Gpo1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpo1
Entrez Gene ID	47611
Full Name	Glycerophosphate oxidase-1
Synonyms	1(2) K05713,CG 8256,CG18786,CG8256,Dmel\CG8256,GPO,GPO-1,Gpo,alpha-GPO,alphaGPO,anon-WO0118547.114,l(2)k05713
General protein information	Preferred Names Glycerophosphate oxidase-1 Names CG8256 gene product from transcript CG8256-RC CG8256-PA CG8256-PB CG8256-PC CG8256-PD Gpo-1-PA Gpo-1-PB Gpo-1-PC Gpo-1-PD alpha glycerophosphate oxidase alpha-Glycerophosphate oxidase glycerophosphate oxidase glycerophosphate oxidase-1 lethal (2) k05713 mitochondrial GPD mitochondrial glycerol-3-phosphate dehydrogenase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52C8-52C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_137219.3	NP_611063.1	glycerophosphate oxidase 1, isoform C
NM_166119.2	NP_725496.1	glycerophosphate oxidase 1, isoform B
NM_166118.2	NP_725495.1	glycerophosphate oxidase 1, isoform A
NM_001103862.2	NP_001097332.1	glycerophosphate oxidase 1, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPD2	NP_001076581.2
Pan troglodytes (chimpanzee)	GPD2	XP_001143285.1
Xenopus tropicalis (tropical clawed frog)	gpd2	NP_001107700.1
Bos taurus (cattle)	GPD2	NP_001093766.1
Mus musculus (house mouse)	Gpd2	NP_001139292.1
Danio rerio (zebrafish)	gpd2	NP_001038813.1
Magnaporthe oryzae (rice blast fungus)	MGG_03147	XP_003716849.1
Neurospora crassa	NCU05454	XP_963445.2
	GPD2	XP_001086947.1
Canis lupus familiaris (dog)	GPD2	XP_005640453.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004437	XP_562185.3
Rattus norvegicus (Norway rat)	Gpd2	NP_036868.1
Drosophila melanogaster (fruit fly)	Gpo-1	NP_725496.1
Gallus gallus (chicken)	GPD2	XP_422168.2
Caenorhabditis elegans (roundworm)	T25G3.4	NP_492115.1
Arabidopsis thaliana (thale cress)	SDP6	NP_187648.1
Oryza sativa (rice)	Os04g0224900	NP_001052278.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Molecular analysis of alpha glycerophosphate dehydrogenase and alpha glycerophosphate oxidase mutants.
Title: The alpha glycerophosphate cycle in Drosophila melanogaster V. molecular analysis of alpha glycerophosphate dehydrogenase and alpha glycerophosphate oxidase mutants.

Gene trees for the GPDH and GPO paralogs indicate that the genes expressed in the flight muscles are evolving very slowly presumably under strong purifying selection whereas the paralogs expressed in the testes are evolving more rapidly.
Title: The alpha glycerophosphate cycle in Drosophila melanogaster VI. structure and evolution of enzyme paralogs in the genus Drosophila.

The following Gpo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11441	NM_166119.2 Latest version!	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant B (Gpo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11441	NM_137219.3 Latest version!	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant C (Gpo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11441	NM_166118.2 Latest version!	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant A (Gpo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11441	NM_001103862.2 Latest version!	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant D (Gpo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpo1 ( NM_137219.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611063.1 Drosophila melanogaster glycerophosphate oxidase 1, transcript variant C (Gpo1), mRNA.

Price & Availability↑

CloneID	OFa11441
Clone ID Related Accession (Same CDS sequence)	NM_137219.3 , NM_166119.2 , NM_166118.2 , NM_001103862.2
Accession Version	NM_137219.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	glycerophosphate oxidase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137219.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611063.1
CDS	125..2299
Translation	MTSRMFKFGVTAASACVGSVLASYTLDRWNTPHVVNNATVPPKRKRTLPPRADQIKSLMSGEEFDVLIIGGGATGAGCALDSVTRGLKTALVELDDFASGTSSRSTKLIHGGVRYLQKAILGLDLEQYRMVKEALAERATMLESAPHLTHPLPIMLPVYTWWQVPYYWVGIKAYDLVAGDRNVKSSYYLSKKDALELFPMLKKDKLCGAIVYYDGQQDDARMCLAVALTAARHGATVCNHVEVKELLKKDDGTGKQVLCGAKVKDHISGKEFTVKAKCIVNAAGPFTDSIRKMDNPTVKSICCPSSGVHIVLPGYYSPDQMGLLDPSTSDGRVIFFLPWQRQTIAGTTDLPCEITHNPTPTEDEIQFILNEIKNYLNADVEVRRGDVLSAWSGIRPLVSDPNKDDTQSLARNHIVHVSPSNLITIAGGKWTTYRAMAEHTIDAAIKACNLKPERAEAVTSYLKIEGGQGWTPTMYIRLVQDFGLECEVAQHLAKSYGDRAFAVSKMASLTGKRWPIIGNRIHPEFPYIDAEIRYGVREYACTAVDMIARRLRLAFLNVQAAQEALPVIVDIMGEELGWSKDEKERQIKHATEFLANEMGHSVNRTSKERIPIKLSKEEIQTYIKRFQLIDKDKKGYVSINDIRRALKSFGDGDVSGEQLHEILREIDTNMNGQVELDEYLQMMSAIKTGDVAYSRFARMAELEEQKHEAANLKQISVDRSGGGL

Target ORF information:

RefSeq Version	NM_137219.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant C (Gpo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137219.3

ORF Insert Sequence:

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpo1 ( NM_166119.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725496.1 Drosophila melanogaster glycerophosphate oxidase 1, transcript variant B (Gpo1), mRNA.

Price & Availability↑

CloneID	OFa11441
Clone ID Related Accession (Same CDS sequence)	NM_137219.3 , NM_166119.2 , NM_166118.2 , NM_001103862.2
Accession Version	NM_166119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	glycerophosphate oxidase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166119.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725496.1
CDS	191..2365
Translation	MTSRMFKFGVTAASACVGSVLASYTLDRWNTPHVVNNATVPPKRKRTLPPRADQIKSLMSGEEFDVLIIGGGATGAGCALDSVTRGLKTALVELDDFASGTSSRSTKLIHGGVRYLQKAILGLDLEQYRMVKEALAERATMLESAPHLTHPLPIMLPVYTWWQVPYYWVGIKAYDLVAGDRNVKSSYYLSKKDALELFPMLKKDKLCGAIVYYDGQQDDARMCLAVALTAARHGATVCNHVEVKELLKKDDGTGKQVLCGAKVKDHISGKEFTVKAKCIVNAAGPFTDSIRKMDNPTVKSICCPSSGVHIVLPGYYSPDQMGLLDPSTSDGRVIFFLPWQRQTIAGTTDLPCEITHNPTPTEDEIQFILNEIKNYLNADVEVRRGDVLSAWSGIRPLVSDPNKDDTQSLARNHIVHVSPSNLITIAGGKWTTYRAMAEHTIDAAIKACNLKPERAEAVTSYLKIEGGQGWTPTMYIRLVQDFGLECEVAQHLAKSYGDRAFAVSKMASLTGKRWPIIGNRIHPEFPYIDAEIRYGVREYACTAVDMIARRLRLAFLNVQAAQEALPVIVDIMGEELGWSKDEKERQIKHATEFLANEMGHSVNRTSKERIPIKLSKEEIQTYIKRFQLIDKDKKGYVSINDIRRALKSFGDGDVSGEQLHEILREIDTNMNGQVELDEYLQMMSAIKTGDVAYSRFARMAELEEQKHEAANLKQISVDRSGGGL

Target ORF information:

RefSeq Version	NM_166119.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant B (Gpo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166119.2

ORF Insert Sequence:

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpo1 ( NM_166118.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725495.1 Drosophila melanogaster glycerophosphate oxidase 1, transcript variant A (Gpo1), mRNA.

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CloneID	OFa11441
Clone ID Related Accession (Same CDS sequence)	NM_137219.3 , NM_166119.2 , NM_166118.2 , NM_001103862.2
Accession Version	NM_166118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	glycerophosphate oxidase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166118.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725495.1
CDS	132..2306
Translation	MTSRMFKFGVTAASACVGSVLASYTLDRWNTPHVVNNATVPPKRKRTLPPRADQIKSLMSGEEFDVLIIGGGATGAGCALDSVTRGLKTALVELDDFASGTSSRSTKLIHGGVRYLQKAILGLDLEQYRMVKEALAERATMLESAPHLTHPLPIMLPVYTWWQVPYYWVGIKAYDLVAGDRNVKSSYYLSKKDALELFPMLKKDKLCGAIVYYDGQQDDARMCLAVALTAARHGATVCNHVEVKELLKKDDGTGKQVLCGAKVKDHISGKEFTVKAKCIVNAAGPFTDSIRKMDNPTVKSICCPSSGVHIVLPGYYSPDQMGLLDPSTSDGRVIFFLPWQRQTIAGTTDLPCEITHNPTPTEDEIQFILNEIKNYLNADVEVRRGDVLSAWSGIRPLVSDPNKDDTQSLARNHIVHVSPSNLITIAGGKWTTYRAMAEHTIDAAIKACNLKPERAEAVTSYLKIEGGQGWTPTMYIRLVQDFGLECEVAQHLAKSYGDRAFAVSKMASLTGKRWPIIGNRIHPEFPYIDAEIRYGVREYACTAVDMIARRLRLAFLNVQAAQEALPVIVDIMGEELGWSKDEKERQIKHATEFLANEMGHSVNRTSKERIPIKLSKEEIQTYIKRFQLIDKDKKGYVSINDIRRALKSFGDGDVSGEQLHEILREIDTNMNGQVELDEYLQMMSAIKTGDVAYSRFARMAELEEQKHEAANLKQISVDRSGGGL

Target ORF information:

RefSeq Version	NM_166118.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant A (Gpo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166118.2

ORF Insert Sequence:

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpo1 ( NM_001103862.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097332.1 Drosophila melanogaster glycerophosphate oxidase 1, transcript variant D (Gpo1), mRNA.

Price & Availability↑

CloneID	OFa11441
Clone ID Related Accession (Same CDS sequence)	NM_137219.3 , NM_166119.2 , NM_166118.2 , NM_001103862.2
Accession Version	NM_001103862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	glycerophosphate oxidase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103862.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097332.1
CDS	336..2510
Translation	MTSRMFKFGVTAASACVGSVLASYTLDRWNTPHVVNNATVPPKRKRTLPPRADQIKSLMSGEEFDVLIIGGGATGAGCALDSVTRGLKTALVELDDFASGTSSRSTKLIHGGVRYLQKAILGLDLEQYRMVKEALAERATMLESAPHLTHPLPIMLPVYTWWQVPYYWVGIKAYDLVAGDRNVKSSYYLSKKDALELFPMLKKDKLCGAIVYYDGQQDDARMCLAVALTAARHGATVCNHVEVKELLKKDDGTGKQVLCGAKVKDHISGKEFTVKAKCIVNAAGPFTDSIRKMDNPTVKSICCPSSGVHIVLPGYYSPDQMGLLDPSTSDGRVIFFLPWQRQTIAGTTDLPCEITHNPTPTEDEIQFILNEIKNYLNADVEVRRGDVLSAWSGIRPLVSDPNKDDTQSLARNHIVHVSPSNLITIAGGKWTTYRAMAEHTIDAAIKACNLKPERAEAVTSYLKIEGGQGWTPTMYIRLVQDFGLECEVAQHLAKSYGDRAFAVSKMASLTGKRWPIIGNRIHPEFPYIDAEIRYGVREYACTAVDMIARRLRLAFLNVQAAQEALPVIVDIMGEELGWSKDEKERQIKHATEFLANEMGHSVNRTSKERIPIKLSKEEIQTYIKRFQLIDKDKKGYVSINDIRRALKSFGDGDVSGEQLHEILREIDTNMNGQVELDEYLQMMSAIKTGDVAYSRFARMAELEEQKHEAANLKQISVDRSGGGL

Target ORF information:

RefSeq Version	NM_001103862.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster glycerophosphate oxidase 1, transcript variant D (Gpo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103862.2

ORF Insert Sequence:

ATGACCTCGC GAATGTTCAA GTTCGGCGTA ACAGCCGCTA GCGCCTGTGT TGGTTCCGTC 
CTGGCCTCGT ACACCCTGGA TCGCTGGAAC ACCCCCCATG TGGTTAACAA TGCCACCGTG 
CCGCCGAAAA GGAAGCGCAC GCTGCCCCCG CGAGCGGATC AGATCAAATC CCTGATGAGC 
GGCGAGGAGT TCGACGTGCT GATCATCGGC GGAGGAGCCA CGGGAGCTGG ATGTGCTCTC 
GACTCGGTTA CAAGGGGTCT TAAGACCGCC CTGGTGGAGC TGGATGACTT TGCCAGCGGA 
ACTTCCTCGA GATCCACGAA ACTTATCCAT GGCGGAGTGC GATATCTGCA GAAGGCCATT 
TTGGGCCTAG ATCTAGAGCA GTACCGCATG GTGAAGGAGG CGCTGGCTGA GCGCGCCACC 
ATGCTCGAAT CGGCGCCCCA CTTGACCCAC CCTCTGCCCA TCATGCTGCC AGTTTACACA 
TGGTGGCAGG TTCCTTACTA CTGGGTGGGC ATCAAGGCCT ACGATCTGGT GGCCGGCGAT 
CGCAATGTGA AAAGCTCGTA CTACCTCTCC AAGAAGGATG CCCTCGAACT GTTCCCCATG 
CTCAAGAAGG ACAAGCTGTG CGGCGCTATC GTCTACTATG ATGGCCAGCA GGATGATGCC 
AGGATGTGTC TGGCGGTGGC GCTGACTGCC GCTCGTCACG GTGCCACTGT GTGCAATCAC 
GTGGAGGTGA AGGAGCTGCT GAAGAAGGAC GACGGCACTG GCAAACAGGT GCTGTGCGGA 
GCCAAGGTCA AGGACCATAT CTCCGGCAAG GAGTTCACCG TGAAGGCCAA GTGCATTGTT 
AATGCCGCCG GTCCCTTCAC GGACTCCATC CGCAAGATGG ACAACCCCAC GGTGAAGAGC 
ATCTGCTGCC CCAGTTCCGG AGTTCATATC GTACTGCCTG GCTACTATAG CCCCGATCAG 
ATGGGTCTGC TGGATCCCTC CACTTCCGAC GGCCGCGTCA TCTTCTTCCT GCCCTGGCAG 
AGGCAGACGA TTGCCGGCAC CACCGACCTG CCCTGCGAAA TCACCCACAA TCCAACGCCC 
ACCGAGGACG AGATCCAGTT TATCCTCAAC GAGATCAAGA ACTATCTGAA TGCGGATGTG 
GAGGTGCGCC GTGGCGATGT GCTCTCCGCA TGGAGCGGCA TCCGCCCTCT GGTCTCCGAT 
CCCAACAAGG ACGACACCCA GTCCCTGGCC CGCAACCACA TCGTCCACGT CAGCCCCTCC 
AATCTCATCA CCATCGCTGG CGGCAAGTGG ACCACCTACA GGGCCATGGC CGAGCACACC 
ATCGACGCGG CTATCAAGGC CTGCAACTTA AAGCCAGAGC GCGCCGAGGC AGTCACCTCC 
TATCTGAAGA TCGAAGGCGG CCAGGGCTGG ACCCCGACCA TGTACATCCG TTTGGTGCAG 
GACTTCGGAC TTGAGTGCGA GGTGGCCCAA CATCTGGCCA AGTCGTACGG TGACCGCGCC 
TTCGCCGTGT CCAAAATGGC TTCGTTGACC GGCAAGCGTT GGCCCATCAT TGGTAACCGC 
ATCCACCCCG AGTTCCCATA CATCGATGCC GAGATCCGGT ACGGAGTGCG AGAGTACGCC 
TGTACCGCTG TCGATATGAT CGCCCGTCGC CTGCGACTGG CCTTCCTCAA TGTGCAGGCA 
GCACAGGAGG CGCTGCCAGT GATCGTTGAC ATCATGGGCG AGGAGCTGGG CTGGTCCAAG 
GACGAGAAGG AGCGCCAGAT TAAGCACGCC ACTGAGTTCC TGGCCAACGA GATGGGACAC 
TCGGTCAACC GCACCTCCAA GGAGCGCATA CCCATCAAGC TGTCCAAGGA GGAGATCCAG 
ACATACATCA AGCGCTTCCA GCTGATCGAC AAAGACAAGA AGGGCTACGT TTCCATCAAC 
GATATCCGTC GCGCTCTCAA GAGCTTCGGC GATGGCGACG TCTCTGGCGA GCAGCTGCAC 
GAAATCCTGC GCGAGATCGA TACCAACATG AACGGCCAGG TGGAACTGGA CGAATACCTT 
CAGATGATGT CGGCCATCAA AACGGGCGAC GTGGCCTACT CCCGATTTGC GCGCATGGCC 
GAGCTGGAGG AGCAGAAGCA CGAGGCGGCC AATCTGAAGC AGATCAGCGT GGACCGATCC 
GGAGGCGGCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gpo1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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