NAT1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAT1
Entrez Gene ID	46020
Full Name	CG3845 gene product from transcript CG3845-RA
Synonyms	CG3845,Dmel\CG3845,Nat1,dNAT1,l(2)01424
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49E1-49E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165961.2	NP_725242.1	NAT1, isoform A
NM_143770.3	NP_652027.2	NAT1, isoform B
NM_001038854.2	NP_001033943.2	NAT1, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	NAT1	NP_001033943.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004750	XP_318067.4
Danio rerio (zebrafish)	eif4g2b	NP_001013461.2
Danio rerio (zebrafish)	eif4g2a	NP_001014311.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors conclude that PER expression, and perhaps circadian protein expression more broadly, relies in part upon cap-independent translation and the translation factor NAT1.
Title: NAT1/DAP5/p97 and atypical translational control in the Drosophila Circadian Oscillator.

The following NAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12725	NM_165961.2 Latest version!	Drosophila melanogaster NAT1, transcript variant A (NAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12725	NM_143770.3 Latest version!	Drosophila melanogaster NAT1, transcript variant B (NAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12726	NM_001038854.2 Latest version!	Drosophila melanogaster NAT1, transcript variant D (NAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAT1 ( NM_165961.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725242.1 Drosophila melanogaster NAT1, transcript variant A (NAT1), mRNA.

Price & Availability↑

CloneID	OFa12725
Clone ID Related Accession (Same CDS sequence)	NM_165961.2 , NM_143770.3
Accession Version	NM_165961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NAT1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165961.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATGCGC AGCTGTGCAA GCGACTTTCC GAGGAAGCCC CGTCATTCGA CAAGGAACCA 
AGCAATTCGT CCACCTTCTT ACGCCTGCTG ATCGCGGTCT GCCGCGACAA GTTCAACAAC 
CGACTCAAGC GCGACGAGAA CGACAACAGA CCTCCGCCTG AAAATGAGGC CGACGAGGAG 
GAGCGCCGCC ATTTGGCCAA ACAGCGCATG CTCGGCAACG TCAAATTCAT CGGAGAGCTC 
AACAAGCTGG ACATGCTGTC GAAGAACGTG CTGCATCAGT GCATCATGGA GCTATTTGAC 
AAGAAGAAGA AGCGTACGGC CGGTACGCAG GAAATGTGCG AGGACATGGA ATGCCTGGCT 
CAACTGCTCA AGACTTGCGG CAAGAATCTG GATTCAGAAC AGGGCAAAGA GCTGATGAAC 
CAGTACTTCG AGAAGCTTGA ACGTCGGTCA AAATCATCTG AATATCCACC GCGGATACGT 
TTTATGCTAA AGGACGTAAT TGAATTGCGC CAGAACAATT GGGTGCCGCG CAAATTGGGC 
ACCACCGAAG GCCCCGTCCC CATAAAGCAA ATACGCTCGG ATGACGACTC AATCATACGC 
ACTCCGTTCC CCAATCGCAA TCGTGATATG CGCAACAATG ATCGTGACTC GGACAGCTGG 
ATGAATCGTT TCCATCTGAA TATCCAGCCT GGCGGCTATA ATGACATGTT TAGCGGACTG 
AGTGTTACGG GAGCTTCTCC GATTGTCTCG CCGTTCGCTA CGAGCAATCG TGACCGAGAC 
CGCGACAATC GCCAGTACAA CAATCGAGGC GATCGCAACA GGGATCGCGA TCAGGGCGGT 
AGCGGCGGAG CTAACTATGG CAATCGCTAC AACAAGCACA ACCAGAATGG AGGAGGCAGT 
GGCGGAGGAT CCTCCAACAA CCGGGACCGG GATGATTCGC GTAGGAATAA CGATCGTGAC 
AGGGATCGCA ATGACGGACA GTATGGGGGC AATCAGCTGC TGGGTAGTAA GGAATTGGCA 
CCGAGGTTCA AACGCAACTT GATCACCACG AACCAGGATG CGGTGGAGAA CTTGCAAATG 
CGCCCGGCAG CCAACTCCTT GCTCTTCCGA GCGGCCTCCC AGAACCAAAA GTTTCCGGCT 
ACGTTGCCCA TGTCAACACC GCCCAATAGC AGCGGCAGCA ACAACCAGGG CAGCTCGCAG 
GGCCAGCTCT CGAACTCCCA CTACCCTATG CTCGGCACGC CCACATCCCA TCTGATCAAT 
CCAACGCGTC CCTCGTCAAC ACCATCTGCT GAGTATGCTG ATAACCGAAC TCTTTTGGGA 
GTTCAACAGG AGAGGGGATT GAAGGCCACC TCTTCTTCGC CGAACTTTTC ATCAGGAGCG 
GAGAAGTTGG ATTTAGACGG TCAGGCGGGC AGAAAGGGAT CGCACGACGG CAAAGATCAG 
GGCAAGAATG GCTCCGTGGA GAGACCAAGC ACTCCGGCCG GCGGTATTGG CTCGGCCAAG 
CAACAAAAGA AAGATAAGGG TCCCAACAAG GAGGAGCTGT CTAAGAAGGC CAGTCAGTAC 
TTCAAGGATA GGTTCTACTA CGATGAGGAG GAGGATGCAG AGGTGACGGA AATTGAGATC 
AAGGATATTG ATGAGACAAA GACGGAGGAG GCCGACGCTG CTCCAGTTGA AGTCTCGGAG 
GTAAAGGAAG ATGCTGCAAT ACCTGTGACC ACTCCAGATC GCTTCACCAA GCTTGTAGAT 
GGTTTCCTCG AGCTTAAGCT GCCAGAGAAG GCGCTGAAGG ATGTGTGCAT CAATCTGCTG 
ATGGAGGTCT TGGACCGTGT AAACGATGTC TTCTTGGAGC GTGCTGTGCG CCTTCTGCAG 
ACATTGCGCA AGCAATCCAA TATCAAGCCG AACATCGTGG TCGAGATTTT CAAGCAGGTG 
GTCAACAAGA TGAACGAGCG AGAGGCACTC AATCCCCGAA TCGTTAGTCT TGTAGCCAGC 
TTGCTGGCAA AGACAATTTG CGAGCCAGCA TTGCTGAAGT TAAGCGATAT CGCCAACTTC 
ACGGACAATG GCCAACACTA TCCGCTGTTC CTGCTCGTGC TGCAGCAACT GGTCAAGGTC 
ATCGGCAAGG ATGCGCTGGA AGAGAAATTC CGGGCGAGCA AAGTTGATCT TATGAACAGC 
CTGCCGGAGG CGGATCGGAA CAAGGAGCGC TTGGCCGGGA TACTCGAGGA TCGCAAGCTT 
TCTTTCCTTT ATCCGCTATT GAAGGTACAG GCCGAGATGC TAAAACAGCT GCAAAGCGAC 
CCGAACCCCA ACAACTTCTA CAAGTGGATC AAGGCCAACG TTGACAACAA ATACTACAAG 
GACCCGGGCT TTATCCAAGC CCTAATGACC GTGGTGGTCA AGTATGTGAC CAAGGAGACG 
ACGCTGGCGG ACAACCTCGA CCCCAAGGAG CACCCTGAGA AATCGGTGAC CCAGAAGGAG 
CAACAGCTGC TGGAGAACTA CAGCCAGATG CTGCAGACCT TCCTGGGACA GAACGAGCTT 
CAGTTGATGG CTCTCTATGC TCTGCAAACG TTCTGCTACA ATGAAAATTT CCCCAAGGGC 
ATGCTGTGCC GTTGGTTCAA GTATCTTTAC GAATCTGAAA TTATAGAGGA GGAGGCATTC 
GTCCTGTGGA AGGAGGAGAT TTCCGACAAA TATCCAGGCA AAGGATCCGC TTTGTTCCAA 
GTGAACGCCT GGTTGACTTG GCTGCAGGAG GCCGAATCTG AGGACGATGA GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725242.1
CDS	2185..4941
Translation	MYAQLCKRLSEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLKRDENDNRPPPENEADEEERRHLAKQRMLGNVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWVPRKLGTTEGPVPIKQIRSDDDSIIRTPFPNRNRDMRNNDRDSDSWMNRFHLNIQPGGYNDMFSGLSVTGASPIVSPFATSNRDRDRDNRQYNNRGDRNRDRDQGGSGGANYGNRYNKHNQNGGGSGGGSSNNRDRDDSRRNNDRDRDRNDGQYGGNQLLGSKELAPRFKRNLITTNQDAVENLQMRPAANSLLFRAASQNQKFPATLPMSTPPNSSGSNNQGSSQGQLSNSHYPMLGTPTSHLINPTRPSSTPSAEYADNRTLLGVQQERGLKATSSSPNFSSGAEKLDLDGQAGRKGSHDGKDQGKNGSVERPSTPAGGIGSAKQQKKDKGPNKEELSKKASQYFKDRFYYDEEEDAEVTEIEIKDIDETKTEEADAAPVEVSEVKEDAAIPVTTPDRFTKLVDGFLELKLPEKALKDVCINLLMEVLDRVNDVFLERAVRLLQTLRKQSNIKPNIVVEIFKQVVNKMNEREALNPRIVSLVASLLAKTICEPALLKLSDIANFTDNGQHYPLFLLVLQQLVKVIGKDALEEKFRASKVDLMNSLPEADRNKERLAGILEDRKLSFLYPLLKVQAEMLKQLQSDPNPNNFYKWIKANVDNKYYKDPGFIQALMTVVVKYVTKETTLADNLDPKEHPEKSVTQKEQQLLENYSQMLQTFLGQNELQLMALYALQTFCYNENFPKGMLCRWFKYLYESEIIEEEAFVLWKEEISDKYPGKGSALFQVNAWLTWLQEAESEDDED

Target ORF information:

RefSeq Version	NM_165961.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NAT1, transcript variant A (NAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165961.2

ORF Insert Sequence:

ATGTATGCGC AGCTGTGCAA GCGACTTTCC GAGGAAGCCC CGTCATTCGA CAAGGAACCA 
AGCAATTCGT CCACCTTCTT ACGCCTGCTG ATCGCGGTCT GCCGCGACAA GTTCAACAAC 
CGACTCAAGC GCGACGAGAA CGACAACAGA CCTCCGCCTG AAAATGAGGC CGACGAGGAG 
GAGCGCCGCC ATTTGGCCAA ACAGCGCATG CTCGGCAACG TCAAATTCAT CGGAGAGCTC 
AACAAGCTGG ACATGCTGTC GAAGAACGTG CTGCATCAGT GCATCATGGA GCTATTTGAC 
AAGAAGAAGA AGCGTACGGC CGGTACGCAG GAAATGTGCG AGGACATGGA ATGCCTGGCT 
CAACTGCTCA AGACTTGCGG CAAGAATCTG GATTCAGAAC AGGGCAAAGA GCTGATGAAC 
CAGTACTTCG AGAAGCTTGA ACGTCGGTCA AAATCATCTG AATATCCACC GCGGATACGT 
TTTATGCTAA AGGACGTAAT TGAATTGCGC CAGAACAATT GGGTGCCGCG CAAATTGGGC 
ACCACCGAAG GCCCCGTCCC CATAAAGCAA ATACGCTCGG ATGACGACTC AATCATACGC 
ACTCCGTTCC CCAATCGCAA TCGTGATATG CGCAACAATG ATCGTGACTC GGACAGCTGG 
ATGAATCGTT TCCATCTGAA TATCCAGCCT GGCGGCTATA ATGACATGTT TAGCGGACTG 
AGTGTTACGG GAGCTTCTCC GATTGTCTCG CCGTTCGCTA CGAGCAATCG TGACCGAGAC 
CGCGACAATC GCCAGTACAA CAATCGAGGC GATCGCAACA GGGATCGCGA TCAGGGCGGT 
AGCGGCGGAG CTAACTATGG CAATCGCTAC AACAAGCACA ACCAGAATGG AGGAGGCAGT 
GGCGGAGGAT CCTCCAACAA CCGGGACCGG GATGATTCGC GTAGGAATAA CGATCGTGAC 
AGGGATCGCA ATGACGGACA GTATGGGGGC AATCAGCTGC TGGGTAGTAA GGAATTGGCA 
CCGAGGTTCA AACGCAACTT GATCACCACG AACCAGGATG CGGTGGAGAA CTTGCAAATG 
CGCCCGGCAG CCAACTCCTT GCTCTTCCGA GCGGCCTCCC AGAACCAAAA GTTTCCGGCT 
ACGTTGCCCA TGTCAACACC GCCCAATAGC AGCGGCAGCA ACAACCAGGG CAGCTCGCAG 
GGCCAGCTCT CGAACTCCCA CTACCCTATG CTCGGCACGC CCACATCCCA TCTGATCAAT 
CCAACGCGTC CCTCGTCAAC ACCATCTGCT GAGTATGCTG ATAACCGAAC TCTTTTGGGA 
GTTCAACAGG AGAGGGGATT GAAGGCCACC TCTTCTTCGC CGAACTTTTC ATCAGGAGCG 
GAGAAGTTGG ATTTAGACGG TCAGGCGGGC AGAAAGGGAT CGCACGACGG CAAAGATCAG 
GGCAAGAATG GCTCCGTGGA GAGACCAAGC ACTCCGGCCG GCGGTATTGG CTCGGCCAAG 
CAACAAAAGA AAGATAAGGG TCCCAACAAG GAGGAGCTGT CTAAGAAGGC CAGTCAGTAC 
TTCAAGGATA GGTTCTACTA CGATGAGGAG GAGGATGCAG AGGTGACGGA AATTGAGATC 
AAGGATATTG ATGAGACAAA GACGGAGGAG GCCGACGCTG CTCCAGTTGA AGTCTCGGAG 
GTAAAGGAAG ATGCTGCAAT ACCTGTGACC ACTCCAGATC GCTTCACCAA GCTTGTAGAT 
GGTTTCCTCG AGCTTAAGCT GCCAGAGAAG GCGCTGAAGG ATGTGTGCAT CAATCTGCTG 
ATGGAGGTCT TGGACCGTGT AAACGATGTC TTCTTGGAGC GTGCTGTGCG CCTTCTGCAG 
ACATTGCGCA AGCAATCCAA TATCAAGCCG AACATCGTGG TCGAGATTTT CAAGCAGGTG 
GTCAACAAGA TGAACGAGCG AGAGGCACTC AATCCCCGAA TCGTTAGTCT TGTAGCCAGC 
TTGCTGGCAA AGACAATTTG CGAGCCAGCA TTGCTGAAGT TAAGCGATAT CGCCAACTTC 
ACGGACAATG GCCAACACTA TCCGCTGTTC CTGCTCGTGC TGCAGCAACT GGTCAAGGTC 
ATCGGCAAGG ATGCGCTGGA AGAGAAATTC CGGGCGAGCA AAGTTGATCT TATGAACAGC 
CTGCCGGAGG CGGATCGGAA CAAGGAGCGC TTGGCCGGGA TACTCGAGGA TCGCAAGCTT 
TCTTTCCTTT ATCCGCTATT GAAGGTACAG GCCGAGATGC TAAAACAGCT GCAAAGCGAC 
CCGAACCCCA ACAACTTCTA CAAGTGGATC AAGGCCAACG TTGACAACAA ATACTACAAG 
GACCCGGGCT TTATCCAAGC CCTAATGACC GTGGTGGTCA AGTATGTGAC CAAGGAGACG 
ACGCTGGCGG ACAACCTCGA CCCCAAGGAG CACCCTGAGA AATCGGTGAC CCAGAAGGAG 
CAACAGCTGC TGGAGAACTA CAGCCAGATG CTGCAGACCT TCCTGGGACA GAACGAGCTT 
CAGTTGATGG CTCTCTATGC TCTGCAAACG TTCTGCTACA ATGAAAATTT CCCCAAGGGC 
ATGCTGTGCC GTTGGTTCAA GTATCTTTAC GAATCTGAAA TTATAGAGGA GGAGGCATTC 
GTCCTGTGGA AGGAGGAGAT TTCCGACAAA TATCCAGGCA AAGGATCCGC TTTGTTCCAA 
GTGAACGCCT GGTTGACTTG GCTGCAGGAG GCCGAATCTG AGGACGATGA GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAT1 ( NM_143770.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652027.2 Drosophila melanogaster NAT1, transcript variant B (NAT1), mRNA.

Price & Availability↑

CloneID	OFa12725
Clone ID Related Accession (Same CDS sequence)	NM_165961.2 , NM_143770.3
Accession Version	NM_143770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NAT1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_143770.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATGCGC AGCTGTGCAA GCGACTTTCC GAGGAAGCCC CGTCATTCGA CAAGGAACCA 
AGCAATTCGT CCACCTTCTT ACGCCTGCTG ATCGCGGTCT GCCGCGACAA GTTCAACAAC 
CGACTCAAGC GCGACGAGAA CGACAACAGA CCTCCGCCTG AAAATGAGGC CGACGAGGAG 
GAGCGCCGCC ATTTGGCCAA ACAGCGCATG CTCGGCAACG TCAAATTCAT CGGAGAGCTC 
AACAAGCTGG ACATGCTGTC GAAGAACGTG CTGCATCAGT GCATCATGGA GCTATTTGAC 
AAGAAGAAGA AGCGTACGGC CGGTACGCAG GAAATGTGCG AGGACATGGA ATGCCTGGCT 
CAACTGCTCA AGACTTGCGG CAAGAATCTG GATTCAGAAC AGGGCAAAGA GCTGATGAAC 
CAGTACTTCG AGAAGCTTGA ACGTCGGTCA AAATCATCTG AATATCCACC GCGGATACGT 
TTTATGCTAA AGGACGTAAT TGAATTGCGC CAGAACAATT GGGTGCCGCG CAAATTGGGC 
ACCACCGAAG GCCCCGTCCC CATAAAGCAA ATACGCTCGG ATGACGACTC AATCATACGC 
ACTCCGTTCC CCAATCGCAA TCGTGATATG CGCAACAATG ATCGTGACTC GGACAGCTGG 
ATGAATCGTT TCCATCTGAA TATCCAGCCT GGCGGCTATA ATGACATGTT TAGCGGACTG 
AGTGTTACGG GAGCTTCTCC GATTGTCTCG CCGTTCGCTA CGAGCAATCG TGACCGAGAC 
CGCGACAATC GCCAGTACAA CAATCGAGGC GATCGCAACA GGGATCGCGA TCAGGGCGGT 
AGCGGCGGAG CTAACTATGG CAATCGCTAC AACAAGCACA ACCAGAATGG AGGAGGCAGT 
GGCGGAGGAT CCTCCAACAA CCGGGACCGG GATGATTCGC GTAGGAATAA CGATCGTGAC 
AGGGATCGCA ATGACGGACA GTATGGGGGC AATCAGCTGC TGGGTAGTAA GGAATTGGCA 
CCGAGGTTCA AACGCAACTT GATCACCACG AACCAGGATG CGGTGGAGAA CTTGCAAATG 
CGCCCGGCAG CCAACTCCTT GCTCTTCCGA GCGGCCTCCC AGAACCAAAA GTTTCCGGCT 
ACGTTGCCCA TGTCAACACC GCCCAATAGC AGCGGCAGCA ACAACCAGGG CAGCTCGCAG 
GGCCAGCTCT CGAACTCCCA CTACCCTATG CTCGGCACGC CCACATCCCA TCTGATCAAT 
CCAACGCGTC CCTCGTCAAC ACCATCTGCT GAGTATGCTG ATAACCGAAC TCTTTTGGGA 
GTTCAACAGG AGAGGGGATT GAAGGCCACC TCTTCTTCGC CGAACTTTTC ATCAGGAGCG 
GAGAAGTTGG ATTTAGACGG TCAGGCGGGC AGAAAGGGAT CGCACGACGG CAAAGATCAG 
GGCAAGAATG GCTCCGTGGA GAGACCAAGC ACTCCGGCCG GCGGTATTGG CTCGGCCAAG 
CAACAAAAGA AAGATAAGGG TCCCAACAAG GAGGAGCTGT CTAAGAAGGC CAGTCAGTAC 
TTCAAGGATA GGTTCTACTA CGATGAGGAG GAGGATGCAG AGGTGACGGA AATTGAGATC 
AAGGATATTG ATGAGACAAA GACGGAGGAG GCCGACGCTG CTCCAGTTGA AGTCTCGGAG 
GTAAAGGAAG ATGCTGCAAT ACCTGTGACC ACTCCAGATC GCTTCACCAA GCTTGTAGAT 
GGTTTCCTCG AGCTTAAGCT GCCAGAGAAG GCGCTGAAGG ATGTGTGCAT CAATCTGCTG 
ATGGAGGTCT TGGACCGTGT AAACGATGTC TTCTTGGAGC GTGCTGTGCG CCTTCTGCAG 
ACATTGCGCA AGCAATCCAA TATCAAGCCG AACATCGTGG TCGAGATTTT CAAGCAGGTG 
GTCAACAAGA TGAACGAGCG AGAGGCACTC AATCCCCGAA TCGTTAGTCT TGTAGCCAGC 
TTGCTGGCAA AGACAATTTG CGAGCCAGCA TTGCTGAAGT TAAGCGATAT CGCCAACTTC 
ACGGACAATG GCCAACACTA TCCGCTGTTC CTGCTCGTGC TGCAGCAACT GGTCAAGGTC 
ATCGGCAAGG ATGCGCTGGA AGAGAAATTC CGGGCGAGCA AAGTTGATCT TATGAACAGC 
CTGCCGGAGG CGGATCGGAA CAAGGAGCGC TTGGCCGGGA TACTCGAGGA TCGCAAGCTT 
TCTTTCCTTT ATCCGCTATT GAAGGTACAG GCCGAGATGC TAAAACAGCT GCAAAGCGAC 
CCGAACCCCA ACAACTTCTA CAAGTGGATC AAGGCCAACG TTGACAACAA ATACTACAAG 
GACCCGGGCT TTATCCAAGC CCTAATGACC GTGGTGGTCA AGTATGTGAC CAAGGAGACG 
ACGCTGGCGG ACAACCTCGA CCCCAAGGAG CACCCTGAGA AATCGGTGAC CCAGAAGGAG 
CAACAGCTGC TGGAGAACTA CAGCCAGATG CTGCAGACCT TCCTGGGACA GAACGAGCTT 
CAGTTGATGG CTCTCTATGC TCTGCAAACG TTCTGCTACA ATGAAAATTT CCCCAAGGGC 
ATGCTGTGCC GTTGGTTCAA GTATCTTTAC GAATCTGAAA TTATAGAGGA GGAGGCATTC 
GTCCTGTGGA AGGAGGAGAT TTCCGACAAA TATCCAGGCA AAGGATCCGC TTTGTTCCAA 
GTGAACGCCT GGTTGACTTG GCTGCAGGAG GCCGAATCTG AGGACGATGA GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652027.2
CDS	2161..4917
Translation	MYAQLCKRLSEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLKRDENDNRPPPENEADEEERRHLAKQRMLGNVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWVPRKLGTTEGPVPIKQIRSDDDSIIRTPFPNRNRDMRNNDRDSDSWMNRFHLNIQPGGYNDMFSGLSVTGASPIVSPFATSNRDRDRDNRQYNNRGDRNRDRDQGGSGGANYGNRYNKHNQNGGGSGGGSSNNRDRDDSRRNNDRDRDRNDGQYGGNQLLGSKELAPRFKRNLITTNQDAVENLQMRPAANSLLFRAASQNQKFPATLPMSTPPNSSGSNNQGSSQGQLSNSHYPMLGTPTSHLINPTRPSSTPSAEYADNRTLLGVQQERGLKATSSSPNFSSGAEKLDLDGQAGRKGSHDGKDQGKNGSVERPSTPAGGIGSAKQQKKDKGPNKEELSKKASQYFKDRFYYDEEEDAEVTEIEIKDIDETKTEEADAAPVEVSEVKEDAAIPVTTPDRFTKLVDGFLELKLPEKALKDVCINLLMEVLDRVNDVFLERAVRLLQTLRKQSNIKPNIVVEIFKQVVNKMNEREALNPRIVSLVASLLAKTICEPALLKLSDIANFTDNGQHYPLFLLVLQQLVKVIGKDALEEKFRASKVDLMNSLPEADRNKERLAGILEDRKLSFLYPLLKVQAEMLKQLQSDPNPNNFYKWIKANVDNKYYKDPGFIQALMTVVVKYVTKETTLADNLDPKEHPEKSVTQKEQQLLENYSQMLQTFLGQNELQLMALYALQTFCYNENFPKGMLCRWFKYLYESEIIEEEAFVLWKEEISDKYPGKGSALFQVNAWLTWLQEAESEDDED

Target ORF information:

RefSeq Version	NM_143770.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NAT1, transcript variant B (NAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143770.3

ORF Insert Sequence:

ATGTATGCGC AGCTGTGCAA GCGACTTTCC GAGGAAGCCC CGTCATTCGA CAAGGAACCA 
AGCAATTCGT CCACCTTCTT ACGCCTGCTG ATCGCGGTCT GCCGCGACAA GTTCAACAAC 
CGACTCAAGC GCGACGAGAA CGACAACAGA CCTCCGCCTG AAAATGAGGC CGACGAGGAG 
GAGCGCCGCC ATTTGGCCAA ACAGCGCATG CTCGGCAACG TCAAATTCAT CGGAGAGCTC 
AACAAGCTGG ACATGCTGTC GAAGAACGTG CTGCATCAGT GCATCATGGA GCTATTTGAC 
AAGAAGAAGA AGCGTACGGC CGGTACGCAG GAAATGTGCG AGGACATGGA ATGCCTGGCT 
CAACTGCTCA AGACTTGCGG CAAGAATCTG GATTCAGAAC AGGGCAAAGA GCTGATGAAC 
CAGTACTTCG AGAAGCTTGA ACGTCGGTCA AAATCATCTG AATATCCACC GCGGATACGT 
TTTATGCTAA AGGACGTAAT TGAATTGCGC CAGAACAATT GGGTGCCGCG CAAATTGGGC 
ACCACCGAAG GCCCCGTCCC CATAAAGCAA ATACGCTCGG ATGACGACTC AATCATACGC 
ACTCCGTTCC CCAATCGCAA TCGTGATATG CGCAACAATG ATCGTGACTC GGACAGCTGG 
ATGAATCGTT TCCATCTGAA TATCCAGCCT GGCGGCTATA ATGACATGTT TAGCGGACTG 
AGTGTTACGG GAGCTTCTCC GATTGTCTCG CCGTTCGCTA CGAGCAATCG TGACCGAGAC 
CGCGACAATC GCCAGTACAA CAATCGAGGC GATCGCAACA GGGATCGCGA TCAGGGCGGT 
AGCGGCGGAG CTAACTATGG CAATCGCTAC AACAAGCACA ACCAGAATGG AGGAGGCAGT 
GGCGGAGGAT CCTCCAACAA CCGGGACCGG GATGATTCGC GTAGGAATAA CGATCGTGAC 
AGGGATCGCA ATGACGGACA GTATGGGGGC AATCAGCTGC TGGGTAGTAA GGAATTGGCA 
CCGAGGTTCA AACGCAACTT GATCACCACG AACCAGGATG CGGTGGAGAA CTTGCAAATG 
CGCCCGGCAG CCAACTCCTT GCTCTTCCGA GCGGCCTCCC AGAACCAAAA GTTTCCGGCT 
ACGTTGCCCA TGTCAACACC GCCCAATAGC AGCGGCAGCA ACAACCAGGG CAGCTCGCAG 
GGCCAGCTCT CGAACTCCCA CTACCCTATG CTCGGCACGC CCACATCCCA TCTGATCAAT 
CCAACGCGTC CCTCGTCAAC ACCATCTGCT GAGTATGCTG ATAACCGAAC TCTTTTGGGA 
GTTCAACAGG AGAGGGGATT GAAGGCCACC TCTTCTTCGC CGAACTTTTC ATCAGGAGCG 
GAGAAGTTGG ATTTAGACGG TCAGGCGGGC AGAAAGGGAT CGCACGACGG CAAAGATCAG 
GGCAAGAATG GCTCCGTGGA GAGACCAAGC ACTCCGGCCG GCGGTATTGG CTCGGCCAAG 
CAACAAAAGA AAGATAAGGG TCCCAACAAG GAGGAGCTGT CTAAGAAGGC CAGTCAGTAC 
TTCAAGGATA GGTTCTACTA CGATGAGGAG GAGGATGCAG AGGTGACGGA AATTGAGATC 
AAGGATATTG ATGAGACAAA GACGGAGGAG GCCGACGCTG CTCCAGTTGA AGTCTCGGAG 
GTAAAGGAAG ATGCTGCAAT ACCTGTGACC ACTCCAGATC GCTTCACCAA GCTTGTAGAT 
GGTTTCCTCG AGCTTAAGCT GCCAGAGAAG GCGCTGAAGG ATGTGTGCAT CAATCTGCTG 
ATGGAGGTCT TGGACCGTGT AAACGATGTC TTCTTGGAGC GTGCTGTGCG CCTTCTGCAG 
ACATTGCGCA AGCAATCCAA TATCAAGCCG AACATCGTGG TCGAGATTTT CAAGCAGGTG 
GTCAACAAGA TGAACGAGCG AGAGGCACTC AATCCCCGAA TCGTTAGTCT TGTAGCCAGC 
TTGCTGGCAA AGACAATTTG CGAGCCAGCA TTGCTGAAGT TAAGCGATAT CGCCAACTTC 
ACGGACAATG GCCAACACTA TCCGCTGTTC CTGCTCGTGC TGCAGCAACT GGTCAAGGTC 
ATCGGCAAGG ATGCGCTGGA AGAGAAATTC CGGGCGAGCA AAGTTGATCT TATGAACAGC 
CTGCCGGAGG CGGATCGGAA CAAGGAGCGC TTGGCCGGGA TACTCGAGGA TCGCAAGCTT 
TCTTTCCTTT ATCCGCTATT GAAGGTACAG GCCGAGATGC TAAAACAGCT GCAAAGCGAC 
CCGAACCCCA ACAACTTCTA CAAGTGGATC AAGGCCAACG TTGACAACAA ATACTACAAG 
GACCCGGGCT TTATCCAAGC CCTAATGACC GTGGTGGTCA AGTATGTGAC CAAGGAGACG 
ACGCTGGCGG ACAACCTCGA CCCCAAGGAG CACCCTGAGA AATCGGTGAC CCAGAAGGAG 
CAACAGCTGC TGGAGAACTA CAGCCAGATG CTGCAGACCT TCCTGGGACA GAACGAGCTT 
CAGTTGATGG CTCTCTATGC TCTGCAAACG TTCTGCTACA ATGAAAATTT CCCCAAGGGC 
ATGCTGTGCC GTTGGTTCAA GTATCTTTAC GAATCTGAAA TTATAGAGGA GGAGGCATTC 
GTCCTGTGGA AGGAGGAGAT TTCCGACAAA TATCCAGGCA AAGGATCCGC TTTGTTCCAA 
GTGAACGCCT GGTTGACTTG GCTGCAGGAG GCCGAATCTG AGGACGATGA GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAT1 ( NM_001038854.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033943.2 Drosophila melanogaster NAT1, transcript variant D (NAT1), mRNA.

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CloneID	OFa12726
Clone ID Related Accession (Same CDS sequence)	NM_001038854.2
Accession Version	NM_001038854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NAT1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001038854.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATTCCTCTAT CAACGTACGA CGCCGCTCCC CTTTTGCACT CTTACGACGA CAGTCCGCAA 
CGTTGGTCCG GAGTCACCAA CGGGCACCAG GCAGGCGTCA TCATAGATCA GTTTCGGGGC 
GGTAAAGCGA TCATCATTGA GGGAGGCACC AGCACCGGTG ATACGAAGAG CATCGGCGGC 
GGCAGCGATT TACGACTGGT AATAACGGCC AATCCCAGCG AGATCGAGCG CAATTACGAG 
CAGCCAACCA ATCGCGGTGG TGGCGGAGGC GGCGGCGTTA TCGGCGTCAG CTGCGGTGGC 
CAAACCCCAC CGACACCGTC GGTCGGCAAA ACACTGCTCA ACACCAGTCC CAATCTTCTG 
CCGATCACCA CAGCGACCGA TACGTTTAAC AACCTCAAGA ATCTGGTGAA GATCGACAAG 
GGCTCCTCGC CATCGTCAGG ATCGAATTCG CTGGGCGGCA ATTACCGCAA GGGCGGCTCT 
AACGGCTCAG GATATAAGAG CGCCGGCGGC AACGGAGGAT CTCCGGCCAT AAGCGGCAAC 
AGCGAGGGCG GCAGGAACGG CAGCCGATCA TCTGGTGGAC CGACCGGACA CTTTTCCACT 
GGCTATAGTA AGAATTCCGT CAAAATTCGC CAACACGACA GTGAGGTGAG CCCAAGACCT 
CGACGATCTT ACCAGGGTGA CTCGTCGTCG TCGCGTTACT ATCCAAAGAA TAGTGACATT 
TTGGGTCAAG GCTTAGGCGG CAACTCCGAC AGCTACAACG GCAGTTCCTC TTCTGGTAGA 
TACCAGCAGC AGGATCAGCA AGGCGGATCG CATCGTGGTA GTCGCAGCAA CTATCACTCA 
AACTCCGATG GCAGCTACAA TCGATCAATA AACTCAAACT CCGTGGGAGG AGGAGGGTCT 
GGGGCAGTCG GCTCTGGAAG CGGTGGCAGC AATTATCGCT CCGCCCGCAA CTACGAGAAC 
GGCAATGGGA GCAACGGCAC ACAGCCACGT TACGGCAGCG GCAACGGGAA CGGCAACAGC 
AGCAATTCCA ACTCTTCATC CGGTTACAAC GGATCTGCAG CAAGCTCAAC CAACAGCAAG 
TCGTCGTATG GGGCTTCGCG CGGTGGCAAC GATGGAGGCG TGAGTAGCGG ATACAGCAGC 
AACTACCGCG ACAACTACGA GGATTCACCA TCGCACCAGC GCTACAACAA CACCAACAGC 
CTAGAGCGAA ACGGAGGAGG CGGATCAGGG GCAGGAGCGA GCGGAGGACC CCGCAGCTAC 
GGCAGGTCTG ACGCAGATCG TTACGGCGGA ACCCCGTCCA GTCGCACACC CAATCTTCGC 
AACGGCAGTG CGTCTGCTCA GATCTCTGGC TCCACTTCCA ATTCTTCGAA CATTTCTCTG 
AACTCCGGAT CAGCGTCGGC CGCAGGAGGA GGCACTCCTC ATTCTCAACA GCAGCAGGAA 
CGATCACAGG CTGGCAAGGC AAGCACACCG CCACCGATCA CCGGACGCTG GATACCACCA 
TCGTTGCGTC CACAGCATGG ACTCACCCAA AGCGAAAAGA ACGACGCCGT CTTCCGGCGT 
GTGAGGGGTA TTTTGAACAA GCTAACGCCC GAGAAGTTCC AGGAACTGAG CGATGAACTG 
TTGAAGCTGG ATCTTAACTC TATTGCTATT TTGAAAGGCG TGATTTTGCT GATATTCGAC 
AAGGCCTTAG ACGAGCCGAA GTACTCGTCT ATGTATGCGC AGCTGTGCAA GCGACTTTCC 
GAGGAAGCCC CGTCATTCGA CAAGGAACCA AGCAATTCGT CCACCTTCTT ACGCCTGCTG 
ATCGCGGTCT GCCGCGACAA GTTCAACAAC CGACTCAAGC GCGACGAGAA CGACAACAGA 
CCTCCGCCTG AAAATGAGGC CGACGAGGAG GAGCGCCGCC ATTTGGCCAA ACAGCGCATG 
CTCGGCAACG TCAAATTCAT CGGAGAGCTC AACAAGCTGG ACATGCTGTC GAAGAACGTG 
CTGCATCAGT GCATCATGGA GCTATTTGAC AAGAAGAAGA AGCGTACGGC CGGTACGCAG 
GAAATGTGCG AGGACATGGA ATGCCTGGCT CAACTGCTCA AGACTTGCGG CAAGAATCTG 
GATTCAGAAC AGGGCAAAGA GCTGATGAAC CAGTACTTCG AGAAGCTTGA ACGTCGGTCA 
AAATCATCTG AATATCCACC GCGGATACGT TTTATGCTAA AGGACGTAAT TGAATTGCGC 
CAGAACAATT GGGTGCCGCG CAAATTGGGC ACCACCGAAG GCCCCGTCCC CATAAAGCAA 
ATACGCTCGG ATGACGACTC AATCATACGC ACTCCGTTCC CCAATCGCAA TCGTGATATG 
CGCAACAATG ATCGTGACTC GGACAGCTGG ATGAATCGTT TCCATCTGAA TATCCAGCCT 
GGCGGCTATA ATGACATGTT TAGCGGACTG AGTGTTACGG GAGCTTCTCC GATTGTCTCG 
CCGTTCGCTA CGAGCAATCG TGACCGAGAC CGCGACAATC GCCAGTACAA CAATCGAGGC 
GATCGCAACA GGGATCGCGA TCAGGGCGGT AGCGGCGGAG CTAACTATGG CAATCGCTAC 
AACAAGCACA ACCAGAATGG AGGAGGCAGT GGCGGAGGAT CCTCCAACAA CCGGGACCGG 
GATGATTCGC GTAGGAATAA CGATCGTGAC AGGGATCGCA ATGACGGACA GTATGGGGGC 
AATCAGCTGC TGGGTAGTAA GGAATTGGCA CCGAGGTTCA AACGCAACTT GATCACCACG 
AACCAGGATG CGGTGGAGAA CTTGCAAATG CGCCCGGCAG CCAACTCCTT GCTCTTCCGA 
GCGGCCTCCC AGAACCAAAA GTTTCCGGCT ACGTTGCCCA TGTCAACACC GCCCAATAGC 
AGCGGCAGCA ACAACCAGGG CAGCTCGCAG GGCCAGCTCT CGAACTCCCA CTACCCTATG 
CTCGGCACGC CCACATCCCA TCTGATCAAT CCAACGCGTC CCTCGTCAAC ACCATCTGCT 
GAGTATGCTG ATAACCGAAC TCTTTTGGGA GTTCAACAGG AGAGGGGATT GAAGGCCACC 
TCTTCTTCGC CGAACTTTTC ATCAGGAGCG GAGAAGTTGG ATTTAGACGG TCAGGCGGGC 
AGAAAGGGAT CGCACGACGG CAAAGATCAG GGCAAGAATG GCTCCGTGGA GAGACCAAGC 
ACTCCGGCCG GCGGTATTGG CTCGGCCAAG CAACAAAAGA AAGATAAGGG TCCCAACAAG 
GAGGAGCTGT CTAAGAAGGC CAGTCAGTAC TTCAAGGATA GGTTCTACTA CGATGAGGAG 
GAGGATGCAG AGGTGACGGA AATTGAGATC AAGGATATTG ATGAGACAAA GACGGAGGAG 
GCCGACGCTG CTCCAGTTGA AGTCTCGGAG GTAAAGGAAG ATGCTGCAAT ACCTGTGACC 
ACTCCAGATC GCTTCACCAA GCTTGTAGAT GGTTTCCTCG AGCTTAAGCT GCCAGAGAAG 
GCGCTGAAGG ATGTGTGCAT CAATCTGCTG ATGGAGGTCT TGGACCGTGT AAACGATGTC 
TTCTTGGAGC GTGCTGTGCG CCTTCTGCAG ACATTGCGCA AGCAATCCAA TATCAAGCCG 
AACATCGTGG TCGAGATTTT CAAGCAGGTG GTCAACAAGA TGAACGAGCG AGAGGCACTC 
AATCCCCGAA TCGTTAGTCT TGTAGCCAGC TTGCTGGCAA AGACAATTTG CGAGCCAGCA 
TTGCTGAAGT TAAGCGATAT CGCCAACTTC ACGGACAATG GCCAACACTA TCCGCTGTTC 
CTGCTCGTGC TGCAGCAACT GGTCAAGGTC ATCGGCAAGG ATGCGCTGGA AGAGAAATTC 
CGGGCGAGCA AAGTTGATCT TATGAACAGC CTGCCGGAGG CGGATCGGAA CAAGGAGCGC 
TTGGCCGGGA TACTCGAGGA TCGCAAGCTT TCTTTCCTTT ATCCGCTATT GAAGGTACAG 
GCCGAGATGC TAAAACAGCT GCAAAGCGAC CCGAACCCCA ACAACTTCTA CAAGTGGATC 
AAGGCCAACG TTGACAACAA ATACTACAAG GACCCGGGCT TTATCCAAGC CCTAATGACC 
GTGGTGGTCA AGTATGTGAC CAAGGAGACG ACGCTGGCGG ACAACCTCGA CCCCAAGGAG 
CACCCTGAGA AATCGGTGAC CCAGAAGGAG CAACAGCTGC TGGAGAACTA CAGCCAGATG 
CTGCAGACCT TCCTGGGACA GAACGAGCTT CAGTTGATGG CTCTCTATGC TCTGCAAACG 
TTCTGCTACA ATGAAAATTT CCCCAAGGGC ATGCTGTGCC GTTGGTTCAA GTATCTTTAC 
GAATCTGAAA TTATAGAGGA GGAGGCATTC GTCCTGTGGA AGGAGGAGAT TTCCGACAAA 
TATCCAGGCA AAGGATCCGC TTTGTTCCAA GTGAACGCCT GGTTGACTTG GCTGCAGGAG 
GCCGAATCTG AGGACGATGA GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033943.2
CDS	475..4941
Translation	IPLSTYDAAPLLHSYDDSPQRWSGVTNGHQAGVIIDQFRGGKAIIIEGGTSTGDTKSIGGGSDLRLVITANPSEIERNYEQPTNRGGGGGGGVIGVSCGGQTPPTPSVGKTLLNTSPNLLPITTATDTFNNLKNLVKIDKGSSPSSGSNSLGGNYRKGGSNGSGYKSAGGNGGSPAISGNSEGGRNGSRSSGGPTGHFSTGYSKNSVKIRQHDSEVSPRPRRSYQGDSSSSRYYPKNSDILGQGLGGNSDSYNGSSSSGRYQQQDQQGGSHRGSRSNYHSNSDGSYNRSINSNSVGGGGSGAVGSGSGGSNYRSARNYENGNGSNGTQPRYGSGNGNGNSSNSNSSSGYNGSAASSTNSKSSYGASRGGNDGGVSSGYSSNYRDNYEDSPSHQRYNNTNSLERNGGGGSGAGASGGPRSYGRSDADRYGGTPSSRTPNLRNGSASAQISGSTSNSSNISLNSGSASAAGGGTPHSQQQQERSQAGKASTPPPITGRWIPPSLRPQHGLTQSEKNDAVFRRVRGILNKLTPEKFQELSDELLKLDLNSIAILKGVILLIFDKALDEPKYSSMYAQLCKRLSEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLKRDENDNRPPPENEADEEERRHLAKQRMLGNVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWVPRKLGTTEGPVPIKQIRSDDDSIIRTPFPNRNRDMRNNDRDSDSWMNRFHLNIQPGGYNDMFSGLSVTGASPIVSPFATSNRDRDRDNRQYNNRGDRNRDRDQGGSGGANYGNRYNKHNQNGGGSGGGSSNNRDRDDSRRNNDRDRDRNDGQYGGNQLLGSKELAPRFKRNLITTNQDAVENLQMRPAANSLLFRAASQNQKFPATLPMSTPPNSSGSNNQGSSQGQLSNSHYPMLGTPTSHLINPTRPSSTPSAEYADNRTLLGVQQERGLKATSSSPNFSSGAEKLDLDGQAGRKGSHDGKDQGKNGSVERPSTPAGGIGSAKQQKKDKGPNKEELSKKASQYFKDRFYYDEEEDAEVTEIEIKDIDETKTEEADAAPVEVSEVKEDAAIPVTTPDRFTKLVDGFLELKLPEKALKDVCINLLMEVLDRVNDVFLERAVRLLQTLRKQSNIKPNIVVEIFKQVVNKMNEREALNPRIVSLVASLLAKTICEPALLKLSDIANFTDNGQHYPLFLLVLQQLVKVIGKDALEEKFRASKVDLMNSLPEADRNKERLAGILEDRKLSFLYPLLKVQAEMLKQLQSDPNPNNFYKWIKANVDNKYYKDPGFIQALMTVVVKYVTKETTLADNLDPKEHPEKSVTQKEQQLLENYSQMLQTFLGQNELQLMALYALQTFCYNENFPKGMLCRWFKYLYESEIIEEEAFVLWKEEISDKYPGKGSALFQVNAWLTWLQEAESEDDED

Target ORF information:

RefSeq Version	NM_001038854.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NAT1, transcript variant D (NAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038854.2

ORF Insert Sequence:

ATTCCTCTAT CAACGTACGA CGCCGCTCCC CTTTTGCACT CTTACGACGA CAGTCCGCAA 
CGTTGGTCCG GAGTCACCAA CGGGCACCAG GCAGGCGTCA TCATAGATCA GTTTCGGGGC 
GGTAAAGCGA TCATCATTGA GGGAGGCACC AGCACCGGTG ATACGAAGAG CATCGGCGGC 
GGCAGCGATT TACGACTGGT AATAACGGCC AATCCCAGCG AGATCGAGCG CAATTACGAG 
CAGCCAACCA ATCGCGGTGG TGGCGGAGGC GGCGGCGTTA TCGGCGTCAG CTGCGGTGGC 
CAAACCCCAC CGACACCGTC GGTCGGCAAA ACACTGCTCA ACACCAGTCC CAATCTTCTG 
CCGATCACCA CAGCGACCGA TACGTTTAAC AACCTCAAGA ATCTGGTGAA GATCGACAAG 
GGCTCCTCGC CATCGTCAGG ATCGAATTCG CTGGGCGGCA ATTACCGCAA GGGCGGCTCT 
AACGGCTCAG GATATAAGAG CGCCGGCGGC AACGGAGGAT CTCCGGCCAT AAGCGGCAAC 
AGCGAGGGCG GCAGGAACGG CAGCCGATCA TCTGGTGGAC CGACCGGACA CTTTTCCACT 
GGCTATAGTA AGAATTCCGT CAAAATTCGC CAACACGACA GTGAGGTGAG CCCAAGACCT 
CGACGATCTT ACCAGGGTGA CTCGTCGTCG TCGCGTTACT ATCCAAAGAA TAGTGACATT 
TTGGGTCAAG GCTTAGGCGG CAACTCCGAC AGCTACAACG GCAGTTCCTC TTCTGGTAGA 
TACCAGCAGC AGGATCAGCA AGGCGGATCG CATCGTGGTA GTCGCAGCAA CTATCACTCA 
AACTCCGATG GCAGCTACAA TCGATCAATA AACTCAAACT CCGTGGGAGG AGGAGGGTCT 
GGGGCAGTCG GCTCTGGAAG CGGTGGCAGC AATTATCGCT CCGCCCGCAA CTACGAGAAC 
GGCAATGGGA GCAACGGCAC ACAGCCACGT TACGGCAGCG GCAACGGGAA CGGCAACAGC 
AGCAATTCCA ACTCTTCATC CGGTTACAAC GGATCTGCAG CAAGCTCAAC CAACAGCAAG 
TCGTCGTATG GGGCTTCGCG CGGTGGCAAC GATGGAGGCG TGAGTAGCGG ATACAGCAGC 
AACTACCGCG ACAACTACGA GGATTCACCA TCGCACCAGC GCTACAACAA CACCAACAGC 
CTAGAGCGAA ACGGAGGAGG CGGATCAGGG GCAGGAGCGA GCGGAGGACC CCGCAGCTAC 
GGCAGGTCTG ACGCAGATCG TTACGGCGGA ACCCCGTCCA GTCGCACACC CAATCTTCGC 
AACGGCAGTG CGTCTGCTCA GATCTCTGGC TCCACTTCCA ATTCTTCGAA CATTTCTCTG 
AACTCCGGAT CAGCGTCGGC CGCAGGAGGA GGCACTCCTC ATTCTCAACA GCAGCAGGAA 
CGATCACAGG CTGGCAAGGC AAGCACACCG CCACCGATCA CCGGACGCTG GATACCACCA 
TCGTTGCGTC CACAGCATGG ACTCACCCAA AGCGAAAAGA ACGACGCCGT CTTCCGGCGT 
GTGAGGGGTA TTTTGAACAA GCTAACGCCC GAGAAGTTCC AGGAACTGAG CGATGAACTG 
TTGAAGCTGG ATCTTAACTC TATTGCTATT TTGAAAGGCG TGATTTTGCT GATATTCGAC 
AAGGCCTTAG ACGAGCCGAA GTACTCGTCT ATGTATGCGC AGCTGTGCAA GCGACTTTCC 
GAGGAAGCCC CGTCATTCGA CAAGGAACCA AGCAATTCGT CCACCTTCTT ACGCCTGCTG 
ATCGCGGTCT GCCGCGACAA GTTCAACAAC CGACTCAAGC GCGACGAGAA CGACAACAGA 
CCTCCGCCTG AAAATGAGGC CGACGAGGAG GAGCGCCGCC ATTTGGCCAA ACAGCGCATG 
CTCGGCAACG TCAAATTCAT CGGAGAGCTC AACAAGCTGG ACATGCTGTC GAAGAACGTG 
CTGCATCAGT GCATCATGGA GCTATTTGAC AAGAAGAAGA AGCGTACGGC CGGTACGCAG 
GAAATGTGCG AGGACATGGA ATGCCTGGCT CAACTGCTCA AGACTTGCGG CAAGAATCTG 
GATTCAGAAC AGGGCAAAGA GCTGATGAAC CAGTACTTCG AGAAGCTTGA ACGTCGGTCA 
AAATCATCTG AATATCCACC GCGGATACGT TTTATGCTAA AGGACGTAAT TGAATTGCGC 
CAGAACAATT GGGTGCCGCG CAAATTGGGC ACCACCGAAG GCCCCGTCCC CATAAAGCAA 
ATACGCTCGG ATGACGACTC AATCATACGC ACTCCGTTCC CCAATCGCAA TCGTGATATG 
CGCAACAATG ATCGTGACTC GGACAGCTGG ATGAATCGTT TCCATCTGAA TATCCAGCCT 
GGCGGCTATA ATGACATGTT TAGCGGACTG AGTGTTACGG GAGCTTCTCC GATTGTCTCG 
CCGTTCGCTA CGAGCAATCG TGACCGAGAC CGCGACAATC GCCAGTACAA CAATCGAGGC 
GATCGCAACA GGGATCGCGA TCAGGGCGGT AGCGGCGGAG CTAACTATGG CAATCGCTAC 
AACAAGCACA ACCAGAATGG AGGAGGCAGT GGCGGAGGAT CCTCCAACAA CCGGGACCGG 
GATGATTCGC GTAGGAATAA CGATCGTGAC AGGGATCGCA ATGACGGACA GTATGGGGGC 
AATCAGCTGC TGGGTAGTAA GGAATTGGCA CCGAGGTTCA AACGCAACTT GATCACCACG 
AACCAGGATG CGGTGGAGAA CTTGCAAATG CGCCCGGCAG CCAACTCCTT GCTCTTCCGA 
GCGGCCTCCC AGAACCAAAA GTTTCCGGCT ACGTTGCCCA TGTCAACACC GCCCAATAGC 
AGCGGCAGCA ACAACCAGGG CAGCTCGCAG GGCCAGCTCT CGAACTCCCA CTACCCTATG 
CTCGGCACGC CCACATCCCA TCTGATCAAT CCAACGCGTC CCTCGTCAAC ACCATCTGCT 
GAGTATGCTG ATAACCGAAC TCTTTTGGGA GTTCAACAGG AGAGGGGATT GAAGGCCACC 
TCTTCTTCGC CGAACTTTTC ATCAGGAGCG GAGAAGTTGG ATTTAGACGG TCAGGCGGGC 
AGAAAGGGAT CGCACGACGG CAAAGATCAG GGCAAGAATG GCTCCGTGGA GAGACCAAGC 
ACTCCGGCCG GCGGTATTGG CTCGGCCAAG CAACAAAAGA AAGATAAGGG TCCCAACAAG 
GAGGAGCTGT CTAAGAAGGC CAGTCAGTAC TTCAAGGATA GGTTCTACTA CGATGAGGAG 
GAGGATGCAG AGGTGACGGA AATTGAGATC AAGGATATTG ATGAGACAAA GACGGAGGAG 
GCCGACGCTG CTCCAGTTGA AGTCTCGGAG GTAAAGGAAG ATGCTGCAAT ACCTGTGACC 
ACTCCAGATC GCTTCACCAA GCTTGTAGAT GGTTTCCTCG AGCTTAAGCT GCCAGAGAAG 
GCGCTGAAGG ATGTGTGCAT CAATCTGCTG ATGGAGGTCT TGGACCGTGT AAACGATGTC 
TTCTTGGAGC GTGCTGTGCG CCTTCTGCAG ACATTGCGCA AGCAATCCAA TATCAAGCCG 
AACATCGTGG TCGAGATTTT CAAGCAGGTG GTCAACAAGA TGAACGAGCG AGAGGCACTC 
AATCCCCGAA TCGTTAGTCT TGTAGCCAGC TTGCTGGCAA AGACAATTTG CGAGCCAGCA 
TTGCTGAAGT TAAGCGATAT CGCCAACTTC ACGGACAATG GCCAACACTA TCCGCTGTTC 
CTGCTCGTGC TGCAGCAACT GGTCAAGGTC ATCGGCAAGG ATGCGCTGGA AGAGAAATTC 
CGGGCGAGCA AAGTTGATCT TATGAACAGC CTGCCGGAGG CGGATCGGAA CAAGGAGCGC 
TTGGCCGGGA TACTCGAGGA TCGCAAGCTT TCTTTCCTTT ATCCGCTATT GAAGGTACAG 
GCCGAGATGC TAAAACAGCT GCAAAGCGAC CCGAACCCCA ACAACTTCTA CAAGTGGATC 
AAGGCCAACG TTGACAACAA ATACTACAAG GACCCGGGCT TTATCCAAGC CCTAATGACC 
GTGGTGGTCA AGTATGTGAC CAAGGAGACG ACGCTGGCGG ACAACCTCGA CCCCAAGGAG 
CACCCTGAGA AATCGGTGAC CCAGAAGGAG CAACAGCTGC TGGAGAACTA CAGCCAGATG 
CTGCAGACCT TCCTGGGACA GAACGAGCTT CAGTTGATGG CTCTCTATGC TCTGCAAACG 
TTCTGCTACA ATGAAAATTT CCCCAAGGGC ATGCTGTGCC GTTGGTTCAA GTATCTTTAC 
GAATCTGAAA TTATAGAGGA GGAGGCATTC GTCCTGTGGA AGGAGGAGAT TTCCGACAAA 
TATCCAGGCA AAGGATCCGC TTTGTTCCAA GTGAACGCCT GGTTGACTTG GCTGCAGGAG 
GCCGAATCTG AGGACGATGA GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NAT1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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