Men cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Men
Entrez Gene ID	47173
Full Name	Malic enzyme
Synonyms	CG10120,Dmel\CG10120,MDH,ME,MEN,Mdh-NADP,Me,anon-WO0118547.278,mem,men
General protein information	Preferred Names Malic enzyme Names CG10120 gene product from transcript CG10120-RB CG10120-PA CG10120-PB CG10120-PC Men-PA Men-PB Men-PC NADP-dependent malate dehydrogenase NADP-malic enzyme malic enzyme
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87C6-87C7\|3-51.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275580.1	NP_001262509.1	malic enzyme, isoform C
NM_169479.2	NP_731739.1	malic enzyme, isoform A
NM_080141.4	NP_524880.2	malic enzyme, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ME1	NP_002386.1
Pan troglodytes (chimpanzee)	ME1	XP_518610.3
Bos taurus (cattle)	ME1	NP_001138325.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000184	XP_310951.5
Neurospora crassa	NCU03651	XP_960731.2
Oryza sativa (rice)	Os05g0186300	NP_001054832.1
Canis lupus familiaris (dog)	ME1	XP_532217.4
Magnaporthe oryzae (rice blast fungus)	MGG_08173	XP_003715145.1
Arabidopsis thaliana (thale cress)	NADP-ME4	NP_178093.1
Drosophila melanogaster (fruit fly)	Men	NP_731739.1
Oryza sativa (rice)	Os01g0188400	NP_001042257.1
Mus musculus (house mouse)	Me1	NP_032641.2
Rattus norvegicus (Norway rat)	Me1	NP_036732.2
Gallus gallus (chicken)	ME1	NP_989634.1
Xenopus tropicalis (tropical clawed frog)	me1	XP_002938500.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.
Title: Overexpression of malic enzyme in the larval stage extends Drosophila lifespan.

trans-interaction between alleles of the Malic enzyme (Men) locus in Drosophila melanogaster
Title: Nonclassical regulation of transcription: interchromosomal interactions at the malic enzyme locus of Drosophila melanogaster.

Studies suggest that variation in levels of cytoplasmic malic enzyme may contribute to variation in alcohol consumption.
Title: Alcohol sensitivity in Drosophila: translational potential of systems genetics.

Malic Enzyme is regulated by juvenile hormone by two mechanisms. One is by a direct effect of JH on the enzyme. The other is by stimulation of transcription of the MEN gene.
Title: Regulation of cytosolic malate dehydrogenase by juvenile hormone in Drosophila melanogaster.

The following Men gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Men cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07623	NM_001275580.1 Latest version!	Drosophila melanogaster malic enzyme, transcript variant C (Men), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06917	NM_080141.4 Latest version!	Drosophila melanogaster malic enzyme, transcript variant B (Men), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06918	NM_169479.2 Latest version!	Drosophila melanogaster malic enzyme, transcript variant A (Men), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Men ( NM_001275580.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262509.1 Drosophila melanogaster malic enzyme, transcript variant C (Men), mRNA.

Price & Availability↑

CloneID	OFa07623
Clone ID Related Accession (Same CDS sequence)	NM_001275580.1
Accession Version	NM_001275580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	malic enzyme, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAAC CCGATTCGAA ACTAGATAAA TACGCGCAGC GCGATCGCCT GGGCCTTTGG 
GGCACTGGTG ACAATGAGGT GGTCGGCAGC CTCTCCGGAT TCACCCGACT CTTGGACAAG 
CGCTACTCAA AGGGCCTGGC CTTCACACAC GAGGAGCGCC AGCAGTTGGG CATCCATGGC 
ATGCTGCCCT ATGTGGTCCG TGAGCCCAGT GAGCAGGTGG AGCACTGCCG CGCTCTGCTG 
GCGCGACTGG ATCAGGATCT GGACAAGTAC ATGTACCTGA TCAGCCTATC GGAGCGGAAC 
GAGCGTCTGT TCTACAACGT GCTCAGCTCA GACATCGCCT ACATGATGCC ACTGGTGTAC 
ACGCCCACCG TGGGATTGGC CTGCCAGCGC TACAGTTTGA TCCACCAGAA CGCCAAGGGC 
ATGTTCATAT CCATCAAGGA CAAGGGACAC ATCTACGACG TGCTAAAGAA CTGGCCGGAA 
ACGGATGTGC GTGCCATCGT TGTCACGGAC GGCGAGCGCA TCCTGGGACT GGGAGATCTG 
GGCGCCAACG GAATGGGTAT ACCCGTGGGC AAACTGTCCC TGTATACGGC CTTGGCGGGC 
ATTAAGCCAT CGCAGTGCCT GCCCATCACC TTGGATGTGG GCACCAATAC CGAATCCATC 
CTGGAGGATC CCCTGTACAT CGGTCTGCGC GAACGCAGGG CCACTGGAGA TCTGTACGAT 
GAGTTCATCG ATGAGTTCAT GCATGCCTGC GTTCGTCGCT TTGGTCAAAA CTGCCTAATC 
CAGTTCGAGG ACTTTGCCAA CGCCAATGCC TTCAGGCTGT TGTCCAAATA CCGCGACTCC 
TTCTGCACCT TCAACGACGA TATTCAAGGA ACCGCGTCGG TGGCCGTGGC TGGTCTGCTG 
GCCTCGCTAA AGATCAAGAA GACCCAGCTG AAGGATAACA CGCTGTTGTT CCTGGGCGCC 
GGAGAAGCGG CTCTTGGTAT TGCCAACCTG TGCCTGATGG CCATGAAGGT GGAGGGTCTC 
ACCGAGGAGG AGGCCAAGGC CCGCATCTGG ATGGTGGATA GCCGTGGTGT CATCACCCGC 
GATCGTCCAA AGGGCGGACT CACCGAACAC AAGCTGCACT TTGCCCAGCT GCACGAACCC 
ATCGATACTT TGGCAGAGGC GGTGCGAAAG GTGCGTCCCA ATGTCCTGAT TGGAGCGGCT 
GCGCAGGGCG GCGCCTTCAA CCAGGAGATC CTTGAGCTGA TGGCCGATAT TAATGAGACG 
CCGATCATCT TTGCACTGTC CAATCCGACC AGCAAGGCGG AGTGCACCGC CGAGGAGGCG 
TATACGTACA CCAAGGGGCG CTGCATCTTC GCCAGCGGTT CGCCTTTTGC TCCTGTGACG 
TACAACAACA AGAAGTTCTA TCCGGGTCAG GGCAACAACT CGTACATTTT CCCTGGCGTG 
GCACTGGGTG TTCTGTGTGC CGGCATGCTG AACATTCCCG AGCAGGTATT CTTGGTCGCC 
GCCGAGCGCT TGGCGGAGCT GGTCTCCAAG GACGACCTGG CCAAGGGCAG CTTGTATCCA 
CCACTCAGCT CCATTGTCAG CTGTTCGATG GCCATTGCCG AAAGGATTGT GGAGTACGCC 
TACAAAAACG GATTGGCCAC TGTTCGTCCA GAGCCGGTCA ATAAGCTGGC GTTCATCAAG 
GCCCAGATGT ACGATCTGGA CTATCCCCGA TCCGTGCCCG CCACCTATAA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262509.1
CDS	259..1995
Translation	MSKPDSKLDKYAQRDRLGLWGTGDNEVVGSLSGFTRLLDKRYSKGLAFTHEERQQLGIHGMLPYVVREPSEQVEHCRALLARLDQDLDKYMYLISLSERNERLFYNVLSSDIAYMMPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERILGLGDLGANGMGIPVGKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERRATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFCTFNDDIQGTASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWMVDSRGVITRDRPKGGLTEHKLHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQGGAFNQEILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYPGQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYPPLSSIVSCSMAIAERIVEYAYKNGLATVRPEPVNKLAFIKAQMYDLDYPRSVPATYKM

Target ORF information:

RefSeq Version	NM_001275580.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malic enzyme, transcript variant C (Men), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275580.1

ORF Insert Sequence:

ATGAGCAAAC CCGATTCGAA ACTAGATAAA TACGCGCAGC GCGATCGCCT GGGCCTTTGG 
GGCACTGGTG ACAATGAGGT GGTCGGCAGC CTCTCCGGAT TCACCCGACT CTTGGACAAG 
CGCTACTCAA AGGGCCTGGC CTTCACACAC GAGGAGCGCC AGCAGTTGGG CATCCATGGC 
ATGCTGCCCT ATGTGGTCCG TGAGCCCAGT GAGCAGGTGG AGCACTGCCG CGCTCTGCTG 
GCGCGACTGG ATCAGGATCT GGACAAGTAC ATGTACCTGA TCAGCCTATC GGAGCGGAAC 
GAGCGTCTGT TCTACAACGT GCTCAGCTCA GACATCGCCT ACATGATGCC ACTGGTGTAC 
ACGCCCACCG TGGGATTGGC CTGCCAGCGC TACAGTTTGA TCCACCAGAA CGCCAAGGGC 
ATGTTCATAT CCATCAAGGA CAAGGGACAC ATCTACGACG TGCTAAAGAA CTGGCCGGAA 
ACGGATGTGC GTGCCATCGT TGTCACGGAC GGCGAGCGCA TCCTGGGACT GGGAGATCTG 
GGCGCCAACG GAATGGGTAT ACCCGTGGGC AAACTGTCCC TGTATACGGC CTTGGCGGGC 
ATTAAGCCAT CGCAGTGCCT GCCCATCACC TTGGATGTGG GCACCAATAC CGAATCCATC 
CTGGAGGATC CCCTGTACAT CGGTCTGCGC GAACGCAGGG CCACTGGAGA TCTGTACGAT 
GAGTTCATCG ATGAGTTCAT GCATGCCTGC GTTCGTCGCT TTGGTCAAAA CTGCCTAATC 
CAGTTCGAGG ACTTTGCCAA CGCCAATGCC TTCAGGCTGT TGTCCAAATA CCGCGACTCC 
TTCTGCACCT TCAACGACGA TATTCAAGGA ACCGCGTCGG TGGCCGTGGC TGGTCTGCTG 
GCCTCGCTAA AGATCAAGAA GACCCAGCTG AAGGATAACA CGCTGTTGTT CCTGGGCGCC 
GGAGAAGCGG CTCTTGGTAT TGCCAACCTG TGCCTGATGG CCATGAAGGT GGAGGGTCTC 
ACCGAGGAGG AGGCCAAGGC CCGCATCTGG ATGGTGGATA GCCGTGGTGT CATCACCCGC 
GATCGTCCAA AGGGCGGACT CACCGAACAC AAGCTGCACT TTGCCCAGCT GCACGAACCC 
ATCGATACTT TGGCAGAGGC GGTGCGAAAG GTGCGTCCCA ATGTCCTGAT TGGAGCGGCT 
GCGCAGGGCG GCGCCTTCAA CCAGGAGATC CTTGAGCTGA TGGCCGATAT TAATGAGACG 
CCGATCATCT TTGCACTGTC CAATCCGACC AGCAAGGCGG AGTGCACCGC CGAGGAGGCG 
TATACGTACA CCAAGGGGCG CTGCATCTTC GCCAGCGGTT CGCCTTTTGC TCCTGTGACG 
TACAACAACA AGAAGTTCTA TCCGGGTCAG GGCAACAACT CGTACATTTT CCCTGGCGTG 
GCACTGGGTG TTCTGTGTGC CGGCATGCTG AACATTCCCG AGCAGGTATT CTTGGTCGCC 
GCCGAGCGCT TGGCGGAGCT GGTCTCCAAG GACGACCTGG CCAAGGGCAG CTTGTATCCA 
CCACTCAGCT CCATTGTCAG CTGTTCGATG GCCATTGCCG AAAGGATTGT GGAGTACGCC 
TACAAAAACG GATTGGCCAC TGTTCGTCCA GAGCCGGTCA ATAAGCTGGC GTTCATCAAG 
GCCCAGATGT ACGATCTGGA CTATCCCCGA TCCGTGCCCG CCACCTATAA GATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Men ( NM_080141.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524880.2 Drosophila melanogaster malic enzyme, transcript variant B (Men), mRNA.

Price & Availability↑

CloneID	OFa06917
Clone ID Related Accession (Same CDS sequence)	NM_080141.4
Accession Version	NM_080141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	malic enzyme, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_080141.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAATT CCAGTTCCAT TTGCGCCGAT CGCAATGTCA TAACAAATTT CGATGAAAAT 
GGCACGCCGG TTTATCCCAC CGCCAACAAT TCGCAGAGTC CTTCCTCATA TAGTCGCGGC 
AAAGAGCGCG AGCTCGGCTG TTACACGAAG AGAAACAGCA ACAGCAACAA CAACAATAGC 
CATGAGAGAG AGAGTCAGAG CTGTTGTAGT AGTCGTGTGT GTAAAAATCA TACGACCACA 
ACGACAACCA CACTCGAATA CGAACTTTCC AATTTCGCAA AACTAACGAC ACGAATCACA 
ACGCAAAGTG CCGCCGAAGT GGACACATCG CCGCATACGG ATACGGAAAC GCATAGGGAC 
AGAGATTCGA ATCCGGGTAA TATAGCCTTA GCCACCGATT TGGAACTGCC CAAGGGTCTG 
CCGTTATCGT TATCCTCGCG ACACCACTGG AATCAGCTGC AGAGCAGTTT GCACGCCCTT 
CACCACCAGC AACAGCAACA ACAACAGCAA CTACGTTCAT ACAGCTCCAC TAGCGAAACA 
AATTTGGAAG ACAAGATGAG CAAACCCGAT TCGAAACTAG ATAAATACGC GCAGCGCGAT 
CGCCTGGGCC TTTGGGGCAC TGGTGACAAT GAGGTGGTCG GCAGCCTCTC CGGATTCACC 
CGACTCTTGG ACAAGCGCTA CTCAAAGGGC CTGGCCTTCA CACACGAGGA GCGCCAGCAG 
TTGGGCATCC ATGGCATGCT GCCCTATGTG GTCCGTGAGC CCAGTGAGCA GGTGGAGCAC 
TGCCGCGCTC TGCTGGCGCG ACTGGATCAG GATCTGGACA AGTACATGTA CCTGATCAGC 
CTATCGGAGC GGAACGAGCG TCTGTTCTAC AACGTGCTCA GCTCAGACAT CGCCTACATG 
ATGCCACTGG TGTACACGCC CACCGTGGGA TTGGCCTGCC AGCGCTACAG TTTGATCCAC 
CAGAACGCCA AGGGCATGTT CATATCCATC AAGGACAAGG GACACATCTA CGACGTGCTA 
AAGAACTGGC CGGAAACGGA TGTGCGTGCC ATCGTTGTCA CGGACGGCGA GCGCATCCTG 
GGACTGGGAG ATCTGGGCGC CAACGGAATG GGTATACCCG TGGGCAAACT GTCCCTGTAT 
ACGGCCTTGG CGGGCATTAA GCCATCGCAG TGCCTGCCCA TCACCTTGGA TGTGGGCACC 
AATACCGAAT CCATCCTGGA GGATCCCCTG TACATCGGTC TGCGCGAACG CAGGGCCACT 
GGAGATCTGT ACGATGAGTT CATCGATGAG TTCATGCATG CCTGCGTTCG TCGCTTTGGT 
CAAAACTGCC TAATCCAGTT CGAGGACTTT GCCAACGCCA ATGCCTTCAG GCTGTTGTCC 
AAATACCGCG ACTCCTTCTG CACCTTCAAC GACGATATTC AAGGAACCGC GTCGGTGGCC 
GTGGCTGGTC TGCTGGCCTC GCTAAAGATC AAGAAGACCC AGCTGAAGGA TAACACGCTG 
TTGTTCCTGG GCGCCGGAGA AGCGGCTCTT GGTATTGCCA ACCTGTGCCT GATGGCCATG 
AAGGTGGAGG GTCTCACCGA GGAGGAGGCC AAGGCCCGCA TCTGGATGGT GGATAGCCGT 
GGTGTCATCA CCCGCGATCG TCCAAAGGGC GGACTCACCG AACACAAGCT GCACTTTGCC 
CAGCTGCACG AACCCATCGA TACTTTGGCA GAGGCGGTGC GAAAGGTGCG TCCCAATGTC 
CTGATTGGAG CGGCTGCGCA GGGCGGCGCC TTCAACCAGG AGATCCTTGA GCTGATGGCC 
GATATTAATG AGACGCCGAT CATCTTTGCA CTGTCCAATC CGACCAGCAA GGCGGAGTGC 
ACCGCCGAGG AGGCGTATAC GTACACCAAG GGGCGCTGCA TCTTCGCCAG CGGTTCGCCT 
TTTGCTCCTG TGACGTACAA CAACAAGAAG TTCTATCCGG GTCAGGGCAA CAACTCGTAC 
ATTTTCCCTG GCGTGGCACT GGGTGTTCTG TGTGCCGGCA TGCTGAACAT TCCCGAGCAG 
GTATTCTTGG TCGCCGCCGA GCGCTTGGCG GAGCTGGTCT CCAAGGACGA CCTGGCCAAG 
GGCAGCTTGT ATCCACCACT CAGCTCCATT GTCAGCTGTT CGATGGCCAT TGCCGAAAGG 
ATTGTGGAGT ACGCCTACAA AAACGGATTG GCCACTGTTC GTCCAGAGCC GGTCAATAAG 
CTGGCGTTCA TCAAGGCCCA GATGTACGAT CTGGACTATC CCCGATCCGT GCCCGCCACC 
TATAAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524880.2
CDS	246..2537
Translation	MGNSSSICADRNVITNFDENGTPVYPTANNSQSPSSYSRGKERELGCYTKRNSNSNNNNSHERESQSCCSSRVCKNHTTTTTTTLEYELSNFAKLTTRITTQSAAEVDTSPHTDTETHRDRDSNPGNIALATDLELPKGLPLSLSSRHHWNQLQSSLHALHHQQQQQQQQLRSYSSTSETNLEDKMSKPDSKLDKYAQRDRLGLWGTGDNEVVGSLSGFTRLLDKRYSKGLAFTHEERQQLGIHGMLPYVVREPSEQVEHCRALLARLDQDLDKYMYLISLSERNERLFYNVLSSDIAYMMPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERILGLGDLGANGMGIPVGKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERRATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFCTFNDDIQGTASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWMVDSRGVITRDRPKGGLTEHKLHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQGGAFNQEILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYPGQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYPPLSSIVSCSMAIAERIVEYAYKNGLATVRPEPVNKLAFIKAQMYDLDYPRSVPATYKM

Target ORF information:

RefSeq Version	NM_080141.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malic enzyme, transcript variant B (Men), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080141.4

ORF Insert Sequence:

ATGGGTAATT CCAGTTCCAT TTGCGCCGAT CGCAATGTCA TAACAAATTT CGATGAAAAT 
GGCACGCCGG TTTATCCCAC CGCCAACAAT TCGCAGAGTC CTTCCTCATA TAGTCGCGGC 
AAAGAGCGCG AGCTCGGCTG TTACACGAAG AGAAACAGCA ACAGCAACAA CAACAATAGC 
CATGAGAGAG AGAGTCAGAG CTGTTGTAGT AGTCGTGTGT GTAAAAATCA TACGACCACA 
ACGACAACCA CACTCGAATA CGAACTTTCC AATTTCGCAA AACTAACGAC ACGAATCACA 
ACGCAAAGTG CCGCCGAAGT GGACACATCG CCGCATACGG ATACGGAAAC GCATAGGGAC 
AGAGATTCGA ATCCGGGTAA TATAGCCTTA GCCACCGATT TGGAACTGCC CAAGGGTCTG 
CCGTTATCGT TATCCTCGCG ACACCACTGG AATCAGCTGC AGAGCAGTTT GCACGCCCTT 
CACCACCAGC AACAGCAACA ACAACAGCAA CTACGTTCAT ACAGCTCCAC TAGCGAAACA 
AATTTGGAAG ACAAGATGAG CAAACCCGAT TCGAAACTAG ATAAATACGC GCAGCGCGAT 
CGCCTGGGCC TTTGGGGCAC TGGTGACAAT GAGGTGGTCG GCAGCCTCTC CGGATTCACC 
CGACTCTTGG ACAAGCGCTA CTCAAAGGGC CTGGCCTTCA CACACGAGGA GCGCCAGCAG 
TTGGGCATCC ATGGCATGCT GCCCTATGTG GTCCGTGAGC CCAGTGAGCA GGTGGAGCAC 
TGCCGCGCTC TGCTGGCGCG ACTGGATCAG GATCTGGACA AGTACATGTA CCTGATCAGC 
CTATCGGAGC GGAACGAGCG TCTGTTCTAC AACGTGCTCA GCTCAGACAT CGCCTACATG 
ATGCCACTGG TGTACACGCC CACCGTGGGA TTGGCCTGCC AGCGCTACAG TTTGATCCAC 
CAGAACGCCA AGGGCATGTT CATATCCATC AAGGACAAGG GACACATCTA CGACGTGCTA 
AAGAACTGGC CGGAAACGGA TGTGCGTGCC ATCGTTGTCA CGGACGGCGA GCGCATCCTG 
GGACTGGGAG ATCTGGGCGC CAACGGAATG GGTATACCCG TGGGCAAACT GTCCCTGTAT 
ACGGCCTTGG CGGGCATTAA GCCATCGCAG TGCCTGCCCA TCACCTTGGA TGTGGGCACC 
AATACCGAAT CCATCCTGGA GGATCCCCTG TACATCGGTC TGCGCGAACG CAGGGCCACT 
GGAGATCTGT ACGATGAGTT CATCGATGAG TTCATGCATG CCTGCGTTCG TCGCTTTGGT 
CAAAACTGCC TAATCCAGTT CGAGGACTTT GCCAACGCCA ATGCCTTCAG GCTGTTGTCC 
AAATACCGCG ACTCCTTCTG CACCTTCAAC GACGATATTC AAGGAACCGC GTCGGTGGCC 
GTGGCTGGTC TGCTGGCCTC GCTAAAGATC AAGAAGACCC AGCTGAAGGA TAACACGCTG 
TTGTTCCTGG GCGCCGGAGA AGCGGCTCTT GGTATTGCCA ACCTGTGCCT GATGGCCATG 
AAGGTGGAGG GTCTCACCGA GGAGGAGGCC AAGGCCCGCA TCTGGATGGT GGATAGCCGT 
GGTGTCATCA CCCGCGATCG TCCAAAGGGC GGACTCACCG AACACAAGCT GCACTTTGCC 
CAGCTGCACG AACCCATCGA TACTTTGGCA GAGGCGGTGC GAAAGGTGCG TCCCAATGTC 
CTGATTGGAG CGGCTGCGCA GGGCGGCGCC TTCAACCAGG AGATCCTTGA GCTGATGGCC 
GATATTAATG AGACGCCGAT CATCTTTGCA CTGTCCAATC CGACCAGCAA GGCGGAGTGC 
ACCGCCGAGG AGGCGTATAC GTACACCAAG GGGCGCTGCA TCTTCGCCAG CGGTTCGCCT 
TTTGCTCCTG TGACGTACAA CAACAAGAAG TTCTATCCGG GTCAGGGCAA CAACTCGTAC 
ATTTTCCCTG GCGTGGCACT GGGTGTTCTG TGTGCCGGCA TGCTGAACAT TCCCGAGCAG 
GTATTCTTGG TCGCCGCCGA GCGCTTGGCG GAGCTGGTCT CCAAGGACGA CCTGGCCAAG 
GGCAGCTTGT ATCCACCACT CAGCTCCATT GTCAGCTGTT CGATGGCCAT TGCCGAAAGG 
ATTGTGGAGT ACGCCTACAA AAACGGATTG GCCACTGTTC GTCCAGAGCC GGTCAATAAG 
CTGGCGTTCA TCAAGGCCCA GATGTACGAT CTGGACTATC CCCGATCCGT GCCCGCCACC 
TATAAGATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Men ( NM_169479.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731739.1 Drosophila melanogaster malic enzyme, transcript variant A (Men), mRNA.

Price & Availability↑

CloneID	OFa06918
Clone ID Related Accession (Same CDS sequence)	NM_169479.2
Accession Version	NM_169479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	malic enzyme, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_169479.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACTCGA TCCTACGACG CTGTTCTGGT ATCAGAAAAA CTTTTGGACC CACGCCGGTT 
TATCCCACCG CCAACAATTC GCAGAGTCCT TCCTCATATA GTCGCGGCAA AGAGCGCGAG 
CTCGGCTGTT ACACGAAGAG AAACAGCAAC AGCAACAACA ACAATAGCCA TGAGAGAGAG 
AGTCAGAGCT GTTGTAGTAG TCGTGTGTGT AAAAATCATA CGACCACAAC GACAACCACA 
CTCGAATACG AACTTTCCAA TTTCGCAAAA CTAACGACAC GAATCACAAC GCAAAGTGCC 
GCCGAAGTGG ACACATCGCC GCATACGGAT ACGGAAACGC ATAGGGACAG AGATTCGAAT 
CCGGGTAATA TAGCCTTAGC CACCGATTTG GAACTGCCCA AGGGTCTGCC GTTATCGTTA 
TCCTCGCGAC ACCACTGGAA TCAGCTGCAG AGCAGTTTGC ACGCCCTTCA CCACCAGCAA 
CAGCAACAAC AACAGCAACT ACGTTCATAC AGCTCCACTA GCGAAACAAA TTTGGAAGAC 
AAGATGAGCA AACCCGATTC GAAACTAGAT AAATACGCGC AGCGCGATCG CCTGGGCCTT 
TGGGGCACTG GTGACAATGA GGTGGTCGGC AGCCTCTCCG GATTCACCCG ACTCTTGGAC 
AAGCGCTACT CAAAGGGCCT GGCCTTCACA CACGAGGAGC GCCAGCAGTT GGGCATCCAT 
GGCATGCTGC CCTATGTGGT CCGTGAGCCC AGTGAGCAGG TGGAGCACTG CCGCGCTCTG 
CTGGCGCGAC TGGATCAGGA TCTGGACAAG TACATGTACC TGATCAGCCT ATCGGAGCGG 
AACGAGCGTC TGTTCTACAA CGTGCTCAGC TCAGACATCG CCTACATGAT GCCACTGGTG 
TACACGCCCA CCGTGGGATT GGCCTGCCAG CGCTACAGTT TGATCCACCA GAACGCCAAG 
GGCATGTTCA TATCCATCAA GGACAAGGGA CACATCTACG ACGTGCTAAA GAACTGGCCG 
GAAACGGATG TGCGTGCCAT CGTTGTCACG GACGGCGAGC GCATCCTGGG ACTGGGAGAT 
CTGGGCGCCA ACGGAATGGG TATACCCGTG GGCAAACTGT CCCTGTATAC GGCCTTGGCG 
GGCATTAAGC CATCGCAGTG CCTGCCCATC ACCTTGGATG TGGGCACCAA TACCGAATCC 
ATCCTGGAGG ATCCCCTGTA CATCGGTCTG CGCGAACGCA GGGCCACTGG AGATCTGTAC 
GATGAGTTCA TCGATGAGTT CATGCATGCC TGCGTTCGTC GCTTTGGTCA AAACTGCCTA 
ATCCAGTTCG AGGACTTTGC CAACGCCAAT GCCTTCAGGC TGTTGTCCAA ATACCGCGAC 
TCCTTCTGCA CCTTCAACGA CGATATTCAA GGAACCGCGT CGGTGGCCGT GGCTGGTCTG 
CTGGCCTCGC TAAAGATCAA GAAGACCCAG CTGAAGGATA ACACGCTGTT GTTCCTGGGC 
GCCGGAGAAG CGGCTCTTGG TATTGCCAAC CTGTGCCTGA TGGCCATGAA GGTGGAGGGT 
CTCACCGAGG AGGAGGCCAA GGCCCGCATC TGGATGGTGG ATAGCCGTGG TGTCATCACC 
CGCGATCGTC CAAAGGGCGG ACTCACCGAA CACAAGCTGC ACTTTGCCCA GCTGCACGAA 
CCCATCGATA CTTTGGCAGA GGCGGTGCGA AAGGTGCGTC CCAATGTCCT GATTGGAGCG 
GCTGCGCAGG GCGGCGCCTT CAACCAGGAG ATCCTTGAGC TGATGGCCGA TATTAATGAG 
ACGCCGATCA TCTTTGCACT GTCCAATCCG ACCAGCAAGG CGGAGTGCAC CGCCGAGGAG 
GCGTATACGT ACACCAAGGG GCGCTGCATC TTCGCCAGCG GTTCGCCTTT TGCTCCTGTG 
ACGTACAACA ACAAGAAGTT CTATCCGGGT CAGGGCAACA ACTCGTACAT TTTCCCTGGC 
GTGGCACTGG GTGTTCTGTG TGCCGGCATG CTGAACATTC CCGAGCAGGT ATTCTTGGTC 
GCCGCCGAGC GCTTGGCGGA GCTGGTCTCC AAGGACGACC TGGCCAAGGG CAGCTTGTAT 
CCACCACTCA GCTCCATTGT CAGCTGTTCG ATGGCCATTG CCGAAAGGAT TGTGGAGTAC 
GCCTACAAAA ACGGATTGGC CACTGTTCGT CCAGAGCCGG TCAATAAGCT GGCGTTCATC 
AAGGCCCAGA TGTACGATCT GGACTATCCC CGATCCGTGC CCGCCACCTA TAAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731739.1
CDS	119..2398
Translation	MYSILRRCSGIRKTFGPTPVYPTANNSQSPSSYSRGKERELGCYTKRNSNSNNNNSHERESQSCCSSRVCKNHTTTTTTTLEYELSNFAKLTTRITTQSAAEVDTSPHTDTETHRDRDSNPGNIALATDLELPKGLPLSLSSRHHWNQLQSSLHALHHQQQQQQQQLRSYSSTSETNLEDKMSKPDSKLDKYAQRDRLGLWGTGDNEVVGSLSGFTRLLDKRYSKGLAFTHEERQQLGIHGMLPYVVREPSEQVEHCRALLARLDQDLDKYMYLISLSERNERLFYNVLSSDIAYMMPLVYTPTVGLACQRYSLIHQNAKGMFISIKDKGHIYDVLKNWPETDVRAIVVTDGERILGLGDLGANGMGIPVGKLSLYTALAGIKPSQCLPITLDVGTNTESILEDPLYIGLRERRATGDLYDEFIDEFMHACVRRFGQNCLIQFEDFANANAFRLLSKYRDSFCTFNDDIQGTASVAVAGLLASLKIKKTQLKDNTLLFLGAGEAALGIANLCLMAMKVEGLTEEEAKARIWMVDSRGVITRDRPKGGLTEHKLHFAQLHEPIDTLAEAVRKVRPNVLIGAAAQGGAFNQEILELMADINETPIIFALSNPTSKAECTAEEAYTYTKGRCIFASGSPFAPVTYNNKKFYPGQGNNSYIFPGVALGVLCAGMLNIPEQVFLVAAERLAELVSKDDLAKGSLYPPLSSIVSCSMAIAERIVEYAYKNGLATVRPEPVNKLAFIKAQMYDLDYPRSVPATYKM

Target ORF information:

RefSeq Version	NM_169479.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster malic enzyme, transcript variant A (Men), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169479.2

ORF Insert Sequence:

ATGTACTCGA TCCTACGACG CTGTTCTGGT ATCAGAAAAA CTTTTGGACC CACGCCGGTT 
TATCCCACCG CCAACAATTC GCAGAGTCCT TCCTCATATA GTCGCGGCAA AGAGCGCGAG 
CTCGGCTGTT ACACGAAGAG AAACAGCAAC AGCAACAACA ACAATAGCCA TGAGAGAGAG 
AGTCAGAGCT GTTGTAGTAG TCGTGTGTGT AAAAATCATA CGACCACAAC GACAACCACA 
CTCGAATACG AACTTTCCAA TTTCGCAAAA CTAACGACAC GAATCACAAC GCAAAGTGCC 
GCCGAAGTGG ACACATCGCC GCATACGGAT ACGGAAACGC ATAGGGACAG AGATTCGAAT 
CCGGGTAATA TAGCCTTAGC CACCGATTTG GAACTGCCCA AGGGTCTGCC GTTATCGTTA 
TCCTCGCGAC ACCACTGGAA TCAGCTGCAG AGCAGTTTGC ACGCCCTTCA CCACCAGCAA 
CAGCAACAAC AACAGCAACT ACGTTCATAC AGCTCCACTA GCGAAACAAA TTTGGAAGAC 
AAGATGAGCA AACCCGATTC GAAACTAGAT AAATACGCGC AGCGCGATCG CCTGGGCCTT 
TGGGGCACTG GTGACAATGA GGTGGTCGGC AGCCTCTCCG GATTCACCCG ACTCTTGGAC 
AAGCGCTACT CAAAGGGCCT GGCCTTCACA CACGAGGAGC GCCAGCAGTT GGGCATCCAT 
GGCATGCTGC CCTATGTGGT CCGTGAGCCC AGTGAGCAGG TGGAGCACTG CCGCGCTCTG 
CTGGCGCGAC TGGATCAGGA TCTGGACAAG TACATGTACC TGATCAGCCT ATCGGAGCGG 
AACGAGCGTC TGTTCTACAA CGTGCTCAGC TCAGACATCG CCTACATGAT GCCACTGGTG 
TACACGCCCA CCGTGGGATT GGCCTGCCAG CGCTACAGTT TGATCCACCA GAACGCCAAG 
GGCATGTTCA TATCCATCAA GGACAAGGGA CACATCTACG ACGTGCTAAA GAACTGGCCG 
GAAACGGATG TGCGTGCCAT CGTTGTCACG GACGGCGAGC GCATCCTGGG ACTGGGAGAT 
CTGGGCGCCA ACGGAATGGG TATACCCGTG GGCAAACTGT CCCTGTATAC GGCCTTGGCG 
GGCATTAAGC CATCGCAGTG CCTGCCCATC ACCTTGGATG TGGGCACCAA TACCGAATCC 
ATCCTGGAGG ATCCCCTGTA CATCGGTCTG CGCGAACGCA GGGCCACTGG AGATCTGTAC 
GATGAGTTCA TCGATGAGTT CATGCATGCC TGCGTTCGTC GCTTTGGTCA AAACTGCCTA 
ATCCAGTTCG AGGACTTTGC CAACGCCAAT GCCTTCAGGC TGTTGTCCAA ATACCGCGAC 
TCCTTCTGCA CCTTCAACGA CGATATTCAA GGAACCGCGT CGGTGGCCGT GGCTGGTCTG 
CTGGCCTCGC TAAAGATCAA GAAGACCCAG CTGAAGGATA ACACGCTGTT GTTCCTGGGC 
GCCGGAGAAG CGGCTCTTGG TATTGCCAAC CTGTGCCTGA TGGCCATGAA GGTGGAGGGT 
CTCACCGAGG AGGAGGCCAA GGCCCGCATC TGGATGGTGG ATAGCCGTGG TGTCATCACC 
CGCGATCGTC CAAAGGGCGG ACTCACCGAA CACAAGCTGC ACTTTGCCCA GCTGCACGAA 
CCCATCGATA CTTTGGCAGA GGCGGTGCGA AAGGTGCGTC CCAATGTCCT GATTGGAGCG 
GCTGCGCAGG GCGGCGCCTT CAACCAGGAG ATCCTTGAGC TGATGGCCGA TATTAATGAG 
ACGCCGATCA TCTTTGCACT GTCCAATCCG ACCAGCAAGG CGGAGTGCAC CGCCGAGGAG 
GCGTATACGT ACACCAAGGG GCGCTGCATC TTCGCCAGCG GTTCGCCTTT TGCTCCTGTG 
ACGTACAACA ACAAGAAGTT CTATCCGGGT CAGGGCAACA ACTCGTACAT TTTCCCTGGC 
GTGGCACTGG GTGTTCTGTG TGCCGGCATG CTGAACATTC CCGAGCAGGT ATTCTTGGTC 
GCCGCCGAGC GCTTGGCGGA GCTGGTCTCC AAGGACGACC TGGCCAAGGG CAGCTTGTAT 
CCACCACTCA GCTCCATTGT CAGCTGTTCG ATGGCCATTG CCGAAAGGAT TGTGGAGTAC 
GCCTACAAAA ACGGATTGGC CACTGTTCGT CCAGAGCCGG TCAATAAGCT GGCGTTCATC 
AAGGCCCAGA TGTACGATCT GGACTATCCC CGATCCGTGC CCGCCACCTA TAAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Men cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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