ImpE1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ImpE1
Entrez Gene ID	45879
Full Name	Ecdysone-inducible gene E1
Synonyms	BcDNA:RE39082,CG13668,CG32356,CG7116,CT22003,CT33103,Dmel\CG32356,IMP-E1,Imp-E1
General protein information	Preferred Names Ecdysone-inducible gene E1 Names CG32356 gene product from transcript CG32356-RC CG32356-PA CG32356-PB CG32356-PC ImpE1-PA ImpE1-PB ImpE1-PC Y1-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66C8-66C10

mRNA and Protein(s)

mRNA	Protein	Name
NM_168270.2	NP_729371.1	Ecdysone-inducible gene E1, isoform B
NM_001274648.1	NP_001261577.1	Ecdysone-inducible gene E1, isoform C
NM_080110.3	NP_524849.2	Ecdysone-inducible gene E1, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ImpE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ImpE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19422	NM_168270.2 Latest version!	Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa19421	NM_080110.3 Latest version!	Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa19421	NM_001274648.1 Latest version!	Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ImpE1 ( NM_168270.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729371.1 Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa19422
Clone ID Related Accession (Same CDS sequence)	NM_168270.2
Accession Version	NM_168270.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-inducible gene E1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168270.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGCGA CTGCGAGGCA GTCGGCAACA TCGAGAAAGC AACCTGCGAT CGCAATGCTC 
AATGGAAACG CTTTTACGGT TAAGCGGCTC TTAGCGCTGC TGATCATCTT CACAGTGGTG 
GACGCGAGTC GATCCCTGGA GCTGGGCGAC AAGTGCCAGC ATGACATGGA CTGCACGGAT 
TTCATCAAGG GCAGCAGCTG CTCCGCCCTC GGATACTGCG AGTGCGCCCC CTACTTCGTC 
CAGTTGGATT CCAAGCGCTG TTTGTCATCC CAGCTCCTTG GCGGCGACTG CCAGCTGAGC 
GAGCAGTGCT CCATGAAGGT GGCCAATAGC AGTTGTCTGG AGGGTGCATG CCGCTGCGTC 
GAGGGATTCC TGCAGTTCCG CAAGCACACC TGTCTGGGAC GTAAGTATCA GCCGACCGCC 
GGCCAGCTAT TGTGGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729371.1
CDS	355..795
Translation	MTATARQSATSRKQPAIAMLNGNAFTVKRLLALLIIFTVVDASRSLELGDKCQHDMDCTDFIKGSSCSALGYCECAPYFVQLDSKRCLSSQLLGGDCQLSEQCSMKVANSSCLEGACRCVEGFLQFRKHTCLGRKYQPTAGQLLWP

Target ORF information:

RefSeq Version	NM_168270.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168270.2

ORF Insert Sequence:

ATGACGGCGA CTGCGAGGCA GTCGGCAACA TCGAGAAAGC AACCTGCGAT CGCAATGCTC 
AATGGAAACG CTTTTACGGT TAAGCGGCTC TTAGCGCTGC TGATCATCTT CACAGTGGTG 
GACGCGAGTC GATCCCTGGA GCTGGGCGAC AAGTGCCAGC ATGACATGGA CTGCACGGAT 
TTCATCAAGG GCAGCAGCTG CTCCGCCCTC GGATACTGCG AGTGCGCCCC CTACTTCGTC 
CAGTTGGATT CCAAGCGCTG TTTGTCATCC CAGCTCCTTG GCGGCGACTG CCAGCTGAGC 
GAGCAGTGCT CCATGAAGGT GGCCAATAGC AGTTGTCTGG AGGGTGCATG CCGCTGCGTC 
GAGGGATTCC TGCAGTTCCG CAAGCACACC TGTCTGGGAC GTAAGTATCA GCCGACCGCC 
GGCCAGCTAT TGTGGCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ImpE1 ( NM_001274648.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261577.1 Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19421
Clone ID Related Accession (Same CDS sequence)	NM_001274648.1 , NM_080110.3
Accession Version	NM_001274648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-inducible gene E1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGCGA CTGCGAGGCA GTCGGCAACA TCGAGAAAGC AACCTGCGAT CGCAATGCTC 
AATGGAAACG CTTTTACGGT TAAGCGGCTC TTAGCGCTGC TGATCATCTT CACAGTGGTG 
GACGCGAGTC GATCCCTGGA GCTGGGCGAC AAGTGCCAGC ATGACATGGA CTGCACGGAT 
TTCATCAAGG GCAGCAGCTG CTCCGCCCTC GGATACTGCG AGTGCGCCCC CTACTTCGTC 
CAGTTGGATT CCAAGCGCTG TTTGTCATCC CAGCTCCTTG GCGGCGACTG CCAGCTGAGC 
GAGCAGTGCT CCATGAAGGT GGCCAATAGC AGTTGTCTGG AGGGTGCATG CCGCTGCGTC 
GAGGGATTCC TGCAGTTCCG CAAGCACACC TGTCTGGGAC CTGCCCATCC TGGTGCCGTT 
TGCTACAGCC ACGCCCACTG TCAAATGTTC GACACCCGCA CCCACTGTGA CTTCCTCATC 
CCGAATCTCT TCGGACGCTG CCAGTGCACA AGTCCAGCCA AAATGGTGGG TGGTCTGTGT 
GTGGCCCCGG CGGCGGATCA GGAGGATCAG AAGCAGGAAC AGGCCGTGGA GAAGGTAGAG 
CAGCCAGCTA GCAGTACCAC TACCACGACG ACAACAACAA CCACTCCTAG GCCCACAACT 
AGAACCACGA GTAGAACAAC GACGACCACC ACTGCAGCAC CACCAACAAC CACCAGAAGA 
ACAACAACGA CCACAACTAC GACAACTCCA GCGCCCATTC TCCTAGAAGA TGAGCTGCCC 
GCCAGCGTGG AGGAGCAATC GCTGGAGGAA GACGATGGTG AGACCACCAA GCTGAGACCC 
GAGGTCGTTG AGGTTGATAA TGGTGAAAAA TTGGAGGCTG TGGACCTGGC TCATGAGGAA 
ACCGAACTGA CACAGCAGCA AAATCAATTA GATGAGCAGC AACATCAGGA GGAGGAACAG 
AAACATCAAG AGGAGGAACA ACATAGGATT GAGCAGCAGC AGAACCAAGA GGAGGAGCAG 
CAGCAGCAGC AGCATCAGCA GCAGGTCATA GACCAAGCAA GCGACGCAGA GCTTCCTCAA 
TCCTCCCAAG ATGCCACCAC TGGAGTCCAA CAGCTGCTCA CACCTGCTGA TGTGCCAACC 
GAGGATGCCA TTAATGCGGA ACAGGAAACT GAGGCTGGAC CCACTGAAGA TTACCCATAC 
AAGATCGATG AGGAGTACAC AGAGGAGCAC AATGACAAGC CAGAAGCTCC AGAACTGACA 
GTGGATCAAC CTGAGCACGA AGAATCCCCA GTAGCCACTG AAATCGTGCC TATCGAAGCG 
GATGAGGAGG CTGCTGAGGC CTCGGGTGGT CAAGAGATTG CCTCATCGCC CATCGACGAT 
ACCATGAAAT TCGATTATGA AACATCAGTG GATGATGTTG AGCCAGCCAA GCCAGAAGAT 
GAAAAGGAGA CGGAGGCAGT ACACCAAGCG ACTAATGAGG GGGTGGTGCC CATTGATACC 
GAAAGCGAGA CTGATGCAGA GACTAGCACC GAGCCCCAGA TCCAGACGCA GCTGCTGCAG 
GAGGATGGAG AGCCAGAGAC ATCGCAGACT GTGGCCGATG AAGAACTGAT CCCGGTTAAT 
GAAGGTGAAA CCGAAAGTGT GACGAAGGCA GCCGACGAGA CCGAGGAGAT ACCGGAGGAG 
GAAACACCTG AGGATCTGGC TTCGGGAGAT CATGAGCTGG ATGTTGCTGC AGCCAGCCAC 
GATGATGAGG TGGAGACGCC CAAGCAGGAG GAGCCAACAG AAGCGTTGAA GATCGAACAG 
CCAGCCCAGG TGGAGAGTGA AAGCCAAAAC GTCGACGAGG TGGGAAAACC AGAGGAGCCA 
CAGGCCGAAA TGGAAATAGA AAAAGAAAAA GAAGAAACGG CAGCGACCGA AGCCGCAGAA 
GTGGAGCTGC CGGCCACAGA AGCCAAACCA GATATGGAAT CCGAGATCGA TCAGGTGGAA 
CAAAGCGCCG ACATCAAGCC GACAAGTGGC GAAGATGATT TAAATGAAGC CAATCAATTA 
AATCAAGACC AAGTCACCGA GCCAGCCGCT CTTGAACAGG AGGAGATCGA AGAAAAAGAG 
CCCGAGCAGG TGGTGGATAT GCATGAAGAT GCAGATGAAG CCACAACGGA GAAACCAGTC 
CAAGATCACG ACCAGGAGGA GGTGGAGGCA ATCACCGAAG CGGAGCACGG ACAATTGACA 
TACCCAACAA ATGATGATGA ATTGGCGCCA GTGGAAATAG AACCAGAGCT GACCACAGAG 
GAACCAGAAA TCGCTGAGAA CACCACCGAG CTGCCCGAGC AACAGCAACA GCAGCAGGAT 
GAGGAGGAGA TTGAAGAGGC GGCACTTGCA CATGAGGAGC AGGTGCAGCA GGTGGAGCCC 
GAGGAAAATG CAAATGCAAT TAAGACGCCC GAAGTGTCGG AAGCAATTGC AGAGCCCCAG 
GCGGATCTAG AAACCCCGTC AGCCACCGAG AAGGAGATCG AACCACAGAC AGCGGACCAT 
GTTGAGGAGG AGCCAGTGGC GATAACCGAG CGCATTCAAG AGGCAGAGCC AGAGCCAGAA 
ATTGAGCCCA CTAATGACGA GGTGTCGCAG GTGGATGCGG ACCAGGCCGA GCCAGAGCTG 
CCAATTAAGA GCGATGCCGA CATCGCGGAG GAAGCCGCCG AGGAACCTGC TCAGACGGAG 
GTGGCTCCCG AAGTGGAAAC GGAAACCGAG GCCAGTGAAA GTGCTCAGAC ATCTCCAGAG 
ATTAGCGTAG AGCAAGATGA AGTTGCGATC GATAAAAACG AGAATGACAA CATTATCCAG 
GGAGCCGAAC CCAATGAAGA GGACGAACTT ATCCCGGAAC CACAACCAAT GCCAGAAGAG 
GAGGAATTGC CAGTTCAACA GGAAGCATCA GCTGTTCCGG AGGATCATAA GTTGACCAAG 
CCAGAAATGG ACTATACCAG CGTGGAGCAT CTACAGGAAC TTTCCCGGCC AGAAGAAACG 
ACTGCTGCTA TTGAAGCTGA AGAATCTGAC TCTCGCGAAT CCACTCTGGC TCCAGAGGCT 
CCAGATGCAC CTATTGCCAT TGTGAGCGAG GAACGCGATC CCGAACATGA TCCTCAGAAC 
TTCCATGAGA GCGAGAGCAT TGCTGATATC CTCAGCGACT TGATGTTGGA GGCTGACAGC 
ACTGTGCCTC CTGTTCCATT TGGTCAGCAA CCCGCCCAAA CGGTTCCCAT GGTGGAGGCC 
AACTCGGAAA ACGAGGAGGA ACAAGCACAT GAAGCCATTT CGGATCTCCA GGCTGAAGCC 
GATGAGACCC AGGAACATGC CGAGCAAGTG CAACCGGTTA ACGAGCCAGG AACTGCTATT 
CCCGAACTGT TTGCAGAGGC AGCTAAGGAT GAAGAAGATG TCCCAGCTCT CCCAGAATCT 
GATGAATCGA AAACCGAACA ACCAAGCGCT CTGGAGGTGG AGCAGCACAT TGCCAGTGAA 
GCGGAGCAAA CGCCAGTGGA AGATGAGCAC CAGTATGATG AAGAAGAGCA CCAGCAGGAT 
CAGGAGGAAC ATCAGCAGGA TGAGGACGAG CCCCTTAAGG AAGTAGAAAC CGAATCCCTG 
CCAGAAGAAG CCACCACAGA AAAGTTCCAG GAGCCCGAGC CCGAAGAAGT TAACACTCCG 
TTGCCCGAGG AACTGCCCTT CGATCTTACA GATCACTCCG ATGTGAACCG CTTCAATGAG 
CTGCAATCCG AAAGTCTAAT TCTGGAATCC ACTACCAATA TCGATGGTCT GCAGGAGCTG 
GACAACAACC ATATTGAGGA AGAGGAGCCA GCAGCTCATG CCATTCCACA TGAGGAGGCC 
ACACCCAATC CCGCTGACAT TGTCGAGATC ACCACACAAA CCATGTTGGG CCTGGCCAGT 
CGTGTGACCC TTATGGAGCC CGCTGCTCCC GTCGTTACCA CGCTAATGCC CCTAATGACC 
GCCGATGAAC CCACGCCGGA ACCCGTTTCT CCAGCAGCCA TTCCTTCCGC CTCTCTGGCT 
GCCATCAAAC CCGGTACGGA GTTGCGCAAG CGCGTGGATT TGGGTCTGGA AGCAGTTTCA 
CTTGGATTGG CCTGCAGCAG TGATCGTCAG TGCCAGTTGG CTGATCCCCA CACCGTGTGC 
AATAGACGAG GAGTCTGCGA TTGTGCTGCC GGAGAAGAGG GATCACAGTG CAGCGCCGAG 
AGAACCGGAT GCAGTCCGGG AACCTTCCAG TGCCGCTCGA GTGGCGTGTG CATCTCGTGG 
TTCTTTGTGT GCGACGGCCG CGCCGACTGC AATGACGCCT CCGACGAGGA GTGCACCCAC 
AACGCCCGCC TCAACCAGAC CTGCCCCACG GAATCCTTCC GCTGCCAGCG AAGCGGTCGC 
TGCATCTCGC GCGCTGCGCT GTGCGACGGA CGACGGCAGT GTCCACATGG CGAGGACGAG 
CTGGGCTGCG ATGGCAGCGT GAAGGGCGGC AACGCCTGTC CGGAACACAC CTTCCGCTGC 
GGCAGCGGCG AGTGCCTGCC GGAGTACGAG TACTGTAACG CGATCGTGTC CTGCAAGGAC 
GGCAGCGATG AGCCGCCGCA TCTGTGTGGA TCCCGCGCCC TCCCCAACCT CTTCATGCGC 
CTCATCGAGG CGGGCGGCCT GCTCGGCGGT GGTCGGCGGG AGGCGGACGC CTACTGTCCG 
CACCGCTGCA GCAACGGACT GTGCCGCTCC ACGGCCATCG TCTGCTCCGG ACGGGATGGT 
TGCGGTGATG GCACCGACGA ACAAACCTGC TCCGTGTGCC GCTGTCCCGC CCCGACTGCT 
GCCAGCCTGC CCGCATTCTT GGCCCGACAA CGCCCCATGC CGCTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261577.1
CDS	411..5261
Translation	MTATARQSATSRKQPAIAMLNGNAFTVKRLLALLIIFTVVDASRSLELGDKCQHDMDCTDFIKGSSCSALGYCECAPYFVQLDSKRCLSSQLLGGDCQLSEQCSMKVANSSCLEGACRCVEGFLQFRKHTCLGPAHPGAVCYSHAHCQMFDTRTHCDFLIPNLFGRCQCTSPAKMVGGLCVAPAADQEDQKQEQAVEKVEQPASSTTTTTTTTTTPRPTTRTTSRTTTTTTAAPPTTTRRTTTTTTTTTPAPILLEDELPASVEEQSLEEDDGETTKLRPEVVEVDNGEKLEAVDLAHEETELTQQQNQLDEQQHQEEEQKHQEEEQHRIEQQQNQEEEQQQQQHQQQVIDQASDAELPQSSQDATTGVQQLLTPADVPTEDAINAEQETEAGPTEDYPYKIDEEYTEEHNDKPEAPELTVDQPEHEESPVATEIVPIEADEEAAEASGGQEIASSPIDDTMKFDYETSVDDVEPAKPEDEKETEAVHQATNEGVVPIDTESETDAETSTEPQIQTQLLQEDGEPETSQTVADEELIPVNEGETESVTKAADETEEIPEEETPEDLASGDHELDVAAASHDDEVETPKQEEPTEALKIEQPAQVESESQNVDEVGKPEEPQAEMEIEKEKEETAATEAAEVELPATEAKPDMESEIDQVEQSADIKPTSGEDDLNEANQLNQDQVTEPAALEQEEIEEKEPEQVVDMHEDADEATTEKPVQDHDQEEVEAITEAEHGQLTYPTNDDELAPVEIEPELTTEEPEIAENTTELPEQQQQQQDEEEIEEAALAHEEQVQQVEPEENANAIKTPEVSEAIAEPQADLETPSATEKEIEPQTADHVEEEPVAITERIQEAEPEPEIEPTNDEVSQVDADQAEPELPIKSDADIAEEAAEEPAQTEVAPEVETETEASESAQTSPEISVEQDEVAIDKNENDNIIQGAEPNEEDELIPEPQPMPEEEELPVQQEASAVPEDHKLTKPEMDYTSVEHLQELSRPEETTAAIEAEESDSRESTLAPEAPDAPIAIVSEERDPEHDPQNFHESESIADILSDLMLEADSTVPPVPFGQQPAQTVPMVEANSENEEEQAHEAISDLQAEADETQEHAEQVQPVNEPGTAIPELFAEAAKDEEDVPALPESDESKTEQPSALEVEQHIASEAEQTPVEDEHQYDEEEHQQDQEEHQQDEDEPLKEVETESLPEEATTEKFQEPEPEEVNTPLPEELPFDLTDHSDVNRFNELQSESLILESTTNIDGLQELDNNHIEEEEPAAHAIPHEEATPNPADIVEITTQTMLGLASRVTLMEPAAPVVTTLMPLMTADEPTPEPVSPAAIPSASLAAIKPGTELRKRVDLGLEAVSLGLACSSDRQCQLADPHTVCNRRGVCDCAAGEEGSQCSAERTGCSPGTFQCRSSGVCISWFFVCDGRADCNDASDEECTHNARLNQTCPTESFRCQRSGRCISRAALCDGRRQCPHGEDELGCDGSVKGGNACPEHTFRCGSGECLPEYEYCNAIVSCKDGSDEPPHLCGSRALPNLFMRLIEAGGLLGGGRREADAYCPHRCSNGLCRSTAIVCSGRDGCGDGTDEQTCSVCRCPAPTAASLPAFLARQRPMPLW

Target ORF information:

RefSeq Version	NM_001274648.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274648.1

ORF Insert Sequence:

ATGACGGCGA CTGCGAGGCA GTCGGCAACA TCGAGAAAGC AACCTGCGAT CGCAATGCTC 
AATGGAAACG CTTTTACGGT TAAGCGGCTC TTAGCGCTGC TGATCATCTT CACAGTGGTG 
GACGCGAGTC GATCCCTGGA GCTGGGCGAC AAGTGCCAGC ATGACATGGA CTGCACGGAT 
TTCATCAAGG GCAGCAGCTG CTCCGCCCTC GGATACTGCG AGTGCGCCCC CTACTTCGTC 
CAGTTGGATT CCAAGCGCTG TTTGTCATCC CAGCTCCTTG GCGGCGACTG CCAGCTGAGC 
GAGCAGTGCT CCATGAAGGT GGCCAATAGC AGTTGTCTGG AGGGTGCATG CCGCTGCGTC 
GAGGGATTCC TGCAGTTCCG CAAGCACACC TGTCTGGGAC CTGCCCATCC TGGTGCCGTT 
TGCTACAGCC ACGCCCACTG TCAAATGTTC GACACCCGCA CCCACTGTGA CTTCCTCATC 
CCGAATCTCT TCGGACGCTG CCAGTGCACA AGTCCAGCCA AAATGGTGGG TGGTCTGTGT 
GTGGCCCCGG CGGCGGATCA GGAGGATCAG AAGCAGGAAC AGGCCGTGGA GAAGGTAGAG 
CAGCCAGCTA GCAGTACCAC TACCACGACG ACAACAACAA CCACTCCTAG GCCCACAACT 
AGAACCACGA GTAGAACAAC GACGACCACC ACTGCAGCAC CACCAACAAC CACCAGAAGA 
ACAACAACGA CCACAACTAC GACAACTCCA GCGCCCATTC TCCTAGAAGA TGAGCTGCCC 
GCCAGCGTGG AGGAGCAATC GCTGGAGGAA GACGATGGTG AGACCACCAA GCTGAGACCC 
GAGGTCGTTG AGGTTGATAA TGGTGAAAAA TTGGAGGCTG TGGACCTGGC TCATGAGGAA 
ACCGAACTGA CACAGCAGCA AAATCAATTA GATGAGCAGC AACATCAGGA GGAGGAACAG 
AAACATCAAG AGGAGGAACA ACATAGGATT GAGCAGCAGC AGAACCAAGA GGAGGAGCAG 
CAGCAGCAGC AGCATCAGCA GCAGGTCATA GACCAAGCAA GCGACGCAGA GCTTCCTCAA 
TCCTCCCAAG ATGCCACCAC TGGAGTCCAA CAGCTGCTCA CACCTGCTGA TGTGCCAACC 
GAGGATGCCA TTAATGCGGA ACAGGAAACT GAGGCTGGAC CCACTGAAGA TTACCCATAC 
AAGATCGATG AGGAGTACAC AGAGGAGCAC AATGACAAGC CAGAAGCTCC AGAACTGACA 
GTGGATCAAC CTGAGCACGA AGAATCCCCA GTAGCCACTG AAATCGTGCC TATCGAAGCG 
GATGAGGAGG CTGCTGAGGC CTCGGGTGGT CAAGAGATTG CCTCATCGCC CATCGACGAT 
ACCATGAAAT TCGATTATGA AACATCAGTG GATGATGTTG AGCCAGCCAA GCCAGAAGAT 
GAAAAGGAGA CGGAGGCAGT ACACCAAGCG ACTAATGAGG GGGTGGTGCC CATTGATACC 
GAAAGCGAGA CTGATGCAGA GACTAGCACC GAGCCCCAGA TCCAGACGCA GCTGCTGCAG 
GAGGATGGAG AGCCAGAGAC ATCGCAGACT GTGGCCGATG AAGAACTGAT CCCGGTTAAT 
GAAGGTGAAA CCGAAAGTGT GACGAAGGCA GCCGACGAGA CCGAGGAGAT ACCGGAGGAG 
GAAACACCTG AGGATCTGGC TTCGGGAGAT CATGAGCTGG ATGTTGCTGC AGCCAGCCAC 
GATGATGAGG TGGAGACGCC CAAGCAGGAG GAGCCAACAG AAGCGTTGAA GATCGAACAG 
CCAGCCCAGG TGGAGAGTGA AAGCCAAAAC GTCGACGAGG TGGGAAAACC AGAGGAGCCA 
CAGGCCGAAA TGGAAATAGA AAAAGAAAAA GAAGAAACGG CAGCGACCGA AGCCGCAGAA 
GTGGAGCTGC CGGCCACAGA AGCCAAACCA GATATGGAAT CCGAGATCGA TCAGGTGGAA 
CAAAGCGCCG ACATCAAGCC GACAAGTGGC GAAGATGATT TAAATGAAGC CAATCAATTA 
AATCAAGACC AAGTCACCGA GCCAGCCGCT CTTGAACAGG AGGAGATCGA AGAAAAAGAG 
CCCGAGCAGG TGGTGGATAT GCATGAAGAT GCAGATGAAG CCACAACGGA GAAACCAGTC 
CAAGATCACG ACCAGGAGGA GGTGGAGGCA ATCACCGAAG CGGAGCACGG ACAATTGACA 
TACCCAACAA ATGATGATGA ATTGGCGCCA GTGGAAATAG AACCAGAGCT GACCACAGAG 
GAACCAGAAA TCGCTGAGAA CACCACCGAG CTGCCCGAGC AACAGCAACA GCAGCAGGAT 
GAGGAGGAGA TTGAAGAGGC GGCACTTGCA CATGAGGAGC AGGTGCAGCA GGTGGAGCCC 
GAGGAAAATG CAAATGCAAT TAAGACGCCC GAAGTGTCGG AAGCAATTGC AGAGCCCCAG 
GCGGATCTAG AAACCCCGTC AGCCACCGAG AAGGAGATCG AACCACAGAC AGCGGACCAT 
GTTGAGGAGG AGCCAGTGGC GATAACCGAG CGCATTCAAG AGGCAGAGCC AGAGCCAGAA 
ATTGAGCCCA CTAATGACGA GGTGTCGCAG GTGGATGCGG ACCAGGCCGA GCCAGAGCTG 
CCAATTAAGA GCGATGCCGA CATCGCGGAG GAAGCCGCCG AGGAACCTGC TCAGACGGAG 
GTGGCTCCCG AAGTGGAAAC GGAAACCGAG GCCAGTGAAA GTGCTCAGAC ATCTCCAGAG 
ATTAGCGTAG AGCAAGATGA AGTTGCGATC GATAAAAACG AGAATGACAA CATTATCCAG 
GGAGCCGAAC CCAATGAAGA GGACGAACTT ATCCCGGAAC CACAACCAAT GCCAGAAGAG 
GAGGAATTGC CAGTTCAACA GGAAGCATCA GCTGTTCCGG AGGATCATAA GTTGACCAAG 
CCAGAAATGG ACTATACCAG CGTGGAGCAT CTACAGGAAC TTTCCCGGCC AGAAGAAACG 
ACTGCTGCTA TTGAAGCTGA AGAATCTGAC TCTCGCGAAT CCACTCTGGC TCCAGAGGCT 
CCAGATGCAC CTATTGCCAT TGTGAGCGAG GAACGCGATC CCGAACATGA TCCTCAGAAC 
TTCCATGAGA GCGAGAGCAT TGCTGATATC CTCAGCGACT TGATGTTGGA GGCTGACAGC 
ACTGTGCCTC CTGTTCCATT TGGTCAGCAA CCCGCCCAAA CGGTTCCCAT GGTGGAGGCC 
AACTCGGAAA ACGAGGAGGA ACAAGCACAT GAAGCCATTT CGGATCTCCA GGCTGAAGCC 
GATGAGACCC AGGAACATGC CGAGCAAGTG CAACCGGTTA ACGAGCCAGG AACTGCTATT 
CCCGAACTGT TTGCAGAGGC AGCTAAGGAT GAAGAAGATG TCCCAGCTCT CCCAGAATCT 
GATGAATCGA AAACCGAACA ACCAAGCGCT CTGGAGGTGG AGCAGCACAT TGCCAGTGAA 
GCGGAGCAAA CGCCAGTGGA AGATGAGCAC CAGTATGATG AAGAAGAGCA CCAGCAGGAT 
CAGGAGGAAC ATCAGCAGGA TGAGGACGAG CCCCTTAAGG AAGTAGAAAC CGAATCCCTG 
CCAGAAGAAG CCACCACAGA AAAGTTCCAG GAGCCCGAGC CCGAAGAAGT TAACACTCCG 
TTGCCCGAGG AACTGCCCTT CGATCTTACA GATCACTCCG ATGTGAACCG CTTCAATGAG 
CTGCAATCCG AAAGTCTAAT TCTGGAATCC ACTACCAATA TCGATGGTCT GCAGGAGCTG 
GACAACAACC ATATTGAGGA AGAGGAGCCA GCAGCTCATG CCATTCCACA TGAGGAGGCC 
ACACCCAATC CCGCTGACAT TGTCGAGATC ACCACACAAA CCATGTTGGG CCTGGCCAGT 
CGTGTGACCC TTATGGAGCC CGCTGCTCCC GTCGTTACCA CGCTAATGCC CCTAATGACC 
GCCGATGAAC CCACGCCGGA ACCCGTTTCT CCAGCAGCCA TTCCTTCCGC CTCTCTGGCT 
GCCATCAAAC CCGGTACGGA GTTGCGCAAG CGCGTGGATT TGGGTCTGGA AGCAGTTTCA 
CTTGGATTGG CCTGCAGCAG TGATCGTCAG TGCCAGTTGG CTGATCCCCA CACCGTGTGC 
AATAGACGAG GAGTCTGCGA TTGTGCTGCC GGAGAAGAGG GATCACAGTG CAGCGCCGAG 
AGAACCGGAT GCAGTCCGGG AACCTTCCAG TGCCGCTCGA GTGGCGTGTG CATCTCGTGG 
TTCTTTGTGT GCGACGGCCG CGCCGACTGC AATGACGCCT CCGACGAGGA GTGCACCCAC 
AACGCCCGCC TCAACCAGAC CTGCCCCACG GAATCCTTCC GCTGCCAGCG AAGCGGTCGC 
TGCATCTCGC GCGCTGCGCT GTGCGACGGA CGACGGCAGT GTCCACATGG CGAGGACGAG 
CTGGGCTGCG ATGGCAGCGT GAAGGGCGGC AACGCCTGTC CGGAACACAC CTTCCGCTGC 
GGCAGCGGCG AGTGCCTGCC GGAGTACGAG TACTGTAACG CGATCGTGTC CTGCAAGGAC 
GGCAGCGATG AGCCGCCGCA TCTGTGTGGA TCCCGCGCCC TCCCCAACCT CTTCATGCGC 
CTCATCGAGG CGGGCGGCCT GCTCGGCGGT GGTCGGCGGG AGGCGGACGC CTACTGTCCG 
CACCGCTGCA GCAACGGACT GTGCCGCTCC ACGGCCATCG TCTGCTCCGG ACGGGATGGT 
TGCGGTGATG GCACCGACGA ACAAACCTGC TCCGTGTGCC GCTGTCCCGC CCCGACTGCT 
GCCAGCCTGC CCGCATTCTT GGCCCGACAA CGCCCCATGC CGCTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ImpE1 ( NM_080110.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524849.2 Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant A, mRNA.

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CloneID	OFa19421
Clone ID Related Accession (Same CDS sequence)	NM_001274648.1 , NM_080110.3
Accession Version	NM_080110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-inducible gene E1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080110.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGCGA CTGCGAGGCA GTCGGCAACA TCGAGAAAGC AACCTGCGAT CGCAATGCTC 
AATGGAAACG CTTTTACGGT TAAGCGGCTC TTAGCGCTGC TGATCATCTT CACAGTGGTG 
GACGCGAGTC GATCCCTGGA GCTGGGCGAC AAGTGCCAGC ATGACATGGA CTGCACGGAT 
TTCATCAAGG GCAGCAGCTG CTCCGCCCTC GGATACTGCG AGTGCGCCCC CTACTTCGTC 
CAGTTGGATT CCAAGCGCTG TTTGTCATCC CAGCTCCTTG GCGGCGACTG CCAGCTGAGC 
GAGCAGTGCT CCATGAAGGT GGCCAATAGC AGTTGTCTGG AGGGTGCATG CCGCTGCGTC 
GAGGGATTCC TGCAGTTCCG CAAGCACACC TGTCTGGGAC CTGCCCATCC TGGTGCCGTT 
TGCTACAGCC ACGCCCACTG TCAAATGTTC GACACCCGCA CCCACTGTGA CTTCCTCATC 
CCGAATCTCT TCGGACGCTG CCAGTGCACA AGTCCAGCCA AAATGGTGGG TGGTCTGTGT 
GTGGCCCCGG CGGCGGATCA GGAGGATCAG AAGCAGGAAC AGGCCGTGGA GAAGGTAGAG 
CAGCCAGCTA GCAGTACCAC TACCACGACG ACAACAACAA CCACTCCTAG GCCCACAACT 
AGAACCACGA GTAGAACAAC GACGACCACC ACTGCAGCAC CACCAACAAC CACCAGAAGA 
ACAACAACGA CCACAACTAC GACAACTCCA GCGCCCATTC TCCTAGAAGA TGAGCTGCCC 
GCCAGCGTGG AGGAGCAATC GCTGGAGGAA GACGATGGTG AGACCACCAA GCTGAGACCC 
GAGGTCGTTG AGGTTGATAA TGGTGAAAAA TTGGAGGCTG TGGACCTGGC TCATGAGGAA 
ACCGAACTGA CACAGCAGCA AAATCAATTA GATGAGCAGC AACATCAGGA GGAGGAACAG 
AAACATCAAG AGGAGGAACA ACATAGGATT GAGCAGCAGC AGAACCAAGA GGAGGAGCAG 
CAGCAGCAGC AGCATCAGCA GCAGGTCATA GACCAAGCAA GCGACGCAGA GCTTCCTCAA 
TCCTCCCAAG ATGCCACCAC TGGAGTCCAA CAGCTGCTCA CACCTGCTGA TGTGCCAACC 
GAGGATGCCA TTAATGCGGA ACAGGAAACT GAGGCTGGAC CCACTGAAGA TTACCCATAC 
AAGATCGATG AGGAGTACAC AGAGGAGCAC AATGACAAGC CAGAAGCTCC AGAACTGACA 
GTGGATCAAC CTGAGCACGA AGAATCCCCA GTAGCCACTG AAATCGTGCC TATCGAAGCG 
GATGAGGAGG CTGCTGAGGC CTCGGGTGGT CAAGAGATTG CCTCATCGCC CATCGACGAT 
ACCATGAAAT TCGATTATGA AACATCAGTG GATGATGTTG AGCCAGCCAA GCCAGAAGAT 
GAAAAGGAGA CGGAGGCAGT ACACCAAGCG ACTAATGAGG GGGTGGTGCC CATTGATACC 
GAAAGCGAGA CTGATGCAGA GACTAGCACC GAGCCCCAGA TCCAGACGCA GCTGCTGCAG 
GAGGATGGAG AGCCAGAGAC ATCGCAGACT GTGGCCGATG AAGAACTGAT CCCGGTTAAT 
GAAGGTGAAA CCGAAAGTGT GACGAAGGCA GCCGACGAGA CCGAGGAGAT ACCGGAGGAG 
GAAACACCTG AGGATCTGGC TTCGGGAGAT CATGAGCTGG ATGTTGCTGC AGCCAGCCAC 
GATGATGAGG TGGAGACGCC CAAGCAGGAG GAGCCAACAG AAGCGTTGAA GATCGAACAG 
CCAGCCCAGG TGGAGAGTGA AAGCCAAAAC GTCGACGAGG TGGGAAAACC AGAGGAGCCA 
CAGGCCGAAA TGGAAATAGA AAAAGAAAAA GAAGAAACGG CAGCGACCGA AGCCGCAGAA 
GTGGAGCTGC CGGCCACAGA AGCCAAACCA GATATGGAAT CCGAGATCGA TCAGGTGGAA 
CAAAGCGCCG ACATCAAGCC GACAAGTGGC GAAGATGATT TAAATGAAGC CAATCAATTA 
AATCAAGACC AAGTCACCGA GCCAGCCGCT CTTGAACAGG AGGAGATCGA AGAAAAAGAG 
CCCGAGCAGG TGGTGGATAT GCATGAAGAT GCAGATGAAG CCACAACGGA GAAACCAGTC 
CAAGATCACG ACCAGGAGGA GGTGGAGGCA ATCACCGAAG CGGAGCACGG ACAATTGACA 
TACCCAACAA ATGATGATGA ATTGGCGCCA GTGGAAATAG AACCAGAGCT GACCACAGAG 
GAACCAGAAA TCGCTGAGAA CACCACCGAG CTGCCCGAGC AACAGCAACA GCAGCAGGAT 
GAGGAGGAGA TTGAAGAGGC GGCACTTGCA CATGAGGAGC AGGTGCAGCA GGTGGAGCCC 
GAGGAAAATG CAAATGCAAT TAAGACGCCC GAAGTGTCGG AAGCAATTGC AGAGCCCCAG 
GCGGATCTAG AAACCCCGTC AGCCACCGAG AAGGAGATCG AACCACAGAC AGCGGACCAT 
GTTGAGGAGG AGCCAGTGGC GATAACCGAG CGCATTCAAG AGGCAGAGCC AGAGCCAGAA 
ATTGAGCCCA CTAATGACGA GGTGTCGCAG GTGGATGCGG ACCAGGCCGA GCCAGAGCTG 
CCAATTAAGA GCGATGCCGA CATCGCGGAG GAAGCCGCCG AGGAACCTGC TCAGACGGAG 
GTGGCTCCCG AAGTGGAAAC GGAAACCGAG GCCAGTGAAA GTGCTCAGAC ATCTCCAGAG 
ATTAGCGTAG AGCAAGATGA AGTTGCGATC GATAAAAACG AGAATGACAA CATTATCCAG 
GGAGCCGAAC CCAATGAAGA GGACGAACTT ATCCCGGAAC CACAACCAAT GCCAGAAGAG 
GAGGAATTGC CAGTTCAACA GGAAGCATCA GCTGTTCCGG AGGATCATAA GTTGACCAAG 
CCAGAAATGG ACTATACCAG CGTGGAGCAT CTACAGGAAC TTTCCCGGCC AGAAGAAACG 
ACTGCTGCTA TTGAAGCTGA AGAATCTGAC TCTCGCGAAT CCACTCTGGC TCCAGAGGCT 
CCAGATGCAC CTATTGCCAT TGTGAGCGAG GAACGCGATC CCGAACATGA TCCTCAGAAC 
TTCCATGAGA GCGAGAGCAT TGCTGATATC CTCAGCGACT TGATGTTGGA GGCTGACAGC 
ACTGTGCCTC CTGTTCCATT TGGTCAGCAA CCCGCCCAAA CGGTTCCCAT GGTGGAGGCC 
AACTCGGAAA ACGAGGAGGA ACAAGCACAT GAAGCCATTT CGGATCTCCA GGCTGAAGCC 
GATGAGACCC AGGAACATGC CGAGCAAGTG CAACCGGTTA ACGAGCCAGG AACTGCTATT 
CCCGAACTGT TTGCAGAGGC AGCTAAGGAT GAAGAAGATG TCCCAGCTCT CCCAGAATCT 
GATGAATCGA AAACCGAACA ACCAAGCGCT CTGGAGGTGG AGCAGCACAT TGCCAGTGAA 
GCGGAGCAAA CGCCAGTGGA AGATGAGCAC CAGTATGATG AAGAAGAGCA CCAGCAGGAT 
CAGGAGGAAC ATCAGCAGGA TGAGGACGAG CCCCTTAAGG AAGTAGAAAC CGAATCCCTG 
CCAGAAGAAG CCACCACAGA AAAGTTCCAG GAGCCCGAGC CCGAAGAAGT TAACACTCCG 
TTGCCCGAGG AACTGCCCTT CGATCTTACA GATCACTCCG ATGTGAACCG CTTCAATGAG 
CTGCAATCCG AAAGTCTAAT TCTGGAATCC ACTACCAATA TCGATGGTCT GCAGGAGCTG 
GACAACAACC ATATTGAGGA AGAGGAGCCA GCAGCTCATG CCATTCCACA TGAGGAGGCC 
ACACCCAATC CCGCTGACAT TGTCGAGATC ACCACACAAA CCATGTTGGG CCTGGCCAGT 
CGTGTGACCC TTATGGAGCC CGCTGCTCCC GTCGTTACCA CGCTAATGCC CCTAATGACC 
GCCGATGAAC CCACGCCGGA ACCCGTTTCT CCAGCAGCCA TTCCTTCCGC CTCTCTGGCT 
GCCATCAAAC CCGGTACGGA GTTGCGCAAG CGCGTGGATT TGGGTCTGGA AGCAGTTTCA 
CTTGGATTGG CCTGCAGCAG TGATCGTCAG TGCCAGTTGG CTGATCCCCA CACCGTGTGC 
AATAGACGAG GAGTCTGCGA TTGTGCTGCC GGAGAAGAGG GATCACAGTG CAGCGCCGAG 
AGAACCGGAT GCAGTCCGGG AACCTTCCAG TGCCGCTCGA GTGGCGTGTG CATCTCGTGG 
TTCTTTGTGT GCGACGGCCG CGCCGACTGC AATGACGCCT CCGACGAGGA GTGCACCCAC 
AACGCCCGCC TCAACCAGAC CTGCCCCACG GAATCCTTCC GCTGCCAGCG AAGCGGTCGC 
TGCATCTCGC GCGCTGCGCT GTGCGACGGA CGACGGCAGT GTCCACATGG CGAGGACGAG 
CTGGGCTGCG ATGGCAGCGT GAAGGGCGGC AACGCCTGTC CGGAACACAC CTTCCGCTGC 
GGCAGCGGCG AGTGCCTGCC GGAGTACGAG TACTGTAACG CGATCGTGTC CTGCAAGGAC 
GGCAGCGATG AGCCGCCGCA TCTGTGTGGA TCCCGCGCCC TCCCCAACCT CTTCATGCGC 
CTCATCGAGG CGGGCGGCCT GCTCGGCGGT GGTCGGCGGG AGGCGGACGC CTACTGTCCG 
CACCGCTGCA GCAACGGACT GTGCCGCTCC ACGGCCATCG TCTGCTCCGG ACGGGATGGT 
TGCGGTGATG GCACCGACGA ACAAACCTGC TCCGTGTGCC GCTGTCCCGC CCCGACTGCT 
GCCAGCCTGC CCGCATTCTT GGCCCGACAA CGCCCCATGC CGCTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524849.2
CDS	355..5205
Translation	MTATARQSATSRKQPAIAMLNGNAFTVKRLLALLIIFTVVDASRSLELGDKCQHDMDCTDFIKGSSCSALGYCECAPYFVQLDSKRCLSSQLLGGDCQLSEQCSMKVANSSCLEGACRCVEGFLQFRKHTCLGPAHPGAVCYSHAHCQMFDTRTHCDFLIPNLFGRCQCTSPAKMVGGLCVAPAADQEDQKQEQAVEKVEQPASSTTTTTTTTTTPRPTTRTTSRTTTTTTAAPPTTTRRTTTTTTTTTPAPILLEDELPASVEEQSLEEDDGETTKLRPEVVEVDNGEKLEAVDLAHEETELTQQQNQLDEQQHQEEEQKHQEEEQHRIEQQQNQEEEQQQQQHQQQVIDQASDAELPQSSQDATTGVQQLLTPADVPTEDAINAEQETEAGPTEDYPYKIDEEYTEEHNDKPEAPELTVDQPEHEESPVATEIVPIEADEEAAEASGGQEIASSPIDDTMKFDYETSVDDVEPAKPEDEKETEAVHQATNEGVVPIDTESETDAETSTEPQIQTQLLQEDGEPETSQTVADEELIPVNEGETESVTKAADETEEIPEEETPEDLASGDHELDVAAASHDDEVETPKQEEPTEALKIEQPAQVESESQNVDEVGKPEEPQAEMEIEKEKEETAATEAAEVELPATEAKPDMESEIDQVEQSADIKPTSGEDDLNEANQLNQDQVTEPAALEQEEIEEKEPEQVVDMHEDADEATTEKPVQDHDQEEVEAITEAEHGQLTYPTNDDELAPVEIEPELTTEEPEIAENTTELPEQQQQQQDEEEIEEAALAHEEQVQQVEPEENANAIKTPEVSEAIAEPQADLETPSATEKEIEPQTADHVEEEPVAITERIQEAEPEPEIEPTNDEVSQVDADQAEPELPIKSDADIAEEAAEEPAQTEVAPEVETETEASESAQTSPEISVEQDEVAIDKNENDNIIQGAEPNEEDELIPEPQPMPEEEELPVQQEASAVPEDHKLTKPEMDYTSVEHLQELSRPEETTAAIEAEESDSRESTLAPEAPDAPIAIVSEERDPEHDPQNFHESESIADILSDLMLEADSTVPPVPFGQQPAQTVPMVEANSENEEEQAHEAISDLQAEADETQEHAEQVQPVNEPGTAIPELFAEAAKDEEDVPALPESDESKTEQPSALEVEQHIASEAEQTPVEDEHQYDEEEHQQDQEEHQQDEDEPLKEVETESLPEEATTEKFQEPEPEEVNTPLPEELPFDLTDHSDVNRFNELQSESLILESTTNIDGLQELDNNHIEEEEPAAHAIPHEEATPNPADIVEITTQTMLGLASRVTLMEPAAPVVTTLMPLMTADEPTPEPVSPAAIPSASLAAIKPGTELRKRVDLGLEAVSLGLACSSDRQCQLADPHTVCNRRGVCDCAAGEEGSQCSAERTGCSPGTFQCRSSGVCISWFFVCDGRADCNDASDEECTHNARLNQTCPTESFRCQRSGRCISRAALCDGRRQCPHGEDELGCDGSVKGGNACPEHTFRCGSGECLPEYEYCNAIVSCKDGSDEPPHLCGSRALPNLFMRLIEAGGLLGGGRREADAYCPHRCSNGLCRSTAIVCSGRDGCGDGTDEQTCSVCRCPAPTAASLPAFLARQRPMPLW

Target ORF information:

RefSeq Version	NM_080110.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-inducible gene E1 (ImpE1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080110.3

ORF Insert Sequence:

ATGACGGCGA CTGCGAGGCA GTCGGCAACA TCGAGAAAGC AACCTGCGAT CGCAATGCTC 
AATGGAAACG CTTTTACGGT TAAGCGGCTC TTAGCGCTGC TGATCATCTT CACAGTGGTG 
GACGCGAGTC GATCCCTGGA GCTGGGCGAC AAGTGCCAGC ATGACATGGA CTGCACGGAT 
TTCATCAAGG GCAGCAGCTG CTCCGCCCTC GGATACTGCG AGTGCGCCCC CTACTTCGTC 
CAGTTGGATT CCAAGCGCTG TTTGTCATCC CAGCTCCTTG GCGGCGACTG CCAGCTGAGC 
GAGCAGTGCT CCATGAAGGT GGCCAATAGC AGTTGTCTGG AGGGTGCATG CCGCTGCGTC 
GAGGGATTCC TGCAGTTCCG CAAGCACACC TGTCTGGGAC CTGCCCATCC TGGTGCCGTT 
TGCTACAGCC ACGCCCACTG TCAAATGTTC GACACCCGCA CCCACTGTGA CTTCCTCATC 
CCGAATCTCT TCGGACGCTG CCAGTGCACA AGTCCAGCCA AAATGGTGGG TGGTCTGTGT 
GTGGCCCCGG CGGCGGATCA GGAGGATCAG AAGCAGGAAC AGGCCGTGGA GAAGGTAGAG 
CAGCCAGCTA GCAGTACCAC TACCACGACG ACAACAACAA CCACTCCTAG GCCCACAACT 
AGAACCACGA GTAGAACAAC GACGACCACC ACTGCAGCAC CACCAACAAC CACCAGAAGA 
ACAACAACGA CCACAACTAC GACAACTCCA GCGCCCATTC TCCTAGAAGA TGAGCTGCCC 
GCCAGCGTGG AGGAGCAATC GCTGGAGGAA GACGATGGTG AGACCACCAA GCTGAGACCC 
GAGGTCGTTG AGGTTGATAA TGGTGAAAAA TTGGAGGCTG TGGACCTGGC TCATGAGGAA 
ACCGAACTGA CACAGCAGCA AAATCAATTA GATGAGCAGC AACATCAGGA GGAGGAACAG 
AAACATCAAG AGGAGGAACA ACATAGGATT GAGCAGCAGC AGAACCAAGA GGAGGAGCAG 
CAGCAGCAGC AGCATCAGCA GCAGGTCATA GACCAAGCAA GCGACGCAGA GCTTCCTCAA 
TCCTCCCAAG ATGCCACCAC TGGAGTCCAA CAGCTGCTCA CACCTGCTGA TGTGCCAACC 
GAGGATGCCA TTAATGCGGA ACAGGAAACT GAGGCTGGAC CCACTGAAGA TTACCCATAC 
AAGATCGATG AGGAGTACAC AGAGGAGCAC AATGACAAGC CAGAAGCTCC AGAACTGACA 
GTGGATCAAC CTGAGCACGA AGAATCCCCA GTAGCCACTG AAATCGTGCC TATCGAAGCG 
GATGAGGAGG CTGCTGAGGC CTCGGGTGGT CAAGAGATTG CCTCATCGCC CATCGACGAT 
ACCATGAAAT TCGATTATGA AACATCAGTG GATGATGTTG AGCCAGCCAA GCCAGAAGAT 
GAAAAGGAGA CGGAGGCAGT ACACCAAGCG ACTAATGAGG GGGTGGTGCC CATTGATACC 
GAAAGCGAGA CTGATGCAGA GACTAGCACC GAGCCCCAGA TCCAGACGCA GCTGCTGCAG 
GAGGATGGAG AGCCAGAGAC ATCGCAGACT GTGGCCGATG AAGAACTGAT CCCGGTTAAT 
GAAGGTGAAA CCGAAAGTGT GACGAAGGCA GCCGACGAGA CCGAGGAGAT ACCGGAGGAG 
GAAACACCTG AGGATCTGGC TTCGGGAGAT CATGAGCTGG ATGTTGCTGC AGCCAGCCAC 
GATGATGAGG TGGAGACGCC CAAGCAGGAG GAGCCAACAG AAGCGTTGAA GATCGAACAG 
CCAGCCCAGG TGGAGAGTGA AAGCCAAAAC GTCGACGAGG TGGGAAAACC AGAGGAGCCA 
CAGGCCGAAA TGGAAATAGA AAAAGAAAAA GAAGAAACGG CAGCGACCGA AGCCGCAGAA 
GTGGAGCTGC CGGCCACAGA AGCCAAACCA GATATGGAAT CCGAGATCGA TCAGGTGGAA 
CAAAGCGCCG ACATCAAGCC GACAAGTGGC GAAGATGATT TAAATGAAGC CAATCAATTA 
AATCAAGACC AAGTCACCGA GCCAGCCGCT CTTGAACAGG AGGAGATCGA AGAAAAAGAG 
CCCGAGCAGG TGGTGGATAT GCATGAAGAT GCAGATGAAG CCACAACGGA GAAACCAGTC 
CAAGATCACG ACCAGGAGGA GGTGGAGGCA ATCACCGAAG CGGAGCACGG ACAATTGACA 
TACCCAACAA ATGATGATGA ATTGGCGCCA GTGGAAATAG AACCAGAGCT GACCACAGAG 
GAACCAGAAA TCGCTGAGAA CACCACCGAG CTGCCCGAGC AACAGCAACA GCAGCAGGAT 
GAGGAGGAGA TTGAAGAGGC GGCACTTGCA CATGAGGAGC AGGTGCAGCA GGTGGAGCCC 
GAGGAAAATG CAAATGCAAT TAAGACGCCC GAAGTGTCGG AAGCAATTGC AGAGCCCCAG 
GCGGATCTAG AAACCCCGTC AGCCACCGAG AAGGAGATCG AACCACAGAC AGCGGACCAT 
GTTGAGGAGG AGCCAGTGGC GATAACCGAG CGCATTCAAG AGGCAGAGCC AGAGCCAGAA 
ATTGAGCCCA CTAATGACGA GGTGTCGCAG GTGGATGCGG ACCAGGCCGA GCCAGAGCTG 
CCAATTAAGA GCGATGCCGA CATCGCGGAG GAAGCCGCCG AGGAACCTGC TCAGACGGAG 
GTGGCTCCCG AAGTGGAAAC GGAAACCGAG GCCAGTGAAA GTGCTCAGAC ATCTCCAGAG 
ATTAGCGTAG AGCAAGATGA AGTTGCGATC GATAAAAACG AGAATGACAA CATTATCCAG 
GGAGCCGAAC CCAATGAAGA GGACGAACTT ATCCCGGAAC CACAACCAAT GCCAGAAGAG 
GAGGAATTGC CAGTTCAACA GGAAGCATCA GCTGTTCCGG AGGATCATAA GTTGACCAAG 
CCAGAAATGG ACTATACCAG CGTGGAGCAT CTACAGGAAC TTTCCCGGCC AGAAGAAACG 
ACTGCTGCTA TTGAAGCTGA AGAATCTGAC TCTCGCGAAT CCACTCTGGC TCCAGAGGCT 
CCAGATGCAC CTATTGCCAT TGTGAGCGAG GAACGCGATC CCGAACATGA TCCTCAGAAC 
TTCCATGAGA GCGAGAGCAT TGCTGATATC CTCAGCGACT TGATGTTGGA GGCTGACAGC 
ACTGTGCCTC CTGTTCCATT TGGTCAGCAA CCCGCCCAAA CGGTTCCCAT GGTGGAGGCC 
AACTCGGAAA ACGAGGAGGA ACAAGCACAT GAAGCCATTT CGGATCTCCA GGCTGAAGCC 
GATGAGACCC AGGAACATGC CGAGCAAGTG CAACCGGTTA ACGAGCCAGG AACTGCTATT 
CCCGAACTGT TTGCAGAGGC AGCTAAGGAT GAAGAAGATG TCCCAGCTCT CCCAGAATCT 
GATGAATCGA AAACCGAACA ACCAAGCGCT CTGGAGGTGG AGCAGCACAT TGCCAGTGAA 
GCGGAGCAAA CGCCAGTGGA AGATGAGCAC CAGTATGATG AAGAAGAGCA CCAGCAGGAT 
CAGGAGGAAC ATCAGCAGGA TGAGGACGAG CCCCTTAAGG AAGTAGAAAC CGAATCCCTG 
CCAGAAGAAG CCACCACAGA AAAGTTCCAG GAGCCCGAGC CCGAAGAAGT TAACACTCCG 
TTGCCCGAGG AACTGCCCTT CGATCTTACA GATCACTCCG ATGTGAACCG CTTCAATGAG 
CTGCAATCCG AAAGTCTAAT TCTGGAATCC ACTACCAATA TCGATGGTCT GCAGGAGCTG 
GACAACAACC ATATTGAGGA AGAGGAGCCA GCAGCTCATG CCATTCCACA TGAGGAGGCC 
ACACCCAATC CCGCTGACAT TGTCGAGATC ACCACACAAA CCATGTTGGG CCTGGCCAGT 
CGTGTGACCC TTATGGAGCC CGCTGCTCCC GTCGTTACCA CGCTAATGCC CCTAATGACC 
GCCGATGAAC CCACGCCGGA ACCCGTTTCT CCAGCAGCCA TTCCTTCCGC CTCTCTGGCT 
GCCATCAAAC CCGGTACGGA GTTGCGCAAG CGCGTGGATT TGGGTCTGGA AGCAGTTTCA 
CTTGGATTGG CCTGCAGCAG TGATCGTCAG TGCCAGTTGG CTGATCCCCA CACCGTGTGC 
AATAGACGAG GAGTCTGCGA TTGTGCTGCC GGAGAAGAGG GATCACAGTG CAGCGCCGAG 
AGAACCGGAT GCAGTCCGGG AACCTTCCAG TGCCGCTCGA GTGGCGTGTG CATCTCGTGG 
TTCTTTGTGT GCGACGGCCG CGCCGACTGC AATGACGCCT CCGACGAGGA GTGCACCCAC 
AACGCCCGCC TCAACCAGAC CTGCCCCACG GAATCCTTCC GCTGCCAGCG AAGCGGTCGC 
TGCATCTCGC GCGCTGCGCT GTGCGACGGA CGACGGCAGT GTCCACATGG CGAGGACGAG 
CTGGGCTGCG ATGGCAGCGT GAAGGGCGGC AACGCCTGTC CGGAACACAC CTTCCGCTGC 
GGCAGCGGCG AGTGCCTGCC GGAGTACGAG TACTGTAACG CGATCGTGTC CTGCAAGGAC 
GGCAGCGATG AGCCGCCGCA TCTGTGTGGA TCCCGCGCCC TCCCCAACCT CTTCATGCGC 
CTCATCGAGG CGGGCGGCCT GCTCGGCGGT GGTCGGCGGG AGGCGGACGC CTACTGTCCG 
CACCGCTGCA GCAACGGACT GTGCCGCTCC ACGGCCATCG TCTGCTCCGG ACGGGATGGT 
TGCGGTGATG GCACCGACGA ACAAACCTGC TCCGTGTGCC GCTGTCCCGC CCCGACTGCT 
GCCAGCCTGC CCGCATTCTT GGCCCGACAA CGCCCCATGC CGCTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ImpE1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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