CanB2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CanB2
Entrez Gene ID	46456
Full Name	Calcineurin B2
Synonyms	CG11217,CS2-1,Dmel\CG11217,canB2,dCNB2,dCnB2
General protein information	Preferred Names Calcineurin B2 Names CG11217 gene product from transcript CG11217-RB CG11217-PA CG11217-PB CanB2-PA CanB2-PB calcineurin B2 calcineurin suppressor, chromosome 2, group 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43E16-43E16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259263.2	NP_001246192.1	calcineurin B2, isoform B
NM_080135.4	NP_524874.2	calcineurin B2, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP3R1	XP_855383.3
Magnaporthe oryzae (rice blast fungus)	MGG_06933	XP_003709672.1
Neurospora crassa	NCU03833	XP_960592.2
Pan troglodytes (chimpanzee)	PPP3R1	XP_003309091.1
Mus musculus (house mouse)	Ppp3r1	NP_077779.2
Rattus norvegicus (Norway rat)	Ppp3r1	NP_059005.1
Eremothecium gossypii	AGOS_AER096C	NP_984956.1
Schizosaccharomyces pombe (fission yeast)	SPCC830.06	NP_588476.1
	LOC100428682	XP_002799342.1
Bos taurus (cattle)	PPP3R1	NP_777008.1
Gallus gallus (chicken)	PPP3R1	NP_989707.1
Drosophila melanogaster (fruit fly)	CanB	NP_524741.1
Arabidopsis thaliana (thale cress)	AT3G18430	NP_001189924.1
Oryza sativa (rice)	Os08g0442300	NP_001061916.2
Saccharomyces cerevisiae (baker's yeast)	CNB1	NP_012731.1
Xenopus tropicalis (tropical clawed frog)	ppp3r1	NP_989400.1
Homo sapiens (human)	PPP3R1	NP_000936.1
Danio rerio (zebrafish)	ppp3r1a	XP_692770.2
Drosophila melanogaster (fruit fly)	CanB2	NP_524874.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004298	XP_313573.5
Caenorhabditis elegans (roundworm)	cnb-1	NP_001256317.1
Kluyveromyces lactis	KLLA0D02992g	XP_453201.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate a role for Cn and calcium-dependent signal transduction in sleep and memory regulation.
Title: Pan-neuronal knockdown of calcineurin reduces sleep in the fruit fly, Drosophila melanogaster.

CanB2 has a role in flight muscle formation in Drosophila
Title: Requirement of the calcineurin subunit gene canB2 for indirect flight muscle formation in Drosophila.

The following CanB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CanB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11428	NM_080135.4 Latest version!	Drosophila melanogaster calcineurin B2, transcript variant A (CanB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11428	NM_001259263.2 Latest version!	Drosophila melanogaster calcineurin B2, transcript variant B (CanB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CanB2 ( NM_001259263.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246192.1 Drosophila melanogaster calcineurin B2, transcript variant B (CanB2), mRNA.

Price & Availability↑

CloneID	OFa11428
Clone ID Related Accession (Same CDS sequence)	NM_001259263.2 , NM_080135.4
Accession Version	NM_001259263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcineurin B2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259263.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAATG AAACATCACT GCCCATGGAA ATGTGTTCCA ATTTCGACGC CGACGAGATC 
CGGCGGCTGG GCAAACGTTT CCGGAAACTG GACCTGGACA ATTCGGGCGC GCTGAGCGTG 
GACGAGTTCA TGTCGCTGCC GGAGCTGCAA CAGAATCCGC TGGTGCAGCG CGTGATCGAC 
ATTTTCGACG CGGACGGCAA CGGCGAGGTG GACTTCAAGG AGTTCATCCA GGGCGTGTCG 
CAGTTCAGCG TCAAGGGCGA CAAGCTGTCC AAGTTGCGCT TCGCCTTTCG CATCTATGAC 
ATGGACAACG ATGGTTACAT TTCCAATGGC GAACTGTTTC AGGTTTTAAA GATGATGGTA 
GGCAATAATC TGAAGGACAC GCAACTGCAG CAGATTGTGG ACAAGACGAT CGGATTCGCG 
GATAAGGATG AGGATGGCAA GATCTCGTTC GATGAGTTCT GCTCTGTGGT CGGCAACACG 
GACATTCACA AGAAGATGGT AGTCGATGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246192.1
CDS	257..769
Translation	MGNETSLPMEMCSNFDADEIRRLGKRFRKLDLDNSGALSVDEFMSLPELQQNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIGFADKDEDGKISFDEFCSVVGNTDIHKKMVVDV

Target ORF information:

RefSeq Version	NM_001259263.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcineurin B2, transcript variant B (CanB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259263.2

ORF Insert Sequence:

ATGGGAAATG AAACATCACT GCCCATGGAA ATGTGTTCCA ATTTCGACGC CGACGAGATC 
CGGCGGCTGG GCAAACGTTT CCGGAAACTG GACCTGGACA ATTCGGGCGC GCTGAGCGTG 
GACGAGTTCA TGTCGCTGCC GGAGCTGCAA CAGAATCCGC TGGTGCAGCG CGTGATCGAC 
ATTTTCGACG CGGACGGCAA CGGCGAGGTG GACTTCAAGG AGTTCATCCA GGGCGTGTCG 
CAGTTCAGCG TCAAGGGCGA CAAGCTGTCC AAGTTGCGCT TCGCCTTTCG CATCTATGAC 
ATGGACAACG ATGGTTACAT TTCCAATGGC GAACTGTTTC AGGTTTTAAA GATGATGGTA 
GGCAATAATC TGAAGGACAC GCAACTGCAG CAGATTGTGG ACAAGACGAT CGGATTCGCG 
GATAAGGATG AGGATGGCAA GATCTCGTTC GATGAGTTCT GCTCTGTGGT CGGCAACACG 
GACATTCACA AGAAGATGGT AGTCGATGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CanB2 ( NM_080135.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524874.2 Drosophila melanogaster calcineurin B2, transcript variant A (CanB2), mRNA.

Price & Availability↑

CloneID	OFa11428
Clone ID Related Accession (Same CDS sequence)	NM_001259263.2 , NM_080135.4
Accession Version	NM_080135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	calcineurin B2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080135.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAATG AAACATCACT GCCCATGGAA ATGTGTTCCA ATTTCGACGC CGACGAGATC 
CGGCGGCTGG GCAAACGTTT CCGGAAACTG GACCTGGACA ATTCGGGCGC GCTGAGCGTG 
GACGAGTTCA TGTCGCTGCC GGAGCTGCAA CAGAATCCGC TGGTGCAGCG CGTGATCGAC 
ATTTTCGACG CGGACGGCAA CGGCGAGGTG GACTTCAAGG AGTTCATCCA GGGCGTGTCG 
CAGTTCAGCG TCAAGGGCGA CAAGCTGTCC AAGTTGCGCT TCGCCTTTCG CATCTATGAC 
ATGGACAACG ATGGTTACAT TTCCAATGGC GAACTGTTTC AGGTTTTAAA GATGATGGTA 
GGCAATAATC TGAAGGACAC GCAACTGCAG CAGATTGTGG ACAAGACGAT CGGATTCGCG 
GATAAGGATG AGGATGGCAA GATCTCGTTC GATGAGTTCT GCTCTGTGGT CGGCAACACG 
GACATTCACA AGAAGATGGT AGTCGATGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524874.2
CDS	257..769
Translation	MGNETSLPMEMCSNFDADEIRRLGKRFRKLDLDNSGALSVDEFMSLPELQQNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIGFADKDEDGKISFDEFCSVVGNTDIHKKMVVDV

Target ORF information:

RefSeq Version	NM_080135.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster calcineurin B2, transcript variant A (CanB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080135.4

ORF Insert Sequence:

ATGGGAAATG AAACATCACT GCCCATGGAA ATGTGTTCCA ATTTCGACGC CGACGAGATC 
CGGCGGCTGG GCAAACGTTT CCGGAAACTG GACCTGGACA ATTCGGGCGC GCTGAGCGTG 
GACGAGTTCA TGTCGCTGCC GGAGCTGCAA CAGAATCCGC TGGTGCAGCG CGTGATCGAC 
ATTTTCGACG CGGACGGCAA CGGCGAGGTG GACTTCAAGG AGTTCATCCA GGGCGTGTCG 
CAGTTCAGCG TCAAGGGCGA CAAGCTGTCC AAGTTGCGCT TCGCCTTTCG CATCTATGAC 
ATGGACAACG ATGGTTACAT TTCCAATGGC GAACTGTTTC AGGTTTTAAA GATGATGGTA 
GGCAATAATC TGAAGGACAC GCAACTGCAG CAGATTGTGG ACAAGACGAT CGGATTCGCG 
GATAAGGATG AGGATGGCAA GATCTCGTTC GATGAGTTCT GCTCTGTGGT CGGCAACACG 
GACATTCACA AGAAGATGGT AGTCGATGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CanB2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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