAAATCCA ACAGTTTGGC AAGTTTAAGG AACCAGTGGC CGTATTTTAT 
GCCGCTGAAA TAGCAGCAGG TCTTTTCTTC CTTCATACCA AGGGCATACT GTATCGAGAT 
CTAAAGCTGG ATAATGTGCT CCTAGACGCC GATGGACATG TGAAGATTGC GGACTTTGGC 
ATGTGCAAGG AGAACATAGT GGGTGATAAG ACCACAAAGA CTTTCTGCGG TACACCTGAT 
TACATTGCTC CAGAGATAAT ACTGTATCAG CCCTATGGCA AATCGGTGGA CTGGTGGGCC 
TATGGAGTAC TGCTTTATGA GATGTTAGTG GGCCAGCCAC CTTTCGATGG CGAAGATGAG 
GAGGAGCTGT TTGCCGCCAT AACTGATCAT AATGTGTCCT ATCCAAAGAG CCTGAGCAAA 
GAGGCCAAAG AGGCCTGCAA GGGCTTCCTA ACTAAGCAGC CAAATAAGCG TTTGGGTTGT 
GGTTCCTCTG GCGAGGAGGA TGTGCGTCTG CATCCCTTTT TCAGACGCAT CGATTGGGAG 
AAAATCGAAA ATCGCGAAGT GCAACCGCCA TTTAAGCCGA AAATTAAACA CCGCAAGGAT 
GTGTCCAACT TTGACAAGCA GTTCACATCA GAGAAAACAG ACTTGACGCC CACGGACAAG 
GTGTTTATGA TGAATCTGGA TCAGTCGGAA TTCGTTGGCT TCTCCTACAT GAATCCCGAA 
TATGTTTTCA GCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246377.1
CDS	166..1743
Translation	MNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEYVFSP

Target ORF information:

RefSeq Version	NM_001259448.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein C kinase 53E, transcript variant D (Pkc53E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259448.1

ORF Insert Sequence:

ATGAACGTGC ATGCCCGCTG CAAGGAGAAC GTGCCCAGCC TTTGCGGATG CGATCACACG 
GAGCGGCGGG GTCGCATTTA TCTGGAGATC AATGTCAAGG AGAATCTGCT AACTGTCCAG 
ATCAAGGAGG GTCGCAATCT CATACCCATG GACCCAAACG GACTGAGCGA TCCCTATGTG 
AAGGTGAAGC TAATTCCAGA TGACAAGGAT CAATCCAAGA AGAAGACTCG CACCATTAAG 
GCTTGCCTAA ATCCTGTCTG GAATGAGACA CTCACTTATG ATCTAAAGCC CGAAGACAAG 
GATCGACGCA TCCTGATCGA AGTGTGGGAC TGGGATCGCA CTTCACGCAA TGACTTTATG 
GGTGCTTTGT CCTTCGGAAT CTCAGAGATC ATCAAGAACC CAACCAATGG CTGGTTCAAA 
CTGCTCACCC AGGACGAAGG CGAGTACTAC AATGTGCCAT GTGCGGATGA TGAGCAGGAC 
CTACTGAAGC TCAAGCAGAA ACCATCGCAA AAGAAGCCGA TGGTAATGCG CAGCGATACG 
AACACGCATA CTTCTTCCAA AAAGGATATG ATCCGGGCCA CGGACTTTAA TTTCATCAAA 
GTTCTCGGCA AGGGCTCATT TGGAAAGGTT TTGCTGGCTG AGCGCAAAGG CAGCGAGGAG 
TTGTATGCCA TTAAGATACT CAAAAAGGAT GTGATCATTC AGGACGACGA CGTCGAGTGC 
ACCATGATCG AAAAGCGTGT CCTGGCGCTG GGCGAAAAGC CACCTTTCCT GGTCCAATTA 
CACTCCTGCT TTCAGACGAT GGACCGTTTG TTCTTTGTAA TGGAGTATGT GAATGGCGGC 
GATTTGATGT TCCAAATCCA ACAGTTTGGC AAGTTTAAGG AACCAGTGGC CGTATTTTAT 
GCCGCTGAAA TAGCAGCAGG TCTTTTCTTC CTTCATACCA AGGGCATACT GTATCGAGAT 
CTAAAGCTGG ATAATGTGCT CCTAGACGCC GATGGACATG TGAAGATTGC GGACTTTGGC 
ATGTGCAAGG AGAACATAGT GGGTGATAAG ACCACAAAGA CTTTCTGCGG TACACCTGAT 
TACATTGCTC CAGAGATAAT ACTGTATCAG CCCTATGGCA AATCGGTGGA CTGGTGGGCC 
TATGGAGTAC TGCTTTATGA GATGTTAGTG GGCCAGCCAC CTTTCGATGG CGAAGATGAG 
GAGGAGCTGT TTGCCGCCAT AACTGATCAT AATGTGTCCT ATCCAAAGAG CCTGAGCAAA 
GAGGCCAAAG AGGCCTGCAA GGGCTTCCTA ACTAAGCAGC CAAATAAGCG TTTGGGTTGT 
GGTTCCTCTG GCGAGGAGGA TGTGCGTCTG CATCCCTTTT TCAGACGCAT CGATTGGGAG 
AAAATCGAAA ATCGCGAAGT GCAACCGCCA TTTAAGCCGA AAATTAAACA CCGCAAGGAT 
GTGTCCAACT TTGACAAGCA GTTCACATCA GAGAAAACAG ACTTGACGCC CACGGACAAG 
GTGTTTATGA TGAATCTGGA TCAGTCGGAA TTCGTTGGCT TCTCCTACAT GAATCCCGAA 
TATGTTTTCA GCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkc53E ( NM_166201.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725626.1 Drosophila melanogaster protein C kinase 53E, transcript variant B (Pkc53E), mRNA.

Price & Availability↑

CloneID	OFa11453
Clone ID Related Accession (Same CDS sequence)	NM_166201.3
Accession Version	NM_166201.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein C kinase 53E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166201.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG CGGCTATCAG TCGGGATATG CATGGATGGG CTTCGGAAAG 
CAAGGATTTC AGTGCCAAGT TTGCTCCTAT GTGGTGCACA AGCGGTGCCA CGAATATGTC 
ACCTTCATCT GCCCGGGCAA GGATAAGGGC ATCGATTCGG ACTCACCAAA AACTCAACAC 
AATTTCGAGC CATTCACATA CGCAGGACCC ACGTTCTGCG ATCATTGTGG GTCCCTGCTG 
TATGGCATTT ACCACCAGGG TCTCAAATGC TCAGCATGTG ACATGAACGT GCATGCCCGC 
TGCAAGGAGA ACGTGCCCAG CCTTTGCGGA TGCGATCACA CGGAGCGGCG GGGTCGCATT 
TATCTGGAGA TCAATGTCAA GGAGAATCTG CTAACTGTCC AGATCAAGGA GGGTCGCAAT 
CTCATACCCA TGGACCCAAA CGGACTGAGC GATCCCTATG TGAAGGTGAA GCTAATTCCA 
GATGACAAGG ATCAATCCAA GAAGAAGACT CGCACCATTA AGGCTTGCCT AAATCCTGTC 
TGGAATGAGA CACTCACTTA TGATCTAAAG CCCGAAGACA AGGATCGACG CATCCTGATC 
GAAGTGTGGG ACTGGGATCG CACTTCACGC AATGACTTTA TGGGTGCTTT GTCCTTCGGA 
ATCTCAGAGA TCATCAAGAA CCCAACCAAT GGCTGGTTCA AACTGCTCAC CCAGGACGAA 
GGCGAGTACT ACAATGTGCC ATGTGCGGAT GATGAGCAGG ACCTACTGAA GCTCAAGCAG 
AAACCATCGC AAAAGAAGCC GATGGTAATG CGCAGCGATA CGAACACGCA TACTTCTTCC 
AAAAAGGATA TGATCCGGGC CACGGACTTT AATTTCATCA AAGTTCTCGG CAAGGGCTCA 
TTTGGAAAGG TTTTGCTGGC TGAGCGCAAA GGCAGCGAGG AGTTGTATGC CATTAAGATA 
CTCAAAAAGG ATGTGATCAT TCAGGACGAC GACGTCGAGT GCACCATGAT CGAAAAGCGT 
GTCCTGGCGC TGGGCGAAAA GCCACCTTTC CTGGTCCAAT TACACTCCTG CTTTCAGACG 
ATGGACCGTT TGTTCTTTGT AATGGAGTAT GTGAATGGCG GCGATTTGAT GTTCCAAATC 
CAACAGTTTG GCAAGTTTAA GGAACCAGTG GCCGTATTTT ATGCCGCTGA AATAGCAGCA 
GGTCTTTTCT TCCTTCATAC CAAGGGCATA CTGTATCGAG ATCTAAAGCT GGATAATGTG 
CTCCTAGACG CCGATGGACA TGTGAAGATT GCGGACTTTG GCATGTGCAA GGAGAACATA 
GTGGGTGATA AGACCACAAA GACTTTCTGC GGTACACCTG ATTACATTGC TCCAGAGATA 
ATACTGTATC AGCCCTATGG CAAATCGGTG GACTGGTGGG CCTATGGAGT ACTGCTTTAT 
GAGATGTTAG TGGGCCAGCC ACCTTTCGAT GGCGAAGATG AGGAGGAGCT GTTTGCCGCC 
ATAACTGATC ATAATGTGTC CTATCCAAAG AGCCTGAGCA AAGAGGCCAA AGAGGCCTGC 
AAGGGCTTCC TAACTAAGCA GCCAAATAAG CGTTTGGGTT GTGGTTCCTC TGGCGAGGAG 
GATGTGCGTC TGCATCCCTT TTTCAGACGC ATCGATTGGG AGAAAATCGA AAATCGCGAA 
GTGCAACCGC CATTTAAGCC GAAAATTAAA CACCGCAAGG ATGTGTCCAA CTTTGACAAG 
CAGTTCACAT CAGAGAAAAC AGACTTGACG CCCACGGACA AGGTGTTTAT GATGAATCTG 
GATCAGTCGG AATTCGTTGG CTTCTCCTAC ATGAATCCCG AATATGTTTT CAGCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725626.1
CDS	376..2415
Translation	MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKCSACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEYVFSP

Target ORF information:

RefSeq Version	NM_166201.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein C kinase 53E, transcript variant B (Pkc53E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166201.3

ORF Insert Sequence:

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG CGGCTATCAG TCGGGATATG CATGGATGGG CTTCGGAAAG 
CAAGGATTTC AGTGCCAAGT TTGCTCCTAT GTGGTGCACA AGCGGTGCCA CGAATATGTC 
ACCTTCATCT GCCCGGGCAA GGATAAGGGC ATCGATTCGG ACTCACCAAA AACTCAACAC 
AATTTCGAGC CATTCACATA CGCAGGACCC ACGTTCTGCG ATCATTGTGG GTCCCTGCTG 
TATGGCATTT ACCACCAGGG TCTCAAATGC TCAGCATGTG ACATGAACGT GCATGCCCGC 
TGCAAGGAGA ACGTGCCCAG CCTTTGCGGA TGCGATCACA CGGAGCGGCG GGGTCGCATT 
TATCTGGAGA TCAATGTCAA GGAGAATCTG CTAACTGTCC AGATCAAGGA GGGTCGCAAT 
CTCATACCCA TGGACCCAAA CGGACTGAGC GATCCCTATG TGAAGGTGAA GCTAATTCCA 
GATGACAAGG ATCAATCCAA GAAGAAGACT CGCACCATTA AGGCTTGCCT AAATCCTGTC 
TGGAATGAGA CACTCACTTA TGATCTAAAG CCCGAAGACA AGGATCGACG CATCCTGATC 
GAAGTGTGGG ACTGGGATCG CACTTCACGC AATGACTTTA TGGGTGCTTT GTCCTTCGGA 
ATCTCAGAGA TCATCAAGAA CCCAACCAAT GGCTGGTTCA AACTGCTCAC CCAGGACGAA 
GGCGAGTACT ACAATGTGCC ATGTGCGGAT GATGAGCAGG ACCTACTGAA GCTCAAGCAG 
AAACCATCGC AAAAGAAGCC GATGGTAATG CGCAGCGATA CGAACACGCA TACTTCTTCC 
AAAAAGGATA TGATCCGGGC CACGGACTTT AATTTCATCA AAGTTCTCGG CAAGGGCTCA 
TTTGGAAAGG TTTTGCTGGC TGAGCGCAAA GGCAGCGAGG AGTTGTATGC CATTAAGATA 
CTCAAAAAGG ATGTGATCAT TCAGGACGAC GACGTCGAGT GCACCATGAT CGAAAAGCGT 
GTCCTGGCGC TGGGCGAAAA GCCACCTTTC CTGGTCCAAT TACACTCCTG CTTTCAGACG 
ATGGACCGTT TGTTCTTTGT AATGGAGTAT GTGAATGGCG GCGATTTGAT GTTCCAAATC 
CAACAGTTTG GCAAGTTTAA GGAACCAGTG GCCGTATTTT ATGCCGCTGA AATAGCAGCA 
GGTCTTTTCT TCCTTCATAC CAAGGGCATA CTGTATCGAG ATCTAAAGCT GGATAATGTG 
CTCCTAGACG CCGATGGACA TGTGAAGATT GCGGACTTTG GCATGTGCAA GGAGAACATA 
GTGGGTGATA AGACCACAAA GACTTTCTGC GGTACACCTG ATTACATTGC TCCAGAGATA 
ATACTGTATC AGCCCTATGG CAAATCGGTG GACTGGTGGG CCTATGGAGT ACTGCTTTAT 
GAGATGTTAG TGGGCCAGCC ACCTTTCGAT GGCGAAGATG AGGAGGAGCT GTTTGCCGCC 
ATAACTGATC ATAATGTGTC CTATCCAAAG AGCCTGAGCA AAGAGGCCAA AGAGGCCTGC 
AAGGGCTTCC TAACTAAGCA GCCAAATAAG CGTTTGGGTT GTGGTTCCTC TGGCGAGGAG 
GATGTGCGTC TGCATCCCTT TTTCAGACGC ATCGATTGGG AGAAAATCGA AAATCGCGAA 
GTGCAACCGC CATTTAAGCC GAAAATTAAA CACCGCAAGG ATGTGTCCAA CTTTGACAAG 
CAGTTCACAT CAGAGAAAAC AGACTTGACG CCCACGGACA AGGTGTTTAT GATGAATCTG 
GATCAGTCGG AATTCGTTGG CTTCTCCTAC ATGAATCCCG AATATGTTTT CAGCCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkc53E ( NM_001103880.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097350.1 Drosophila melanogaster protein C kinase 53E, transcript variant C (Pkc53E), mRNA.

Price & Availability↑

CloneID	OFa11454
Clone ID Related Accession (Same CDS sequence)	NM_001103880.3 , NM_001259449.1
Accession Version	NM_001103880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein C kinase 53E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103880.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG CGGCTATCAG TCGGGATATG CATGGATGGG CTTCGGAAAG 
CAAGGATTTC AGTGCCAAGT TTGCTCCTAT GTGGTGCACA AGCGGTGCCA CGAATATGTC 
ACCTTCATCT GCCCGGGCAA GGATAAGGGC ATCGATTCGG CGCATCAAAT GCCCCACAAA 
TGGCAGAATG TCACCTTCCT GTCGCCGACG TTCTGCGATC ATTGTGGGTC CCTTTTGCAC 
GGCATCACGC ACAAGGGTCT CAAGTGTCGA GCATGTGACA TGAACGTGCA TGCCCGCTGC 
AAGGAGAACG TGCCCAGCCT TTGCGGATGC GATCACACGG AGCGGCGGGG TCGCATTTAT 
CTGGAGATCA ATGTCAAGGA GAATCTGCTA ACTGTCCAGA TCAAGGAGGG TCGCAATCTC 
ATACCCATGG ACCCAAACGG ACTGAGCGAT CCCTATGTGA AGGTGAAGCT AATTCCAGAT 
GACAAGGATC AATCCAAGAA GAAGACTCGC ACCATTAAGG CTTGCCTAAA TCCTGTCTGG 
AATGAGACAC TCACTTATGA TCTAAAGCCC GAAGACAAGG ATCGACGCAT CCTGATCGAA 
GTGTGGGACT GGGATCGCAC TTCACGCAAT GACTTTATGG GTGCTTTGTC CTTCGGAATC 
TCAGAGATCA TCAAGAACCC AACCAATGGC TGGTTCAAAC TGCTCACCCA GGACGAAGGC 
GAGTACTACA ATGTGCCATG TGCGGATGAT GAGCAGGACC TACTGAAGCT CAAGCAGAAA 
CCATCGCAAA AGAAGCCGAT GGTAATGCGC AGCGATACGA ACACGCATAC TTCTTCCAAA 
AAGGATATGA TCCGGGCCAC GGACTTTAAT TTCATCAAAG TTCTCGGCAA GGGCTCATTT 
GGAAAGGTTT TGCTGGCTGA GCGCAAAGGC AGCGAGGAGT TGTATGCCAT TAAGATACTC 
AAAAAGGATG TGATCATTCA GGACGACGAC GTCGAGTGCA CCATGATCGA AAAGCGTGTC 
CTGGCGCTGG GCGAAAAGCC ACCTTTCCTG GTCCAATTAC ACTCCTGCTT TCAGACGATG 
GACCGTTTGT TCTTTGTAAT GGAGTATGTG AATGGCGGCG ATTTGATGTT CCAAATCCAA 
CAGTTTGGCA AGTTTAAGGA ACCAGTGGCC GTATTTTATG CCGCTGAAAT AGCAGCAGGT 
CTTTTCTTCC TTCATACCAA GGGCATACTG TATCGAGATC TAAAGCTGGA TAATGTGCTC 
CTAGACGCCG ATGGACATGT GAAGATTGCG GACTTTGGCA TGTGCAAGGA GAACATAGTG 
GGTGATAAGA CCACAAAGAC TTTCTGCGGT ACACCTGATT ACATTGCTCC AGAGATAATA 
CTGTATCAGC CCTATGGCAA ATCGGTGGAC TGGTGGGCCT ATGGAGTACT GCTTTATGAG 
ATGTTAGTGG GCCAGCCACC TTTCGATGGC GAAGATGAGG AGGAGCTGTT TGCCGCCATA 
ACTGATCATA ATGTGTCCTA TCCAAAGAGC CTGAGCAAAG AGGCCAAAGA GGCCTGCAAG 
GGCTTCCTAA CTAAGCAGCC AAATAAGCGT TTGGGTTGTG GTTCCTCTGG CGAGGAGGAT 
GTGCGTCTGC ATCCCTTTTT CAGACGCATC GATTGGGAGA AAATCGAAAA TCGCGAAGTG 
CAACCGCCAT TTAAGCCGAA AATTAAACAC CGCAAGGATG TGTCCAACTT TGACAAGCAG 
TTCACATCAG AGAAAACAGA CTTGACGCCC ACGGACAAGG TGTTTATGAT GAATCTGGAT 
CAGTCGGAAT TCGTTGGCTT CTCCTACATG AATCCCGAAT ATGTTTTCAG CCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097350.1
CDS	376..2412
Translation	MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSAHQMPHKWQNVTFLSPTFCDHCGSLLHGITHKGLKCRACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEYVFSP

Target ORF information:

RefSeq Version	NM_001103880.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein C kinase 53E, transcript variant C (Pkc53E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103880.3

ORF Insert Sequence:

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG CGGCTATCAG TCGGGATATG CATGGATGGG CTTCGGAAAG 
CAAGGATTTC AGTGCCAAGT TTGCTCCTAT GTGGTGCACA AGCGGTGCCA CGAATATGTC 
ACCTTCATCT GCCCGGGCAA GGATAAGGGC ATCGATTCGG CGCATCAAAT GCCCCACAAA 
TGGCAGAATG TCACCTTCCT GTCGCCGACG TTCTGCGATC ATTGTGGGTC CCTTTTGCAC 
GGCATCACGC ACAAGGGTCT CAAGTGTCGA GCATGTGACA TGAACGTGCA TGCCCGCTGC 
AAGGAGAACG TGCCCAGCCT TTGCGGATGC GATCACACGG AGCGGCGGGG TCGCATTTAT 
CTGGAGATCA ATGTCAAGGA GAATCTGCTA ACTGTCCAGA TCAAGGAGGG TCGCAATCTC 
ATACCCATGG ACCCAAACGG ACTGAGCGAT CCCTATGTGA AGGTGAAGCT AATTCCAGAT 
GACAAGGATC AATCCAAGAA GAAGACTCGC ACCATTAAGG CTTGCCTAAA TCCTGTCTGG 
AATGAGACAC TCACTTATGA TCTAAAGCCC GAAGACAAGG ATCGACGCAT CCTGATCGAA 
GTGTGGGACT GGGATCGCAC TTCACGCAAT GACTTTATGG GTGCTTTGTC CTTCGGAATC 
TCAGAGATCA TCAAGAACCC AACCAATGGC TGGTTCAAAC TGCTCACCCA GGACGAAGGC 
GAGTACTACA ATGTGCCATG TGCGGATGAT GAGCAGGACC TACTGAAGCT CAAGCAGAAA 
CCATCGCAAA AGAAGCCGAT GGTAATGCGC AGCGATACGA ACACGCATAC TTCTTCCAAA 
AAGGATATGA TCCGGGCCAC GGACTTTAAT TTCATCAAAG TTCTCGGCAA GGGCTCATTT 
GGAAAGGTTT TGCTGGCTGA GCGCAAAGGC AGCGAGGAGT TGTATGCCAT TAAGATACTC 
AAAAAGGATG TGATCATTCA GGACGACGAC GTCGAGTGCA CCATGATCGA AAAGCGTGTC 
CTGGCGCTGG GCGAAAAGCC ACCTTTCCTG GTCCAATTAC ACTCCTGCTT TCAGACGATG 
GACCGTTTGT TCTTTGTAAT GGAGTATGTG AATGGCGGCG ATTTGATGTT CCAAATCCAA 
CAGTTTGGCA AGTTTAAGGA ACCAGTGGCC GTATTTTATG CCGCTGAAAT AGCAGCAGGT 
CTTTTCTTCC TTCATACCAA GGGCATACTG TATCGAGATC TAAAGCTGGA TAATGTGCTC 
CTAGACGCCG ATGGACATGT GAAGATTGCG GACTTTGGCA TGTGCAAGGA GAACATAGTG 
GGTGATAAGA CCACAAAGAC TTTCTGCGGT ACACCTGATT ACATTGCTCC AGAGATAATA 
CTGTATCAGC CCTATGGCAA ATCGGTGGAC TGGTGGGCCT ATGGAGTACT GCTTTATGAG 
ATGTTAGTGG GCCAGCCACC TTTCGATGGC GAAGATGAGG AGGAGCTGTT TGCCGCCATA 
ACTGATCATA ATGTGTCCTA TCCAAAGAGC CTGAGCAAAG AGGCCAAAGA GGCCTGCAAG 
GGCTTCCTAA CTAAGCAGCC AAATAAGCGT TTGGGTTGTG GTTCCTCTGG CGAGGAGGAT 
GTGCGTCTGC ATCCCTTTTT CAGACGCATC GATTGGGAGA AAATCGAAAA TCGCGAAGTG 
CAACCGCCAT TTAAGCCGAA AATTAAACAC CGCAAGGATG TGTCCAACTT TGACAAGCAG 
TTCACATCAG AGAAAACAGA CTTGACGCCC ACGGACAAGG TGTTTATGAT GAATCTGGAT 
CAGTCGGAAT TCGTTGGCTT CTCCTACATG AATCCCGAAT ATGTTTTCAG CCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkc53E ( NM_001259449.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246378.1 Drosophila melanogaster protein C kinase 53E, transcript variant E (Pkc53E), mRNA.

Price & Availability↑

CloneID	OFa11454
Clone ID Related Accession (Same CDS sequence)	NM_001103880.3 , NM_001259449.1
Accession Version	NM_001259449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein C kinase 53E, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG CGGCTATCAG TCGGGATATG CATGGATGGG CTTCGGAAAG 
CAAGGATTTC AGTGCCAAGT TTGCTCCTAT GTGGTGCACA AGCGGTGCCA CGAATATGTC 
ACCTTCATCT GCCCGGGCAA GGATAAGGGC ATCGATTCGG CGCATCAAAT GCCCCACAAA 
TGGCAGAATG TCACCTTCCT GTCGCCGACG TTCTGCGATC ATTGTGGGTC CCTTTTGCAC 
GGCATCACGC ACAAGGGTCT CAAGTGTCGA GCATGTGACA TGAACGTGCA TGCCCGCTGC 
AAGGAGAACG TGCCCAGCCT TTGCGGATGC GATCACACGG AGCGGCGGGG TCGCATTTAT 
CTGGAGATCA ATGTCAAGGA GAATCTGCTA ACTGTCCAGA TCAAGGAGGG TCGCAATCTC 
ATACCCATGG ACCCAAACGG ACTGAGCGAT CCCTATGTGA AGGTGAAGCT AATTCCAGAT 
GACAAGGATC AATCCAAGAA GAAGACTCGC ACCATTAAGG CTTGCCTAAA TCCTGTCTGG 
AATGAGACAC TCACTTATGA TCTAAAGCCC GAAGACAAGG ATCGACGCAT CCTGATCGAA 
GTGTGGGACT GGGATCGCAC TTCACGCAAT GACTTTATGG GTGCTTTGTC CTTCGGAATC 
TCAGAGATCA TCAAGAACCC AACCAATGGC TGGTTCAAAC TGCTCACCCA GGACGAAGGC 
GAGTACTACA ATGTGCCATG TGCGGATGAT GAGCAGGACC TACTGAAGCT CAAGCAGAAA 
CCATCGCAAA AGAAGCCGAT GGTAATGCGC AGCGATACGA ACACGCATAC TTCTTCCAAA 
AAGGATATGA TCCGGGCCAC GGACTTTAAT TTCATCAAAG TTCTCGGCAA GGGCTCATTT 
GGAAAGGTTT TGCTGGCTGA GCGCAAAGGC AGCGAGGAGT TGTATGCCAT TAAGATACTC 
AAAAAGGATG TGATCATTCA GGACGACGAC GTCGAGTGCA CCATGATCGA AAAGCGTGTC 
CTGGCGCTGG GCGAAAAGCC ACCTTTCCTG GTCCAATTAC ACTCCTGCTT TCAGACGATG 
GACCGTTTGT TCTTTGTAAT GGAGTATGTG AATGGCGGCG ATTTGATGTT CCAAATCCAA 
CAGTTTGGCA AGTTTAAGGA ACCAGTGGCC GTATTTTATG CCGCTGAAAT AGCAGCAGGT 
CTTTTCTTCC TTCATACCAA GGGCATACTG TATCGAGATC TAAAGCTGGA TAATGTGCTC 
CTAGACGCCG ATGGACATGT GAAGATTGCG GACTTTGGCA TGTGCAAGGA GAACATAGTG 
GGTGATAAGA CCACAAAGAC TTTCTGCGGT ACACCTGATT ACATTGCTCC AGAGATAATA 
CTGTATCAGC CCTATGGCAA ATCGGTGGAC TGGTGGGCCT ATGGAGTACT GCTTTATGAG 
ATGTTAGTGG GCCAGCCACC TTTCGATGGC GAAGATGAGG AGGAGCTGTT TGCCGCCATA 
ACTGATCATA ATGTGTCCTA TCCAAAGAGC CTGAGCAAAG AGGCCAAAGA GGCCTGCAAG 
GGCTTCCTAA CTAAGCAGCC AAATAAGCGT TTGGGTTGTG GTTCCTCTGG CGAGGAGGAT 
GTGCGTCTGC ATCCCTTTTT CAGACGCATC GATTGGGAGA AAATCGAAAA TCGCGAAGTG 
CAACCGCCAT TTAAGCCGAA AATTAAACAC CGCAAGGATG TGTCCAACTT TGACAAGCAG 
TTCACATCAG AGAAAACAGA CTTGACGCCC ACGGACAAGG TGTTTATGAT GAATCTGGAT 
CAGTCGGAAT TCGTTGGCTT CTCCTACATG AATCCCGAAT ATGTTTTCAG CCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246378.1
CDS	316..2352
Translation	MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFICGYQSGYAWMGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSAHQMPHKWQNVTFLSPTFCDHCGSLLHGITHKGLKCRACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEYVFSP

Target ORF information:

RefSeq Version	NM_001259449.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein C kinase 53E, transcript variant E (Pkc53E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259449.1

ORF Insert Sequence:

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG CGGCTATCAG TCGGGATATG CATGGATGGG CTTCGGAAAG 
CAAGGATTTC AGTGCCAAGT TTGCTCCTAT GTGGTGCACA AGCGGTGCCA CGAATATGTC 
ACCTTCATCT GCCCGGGCAA GGATAAGGGC ATCGATTCGG CGCATCAAAT GCCCCACAAA 
TGGCAGAATG TCACCTTCCT GTCGCCGACG TTCTGCGATC ATTGTGGGTC CCTTTTGCAC 
GGCATCACGC ACAAGGGTCT CAAGTGTCGA GCATGTGACA TGAACGTGCA TGCCCGCTGC 
AAGGAGAACG TGCCCAGCCT TTGCGGATGC GATCACACGG AGCGGCGGGG TCGCATTTAT 
CTGGAGATCA ATGTCAAGGA GAATCTGCTA ACTGTCCAGA TCAAGGAGGG TCGCAATCTC 
ATACCCATGG ACCCAAACGG ACTGAGCGAT CCCTATGTGA AGGTGAAGCT AATTCCAGAT 
GACAAGGATC AATCCAAGAA GAAGACTCGC ACCATTAAGG CTTGCCTAAA TCCTGTCTGG 
AATGAGACAC TCACTTATGA TCTAAAGCCC GAAGACAAGG ATCGACGCAT CCTGATCGAA 
GTGTGGGACT GGGATCGCAC TTCACGCAAT GACTTTATGG GTGCTTTGTC CTTCGGAATC 
TCAGAGATCA TCAAGAACCC AACCAATGGC TGGTTCAAAC TGCTCACCCA GGACGAAGGC 
GAGTACTACA ATGTGCCATG TGCGGATGAT GAGCAGGACC TACTGAAGCT CAAGCAGAAA 
CCATCGCAAA AGAAGCCGAT GGTAATGCGC AGCGATACGA ACACGCATAC TTCTTCCAAA 
AAGGATATGA TCCGGGCCAC GGACTTTAAT TTCATCAAAG TTCTCGGCAA GGGCTCATTT 
GGAAAGGTTT TGCTGGCTGA GCGCAAAGGC AGCGAGGAGT TGTATGCCAT TAAGATACTC 
AAAAAGGATG TGATCATTCA GGACGACGAC GTCGAGTGCA CCATGATCGA AAAGCGTGTC 
CTGGCGCTGG GCGAAAAGCC ACCTTTCCTG GTCCAATTAC ACTCCTGCTT TCAGACGATG 
GACCGTTTGT TCTTTGTAAT GGAGTATGTG AATGGCGGCG ATTTGATGTT CCAAATCCAA 
CAGTTTGGCA AGTTTAAGGA ACCAGTGGCC GTATTTTATG CCGCTGAAAT AGCAGCAGGT 
CTTTTCTTCC TTCATACCAA GGGCATACTG TATCGAGATC TAAAGCTGGA TAATGTGCTC 
CTAGACGCCG ATGGACATGT GAAGATTGCG GACTTTGGCA TGTGCAAGGA GAACATAGTG 
GGTGATAAGA CCACAAAGAC TTTCTGCGGT ACACCTGATT ACATTGCTCC AGAGATAATA 
CTGTATCAGC CCTATGGCAA ATCGGTGGAC TGGTGGGCCT ATGGAGTACT GCTTTATGAG 
ATGTTAGTGG GCCAGCCACC TTTCGATGGC GAAGATGAGG AGGAGCTGTT TGCCGCCATA 
ACTGATCATA ATGTGTCCTA TCCAAAGAGC CTGAGCAAAG AGGCCAAAGA GGCCTGCAAG 
GGCTTCCTAA CTAAGCAGCC AAATAAGCGT TTGGGTTGTG GTTCCTCTGG CGAGGAGGAT 
GTGCGTCTGC ATCCCTTTTT CAGACGCATC GATTGGGAGA AAATCGAAAA TCGCGAAGTG 
CAACCGCCAT TTAAGCCGAA AATTAAACAC CGCAAGGATG TGTCCAACTT TGACAAGCAG 
TTCACATCAG AGAAAACAGA CTTGACGCCC ACGGACAAGG TGTTTATGAT GAATCTGGAT 
CAGTCGGAAT TCGTTGGCTT CTCCTACATG AATCCCGAAT ATGTTTTCAG CCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkc53E ( NM_057334.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476682.1 Drosophila melanogaster protein C kinase 53E, transcript variant A (Pkc53E), mRNA.

Price & Availability↑

CloneID	OFa11455
Clone ID Related Accession (Same CDS sequence)	NM_057334.3 , NM_001274106.1
Accession Version	NM_057334.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein C kinase 53E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057334.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG GGGCTTCGGA AAGCAAGGAT TTCAGTGCCA AGTTTGCTCC 
TATGTGGTGC ACAAGCGGTG CCACGAATAT GTCACCTTCA TCTGCCCGGG CAAGGATAAG 
GGCATCGATT CGGACTCACC AAAAACTCAA CACAATTTCG AGCCATTCAC ATACGCAGGA 
CCCACGTTCT GCGATCATTG TGGGTCCCTG CTGTATGGCA TTTACCACCA GGGTCTCAAA 
TGCTCAGCAT GTGACATGAA CGTGCATGCC CGCTGCAAGG AGAACGTGCC CAGCCTTTGC 
GGATGCGATC ACACGGAGCG GCGGGGTCGC ATTTATCTGG AGATCAATGT CAAGGAGAAT 
CTGCTAACTG TCCAGATCAA GGAGGGTCGC AATCTCATAC CCATGGACCC AAACGGACTG 
AGCGATCCCT ATGTGAAGGT GAAGCTAATT CCAGATGACA AGGATCAATC CAAGAAGAAG 
ACTCGCACCA TTAAGGCTTG CCTAAATCCT GTCTGGAATG AGACACTCAC TTATGATCTA 
AAGCCCGAAG ACAAGGATCG ACGCATCCTG ATCGAAGTGT GGGACTGGGA TCGCACTTCA 
CGCAATGACT TTATGGGTGC TTTGTCCTTC GGAATCTCAG AGATCATCAA GAACCCAACC 
AATGGCTGGT TCAAACTGCT CACCCAGGAC GAAGGCGAGT ACTACAATGT GCCATGTGCG 
GATGATGAGC AGGACCTACT GAAGCTCAAG CAGAAACCAT CGCAAAAGAA GCCGATGGTA 
ATGCGCAGCG ATACGAACAC GCATACTTCT TCCAAAAAGG ATATGATCCG GGCCACGGAC 
TTTAATTTCA TCAAAGTTCT CGGCAAGGGC TCATTTGGAA AGGTTTTGCT GGCTGAGCGC 
AAAGGCAGCG AGGAGTTGTA TGCCATTAAG ATACTCAAAA AGGATGTGAT CATTCAGGAC 
GACGACGTCG AGTGCACCAT GATCGAAAAG CGTGTCCTGG CGCTGGGCGA AAAGCCACCT 
TTCCTGGTCC AATTACACTC CTGCTTTCAG ACGATGGACC GTTTGTTCTT TGTAATGGAG 
TATGTGAATG GCGGCGATTT GATGTTCCAA ATCCAACAGT TTGGCAAGTT TAAGGAACCA 
GTGGCCGTAT TTTATGCCGC TGAAATAGCA GCAGGTCTTT TCTTCCTTCA TACCAAGGGC 
ATACTGTATC GAGATCTAAA GCTGGATAAT GTGCTCCTAG ACGCCGATGG ACATGTGAAG 
ATTGCGGACT TTGGCATGTG CAAGGAGAAC ATAGTGGGTG ATAAGACCAC AAAGACTTTC 
TGCGGTACAC CTGATTACAT TGCTCCAGAG ATAATACTGT ATCAGCCCTA TGGCAAATCG 
GTGGACTGGT GGGCCTATGG AGTACTGCTT TATGAGATGT TAGTGGGCCA GCCACCTTTC 
GATGGCGAAG ATGAGGAGGA GCTGTTTGCC GCCATAACTG ATCATAATGT GTCCTATCCA 
AAGAGCCTGA GCAAAGAGGC CAAAGAGGCC TGCAAGGGCT TCCTAACTAA GCAGCCAAAT 
AAGCGTTTGG GTTGTGGTTC CTCTGGCGAG GAGGATGTGC GTCTGCATCC CTTTTTCAGA 
CGCATCGATT GGGAGAAAAT CGAAAATCGC GAAGTGCAAC CGCCATTTAA GCCGAAAATT 
AAACACCGCA AGGATGTGTC CAACTTTGAC AAGCAGTTCA CATCAGAGAA AACAGACTTG 
ACGCCCACGG ACAAGGTGTT TATGATGAAT CTGGATCAGT CGGAATTCGT TGGCTTCTCC 
TACATGAATC CCGAATATGT TTTCAGCCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476682.1
CDS	882..2894
Translation	MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFIWGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKCSACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEYVFSP

Target ORF information:

RefSeq Version	NM_057334.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein C kinase 53E, transcript variant A (Pkc53E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057334.3

ORF Insert Sequence:

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG GGGCTTCGGA AAGCAAGGAT TTCAGTGCCA AGTTTGCTCC 
TATGTGGTGC ACAAGCGGTG CCACGAATAT GTCACCTTCA TCTGCCCGGG CAAGGATAAG 
GGCATCGATT CGGACTCACC AAAAACTCAA CACAATTTCG AGCCATTCAC ATACGCAGGA 
CCCACGTTCT GCGATCATTG TGGGTCCCTG CTGTATGGCA TTTACCACCA GGGTCTCAAA 
TGCTCAGCAT GTGACATGAA CGTGCATGCC CGCTGCAAGG AGAACGTGCC CAGCCTTTGC 
GGATGCGATC ACACGGAGCG GCGGGGTCGC ATTTATCTGG AGATCAATGT CAAGGAGAAT 
CTGCTAACTG TCCAGATCAA GGAGGGTCGC AATCTCATAC CCATGGACCC AAACGGACTG 
AGCGATCCCT ATGTGAAGGT GAAGCTAATT CCAGATGACA AGGATCAATC CAAGAAGAAG 
ACTCGCACCA TTAAGGCTTG CCTAAATCCT GTCTGGAATG AGACACTCAC TTATGATCTA 
AAGCCCGAAG ACAAGGATCG ACGCATCCTG ATCGAAGTGT GGGACTGGGA TCGCACTTCA 
CGCAATGACT TTATGGGTGC TTTGTCCTTC GGAATCTCAG AGATCATCAA GAACCCAACC 
AATGGCTGGT TCAAACTGCT CACCCAGGAC GAAGGCGAGT ACTACAATGT GCCATGTGCG 
GATGATGAGC AGGACCTACT GAAGCTCAAG CAGAAACCAT CGCAAAAGAA GCCGATGGTA 
ATGCGCAGCG ATACGAACAC GCATACTTCT TCCAAAAAGG ATATGATCCG GGCCACGGAC 
TTTAATTTCA TCAAAGTTCT CGGCAAGGGC TCATTTGGAA AGGTTTTGCT GGCTGAGCGC 
AAAGGCAGCG AGGAGTTGTA TGCCATTAAG ATACTCAAAA AGGATGTGAT CATTCAGGAC 
GACGACGTCG AGTGCACCAT GATCGAAAAG CGTGTCCTGG CGCTGGGCGA AAAGCCACCT 
TTCCTGGTCC AATTACACTC CTGCTTTCAG ACGATGGACC GTTTGTTCTT TGTAATGGAG 
TATGTGAATG GCGGCGATTT GATGTTCCAA ATCCAACAGT TTGGCAAGTT TAAGGAACCA 
GTGGCCGTAT TTTATGCCGC TGAAATAGCA GCAGGTCTTT TCTTCCTTCA TACCAAGGGC 
ATACTGTATC GAGATCTAAA GCTGGATAAT GTGCTCCTAG ACGCCGATGG ACATGTGAAG 
ATTGCGGACT TTGGCATGTG CAAGGAGAAC ATAGTGGGTG ATAAGACCAC AAAGACTTTC 
TGCGGTACAC CTGATTACAT TGCTCCAGAG ATAATACTGT ATCAGCCCTA TGGCAAATCG 
GTGGACTGGT GGGCCTATGG AGTACTGCTT TATGAGATGT TAGTGGGCCA GCCACCTTTC 
GATGGCGAAG ATGAGGAGGA GCTGTTTGCC GCCATAACTG ATCATAATGT GTCCTATCCA 
AAGAGCCTGA GCAAAGAGGC CAAAGAGGCC TGCAAGGGCT TCCTAACTAA GCAGCCAAAT 
AAGCGTTTGG GTTGTGGTTC CTCTGGCGAG GAGGATGTGC GTCTGCATCC CTTTTTCAGA 
CGCATCGATT GGGAGAAAAT CGAAAATCGC GAAGTGCAAC CGCCATTTAA GCCGAAAATT 
AAACACCGCA AGGATGTGTC CAACTTTGAC AAGCAGTTCA CATCAGAGAA AACAGACTTG 
ACGCCCACGG ACAAGGTGTT TATGATGAAT CTGGATCAGT CGGAATTCGT TGGCTTCTCC 
TACATGAATC CCGAATATGT TTTCAGCCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pkc53E ( NM_001274106.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261035.1 Drosophila melanogaster protein C kinase 53E, transcript variant F (Pkc53E), mRNA.

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CloneID	OFa11455
Clone ID Related Accession (Same CDS sequence)	NM_057334.3 , NM_001274106.1
Accession Version	NM_001274106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein C kinase 53E, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG GGGCTTCGGA AAGCAAGGAT TTCAGTGCCA AGTTTGCTCC 
TATGTGGTGC ACAAGCGGTG CCACGAATAT GTCACCTTCA TCTGCCCGGG CAAGGATAAG 
GGCATCGATT CGGACTCACC AAAAACTCAA CACAATTTCG AGCCATTCAC ATACGCAGGA 
CCCACGTTCT GCGATCATTG TGGGTCCCTG CTGTATGGCA TTTACCACCA GGGTCTCAAA 
TGCTCAGCAT GTGACATGAA CGTGCATGCC CGCTGCAAGG AGAACGTGCC CAGCCTTTGC 
GGATGCGATC ACACGGAGCG GCGGGGTCGC ATTTATCTGG AGATCAATGT CAAGGAGAAT 
CTGCTAACTG TCCAGATCAA GGAGGGTCGC AATCTCATAC CCATGGACCC AAACGGACTG 
AGCGATCCCT ATGTGAAGGT GAAGCTAATT CCAGATGACA AGGATCAATC CAAGAAGAAG 
ACTCGCACCA TTAAGGCTTG CCTAAATCCT GTCTGGAATG AGACACTCAC TTATGATCTA 
AAGCCCGAAG ACAAGGATCG ACGCATCCTG ATCGAAGTGT GGGACTGGGA TCGCACTTCA 
CGCAATGACT TTATGGGTGC TTTGTCCTTC GGAATCTCAG AGATCATCAA GAACCCAACC 
AATGGCTGGT TCAAACTGCT CACCCAGGAC GAAGGCGAGT ACTACAATGT GCCATGTGCG 
GATGATGAGC AGGACCTACT GAAGCTCAAG CAGAAACCAT CGCAAAAGAA GCCGATGGTA 
ATGCGCAGCG ATACGAACAC GCATACTTCT TCCAAAAAGG ATATGATCCG GGCCACGGAC 
TTTAATTTCA TCAAAGTTCT CGGCAAGGGC TCATTTGGAA AGGTTTTGCT GGCTGAGCGC 
AAAGGCAGCG AGGAGTTGTA TGCCATTAAG ATACTCAAAA AGGATGTGAT CATTCAGGAC 
GACGACGTCG AGTGCACCAT GATCGAAAAG CGTGTCCTGG CGCTGGGCGA AAAGCCACCT 
TTCCTGGTCC AATTACACTC CTGCTTTCAG ACGATGGACC GTTTGTTCTT TGTAATGGAG 
TATGTGAATG GCGGCGATTT GATGTTCCAA ATCCAACAGT TTGGCAAGTT TAAGGAACCA 
GTGGCCGTAT TTTATGCCGC TGAAATAGCA GCAGGTCTTT TCTTCCTTCA TACCAAGGGC 
ATACTGTATC GAGATCTAAA GCTGGATAAT GTGCTCCTAG ACGCCGATGG ACATGTGAAG 
ATTGCGGACT TTGGCATGTG CAAGGAGAAC ATAGTGGGTG ATAAGACCAC AAAGACTTTC 
TGCGGTACAC CTGATTACAT TGCTCCAGAG ATAATACTGT ATCAGCCCTA TGGCAAATCG 
GTGGACTGGT GGGCCTATGG AGTACTGCTT TATGAGATGT TAGTGGGCCA GCCACCTTTC 
GATGGCGAAG ATGAGGAGGA GCTGTTTGCC GCCATAACTG ATCATAATGT GTCCTATCCA 
AAGAGCCTGA GCAAAGAGGC CAAAGAGGCC TGCAAGGGCT TCCTAACTAA GCAGCCAAAT 
AAGCGTTTGG GTTGTGGTTC CTCTGGCGAG GAGGATGTGC GTCTGCATCC CTTTTTCAGA 
CGCATCGATT GGGAGAAAAT CGAAAATCGC GAAGTGCAAC CGCCATTTAA GCCGAAAATT 
AAACACCGCA AGGATGTGTC CAACTTTGAC AAGCAGTTCA CATCAGAGAA AACAGACTTG 
ACGCCCACGG ACAAGGTGTT TATGATGAAT CTGGATCAGT CGGAATTCGT TGGCTTCTCC 
TACATGAATC CCGAATATGT TTTCAGCCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261035.1
CDS	399..2411
Translation	MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFIWGFGKQGFQCQVCSYVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKCSACDMNVHARCKENVPSLCGCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLKPEDKDRRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMVMRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPPFLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVKIADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYPKSLSKEAKEACKGFLTKQPNKRLGCGSSGEEDVRLHPFFRRIDWEKIENREVQPPFKPKIKHRKDVSNFDKQFTSEKTDLTPTDKVFMMNLDQSEFVGFSYMNPEYVFSP

Target ORF information:

RefSeq Version	NM_001274106.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein C kinase 53E, transcript variant F (Pkc53E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274106.1

ORF Insert Sequence:

ATGTCGGAGG GCAGCGATAA CAACGGGGAT CCCCAGCAGC AGGGGGCGGA GGGGGAGGCG 
GTGGGCGAGA ACAAAATGAA GTCCCGCCTC CGCAAAGGAG CCCTCAAGAA GAAGAACGTC 
TTCAATGTCA AGGATCACTG CTTCATCGCC CGGTTCTTCA AGCAGCCCAC GTTCTGCTCC 
CACTGCAAGG ACTTTATCTG GGGCTTCGGA AAGCAAGGAT TTCAGTGCCA AGTTTGCTCC 
TATGTGGTGC ACAAGCGGTG CCACGAATAT GTCACCTTCA TCTGCCCGGG CAAGGATAAG 
GGCATCGATT CGGACTCACC AAAAACTCAA CACAATTTCG AGCCATTCAC ATACGCAGGA 
CCCACGTTCT GCGATCATTG TGGGTCCCTG CTGTATGGCA TTTACCACCA GGGTCTCAAA 
TGCTCAGCAT GTGACATGAA CGTGCATGCC CGCTGCAAGG AGAACGTGCC CAGCCTTTGC 
GGATGCGATC ACACGGAGCG GCGGGGTCGC ATTTATCTGG AGATCAATGT CAAGGAGAAT 
CTGCTAACTG TCCAGATCAA GGAGGGTCGC AATCTCATAC CCATGGACCC AAACGGACTG 
AGCGATCCCT ATGTGAAGGT GAAGCTAATT CCAGATGACA AGGATCAATC CAAGAAGAAG 
ACTCGCACCA TTAAGGCTTG CCTAAATCCT GTCTGGAATG AGACACTCAC TTATGATCTA 
AAGCCCGAAG ACAAGGATCG ACGCATCCTG ATCGAAGTGT GGGACTGGGA TCGCACTTCA 
CGCAATGACT TTATGGGTGC TTTGTCCTTC GGAATCTCAG AGATCATCAA GAACCCAACC 
AATGGCTGGT TCAAACTGCT CACCCAGGAC GAAGGCGAGT ACTACAATGT GCCATGTGCG 
GATGATGAGC AGGACCTACT GAAGCTCAAG CAGAAACCAT CGCAAAAGAA GCCGATGGTA 
ATGCGCAGCG ATACGAACAC GCATACTTCT TCCAAAAAGG ATATGATCCG GGCCACGGAC 
TTTAATTTCA TCAAAGTTCT CGGCAAGGGC TCATTTGGAA AGGTTTTGCT GGCTGAGCGC 
AAAGGCAGCG AGGAGTTGTA TGCCATTAAG ATACTCAAAA AGGATGTGAT CATTCAGGAC 
GACGACGTCG AGTGCACCAT GATCGAAAAG CGTGTCCTGG CGCTGGGCGA AAAGCCACCT 
TTCCTGGTCC AATTACACTC CTGCTTTCAG ACGATGGACC GTTTGTTCTT TGTAATGGAG 
TATGTGAATG GCGGCGATTT GATGTTCCAA ATCCAACAGT TTGGCAAGTT TAAGGAACCA 
GTGGCCGTAT TTTATGCCGC TGAAATAGCA GCAGGTCTTT TCTTCCTTCA TACCAAGGGC 
ATACTGTATC GAGATCTAAA GCTGGATAAT GTGCTCCTAG ACGCCGATGG ACATGTGAAG 
ATTGCGGACT TTGGCATGTG CAAGGAGAAC ATAGTGGGTG ATAAGACCAC AAAGACTTTC 
TGCGGTACAC CTGATTACAT TGCTCCAGAG ATAATACTGT ATCAGCCCTA TGGCAAATCG 
GTGGACTGGT GGGCCTATGG AGTACTGCTT TATGAGATGT TAGTGGGCCA GCCACCTTTC 
GATGGCGAAG ATGAGGAGGA GCTGTTTGCC GCCATAACTG ATCATAATGT GTCCTATCCA 
AAGAGCCTGA GCAAAGAGGC CAAAGAGGCC TGCAAGGGCT TCCTAACTAA GCAGCCAAAT 
AAGCGTTTGG GTTGTGGTTC CTCTGGCGAG GAGGATGTGC GTCTGCATCC CTTTTTCAGA 
CGCATCGATT GGGAGAAAAT CGAAAATCGC GAAGTGCAAC CGCCATTTAA GCCGAAAATT 
AAACACCGCA AGGATGTGTC CAACTTTGAC AAGCAGTTCA CATCAGAGAA AACAGACTTG 
ACGCCCACGG ACAAGGTGTT TATGATGAAT CTGGATCAGT CGGAATTCGT TGGCTTCTCC 
TACATGAATC CCGAATATGT TTTCAGCCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pkc53E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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