e(y)2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	e(y)2
Entrez Gene ID	45848
Full Name	enhancer of yellow 2
Synonyms	CG15191,Dmel\CG15191,E(y)2,ENY2,Sus1,d,e(y)[2]
General protein information	Preferred Names enhancer of yellow 2 Names CG15191 gene product from transcript CG15191-RA CG15191-PA e(y)2-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10C7-10C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_080107.3	NP_524846.1	enhancer of yellow 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Evolutionarily conserved ENY2 is responsible for barrier activity mediated by the dCTCF protein.
Title: Highly conserved ENY2/Sus1 protein binds to Drosophila CTCF and is required for barrier activity.

The transcription coactivators SAYP and ENY2 act in gene expression as cooperative nanoregulators,determining the molecular mechanisms of spatial and temporal coordination of the processes involved in the realization of genetic information.
Title: [Functioning of macromolecular complexes at successive stages of gene expression as self-coordinated molecular machines].

ENY2 protein forms a part of the THO complex of Drosophila melanogaster.
Title: ENY2 protein forms a part of the THO complex of Drosophila melanogaster.

368-bp core of E(y)2 insulator is sufficient for blocking Polycomb response element-mediated silencing.
Title: E(y)2/Sus1 is required for blocking PRE silencing by the Wari insulator in Drosophila melanogaster.

ENY2 in Drosophila is also stably associated with THO, the complex involved in mRNP biogenesis
Title: Multifunctional factor ENY2 is associated with the THO complex and promotes its recruitment onto nascent mRNA.

The following e(y)2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the e(y)2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02391	NM_080107.3 Latest version!	Drosophila melanogaster enhancer of yellow 2 (e(y)2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

e(y)2 ( NM_080107.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524846.1 Drosophila melanogaster enhancer of yellow 2 (e(y)2), mRNA.

Price & Availability↑

CloneID	OFa02391
Clone ID Related Accession (Same CDS sequence)	NM_080107.3
Accession Version	NM_080107.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of yellow 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_080107.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACTT CCGGCGCAGT TGATCAGTAC ACGGTGCTAA CCGGCGACCG TTCCAAGATC 
AAGGATTTGC TCTGCAGCCG CCTTACGGAA TGTGGCTGGC GGGACGAGGT GCGTCTGATG 
TGCCGCAACA TCCTGATGGA GAAGGGCACC AACAACAGCT TCACCGTAGA GCAGCTGATC 
GCCGAGGTAA CACCCAAGGC ACGCACCCTG GTCCCGGATG CCGTCAAAAA GGAGCTGCTT 
ATGAAGATAC GCACCATTCT CACGGAAATT GAGGAGGAAC CCGATGAGCC AGAGGACGAA 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524846.1
CDS	143..448
Translation	MSTSGAVDQYTVLTGDRSKIKDLLCSRLTECGWRDEVRLMCRNILMEKGTNNSFTVEQLIAEVTPKARTLVPDAVKKELLMKIRTILTEIEEEPDEPEDES

Target ORF information:

RefSeq Version	NM_080107.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of yellow 2 (e(y)2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080107.3

ORF Insert Sequence:

ATGAGCACTT CCGGCGCAGT TGATCAGTAC ACGGTGCTAA CCGGCGACCG TTCCAAGATC 
AAGGATTTGC TCTGCAGCCG CCTTACGGAA TGTGGCTGGC GGGACGAGGT GCGTCTGATG 
TGCCGCAACA TCCTGATGGA GAAGGGCACC AACAACAGCT TCACCGTAGA GCAGCTGATC 
GCCGAGGTAA CACCCAAGGC ACGCACCCTG GTCCCGGATG CCGTCAAAAA GGAGCTGCTT 
ATGAAGATAC GCACCATTCT CACGGAAATT GAGGAGGAAC CCGATGAGCC AGAGGACGAA 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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e(y)2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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