Su(z)12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Su(z)12
Entrez Gene ID	48071
Full Name	CG8013 gene product from transcript CG8013-RB
Synonyms	CG8013,Dmel\CG8013,SU(Z)12,SUZ12,Su(Z)12,Su(z)[12],l(3)76BDo,su(z)12
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 76D4-76D4\|3-49.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_143802.3	NP_652059.1	Su(z)12, isoform A
NM_168826.2	NP_730465.1	Su(z)12, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SUZ12	XP_001174690.1
Rattus norvegicus (Norway rat)	LOC688041	XP_006247027.1
Drosophila melanogaster (fruit fly)	Su(z)12	NP_652059.1
Gallus gallus (chicken)	SUZ12	XP_415658.2
Canis lupus familiaris (dog)	SUZ12	XP_548278.2
Xenopus tropicalis (tropical clawed frog)	suz12	NP_001072292.1
Homo sapiens (human)	SUZ12	NP_056170.2
Bos taurus (cattle)	SUZ12	NP_001192516.1
Danio rerio (zebrafish)	suz12b	NP_001076293.2
Mus musculus (house mouse)	Suz12	NP_954666.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Chracterization of functionally critical SU(Z)12 elements, including key determinants of SU(Z)12-E(Z) communication.
Title: Elements of the polycomb repressor SU(Z)12 needed for histone H3-K27 methylation, the interface with E(Z), and in vivo function.

Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites.
Title: Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites.

Data show that SU(Z)12 and Heterochromatin Protein 1 (HP1) do not co-localize.
Title: In vivo analysis of Drosophila SU(Z)12 function.

A Pcl-PSC2 complex is the histone methyltransferase that generates the high levels of histone3-lysine27 trimethylation in Polycomb target genes that are needed to maintain a Polycomb-repressed chromatin state.
Title: Pcl-PRC2 is needed to generate high levels of H3-K27 trimethylation at Polycomb target genes.

The nucleosome-binding subunits Su(z)12 and Nurf55 anchor the E(z) enzyme on chromatin substrates, an essential process in maintaining HOX gene silencing during development.
Title: Nucleosome binding and histone methyltransferase activity of Drosophila PRC2.

The following Su(z)12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Su(z)12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20250	NM_168826.2 Latest version!	Drosophila melanogaster Su(z)12, transcript variant B (Su(z)12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20251	NM_143802.3 Latest version!	Drosophila melanogaster Su(z)12, transcript variant A (Su(z)12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Su(z)12 ( NM_168826.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730465.1 Drosophila melanogaster Su(z)12, transcript variant B (Su(z)12), mRNA.

Price & Availability↑

CloneID	OFa20250
Clone ID Related Accession (Same CDS sequence)	NM_168826.2
Accession Version	NM_168826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Su(z)12, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168826.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCCAG CGAAAAAACG CGAAAAAGAC AGTAATCCGG ATGGTTCTGC CGCAAATGGC 
ATTATTGGCC TCACCCATGG AGCACCGGAC GCGAGCAATG CTGGCTCTAC TGTACCGCCG 
ACGGCCGAGG GTCAGGTCAA ACTCAATGGC CACCAGCAGG AGCAGGAACT CTTTTTGCAG 
GCCTTTGAGA AGCCCACGCA GATCTATCGC TACCTGCGCA ATCGACACGA GACAAACCCC 
ATCTTCCTCA ACCGCACGCT CAGCTACATG AAGGAACGGA TGTCGCGGAA CAACAAGAAG 
CGGATCAGCT TCCAGGTAAA CTCCATGCTG GAGAGCATAA CACAAAAGTC GGAGGCGGTG 
AGCCAGAACT ACCTGCACGT CATCTACGAT TCGCTGCACG AGAAGTTGCC GGCGAGGTTG 
GACAACGAGT CTGGCGAGGA TTTGCTGCAG GAGCAGCTGC TCTGCGAAGC GGGCGAGTCT 
GTGAGTGTGG AGACAACGCT GTACAAGATC ACACGCAGCA AGCGAAAGGA CAGCACGCTG 
GACTTTCAGG AGCTGCTCAG CAAGTGCTCG CAGATCGTGT ACAACCCAAA GGATCGTGTG 
GGAGAACATG CCACCATCAG CATTCCTCTC CAGACGATGC GCCCAATGGG TGAGCAGCAC 
ACACTGTACA AGCTGCTATT TCGCATCAAG GTGCTGTCGC CAAGTACGTG CAATGATGAG 
AATGCAGAAA CTCCACCGAA CAAGCGGTCG CGTCCAAATG AGAAAATGTT CGGATCGGAA 
CTGATATTGT ACGAGAAGTC CAGCGGATTC ATAACCGAAG GCGAGTACGA GGCCATGCTG 
CAGCCTCTTA ATTCCACCAG CATCAAGTCC TTCTCACCAA AGAAATGCAC ATGGGAAACG 
ATGCCGGATA GCTACATTCC ACTCTCGTTG ACCTACGACG TCTACCAGCA GAGTCCCATG 
CTCAAGTTCC ATCTGACGCT ATCCAATGAG CAGCTGCCCG AGATGATTTC CGCTCCGGAG 
CTGCAGCGTT ATGTCCAGCA TCTGGATGCG GTGGCCGAGA TGAACTACAA CAACAATAAC 
TACAATAATA ACAACAACTG CAGCGGATTA AAGAATGGTA GTGGCGGAGG CAACAGCACG 
GTGTGCAAGA CCACACCGGA GCACATTCAG ATCGTGTACA ACTTCATGTA CAGCAATAAT 
ACCCGTCAGC AGACGGAGTA CACCCAGGAG CTGAACTGCC CGTGGTGCGG CCTGGACTGC 
CTGCGTCTCT ACGCCCTGCT GAAGCATCTA AAGCTTTGCC ACGCCCGCTT CAACTTCACC 
TACCAGCCGG CTGGAAGTGG AGCTCGCATA GATGTTACCA TCAATGATGC GTACGACGGC 
TCGTATGCCG GATCGCCCTA CGACTTGGCC GGTCCATCTG GCTCCTCGTT TGCCCGCACC 
TGCGGCCCCG TGCGACGCAC CTCGGTCACC AGTCTGATGG TGTGCCGGCC CAGGCGGCAG 
AAGACCTGCC TGGACGAATT CCTGGAGCTG GACGAGGACG AGATTAGCAA CCAGCGGTCG 
TACATTACCG GTCACAACAG ACTCTACCAT CACACGGAGA CTTGCCTACC CGTGCATCCC 
AAAGAGCTGG ACATCGACTC CGAGGGCGAG AGCGATCCGC TGTGGCTGCG CCAGAAGACC 
ATCCAGATGA TCGACGAGTT TTCCGACGTG AACGAGGGTG AAAAGGAGTT GATGAAGCTG 
TGGAACCTGC ACGTGATGCG TCACGGCTTT GTGGGCGATT GCCAGCTGCC GATCGCCTGC 
GAAATGTTCC TCGACGCCAA AGGCACTGAG ATTGTGCGCA AGAATCTCTA TCGCAACTTC 
ATCCTGCATA TGTGTTCGCT CTTCGACTAC GGACTGATTG CGGCCGAGAC GGTCTACAAG 
ACCGTACAGA AGCTTCAGGG TCTGCTCAGC AAGTACGCCG CCGGCCAGGA GCTGATGCAG 
CGGCAGCGCG AGGAGCAACT GAAGTATTGG TTGGATGTGG GCATGCACAA GAAGCAGGAA 
GACCCCAAGA CACTCAAGTC ACCCCAAAAG CCAGCACCGC CCGCAGACCA AGCATCTACC 
TCATCCGCAT CCACCTCTGG AAGTGGTTCG GGGTCCAGCT CAATGCAGCC ACCCAAACGA 
ATGCCGGCTC ATTTGAAACG AGGCAGCGCA GCGTCCTCCC CGGGCGTCCA GAGCAAGGGC 
ACTGAAAATG GCACCAACGG CAGCAACAGC AGCAGCAGCA ACAGCAAGAA CGTGGCCAAG 
AAGAGCGCCG ACCAGCCGCT TAGCACCCTG GCTAACACAA GGGAGCGACG CTCGGAGTAT 
GGCCAGAAAC GCAATGTCAG CGGATCGCGA CTGGCGGCCA CACCAGCATC GAAGCGCAAG 
CTGAGCTCCA AAGATAATAC AGTGCTTAAC AAGCGGCAGC GTTACAGCGA CGGAAGTCCC 
GGGACCGGGA TCGGAAATGG ACACGGAGGC GGAAGTGGAA GCGGAGCCAA CCGCAACAAA 
AGCAATAACC ATTCACTGCC AGCAACCAGT AACAACGCCA GTAGCAGCAG CAGCAACAGC 
AAACGAGCGA TTGCCCGGCG TCGCTCCACA TCGGAGAGGA CCAAGGCGAG CGGATCGACG 
GGAGGAGGAG CTGGTGGCGT CCGCACCAGG CTATCGGTGC CGGCCAAGTA TGAGAGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730465.1
CDS	475..3177
Translation	MAPAKKREKDSNPDGSAANGIIGLTHGAPDASNAGSTVPPTAEGQVKLNGHQQEQELFLQAFEKPTQIYRYLRNRHETNPIFLNRTLSYMKERMSRNNKKRISFQVNSMLESITQKSEAVSQNYLHVIYDSLHEKLPARLDNESGEDLLQEQLLCEAGESVSVETTLYKITRSKRKDSTLDFQELLSKCSQIVYNPKDRVGEHATISIPLQTMRPMGEQHTLYKLLFRIKVLSPSTCNDENAETPPNKRSRPNEKMFGSELILYEKSSGFITEGEYEAMLQPLNSTSIKSFSPKKCTWETMPDSYIPLSLTYDVYQQSPMLKFHLTLSNEQLPEMISAPELQRYVQHLDAVAEMNYNNNNYNNNNNCSGLKNGSGGGNSTVCKTTPEHIQIVYNFMYSNNTRQQTEYTQELNCPWCGLDCLRLYALLKHLKLCHARFNFTYQPAGSGARIDVTINDAYDGSYAGSPYDLAGPSGSSFARTCGPVRRTSVTSLMVCRPRRQKTCLDEFLELDEDEISNQRSYITGHNRLYHHTETCLPVHPKELDIDSEGESDPLWLRQKTIQMIDEFSDVNEGEKELMKLWNLHVMRHGFVGDCQLPIACEMFLDAKGTEIVRKNLYRNFILHMCSLFDYGLIAAETVYKTVQKLQGLLSKYAAGQELMQRQREEQLKYWLDVGMHKKQEDPKTLKSPQKPAPPADQASTSSASTSGSGSGSSSMQPPKRMPAHLKRGSAASSPGVQSKGTENGTNGSNSSSSNSKNVAKKSADQPLSTLANTRERRSEYGQKRNVSGSRLAATPASKRKLSSKDNTVLNKRQRYSDGSPGTGIGNGHGGGSGSGANRNKSNNHSLPATSNNASSSSSNSKRAIARRRSTSERTKASGSTGGGAGGVRTRLSVPAKYERR

Target ORF information:

RefSeq Version	NM_168826.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Su(z)12, transcript variant B (Su(z)12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168826.2

ORF Insert Sequence:

ATGGCCCCAG CGAAAAAACG CGAAAAAGAC AGTAATCCGG ATGGTTCTGC CGCAAATGGC 
ATTATTGGCC TCACCCATGG AGCACCGGAC GCGAGCAATG CTGGCTCTAC TGTACCGCCG 
ACGGCCGAGG GTCAGGTCAA ACTCAATGGC CACCAGCAGG AGCAGGAACT CTTTTTGCAG 
GCCTTTGAGA AGCCCACGCA GATCTATCGC TACCTGCGCA ATCGACACGA GACAAACCCC 
ATCTTCCTCA ACCGCACGCT CAGCTACATG AAGGAACGGA TGTCGCGGAA CAACAAGAAG 
CGGATCAGCT TCCAGGTAAA CTCCATGCTG GAGAGCATAA CACAAAAGTC GGAGGCGGTG 
AGCCAGAACT ACCTGCACGT CATCTACGAT TCGCTGCACG AGAAGTTGCC GGCGAGGTTG 
GACAACGAGT CTGGCGAGGA TTTGCTGCAG GAGCAGCTGC TCTGCGAAGC GGGCGAGTCT 
GTGAGTGTGG AGACAACGCT GTACAAGATC ACACGCAGCA AGCGAAAGGA CAGCACGCTG 
GACTTTCAGG AGCTGCTCAG CAAGTGCTCG CAGATCGTGT ACAACCCAAA GGATCGTGTG 
GGAGAACATG CCACCATCAG CATTCCTCTC CAGACGATGC GCCCAATGGG TGAGCAGCAC 
ACACTGTACA AGCTGCTATT TCGCATCAAG GTGCTGTCGC CAAGTACGTG CAATGATGAG 
AATGCAGAAA CTCCACCGAA CAAGCGGTCG CGTCCAAATG AGAAAATGTT CGGATCGGAA 
CTGATATTGT ACGAGAAGTC CAGCGGATTC ATAACCGAAG GCGAGTACGA GGCCATGCTG 
CAGCCTCTTA ATTCCACCAG CATCAAGTCC TTCTCACCAA AGAAATGCAC ATGGGAAACG 
ATGCCGGATA GCTACATTCC ACTCTCGTTG ACCTACGACG TCTACCAGCA GAGTCCCATG 
CTCAAGTTCC ATCTGACGCT ATCCAATGAG CAGCTGCCCG AGATGATTTC CGCTCCGGAG 
CTGCAGCGTT ATGTCCAGCA TCTGGATGCG GTGGCCGAGA TGAACTACAA CAACAATAAC 
TACAATAATA ACAACAACTG CAGCGGATTA AAGAATGGTA GTGGCGGAGG CAACAGCACG 
GTGTGCAAGA CCACACCGGA GCACATTCAG ATCGTGTACA ACTTCATGTA CAGCAATAAT 
ACCCGTCAGC AGACGGAGTA CACCCAGGAG CTGAACTGCC CGTGGTGCGG CCTGGACTGC 
CTGCGTCTCT ACGCCCTGCT GAAGCATCTA AAGCTTTGCC ACGCCCGCTT CAACTTCACC 
TACCAGCCGG CTGGAAGTGG AGCTCGCATA GATGTTACCA TCAATGATGC GTACGACGGC 
TCGTATGCCG GATCGCCCTA CGACTTGGCC GGTCCATCTG GCTCCTCGTT TGCCCGCACC 
TGCGGCCCCG TGCGACGCAC CTCGGTCACC AGTCTGATGG TGTGCCGGCC CAGGCGGCAG 
AAGACCTGCC TGGACGAATT CCTGGAGCTG GACGAGGACG AGATTAGCAA CCAGCGGTCG 
TACATTACCG GTCACAACAG ACTCTACCAT CACACGGAGA CTTGCCTACC CGTGCATCCC 
AAAGAGCTGG ACATCGACTC CGAGGGCGAG AGCGATCCGC TGTGGCTGCG CCAGAAGACC 
ATCCAGATGA TCGACGAGTT TTCCGACGTG AACGAGGGTG AAAAGGAGTT GATGAAGCTG 
TGGAACCTGC ACGTGATGCG TCACGGCTTT GTGGGCGATT GCCAGCTGCC GATCGCCTGC 
GAAATGTTCC TCGACGCCAA AGGCACTGAG ATTGTGCGCA AGAATCTCTA TCGCAACTTC 
ATCCTGCATA TGTGTTCGCT CTTCGACTAC GGACTGATTG CGGCCGAGAC GGTCTACAAG 
ACCGTACAGA AGCTTCAGGG TCTGCTCAGC AAGTACGCCG CCGGCCAGGA GCTGATGCAG 
CGGCAGCGCG AGGAGCAACT GAAGTATTGG TTGGATGTGG GCATGCACAA GAAGCAGGAA 
GACCCCAAGA CACTCAAGTC ACCCCAAAAG CCAGCACCGC CCGCAGACCA AGCATCTACC 
TCATCCGCAT CCACCTCTGG AAGTGGTTCG GGGTCCAGCT CAATGCAGCC ACCCAAACGA 
ATGCCGGCTC ATTTGAAACG AGGCAGCGCA GCGTCCTCCC CGGGCGTCCA GAGCAAGGGC 
ACTGAAAATG GCACCAACGG CAGCAACAGC AGCAGCAGCA ACAGCAAGAA CGTGGCCAAG 
AAGAGCGCCG ACCAGCCGCT TAGCACCCTG GCTAACACAA GGGAGCGACG CTCGGAGTAT 
GGCCAGAAAC GCAATGTCAG CGGATCGCGA CTGGCGGCCA CACCAGCATC GAAGCGCAAG 
CTGAGCTCCA AAGATAATAC AGTGCTTAAC AAGCGGCAGC GTTACAGCGA CGGAAGTCCC 
GGGACCGGGA TCGGAAATGG ACACGGAGGC GGAAGTGGAA GCGGAGCCAA CCGCAACAAA 
AGCAATAACC ATTCACTGCC AGCAACCAGT AACAACGCCA GTAGCAGCAG CAGCAACAGC 
AAACGAGCGA TTGCCCGGCG TCGCTCCACA TCGGAGAGGA CCAAGGCGAG CGGATCGACG 
GGAGGAGGAG CTGGTGGCGT CCGCACCAGG CTATCGGTGC CGGCCAAGTA TGAGAGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Su(z)12 ( NM_143802.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652059.1 Drosophila melanogaster Su(z)12, transcript variant A (Su(z)12), mRNA.

Price & Availability↑

CloneID	OFa20251
Clone ID Related Accession (Same CDS sequence)	NM_143802.3
Accession Version	NM_143802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Su(z)12, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_143802.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCCAG CGAAAAAACG CGAAAAAGAC AGTAATCCGG ATGGTTCTGC CGCAAATGGC 
ATTATTGGCC TCACCCATGG AGCACCGGAC GCGAGCAATG CTGGCTCTAC TGTACCGCCG 
ACGGCCGAGG GTCAGGTCAA ACTCAATGGC CACCAGCAGG AGCAGGAACT CTTTTTGCAG 
GCCTTTGAGA AGCCCACGCA GATCTATCGC TACCTGCGCA ATCGACACGA GACAAACCCC 
ATCTTCCTCA ACCGCACGCT CAGCTACATG AAGGAACGGA TGTCGCGGAA CAACAAGAAG 
CGGATCAGCT TCCAGGTAAA CTCCATGCTG GAGAGCATAA CACAAAAGTC GGAGGCGGTG 
AGCCAGAACT ACCTGCACGT CATCTACGAT TCGCTGCACG AGAAGTTGCC GGCGAGGTTG 
GACAACGAGT CTGGCGAGGA TTTGCTGCAG GAGCAGCTGC TCTGCGAAGC GGGCGAGTCT 
GTGAGTGTGG AGACAACGCT GTACAAGATC ACACGCAGCA AGCGAAAGGA CAGCACGCTG 
GACTTTCAGG AGCTGCTCAG CAAGTGCTCG CAGATCGTGT ACAACCCAAA GGATCGTGTG 
GGAGAACATG CCACCATCAG CATTCCTCTC CAGACGATGC GCCCAATGGG TGAGCAGCAC 
ACACTGTACA AGCTGCTATT TCGCATCAAG GTGCTGTCGC CAAGTACGTG CAATGATGAG 
AATGCAGAAA CTCCACCGAA CAAGCGGTCG CGTCCAAATG AGAAAATGTT CGGATCGGAA 
CTGATATTGT ACGAGAAGTC CAGCGGATTC ATAACCGAAG GCGAGTACGA GGCCATGCTG 
CAGCCTCTTA ATTCCACCAG CATCAAGTCC TTCTCACCAA AGAAATGCAC ATGGGAAACG 
ATGCCGGATA GCTACATTCC ACTCTCGTTG ACCTACGACG TCTACCAGCA GAGTCCCATG 
CTCAAGTTCC ATCTGACGCT ATCCAATGAG CAGCTGCCCG AGATGATTTC CGCTCCGGAG 
CTGCAGCGTT ATGTCCAGCA TCTGGATGCG GTGGCCGAGA TGAACTACAA CAACAATAAC 
TACAATAATA ACAACAACTG CAGCGGATTA AAGAATGGTA GTGGCGGAGG CAACAGCACG 
GTGTGCAAGA CCACACCGGA GCACATTCAG ATCGTGTACA ACTTCATGTA CAGCAATAAT 
ACCCGTCAGC AGACGGAGTA CACCCAGGAG CTGAACTGCC CGTGGTGCGG CCTGGACTGC 
CTGCGTCTCT ACGCCCTGCT GAAGCATCTA AAGCTTTGCC ACGCCCGCTT CAACTTCACC 
TACCAGCCGG CTGGAAGTGG AGCTCGCATA GATGTTACCA TCAATGATGC GTACGACGGC 
TCGTATGCCG GATCGCCCTA CGACTTGGCC GGTCCATCTG GCTCCTCGTT TGCCCGCACC 
TGCGGCCCCG TGCGACGCAC CTCGGTCACC AGTCTGATGG TGTGCCGGCC CAGGCGGCAG 
AAGACCTGCC TGGACGAATT CCTGGAGCTG GACGAGGACG AGATTAGCAA CCAGCGGTCG 
TACATTACCG GTCACAACAG ACTCTACCAT CACACGGAGA CTTGCCTACC CGTGCATCCC 
AAAGAGCTGG ACATCGACTC CGAGGGCGAG AGCGATCCGC TGTGGCTGCG CCAGAAGACC 
ATCCAGATGA TCGACGAGTT TTCCGACGTG AACGAGGGTG AAAAGGAGTT GATGAAGCTG 
TGGAACCTGC ACGTGATGCG TCACGGCTTT GTGGGCGATT GCCAGCTGCC GATCGCCTGC 
GAAATGTTCC TCGACGCCAA AGGCACTGAG ATTGTGCGCA AGAATCTCTA TCGCAACTTC 
ATCCTGCATA TGTGTTCGCT CTTCGACTAC GGACTGATTG CGGCCGAGAC GGTCTACAAG 
ACCGTACAGA AGCTTCAGGG TCTGCTCAGC AAGTACGCCG CCGGCCAGGA GCTGATGCAG 
CGGCAGCGCG AGGAGCAACT GAAGTATTGG TTGGATGTGG GCATGCACAA GAAGCAGGAA 
GACCCCAAGA CACTCAAGTC ACCCCAAAAG CCAGCACCGC CCGCAGACCA AGCATCTACC 
TCATCCGCAT CCACCTCTGG AAGTGGTTCG GGGTCCAGCT CAATGCAGCC ACCCAAACGA 
ATGCCGGCTC ATTTGAAACG AGGCAGCGCA GCGTCCTCCC CGGGCGTCCA GAGCAAGGGC 
ACTGAAAATG GCACCAACGG CAGCAACAGC AGCAGCAGCA ACAGCAAGAA CGTGGCCAAG 
AAGAGCGCCG ACCAGCCGCT TAGCACCCTG GCTAACACAA GGGAGCGACG CTCGGAGTAT 
GGCCAGAAAC GCAATGTCAG CGGATCGCGA CTGGCGGCCA CACCAGCATC GAAGCGCAAG 
CTGAGCTCCA AAGATGTGGA GCAGGCGGCG GATGCGCCAG AAGTGCTGAC CCACAGCGAT 
AATGCTGTCG ATGTTGGTAT TATTGATGAT GAGTGTGGCG GATTTGGTGC CGTGGGAGTT 
ATGAATGGAG TTGCGAGCCC AGTTGCCAAC AACTGTGTGG GCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652059.1
CDS	475..3042
Translation	MAPAKKREKDSNPDGSAANGIIGLTHGAPDASNAGSTVPPTAEGQVKLNGHQQEQELFLQAFEKPTQIYRYLRNRHETNPIFLNRTLSYMKERMSRNNKKRISFQVNSMLESITQKSEAVSQNYLHVIYDSLHEKLPARLDNESGEDLLQEQLLCEAGESVSVETTLYKITRSKRKDSTLDFQELLSKCSQIVYNPKDRVGEHATISIPLQTMRPMGEQHTLYKLLFRIKVLSPSTCNDENAETPPNKRSRPNEKMFGSELILYEKSSGFITEGEYEAMLQPLNSTSIKSFSPKKCTWETMPDSYIPLSLTYDVYQQSPMLKFHLTLSNEQLPEMISAPELQRYVQHLDAVAEMNYNNNNYNNNNNCSGLKNGSGGGNSTVCKTTPEHIQIVYNFMYSNNTRQQTEYTQELNCPWCGLDCLRLYALLKHLKLCHARFNFTYQPAGSGARIDVTINDAYDGSYAGSPYDLAGPSGSSFARTCGPVRRTSVTSLMVCRPRRQKTCLDEFLELDEDEISNQRSYITGHNRLYHHTETCLPVHPKELDIDSEGESDPLWLRQKTIQMIDEFSDVNEGEKELMKLWNLHVMRHGFVGDCQLPIACEMFLDAKGTEIVRKNLYRNFILHMCSLFDYGLIAAETVYKTVQKLQGLLSKYAAGQELMQRQREEQLKYWLDVGMHKKQEDPKTLKSPQKPAPPADQASTSSASTSGSGSGSSSMQPPKRMPAHLKRGSAASSPGVQSKGTENGTNGSNSSSSNSKNVAKKSADQPLSTLANTRERRSEYGQKRNVSGSRLAATPASKRKLSSKDVEQAADAPEVLTHSDNAVDVGIIDDECGGFGAVGVMNGVASPVANNCVGN

Target ORF information:

RefSeq Version	NM_143802.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Su(z)12, transcript variant A (Su(z)12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143802.3

ORF Insert Sequence:

ATGGCCCCAG CGAAAAAACG CGAAAAAGAC AGTAATCCGG ATGGTTCTGC CGCAAATGGC 
ATTATTGGCC TCACCCATGG AGCACCGGAC GCGAGCAATG CTGGCTCTAC TGTACCGCCG 
ACGGCCGAGG GTCAGGTCAA ACTCAATGGC CACCAGCAGG AGCAGGAACT CTTTTTGCAG 
GCCTTTGAGA AGCCCACGCA GATCTATCGC TACCTGCGCA ATCGACACGA GACAAACCCC 
ATCTTCCTCA ACCGCACGCT CAGCTACATG AAGGAACGGA TGTCGCGGAA CAACAAGAAG 
CGGATCAGCT TCCAGGTAAA CTCCATGCTG GAGAGCATAA CACAAAAGTC GGAGGCGGTG 
AGCCAGAACT ACCTGCACGT CATCTACGAT TCGCTGCACG AGAAGTTGCC GGCGAGGTTG 
GACAACGAGT CTGGCGAGGA TTTGCTGCAG GAGCAGCTGC TCTGCGAAGC GGGCGAGTCT 
GTGAGTGTGG AGACAACGCT GTACAAGATC ACACGCAGCA AGCGAAAGGA CAGCACGCTG 
GACTTTCAGG AGCTGCTCAG CAAGTGCTCG CAGATCGTGT ACAACCCAAA GGATCGTGTG 
GGAGAACATG CCACCATCAG CATTCCTCTC CAGACGATGC GCCCAATGGG TGAGCAGCAC 
ACACTGTACA AGCTGCTATT TCGCATCAAG GTGCTGTCGC CAAGTACGTG CAATGATGAG 
AATGCAGAAA CTCCACCGAA CAAGCGGTCG CGTCCAAATG AGAAAATGTT CGGATCGGAA 
CTGATATTGT ACGAGAAGTC CAGCGGATTC ATAACCGAAG GCGAGTACGA GGCCATGCTG 
CAGCCTCTTA ATTCCACCAG CATCAAGTCC TTCTCACCAA AGAAATGCAC ATGGGAAACG 
ATGCCGGATA GCTACATTCC ACTCTCGTTG ACCTACGACG TCTACCAGCA GAGTCCCATG 
CTCAAGTTCC ATCTGACGCT ATCCAATGAG CAGCTGCCCG AGATGATTTC CGCTCCGGAG 
CTGCAGCGTT ATGTCCAGCA TCTGGATGCG GTGGCCGAGA TGAACTACAA CAACAATAAC 
TACAATAATA ACAACAACTG CAGCGGATTA AAGAATGGTA GTGGCGGAGG CAACAGCACG 
GTGTGCAAGA CCACACCGGA GCACATTCAG ATCGTGTACA ACTTCATGTA CAGCAATAAT 
ACCCGTCAGC AGACGGAGTA CACCCAGGAG CTGAACTGCC CGTGGTGCGG CCTGGACTGC 
CTGCGTCTCT ACGCCCTGCT GAAGCATCTA AAGCTTTGCC ACGCCCGCTT CAACTTCACC 
TACCAGCCGG CTGGAAGTGG AGCTCGCATA GATGTTACCA TCAATGATGC GTACGACGGC 
TCGTATGCCG GATCGCCCTA CGACTTGGCC GGTCCATCTG GCTCCTCGTT TGCCCGCACC 
TGCGGCCCCG TGCGACGCAC CTCGGTCACC AGTCTGATGG TGTGCCGGCC CAGGCGGCAG 
AAGACCTGCC TGGACGAATT CCTGGAGCTG GACGAGGACG AGATTAGCAA CCAGCGGTCG 
TACATTACCG GTCACAACAG ACTCTACCAT CACACGGAGA CTTGCCTACC CGTGCATCCC 
AAAGAGCTGG ACATCGACTC CGAGGGCGAG AGCGATCCGC TGTGGCTGCG CCAGAAGACC 
ATCCAGATGA TCGACGAGTT TTCCGACGTG AACGAGGGTG AAAAGGAGTT GATGAAGCTG 
TGGAACCTGC ACGTGATGCG TCACGGCTTT GTGGGCGATT GCCAGCTGCC GATCGCCTGC 
GAAATGTTCC TCGACGCCAA AGGCACTGAG ATTGTGCGCA AGAATCTCTA TCGCAACTTC 
ATCCTGCATA TGTGTTCGCT CTTCGACTAC GGACTGATTG CGGCCGAGAC GGTCTACAAG 
ACCGTACAGA AGCTTCAGGG TCTGCTCAGC AAGTACGCCG CCGGCCAGGA GCTGATGCAG 
CGGCAGCGCG AGGAGCAACT GAAGTATTGG TTGGATGTGG GCATGCACAA GAAGCAGGAA 
GACCCCAAGA CACTCAAGTC ACCCCAAAAG CCAGCACCGC CCGCAGACCA AGCATCTACC 
TCATCCGCAT CCACCTCTGG AAGTGGTTCG GGGTCCAGCT CAATGCAGCC ACCCAAACGA 
ATGCCGGCTC ATTTGAAACG AGGCAGCGCA GCGTCCTCCC CGGGCGTCCA GAGCAAGGGC 
ACTGAAAATG GCACCAACGG CAGCAACAGC AGCAGCAGCA ACAGCAAGAA CGTGGCCAAG 
AAGAGCGCCG ACCAGCCGCT TAGCACCCTG GCTAACACAA GGGAGCGACG CTCGGAGTAT 
GGCCAGAAAC GCAATGTCAG CGGATCGCGA CTGGCGGCCA CACCAGCATC GAAGCGCAAG 
CTGAGCTCCA AAGATGTGGA GCAGGCGGCG GATGCGCCAG AAGTGCTGAC CCACAGCGAT 
AATGCTGTCG ATGTTGGTAT TATTGATGAT GAGTGTGGCG GATTTGGTGC CGTGGGAGTT 
ATGAATGGAG TTGCGAGCCC AGTTGCCAAC AACTGTGTGG GCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Su(z)12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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