dup cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dup
Entrez Gene ID	47121
Full Name	double parked
Synonyms	19k,CDT1,CDT1/DUP,CG8171,Cdt1,DUP,DUP/CDT1,Dmel\CG8171,Dup,anon-WO0257455.31,cdt1,cdt1/dup,fs(2)51Fa,fs(2)PA77,fs(2)ltoPA77,l(2)51Ec,l(2)k03308
General protein information	Preferred Names double parked Names CG8171 gene product from transcript CG8171-RA CG8171-PA CG8171-PB double park double-parked doubleparked dup-PA dup-PB female sterile(2)51Fa lethal(2)51Ec speckled
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51F11-51F11

mRNA and Protein(s)

mRNA	Protein	Name
NM_080139.5	NP_524878.1	double parked, isoform A
NM_001299522.1	NP_001286451.1	double parked, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	dup	NP_524878.1
	LOC711530	XP_001100344.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005321	XP_315335.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In cycling cells, Dup destruction is coupled to DNA replication and that increased levels of Gem balance elevated Dup levels to prevent pre-replicative complex reformation when Dup degradation fails.
Title: Levels of the origin-binding protein Double parked and its inhibitor Geminin increase in response to replication stress.

phosphorylation and degradation of Dup protein at G1/S requires cyclin E/CDK2
Title: Drosophila double-parked is sufficient to induce re-replication during development and is regulated by cyclin E/CDK2.

The following dup gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dup cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12749	NM_080139.5 Latest version!	Drosophila melanogaster double parked (dup), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa12749	NM_001299522.1 Latest version!	Drosophila melanogaster double parked (dup), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dup ( NM_080139.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524878.1 Drosophila melanogaster double parked (dup), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12749
Clone ID Related Accession (Same CDS sequence)	NM_080139.5 , NM_001299522.1
Accession Version	NM_080139.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	double parked, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080139.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCAGC CATCGGTAGC TGCATTTTTC ACAAACCGCA AACGCGCCGC CTTGGATGAT 
GCTATCAGTA TCAAGAACAG GCGTTTGGTG GAACCCGCTG AAACCGTCTC TCCTGCCTCC 
GCCCCTTCCC AGTTGCCAGC CGGCGACCAG GATGCGGATC TAGACACCCT GAAGGCGGCG 
GCCACGGGCA TGCGTACCCG ATCCGGACGC ACTGCCCGAC TAATTGTCAC CGCCGCTCAA 
GAGAGCAAAA AGAAGACACC GGCTGCCGCC AAGATGGAGC CACACATCAA GCAGCCCAAG 
CTGGTGCAAT TCATTAAAAA GGGCACTCTG TCGCCCAGGA AACAGGCTCA GTCCAGTAAG 
CTGGACGAGG AGGAGCTGCA GCAGTCGTCG GCCATAAGCG AGCACACGCC CAAGGTTAAC 
TTCACCATCA CAAGCCAGCA GAATGCGGAC AATGTGCAGC GTGGCCTGCG CACACCCACC 
AAGCAGATCC TCAAGGATGC CTCGCCGATC AAGGCGGATC TCCGCCGTCA GCTCACTTTC 
GACGAGGTAA AAACGAAGGT ATCGCGGAGT GCCAAGCTGC AGGAACTCAA GGCAGTGCTG 
GCCCTTAAGG CGGCGCTCGA GCAGAAGCGC AAGGAGCAGG AGGAGCGCAA CAGGAAACTC 
CGCGACGCTG GCCCCTCCCC ATCGAAGTCC AAGATGAGCG TGCAGCTCAA GGAATTCGAC 
ACAATCGAAC TGGAGGTGCT TATAAGCCCT TTGAAGACCT TCAAGACTCC CACAAAAATA 
CCGCCACCCA CCCCGGACAA ACATGAGCTT ATGTCGCCGC GTCACACTGA CGTCTCCAAG 
CGCCTTCTCT TCAGTCCGGC CAAAAATGGA TCTCCTGTCA AATTGGTGGA GGTGCCGGCT 
TATAAGCGCT ACGCCAGTCT GGTGGAAAGC AGCCGCGCCG GCCAACTTCC ATTGCCTTAT 
AAGTACCGAC ACCTGTTGGA CGTGTTCAAG GGTCTCGATT CCGTGGTGGC CATGTTTCAC 
AACCGCAAGG AGACCATCAC ATTCAAGAAG CTTAAGCCGG CAGTGCAGCG CATGCTCCGG 
AAGAACTTCA CTGAGACCCA TTTGGCCCAG ATCAAGCACA TCTATCCAGA CGCATTTATC 
TTCAGTCAGG TGAAGACGCG CAATTTCGGT TCTGTCTCCA AAGCGGACTA TTTCCAGCTG 
ATCATCGCTC CCAATGTGGA GCCACTGCCC GAGCAACAGC AATCGGAGAA GCCACAACAT 
TTCACCAAGA TCAACGAAGA CGATGTGCTC GCGTCGGCCC AGTCGACGTC CATGAATCCA 
CACGTTATGA CGGCGCGCAT GCAGCGCTTT CAGAGCCTTC TGCTCGATCG GGCAATGCGG 
GCGCACGACC AGTTCCTTCG CTCCCAGGAT CCGCCGATCA TCATCGAAAA GGCGCTGACT 
CGCTGGCATC CGCAGTTTGA TTTGGAGAGT TGTCCGGAAG TGGAGCTGTC CCCTTTGCCG 
CAGCCGCCCA ATGTCGAGAA ATACTCGTCA GCTAAGGATA TTCTGTCTAC CGCTCGAAAT 
CTCTTCAATT GCGCCACGCC CATGGAGAGA GCAATGGATC GCTATGAGGC CAAGTTGGAG 
TCGGAAAAAC AGCAAGCAGC TGAAAGCAAT AAAAAGACGG AGGAACAGCA GGCAGGAGAA 
GTTACCAGGA CAAGTACAGC GATTCAAACC AGTCAGGAGG TGCCTGGAAT ATCTGGATCA 
TCGAAAAACC CAACTGTCCC TGAAACAACA AACCAAACAG AAACTGCCAA GCCCACCGTG 
AAGGATTGCA CAGTGCCAGA TGCCAGCTCC AATCTACTGA AGGGACTGCC AAAATCACTG 
ATCGAGAAAA TTCGAGCCAA ACAGGCAGCA AAGGCTTTGG AGGCGATGAC TCGACGTCCT 
TCGCAGGACC AGGAGGCCAC GAAGTACTCA CGTTTGCCCG AATTGGCCAG ACACCTGCGC 
AATGTCTTCG TTACCGAGCG TAAAGGAGTG CTCACCCTGG AAGTAATCAT CAAGAAGATC 
CAGAACAGCT TCCGGGCGAA CCTCACACCG CAGGAAATTG AGGCGCATCT GAAACTACTT 
GCCAAGGAGC TACCCAGCTG GGCTTCCTTT CATGAAGTGC GCAAGACCAT GTACCTCAAG 
GTTGCCAAGG ACATGGACAT GAACAAGATC ATCGAGAAGC TGGAGAGCGT GGCTAACGCC 
AAGAGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524878.1
CDS	590..2821
Translation	MAQPSVAAFFTNRKRAALDDAISIKNRRLVEPAETVSPASAPSQLPAGDQDADLDTLKAAATGMRTRSGRTARLIVTAAQESKKKTPAAAKMEPHIKQPKLVQFIKKGTLSPRKQAQSSKLDEEELQQSSAISEHTPKVNFTITSQQNADNVQRGLRTPTKQILKDASPIKADLRRQLTFDEVKTKVSRSAKLQELKAVLALKAALEQKRKEQEERNRKLRDAGPSPSKSKMSVQLKEFDTIELEVLISPLKTFKTPTKIPPPTPDKHELMSPRHTDVSKRLLFSPAKNGSPVKLVEVPAYKRYASLVESSRAGQLPLPYKYRHLLDVFKGLDSVVAMFHNRKETITFKKLKPAVQRMLRKNFTETHLAQIKHIYPDAFIFSQVKTRNFGSVSKADYFQLIIAPNVEPLPEQQQSEKPQHFTKINEDDVLASAQSTSMNPHVMTARMQRFQSLLLDRAMRAHDQFLRSQDPPIIIEKALTRWHPQFDLESCPEVELSPLPQPPNVEKYSSAKDILSTARNLFNCATPMERAMDRYEAKLESEKQQAAESNKKTEEQQAGEVTRTSTAIQTSQEVPGISGSSKNPTVPETTNQTETAKPTVKDCTVPDASSNLLKGLPKSLIEKIRAKQAAKALEAMTRRPSQDQEATKYSRLPELARHLRNVFVTERKGVLTLEVIIKKIQNSFRANLTPQEIEAHLKLLAKELPSWASFHEVRKTMYLKVAKDMDMNKIIEKLESVANAKSN

Target ORF information:

RefSeq Version	NM_080139.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster double parked (dup), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080139.5

ORF Insert Sequence:

ATGGCCCAGC CATCGGTAGC TGCATTTTTC ACAAACCGCA AACGCGCCGC CTTGGATGAT 
GCTATCAGTA TCAAGAACAG GCGTTTGGTG GAACCCGCTG AAACCGTCTC TCCTGCCTCC 
GCCCCTTCCC AGTTGCCAGC CGGCGACCAG GATGCGGATC TAGACACCCT GAAGGCGGCG 
GCCACGGGCA TGCGTACCCG ATCCGGACGC ACTGCCCGAC TAATTGTCAC CGCCGCTCAA 
GAGAGCAAAA AGAAGACACC GGCTGCCGCC AAGATGGAGC CACACATCAA GCAGCCCAAG 
CTGGTGCAAT TCATTAAAAA GGGCACTCTG TCGCCCAGGA AACAGGCTCA GTCCAGTAAG 
CTGGACGAGG AGGAGCTGCA GCAGTCGTCG GCCATAAGCG AGCACACGCC CAAGGTTAAC 
TTCACCATCA CAAGCCAGCA GAATGCGGAC AATGTGCAGC GTGGCCTGCG CACACCCACC 
AAGCAGATCC TCAAGGATGC CTCGCCGATC AAGGCGGATC TCCGCCGTCA GCTCACTTTC 
GACGAGGTAA AAACGAAGGT ATCGCGGAGT GCCAAGCTGC AGGAACTCAA GGCAGTGCTG 
GCCCTTAAGG CGGCGCTCGA GCAGAAGCGC AAGGAGCAGG AGGAGCGCAA CAGGAAACTC 
CGCGACGCTG GCCCCTCCCC ATCGAAGTCC AAGATGAGCG TGCAGCTCAA GGAATTCGAC 
ACAATCGAAC TGGAGGTGCT TATAAGCCCT TTGAAGACCT TCAAGACTCC CACAAAAATA 
CCGCCACCCA CCCCGGACAA ACATGAGCTT ATGTCGCCGC GTCACACTGA CGTCTCCAAG 
CGCCTTCTCT TCAGTCCGGC CAAAAATGGA TCTCCTGTCA AATTGGTGGA GGTGCCGGCT 
TATAAGCGCT ACGCCAGTCT GGTGGAAAGC AGCCGCGCCG GCCAACTTCC ATTGCCTTAT 
AAGTACCGAC ACCTGTTGGA CGTGTTCAAG GGTCTCGATT CCGTGGTGGC CATGTTTCAC 
AACCGCAAGG AGACCATCAC ATTCAAGAAG CTTAAGCCGG CAGTGCAGCG CATGCTCCGG 
AAGAACTTCA CTGAGACCCA TTTGGCCCAG ATCAAGCACA TCTATCCAGA CGCATTTATC 
TTCAGTCAGG TGAAGACGCG CAATTTCGGT TCTGTCTCCA AAGCGGACTA TTTCCAGCTG 
ATCATCGCTC CCAATGTGGA GCCACTGCCC GAGCAACAGC AATCGGAGAA GCCACAACAT 
TTCACCAAGA TCAACGAAGA CGATGTGCTC GCGTCGGCCC AGTCGACGTC CATGAATCCA 
CACGTTATGA CGGCGCGCAT GCAGCGCTTT CAGAGCCTTC TGCTCGATCG GGCAATGCGG 
GCGCACGACC AGTTCCTTCG CTCCCAGGAT CCGCCGATCA TCATCGAAAA GGCGCTGACT 
CGCTGGCATC CGCAGTTTGA TTTGGAGAGT TGTCCGGAAG TGGAGCTGTC CCCTTTGCCG 
CAGCCGCCCA ATGTCGAGAA ATACTCGTCA GCTAAGGATA TTCTGTCTAC CGCTCGAAAT 
CTCTTCAATT GCGCCACGCC CATGGAGAGA GCAATGGATC GCTATGAGGC CAAGTTGGAG 
TCGGAAAAAC AGCAAGCAGC TGAAAGCAAT AAAAAGACGG AGGAACAGCA GGCAGGAGAA 
GTTACCAGGA CAAGTACAGC GATTCAAACC AGTCAGGAGG TGCCTGGAAT ATCTGGATCA 
TCGAAAAACC CAACTGTCCC TGAAACAACA AACCAAACAG AAACTGCCAA GCCCACCGTG 
AAGGATTGCA CAGTGCCAGA TGCCAGCTCC AATCTACTGA AGGGACTGCC AAAATCACTG 
ATCGAGAAAA TTCGAGCCAA ACAGGCAGCA AAGGCTTTGG AGGCGATGAC TCGACGTCCT 
TCGCAGGACC AGGAGGCCAC GAAGTACTCA CGTTTGCCCG AATTGGCCAG ACACCTGCGC 
AATGTCTTCG TTACCGAGCG TAAAGGAGTG CTCACCCTGG AAGTAATCAT CAAGAAGATC 
CAGAACAGCT TCCGGGCGAA CCTCACACCG CAGGAAATTG AGGCGCATCT GAAACTACTT 
GCCAAGGAGC TACCCAGCTG GGCTTCCTTT CATGAAGTGC GCAAGACCAT GTACCTCAAG 
GTTGCCAAGG ACATGGACAT GAACAAGATC ATCGAGAAGC TGGAGAGCGT GGCTAACGCC 
AAGAGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dup ( NM_001299522.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286451.1 Drosophila melanogaster double parked (dup), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12749
Clone ID Related Accession (Same CDS sequence)	NM_080139.5 , NM_001299522.1
Accession Version	NM_001299522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	double parked, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCCAGC CATCGGTAGC TGCATTTTTC ACAAACCGCA AACGCGCCGC CTTGGATGAT 
GCTATCAGTA TCAAGAACAG GCGTTTGGTG GAACCCGCTG AAACCGTCTC TCCTGCCTCC 
GCCCCTTCCC AGTTGCCAGC CGGCGACCAG GATGCGGATC TAGACACCCT GAAGGCGGCG 
GCCACGGGCA TGCGTACCCG ATCCGGACGC ACTGCCCGAC TAATTGTCAC CGCCGCTCAA 
GAGAGCAAAA AGAAGACACC GGCTGCCGCC AAGATGGAGC CACACATCAA GCAGCCCAAG 
CTGGTGCAAT TCATTAAAAA GGGCACTCTG TCGCCCAGGA AACAGGCTCA GTCCAGTAAG 
CTGGACGAGG AGGAGCTGCA GCAGTCGTCG GCCATAAGCG AGCACACGCC CAAGGTTAAC 
TTCACCATCA CAAGCCAGCA GAATGCGGAC AATGTGCAGC GTGGCCTGCG CACACCCACC 
AAGCAGATCC TCAAGGATGC CTCGCCGATC AAGGCGGATC TCCGCCGTCA GCTCACTTTC 
GACGAGGTAA AAACGAAGGT ATCGCGGAGT GCCAAGCTGC AGGAACTCAA GGCAGTGCTG 
GCCCTTAAGG CGGCGCTCGA GCAGAAGCGC AAGGAGCAGG AGGAGCGCAA CAGGAAACTC 
CGCGACGCTG GCCCCTCCCC ATCGAAGTCC AAGATGAGCG TGCAGCTCAA GGAATTCGAC 
ACAATCGAAC TGGAGGTGCT TATAAGCCCT TTGAAGACCT TCAAGACTCC CACAAAAATA 
CCGCCACCCA CCCCGGACAA ACATGAGCTT ATGTCGCCGC GTCACACTGA CGTCTCCAAG 
CGCCTTCTCT TCAGTCCGGC CAAAAATGGA TCTCCTGTCA AATTGGTGGA GGTGCCGGCT 
TATAAGCGCT ACGCCAGTCT GGTGGAAAGC AGCCGCGCCG GCCAACTTCC ATTGCCTTAT 
AAGTACCGAC ACCTGTTGGA CGTGTTCAAG GGTCTCGATT CCGTGGTGGC CATGTTTCAC 
AACCGCAAGG AGACCATCAC ATTCAAGAAG CTTAAGCCGG CAGTGCAGCG CATGCTCCGG 
AAGAACTTCA CTGAGACCCA TTTGGCCCAG ATCAAGCACA TCTATCCAGA CGCATTTATC 
TTCAGTCAGG TGAAGACGCG CAATTTCGGT TCTGTCTCCA AAGCGGACTA TTTCCAGCTG 
ATCATCGCTC CCAATGTGGA GCCACTGCCC GAGCAACAGC AATCGGAGAA GCCACAACAT 
TTCACCAAGA TCAACGAAGA CGATGTGCTC GCGTCGGCCC AGTCGACGTC CATGAATCCA 
CACGTTATGA CGGCGCGCAT GCAGCGCTTT CAGAGCCTTC TGCTCGATCG GGCAATGCGG 
GCGCACGACC AGTTCCTTCG CTCCCAGGAT CCGCCGATCA TCATCGAAAA GGCGCTGACT 
CGCTGGCATC CGCAGTTTGA TTTGGAGAGT TGTCCGGAAG TGGAGCTGTC CCCTTTGCCG 
CAGCCGCCCA ATGTCGAGAA ATACTCGTCA GCTAAGGATA TTCTGTCTAC CGCTCGAAAT 
CTCTTCAATT GCGCCACGCC CATGGAGAGA GCAATGGATC GCTATGAGGC CAAGTTGGAG 
TCGGAAAAAC AGCAAGCAGC TGAAAGCAAT AAAAAGACGG AGGAACAGCA GGCAGGAGAA 
GTTACCAGGA CAAGTACAGC GATTCAAACC AGTCAGGAGG TGCCTGGAAT ATCTGGATCA 
TCGAAAAACC CAACTGTCCC TGAAACAACA AACCAAACAG AAACTGCCAA GCCCACCGTG 
AAGGATTGCA CAGTGCCAGA TGCCAGCTCC AATCTACTGA AGGGACTGCC AAAATCACTG 
ATCGAGAAAA TTCGAGCCAA ACAGGCAGCA AAGGCTTTGG AGGCGATGAC TCGACGTCCT 
TCGCAGGACC AGGAGGCCAC GAAGTACTCA CGTTTGCCCG AATTGGCCAG ACACCTGCGC 
AATGTCTTCG TTACCGAGCG TAAAGGAGTG CTCACCCTGG AAGTAATCAT CAAGAAGATC 
CAGAACAGCT TCCGGGCGAA CCTCACACCG CAGGAAATTG AGGCGCATCT GAAACTACTT 
GCCAAGGAGC TACCCAGCTG GGCTTCCTTT CATGAAGTGC GCAAGACCAT GTACCTCAAG 
GTTGCCAAGG ACATGGACAT GAACAAGATC ATCGAGAAGC TGGAGAGCGT GGCTAACGCC 
AAGAGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286451.1
CDS	216..2447
Translation	MAQPSVAAFFTNRKRAALDDAISIKNRRLVEPAETVSPASAPSQLPAGDQDADLDTLKAAATGMRTRSGRTARLIVTAAQESKKKTPAAAKMEPHIKQPKLVQFIKKGTLSPRKQAQSSKLDEEELQQSSAISEHTPKVNFTITSQQNADNVQRGLRTPTKQILKDASPIKADLRRQLTFDEVKTKVSRSAKLQELKAVLALKAALEQKRKEQEERNRKLRDAGPSPSKSKMSVQLKEFDTIELEVLISPLKTFKTPTKIPPPTPDKHELMSPRHTDVSKRLLFSPAKNGSPVKLVEVPAYKRYASLVESSRAGQLPLPYKYRHLLDVFKGLDSVVAMFHNRKETITFKKLKPAVQRMLRKNFTETHLAQIKHIYPDAFIFSQVKTRNFGSVSKADYFQLIIAPNVEPLPEQQQSEKPQHFTKINEDDVLASAQSTSMNPHVMTARMQRFQSLLLDRAMRAHDQFLRSQDPPIIIEKALTRWHPQFDLESCPEVELSPLPQPPNVEKYSSAKDILSTARNLFNCATPMERAMDRYEAKLESEKQQAAESNKKTEEQQAGEVTRTSTAIQTSQEVPGISGSSKNPTVPETTNQTETAKPTVKDCTVPDASSNLLKGLPKSLIEKIRAKQAAKALEAMTRRPSQDQEATKYSRLPELARHLRNVFVTERKGVLTLEVIIKKIQNSFRANLTPQEIEAHLKLLAKELPSWASFHEVRKTMYLKVAKDMDMNKIIEKLESVANAKSN

Target ORF information:

RefSeq Version	NM_001299522.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster double parked (dup), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299522.1

ORF Insert Sequence:

ATGGCCCAGC CATCGGTAGC TGCATTTTTC ACAAACCGCA AACGCGCCGC CTTGGATGAT 
GCTATCAGTA TCAAGAACAG GCGTTTGGTG GAACCCGCTG AAACCGTCTC TCCTGCCTCC 
GCCCCTTCCC AGTTGCCAGC CGGCGACCAG GATGCGGATC TAGACACCCT GAAGGCGGCG 
GCCACGGGCA TGCGTACCCG ATCCGGACGC ACTGCCCGAC TAATTGTCAC CGCCGCTCAA 
GAGAGCAAAA AGAAGACACC GGCTGCCGCC AAGATGGAGC CACACATCAA GCAGCCCAAG 
CTGGTGCAAT TCATTAAAAA GGGCACTCTG TCGCCCAGGA AACAGGCTCA GTCCAGTAAG 
CTGGACGAGG AGGAGCTGCA GCAGTCGTCG GCCATAAGCG AGCACACGCC CAAGGTTAAC 
TTCACCATCA CAAGCCAGCA GAATGCGGAC AATGTGCAGC GTGGCCTGCG CACACCCACC 
AAGCAGATCC TCAAGGATGC CTCGCCGATC AAGGCGGATC TCCGCCGTCA GCTCACTTTC 
GACGAGGTAA AAACGAAGGT ATCGCGGAGT GCCAAGCTGC AGGAACTCAA GGCAGTGCTG 
GCCCTTAAGG CGGCGCTCGA GCAGAAGCGC AAGGAGCAGG AGGAGCGCAA CAGGAAACTC 
CGCGACGCTG GCCCCTCCCC ATCGAAGTCC AAGATGAGCG TGCAGCTCAA GGAATTCGAC 
ACAATCGAAC TGGAGGTGCT TATAAGCCCT TTGAAGACCT TCAAGACTCC CACAAAAATA 
CCGCCACCCA CCCCGGACAA ACATGAGCTT ATGTCGCCGC GTCACACTGA CGTCTCCAAG 
CGCCTTCTCT TCAGTCCGGC CAAAAATGGA TCTCCTGTCA AATTGGTGGA GGTGCCGGCT 
TATAAGCGCT ACGCCAGTCT GGTGGAAAGC AGCCGCGCCG GCCAACTTCC ATTGCCTTAT 
AAGTACCGAC ACCTGTTGGA CGTGTTCAAG GGTCTCGATT CCGTGGTGGC CATGTTTCAC 
AACCGCAAGG AGACCATCAC ATTCAAGAAG CTTAAGCCGG CAGTGCAGCG CATGCTCCGG 
AAGAACTTCA CTGAGACCCA TTTGGCCCAG ATCAAGCACA TCTATCCAGA CGCATTTATC 
TTCAGTCAGG TGAAGACGCG CAATTTCGGT TCTGTCTCCA AAGCGGACTA TTTCCAGCTG 
ATCATCGCTC CCAATGTGGA GCCACTGCCC GAGCAACAGC AATCGGAGAA GCCACAACAT 
TTCACCAAGA TCAACGAAGA CGATGTGCTC GCGTCGGCCC AGTCGACGTC CATGAATCCA 
CACGTTATGA CGGCGCGCAT GCAGCGCTTT CAGAGCCTTC TGCTCGATCG GGCAATGCGG 
GCGCACGACC AGTTCCTTCG CTCCCAGGAT CCGCCGATCA TCATCGAAAA GGCGCTGACT 
CGCTGGCATC CGCAGTTTGA TTTGGAGAGT TGTCCGGAAG TGGAGCTGTC CCCTTTGCCG 
CAGCCGCCCA ATGTCGAGAA ATACTCGTCA GCTAAGGATA TTCTGTCTAC CGCTCGAAAT 
CTCTTCAATT GCGCCACGCC CATGGAGAGA GCAATGGATC GCTATGAGGC CAAGTTGGAG 
TCGGAAAAAC AGCAAGCAGC TGAAAGCAAT AAAAAGACGG AGGAACAGCA GGCAGGAGAA 
GTTACCAGGA CAAGTACAGC GATTCAAACC AGTCAGGAGG TGCCTGGAAT ATCTGGATCA 
TCGAAAAACC CAACTGTCCC TGAAACAACA AACCAAACAG AAACTGCCAA GCCCACCGTG 
AAGGATTGCA CAGTGCCAGA TGCCAGCTCC AATCTACTGA AGGGACTGCC AAAATCACTG 
ATCGAGAAAA TTCGAGCCAA ACAGGCAGCA AAGGCTTTGG AGGCGATGAC TCGACGTCCT 
TCGCAGGACC AGGAGGCCAC GAAGTACTCA CGTTTGCCCG AATTGGCCAG ACACCTGCGC 
AATGTCTTCG TTACCGAGCG TAAAGGAGTG CTCACCCTGG AAGTAATCAT CAAGAAGATC 
CAGAACAGCT TCCGGGCGAA CCTCACACCG CAGGAAATTG AGGCGCATCT GAAACTACTT 
GCCAAGGAGC TACCCAGCTG GGCTTCCTTT CATGAAGTGC GCAAGACCAT GTACCTCAAG 
GTTGCCAAGG ACATGGACAT GAACAAGATC ATCGAGAAGC TGGAGAGCGT GGCTAACGCC 
AAGAGCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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dup cDNA ORF clone, Drosophila melanogaster(fruit fly)

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