l(3)07882 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(3)07882
Entrez Gene ID	46201
Full Name	lethal (3) 07882
Synonyms	CG5824,Dmel\CG5824
General protein information	Preferred Names lethal (3) 07882 Names CG5824 gene product from transcript CG5824-RB CG5824-PA CG5824-PB l(3)07882-PA l(3)07882-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90B3-90B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316506.1	NP_001303435.1	lethal (3) 07882, isoform B
NM_143783.3	NP_652040.2	lethal (3) 07882, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	NOP14	NP_003694.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000397	XP_310703.4
Arabidopsis thaliana (thale cress)	AT1G69070	NP_177070.1
Mus musculus (house mouse)	Nop14	NP_083554.2
Rattus norvegicus (Norway rat)	Nop14	NP_001099483.1
Pan troglodytes (chimpanzee)	NOP14	XP_001151808.1
Canis lupus familiaris (dog)	NOP14	XP_536223.3
Drosophila melanogaster (fruit fly)	l(3)07882	NP_652040.2
Bos taurus (cattle)	NOP14	NP_001092466.1
Gallus gallus (chicken)	NOP14	XP_420824.2
Danio rerio (zebrafish)	nop14	NP_001003831.1
Oryza sativa (rice)	Os03g0598200	NP_001050609.1
Xenopus tropicalis (tropical clawed frog)	nop14	XP_002939267.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(3)07882 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(3)07882 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06911	NM_143783.3 Latest version!	Drosophila melanogaster lethal (3) 07882 (l(3)07882), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06911	NM_001316506.1 Latest version!	Drosophila melanogaster lethal (3) 07882 (l(3)07882), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(3)07882 ( NM_001316506.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303435.1 Drosophila melanogaster lethal (3) 07882 (l(3)07882), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa06911
Clone ID Related Accession (Same CDS sequence)	NM_001316506.1 , NM_143783.3
Accession Version	NM_001316506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) 07882, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGCCA AAGGAAAGAA GGCCAGCGCA GATGCGGTTT ACGCCAAAAA GACAACCAGG 
TCGGCCAATC CCTTCGACAA CTCGACGGCA CAGTCCAGCA AGCGGGGCAA TCCCTTCGAT 
GTGCACGTGA ACAAGGAGAA GTTCAAGATT CTGGGGAGGA TCTGCAAGCA TGACAGGGGA 
ATGCCGGGCG TGTCGCGGGC CAAGGCTCTG CAGAAACGAG CGCAGACATT GGGTCAACAG 
TTTGCCGTGA AGCACAAGAC CAATCGCTTC AAGGATAACC GAATTGGAAA ACACTTGAGT 
GGCGATCAGC TAACCGAGTC CGTGATGAAT GCCCGCTACC TGGCCGAAAA GATGTCCCAG 
GTGCGCTCCA ACCAGAAAGC GGAGAAGTTC AACCTCAACG ACGACGAACT ACTGACGCAC 
AGGGGACAAA CTCTGGAGGA GATCGAGCAG TACAGGGACG AGCGCTCCGA CGACGAGGAG 
CTAGACGACG AGGCATTAGA TGCCGATTTT ACTGCTGCTG CTCATTTTGG CGGAGAGGGA 
GACTCTGCCC AGGATAGACA GGCGGCCATC GATGAGATGA TCGTTGAACA AAAACGAAGA 
AAGAACGAAA TCGCCAAGGA GAAGGACGAA GTGTATGATC TCACAGAGAA ACTGGATGCC 
AACTATAAGC TTCTGCTGCC GCTGGTCGCC AAGGTCACGA AGGATGAGCA GGATGCCAAG 
CCACCGCCAG ATGCCTACGA TAAACTGCTC AAGGAGATGA TCTTCGAGCC GCGTGGCAGT 
GTCGCGGATA AACTAATCAA TCCCGACGAG CTGGCCAAAC AGGAGGCCGC TCGATTGGAG 
AAGCTCGAGA ACGAACGGCT TCGTCGCATG CGAGCAGATG GCGAGGAGGA TGAGGAGGCC 
TCCGTTGCCA AACCCAAGCA CCGATCCGCA GATGACCTGG ATGATGGCTA TTTCTTGGCC 
GGCGAAGATG ACGAGGGAGA TGATACGCTC GCTTACGATT TGGACGGCAA TCTGGGCACA 
CATCTCAATG GAAAGAAGGA AGCCGTGTTG AAGGGAGACG AAAATGAGGA TGACGATGAC 
AAGGAGGGGG AAGAAGAGGA AGAGGAGGAC TCGGATGAGG AGAGTGATTC AGAGGTCGAC 
AATCTGAGCG ATCTCAAGGA ATCTGAAAGT GAGTCAGAGC CAGAAGAAGC TCCGAAGGCT 
AGCAAAAAGA AAGCGAAAAA GTCTGCGGAT ACAATTCCCG CCTCCTTGGA CACCAGTATT 
CCGTTTACCA TCAAGATGCC CAAGACCTAC GAGGACTTTA CGGAGCTGCT CTCCAAGCAT 
GCTACGGCCC AGAAGGCGAT CATTATCGAA AGGATTATAA AATGTAATCA TCCCAAACTG 
GAAGGTGTTA ACCGCGAGAA CGTGGTTAAG TTGTACTCAT TTTTGCTGCA ATATCTGAAG 
GATTTGTTTG AAGATGCCAG CGAGCAGGAC ATACGAGAAC ATTTCCAGTT GCTCTCAAAG 
CTCATGCCTT ACTTATATGA ATTAACGCAA CTGAATCCCG AGCGCATGTC CAATACATTA 
CTGGAGGTGA TCAAGGAGAA GTACGAAGAG TTCAGGAAAA ATCACAAAAT GTATCCTTCA 
CTTGACACTT TGGTGTACTT CAAACTAGTG GCCAACTTGT ACTCGACTTC CGATTTCCGC 
CATCCGGTGG TCACTCCTTG CTTTATATTC ATACAACACG TCCTGTCCAG ATCACGAGTG 
CGAACGCGTC AGGAAATATC TATGGGTTTG TTCCTGGTCA CCGTAGTCTT GGAGTTTGTG 
TCCCAATCCA AGAGACTGGT TCCAGCCGTA TTCAACTTCC TGCAGGGCAT TGTACACATG 
TCCATACCGA AGAGGGATGT GGAACAGTTG GAGATCACGC CGCCTTTTGA GCGTGATGGT 
CCATTGAGCA AGCTCCTAGC ACTTCCAGCT AACACGGAAT CCACGAAGTT GGAGCCCCAA 
CAGCTGCAGC CTACTGACTT GGTCACCCAG ACGATCACTC CAGATTTCAA GGTTCGAGCA 
CTGGACACTT CCCTCCTGCT AATCAAGGAA GCTCTCCAAC TGGTGGAAGA GCATGTGGGT 
GCCTGCTACC TGGCCCAACC CTTTTTGGCA CTACTCTCCC GCCTTCCACT GGAGTCTTAC 
CCCGAGCATG TGCATCAGCA CCACAAGGAT GCCACCGAAC TGGCGGAGAA GCTAGCTGCG 
CAGAAAATGA AACCCTTGGC TCCGGCGGAA AAGAAACCCA AGGCTCTTAG GCTGCTGGAA 
CCTCGATTCG AGGCGGTTTA CGATGACAAA CGTCGTCCCA AGATGTCCAA GGCCAAGGAA 
GAGCGGGCCA AACTGCTGCA CAAGATCAAG CGCGAGAAGA AGGGTGCCAT TCGAGAAATA 
CGCAGAGATA CGTCCTTCGT TCAGATGCTT AGGCTCAAAC AGACCCTGCA AAGTGACAAG 
GAGCGCCACG AGAAGGTTAA GCGCATTTAC CAGGAGGCAT CCGTGCAACA GGGCGAGCTC 
AATGAGCTGT CCCGGGCCAA AAAGAAGAAG AAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303435.1
CDS	101..2659
Translation	MVAKGKKASADAVYAKKTTRSANPFDNSTAQSSKRGNPFDVHVNKEKFKILGRICKHDRGMPGVSRAKALQKRAQTLGQQFAVKHKTNRFKDNRIGKHLSGDQLTESVMNARYLAEKMSQVRSNQKAEKFNLNDDELLTHRGQTLEEIEQYRDERSDDEELDDEALDADFTAAAHFGGEGDSAQDRQAAIDEMIVEQKRRKNEIAKEKDEVYDLTEKLDANYKLLLPLVAKVTKDEQDAKPPPDAYDKLLKEMIFEPRGSVADKLINPDELAKQEAARLEKLENERLRRMRADGEEDEEASVAKPKHRSADDLDDGYFLAGEDDEGDDTLAYDLDGNLGTHLNGKKEAVLKGDENEDDDDKEGEEEEEEDSDEESDSEVDNLSDLKESESESEPEEAPKASKKKAKKSADTIPASLDTSIPFTIKMPKTYEDFTELLSKHATAQKAIIIERIIKCNHPKLEGVNRENVVKLYSFLLQYLKDLFEDASEQDIREHFQLLSKLMPYLYELTQLNPERMSNTLLEVIKEKYEEFRKNHKMYPSLDTLVYFKLVANLYSTSDFRHPVVTPCFIFIQHVLSRSRVRTRQEISMGLFLVTVVLEFVSQSKRLVPAVFNFLQGIVHMSIPKRDVEQLEITPPFERDGPLSKLLALPANTESTKLEPQQLQPTDLVTQTITPDFKVRALDTSLLLIKEALQLVEEHVGACYLAQPFLALLSRLPLESYPEHVHQHHKDATELAEKLAAQKMKPLAPAEKKPKALRLLEPRFEAVYDDKRRPKMSKAKEERAKLLHKIKREKKGAIREIRRDTSFVQMLRLKQTLQSDKERHEKVKRIYQEASVQQGELNELSRAKKKKKF

Target ORF information:

RefSeq Version	NM_001316506.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) 07882 (l(3)07882), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316506.1

ORF Insert Sequence:

ATGGTGGCCA AAGGAAAGAA GGCCAGCGCA GATGCGGTTT ACGCCAAAAA GACAACCAGG 
TCGGCCAATC CCTTCGACAA CTCGACGGCA CAGTCCAGCA AGCGGGGCAA TCCCTTCGAT 
GTGCACGTGA ACAAGGAGAA GTTCAAGATT CTGGGGAGGA TCTGCAAGCA TGACAGGGGA 
ATGCCGGGCG TGTCGCGGGC CAAGGCTCTG CAGAAACGAG CGCAGACATT GGGTCAACAG 
TTTGCCGTGA AGCACAAGAC CAATCGCTTC AAGGATAACC GAATTGGAAA ACACTTGAGT 
GGCGATCAGC TAACCGAGTC CGTGATGAAT GCCCGCTACC TGGCCGAAAA GATGTCCCAG 
GTGCGCTCCA ACCAGAAAGC GGAGAAGTTC AACCTCAACG ACGACGAACT ACTGACGCAC 
AGGGGACAAA CTCTGGAGGA GATCGAGCAG TACAGGGACG AGCGCTCCGA CGACGAGGAG 
CTAGACGACG AGGCATTAGA TGCCGATTTT ACTGCTGCTG CTCATTTTGG CGGAGAGGGA 
GACTCTGCCC AGGATAGACA GGCGGCCATC GATGAGATGA TCGTTGAACA AAAACGAAGA 
AAGAACGAAA TCGCCAAGGA GAAGGACGAA GTGTATGATC TCACAGAGAA ACTGGATGCC 
AACTATAAGC TTCTGCTGCC GCTGGTCGCC AAGGTCACGA AGGATGAGCA GGATGCCAAG 
CCACCGCCAG ATGCCTACGA TAAACTGCTC AAGGAGATGA TCTTCGAGCC GCGTGGCAGT 
GTCGCGGATA AACTAATCAA TCCCGACGAG CTGGCCAAAC AGGAGGCCGC TCGATTGGAG 
AAGCTCGAGA ACGAACGGCT TCGTCGCATG CGAGCAGATG GCGAGGAGGA TGAGGAGGCC 
TCCGTTGCCA AACCCAAGCA CCGATCCGCA GATGACCTGG ATGATGGCTA TTTCTTGGCC 
GGCGAAGATG ACGAGGGAGA TGATACGCTC GCTTACGATT TGGACGGCAA TCTGGGCACA 
CATCTCAATG GAAAGAAGGA AGCCGTGTTG AAGGGAGACG AAAATGAGGA TGACGATGAC 
AAGGAGGGGG AAGAAGAGGA AGAGGAGGAC TCGGATGAGG AGAGTGATTC AGAGGTCGAC 
AATCTGAGCG ATCTCAAGGA ATCTGAAAGT GAGTCAGAGC CAGAAGAAGC TCCGAAGGCT 
AGCAAAAAGA AAGCGAAAAA GTCTGCGGAT ACAATTCCCG CCTCCTTGGA CACCAGTATT 
CCGTTTACCA TCAAGATGCC CAAGACCTAC GAGGACTTTA CGGAGCTGCT CTCCAAGCAT 
GCTACGGCCC AGAAGGCGAT CATTATCGAA AGGATTATAA AATGTAATCA TCCCAAACTG 
GAAGGTGTTA ACCGCGAGAA CGTGGTTAAG TTGTACTCAT TTTTGCTGCA ATATCTGAAG 
GATTTGTTTG AAGATGCCAG CGAGCAGGAC ATACGAGAAC ATTTCCAGTT GCTCTCAAAG 
CTCATGCCTT ACTTATATGA ATTAACGCAA CTGAATCCCG AGCGCATGTC CAATACATTA 
CTGGAGGTGA TCAAGGAGAA GTACGAAGAG TTCAGGAAAA ATCACAAAAT GTATCCTTCA 
CTTGACACTT TGGTGTACTT CAAACTAGTG GCCAACTTGT ACTCGACTTC CGATTTCCGC 
CATCCGGTGG TCACTCCTTG CTTTATATTC ATACAACACG TCCTGTCCAG ATCACGAGTG 
CGAACGCGTC AGGAAATATC TATGGGTTTG TTCCTGGTCA CCGTAGTCTT GGAGTTTGTG 
TCCCAATCCA AGAGACTGGT TCCAGCCGTA TTCAACTTCC TGCAGGGCAT TGTACACATG 
TCCATACCGA AGAGGGATGT GGAACAGTTG GAGATCACGC CGCCTTTTGA GCGTGATGGT 
CCATTGAGCA AGCTCCTAGC ACTTCCAGCT AACACGGAAT CCACGAAGTT GGAGCCCCAA 
CAGCTGCAGC CTACTGACTT GGTCACCCAG ACGATCACTC CAGATTTCAA GGTTCGAGCA 
CTGGACACTT CCCTCCTGCT AATCAAGGAA GCTCTCCAAC TGGTGGAAGA GCATGTGGGT 
GCCTGCTACC TGGCCCAACC CTTTTTGGCA CTACTCTCCC GCCTTCCACT GGAGTCTTAC 
CCCGAGCATG TGCATCAGCA CCACAAGGAT GCCACCGAAC TGGCGGAGAA GCTAGCTGCG 
CAGAAAATGA AACCCTTGGC TCCGGCGGAA AAGAAACCCA AGGCTCTTAG GCTGCTGGAA 
CCTCGATTCG AGGCGGTTTA CGATGACAAA CGTCGTCCCA AGATGTCCAA GGCCAAGGAA 
GAGCGGGCCA AACTGCTGCA CAAGATCAAG CGCGAGAAGA AGGGTGCCAT TCGAGAAATA 
CGCAGAGATA CGTCCTTCGT TCAGATGCTT AGGCTCAAAC AGACCCTGCA AAGTGACAAG 
GAGCGCCACG AGAAGGTTAA GCGCATTTAC CAGGAGGCAT CCGTGCAACA GGGCGAGCTC 
AATGAGCTGT CCCGGGCCAA AAAGAAGAAG AAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(3)07882 ( NM_143783.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652040.2 Drosophila melanogaster lethal (3) 07882 (l(3)07882), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06911
Clone ID Related Accession (Same CDS sequence)	NM_001316506.1 , NM_143783.3
Accession Version	NM_143783.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (3) 07882, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143783.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGCCA AAGGAAAGAA GGCCAGCGCA GATGCGGTTT ACGCCAAAAA GACAACCAGG 
TCGGCCAATC CCTTCGACAA CTCGACGGCA CAGTCCAGCA AGCGGGGCAA TCCCTTCGAT 
GTGCACGTGA ACAAGGAGAA GTTCAAGATT CTGGGGAGGA TCTGCAAGCA TGACAGGGGA 
ATGCCGGGCG TGTCGCGGGC CAAGGCTCTG CAGAAACGAG CGCAGACATT GGGTCAACAG 
TTTGCCGTGA AGCACAAGAC CAATCGCTTC AAGGATAACC GAATTGGAAA ACACTTGAGT 
GGCGATCAGC TAACCGAGTC CGTGATGAAT GCCCGCTACC TGGCCGAAAA GATGTCCCAG 
GTGCGCTCCA ACCAGAAAGC GGAGAAGTTC AACCTCAACG ACGACGAACT ACTGACGCAC 
AGGGGACAAA CTCTGGAGGA GATCGAGCAG TACAGGGACG AGCGCTCCGA CGACGAGGAG 
CTAGACGACG AGGCATTAGA TGCCGATTTT ACTGCTGCTG CTCATTTTGG CGGAGAGGGA 
GACTCTGCCC AGGATAGACA GGCGGCCATC GATGAGATGA TCGTTGAACA AAAACGAAGA 
AAGAACGAAA TCGCCAAGGA GAAGGACGAA GTGTATGATC TCACAGAGAA ACTGGATGCC 
AACTATAAGC TTCTGCTGCC GCTGGTCGCC AAGGTCACGA AGGATGAGCA GGATGCCAAG 
CCACCGCCAG ATGCCTACGA TAAACTGCTC AAGGAGATGA TCTTCGAGCC GCGTGGCAGT 
GTCGCGGATA AACTAATCAA TCCCGACGAG CTGGCCAAAC AGGAGGCCGC TCGATTGGAG 
AAGCTCGAGA ACGAACGGCT TCGTCGCATG CGAGCAGATG GCGAGGAGGA TGAGGAGGCC 
TCCGTTGCCA AACCCAAGCA CCGATCCGCA GATGACCTGG ATGATGGCTA TTTCTTGGCC 
GGCGAAGATG ACGAGGGAGA TGATACGCTC GCTTACGATT TGGACGGCAA TCTGGGCACA 
CATCTCAATG GAAAGAAGGA AGCCGTGTTG AAGGGAGACG AAAATGAGGA TGACGATGAC 
AAGGAGGGGG AAGAAGAGGA AGAGGAGGAC TCGGATGAGG AGAGTGATTC AGAGGTCGAC 
AATCTGAGCG ATCTCAAGGA ATCTGAAAGT GAGTCAGAGC CAGAAGAAGC TCCGAAGGCT 
AGCAAAAAGA AAGCGAAAAA GTCTGCGGAT ACAATTCCCG CCTCCTTGGA CACCAGTATT 
CCGTTTACCA TCAAGATGCC CAAGACCTAC GAGGACTTTA CGGAGCTGCT CTCCAAGCAT 
GCTACGGCCC AGAAGGCGAT CATTATCGAA AGGATTATAA AATGTAATCA TCCCAAACTG 
GAAGGTGTTA ACCGCGAGAA CGTGGTTAAG TTGTACTCAT TTTTGCTGCA ATATCTGAAG 
GATTTGTTTG AAGATGCCAG CGAGCAGGAC ATACGAGAAC ATTTCCAGTT GCTCTCAAAG 
CTCATGCCTT ACTTATATGA ATTAACGCAA CTGAATCCCG AGCGCATGTC CAATACATTA 
CTGGAGGTGA TCAAGGAGAA GTACGAAGAG TTCAGGAAAA ATCACAAAAT GTATCCTTCA 
CTTGACACTT TGGTGTACTT CAAACTAGTG GCCAACTTGT ACTCGACTTC CGATTTCCGC 
CATCCGGTGG TCACTCCTTG CTTTATATTC ATACAACACG TCCTGTCCAG ATCACGAGTG 
CGAACGCGTC AGGAAATATC TATGGGTTTG TTCCTGGTCA CCGTAGTCTT GGAGTTTGTG 
TCCCAATCCA AGAGACTGGT TCCAGCCGTA TTCAACTTCC TGCAGGGCAT TGTACACATG 
TCCATACCGA AGAGGGATGT GGAACAGTTG GAGATCACGC CGCCTTTTGA GCGTGATGGT 
CCATTGAGCA AGCTCCTAGC ACTTCCAGCT AACACGGAAT CCACGAAGTT GGAGCCCCAA 
CAGCTGCAGC CTACTGACTT GGTCACCCAG ACGATCACTC CAGATTTCAA GGTTCGAGCA 
CTGGACACTT CCCTCCTGCT AATCAAGGAA GCTCTCCAAC TGGTGGAAGA GCATGTGGGT 
GCCTGCTACC TGGCCCAACC CTTTTTGGCA CTACTCTCCC GCCTTCCACT GGAGTCTTAC 
CCCGAGCATG TGCATCAGCA CCACAAGGAT GCCACCGAAC TGGCGGAGAA GCTAGCTGCG 
CAGAAAATGA AACCCTTGGC TCCGGCGGAA AAGAAACCCA AGGCTCTTAG GCTGCTGGAA 
CCTCGATTCG AGGCGGTTTA CGATGACAAA CGTCGTCCCA AGATGTCCAA GGCCAAGGAA 
GAGCGGGCCA AACTGCTGCA CAAGATCAAG CGCGAGAAGA AGGGTGCCAT TCGAGAAATA 
CGCAGAGATA CGTCCTTCGT TCAGATGCTT AGGCTCAAAC AGACCCTGCA AAGTGACAAG 
GAGCGCCACG AGAAGGTTAA GCGCATTTAC CAGGAGGCAT CCGTGCAACA GGGCGAGCTC 
AATGAGCTGT CCCGGGCCAA AAAGAAGAAG AAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652040.2
CDS	101..2659
Translation	MVAKGKKASADAVYAKKTTRSANPFDNSTAQSSKRGNPFDVHVNKEKFKILGRICKHDRGMPGVSRAKALQKRAQTLGQQFAVKHKTNRFKDNRIGKHLSGDQLTESVMNARYLAEKMSQVRSNQKAEKFNLNDDELLTHRGQTLEEIEQYRDERSDDEELDDEALDADFTAAAHFGGEGDSAQDRQAAIDEMIVEQKRRKNEIAKEKDEVYDLTEKLDANYKLLLPLVAKVTKDEQDAKPPPDAYDKLLKEMIFEPRGSVADKLINPDELAKQEAARLEKLENERLRRMRADGEEDEEASVAKPKHRSADDLDDGYFLAGEDDEGDDTLAYDLDGNLGTHLNGKKEAVLKGDENEDDDDKEGEEEEEEDSDEESDSEVDNLSDLKESESESEPEEAPKASKKKAKKSADTIPASLDTSIPFTIKMPKTYEDFTELLSKHATAQKAIIIERIIKCNHPKLEGVNRENVVKLYSFLLQYLKDLFEDASEQDIREHFQLLSKLMPYLYELTQLNPERMSNTLLEVIKEKYEEFRKNHKMYPSLDTLVYFKLVANLYSTSDFRHPVVTPCFIFIQHVLSRSRVRTRQEISMGLFLVTVVLEFVSQSKRLVPAVFNFLQGIVHMSIPKRDVEQLEITPPFERDGPLSKLLALPANTESTKLEPQQLQPTDLVTQTITPDFKVRALDTSLLLIKEALQLVEEHVGACYLAQPFLALLSRLPLESYPEHVHQHHKDATELAEKLAAQKMKPLAPAEKKPKALRLLEPRFEAVYDDKRRPKMSKAKEERAKLLHKIKREKKGAIREIRRDTSFVQMLRLKQTLQSDKERHEKVKRIYQEASVQQGELNELSRAKKKKKF

Target ORF information:

RefSeq Version	NM_143783.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (3) 07882 (l(3)07882), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143783.3

ORF Insert Sequence:

ATGGTGGCCA AAGGAAAGAA GGCCAGCGCA GATGCGGTTT ACGCCAAAAA GACAACCAGG 
TCGGCCAATC CCTTCGACAA CTCGACGGCA CAGTCCAGCA AGCGGGGCAA TCCCTTCGAT 
GTGCACGTGA ACAAGGAGAA GTTCAAGATT CTGGGGAGGA TCTGCAAGCA TGACAGGGGA 
ATGCCGGGCG TGTCGCGGGC CAAGGCTCTG CAGAAACGAG CGCAGACATT GGGTCAACAG 
TTTGCCGTGA AGCACAAGAC CAATCGCTTC AAGGATAACC GAATTGGAAA ACACTTGAGT 
GGCGATCAGC TAACCGAGTC CGTGATGAAT GCCCGCTACC TGGCCGAAAA GATGTCCCAG 
GTGCGCTCCA ACCAGAAAGC GGAGAAGTTC AACCTCAACG ACGACGAACT ACTGACGCAC 
AGGGGACAAA CTCTGGAGGA GATCGAGCAG TACAGGGACG AGCGCTCCGA CGACGAGGAG 
CTAGACGACG AGGCATTAGA TGCCGATTTT ACTGCTGCTG CTCATTTTGG CGGAGAGGGA 
GACTCTGCCC AGGATAGACA GGCGGCCATC GATGAGATGA TCGTTGAACA AAAACGAAGA 
AAGAACGAAA TCGCCAAGGA GAAGGACGAA GTGTATGATC TCACAGAGAA ACTGGATGCC 
AACTATAAGC TTCTGCTGCC GCTGGTCGCC AAGGTCACGA AGGATGAGCA GGATGCCAAG 
CCACCGCCAG ATGCCTACGA TAAACTGCTC AAGGAGATGA TCTTCGAGCC GCGTGGCAGT 
GTCGCGGATA AACTAATCAA TCCCGACGAG CTGGCCAAAC AGGAGGCCGC TCGATTGGAG 
AAGCTCGAGA ACGAACGGCT TCGTCGCATG CGAGCAGATG GCGAGGAGGA TGAGGAGGCC 
TCCGTTGCCA AACCCAAGCA CCGATCCGCA GATGACCTGG ATGATGGCTA TTTCTTGGCC 
GGCGAAGATG ACGAGGGAGA TGATACGCTC GCTTACGATT TGGACGGCAA TCTGGGCACA 
CATCTCAATG GAAAGAAGGA AGCCGTGTTG AAGGGAGACG AAAATGAGGA TGACGATGAC 
AAGGAGGGGG AAGAAGAGGA AGAGGAGGAC TCGGATGAGG AGAGTGATTC AGAGGTCGAC 
AATCTGAGCG ATCTCAAGGA ATCTGAAAGT GAGTCAGAGC CAGAAGAAGC TCCGAAGGCT 
AGCAAAAAGA AAGCGAAAAA GTCTGCGGAT ACAATTCCCG CCTCCTTGGA CACCAGTATT 
CCGTTTACCA TCAAGATGCC CAAGACCTAC GAGGACTTTA CGGAGCTGCT CTCCAAGCAT 
GCTACGGCCC AGAAGGCGAT CATTATCGAA AGGATTATAA AATGTAATCA TCCCAAACTG 
GAAGGTGTTA ACCGCGAGAA CGTGGTTAAG TTGTACTCAT TTTTGCTGCA ATATCTGAAG 
GATTTGTTTG AAGATGCCAG CGAGCAGGAC ATACGAGAAC ATTTCCAGTT GCTCTCAAAG 
CTCATGCCTT ACTTATATGA ATTAACGCAA CTGAATCCCG AGCGCATGTC CAATACATTA 
CTGGAGGTGA TCAAGGAGAA GTACGAAGAG TTCAGGAAAA ATCACAAAAT GTATCCTTCA 
CTTGACACTT TGGTGTACTT CAAACTAGTG GCCAACTTGT ACTCGACTTC CGATTTCCGC 
CATCCGGTGG TCACTCCTTG CTTTATATTC ATACAACACG TCCTGTCCAG ATCACGAGTG 
CGAACGCGTC AGGAAATATC TATGGGTTTG TTCCTGGTCA CCGTAGTCTT GGAGTTTGTG 
TCCCAATCCA AGAGACTGGT TCCAGCCGTA TTCAACTTCC TGCAGGGCAT TGTACACATG 
TCCATACCGA AGAGGGATGT GGAACAGTTG GAGATCACGC CGCCTTTTGA GCGTGATGGT 
CCATTGAGCA AGCTCCTAGC ACTTCCAGCT AACACGGAAT CCACGAAGTT GGAGCCCCAA 
CAGCTGCAGC CTACTGACTT GGTCACCCAG ACGATCACTC CAGATTTCAA GGTTCGAGCA 
CTGGACACTT CCCTCCTGCT AATCAAGGAA GCTCTCCAAC TGGTGGAAGA GCATGTGGGT 
GCCTGCTACC TGGCCCAACC CTTTTTGGCA CTACTCTCCC GCCTTCCACT GGAGTCTTAC 
CCCGAGCATG TGCATCAGCA CCACAAGGAT GCCACCGAAC TGGCGGAGAA GCTAGCTGCG 
CAGAAAATGA AACCCTTGGC TCCGGCGGAA AAGAAACCCA AGGCTCTTAG GCTGCTGGAA 
CCTCGATTCG AGGCGGTTTA CGATGACAAA CGTCGTCCCA AGATGTCCAA GGCCAAGGAA 
GAGCGGGCCA AACTGCTGCA CAAGATCAAG CGCGAGAAGA AGGGTGCCAT TCGAGAAATA 
CGCAGAGATA CGTCCTTCGT TCAGATGCTT AGGCTCAAAC AGACCCTGCA AAGTGACAAG 
GAGCGCCACG AGAAGGTTAA GCGCATTTAC CAGGAGGCAT CCGTGCAACA GGGCGAGCTC 
AATGAGCTGT CCCGGGCCAA AAAGAAGAAG AAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(3)07882 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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