shi cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following shi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa02395 NM_001169295.1
Latest version!
Drosophila melanogaster shibire (shi), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa02395 NM_206742.1
Latest version!
Drosophila melanogaster shibire (shi), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa02395 NM_206743.2
Latest version!
Drosophila melanogaster shibire (shi), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa02395 NM_167470.3
Latest version!
Drosophila melanogaster shibire (shi), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa02396 NM_206745.3
Latest version!
Drosophila melanogaster shibire (shi), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa02396 NM_206744.3
Latest version!
Drosophila melanogaster shibire (shi), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa02396 NM_001042813.2
Latest version!
Drosophila melanogaster shibire (shi), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa02396 NM_167471.5
Latest version!
Drosophila melanogaster shibire (shi), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa02396 NM_001042814.2
Latest version!
Drosophila melanogaster shibire (shi), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa02396 NM_080114.4
Latest version!
Drosophila melanogaster shibire (shi), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OFa02744 NM_001272659.2
Latest version!
Drosophila melanogaster shibire (shi), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa02745 NM_001272660.2
Latest version!
Drosophila melanogaster shibire (shi), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa02746 NM_001169297.2
Latest version!
Drosophila melanogaster shibire (shi), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa02748 NM_001169296.1
Latest version!
Drosophila melanogaster shibire (shi), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa22252 NM_001298377.1
Latest version!
Drosophila melanogaster shibire (shi), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa02395
    Clone ID Related Accession (Same CDS sequence) NM_001169295.1 , NM_206743.2 , NM_206742.1 , NM_167470.3
    Accession Version NM_001169295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162766.1
    CDS419..3052
    Translation

    Target ORF information:

    RefSeq Version NM_001169295.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02395
    Clone ID Related Accession (Same CDS sequence) NM_001169295.1 , NM_206743.2 , NM_206742.1 , NM_167470.3
    Accession Version NM_206743.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206743.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996466.1
    CDS230..2863
    Translation

    Target ORF information:

    RefSeq Version NM_206743.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206743.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02395
    Clone ID Related Accession (Same CDS sequence) NM_001169295.1 , NM_206743.2 , NM_206742.1 , NM_167470.3
    Accession Version NM_206742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996465.1
    CDS229..2862
    Translation

    Target ORF information:

    RefSeq Version NM_206742.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02395
    Clone ID Related Accession (Same CDS sequence) NM_001169295.1 , NM_206743.2 , NM_206742.1 , NM_167470.3
    Accession Version NM_167470.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167470.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_727910.1
    CDS339..2972
    Translation

    Target ORF information:

    RefSeq Version NM_167470.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_167470.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCCCGTGTG TCCGGAGCTG TCGGCGGCGC CATTGTCCAG
    CAATCGGGAG CCAATCGCTA TGTGCCGGAG AGTATGCGTG GACAGGTGAA CCAGGCGGTG
    GGCCAGGCAG CCATCAATGA GTTGTCCAAT GCCTTCAGTT CGCGATTCAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02396
    Clone ID Related Accession (Same CDS sequence) NM_001042814.2 , NM_080114.4 , NM_167471.5 , NM_206744.3 , NM_001042813.2 , NM_206745.3
    Accession Version NM_001042814.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001042814.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036279.1
    CDS560..3052
    Translation

    Target ORF information:

    RefSeq Version NM_001042814.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042814.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02396
    Clone ID Related Accession (Same CDS sequence) NM_001042814.2 , NM_080114.4 , NM_167471.5 , NM_206744.3 , NM_001042813.2 , NM_206745.3
    Accession Version NM_080114.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080114.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524853.2
    CDS117..2609
    Translation

    Target ORF information:

    RefSeq Version NM_080114.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_080114.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02396
    Clone ID Related Accession (Same CDS sequence) NM_001042814.2 , NM_080114.4 , NM_167471.5 , NM_206744.3 , NM_001042813.2 , NM_206745.3
    Accession Version NM_167471.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167471.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_727911.1
    CDS419..2911
    Translation

    Target ORF information:

    RefSeq Version NM_167471.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_167471.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02396
    Clone ID Related Accession (Same CDS sequence) NM_001042814.2 , NM_080114.4 , NM_167471.5 , NM_206744.3 , NM_001042813.2 , NM_206745.3
    Accession Version NM_206744.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206744.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996467.1
    CDS230..2722
    Translation

    Target ORF information:

    RefSeq Version NM_206744.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206744.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02396
    Clone ID Related Accession (Same CDS sequence) NM_001042814.2 , NM_080114.4 , NM_167471.5 , NM_206744.3 , NM_001042813.2 , NM_206745.3
    Accession Version NM_001042813.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001042813.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036278.1
    CDS548..3040
    Translation

    Target ORF information:

    RefSeq Version NM_001042813.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042813.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02396
    Clone ID Related Accession (Same CDS sequence) NM_001042814.2 , NM_080114.4 , NM_167471.5 , NM_206744.3 , NM_001042813.2 , NM_206745.3
    Accession Version NM_206745.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206745.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996468.1
    CDS229..2721
    Translation

    Target ORF information:

    RefSeq Version NM_206745.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206745.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGTCGGCCAG CGAGGAGAGT
    TCCAGCGATC CACAGCTGGA GCGGCAGGTC GAGACCATTC GTAATCTGGT TGATTCCTAC
    ATGAAGATCG TTACGAAGAC CACCCGTGAC ATGGTGCCCA AAGCAATTAT GATGCTGATC
    ATCAACAATG CCAAGGACTT TATCAATGGC GAGCTGCTGG CACATCTTTA CGCGTCCGGC
    GATCAGGCCC AGATGATGGA GGAGTCGGCT GAATCGGCAA CACGGCGAGA GGAAATGCTG
    CGCATGTACC GTGCTTGCAA GGATGCATTA CAGATAATAG GTGACGTATC AATGGCCACC
    GTGTCGTCGC CATTGCCACC GCCGGTTAAG AACGATTGGC TGCCCAGCGG CTTGGACAAC
    CCACGTCTTT CTCCTCCAAG TCCGGGTGGC GTTCGAGGAA AGCCAGGACC TCCCGCACAG
    AGCTCCTTGG GTGGACGGAA CCCACCATTG CCACCATCTA CAGGTCGCCC AGCGCCAGCG
    ATTCCGAATC GCCCAGGAGG TGGGGCACCA CCACTACCCG GCGGTCGTCC CGGAGGATCT
    CTTCCCCCAC CAATGCTACC ATCGCGTCGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02744
    Clone ID Related Accession (Same CDS sequence) NM_001272659.2
    Accession Version NM_001272659.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272659.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGAT ATAAAATTAG GACGATCTGG ACCGCTAAAC
    GTACGATTTT TCAGTCCTTC TACAGGTGGA TCGCGTCCGT ATTGGTTTGT GCTGACATCC
    GAGAGTATCT CCTGGTATAA GGACGAGGAT GAGAAGGAGA AGAAATTTAT GCTGCCGTTG
    GATGGTTTAA AATTGCGTGA TATTGAACAG GGATTTATGT CAATGTCTAG ACGTGTTACA
    TTTGCTTTAT TTAGTCCCGA TGGACGTAAT GTTTATAAGG ACTACAAACA ACTGGAGCTC
    TCGTGCGAGA CTGTGGAGGA TGTGGAGTCC TGGAAAGCAT CCTTCCTGCG AGCTGGTGTC
    TATCCCGAAA AGCAGGAGAC TCAGGAAAAC GGCGATGAGT CGGCCAGCGA GGAGAGTTCC
    AGCGATCCAC AGCTGGAGCG GCAGGTCGAG ACCATTCGTA ATCTGGTTGA TTCCTACATG
    AAGATCGTTA CGAAGACCAC CCGTGACATG GTGCCCAAAG CAATTATGAT GCTGATCATC
    AACAATGCCA AGGACTTTAT CAATGGCGAG CTGCTGGCAC ATCTTTACGC GTCCGGCGAT
    CAGGCCCAGA TGATGGAGGA GTCGGCTGAA TCGGCAACAC GGCGAGAGGA AATGCTGCGC
    ATGTACCGTG CTTGCAAGGA TGCATTACAG ATAATAGGTG ACGTATCAAT GGCCACCGTG
    TCGTCGCCAT TGCCACCGCC GGTTAAGAAC GATTGGCTGC CCAGCGGCTT GGACAACCCA
    CGTCTTTCTC CTCCAAGTCC GGGTGGCGTT CGAGGAAAGC CAGGACCTCC CGCACAGAGC
    TCCTTGGGTG GACGGAACCC ACCATTGCCA CCATCTACAG GTCGCCCAGC GCCAGCGATT
    CCGAATCGCC CAGGAGGTGG GGCACCACCA CTACCCGGCG GTCGTCCCGG AGGATCTCTT
    CCCCCACCAA TGCTACCATC CCGTGTGTCC GGAGCTGTCG GCGGCGCCAT TGTCCAGCAA
    TCGGGAGCCA ATCGCTATGT GCCGGAGAGT ATGCGTGGAC AGGTGAACCA GGCGGTGGGC
    CAGGCAGCCA TCAATGAGTT GTCCAATGCC TTCAGTTCGC GATTCAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259588.1
    CDS230..2920
    Translation

    Target ORF information:

    RefSeq Version NM_001272659.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272659.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGAT ATAAAATTAG GACGATCTGG ACCGCTAAAC
    GTACGATTTT TCAGTCCTTC TACAGGTGGA TCGCGTCCGT ATTGGTTTGT GCTGACATCC
    GAGAGTATCT CCTGGTATAA GGACGAGGAT GAGAAGGAGA AGAAATTTAT GCTGCCGTTG
    GATGGTTTAA AATTGCGTGA TATTGAACAG GGATTTATGT CAATGTCTAG ACGTGTTACA
    TTTGCTTTAT TTAGTCCCGA TGGACGTAAT GTTTATAAGG ACTACAAACA ACTGGAGCTC
    TCGTGCGAGA CTGTGGAGGA TGTGGAGTCC TGGAAAGCAT CCTTCCTGCG AGCTGGTGTC
    TATCCCGAAA AGCAGGAGAC TCAGGAAAAC GGCGATGAGT CGGCCAGCGA GGAGAGTTCC
    AGCGATCCAC AGCTGGAGCG GCAGGTCGAG ACCATTCGTA ATCTGGTTGA TTCCTACATG
    AAGATCGTTA CGAAGACCAC CCGTGACATG GTGCCCAAAG CAATTATGAT GCTGATCATC
    AACAATGCCA AGGACTTTAT CAATGGCGAG CTGCTGGCAC ATCTTTACGC GTCCGGCGAT
    CAGGCCCAGA TGATGGAGGA GTCGGCTGAA TCGGCAACAC GGCGAGAGGA AATGCTGCGC
    ATGTACCGTG CTTGCAAGGA TGCATTACAG ATAATAGGTG ACGTATCAAT GGCCACCGTG
    TCGTCGCCAT TGCCACCGCC GGTTAAGAAC GATTGGCTGC CCAGCGGCTT GGACAACCCA
    CGTCTTTCTC CTCCAAGTCC GGGTGGCGTT CGAGGAAAGC CAGGACCTCC CGCACAGAGC
    TCCTTGGGTG GACGGAACCC ACCATTGCCA CCATCTACAG GTCGCCCAGC GCCAGCGATT
    CCGAATCGCC CAGGAGGTGG GGCACCACCA CTACCCGGCG GTCGTCCCGG AGGATCTCTT
    CCCCCACCAA TGCTACCATC CCGTGTGTCC GGAGCTGTCG GCGGCGCCAT TGTCCAGCAA
    TCGGGAGCCA ATCGCTATGT GCCGGAGAGT ATGCGTGGAC AGGTGAACCA GGCGGTGGGC
    CAGGCAGCCA TCAATGAGTT GTCCAATGCC TTCAGTTCGC GATTCAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02745
    Clone ID Related Accession (Same CDS sequence) NM_001272660.2
    Accession Version NM_001272660.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272660.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGTCGGCC
    AGCGAGGAGA GTTCCAGCGA TCCACAGCTG GAGCGGCAGG TCGAGACCAT TCGTAATCTG
    GTTGATTCCT ACATGAAGAT CGTTACGAAG ACCACCCGTG ACATGGTGCC CAAAGCAATT
    ATGATGCTGA TCATCAACAA TGCCAAGGAC TTTATCAATG GCGAGCTGCT GGCACATCTT
    TACGCGTCCG GCGATCAGGC CCAGATGATG GAGGAGTCGG CTGAATCGGC AACACGGCGA
    GAGGAAATGC TGCGCATGTA CCGTGCTTGC AAGGATGCAT TACAGATAAT AGGTGACGTA
    TCAATGGCCA CCGTGTCGTC GCCATTGCCA CCGCCGGTTA AGAACGATTG GCTGCCCAGC
    GGCTTGGACA ACCCACGTCT TTCTCCTCCA AGTCCGGGTG GCGTTCGAGG AAAGCCAGGA
    CCTCCCGCAC AGAGCTCCTT GGGTGGACGG AACCCACCAT TGCCACCATC TACAGGTCGC
    CCAGCGCCAG CGATTCCGAA TCGCCCAGGA GGTGGGGCAC CACCACTACC CGGCGGTCGT
    CCCGGAGGAT CTCTTCCCCC ACCAATGCTA CCATCGCGTC GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259589.1
    CDS396..2900
    Translation

    Target ORF information:

    RefSeq Version NM_001272660.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272660.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGTCGGCC
    AGCGAGGAGA GTTCCAGCGA TCCACAGCTG GAGCGGCAGG TCGAGACCAT TCGTAATCTG
    GTTGATTCCT ACATGAAGAT CGTTACGAAG ACCACCCGTG ACATGGTGCC CAAAGCAATT
    ATGATGCTGA TCATCAACAA TGCCAAGGAC TTTATCAATG GCGAGCTGCT GGCACATCTT
    TACGCGTCCG GCGATCAGGC CCAGATGATG GAGGAGTCGG CTGAATCGGC AACACGGCGA
    GAGGAAATGC TGCGCATGTA CCGTGCTTGC AAGGATGCAT TACAGATAAT AGGTGACGTA
    TCAATGGCCA CCGTGTCGTC GCCATTGCCA CCGCCGGTTA AGAACGATTG GCTGCCCAGC
    GGCTTGGACA ACCCACGTCT TTCTCCTCCA AGTCCGGGTG GCGTTCGAGG AAAGCCAGGA
    CCTCCCGCAC AGAGCTCCTT GGGTGGACGG AACCCACCAT TGCCACCATC TACAGGTCGC
    CCAGCGCCAG CGATTCCGAA TCGCCCAGGA GGTGGGGCAC CACCACTACC CGGCGGTCGT
    CCCGGAGGAT CTCTTCCCCC ACCAATGCTA CCATCGCGTC GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02746
    Clone ID Related Accession (Same CDS sequence) NM_001169297.2
    Accession Version NM_001169297.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169297.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGCAAAAA
    TCGGCCAGCG AGGAGAGTTC CAGCGATCCA CAGCTGGAGC GGCAGGTCGA GACCATTCGT
    AATCTGGTTG ATTCCTACAT GAAGATCGTT ACGAAGACCA CCCGTGACAT GGTGCCCAAA
    GCAATTATGA TGCTGATCAT CAACAATGCC AAGGACTTTA TCAATGGCGA GCTGCTGGCA
    CATCTTTACG CGTCCGGCGA TCAGGCCCAG ATGATGGAGG AGTCGGCTGA ATCGGCAACA
    CGGCGAGAGG AAATGCTGCG CATGTACCGT GCTTGCAAGG ATGCATTACA GATAATAGGT
    GACGTATCAA TGGCCACCGT GTCGTCGCCA TTGCCACCGC CGGTTAAGAA CGATTGGCTG
    CCCAGCGGCT TGGACAACCC ACGTCTTTCT CCTCCAAGTC CGGGTGGCGT TCGAGGAAAG
    CCAGGACCTC CCGCACAGAG CTCCTTGGGT GGACGGAACC CACCATTGCC ACCATCTACA
    GGTCGCCCAG CGCCAGCGAT TCCGAATCGC CCAGGAGGTG GGGCACCACC ACTACCCGGC
    GGTCGTCCCG GAGGATCTCT TCCCCCACCA ATGCTACCAT CGCGTCGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162768.1
    CDS419..2929
    Translation

    Target ORF information:

    RefSeq Version NM_001169297.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169297.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGCAAAAA
    TCGGCCAGCG AGGAGAGTTC CAGCGATCCA CAGCTGGAGC GGCAGGTCGA GACCATTCGT
    AATCTGGTTG ATTCCTACAT GAAGATCGTT ACGAAGACCA CCCGTGACAT GGTGCCCAAA
    GCAATTATGA TGCTGATCAT CAACAATGCC AAGGACTTTA TCAATGGCGA GCTGCTGGCA
    CATCTTTACG CGTCCGGCGA TCAGGCCCAG ATGATGGAGG AGTCGGCTGA ATCGGCAACA
    CGGCGAGAGG AAATGCTGCG CATGTACCGT GCTTGCAAGG ATGCATTACA GATAATAGGT
    GACGTATCAA TGGCCACCGT GTCGTCGCCA TTGCCACCGC CGGTTAAGAA CGATTGGCTG
    CCCAGCGGCT TGGACAACCC ACGTCTTTCT CCTCCAAGTC CGGGTGGCGT TCGAGGAAAG
    CCAGGACCTC CCGCACAGAG CTCCTTGGGT GGACGGAACC CACCATTGCC ACCATCTACA
    GGTCGCCCAG CGCCAGCGAT TCCGAATCGC CCAGGAGGTG GGGCACCACC ACTACCCGGC
    GGTCGTCCCG GAGGATCTCT TCCCCCACCA ATGCTACCAT CGCGTCGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa02748
    Clone ID Related Accession (Same CDS sequence) NM_001169296.1
    Accession Version NM_001169296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGCAAAAA
    TCGGCCAGCG AGGAGAGTTC CAGCGATCCA CAGCTGGAGC GGCAGGTCGA GACCATTCGT
    AATCTGGTTG ATTCCTACAT GAAGATCGTT ACGAAGACCA CCCGTGACAT GGTGCCCAAA
    GCAATTATGA TGCTGATCAT CAACAATGCC AAGGACTTTA TCAATGGCGA GCTGCTGGCA
    CATCTTTACG CGTCCGGCGA TCAGGCCCAG ATGATGGAGG AGTCGGCTGA ATCGGCAACA
    CGGCGAGAGG AAATGCTGCG CATGTACCGT GCTTGCAAGG ATGCATTACA GATAATAGGT
    GACGTATCAA TGGCCACCGT GTCGTCGCCA TTGCCACCGC CGGTTAAGAA CGATTGGCTG
    CCCAGCGGCT TGGACAACCC ACGTCTTTCT CCTCCAAGTC CGGGTGGCGT TCGAGGAAAG
    CCAGGACCTC CCGCACAGAG CTCCTTGGGT GGACGGAACC CACCATTGCC ACCATCTACA
    GGTCGCCCAG CGCCAGCGAT TCCGAATCGC CCAGGAGGTG GGGCACCACC ACTACCCGGC
    GGTCGTCCCG GAGGATCTCT TCCCCCACCA ATGCTACCAT CCCGTGTGTC CGGAGCTGTC
    GGCGGCGCCA TTGTCCAGCA ATCGGGAGCC AATCGCTATG TGCCGGAGAG TATGCGTGGA
    CAGGTGAACC AGGCGGTGGG CCAGGCAGCC ATCAATGAGT TGTCCAATGC CTTCAGTTCG
    CGATTCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162767.1
    CDS117..2768
    Translation

    Target ORF information:

    RefSeq Version NM_001169296.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGCAAAAA
    TCGGCCAGCG AGGAGAGTTC CAGCGATCCA CAGCTGGAGC GGCAGGTCGA GACCATTCGT
    AATCTGGTTG ATTCCTACAT GAAGATCGTT ACGAAGACCA CCCGTGACAT GGTGCCCAAA
    GCAATTATGA TGCTGATCAT CAACAATGCC AAGGACTTTA TCAATGGCGA GCTGCTGGCA
    CATCTTTACG CGTCCGGCGA TCAGGCCCAG ATGATGGAGG AGTCGGCTGA ATCGGCAACA
    CGGCGAGAGG AAATGCTGCG CATGTACCGT GCTTGCAAGG ATGCATTACA GATAATAGGT
    GACGTATCAA TGGCCACCGT GTCGTCGCCA TTGCCACCGC CGGTTAAGAA CGATTGGCTG
    CCCAGCGGCT TGGACAACCC ACGTCTTTCT CCTCCAAGTC CGGGTGGCGT TCGAGGAAAG
    CCAGGACCTC CCGCACAGAG CTCCTTGGGT GGACGGAACC CACCATTGCC ACCATCTACA
    GGTCGCCCAG CGCCAGCGAT TCCGAATCGC CCAGGAGGTG GGGCACCACC ACTACCCGGC
    GGTCGTCCCG GAGGATCTCT TCCCCCACCA ATGCTACCAT CCCGTGTGTC CGGAGCTGTC
    GGCGGCGCCA TTGTCCAGCA ATCGGGAGCC AATCGCTATG TGCCGGAGAG TATGCGTGGA
    CAGGTGAACC AGGCGGTGGG CCAGGCAGCC ATCAATGAGT TGTCCAATGC CTTCAGTTCG
    CGATTCAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22252
    Clone ID Related Accession (Same CDS sequence) NM_001298377.1
    Accession Version NM_001298377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product shibire, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGTCGGCC
    AGCGAGGAGA GTTCCAGCGA TCCACAGCTG GAGCGGCAGG TCGAGACCAT TCGTAATCTG
    GTTGATTCCT ACATGAAGAT CGTTACGAAG ACCACCCGTG ACATGGTGCC CAAAGCAATT
    ATGATGCTGA TCATCAACAA TGCCAAGGAC TTTATCAATG GCGAGCTGCT GGCACATCTT
    TACGCGTCCG GCGATCAGGC CCAGATGATG GAGGAGTCGG CTGAATCGGC AACACGGCGA
    GAGGAAATGC TGCGCATGTA CCGTGCTTGC AAGGATGCAT TACAGATAAT AGGTGACGTA
    TCAATGGCCA CCGTGTCGTC GCCATTGCCA CCGCCGGTTA AGAACGATTG GCTGCCCAGC
    GGCTTGGACA ACCCACGTCT TTCTCCTCCA AGTCCGGGTG GCGTTCGAGG AAAGCCAGGA
    CCTCCCGCAC AGAGCTCCTT GGGTGGACGG AACCCACCAT TGCCACCATC TACAGGTCGC
    CCAGCGCCAG CGATTCCGAA TCGCCCAGGA GGTGGGGCAC CACCACTACC CGGCGGTCGT
    CCCGGAGGAT CTCTTCCCCC ACCAATGCTA CCATCCCGTG TGTCCGGAGC TGTCGGCGGC
    GCCATTGTCC AGCAATCGGG AGCCAATCGC TATGTGCCGG AGAGTATGCG TGGACAGGTG
    AACCAGGCGG TGGGCCAGGC AGCCATCAAT GAGTTGTCCA ATGCCTTCAG TTCGCGATTC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285306.1
    CDS230..2875
    Translation

    Target ORF information:

    RefSeq Version NM_001298377.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster shibire (shi), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGATAGTT TAATTACAAT TGTTAACAAG CTGCAAGATG CGTTCACATC GCTCGGCGTC 
    CACATGCAAC TGGATCTGCC GCAGATCGCG GTGGTCGGTG GCCAGTCAGC TGGCAAGAGT
    TCCGTTTTGG AGAACTTTGT GGGCAAAGAT TTCTTGCCGC GCGGCTCTGG TATCGTAACT
    CGGAGACCAC TAATCTTGCA ACTGATCAAT GGAGTTACCG AATATGGCGA GTTCCTTCAC
    ATTAAAGGCA AGAAGTTTTC CAGCTTCGAT GAGATCAGAA AAGAAATCGA AGATGAAACG
    GATCGTGTGA CCGGTAGTAA CAAAGGCATT TCAAACATTC CAATTAATTT GCGCGTCTAC
    TCGCCGCACG TACTCAATTT AACCCTGATC GATTTGCCAG GCCTGACAAA GGTGGCCATT
    GGCGATCAAC CGGTGGATAT TGAGCAGCAA ATTAAGCAGA TGATATTCCA ATTTATTCGC
    AAAGAGACTT GTTTAATTTT GGCTGTGACC CCGGCCAATA CGGATCTGGC CAATTCGGAT
    GCCCTCAAGC TGGCCAAGGA GGTGGATCCA CAGGGTGTGC GAACCATTGG CGTTATAACC
    AAGCTGGATC TGATGGACGA GGGCACCGAT GCACGCGACA TTCTGGAGAA TAAGCTGCTT
    CCGCTGCGAC GTGGCTATAT TGGCGTGGTG AATAGATCCC AAAAGGATAT CGAGGGGCGC
    AAGGATATCC ACCAGGCATT GGCCGCCGAA CGCAAGTTCT TCTTGAGCCA TCCCTCCTAT
    CGGCACATGG CCGACCGTCT CGGCACCCCC TACTTGCAGC GCGTGCTCAA CCAACAACTA
    ACCAATCACA TCAGGGACAC GTTACCTGGC CTGCGGGATA AGTTGCAGAA GCAGATGCTC
    ACTTTGGAGA AGGAGGTGGA GGAGTTTAAG CACTTTCAGC CAGGCGATGC TAGCATCAAA
    ACAAAAGCTA TGCTCCAAAT GATTCAGCAG CTGCAATCAG ACTTTGAGCG AACTATCGAG
    GGCAGCGGCT CAGCTTTGGT GAACACAAAC GAGCTCTCAG GTGGCGCCAA GATCAATCGC
    ATTTTCCATG AACGTCTGCG CTTTGAGATC GTAAAGATGG CTTGCGATGA AAAGGAACTG
    CGACGAGAGA TCTCATTTGC AATTCGAAAT ATCCATGGTA TTCGCGTTGG CCTCTTCACA
    CCCGACATGG CGTTCGAGGC GATTGTCAAG CGGCAGATAG CGCTGCTCAA GGAGCCGGTT
    ATCAAGTGTG TCGATCTAGT CGTACAGGAA CTTTCTGTGG TCGTGCGCAT GTGCACAGCT
    AAAATGAGTC GATACCCACG TCTGCGTGAG GAGACCGAAC GTATTATCAC AACACATGTG
    CGCCAACGCG AGCATAGCTG CAAGGAACAG ATCCTGCTGC TTATTGACTT TGAGCTGGCC
    TATATGAACA CCAATCATGA GGACTTCATT GGCTTCGCCA ACGCTCAAAA CAAATCAGAG
    AATGCCAACA AAACTGGCAC TCGTCAACTG GGCAACCAAG TCATTCGCAA GGGTCACATG
    GTCATCCAGA ACCTTGGAAT CATGAAAGGT GGATCGCGTC CGTATTGGTT TGTGCTGACA
    TCCGAGAGTA TCTCCTGGTA TAAGGACGAG GATGAGAAGG AGAAGAAATT TATGCTGCCG
    TTGGATGGTT TAAAATTGCG TGATATTGAA CAGGGATTTA TGTCAATGTC TAGACGTGTT
    ACATTTGCTT TATTTAGTCC CGATGGACGT AATGTTTATA AGGACTACAA ACAACTGGAG
    CTCTCGTGCG AGACTGTGGA GGATGTGGAG TCCTGGAAAG CATCCTTCCT GCGAGCTGGT
    GTCTATCCCG AAAAGCAGGA GACTCAGGAA AACGGCGATG AGGAAGGACA AGAGTCGGCC
    AGCGAGGAGA GTTCCAGCGA TCCACAGCTG GAGCGGCAGG TCGAGACCAT TCGTAATCTG
    GTTGATTCCT ACATGAAGAT CGTTACGAAG ACCACCCGTG ACATGGTGCC CAAAGCAATT
    ATGATGCTGA TCATCAACAA TGCCAAGGAC TTTATCAATG GCGAGCTGCT GGCACATCTT
    TACGCGTCCG GCGATCAGGC CCAGATGATG GAGGAGTCGG CTGAATCGGC AACACGGCGA
    GAGGAAATGC TGCGCATGTA CCGTGCTTGC AAGGATGCAT TACAGATAAT AGGTGACGTA
    TCAATGGCCA CCGTGTCGTC GCCATTGCCA CCGCCGGTTA AGAACGATTG GCTGCCCAGC
    GGCTTGGACA ACCCACGTCT TTCTCCTCCA AGTCCGGGTG GCGTTCGAGG AAAGCCAGGA
    CCTCCCGCAC AGAGCTCCTT GGGTGGACGG AACCCACCAT TGCCACCATC TACAGGTCGC
    CCAGCGCCAG CGATTCCGAA TCGCCCAGGA GGTGGGGCAC CACCACTACC CGGCGGTCGT
    CCCGGAGGAT CTCTTCCCCC ACCAATGCTA CCATCCCGTG TGTCCGGAGC TGTCGGCGGC
    GCCATTGTCC AGCAATCGGG AGCCAATCGC TATGTGCCGG AGAGTATGCG TGGACAGGTG
    AACCAGGCGG TGGGCCAGGC AGCCATCAAT GAGTTGTCCA ATGCCTTCAG TTCGCGATTC
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.