gwl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gwl
Entrez Gene ID	45969
Full Name	greatwall
Synonyms	1J,CG7719,CG7719-related,CT23435,Dmel\CG7719,EP0515,Gwl,Pk91C,Pk?2,anon-WO2004063362.73,scant
General protein information	Preferred Names greatwall Names CG7719 gene product from transcript CG7719-RA CG7719-PA CG7719-PB gwl-PA gwl-PB protein kinase-like 91C scott of the antarctic
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91C5-91C6

mRNA and Protein(s)

mRNA	Protein	Name
NM_080121.4	NP_524860.2	greatwall, isoform A
NM_001275784.1	NP_001262713.1	greatwall, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	gwl	NP_524860.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001636	XP_551171.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results indicate that the cyclic changes in Gwl localization at mitotic entry and exit are directly regulated by the antagonistic cyclin B-Cdk1 and PP2A-Tws enzymes
Title: Spatial regulation of greatwall by Cdk1 and PP2A-Tws in the cell cycle.

This mechanism explains simultaneously how PP2A-B55 and Gwl together regulate phosphorylated Endos, and how phosphorylated Endos controls PP2A-B55.
Title: Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.

Nuclear localization of Greatwall is required for its function. Spatial regulation of Gwl at mitotic entry contributes to the mitotic switch.
Title: Cell cycle regulation of Greatwall kinase nuclear localization facilitates mitotic progression.

Greatwall phosphorylates Endos at a single site, Ser68, and this is essential for Endos function
Title: Suppression of scant identifies Endos as a substrate of greatwall kinase and a negative regulator of protein phosphatase 2A in mitosis.

greatwall protein antagonizes protein phosphatase 2A function in both meiosis and mitosis
Title: PP2A-twins is antagonized by greatwall and collaborates with polo for cell cycle progression and centrosome attachment to nuclei in drosophila embryos.

The following gwl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gwl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06906	NM_080121.4 Latest version!	Drosophila melanogaster greatwall (gwl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07620	NM_001275784.1 Latest version!	Drosophila melanogaster greatwall (gwl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gwl ( NM_080121.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524860.2 Drosophila melanogaster greatwall (gwl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06906
Clone ID Related Accession (Same CDS sequence)	NM_080121.4
Accession Version	NM_080121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	greatwall, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_080121.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAACG CCGATGCGAC TTCGCAATCG GATGTCCACA TCGACTACAA AACCCCCAAG 
AAGACCCACT CGCTGATCGA CAGTGAGCAG TTGCTGGACA AGATCAACAT CCTTACCACC 
AAGCCGGAGA ATCACTCCCA GAATGCCAAG CTTCCCACTA TCAAGGATTT TGTCATCATT 
AAGCCAATCA GCCGCGGCGC CTTTGGAAAA GTCTTCCTGG GCTACAAGAA CAACGACTCG 
AAGCGGCTGT TCGCCATCAA GGTGATGCGC AAGTCAGAGA TGATCAACAA GAACATGGTG 
TCCCAGGTAA TCACGGAGCG GAATGCCCTG GCCCTCTCCC GCTCTCAGTT CTGTGTCAGT 
CTCTTCTACT CCCTGCAATC GCTCTCCTAC GTCTACCTGG TGATGGAGTA CATGGTGGGC 
GGGGATCTCA AGTCACTGCT GGCCATGTTT GGGTATTTTG ACGAGCCCAC GGCCCGGTTC 
TACGTGGCCG AGATGGTGAT GGCGCTGCAG TATCTGCATC AGCACGGAAT TGTCCACAGA 
GATATTAAAC CGGACAACAT GTTGCTCTCC TCCAGTGGCC ATGTGAAGCT TACTGATTTT 
GGGTTGAGCA AAATCGATAT GCGAAGAGAT CTGGAAATAT CAGACCTTAT AAACTGCTCA 
CCGAATTTAA ACGCCCGCAC GCCGGGCCAG CTTTTGTCGC TTACTTCGCA CCTGTCTTTT 
GGGTCAGAGA AGAAACTGAA TGACTTCGGT TCTGTTTCAA GTGGACAAAA CAACGGAATG 
GGCTCGGTGG CCACGGGGAC ATCCCACTTG CTGCAGGCCA TCAACAAGCA TAGTCTGATA 
ATGGAGCTGT CGGATAGCGA GGGCGACACC TCGCTCAATG ATGCAGAGAA GACGAGCGAT 
AGCAAGATAT CTGGTGTTTC CCCCTTCTTT TCTGCCGAGG AGGCCAATGA ATCCATTACT 
CATACCTGCA CTACAAACGT AAATCCCCAG GATAGCAGCT CGTCTTGTTC GTTCCACACT 
TGTAACTCGG CCGACCTGAG CAAGTGCTCA CCACCACTGG AGTCAAAGGA CGGTGCTGCT 
GCGGGCAATG CGATCCCCAG CAAGCGGCGC GTGGAATTCG TCTTGGATGC GGCACCCTGT 
CAGGGCTGCA AGCTGGCCGA GCAAGACAGC AGCAACATGG CCACCAATGA TGGCAAACAT 
TTGCCCAAGA TAGATAACGC AATCGAGGCT TCGTTTGAGT TTTCCATGGT GCGCAGGCGA 
TCGGTCGACG AGCGAAATCG CATCTCAAAG GGGCCGGAGG ATTCAGGGGT GTCCAGTCGC 
AAGGGCGACG ACTATTCCAG CTGCCACTTG AATCTGAATA GCGAGAGCAC GGCTTCGTCG 
ATTGAGAAGA ATGTGGACAA CCTAAGCCAA TCCAAGGAGG ATTTTAGCTG TTCAGATTAC 
TCGCGCAGTT ATAACGTGAC CAACGGCAAC GAGATGAGTG GCATCAACAT GAACTCGCCG 
TTTCGGAATC TGTCCAAGCA CTTTAAACGC CCCGACTTCC TGCGCGGCAT GAAGCGAAAG 
ATCAATTTGG TCAATCGCTC CGACAACATG TCCAGCATGG ATACCGATGG CTGCAGTTCA 
AGCAATGGAA GTACCAACAC CGGACTAACA CAGGAAATTG AAATCCTTAA TATTGGCAGC 
AGCACGCCCA AGAAGCGCAA GGCCCGTTCC TCGCCCATCC GTGGTGTGCT AAAGGTGCGC 
TCTTTATCCG ATGACGAAAT GCCCATAAAT CATTTGCTTG GTCCGGAAGC AAATGTGGCC 
AACGTGGTCT TCTCTACGCC CGTATCTTCA CAGAAGTTGC CACGTCGGGA TGGTGGTCTT 
CTTGGAAAGC TCAAGGCCAC TCGGTTCGCT CTTCCGCTGT CCATTGAGAA CAAGAAACGG 
GAGCATGCGA CCGCCGATAA GATGTCAGGC ATTCAGTACC ATCTGAAACT GTCCGACGAT 
CCCACGATGT CGCCTATCAA TCATGGAGCC GGCAATCTAC CAAAGACGCC GAAAAACGTT 
AACATCAATA CCCCGTTCCG CACACCGAAA TCAGTGCGTC GCGGGGCTCG CGTGTCTAAT 
GAGCGCATCT TGGGCACACC TGATTATCTG GCGCCGGAGT TACTGCTGAA GCAAGGTCAC 
GGTCCGGCCG TCGACTGGTG GGCTCTGGGC GTCTGTTTCT ACGAGTTCAT GACCGGCATT 
CCACCCTTTA ACGACGAGAC ACCTCAGAAA GTATTTGACA ACATTCTGAA TAAGAACATC 
GAATGGCCAG AAGGTGATGA GGCATTATCC GTCGAATCCA TGGAGGCTGT GGAACTGCTC 
CTGACCATGG ATCCCAATGA GCGACCGGCC GCAAAGGAAG TGCAACAAAT GCGTCACTTT 
GCTTGCATCG ACTGGGAGAA TATAGGCAAT ACCGAGCCCC CATTTGTCCC GACACCAGAC 
AATCCCACTG ATACGGGATA TTTCGATGCG CGCAACAATC TTCAGCACCT GCAGCTGTCC 
AATTTTGCCC TAGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524860.2
CDS	203..2743
Translation	MENADATSQSDVHIDYKTPKKTHSLIDSEQLLDKINILTTKPENHSQNAKLPTIKDFVIIKPISRGAFGKVFLGYKNNDSKRLFAIKVMRKSEMINKNMVSQVITERNALALSRSQFCVSLFYSLQSLSYVYLVMEYMVGGDLKSLLAMFGYFDEPTARFYVAEMVMALQYLHQHGIVHRDIKPDNMLLSSSGHVKLTDFGLSKIDMRRDLEISDLINCSPNLNARTPGQLLSLTSHLSFGSEKKLNDFGSVSSGQNNGMGSVATGTSHLLQAINKHSLIMELSDSEGDTSLNDAEKTSDSKISGVSPFFSAEEANESITHTCTTNVNPQDSSSSCSFHTCNSADLSKCSPPLESKDGAAAGNAIPSKRRVEFVLDAAPCQGCKLAEQDSSNMATNDGKHLPKIDNAIEASFEFSMVRRRSVDERNRISKGPEDSGVSSRKGDDYSSCHLNLNSESTASSIEKNVDNLSQSKEDFSCSDYSRSYNVTNGNEMSGINMNSPFRNLSKHFKRPDFLRGMKRKINLVNRSDNMSSMDTDGCSSSNGSTNTGLTQEIEILNIGSSTPKKRKARSSPIRGVLKVRSLSDDEMPINHLLGPEANVANVVFSTPVSSQKLPRRDGGLLGKLKATRFALPLSIENKKREHATADKMSGIQYHLKLSDDPTMSPINHGAGNLPKTPKNVNINTPFRTPKSVRRGARVSNERILGTPDYLAPELLLKQGHGPAVDWWALGVCFYEFMTGIPPFNDETPQKVFDNILNKNIEWPEGDEALSVESMEAVELLLTMDPNERPAAKEVQQMRHFACIDWENIGNTEPPFVPTPDNPTDTGYFDARNNLQHLQLSNFALEE

Target ORF information:

RefSeq Version	NM_080121.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster greatwall (gwl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080121.4

ORF Insert Sequence:

ATGGAAAACG CCGATGCGAC TTCGCAATCG GATGTCCACA TCGACTACAA AACCCCCAAG 
AAGACCCACT CGCTGATCGA CAGTGAGCAG TTGCTGGACA AGATCAACAT CCTTACCACC 
AAGCCGGAGA ATCACTCCCA GAATGCCAAG CTTCCCACTA TCAAGGATTT TGTCATCATT 
AAGCCAATCA GCCGCGGCGC CTTTGGAAAA GTCTTCCTGG GCTACAAGAA CAACGACTCG 
AAGCGGCTGT TCGCCATCAA GGTGATGCGC AAGTCAGAGA TGATCAACAA GAACATGGTG 
TCCCAGGTAA TCACGGAGCG GAATGCCCTG GCCCTCTCCC GCTCTCAGTT CTGTGTCAGT 
CTCTTCTACT CCCTGCAATC GCTCTCCTAC GTCTACCTGG TGATGGAGTA CATGGTGGGC 
GGGGATCTCA AGTCACTGCT GGCCATGTTT GGGTATTTTG ACGAGCCCAC GGCCCGGTTC 
TACGTGGCCG AGATGGTGAT GGCGCTGCAG TATCTGCATC AGCACGGAAT TGTCCACAGA 
GATATTAAAC CGGACAACAT GTTGCTCTCC TCCAGTGGCC ATGTGAAGCT TACTGATTTT 
GGGTTGAGCA AAATCGATAT GCGAAGAGAT CTGGAAATAT CAGACCTTAT AAACTGCTCA 
CCGAATTTAA ACGCCCGCAC GCCGGGCCAG CTTTTGTCGC TTACTTCGCA CCTGTCTTTT 
GGGTCAGAGA AGAAACTGAA TGACTTCGGT TCTGTTTCAA GTGGACAAAA CAACGGAATG 
GGCTCGGTGG CCACGGGGAC ATCCCACTTG CTGCAGGCCA TCAACAAGCA TAGTCTGATA 
ATGGAGCTGT CGGATAGCGA GGGCGACACC TCGCTCAATG ATGCAGAGAA GACGAGCGAT 
AGCAAGATAT CTGGTGTTTC CCCCTTCTTT TCTGCCGAGG AGGCCAATGA ATCCATTACT 
CATACCTGCA CTACAAACGT AAATCCCCAG GATAGCAGCT CGTCTTGTTC GTTCCACACT 
TGTAACTCGG CCGACCTGAG CAAGTGCTCA CCACCACTGG AGTCAAAGGA CGGTGCTGCT 
GCGGGCAATG CGATCCCCAG CAAGCGGCGC GTGGAATTCG TCTTGGATGC GGCACCCTGT 
CAGGGCTGCA AGCTGGCCGA GCAAGACAGC AGCAACATGG CCACCAATGA TGGCAAACAT 
TTGCCCAAGA TAGATAACGC AATCGAGGCT TCGTTTGAGT TTTCCATGGT GCGCAGGCGA 
TCGGTCGACG AGCGAAATCG CATCTCAAAG GGGCCGGAGG ATTCAGGGGT GTCCAGTCGC 
AAGGGCGACG ACTATTCCAG CTGCCACTTG AATCTGAATA GCGAGAGCAC GGCTTCGTCG 
ATTGAGAAGA ATGTGGACAA CCTAAGCCAA TCCAAGGAGG ATTTTAGCTG TTCAGATTAC 
TCGCGCAGTT ATAACGTGAC CAACGGCAAC GAGATGAGTG GCATCAACAT GAACTCGCCG 
TTTCGGAATC TGTCCAAGCA CTTTAAACGC CCCGACTTCC TGCGCGGCAT GAAGCGAAAG 
ATCAATTTGG TCAATCGCTC CGACAACATG TCCAGCATGG ATACCGATGG CTGCAGTTCA 
AGCAATGGAA GTACCAACAC CGGACTAACA CAGGAAATTG AAATCCTTAA TATTGGCAGC 
AGCACGCCCA AGAAGCGCAA GGCCCGTTCC TCGCCCATCC GTGGTGTGCT AAAGGTGCGC 
TCTTTATCCG ATGACGAAAT GCCCATAAAT CATTTGCTTG GTCCGGAAGC AAATGTGGCC 
AACGTGGTCT TCTCTACGCC CGTATCTTCA CAGAAGTTGC CACGTCGGGA TGGTGGTCTT 
CTTGGAAAGC TCAAGGCCAC TCGGTTCGCT CTTCCGCTGT CCATTGAGAA CAAGAAACGG 
GAGCATGCGA CCGCCGATAA GATGTCAGGC ATTCAGTACC ATCTGAAACT GTCCGACGAT 
CCCACGATGT CGCCTATCAA TCATGGAGCC GGCAATCTAC CAAAGACGCC GAAAAACGTT 
AACATCAATA CCCCGTTCCG CACACCGAAA TCAGTGCGTC GCGGGGCTCG CGTGTCTAAT 
GAGCGCATCT TGGGCACACC TGATTATCTG GCGCCGGAGT TACTGCTGAA GCAAGGTCAC 
GGTCCGGCCG TCGACTGGTG GGCTCTGGGC GTCTGTTTCT ACGAGTTCAT GACCGGCATT 
CCACCCTTTA ACGACGAGAC ACCTCAGAAA GTATTTGACA ACATTCTGAA TAAGAACATC 
GAATGGCCAG AAGGTGATGA GGCATTATCC GTCGAATCCA TGGAGGCTGT GGAACTGCTC 
CTGACCATGG ATCCCAATGA GCGACCGGCC GCAAAGGAAG TGCAACAAAT GCGTCACTTT 
GCTTGCATCG ACTGGGAGAA TATAGGCAAT ACCGAGCCCC CATTTGTCCC GACACCAGAC 
AATCCCACTG ATACGGGATA TTTCGATGCG CGCAACAATC TTCAGCACCT GCAGCTGTCC 
AATTTTGCCC TAGAAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gwl ( NM_001275784.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262713.1 Drosophila melanogaster greatwall (gwl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07620
Clone ID Related Accession (Same CDS sequence)	NM_001275784.1
Accession Version	NM_001275784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	greatwall, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAACG CCGATGCGAC TTCGCAATCG GATGTCCACA TCGACTACAA AACCCCCAAG 
AAGACCCACT CGCTGATCGA CAGTGAGCAG TTGCTGGACA AGATCAACAT CCTTACCACC 
AAGCCGGAGA ATCACTCCCA GAATGCCAAG CTTCCCACTA TCAAGGATTT TGTCATCATT 
AAGCCAATCA GCCGCGGCGC CTTTGGAAAA GTCTTCCTGG GCTACAAGAA CAACGACTCG 
AAGCGGCTGT TCGCCATCAA GGTGATGCGC AAGTCAGAGA TGATCAACAA GAACATGGTG 
TCCCAGGTAA TCACGGAGCG GAATGCCCTG GCCCTCTCCC GCTCTCAGTT CTGTGTCAGT 
CTCTTCTACT CCCTGCAATC GCTCTCCTAC GTCTACCTGG TGATGGAGTA CATGGTGGGC 
GGGGATCTCA AGTCACTGCT GGCCATGTTT GGGTATTTTG ACGAGCCCAC GGCCCGGTTC 
TACGTGGCCG AGATGGTGAT GGCGCTGCAG TATCTGCATC AGCACGGAAT TGTCCACAGA 
GATATTAAAC CGGACAACAT GTTGCTCTCC TCCAGTGGCC ATGTGAAGCT TACTGATTTT 
GGGTTGAGCA AAATCGATAT GCGAAGAGAT CTGGAAATAT CAGACCTTAT AAACTGCTCA 
CCGAATTTAA ACGCCCGCAC GCCGGGCCAG CTTTTGTCGC TTACTTCGCA CCTGTCTTTT 
GGGTCAGAGA AGAAACTGAA TGACTTCGGT TCTGTTTCAA GTGGACAAAA CAACGGAATG 
GGCTCGGTGG CCACGGGGAC ATCCCACTTG CTGCAGGCCA TCAACAAGCA TAGTCTGATA 
ATGGAGCTGT CGGATAGCGA GGGCGACACC TCGCTCAATG ATGCAGAGAA GACGAGCGAT 
AGCAAGATAT CTGGTGTTTC CCCCTTCTTT TCTGCCGAGG AGGCCAATGA ATCCATTACT 
CATACCTGCA CTACAAACGT AAATCCCCAG GATAGCAGCT CGTCTTGTTC GTTCCACACT 
TGTAACTCGG CCGACCTGAG CAAGTGCTCA CCACCACTGG AGTCAAAGGA CGGTGCTGCT 
GCGGGCAATG CGATCCCCAG CAAGCGGCGC GTGGAATTCG TCTTGGATGC GGCACCCTGT 
CAGCGAAATC GCATCTCAAA GGGGCCGGAG GATTCAGGGG TGTCCAGTCG CAAGGGCGAC 
GACTATTCCA GCTGCCACTT GAATCTGAAT AGCGAGAGCA CGGCTTCGTC GATTGAGAAG 
AATGTGGACA ACCTAAGCCA ATCCAAGGAG GATTTTAGCT GTTCAGATTA CTCGCGCAGT 
TATAACGTGA CCAACGGCAA CGAGATGAGT GGCATCAACA TGAACTCGCC GTTTCGGAAT 
CTGTCCAAGC ACTTTAAACG CCCCGACTTC CTGCGCGGCA TGAAGCGAAA GATCAATTTG 
GTCAATCGCT CCGACAACAT GTCCAGCATG GATACCGATG GCTGCAGTTC AAGCAATGGA 
AGTACCAACA CCGGACTAAC ACAGGAAATT GAAATCCTTA ATATTGGCAG CAGCACGCCC 
AAGAAGCGCA AGGCCCGTTC CTCGCCCATC CGTGGTGTGC TAAAGGTGCG CTCTTTATCC 
GATGACGAAA TGCCCATAAA TCATTTGCTT GGTCCGGAAG CAAATGTGGC CAACGTGGTC 
TTCTCTACGC CCGTATCTTC ACAGAAGTTG CCACGTCGGG ATGGTGGTCT TCTTGGAAAG 
CTCAAGGCCA CTCGGTTCGC TCTTCCGCTG TCCATTGAGA ACAAGAAACG GGAGCATGCG 
ACCGCCGATA AGATGTCAGG CATTCAGTAC CATCTGAAAC TGTCCGACGA TCCCACGATG 
TCGCCTATCA ATCATGGAGC CGGCAATCTA CCAAAGACGC CGAAAAACGT TAACATCAAT 
ACCCCGTTCC GCACACCGAA ATCAGTGCGT CGCGGGGCTC GCGTGTCTAA TGAGCGCATC 
TTGGGCACAC CTGATTATCT GGCGCCGGAG TTACTGCTGA AGCAAGGTCA CGGTCCGGCC 
GTCGACTGGT GGGCTCTGGG CGTCTGTTTC TACGAGTTCA TGACCGGCAT TCCACCCTTT 
AACGACGAGA CACCTCAGAA AGTATTTGAC AACATTCTGA ATAAGAACAT CGAATGGCCA 
GAAGGTGATG AGGCATTATC CGTCGAATCC ATGGAGGCTG TGGAACTGCT CCTGACCATG 
GATCCCAATG AGCGACCGGC CGCAAAGGAA GTGCAACAAA TGCGTCACTT TGCTTGCATC 
GACTGGGAGA ATATAGGCAA TACCGAGCCC CCATTTGTCC CGACACCAGA CAATCCCACT 
GATACGGGAT ATTTCGATGC GCGCAACAAT CTTCAGCACC TGCAGCTGTC CAATTTTGCC 
CTAGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262713.1
CDS	203..2614
Translation	MENADATSQSDVHIDYKTPKKTHSLIDSEQLLDKINILTTKPENHSQNAKLPTIKDFVIIKPISRGAFGKVFLGYKNNDSKRLFAIKVMRKSEMINKNMVSQVITERNALALSRSQFCVSLFYSLQSLSYVYLVMEYMVGGDLKSLLAMFGYFDEPTARFYVAEMVMALQYLHQHGIVHRDIKPDNMLLSSSGHVKLTDFGLSKIDMRRDLEISDLINCSPNLNARTPGQLLSLTSHLSFGSEKKLNDFGSVSSGQNNGMGSVATGTSHLLQAINKHSLIMELSDSEGDTSLNDAEKTSDSKISGVSPFFSAEEANESITHTCTTNVNPQDSSSSCSFHTCNSADLSKCSPPLESKDGAAAGNAIPSKRRVEFVLDAAPCQRNRISKGPEDSGVSSRKGDDYSSCHLNLNSESTASSIEKNVDNLSQSKEDFSCSDYSRSYNVTNGNEMSGINMNSPFRNLSKHFKRPDFLRGMKRKINLVNRSDNMSSMDTDGCSSSNGSTNTGLTQEIEILNIGSSTPKKRKARSSPIRGVLKVRSLSDDEMPINHLLGPEANVANVVFSTPVSSQKLPRRDGGLLGKLKATRFALPLSIENKKREHATADKMSGIQYHLKLSDDPTMSPINHGAGNLPKTPKNVNINTPFRTPKSVRRGARVSNERILGTPDYLAPELLLKQGHGPAVDWWALGVCFYEFMTGIPPFNDETPQKVFDNILNKNIEWPEGDEALSVESMEAVELLLTMDPNERPAAKEVQQMRHFACIDWENIGNTEPPFVPTPDNPTDTGYFDARNNLQHLQLSNFALEE

Target ORF information:

RefSeq Version	NM_001275784.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster greatwall (gwl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275784.1

ORF Insert Sequence:

ATGGAAAACG CCGATGCGAC TTCGCAATCG GATGTCCACA TCGACTACAA AACCCCCAAG 
AAGACCCACT CGCTGATCGA CAGTGAGCAG TTGCTGGACA AGATCAACAT CCTTACCACC 
AAGCCGGAGA ATCACTCCCA GAATGCCAAG CTTCCCACTA TCAAGGATTT TGTCATCATT 
AAGCCAATCA GCCGCGGCGC CTTTGGAAAA GTCTTCCTGG GCTACAAGAA CAACGACTCG 
AAGCGGCTGT TCGCCATCAA GGTGATGCGC AAGTCAGAGA TGATCAACAA GAACATGGTG 
TCCCAGGTAA TCACGGAGCG GAATGCCCTG GCCCTCTCCC GCTCTCAGTT CTGTGTCAGT 
CTCTTCTACT CCCTGCAATC GCTCTCCTAC GTCTACCTGG TGATGGAGTA CATGGTGGGC 
GGGGATCTCA AGTCACTGCT GGCCATGTTT GGGTATTTTG ACGAGCCCAC GGCCCGGTTC 
TACGTGGCCG AGATGGTGAT GGCGCTGCAG TATCTGCATC AGCACGGAAT TGTCCACAGA 
GATATTAAAC CGGACAACAT GTTGCTCTCC TCCAGTGGCC ATGTGAAGCT TACTGATTTT 
GGGTTGAGCA AAATCGATAT GCGAAGAGAT CTGGAAATAT CAGACCTTAT AAACTGCTCA 
CCGAATTTAA ACGCCCGCAC GCCGGGCCAG CTTTTGTCGC TTACTTCGCA CCTGTCTTTT 
GGGTCAGAGA AGAAACTGAA TGACTTCGGT TCTGTTTCAA GTGGACAAAA CAACGGAATG 
GGCTCGGTGG CCACGGGGAC ATCCCACTTG CTGCAGGCCA TCAACAAGCA TAGTCTGATA 
ATGGAGCTGT CGGATAGCGA GGGCGACACC TCGCTCAATG ATGCAGAGAA GACGAGCGAT 
AGCAAGATAT CTGGTGTTTC CCCCTTCTTT TCTGCCGAGG AGGCCAATGA ATCCATTACT 
CATACCTGCA CTACAAACGT AAATCCCCAG GATAGCAGCT CGTCTTGTTC GTTCCACACT 
TGTAACTCGG CCGACCTGAG CAAGTGCTCA CCACCACTGG AGTCAAAGGA CGGTGCTGCT 
GCGGGCAATG CGATCCCCAG CAAGCGGCGC GTGGAATTCG TCTTGGATGC GGCACCCTGT 
CAGCGAAATC GCATCTCAAA GGGGCCGGAG GATTCAGGGG TGTCCAGTCG CAAGGGCGAC 
GACTATTCCA GCTGCCACTT GAATCTGAAT AGCGAGAGCA CGGCTTCGTC GATTGAGAAG 
AATGTGGACA ACCTAAGCCA ATCCAAGGAG GATTTTAGCT GTTCAGATTA CTCGCGCAGT 
TATAACGTGA CCAACGGCAA CGAGATGAGT GGCATCAACA TGAACTCGCC GTTTCGGAAT 
CTGTCCAAGC ACTTTAAACG CCCCGACTTC CTGCGCGGCA TGAAGCGAAA GATCAATTTG 
GTCAATCGCT CCGACAACAT GTCCAGCATG GATACCGATG GCTGCAGTTC AAGCAATGGA 
AGTACCAACA CCGGACTAAC ACAGGAAATT GAAATCCTTA ATATTGGCAG CAGCACGCCC 
AAGAAGCGCA AGGCCCGTTC CTCGCCCATC CGTGGTGTGC TAAAGGTGCG CTCTTTATCC 
GATGACGAAA TGCCCATAAA TCATTTGCTT GGTCCGGAAG CAAATGTGGC CAACGTGGTC 
TTCTCTACGC CCGTATCTTC ACAGAAGTTG CCACGTCGGG ATGGTGGTCT TCTTGGAAAG 
CTCAAGGCCA CTCGGTTCGC TCTTCCGCTG TCCATTGAGA ACAAGAAACG GGAGCATGCG 
ACCGCCGATA AGATGTCAGG CATTCAGTAC CATCTGAAAC TGTCCGACGA TCCCACGATG 
TCGCCTATCA ATCATGGAGC CGGCAATCTA CCAAAGACGC CGAAAAACGT TAACATCAAT 
ACCCCGTTCC GCACACCGAA ATCAGTGCGT CGCGGGGCTC GCGTGTCTAA TGAGCGCATC 
TTGGGCACAC CTGATTATCT GGCGCCGGAG TTACTGCTGA AGCAAGGTCA CGGTCCGGCC 
GTCGACTGGT GGGCTCTGGG CGTCTGTTTC TACGAGTTCA TGACCGGCAT TCCACCCTTT 
AACGACGAGA CACCTCAGAA AGTATTTGAC AACATTCTGA ATAAGAACAT CGAATGGCCA 
GAAGGTGATG AGGCATTATC CGTCGAATCC ATGGAGGCTG TGGAACTGCT CCTGACCATG 
GATCCCAATG AGCGACCGGC CGCAAAGGAA GTGCAACAAA TGCGTCACTT TGCTTGCATC 
GACTGGGAGA ATATAGGCAA TACCGAGCCC CCATTTGTCC CGACACCAGA CAATCCCACT 
GATACGGGAT ATTTCGATGC GCGCAACAAT CTTCAGCACC TGCAGCTGTC CAATTTTGCC 
CTAGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gwl cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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