comt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	comt
Entrez Gene ID	47091
Full Name	comatose
Synonyms	CG 1618,CG1618,Comt,DmNSF,Dmel\CG1618,NSF,NSF-1,NSF1,Nsf,com,dNSF,dNSF-1,dNSF1,dNsf1,dnsf1
General protein information	Preferred Names comatose Names CG1618 gene product from transcript CG1618-RA CG1618-PA CG1618-PB N-ethyl-maleimide sensitive fusion protein N-ethylmaleimide-sensitive fusion protein NEM-sensitive fusion protein comt-PA comt-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11E8-11E8\|1-40.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080138.4	NP_524877.1	comatose, isoform A
NM_001272577.2	NP_001259506.1	comatose, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NSF	XP_511626.3
Canis lupus familiaris (dog)	NSF	XP_548044.2
Mus musculus (house mouse)	Nsf	NP_032766.2
Drosophila melanogaster (fruit fly)	comt	NP_001259506.1
Saccharomyces cerevisiae (baker's yeast)	SEC18	NP_009636.3
Gallus gallus (chicken)	NSF	NP_001019627.1
Danio rerio (zebrafish)	nsfa	NP_001037793.1
Neurospora crassa	NCU03387	XP_956834.1
Xenopus tropicalis (tropical clawed frog)	nsf	NP_001072788.1
Bos taurus (cattle)	NSF	XP_002696103.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012684	XP_307148.3
Schizosaccharomyces pombe (fission yeast)	sec18	NP_594690.1
Magnaporthe oryzae (rice blast fungus)	MGG_02418	XP_003709204.1
	NSF	XP_002800596.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076603.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076604.1
Homo sapiens (human)	NSF	NP_006169.2
Rattus norvegicus (Norway rat)	Nsf	NP_068516.1
Drosophila melanogaster (fruit fly)	Nsf2	NP_788676.1
Kluyveromyces lactis	KLLA0C14520g	XP_452850.1
Eremothecium gossypii	AGOS_AER169C	NP_985027.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study illustrated the comatose mutations in protein trafficking and synaptic vesicles function.
Title: Genetic modifiers of comatose mutations in Drosophila: insights into neuronal NSF (N-ethylmaleimide-sensitive fusion factor) functions.

Data show that NSF and SNAP exhibit activity-dependent binding to each other within living presynaptic terminals as well as distinctive interactions and mobilities.
Title: Activity-dependent interactions of NSF and SNAP at living synapses.

Results establish in vivo contributions of SNAP-25 and NSF to synaptic vesicle trafficking and define molecular mechanisms determining conserved functional properties of short-term depression.
Title: Molecular mechanisms determining conserved properties of short-term synaptic depression revealed in NSF and SNAP-25 conditional mutants.

Mutants Of NSF have been engineered that define key residues involved in two essential, but mechanistically distinct, biochemical functions of NSF: SNAP binding and SNAP-dependent ATPase stimulation.
Title: Analysis of NSF mutants reveals residues involved in SNAP binding and ATPase stimulation.

The following comt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the comt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02407	NM_080138.4 Latest version!	Drosophila melanogaster comatose (comt), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02407	NM_001272577.2 Latest version!	Drosophila melanogaster comatose (comt), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

comt ( NM_080138.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524877.1 Drosophila melanogaster comatose (comt), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02407
Clone ID Related Accession (Same CDS sequence)	NM_080138.4 , NM_001272577.2
Accession Version	NM_080138.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	comatose, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080138.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTATA TTTTGAAGGC CACCAAATGT CCCACGGACG AGCTTTCGCT GACCAATCGG 
GCTATAGTGA ATGTTGGTGA TTTTCCGGAG GAAATCAAAT ATGCGGACAT ATCGCCGGCT 
CCGGGTCAGC ACTTCATCTT TGCGCTGGAG AAAACGGTGG AGGTGCCCAG CGGCTATGTT 
GGCTTCTCCC TGGTCCAACG AAAGTGGGCC ATGGTGTCCA TTAATCAGGA ACTGGAGGTT 
CGTCCCTATC GCTTTGATGC CAGCTCGGAT GTCATCACTT GTGTGTCCTT TGAGACGGAC 
TTTCTGCAGA AGAAGACTGT CTCCCAGGAA CCCTATGACT CCGATCAAAT GGCCAAGGAG 
TTCATCATGC AATTCGCCGG AATGGCACTC ACCGTTGGCC AATCACTGGT CTTCAATTTC 
AAGGACAAGA AACTTCTCGG CCTGGCCGTC AAATCCCTAG AGGCCATCGA TCCCAAGAGC 
TTGGGCGAGG GCAAGGACAC CGCGATGCGT AATGTCCGCT TCGGCCGCAT CCTTGGCAAC 
ACCGTGGTGC AGTTCGAGAA GGCCGAGAAC AGTTCGCTGA ATCTGCAGGG CAAGAGCAAG 
GGCAAGGTGG TCCGCCAGTC CATTATAAAC CCGGATTGGG ACTTTGGCAA AATGGGCATC 
GGTGGCCTGG ACAAGGAATT CAATTCGATA TTTCGACGAG CCTTCGCATC CCGTGTCTTC 
CCACCGGAAC TGGTCGAGCA GCTGGGCTGC AAGCATGTCA AGGGCATTCT ACTTTATGGT 
CCACCGGGTA CGGGCAAAAC TCTGATGGCT CGCCAAATCG GAACTATGTT AAACGCCCGG 
GAGCCGAAAA TCGTCAACGG TCCACAGATC CTGGACAAGT ACGTGGGCGA ATCGGAGGCC 
AATGTCCGGC GTCTGTTTGC CGAGGCGGAG GAGGAGGAGA AACGCCTGGG TCCCAACAGT 
GGACTACATA TCATCATCTT CGACGAGATC GATGCCATTT GCAAGCAGCG TGGCTCCGTG 
GCCGGAAATA GTGGTGTCCA CGACACCGTC GTCAATCAGT TGCTAACCAA AATCGATGGC 
GTTGATCAGC TGAACAACAT TCTCGTTATC GGCATGACCA ATCGCAGGGA CATGATCGAT 
GAGGCTTTGC TGCGACCGGG TCGCCTCGAG GTCCAAATGG AGATCAGCCT GCCCAACGAG 
CAGGGTCGCG TCCAGATTCT CAATATCCAC ACCAAGCGGA TGCGTGAATT CAACAAGATT 
AACGACGATG TGGACAACAA GGAGATCGCT GCGCTGACCA AGAATTTCAG CGGTGCCGAG 
CTGGAGGGTT TGGTCCGTGC CGCCCAGTCG AGTGCCATGA ACCGACTGAT CAAGGCCGAT 
GCCAAGGTGA CCGTTGATCC TGAGGCCATG GAAAAGTTAA AGGTCAATCG AGATGATTTC 
CTGCACTCGC TGGAGCATGA CATTAAGCCG GCATTCGGTA CGGCCCAGGA GATTCTCGAC 
AATATGCTGG CCCGCGGTGT TATCAACTGG GGAGCACCGG TTAGCAATCT CCTCGAGGAC 
GGCATGCTGT ATGTGCAGCA GGCCAAGGCT CCAGAATCTA GCGGCTTGGT CTCCGTACTC 
GTGGCCGGAG CACCCAACTC TGGCAAGACG GCACTGGCCG CTCAGCTTGC CAAGATGTCA 
GACTTTCCGT TCGTGAAGGT CTGCTCGCCA GAGGACATGG TCGGCTACAC TGAATCGGCC 
AAGTGCCTGC ACATTCGTAA GATCTTCGAC GACGCGTATC GTTCCATGCT TAGCTGCATT 
GTGGTGGACA ATGTGGAGCG TCTGCTGGAC TACGGATCGA TTGGGCCGCG ATATTCCAAT 
ATGACGCTAC AGGCACTGCT GGTGCTGCTC AAGAAACAGC CGCCAAAGGG CCGCAAATTG 
CTCATATTGT GCACATCTAG CAGGAGGGAG GTCCTGGAAG AGATGGAGAT GCTGACGGCC 
TTCACCTCGG TGCTCCACGT GCCCAATCTT TCCAAGCCCG ACCACGTTCT GGCCGTGCTG 
GAGAACACGG ATATCTTTAG CAAGGGCGAA ATCCAGGCGA TTGGCAAGAA GATGGCCGGA 
AAACGCGTGT TTATTGGCAT CAAGAAGCTG CTGGGTCTCA TTGACATGGC TAGGCAAACG 
GAGCAATCAC AGCGGGCCAT AAAGTTCCTG TCCAAGATGG AAGAGGAGGG TGGTCTCGAC 
ATGGTGGCGC GGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524877.1
CDS	226..2463
Translation	MAYILKATKCPTDELSLTNRAIVNVGDFPEEIKYADISPAPGQHFIFALEKTVEVPSGYVGFSLVQRKWAMVSINQELEVRPYRFDASSDVITCVSFETDFLQKKTVSQEPYDSDQMAKEFIMQFAGMALTVGQSLVFNFKDKKLLGLAVKSLEAIDPKSLGEGKDTAMRNVRFGRILGNTVVQFEKAENSSLNLQGKSKGKVVRQSIINPDWDFGKMGIGGLDKEFNSIFRRAFASRVFPPELVEQLGCKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANVRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKQRGSVAGNSGVHDTVVNQLLTKIDGVDQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMREFNKINDDVDNKEIAALTKNFSGAELEGLVRAAQSSAMNRLIKADAKVTVDPEAMEKLKVNRDDFLHSLEHDIKPAFGTAQEILDNMLARGVINWGAPVSNLLEDGMLYVQQAKAPESSGLVSVLVAGAPNSGKTALAAQLAKMSDFPFVKVCSPEDMVGYTESAKCLHIRKIFDDAYRSMLSCIVVDNVERLLDYGSIGPRYSNMTLQALLVLLKKQPPKGRKLLILCTSSRREVLEEMEMLTAFTSVLHVPNLSKPDHVLAVLENTDIFSKGEIQAIGKKMAGKRVFIGIKKLLGLIDMARQTEQSQRAIKFLSKMEEEGGLDMVARQ

Target ORF information:

RefSeq Version	NM_080138.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster comatose (comt), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080138.4

ORF Insert Sequence:

ATGGCTTATA TTTTGAAGGC CACCAAATGT CCCACGGACG AGCTTTCGCT GACCAATCGG 
GCTATAGTGA ATGTTGGTGA TTTTCCGGAG GAAATCAAAT ATGCGGACAT ATCGCCGGCT 
CCGGGTCAGC ACTTCATCTT TGCGCTGGAG AAAACGGTGG AGGTGCCCAG CGGCTATGTT 
GGCTTCTCCC TGGTCCAACG AAAGTGGGCC ATGGTGTCCA TTAATCAGGA ACTGGAGGTT 
CGTCCCTATC GCTTTGATGC CAGCTCGGAT GTCATCACTT GTGTGTCCTT TGAGACGGAC 
TTTCTGCAGA AGAAGACTGT CTCCCAGGAA CCCTATGACT CCGATCAAAT GGCCAAGGAG 
TTCATCATGC AATTCGCCGG AATGGCACTC ACCGTTGGCC AATCACTGGT CTTCAATTTC 
AAGGACAAGA AACTTCTCGG CCTGGCCGTC AAATCCCTAG AGGCCATCGA TCCCAAGAGC 
TTGGGCGAGG GCAAGGACAC CGCGATGCGT AATGTCCGCT TCGGCCGCAT CCTTGGCAAC 
ACCGTGGTGC AGTTCGAGAA GGCCGAGAAC AGTTCGCTGA ATCTGCAGGG CAAGAGCAAG 
GGCAAGGTGG TCCGCCAGTC CATTATAAAC CCGGATTGGG ACTTTGGCAA AATGGGCATC 
GGTGGCCTGG ACAAGGAATT CAATTCGATA TTTCGACGAG CCTTCGCATC CCGTGTCTTC 
CCACCGGAAC TGGTCGAGCA GCTGGGCTGC AAGCATGTCA AGGGCATTCT ACTTTATGGT 
CCACCGGGTA CGGGCAAAAC TCTGATGGCT CGCCAAATCG GAACTATGTT AAACGCCCGG 
GAGCCGAAAA TCGTCAACGG TCCACAGATC CTGGACAAGT ACGTGGGCGA ATCGGAGGCC 
AATGTCCGGC GTCTGTTTGC CGAGGCGGAG GAGGAGGAGA AACGCCTGGG TCCCAACAGT 
GGACTACATA TCATCATCTT CGACGAGATC GATGCCATTT GCAAGCAGCG TGGCTCCGTG 
GCCGGAAATA GTGGTGTCCA CGACACCGTC GTCAATCAGT TGCTAACCAA AATCGATGGC 
GTTGATCAGC TGAACAACAT TCTCGTTATC GGCATGACCA ATCGCAGGGA CATGATCGAT 
GAGGCTTTGC TGCGACCGGG TCGCCTCGAG GTCCAAATGG AGATCAGCCT GCCCAACGAG 
CAGGGTCGCG TCCAGATTCT CAATATCCAC ACCAAGCGGA TGCGTGAATT CAACAAGATT 
AACGACGATG TGGACAACAA GGAGATCGCT GCGCTGACCA AGAATTTCAG CGGTGCCGAG 
CTGGAGGGTT TGGTCCGTGC CGCCCAGTCG AGTGCCATGA ACCGACTGAT CAAGGCCGAT 
GCCAAGGTGA CCGTTGATCC TGAGGCCATG GAAAAGTTAA AGGTCAATCG AGATGATTTC 
CTGCACTCGC TGGAGCATGA CATTAAGCCG GCATTCGGTA CGGCCCAGGA GATTCTCGAC 
AATATGCTGG CCCGCGGTGT TATCAACTGG GGAGCACCGG TTAGCAATCT CCTCGAGGAC 
GGCATGCTGT ATGTGCAGCA GGCCAAGGCT CCAGAATCTA GCGGCTTGGT CTCCGTACTC 
GTGGCCGGAG CACCCAACTC TGGCAAGACG GCACTGGCCG CTCAGCTTGC CAAGATGTCA 
GACTTTCCGT TCGTGAAGGT CTGCTCGCCA GAGGACATGG TCGGCTACAC TGAATCGGCC 
AAGTGCCTGC ACATTCGTAA GATCTTCGAC GACGCGTATC GTTCCATGCT TAGCTGCATT 
GTGGTGGACA ATGTGGAGCG TCTGCTGGAC TACGGATCGA TTGGGCCGCG ATATTCCAAT 
ATGACGCTAC AGGCACTGCT GGTGCTGCTC AAGAAACAGC CGCCAAAGGG CCGCAAATTG 
CTCATATTGT GCACATCTAG CAGGAGGGAG GTCCTGGAAG AGATGGAGAT GCTGACGGCC 
TTCACCTCGG TGCTCCACGT GCCCAATCTT TCCAAGCCCG ACCACGTTCT GGCCGTGCTG 
GAGAACACGG ATATCTTTAG CAAGGGCGAA ATCCAGGCGA TTGGCAAGAA GATGGCCGGA 
AAACGCGTGT TTATTGGCAT CAAGAAGCTG CTGGGTCTCA TTGACATGGC TAGGCAAACG 
GAGCAATCAC AGCGGGCCAT AAAGTTCCTG TCCAAGATGG AAGAGGAGGG TGGTCTCGAC 
ATGGTGGCGC GGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

comt ( NM_001272577.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259506.1 Drosophila melanogaster comatose (comt), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02407
Clone ID Related Accession (Same CDS sequence)	NM_080138.4 , NM_001272577.2
Accession Version	NM_001272577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	comatose, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272577.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTATA TTTTGAAGGC CACCAAATGT CCCACGGACG AGCTTTCGCT GACCAATCGG 
GCTATAGTGA ATGTTGGTGA TTTTCCGGAG GAAATCAAAT ATGCGGACAT ATCGCCGGCT 
CCGGGTCAGC ACTTCATCTT TGCGCTGGAG AAAACGGTGG AGGTGCCCAG CGGCTATGTT 
GGCTTCTCCC TGGTCCAACG AAAGTGGGCC ATGGTGTCCA TTAATCAGGA ACTGGAGGTT 
CGTCCCTATC GCTTTGATGC CAGCTCGGAT GTCATCACTT GTGTGTCCTT TGAGACGGAC 
TTTCTGCAGA AGAAGACTGT CTCCCAGGAA CCCTATGACT CCGATCAAAT GGCCAAGGAG 
TTCATCATGC AATTCGCCGG AATGGCACTC ACCGTTGGCC AATCACTGGT CTTCAATTTC 
AAGGACAAGA AACTTCTCGG CCTGGCCGTC AAATCCCTAG AGGCCATCGA TCCCAAGAGC 
TTGGGCGAGG GCAAGGACAC CGCGATGCGT AATGTCCGCT TCGGCCGCAT CCTTGGCAAC 
ACCGTGGTGC AGTTCGAGAA GGCCGAGAAC AGTTCGCTGA ATCTGCAGGG CAAGAGCAAG 
GGCAAGGTGG TCCGCCAGTC CATTATAAAC CCGGATTGGG ACTTTGGCAA AATGGGCATC 
GGTGGCCTGG ACAAGGAATT CAATTCGATA TTTCGACGAG CCTTCGCATC CCGTGTCTTC 
CCACCGGAAC TGGTCGAGCA GCTGGGCTGC AAGCATGTCA AGGGCATTCT ACTTTATGGT 
CCACCGGGTA CGGGCAAAAC TCTGATGGCT CGCCAAATCG GAACTATGTT AAACGCCCGG 
GAGCCGAAAA TCGTCAACGG TCCACAGATC CTGGACAAGT ACGTGGGCGA ATCGGAGGCC 
AATGTCCGGC GTCTGTTTGC CGAGGCGGAG GAGGAGGAGA AACGCCTGGG TCCCAACAGT 
GGACTACATA TCATCATCTT CGACGAGATC GATGCCATTT GCAAGCAGCG TGGCTCCGTG 
GCCGGAAATA GTGGTGTCCA CGACACCGTC GTCAATCAGT TGCTAACCAA AATCGATGGC 
GTTGATCAGC TGAACAACAT TCTCGTTATC GGCATGACCA ATCGCAGGGA CATGATCGAT 
GAGGCTTTGC TGCGACCGGG TCGCCTCGAG GTCCAAATGG AGATCAGCCT GCCCAACGAG 
CAGGGTCGCG TCCAGATTCT CAATATCCAC ACCAAGCGGA TGCGTGAATT CAACAAGATT 
AACGACGATG TGGACAACAA GGAGATCGCT GCGCTGACCA AGAATTTCAG CGGTGCCGAG 
CTGGAGGGTT TGGTCCGTGC CGCCCAGTCG AGTGCCATGA ACCGACTGAT CAAGGCCGAT 
GCCAAGGTGA CCGTTGATCC TGAGGCCATG GAAAAGTTAA AGGTCAATCG AGATGATTTC 
CTGCACTCGC TGGAGCATGA CATTAAGCCG GCATTCGGTA CGGCCCAGGA GATTCTCGAC 
AATATGCTGG CCCGCGGTGT TATCAACTGG GGAGCACCGG TTAGCAATCT CCTCGAGGAC 
GGCATGCTGT ATGTGCAGCA GGCCAAGGCT CCAGAATCTA GCGGCTTGGT CTCCGTACTC 
GTGGCCGGAG CACCCAACTC TGGCAAGACG GCACTGGCCG CTCAGCTTGC CAAGATGTCA 
GACTTTCCGT TCGTGAAGGT CTGCTCGCCA GAGGACATGG TCGGCTACAC TGAATCGGCC 
AAGTGCCTGC ACATTCGTAA GATCTTCGAC GACGCGTATC GTTCCATGCT TAGCTGCATT 
GTGGTGGACA ATGTGGAGCG TCTGCTGGAC TACGGATCGA TTGGGCCGCG ATATTCCAAT 
ATGACGCTAC AGGCACTGCT GGTGCTGCTC AAGAAACAGC CGCCAAAGGG CCGCAAATTG 
CTCATATTGT GCACATCTAG CAGGAGGGAG GTCCTGGAAG AGATGGAGAT GCTGACGGCC 
TTCACCTCGG TGCTCCACGT GCCCAATCTT TCCAAGCCCG ACCACGTTCT GGCCGTGCTG 
GAGAACACGG ATATCTTTAG CAAGGGCGAA ATCCAGGCGA TTGGCAAGAA GATGGCCGGA 
AAACGCGTGT TTATTGGCAT CAAGAAGCTG CTGGGTCTCA TTGACATGGC TAGGCAAACG 
GAGCAATCAC AGCGGGCCAT AAAGTTCCTG TCCAAGATGG AAGAGGAGGG TGGTCTCGAC 
ATGGTGGCGC GGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259506.1
CDS	226..2463
Translation	MAYILKATKCPTDELSLTNRAIVNVGDFPEEIKYADISPAPGQHFIFALEKTVEVPSGYVGFSLVQRKWAMVSINQELEVRPYRFDASSDVITCVSFETDFLQKKTVSQEPYDSDQMAKEFIMQFAGMALTVGQSLVFNFKDKKLLGLAVKSLEAIDPKSLGEGKDTAMRNVRFGRILGNTVVQFEKAENSSLNLQGKSKGKVVRQSIINPDWDFGKMGIGGLDKEFNSIFRRAFASRVFPPELVEQLGCKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANVRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKQRGSVAGNSGVHDTVVNQLLTKIDGVDQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMREFNKINDDVDNKEIAALTKNFSGAELEGLVRAAQSSAMNRLIKADAKVTVDPEAMEKLKVNRDDFLHSLEHDIKPAFGTAQEILDNMLARGVINWGAPVSNLLEDGMLYVQQAKAPESSGLVSVLVAGAPNSGKTALAAQLAKMSDFPFVKVCSPEDMVGYTESAKCLHIRKIFDDAYRSMLSCIVVDNVERLLDYGSIGPRYSNMTLQALLVLLKKQPPKGRKLLILCTSSRREVLEEMEMLTAFTSVLHVPNLSKPDHVLAVLENTDIFSKGEIQAIGKKMAGKRVFIGIKKLLGLIDMARQTEQSQRAIKFLSKMEEEGGLDMVARQ

Target ORF information:

RefSeq Version	NM_001272577.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster comatose (comt), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272577.2

ORF Insert Sequence:

ATGGCTTATA TTTTGAAGGC CACCAAATGT CCCACGGACG AGCTTTCGCT GACCAATCGG 
GCTATAGTGA ATGTTGGTGA TTTTCCGGAG GAAATCAAAT ATGCGGACAT ATCGCCGGCT 
CCGGGTCAGC ACTTCATCTT TGCGCTGGAG AAAACGGTGG AGGTGCCCAG CGGCTATGTT 
GGCTTCTCCC TGGTCCAACG AAAGTGGGCC ATGGTGTCCA TTAATCAGGA ACTGGAGGTT 
CGTCCCTATC GCTTTGATGC CAGCTCGGAT GTCATCACTT GTGTGTCCTT TGAGACGGAC 
TTTCTGCAGA AGAAGACTGT CTCCCAGGAA CCCTATGACT CCGATCAAAT GGCCAAGGAG 
TTCATCATGC AATTCGCCGG AATGGCACTC ACCGTTGGCC AATCACTGGT CTTCAATTTC 
AAGGACAAGA AACTTCTCGG CCTGGCCGTC AAATCCCTAG AGGCCATCGA TCCCAAGAGC 
TTGGGCGAGG GCAAGGACAC CGCGATGCGT AATGTCCGCT TCGGCCGCAT CCTTGGCAAC 
ACCGTGGTGC AGTTCGAGAA GGCCGAGAAC AGTTCGCTGA ATCTGCAGGG CAAGAGCAAG 
GGCAAGGTGG TCCGCCAGTC CATTATAAAC CCGGATTGGG ACTTTGGCAA AATGGGCATC 
GGTGGCCTGG ACAAGGAATT CAATTCGATA TTTCGACGAG CCTTCGCATC CCGTGTCTTC 
CCACCGGAAC TGGTCGAGCA GCTGGGCTGC AAGCATGTCA AGGGCATTCT ACTTTATGGT 
CCACCGGGTA CGGGCAAAAC TCTGATGGCT CGCCAAATCG GAACTATGTT AAACGCCCGG 
GAGCCGAAAA TCGTCAACGG TCCACAGATC CTGGACAAGT ACGTGGGCGA ATCGGAGGCC 
AATGTCCGGC GTCTGTTTGC CGAGGCGGAG GAGGAGGAGA AACGCCTGGG TCCCAACAGT 
GGACTACATA TCATCATCTT CGACGAGATC GATGCCATTT GCAAGCAGCG TGGCTCCGTG 
GCCGGAAATA GTGGTGTCCA CGACACCGTC GTCAATCAGT TGCTAACCAA AATCGATGGC 
GTTGATCAGC TGAACAACAT TCTCGTTATC GGCATGACCA ATCGCAGGGA CATGATCGAT 
GAGGCTTTGC TGCGACCGGG TCGCCTCGAG GTCCAAATGG AGATCAGCCT GCCCAACGAG 
CAGGGTCGCG TCCAGATTCT CAATATCCAC ACCAAGCGGA TGCGTGAATT CAACAAGATT 
AACGACGATG TGGACAACAA GGAGATCGCT GCGCTGACCA AGAATTTCAG CGGTGCCGAG 
CTGGAGGGTT TGGTCCGTGC CGCCCAGTCG AGTGCCATGA ACCGACTGAT CAAGGCCGAT 
GCCAAGGTGA CCGTTGATCC TGAGGCCATG GAAAAGTTAA AGGTCAATCG AGATGATTTC 
CTGCACTCGC TGGAGCATGA CATTAAGCCG GCATTCGGTA CGGCCCAGGA GATTCTCGAC 
AATATGCTGG CCCGCGGTGT TATCAACTGG GGAGCACCGG TTAGCAATCT CCTCGAGGAC 
GGCATGCTGT ATGTGCAGCA GGCCAAGGCT CCAGAATCTA GCGGCTTGGT CTCCGTACTC 
GTGGCCGGAG CACCCAACTC TGGCAAGACG GCACTGGCCG CTCAGCTTGC CAAGATGTCA 
GACTTTCCGT TCGTGAAGGT CTGCTCGCCA GAGGACATGG TCGGCTACAC TGAATCGGCC 
AAGTGCCTGC ACATTCGTAA GATCTTCGAC GACGCGTATC GTTCCATGCT TAGCTGCATT 
GTGGTGGACA ATGTGGAGCG TCTGCTGGAC TACGGATCGA TTGGGCCGCG ATATTCCAAT 
ATGACGCTAC AGGCACTGCT GGTGCTGCTC AAGAAACAGC CGCCAAAGGG CCGCAAATTG 
CTCATATTGT GCACATCTAG CAGGAGGGAG GTCCTGGAAG AGATGGAGAT GCTGACGGCC 
TTCACCTCGG TGCTCCACGT GCCCAATCTT TCCAAGCCCG ACCACGTTCT GGCCGTGCTG 
GAGAACACGG ATATCTTTAG CAAGGGCGAA ATCCAGGCGA TTGGCAAGAA GATGGCCGGA 
AAACGCGTGT TTATTGGCAT CAAGAAGCTG CTGGGTCTCA TTGACATGGC TAGGCAAACG 
GAGCAATCAC AGCGGGCCAT AAAGTTCCTG TCCAAGATGG AAGAGGAGGG TGGTCTCGAC 
ATGGTGGCGC GGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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comt cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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