spir gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_165324.2	NP_724255.1	spire, isoform D
NM_001299171.1	NP_001286100.1	spire, isoform J
NM_165323.3	NP_724254.1	spire, isoform A
NM_001299170.1	NP_001286099.1	spire, isoform I
NM_001259173.1	NP_001246102.1	spire, isoform G
NM_001259174.1	NP_001246103.1	spire, isoform H
NM_001259172.2	NP_001246101.1	spire, isoform F
NM_080115.3	NP_524854.2	spire, isoform B
NM_165325.3	NP_724256.2	spire, isoform C
NM_001169549.1	NP_001163020.1	spire, isoform E

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15233	NM_165324.2 Latest version!	Drosophila melanogaster spire (spir), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15234	NM_165325.3 Latest version!	Drosophila melanogaster spire (spir), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16857	NM_001259173.1 Latest version!	Drosophila melanogaster spire (spir), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16857	NM_001299171.1 Latest version!	Drosophila melanogaster spire (spir), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16858	NM_001259174.1 Latest version!	Drosophila melanogaster spire (spir), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa22253	NM_001299170.1 Latest version!	Drosophila melanogaster spire (spir), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa15231	NM_080115.3 Latest version!	Drosophila melanogaster spire (spir), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16855	NM_001169549.1 Latest version!	Drosophila melanogaster spire (spir), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15232	NM_165323.3 Latest version!	Drosophila melanogaster spire (spir), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa16856	NM_001259172.2 Latest version!	Drosophila melanogaster spire (spir), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

spir ( NM_165324.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724255.1 Drosophila melanogaster spire (spir), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15233
Clone ID Related Accession (Same CDS sequence)	NM_165324.2
Accession Version	NM_165324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_165324.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGCAGGTGCC 
GAGAAACTGA TCAAGGCGTC GGAATCATCG GCCACCACGC AACAAGAACT GGCGAAGCTG 
GGCTTCAACG ACTGGGCACG CTTCTGGGTG CAGGTGATCG ACGAACTGCG ACGGGGAGTG 
CGGCTGAAGA AGAGCAACCA CGAGCGCACT CCCATCGAAT ATGAGCTGAC ACCCTACGAA 
ATACTCATGG GCGACATCCG AGCCAAAAAG TATCAGTTGC GCAAGGTGAT GGTCAACGGA 
GATATTCCGC CGCGCGTCAA GAAAGATGCC CACGCCATGA TCTTGGAGTT CATCAGGTCA 
CGGCCGCCGC TGAAGAAGGC CAGCGATCGC CAATTGGGCC CGCCGCGCAT GTGCGAGCCT 
TCTCCGCGGG AGCAGCTCAT GGAGTCCATA CGGAAGGGGA AGGAGCTCAA GCAGATCACC 
CCGCCAGAAG CACCGACCCT CAGGGAAAGG GTGCTTCCAA GTGCAAACTC CACGCTGTCA 
CGATCCAGAC AGCGGCTCAT CAAAGTGGAC TTCTCCAAGT TTCAGGACGA CGATCTCTTC 
TACGATGAGA ACAGCATCAG CAGCTCCCAC TCCACAGCGG CCACCCACCA GCATCATCCA 
CATTTCGCCG AGATGCACCG CTGCTCGCAG CCCAAGATGC CGCCGTATCC ATTCGGTGGC 
TACATGGTGC CCAGTCAGGC ACGACAAGAT TGCCGGGAGA CGGCGTCGCT GATGCGGCCC 
CGACGCACAA GTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724255.1
CDS	602..2359
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTSI

Target ORF information:

RefSeq Version	NM_165324.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165324.2

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGCAGGTGCC 
GAGAAACTGA TCAAGGCGTC GGAATCATCG GCCACCACGC AACAAGAACT GGCGAAGCTG 
GGCTTCAACG ACTGGGCACG CTTCTGGGTG CAGGTGATCG ACGAACTGCG ACGGGGAGTG 
CGGCTGAAGA AGAGCAACCA CGAGCGCACT CCCATCGAAT ATGAGCTGAC ACCCTACGAA 
ATACTCATGG GCGACATCCG AGCCAAAAAG TATCAGTTGC GCAAGGTGAT GGTCAACGGA 
GATATTCCGC CGCGCGTCAA GAAAGATGCC CACGCCATGA TCTTGGAGTT CATCAGGTCA 
CGGCCGCCGC TGAAGAAGGC CAGCGATCGC CAATTGGGCC CGCCGCGCAT GTGCGAGCCT 
TCTCCGCGGG AGCAGCTCAT GGAGTCCATA CGGAAGGGGA AGGAGCTCAA GCAGATCACC 
CCGCCAGAAG CACCGACCCT CAGGGAAAGG GTGCTTCCAA GTGCAAACTC CACGCTGTCA 
CGATCCAGAC AGCGGCTCAT CAAAGTGGAC TTCTCCAAGT TTCAGGACGA CGATCTCTTC 
TACGATGAGA ACAGCATCAG CAGCTCCCAC TCCACAGCGG CCACCCACCA GCATCATCCA 
CATTTCGCCG AGATGCACCG CTGCTCGCAG CCCAAGATGC CGCCGTATCC ATTCGGTGGC 
TACATGGTGC CCAGTCAGGC ACGACAAGAT TGCCGGGAGA CGGCGTCGCT GATGCGGCCC 
CGACGCACAA GTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_165325.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724256.2 Drosophila melanogaster spire (spir), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15234
Clone ID Related Accession (Same CDS sequence)	NM_165325.3
Accession Version	NM_165325.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165325.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGCCC GGCCAGCGAA ACGCTCCACA GCGGCCTCCG TCTTCCGGTC GCATCACATG 
CCGCGGGAGT CGGATCTGGT GGACATCGGC AGCGATGCCT CGCTGTACTG CGGCTCCGAC 
GGGGAGTCCA GTCAGGCCCA GTCCACATCC ACATCCACTC CGAACCCGCA AACGAGTTCC 
GACCAGGATC TGGACCAGCC TCAGCCAACA CCTCGCGCGG CACCGAGAGC ATCGGCCTCC 
AATAATCCGC CAACACCCAA GCCACGACAG GCTATCAGGT CATCAAAAGT GCTTCCAAGT 
GCAAACTCCA CGCTGTCACG ATCCAGACAG CGGCTCATCA AAGTGGACTT CTCCAAGTTT 
CAGGACGACG ATCTCTTCTA CGATGAGAAC AGCATCAGCA GCTCCCACTC CACAGCGGCC 
ACCCACCAGC ATCATCCACA TTTCGCCGAG ATGCACCGCT GCTCGCAGCC CAAGATGCCG 
CCGTATCCAT TCGGTGGCTA CATGGTGCCC AGTCAGGCAC GACAAGATTG CCGGGAGACG 
GCGTCGCTGA TGCGGCCCCG ACGCACAATG GAGCCAGCAA AGCAGGTGCC GCCTCCAGAA 
GAGCCATCCT TCACGAAGGA CGAGTACCAC AAGTTCTACG ACACGGCCCT GGAATCCTAC 
GACCTGGCCA CTCAGTGCGA GTCGAGGAGG GCTTCCTTGC GCCGCCACAC CATCGTTGGA 
TGCCAGAGCA ACCTGGACGA GACGCACTCA ATGCCGCCCA CGCGGCCCGA GTCGCGTCAA 
TCGGACGACG TCAGCAAGGA GACGCCTAAG AGAAGTCCTG CGGAGCAGAC CCATCCCTCA 
GACGAGGGCA GTTCAACATC CTCGCTTGGG CCATGGAACA AGTCCTTCAT GGACAAACAG 
ACGTGGATGG AACGGGGTGA CGATCGGCTG TCCGTAACGC TGGCGGAGAT CGTTCACATC 
CGTTCCGTCA TGACCAAGGC GGAGCTGGAG GGCCTGCCGA TGGACGTGCG CGTCAAGGAG 
GATGTTGAAA AGCGTCGCGT CTGCTTCCTG TGCCTGCGCA CTCGGTTCTC GTTCTTCGGT 
CCCTGGGGCA TTCAGTGCAA GTTGTGCCAG CGCACGGTCT GCGCCAAGTG CTATACCAAG 
ATGCGAATTC CCTCGGAGCA CTTCCGAAAC GTGCCCCTCG TGCTGATCTC GCCATCGCTG 
CTGTCCAGTC CGGCCAGCTC GAGCACCCCA TCACCCTCCC ATCATGCCCA GCAGGCGCAC 
TCATCCTCGA CGGGGAACAT CATGGACGAC CAGTTCCCCA AGTCGTTGAT AGAGCGTCTT 
TTGCGCTCCG AGTCAGATCG AAAGACTCGC AGCACTGTGG GCAGTGCCCC ATCCTCACCC 
AAGCACCAAA GATCAAACAT GAGCACGCCC GGCATTAGCG TGGGACCCGG AGCATCCTCC 
TCCTCAGCGG CCGCCACTGG ACAGGCCGTG GAGGCACTGC ACGACCAGGC GACCATGTCG 
TCCTCGTATT CCGCGGCCAT GCGACCCTCT GGCGTCCACC AGCAGCAGAA GCAGCACTAC 
AACAACGCCA TGTCGAGGAG CATGGAGGGA CCCCGAAGTC TGCCCGTGCA CAGTCCAGCA 
TATCGACCGC TCTCCAATAA CAGCACCTTG GAGAGGAAAT CCCGCTTTTC GAGGGGATTC 
AACCTGTTCT CCTCGGGCAG CCATCTTGCC CAGACGCAGG AGCAAAAGGA GAACCTGCGC 
GGCGAGCAGG TGACCGTGTG CAACGACTGC CAGGGACTGG TCAACGAGAT CACGAGCTCC 
GTGAAGCAGA AGCGCAGCTC CGCACGGAAC CGCACCATTC AGAATCTCAC CCTGGATCTG 
ACGCCCGTCT GGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724256.2
CDS	343..2220
Translation	MEARPAKRSTAASVFRSHHMPRESDLVDIGSDASLYCGSDGESSQAQSTSTSTPNPQTSSDQDLDQPQPTPRAAPRASASNNPPTPKPRQAIRSSKVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTMEPAKQVPPPEEPSFTKDEYHKFYDTALESYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK

Target ORF information:

RefSeq Version	NM_165325.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165325.3

ORF Insert Sequence:

ATGGAAGCCC GGCCAGCGAA ACGCTCCACA GCGGCCTCCG TCTTCCGGTC GCATCACATG 
CCGCGGGAGT CGGATCTGGT GGACATCGGC AGCGATGCCT CGCTGTACTG CGGCTCCGAC 
GGGGAGTCCA GTCAGGCCCA GTCCACATCC ACATCCACTC CGAACCCGCA AACGAGTTCC 
GACCAGGATC TGGACCAGCC TCAGCCAACA CCTCGCGCGG CACCGAGAGC ATCGGCCTCC 
AATAATCCGC CAACACCCAA GCCACGACAG GCTATCAGGT CATCAAAAGT GCTTCCAAGT 
GCAAACTCCA CGCTGTCACG ATCCAGACAG CGGCTCATCA AAGTGGACTT CTCCAAGTTT 
CAGGACGACG ATCTCTTCTA CGATGAGAAC AGCATCAGCA GCTCCCACTC CACAGCGGCC 
ACCCACCAGC ATCATCCACA TTTCGCCGAG ATGCACCGCT GCTCGCAGCC CAAGATGCCG 
CCGTATCCAT TCGGTGGCTA CATGGTGCCC AGTCAGGCAC GACAAGATTG CCGGGAGACG 
GCGTCGCTGA TGCGGCCCCG ACGCACAATG GAGCCAGCAA AGCAGGTGCC GCCTCCAGAA 
GAGCCATCCT TCACGAAGGA CGAGTACCAC AAGTTCTACG ACACGGCCCT GGAATCCTAC 
GACCTGGCCA CTCAGTGCGA GTCGAGGAGG GCTTCCTTGC GCCGCCACAC CATCGTTGGA 
TGCCAGAGCA ACCTGGACGA GACGCACTCA ATGCCGCCCA CGCGGCCCGA GTCGCGTCAA 
TCGGACGACG TCAGCAAGGA GACGCCTAAG AGAAGTCCTG CGGAGCAGAC CCATCCCTCA 
GACGAGGGCA GTTCAACATC CTCGCTTGGG CCATGGAACA AGTCCTTCAT GGACAAACAG 
ACGTGGATGG AACGGGGTGA CGATCGGCTG TCCGTAACGC TGGCGGAGAT CGTTCACATC 
CGTTCCGTCA TGACCAAGGC GGAGCTGGAG GGCCTGCCGA TGGACGTGCG CGTCAAGGAG 
GATGTTGAAA AGCGTCGCGT CTGCTTCCTG TGCCTGCGCA CTCGGTTCTC GTTCTTCGGT 
CCCTGGGGCA TTCAGTGCAA GTTGTGCCAG CGCACGGTCT GCGCCAAGTG CTATACCAAG 
ATGCGAATTC CCTCGGAGCA CTTCCGAAAC GTGCCCCTCG TGCTGATCTC GCCATCGCTG 
CTGTCCAGTC CGGCCAGCTC GAGCACCCCA TCACCCTCCC ATCATGCCCA GCAGGCGCAC 
TCATCCTCGA CGGGGAACAT CATGGACGAC CAGTTCCCCA AGTCGTTGAT AGAGCGTCTT 
TTGCGCTCCG AGTCAGATCG AAAGACTCGC AGCACTGTGG GCAGTGCCCC ATCCTCACCC 
AAGCACCAAA GATCAAACAT GAGCACGCCC GGCATTAGCG TGGGACCCGG AGCATCCTCC 
TCCTCAGCGG CCGCCACTGG ACAGGCCGTG GAGGCACTGC ACGACCAGGC GACCATGTCG 
TCCTCGTATT CCGCGGCCAT GCGACCCTCT GGCGTCCACC AGCAGCAGAA GCAGCACTAC 
AACAACGCCA TGTCGAGGAG CATGGAGGGA CCCCGAAGTC TGCCCGTGCA CAGTCCAGCA 
TATCGACCGC TCTCCAATAA CAGCACCTTG GAGAGGAAAT CCCGCTTTTC GAGGGGATTC 
AACCTGTTCT CCTCGGGCAG CCATCTTGCC CAGACGCAGG AGCAAAAGGA GAACCTGCGC 
GGCGAGCAGG TGACCGTGTG CAACGACTGC CAGGGACTGG TCAACGAGAT CACGAGCTCC 
GTGAAGCAGA AGCGCAGCTC CGCACGGAAC CGCACCATTC AGAATCTCAC CCTGGATCTG 
ACGCCCGTCT GGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_001299171.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286100.1 Drosophila melanogaster spire (spir), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa16857
Clone ID Related Accession (Same CDS sequence)	NM_001299171.1 , NM_001259173.1
Accession Version	NM_001299171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAAGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286100.1
CDS	602..2362
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTSI

Target ORF information:

RefSeq Version	NM_001299171.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299171.1

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAAGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_001259173.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246102.1 Drosophila melanogaster spire (spir), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa16857
Clone ID Related Accession (Same CDS sequence)	NM_001299171.1 , NM_001259173.1
Accession Version	NM_001259173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAAGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246102.1
CDS	602..2362
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTSI

Target ORF information:

RefSeq Version	NM_001259173.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259173.1

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAAGTATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_001259174.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246103.1 Drosophila melanogaster spire (spir), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa16858
Clone ID Related Accession (Same CDS sequence)	NM_001259174.1
Accession Version	NM_001259174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGGTGCCGAG 
AAACTGATCA AGGCGTCGGA ATCATCGGCC ACCACGCAAC AAGAACTGGC GAAGCTGGGC 
TTCAACGACT GGGCACGCTT CTGGGTGCAG GTGATCGACG AACTGCGACG GGGAGTGCGG 
CTGAAGAAGA GCAACCACGA GCGCACTCCC ATCGAATATG AGCTGACACC CTACGAAATA 
CTCATGGGCG ACATCCGAGC CAAAAAGTAT CAGTTGCGCA AGGTGATGGT CAACGGAGAT 
ATTCCGCCGC GCGTCAAGAA AGATGCCCAC GCCATGATCT TGGAGTTCAT CAGGTCACGG 
CCGCCGCTGA AGAAGGTGTG TAAAGCCCCC AATGCAAGTT CCCCAACGAA TTCCCAATCC 
ACTCGCATGG CCAGTACCCA AATCAACCGC AATCCCAACT GCAATCCCTC CTCATCAAAA 
TCCCCATTTC ACTTTGGCAA AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246103.1
CDS	602..2068
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKVCKAPNASSPTNSQSTRMASTQINRNPNCNPSSSKSPFHFGKV

Target ORF information:

RefSeq Version	NM_001259174.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259174.1

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGGTGCCGAG 
AAACTGATCA AGGCGTCGGA ATCATCGGCC ACCACGCAAC AAGAACTGGC GAAGCTGGGC 
TTCAACGACT GGGCACGCTT CTGGGTGCAG GTGATCGACG AACTGCGACG GGGAGTGCGG 
CTGAAGAAGA GCAACCACGA GCGCACTCCC ATCGAATATG AGCTGACACC CTACGAAATA 
CTCATGGGCG ACATCCGAGC CAAAAAGTAT CAGTTGCGCA AGGTGATGGT CAACGGAGAT 
ATTCCGCCGC GCGTCAAGAA AGATGCCCAC GCCATGATCT TGGAGTTCAT CAGGTCACGG 
CCGCCGCTGA AGAAGGTGTG TAAAGCCCCC AATGCAAGTT CCCCAACGAA TTCCCAATCC 
ACTCGCATGG CCAGTACCCA AATCAACCGC AATCCCAACT GCAATCCCTC CTCATCAAAA 
TCCCCATTTC ACTTTGGCAA AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_001299170.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286099.1 Drosophila melanogaster spire (spir), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa22253
Clone ID Related Accession (Same CDS sequence)	NM_001299170.1
Accession Version	NM_001299170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGGTGCCGAG 
AAACTGATCA AGGCGTCGGA ATCATCGGCC ACCACGCAAC AAGAACTGGC GAAGCTGGGC 
TTCAACGACT GGCACGAACT GCAGCTGTGG AGGGCACTGC AAGCACGCTT CTGGGTGCAG 
GTGATCGACG AACTGCGACG GGGAGTGCGG CTGAAGAAGA GCAACCACGA GCGCACTCCC 
ATCGAATATG AGCTGACACC CTACGAAATA CTCATGGGCG ACATCCGAGC CAAAAAGTAT 
CAGTTGCGCA AGGTGATGGT CAACGGAGAT ATTCCGCCGC GCGTCAAGAA AGATGCCCAC 
GCCATGATCT TGGAGTTCAT CAGGTCACGG CCGCCGCTGA AGAAGGTGTG TAAAGCCCCC 
AATGCAAGTT CCCCAACGAA TTCCCAATCC ACTCGCATGG CCAGTACCCA AATCAACCGC 
AATCCCAACT GCAATCCCTC CTCATCAAAA TCCCCATTTC ACTTTGGCAA AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286099.1
CDS	602..2098
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNGAEKLIKASESSATTQQELAKLGFNDWHELQLWRALQARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKVCKAPNASSPTNSQSTRMASTQINRNPNCNPSSSKSPFHFGKV

Target ORF information:

RefSeq Version	NM_001299170.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299170.1

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGGTGCCGAG 
AAACTGATCA AGGCGTCGGA ATCATCGGCC ACCACGCAAC AAGAACTGGC GAAGCTGGGC 
TTCAACGACT GGCACGAACT GCAGCTGTGG AGGGCACTGC AAGCACGCTT CTGGGTGCAG 
GTGATCGACG AACTGCGACG GGGAGTGCGG CTGAAGAAGA GCAACCACGA GCGCACTCCC 
ATCGAATATG AGCTGACACC CTACGAAATA CTCATGGGCG ACATCCGAGC CAAAAAGTAT 
CAGTTGCGCA AGGTGATGGT CAACGGAGAT ATTCCGCCGC GCGTCAAGAA AGATGCCCAC 
GCCATGATCT TGGAGTTCAT CAGGTCACGG CCGCCGCTGA AGAAGGTGTG TAAAGCCCCC 
AATGCAAGTT CCCCAACGAA TTCCCAATCC ACTCGCATGG CCAGTACCCA AATCAACCGC 
AATCCCAACT GCAATCCCTC CTCATCAAAA TCCCCATTTC ACTTTGGCAA AGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_080115.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524854.2 Drosophila melanogaster spire (spir), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15231
Clone ID Related Accession (Same CDS sequence)	NM_080115.3
Accession Version	NM_080115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_080115.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAACCTACGA CCTGGCCACT CAGTGCGAGT CGAGGAGGGC TTCCTTGCGC 
CGCCACACCA TCGTTGGATG CCAGAGCAAC CTGGACGAGA CGCACTCAAT GCCGCCCACG 
CGGCCCGAGT CGCGTCAATC GGACGACGTC AGCAAGGAGA CGCCTAAGAG AAGTCCTGCG 
GAGCAGACCC ATCCCTCAGA CGAGGGCAGT TCAACATCCT CGCTTGGGCC ATGGAACAAG 
TCCTTCATGG ACAAACAGAC GTGGATGGAA CGGGGTGACG ATCGGCTGTC CGTAACGCTG 
GCGGAGATCG TTCACATCCG TTCCGTCATG ACCAAGGCGG AGCTGGAGGG CCTGCCGATG 
GACGTGCGCG TCAAGGAGGA TGTTGAAAAG CGTCGCGTCT GCTTCCTGTG CCTGCGCACT 
CGGTTCTCGT TCTTCGGTCC CTGGGGCATT CAGTGCAAGT TGTGCCAGCG CACGGTCTGC 
GCCAAGTGCT ATACCAAGAT GCGAATTCCC TCGGAGCACT TCCGAAACGT GCCCCTCGTG 
CTGATCTCGC CATCGCTGCT GTCCAGTCCG GCCAGCTCGA GCACCCCATC ACCCTCCCAT 
CATGCCCAGC AGGCGCACTC ATCCTCGACG GGGAACATCA TGGACGACCA GTTCCCCAAG 
TCGTTGATAG AGCGTCTTTT GCGCTCCGAG TCAGATCGAA AGACTCGCAG CACTGTGGGC 
AGTGCCCCAT CCTCACCCAA GCACCAAAGA TCAAACATGA GCACGCCCGG CATTAGCGTG 
GGACCCGGAG CATCCTCCTC CTCAGCGGCC GCCACTGGAC AGGCCGTGGA GGCACTGCAC 
GACCAGGCGA CCATGTCGTC CTCGTATTCC GCGGCCATGC GACCCTCTGG CGTCCACCAG 
CAGCAGAAGC AGCACTACAA CAACGCCATG TCGAGGAGCA TGGAGGGACC CCGAAGTCTG 
CCCGTGCACA GTCCAGCATA TCGACCGCTC TCCAATAACA GCACCTTGGA GAGGAAATCC 
CGCTTTTCGA GGGGATTCAA CCTGTTCTCC TCGGGCAGCC ATCTTGCCCA GACGCAGGAG 
CAAAAGGAGA ACCTGCGCGG CGAGCAGGTG ACCGTGTGCA ACGACTGCCA GGGACTGGTC 
AACGAGATCA CGAGCTCCGT GAAGCAGAAG CGCAGCTCCG CACGGAACCG CACCATTCAG 
AATCTCACCC TGGATCTGAC GCCCGTCTGG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524854.2
CDS	602..3577
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTTYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK

Target ORF information:

RefSeq Version	NM_080115.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080115.3

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAACCTACGA CCTGGCCACT CAGTGCGAGT CGAGGAGGGC TTCCTTGCGC 
CGCCACACCA TCGTTGGATG CCAGAGCAAC CTGGACGAGA CGCACTCAAT GCCGCCCACG 
CGGCCCGAGT CGCGTCAATC GGACGACGTC AGCAAGGAGA CGCCTAAGAG AAGTCCTGCG 
GAGCAGACCC ATCCCTCAGA CGAGGGCAGT TCAACATCCT CGCTTGGGCC ATGGAACAAG 
TCCTTCATGG ACAAACAGAC GTGGATGGAA CGGGGTGACG ATCGGCTGTC CGTAACGCTG 
GCGGAGATCG TTCACATCCG TTCCGTCATG ACCAAGGCGG AGCTGGAGGG CCTGCCGATG 
GACGTGCGCG TCAAGGAGGA TGTTGAAAAG CGTCGCGTCT GCTTCCTGTG CCTGCGCACT 
CGGTTCTCGT TCTTCGGTCC CTGGGGCATT CAGTGCAAGT TGTGCCAGCG CACGGTCTGC 
GCCAAGTGCT ATACCAAGAT GCGAATTCCC TCGGAGCACT TCCGAAACGT GCCCCTCGTG 
CTGATCTCGC CATCGCTGCT GTCCAGTCCG GCCAGCTCGA GCACCCCATC ACCCTCCCAT 
CATGCCCAGC AGGCGCACTC ATCCTCGACG GGGAACATCA TGGACGACCA GTTCCCCAAG 
TCGTTGATAG AGCGTCTTTT GCGCTCCGAG TCAGATCGAA AGACTCGCAG CACTGTGGGC 
AGTGCCCCAT CCTCACCCAA GCACCAAAGA TCAAACATGA GCACGCCCGG CATTAGCGTG 
GGACCCGGAG CATCCTCCTC CTCAGCGGCC GCCACTGGAC AGGCCGTGGA GGCACTGCAC 
GACCAGGCGA CCATGTCGTC CTCGTATTCC GCGGCCATGC GACCCTCTGG CGTCCACCAG 
CAGCAGAAGC AGCACTACAA CAACGCCATG TCGAGGAGCA TGGAGGGACC CCGAAGTCTG 
CCCGTGCACA GTCCAGCATA TCGACCGCTC TCCAATAACA GCACCTTGGA GAGGAAATCC 
CGCTTTTCGA GGGGATTCAA CCTGTTCTCC TCGGGCAGCC ATCTTGCCCA GACGCAGGAG 
CAAAAGGAGA ACCTGCGCGG CGAGCAGGTG ACCGTGTGCA ACGACTGCCA GGGACTGGTC 
AACGAGATCA CGAGCTCCGT GAAGCAGAAG CGCAGCTCCG CACGGAACCG CACCATTCAG 
AATCTCACCC TGGATCTGAC GCCCGTCTGG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_001169549.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163020.1 Drosophila melanogaster spire (spir), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16855
Clone ID Related Accession (Same CDS sequence)	NM_001169549.1
Accession Version	NM_001169549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGCAGGTGCC 
GAGAAACTGA TCAAGGCGTC GGAATCATCG GCCACCACGC AACAAGAACT GGCGAAGCTG 
GGCTTCAACG ACTGGGCACG CTTCTGGGTG CAGGTGATCG ACGAACTGCG ACGGGGAGTG 
CGGCTGAAGA AGAGCAACCA CGAGCGCACT CCCATCGAAT ATGAGCTGAC ACCCTACGAA 
ATACTCATGG GCGACATCCG AGCCAAAAAG TATCAGTTGC GCAAGGTGAT GGTCAACGGA 
GATATTCCGC CGCGCGTCAA GAAAGATGCC CACGCCATGA TCTTGGAGTT CATCAGGTCA 
CGGCCGCCGC TGAAGAAGGC CAGCGATCGC CAATTGGGCC CGCCGCGCAT GTGCGAGCCT 
TCTCCGCGGG AGCAGCTCAT GGAGTCCATA CGGAAGGGGA AGGAGCTCAA GCAGATCACC 
CCGCCAGAAG CACCGACCCT CAGGGAAAGG GTGCTTCCAA GTGCAAACTC CACGCTGTCA 
CGATCCAGAC AGCGGCTCAT CAAAGTGGAC TTCTCCAAGT TTCAGGACGA CGATCTCTTC 
TACGATGAGA ACAGCATCAG CAGCTCCCAC TCCACAGCGG CCACCCACCA GCATCATCCA 
CATTTCGCCG AGATGCACCG CTGCTCGCAG CCCAAGATGC CGCCGTATCC ATTCGGTGGC 
TACATGGTGC CCAGTCAGGC ACGACAAGAT TGCCGGGAGA CGGCGTCGCT GATGCGGCCC 
CGACGCACAA CCTACGACCT GGCCACTCAG TGCGAGTCGA GGAGGGCTTC CTTGCGCCGC 
CACACCATCG TTGGATGCCA GAGCAACCTG GACGAGACGC ACTCAATGCC GCCCACGCGG 
CCCGAGTCGC GTCAATCGGA CGACGTCAGC AAGGAGACGC CTAAGAGAAG TCCTGCGGAG 
CAGACCCATC CCTCAGACGA GGGCAGTTCA ACATCCTCGC TTGGGCCATG GAACAAGTCC 
TTCATGGACA AACAGACGTG GATGGAACGG GGTGACGATC GGCTGTCCGT AACGCTGGCG 
GAGATCGTTC ACATCCGTTC CGTCATGACC AAGGCGGAGC TGGAGGGCCT GCCGATGGAC 
GTGCGCGTCA AGGAGGATGT TGAAAAGCGT CGCGTCTGCT TCCTGTGCCT GCGCACTCGG 
TTCTCGTTCT TCGGTCCCTG GGGCATTCAG TGCAAGTTGT GCCAGCGCAC GGTCTGCGCC 
AAGTGCTATA CCAAGATGCG AATTCCCTCG GAGCACTTCC GAAACGTGCC CCTCGTGCTG 
ATCTCGCCAT CGCTGCTGTC CAGTCCGGCC AGCTCGAGCA CCCCATCACC CTCCCATCAT 
GCCCAGCAGG CGCACTCATC CTCGACGGGG AACATCATGG ACGACCAGTT CCCCAAGTCG 
TTGATAGAGC GTCTTTTGCG CTCCGAGTCA GATCGAAAGA CTCGCAGCAC TGTGGGCAGT 
GCCCCATCCT CACCCAAGCA CCAAAGATCA AACATGAGCA CGCCCGGCAT TAGCGTGGGA 
CCCGGAGCAT CCTCCTCCTC AGCGGCCGCC ACTGGACAGG CCGTGGAGGC ACTGCACGAC 
CAGGCGACCA TGTCGTCCTC GTATTCCGCG GCCATGCGAC CCTCTGGCGT CCACCAGCAG 
CAGAAGCAGC ACTACAACAA CGCCATGTCG AGGAGCATGG AGGGACCCCG AAGTCTGCCC 
GTGCACAGTC CAGCATATCG ACCGCTCTCC AATAACAGCA CCTTGGAGAG GAAATCCCGC 
TTTTCGAGGG GATTCAACCT GTTCTCCTCG GGCAGCCATC TTGCCCAGAC GCAGGAGCAA 
AAGGAGAACC TGCGCGGCGA GCAGGTGACC GTGTGCAACG ACTGCCAGGG ACTGGTCAAC 
GAGATCACGA GCTCCGTGAA GCAGAAGCGC AGCTCCGCAC GGAACCGCAC CATTCAGAAT 
CTCACCCTGG ATCTGACGCC CGTCTGGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163020.1
CDS	602..3574
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTTYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK

Target ORF information:

RefSeq Version	NM_001169549.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169549.1

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGCAGGTGCC 
GAGAAACTGA TCAAGGCGTC GGAATCATCG GCCACCACGC AACAAGAACT GGCGAAGCTG 
GGCTTCAACG ACTGGGCACG CTTCTGGGTG CAGGTGATCG ACGAACTGCG ACGGGGAGTG 
CGGCTGAAGA AGAGCAACCA CGAGCGCACT CCCATCGAAT ATGAGCTGAC ACCCTACGAA 
ATACTCATGG GCGACATCCG AGCCAAAAAG TATCAGTTGC GCAAGGTGAT GGTCAACGGA 
GATATTCCGC CGCGCGTCAA GAAAGATGCC CACGCCATGA TCTTGGAGTT CATCAGGTCA 
CGGCCGCCGC TGAAGAAGGC CAGCGATCGC CAATTGGGCC CGCCGCGCAT GTGCGAGCCT 
TCTCCGCGGG AGCAGCTCAT GGAGTCCATA CGGAAGGGGA AGGAGCTCAA GCAGATCACC 
CCGCCAGAAG CACCGACCCT CAGGGAAAGG GTGCTTCCAA GTGCAAACTC CACGCTGTCA 
CGATCCAGAC AGCGGCTCAT CAAAGTGGAC TTCTCCAAGT TTCAGGACGA CGATCTCTTC 
TACGATGAGA ACAGCATCAG CAGCTCCCAC TCCACAGCGG CCACCCACCA GCATCATCCA 
CATTTCGCCG AGATGCACCG CTGCTCGCAG CCCAAGATGC CGCCGTATCC ATTCGGTGGC 
TACATGGTGC CCAGTCAGGC ACGACAAGAT TGCCGGGAGA CGGCGTCGCT GATGCGGCCC 
CGACGCACAA CCTACGACCT GGCCACTCAG TGCGAGTCGA GGAGGGCTTC CTTGCGCCGC 
CACACCATCG TTGGATGCCA GAGCAACCTG GACGAGACGC ACTCAATGCC GCCCACGCGG 
CCCGAGTCGC GTCAATCGGA CGACGTCAGC AAGGAGACGC CTAAGAGAAG TCCTGCGGAG 
CAGACCCATC CCTCAGACGA GGGCAGTTCA ACATCCTCGC TTGGGCCATG GAACAAGTCC 
TTCATGGACA AACAGACGTG GATGGAACGG GGTGACGATC GGCTGTCCGT AACGCTGGCG 
GAGATCGTTC ACATCCGTTC CGTCATGACC AAGGCGGAGC TGGAGGGCCT GCCGATGGAC 
GTGCGCGTCA AGGAGGATGT TGAAAAGCGT CGCGTCTGCT TCCTGTGCCT GCGCACTCGG 
TTCTCGTTCT TCGGTCCCTG GGGCATTCAG TGCAAGTTGT GCCAGCGCAC GGTCTGCGCC 
AAGTGCTATA CCAAGATGCG AATTCCCTCG GAGCACTTCC GAAACGTGCC CCTCGTGCTG 
ATCTCGCCAT CGCTGCTGTC CAGTCCGGCC AGCTCGAGCA CCCCATCACC CTCCCATCAT 
GCCCAGCAGG CGCACTCATC CTCGACGGGG AACATCATGG ACGACCAGTT CCCCAAGTCG 
TTGATAGAGC GTCTTTTGCG CTCCGAGTCA GATCGAAAGA CTCGCAGCAC TGTGGGCAGT 
GCCCCATCCT CACCCAAGCA CCAAAGATCA AACATGAGCA CGCCCGGCAT TAGCGTGGGA 
CCCGGAGCAT CCTCCTCCTC AGCGGCCGCC ACTGGACAGG CCGTGGAGGC ACTGCACGAC 
CAGGCGACCA TGTCGTCCTC GTATTCCGCG GCCATGCGAC CCTCTGGCGT CCACCAGCAG 
CAGAAGCAGC ACTACAACAA CGCCATGTCG AGGAGCATGG AGGGACCCCG AAGTCTGCCC 
GTGCACAGTC CAGCATATCG ACCGCTCTCC AATAACAGCA CCTTGGAGAG GAAATCCCGC 
TTTTCGAGGG GATTCAACCT GTTCTCCTCG GGCAGCCATC TTGCCCAGAC GCAGGAGCAA 
AAGGAGAACC TGCGCGGCGA GCAGGTGACC GTGTGCAACG ACTGCCAGGG ACTGGTCAAC 
GAGATCACGA GCTCCGTGAA GCAGAAGCGC AGCTCCGCAC GGAACCGCAC CATTCAGAAT 
CTCACCCTGG ATCTGACGCC CGTCTGGAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_165323.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724254.1 Drosophila melanogaster spire (spir), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15232
Clone ID Related Accession (Same CDS sequence)	NM_165323.3
Accession Version	NM_165323.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165323.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAATGGAGCC AGCAAAGCAG GTGCCGCCTC CAGAAGAGCC ATCCTTCACG 
AAGGACGAGT ACCACAAGTT CTACGACACG GCCCTGGAAT CCTACGACCT GGCCACTCAG 
TGCGAGTCGA GGAGGGCTTC CTTGCGCCGC CACACCATCG TTGGATGCCA GAGCAACCTG 
GACGAGACGC ACTCAATGCC GCCCACGCGG CCCGAGTCGC GTCAATCGGA CGACGTCAGC 
AAGGAGACGC CTAAGAGAAG TCCTGCGGAG CAGACCCATC CCTCAGACGA GGGCAGTTCA 
ACATCCTCGC TTGGGCCATG GAACAAGTCC TTCATGGACA AACAGACGTG GATGGAACGG 
GGTGACGATC GGCTGTCCGT AACGCTGGCG GAGATCGTTC ACATCCGTTC CGTCATGACC 
AAGGCGGAGC TGGAGGGCCT GCCGATGGAC GTGCGCGTCA AGGAGGATGT TGAAAAGCGT 
CGCGTCTGCT TCCTGTGCCT GCGCACTCGG TTCTCGTTCT TCGGTCCCTG GGGCATTCAG 
TGCAAGTTGT GCCAGCGCAC GGTCTGCGCC AAGTGCTATA CCAAGATGCG AATTCCCTCG 
GAGCACTTCC GAAACGTGCC CCTCGTGCTG ATCTCGCCAT CGCTGCTGTC CAGTCCGGCC 
AGCTCGAGCA CCCCATCACC CTCCCATCAT GCCCAGCAGG CGCACTCATC CTCGACGGGG 
AACATCATGG ACGACCAGTT CCCCAAGTCG TTGATAGAGC GTCTTTTGCG CTCCGAGTCA 
GATCGAAAGA CTCGCAGCAC TGTGGGCAGT GCCCCATCCT CACCCAAGCA CCAAAGATCA 
AACATGAGCA CGCCCGGCAT TAGCGTGGGA CCCGGAGCAT CCTCCTCCTC AGCGGCCGCC 
ACTGGACAGG CCGTGGAGGC ACTGCACGAC CAGGCGACCA TGTCGTCCTC GTATTCCGCG 
GCCATGCGAC CCTCTGGCGT CCACCAGCAG CAGAAGCAGC ACTACAACAA CGCCATGTCG 
AGGAGCATGG AGGGACCCCG AAGTCTGCCC GTGCACAGTC CAGCATATCG ACCGCTCTCC 
AATAACAGCA CCTTGGAGAG GAAATCCCGC TTTTCGAGGG GATTCAACCT GTTCTCCTCG 
GGCAGCCATC TTGCCCAGAC GCAGGAGCAA AAGGAGAACC TGCGCGGCGA GCAGGTGACC 
GTGTGCAACG ACTGCCAGGG ACTGGTCAAC GAGATCACGA GCTCCGTGAA GCAGAAGCGC 
AGCTCCGCAC GGAACCGCAC CATTCAGAAT CTCACCCTGG ATCTGACGCC CGTCTGGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724254.1
CDS	602..3664
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTMEPAKQVPPPEEPSFTKDEYHKFYDTALESYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK

Target ORF information:

RefSeq Version	NM_165323.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165323.3

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGAAGCAGGT 
GCCGAGAAAC TGATCAAGGC GTCGGAATCA TCGGCCACCA CGCAACAAGA ACTGGCGAAG 
CTGGGCTTCA ACGACTGGGC ACGCTTCTGG GTGCAGGTGA TCGACGAACT GCGACGGGGA 
GTGCGGCTGA AGAAGAGCAA CCACGAGCGC ACTCCCATCG AATATGAGCT GACACCCTAC 
GAAATACTCA TGGGCGACAT CCGAGCCAAA AAGTATCAGT TGCGCAAGGT GATGGTCAAC 
GGAGATATTC CGCCGCGCGT CAAGAAAGAT GCCCACGCCA TGATCTTGGA GTTCATCAGG 
TCACGGCCGC CGCTGAAGAA GGCCAGCGAT CGCCAATTGG GCCCGCCGCG CATGTGCGAG 
CCTTCTCCGC GGGAGCAGCT CATGGAGTCC ATACGGAAGG GGAAGGAGCT CAAGCAGATC 
ACCCCGCCAG AAGCACCGAC CCTCAGGGAA AGGGTGCTTC CAAGTGCAAA CTCCACGCTG 
TCACGATCCA GACAGCGGCT CATCAAAGTG GACTTCTCCA AGTTTCAGGA CGACGATCTC 
TTCTACGATG AGAACAGCAT CAGCAGCTCC CACTCCACAG CGGCCACCCA CCAGCATCAT 
CCACATTTCG CCGAGATGCA CCGCTGCTCG CAGCCCAAGA TGCCGCCGTA TCCATTCGGT 
GGCTACATGG TGCCCAGTCA GGCACGACAA GATTGCCGGG AGACGGCGTC GCTGATGCGG 
CCCCGACGCA CAATGGAGCC AGCAAAGCAG GTGCCGCCTC CAGAAGAGCC ATCCTTCACG 
AAGGACGAGT ACCACAAGTT CTACGACACG GCCCTGGAAT CCTACGACCT GGCCACTCAG 
TGCGAGTCGA GGAGGGCTTC CTTGCGCCGC CACACCATCG TTGGATGCCA GAGCAACCTG 
GACGAGACGC ACTCAATGCC GCCCACGCGG CCCGAGTCGC GTCAATCGGA CGACGTCAGC 
AAGGAGACGC CTAAGAGAAG TCCTGCGGAG CAGACCCATC CCTCAGACGA GGGCAGTTCA 
ACATCCTCGC TTGGGCCATG GAACAAGTCC TTCATGGACA AACAGACGTG GATGGAACGG 
GGTGACGATC GGCTGTCCGT AACGCTGGCG GAGATCGTTC ACATCCGTTC CGTCATGACC 
AAGGCGGAGC TGGAGGGCCT GCCGATGGAC GTGCGCGTCA AGGAGGATGT TGAAAAGCGT 
CGCGTCTGCT TCCTGTGCCT GCGCACTCGG TTCTCGTTCT TCGGTCCCTG GGGCATTCAG 
TGCAAGTTGT GCCAGCGCAC GGTCTGCGCC AAGTGCTATA CCAAGATGCG AATTCCCTCG 
GAGCACTTCC GAAACGTGCC CCTCGTGCTG ATCTCGCCAT CGCTGCTGTC CAGTCCGGCC 
AGCTCGAGCA CCCCATCACC CTCCCATCAT GCCCAGCAGG CGCACTCATC CTCGACGGGG 
AACATCATGG ACGACCAGTT CCCCAAGTCG TTGATAGAGC GTCTTTTGCG CTCCGAGTCA 
GATCGAAAGA CTCGCAGCAC TGTGGGCAGT GCCCCATCCT CACCCAAGCA CCAAAGATCA 
AACATGAGCA CGCCCGGCAT TAGCGTGGGA CCCGGAGCAT CCTCCTCCTC AGCGGCCGCC 
ACTGGACAGG CCGTGGAGGC ACTGCACGAC CAGGCGACCA TGTCGTCCTC GTATTCCGCG 
GCCATGCGAC CCTCTGGCGT CCACCAGCAG CAGAAGCAGC ACTACAACAA CGCCATGTCG 
AGGAGCATGG AGGGACCCCG AAGTCTGCCC GTGCACAGTC CAGCATATCG ACCGCTCTCC 
AATAACAGCA CCTTGGAGAG GAAATCCCGC TTTTCGAGGG GATTCAACCT GTTCTCCTCG 
GGCAGCCATC TTGCCCAGAC GCAGGAGCAA AAGGAGAACC TGCGCGGCGA GCAGGTGACC 
GTGTGCAACG ACTGCCAGGG ACTGGTCAAC GAGATCACGA GCTCCGTGAA GCAGAAGCGC 
AGCTCCGCAC GGAACCGCAC CATTCAGAAT CTCACCCTGG ATCTGACGCC CGTCTGGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spir ( NM_001259172.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246101.1 Drosophila melanogaster spire (spir), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16856
Clone ID Related Accession (Same CDS sequence)	NM_001259172.2
Accession Version	NM_001259172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spire, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259172.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGGTGCCGAG 
AAACTGATCA AGGCGTCGGA ATCATCGGCC ACCACGCAAC AAGAACTGGC GAAGCTGGGC 
TTCAACGACT GGGCACGCTT CTGGGTGCAG GTGATCGACG AACTGCGACG GGGAGTGCGG 
CTGAAGAAGA GCAACCACGA GCGCACTCCC ATCGAATATG AGCTGACACC CTACGAAATA 
CTCATGGGCG ACATCCGAGC CAAAAAGTAT CAGTTGCGCA AGGTGATGGT CAACGGAGAT 
ATTCCGCCGC GCGTCAAGAA AGATGCCCAC GCCATGATCT TGGAGTTCAT CAGGTCACGG 
CCGCCGCTGA AGAAGGCCAG CGATCGCCAA TTGGGCCCGC CGCGCATGTG CGAGCCTTCT 
CCGCGGGAGC AGCTCATGGA GTCCATACGG AAGGGGAAGG AGCTCAAGCA GATCACCCCG 
CCAGAAGCAC CGACCCTCAG GGAAAGGGTG CTTCCAAGTG CAAACTCCAC GCTGTCACGA 
TCCAGACAGC GGCTCATCAA AGTGGACTTC TCCAAGTTTC AGGACGACGA TCTCTTCTAC 
GATGAGAACA GCATCAGCAG CTCCCACTCC ACAGCGGCCA CCCACCAGCA TCATCCACAT 
TTCGCCGAGA TGCACCGCTG CTCGCAGCCC AAGATGCCGC CGTATCCATT CGGTGGCTAC 
ATGGTGCCCA GTCAGGCACG ACAAGATTGC CGGGAGACGG CGTCGCTGAT GCGGCCCCGA 
CGCACAATGG AGCCAGCAAA GCAGGTGCCG CCTCCAGAAG AGCCATCCTT CACGAAGGAC 
GAGTACCACA AGTTCTACGA CACGGCCCTG GAATCCTACG ACCTGGCCAC TCAGTGCGAG 
TCGAGGAGGG CTTCCTTGCG CCGCCACACC ATCGTTGGAT GCCAGAGCAA CCTGGACGAG 
ACGCACTCAA TGCCGCCCAC GCGGCCCGAG TCGCGTCAAT CGGACGACGT CAGCAAGGAG 
ACGCCTAAGA GAAGTCCTGC GGAGCAGACC CATCCCTCAG ACGAGGGCAG TTCAACATCC 
TCGCTTGGGC CATGGAACAA GTCCTTCATG GACAAACAGA CGTGGATGGA ACGGGGTGAC 
GATCGGCTGT CCGTAACGCT GGCGGAGATC GTTCACATCC GTTCCGTCAT GACCAAGGCG 
GAGCTGGAGG GCCTGCCGAT GGACGTGCGC GTCAAGGAGG ATGTTGAAAA GCGTCGCGTC 
TGCTTCCTGT GCCTGCGCAC TCGGTTCTCG TTCTTCGGTC CCTGGGGCAT TCAGTGCAAG 
TTGTGCCAGC GCACGGTCTG CGCCAAGTGC TATACCAAGA TGCGAATTCC CTCGGAGCAC 
TTCCGAAACG TGCCCCTCGT GCTGATCTCG CCATCGCTGC TGTCCAGTCC GGCCAGCTCG 
AGCACCCCAT CACCCTCCCA TCATGCCCAG CAGGCGCACT CATCCTCGAC GGGGAACATC 
ATGGACGACC AGTTCCCCAA GTCGTTGATA GAGCGTCTTT TGCGCTCCGA GTCAGATCGA 
AAGACTCGCA GCACTGTGGG CAGTGCCCCA TCCTCACCCA AGCACCAAAG ATCAAACATG 
AGCACGCCCG GCATTAGCGT GGGACCCGGA GCATCCTCCT CCTCAGCGGC CGCCACTGGA 
CAGGCCGTGG AGGCACTGCA CGACCAGGCG ACCATGTCGT CCTCGTATTC CGCGGCCATG 
CGACCCTCTG GCGTCCACCA GCAGCAGAAG CAGCACTACA ACAACGCCAT GTCGAGGAGC 
ATGGAGGGAC CCCGAAGTCT GCCCGTGCAC AGTCCAGCAT ATCGACCGCT CTCCAATAAC 
AGCACCTTGG AGAGGAAATC CCGCTTTTCG AGGGGATTCA ACCTGTTCTC CTCGGGCAGC 
CATCTTGCCC AGACGCAGGA GCAAAAGGAG AACCTGCGCG GCGAGCAGGT GACCGTGTGC 
AACGACTGCC AGGGACTGGT CAACGAGATC ACGAGCTCCG TGAAGCAGAA GCGCAGCTCC 
GCACGGAACC GCACCATTCA GAATCTCACC CTGGATCTGA CGCCCGTCTG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246101.1
CDS	602..3658
Translation	MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAMSSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYHQVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIVDQPDHSASSSGDSSVINRAFDNSNHHHHHQHHHPPLVVSHRKIISELAEIVYTALDYNLPEDEECQVSQELENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIETCRNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNGAEKLIKASESSATTQQELAKLGFNDWARFWVQVIDELRRGVRLKKSNHERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKASDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPHFAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTMEPAKQVPPPEEPSFTKDEYHKFYDTALESYDLATQCESRRASLRRHTIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSFMDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRFSFFGPWGIQCKLCQRTVCAKCYTKMRIPSEHFRNVPLVLISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEALHDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERKSRFSRGFNLFSSGSHLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK

Target ORF information:

RefSeq Version	NM_001259172.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spire (spir), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259172.2

ORF Insert Sequence:

ATGACGGAGC ACCAGGCCGA GGAGCAGGCG GACACGCCGC CCACCAAGGT CAAGGCCACG 
CCCACACCCA CCCCGAGTGG AAAGTTCAAG GACGCGAAGG AAGACGCCTT CCTGTCCACA 
TCGCCGGACA GCGCCAACGG CGATGCCCAA CACAAGCTGC CAGCGGACCA GCTGGCCATG 
TCCTCCAGTG CGCATCCCCA GCAGGCGGGC CAAGCCCGCC CGCTCATCCT GCAAGCCTTC 
CACCGCTGCT CCTCGCCAGA ACAGTGCGTC ACGCTGCACG ACATCCTGGA CTCCTTTAAG 
GCGCCGCTCT CCGAGGACCA GGCCTGGGCC CTGATCCACC AGTTCGCCGG GCTCTACCAC 
CAGGTGGCCG TACAAGCGCA CACCTGTGCC GCGGACTACG AGGCCGCCCT GCCCACCGGA 
TTCGAGCTGC ACTTCCATCG CGACGGCAGC GTCCACTTCT CCGGCCCGGA TCAGCTGACG 
CCTAAGGAGC AACTGCAGCA GGAGCAGATC CCCCTCCCCC CGCAGCACGA CGTGATTGTG 
GATCAGCCGG ACCACAGCGC CAGCAGCAGC GGCGACAGCA GCGTCATCAA CAGAGCGTTC 
GACAACAGCA ATCACCACCA CCACCATCAA CATCATCACC CGCCGCTCGT CGTCTCGCAT 
AGAAAGATTA TCAGCGAACT GGCGGAAATC GTCTACACCG CCTTGGATTA CAATCTGCCG 
GAGGACGAGG AGTGCCAAGT GTCACAGGAG CTGGAGAATC TGTTCAACTT TATGACGGCA 
GATGAGACAG ACGACGATTG CATTGACGAG GGCATCGACG AGGGCGACAA GCGCTGGGAC 
GACGAATCCG AGGAGGAGCG CAACGATACC AAAGAACTAG AGCACATAAT CGAGACCTGC 
AGAAATCACA TAAAAACCAC CCTGCCTGAG AACCACTATA GAGCTGTCTG CAGAGCCCTC 
GTGACGGAGA CCATAGAGCT CCGAGTATTT CTGCAGCAGG TCCTGAACAA TGGTGCCGAG 
AAACTGATCA AGGCGTCGGA ATCATCGGCC ACCACGCAAC AAGAACTGGC GAAGCTGGGC 
TTCAACGACT GGGCACGCTT CTGGGTGCAG GTGATCGACG AACTGCGACG GGGAGTGCGG 
CTGAAGAAGA GCAACCACGA GCGCACTCCC ATCGAATATG AGCTGACACC CTACGAAATA 
CTCATGGGCG ACATCCGAGC CAAAAAGTAT CAGTTGCGCA AGGTGATGGT CAACGGAGAT 
ATTCCGCCGC GCGTCAAGAA AGATGCCCAC GCCATGATCT TGGAGTTCAT CAGGTCACGG 
CCGCCGCTGA AGAAGGCCAG CGATCGCCAA TTGGGCCCGC CGCGCATGTG CGAGCCTTCT 
CCGCGGGAGC AGCTCATGGA GTCCATACGG AAGGGGAAGG AGCTCAAGCA GATCACCCCG 
CCAGAAGCAC CGACCCTCAG GGAAAGGGTG CTTCCAAGTG CAAACTCCAC GCTGTCACGA 
TCCAGACAGC GGCTCATCAA AGTGGACTTC TCCAAGTTTC AGGACGACGA TCTCTTCTAC 
GATGAGAACA GCATCAGCAG CTCCCACTCC ACAGCGGCCA CCCACCAGCA TCATCCACAT 
TTCGCCGAGA TGCACCGCTG CTCGCAGCCC AAGATGCCGC CGTATCCATT CGGTGGCTAC 
ATGGTGCCCA GTCAGGCACG ACAAGATTGC CGGGAGACGG CGTCGCTGAT GCGGCCCCGA 
CGCACAATGG AGCCAGCAAA GCAGGTGCCG CCTCCAGAAG AGCCATCCTT CACGAAGGAC 
GAGTACCACA AGTTCTACGA CACGGCCCTG GAATCCTACG ACCTGGCCAC TCAGTGCGAG 
TCGAGGAGGG CTTCCTTGCG CCGCCACACC ATCGTTGGAT GCCAGAGCAA CCTGGACGAG 
ACGCACTCAA TGCCGCCCAC GCGGCCCGAG TCGCGTCAAT CGGACGACGT CAGCAAGGAG 
ACGCCTAAGA GAAGTCCTGC GGAGCAGACC CATCCCTCAG ACGAGGGCAG TTCAACATCC 
TCGCTTGGGC CATGGAACAA GTCCTTCATG GACAAACAGA CGTGGATGGA ACGGGGTGAC 
GATCGGCTGT CCGTAACGCT GGCGGAGATC GTTCACATCC GTTCCGTCAT GACCAAGGCG 
GAGCTGGAGG GCCTGCCGAT GGACGTGCGC GTCAAGGAGG ATGTTGAAAA GCGTCGCGTC 
TGCTTCCTGT GCCTGCGCAC TCGGTTCTCG TTCTTCGGTC CCTGGGGCAT TCAGTGCAAG 
TTGTGCCAGC GCACGGTCTG CGCCAAGTGC TATACCAAGA TGCGAATTCC CTCGGAGCAC 
TTCCGAAACG TGCCCCTCGT GCTGATCTCG CCATCGCTGC TGTCCAGTCC GGCCAGCTCG 
AGCACCCCAT CACCCTCCCA TCATGCCCAG CAGGCGCACT CATCCTCGAC GGGGAACATC 
ATGGACGACC AGTTCCCCAA GTCGTTGATA GAGCGTCTTT TGCGCTCCGA GTCAGATCGA 
AAGACTCGCA GCACTGTGGG CAGTGCCCCA TCCTCACCCA AGCACCAAAG ATCAAACATG 
AGCACGCCCG GCATTAGCGT GGGACCCGGA GCATCCTCCT CCTCAGCGGC CGCCACTGGA 
CAGGCCGTGG AGGCACTGCA CGACCAGGCG ACCATGTCGT CCTCGTATTC CGCGGCCATG 
CGACCCTCTG GCGTCCACCA GCAGCAGAAG CAGCACTACA ACAACGCCAT GTCGAGGAGC 
ATGGAGGGAC CCCGAAGTCT GCCCGTGCAC AGTCCAGCAT ATCGACCGCT CTCCAATAAC 
AGCACCTTGG AGAGGAAATC CCGCTTTTCG AGGGGATTCA ACCTGTTCTC CTCGGGCAGC 
CATCTTGCCC AGACGCAGGA GCAAAAGGAG AACCTGCGCG GCGAGCAGGT GACCGTGTGC 
AACGACTGCC AGGGACTGGT CAACGAGATC ACGAGCTCCG TGAAGCAGAA GCGCAGCTCC 
GCACGGAACC GCACCATTCA GAATCTCACC CTGGATCTGA CGCCCGTCTG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	spir
Entrez Gene ID	45931
Full Name	spire
Synonyms	38C.34,38C.37,CG10076,CG18621,Dmel\CG10076,NP2788,Spir,Spire,l(2)08327,p150-Spir,p150Spir
General protein information	Preferred Names spire Names CG10076 gene product from transcript CG10076-RJ CG10076-PA CG10076-PB CG10076-PC CG10076-PD CG10076-PE CG10076-PF CG10076-PG CG10076-PH CG10076-PI CG10076-PJ spir-PA spir-PB spir-PC spir-PD spir-PE spir-PF spir-PG spir-PH spir-PI spir-PJ
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38C5-38C5\|2-54 cM

Drosophila melanogaster (fruit fly)	spir	NP_001246101.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008100	XP_317356.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

spir cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

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