onecut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	onecut
Entrez Gene ID	45816
Full Name	CG1922 gene product from transcript CG1922-RB
Synonyms	A1,CG1922,Dmel\CG1922
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102D1-102D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272136.1	NP_001259065.1	onecut, isoform B
NM_080103.3	NP_524842.2	onecut, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following onecut gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the onecut cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00209	NM_080103.3 Latest version!	Drosophila melanogaster onecut, transcript variant A (onecut), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00209	NM_001272136.1 Latest version!	Drosophila melanogaster onecut, transcript variant B (onecut), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

onecut ( NM_001272136.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259065.1 Drosophila melanogaster onecut, transcript variant B (onecut), mRNA.

Price & Availability↑

CloneID	OFa00209
Clone ID Related Accession (Same CDS sequence)	NM_001272136.1 , NM_080103.3
Accession Version	NM_001272136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	onecut, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCCT TAAATGATAT AATTGACCAC CAAACATTTA GCCAGGAGTT AGTTGAAGAT 
GCATCGGAGT TCATCACTGT GGGTCATCAT TCGGAGCGCC CTTCGCAGTC CAGTCAGCAG 
CCTAATTCTG GACAGGATCT GACAATGTCT ATGCAAGACA TAATTTCCTG TCCGGTGAAA 
CACCGGACCT GCTCTGCATC TGGGTCGGGA TCGGCATCGG GATCCGATTC CGTTGTCATG 
GTTATAGACG CACTGGGACA AGGTAACAGG CAGTCAGCAT ATCAAATTGT GCCTCAGCAG 
TTGCAGCAAA GAAACATGCC ATTGCCTTTC GGGCTACTTG AACGAGATAG GCAGCATATG 
CAGCATGGGC GTGAGGTCAA TACCAGTCCA GTGGACTTTG TATCATCAGA TATAAATTTG 
GATGGTCTGA CTGTAGACGC AGACGTCTCC CAGACGGATC ACTCTCAAGA AACGGCGGTA 
AAGCAGGAGC AAAAACTACT TATTGTGCAA TCCAAGAGTC AGGATCAAAG CCATAGGCGC 
ATTCGAATGC TTGTAGACGT TTCGAGCGTA AATTCTGGCC TTGGCGTGCA CGTGGATGAT 
ATGGATGAGA TTTCCTCTGA TGGCGTTGGC TGCGACGACG AAGGAGTTAC GCTGAGCCAC 
CAGCATCTTT TGGAGCAAGA GGAACAATTC GGCCTAACAA GCCACCATCC GCATCTCCAA 
CCTCATACAC AGATCATCCA TGGCTTGCAC CAGAGATCAA CACATTCGGA AATGGGACTG 
GACAATGGGC ATGGGGAGGT TCTATCGGTT ATCGTCCATT CCCAGGACAG CGACAAGGAG 
GACTGTGAGG AAAATGATGA TGGGGATGCA GAAGGAGATT TAGAAAATGA AGACGATGAT 
GAACGCGACT CGCGGAGTCG TGAACAGCTG CTTAGTCACA GCTCGTATCA GACGCTGACC 
TCGGTGAATG ATCGCCTATC TTCGCCTGGG TTTAGTCAAA CATCGTATGC TACACTCACC 
CCTATACAAC CACTTCCGCC TATATCAACG ATGTCCGAGA AGTTCGCCTA CTCTGGCCAC 
ATCTCGGGAG GAGACAGTGG AGACACGGAT GTCAATGGAG ATGGTGCAGG TGGAGGAGTC 
GTTGAGGTTG GTGAAGTCAC CAACCAATCC AGCGAGGCTA CAGGGACTGT TTCAATATCT 
AGTGGCAATG CTACATCCTC TGTGTGCTCC AACAACGATT GCAGTTCTTT TTCTGCCCTT 
TCCATGCCCA TAGGAAGTGG CCATTTGGGT CTAGGTGTTT TAAGCGGCGT CCAGTCACCA 
TTTTCTTCAT ACGAAAAGCT ATCTTCGATG ATTTCCCCTC CGCCTAATAA CTATTTGGTC 
TCGTGCGATC TACATTCCTC CGTTTCGGGA CGCGTTATAA ATTCATCGCA CTTGCAGCTA 
AGTCACAACG GCAATAAAAA GGAGTCTGGA ACACACGAAC ACACACATAG GCCTGCTGAT 
GTCAATGGTG GGAAGTTTTC CTATACTGGC CACATCTCCC GAGGTGATAG TGTAGATAAT 
GACGTCAACG GTGAAAAGTT CTCTTTCTCT GACCATATCT CCGGTGGGGA TAGCGGAGAT 
GAAGATGCCA ACAGAGAAAA ATTCATATAC TCAGATCACA TCTCCGAAGG AGAAAACGGA 
CCGGACGTCA ATAGCGGAAC GAATTGGCTC CAGATGCACT CTGAGAGGGA AGTGCGGCTC 
CATATGCCCG TGCCGGCTGA GCTGGAAGCG AGGTTTCACA TTTCGTCAGA GCGAAGGACT 
CGACTCAATG TGCCACCTGC TCGTGGTTCT CTAAGCCGAC ATCTTGCTCC AAACGCTCCG 
ATCTGCCCCG CAGATTGGAA AGCGGATGAT TGGAAACACA GCAATGCTGG CGTTGTTAGT 
TTGACCGCAG ATATGCCCGT GGTTGTGTCT CTTACTCCTA CACCGCCTCC GCTAAGAGAC 
GACTCGGTGA GCGGCATTAA ACAAAACGAG CGATCATCAC CAGGGCACAG AGAACAGTAC 
TTCCTGGACA AAAGCCAAGA ACCCAGTTCC GTTGTAGCAG ATCAGTGCAG TCCGGGCATC 
AATGGCACCC CGCAGTCGGT GTGTGTCATT CACCAGCAGT CTCCATCAGC TCTCGGAAAC 
GGGTTTCAAT CAAGCAGCCA GCAGGCCAAG GTTTCCTCGT GCGCATCACC AAAAGGGACA 
GTGTCCTCTG GAGGTGCAGT TTCCAACCGC ATTGCAAATT CCAGTGACAT GGAGGAGATC 
AATACTAAGG ATCTGGCACA GCGCATCTCA GCCGAACTCA AGCGATACAG CATTCCGCAG 
GCTATATTCG CACAACGGGT ACTATGCCGA TCGCAAGGCA CCTTGTCCGA TCTGCTGCGA 
AACCCAAAGC CGTGGTCAAA GCTGAAATCC GGCCGAGAGA CGTTTCGCAG AATGTATAAA 
TGGCTCCAGG AGCCCGAGTT TCAGCGCATG TCTGCTCTGC GAATGGCCGC TGCCCAAATT 
CCTCAGCGAG CTCCTTTGAG CTCTGGAATG TCTCTTGGGT CAGCAACAGG TCCAAGTGGC 
TCCACCGGCA CAATACCGAC GGACTTGGAC CCACATGGCG GCCCTACGAT GATCCAAAAT 
CCTCTGACAA ACGAATCGGA CTCTTCGCCT GCATCAACAC CTGTCACAAG CGTTTTGGTT 
GGGAGCGTCG TTAGCTGTCG TCGAAAGGAA GAGCCCCAGA TCGAACAAAT GCCCCAGCCT 
AAAAAGCCGC GATTGGTCTT CACCGATCTC CAGCGACGAA CATTACAAGC TATTTTCAAA 
GAAACTAAAA GACCTTCGAA GGAGATGCAA GTGACCATCG CGAGACAGCT GGGACTGGAG 
CCTACTACAG TGGGAAATTT TTTTATGAAC GCCAGAAGAA GGTCGATGGA TAAGTGGCGA 
GATGATGACT CCAAAAGCAC TATGCATGTG GCACATAGCC GACAACAACA AGACGAACAG 
CAGAAAGATA ATGCCCACAC TCATAGTCAA TCTCAAATTC AAGATCAAAA TCACTCTCAA 
AATCAAGCTA TCCATAATAG CATGTCGCAA GATCCCTACT CAAATCTTCA CACAACAGCT 
ATGTCGCCTC TTGGAGCTTT TGATGAAGAC GCTGATATGG ACTTGGAACT AGAAAGTCAC 
GACTTTGATT TAGTGGACCC CGATGAACAT GGGGATACCA ATGATCCAAA CGGCGACATG 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259065.1
CDS	256..3501
Translation	MDSLNDIIDHQTFSQELVEDASEFITVGHHSERPSQSSQQPNSGQDLTMSMQDIISCPVKHRTCSASGSGSASGSDSVVMVIDALGQGNRQSAYQIVPQQLQQRNMPLPFGLLERDRQHMQHGREVNTSPVDFVSSDINLDGLTVDADVSQTDHSQETAVKQEQKLLIVQSKSQDQSHRRIRMLVDVSSVNSGLGVHVDDMDEISSDGVGCDDEGVTLSHQHLLEQEEQFGLTSHHPHLQPHTQIIHGLHQRSTHSEMGLDNGHGEVLSVIVHSQDSDKEDCEENDDGDAEGDLENEDDDERDSRSREQLLSHSSYQTLTSVNDRLSSPGFSQTSYATLTPIQPLPPISTMSEKFAYSGHISGGDSGDTDVNGDGAGGGVVEVGEVTNQSSEATGTVSISSGNATSSVCSNNDCSSFSALSMPIGSGHLGLGVLSGVQSPFSSYEKLSSMISPPPNNYLVSCDLHSSVSGRVINSSHLQLSHNGNKKESGTHEHTHRPADVNGGKFSYTGHISRGDSVDNDVNGEKFSFSDHISGGDSGDEDANREKFIYSDHISEGENGPDVNSGTNWLQMHSEREVRLHMPVPAELEARFHISSERRTRLNVPPARGSLSRHLAPNAPICPADWKADDWKHSNAGVVSLTADMPVVVSLTPTPPPLRDDSVSGIKQNERSSPGHREQYFLDKSQEPSSVVADQCSPGINGTPQSVCVIHQQSPSALGNGFQSSSQQAKVSSCASPKGTVSSGGAVSNRIANSSDMEEINTKDLAQRISAELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMYKWLQEPEFQRMSALRMAAAQIPQRAPLSSGMSLGSATGPSGSTGTIPTDLDPHGGPTMIQNPLTNESDSSPASTPVTSVLVGSVVSCRRKEEPQIEQMPQPKKPRLVFTDLQRRTLQAIFKETKRPSKEMQVTIARQLGLEPTTVGNFFMNARRRSMDKWRDDDSKSTMHVAHSRQQQDEQQKDNAHTHSQSQIQDQNHSQNQAIHNSMSQDPYSNLHTTAMSPLGAFDEDADMDLELESHDFDLVDPDEHGDTNDPNGDML

Target ORF information:

RefSeq Version	NM_001272136.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster onecut, transcript variant B (onecut), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272136.1

ORF Insert Sequence:

ATGGATTCCT TAAATGATAT AATTGACCAC CAAACATTTA GCCAGGAGTT AGTTGAAGAT 
GCATCGGAGT TCATCACTGT GGGTCATCAT TCGGAGCGCC CTTCGCAGTC CAGTCAGCAG 
CCTAATTCTG GACAGGATCT GACAATGTCT ATGCAAGACA TAATTTCCTG TCCGGTGAAA 
CACCGGACCT GCTCTGCATC TGGGTCGGGA TCGGCATCGG GATCCGATTC CGTTGTCATG 
GTTATAGACG CACTGGGACA AGGTAACAGG CAGTCAGCAT ATCAAATTGT GCCTCAGCAG 
TTGCAGCAAA GAAACATGCC ATTGCCTTTC GGGCTACTTG AACGAGATAG GCAGCATATG 
CAGCATGGGC GTGAGGTCAA TACCAGTCCA GTGGACTTTG TATCATCAGA TATAAATTTG 
GATGGTCTGA CTGTAGACGC AGACGTCTCC CAGACGGATC ACTCTCAAGA AACGGCGGTA 
AAGCAGGAGC AAAAACTACT TATTGTGCAA TCCAAGAGTC AGGATCAAAG CCATAGGCGC 
ATTCGAATGC TTGTAGACGT TTCGAGCGTA AATTCTGGCC TTGGCGTGCA CGTGGATGAT 
ATGGATGAGA TTTCCTCTGA TGGCGTTGGC TGCGACGACG AAGGAGTTAC GCTGAGCCAC 
CAGCATCTTT TGGAGCAAGA GGAACAATTC GGCCTAACAA GCCACCATCC GCATCTCCAA 
CCTCATACAC AGATCATCCA TGGCTTGCAC CAGAGATCAA CACATTCGGA AATGGGACTG 
GACAATGGGC ATGGGGAGGT TCTATCGGTT ATCGTCCATT CCCAGGACAG CGACAAGGAG 
GACTGTGAGG AAAATGATGA TGGGGATGCA GAAGGAGATT TAGAAAATGA AGACGATGAT 
GAACGCGACT CGCGGAGTCG TGAACAGCTG CTTAGTCACA GCTCGTATCA GACGCTGACC 
TCGGTGAATG ATCGCCTATC TTCGCCTGGG TTTAGTCAAA CATCGTATGC TACACTCACC 
CCTATACAAC CACTTCCGCC TATATCAACG ATGTCCGAGA AGTTCGCCTA CTCTGGCCAC 
ATCTCGGGAG GAGACAGTGG AGACACGGAT GTCAATGGAG ATGGTGCAGG TGGAGGAGTC 
GTTGAGGTTG GTGAAGTCAC CAACCAATCC AGCGAGGCTA CAGGGACTGT TTCAATATCT 
AGTGGCAATG CTACATCCTC TGTGTGCTCC AACAACGATT GCAGTTCTTT TTCTGCCCTT 
TCCATGCCCA TAGGAAGTGG CCATTTGGGT CTAGGTGTTT TAAGCGGCGT CCAGTCACCA 
TTTTCTTCAT ACGAAAAGCT ATCTTCGATG ATTTCCCCTC CGCCTAATAA CTATTTGGTC 
TCGTGCGATC TACATTCCTC CGTTTCGGGA CGCGTTATAA ATTCATCGCA CTTGCAGCTA 
AGTCACAACG GCAATAAAAA GGAGTCTGGA ACACACGAAC ACACACATAG GCCTGCTGAT 
GTCAATGGTG GGAAGTTTTC CTATACTGGC CACATCTCCC GAGGTGATAG TGTAGATAAT 
GACGTCAACG GTGAAAAGTT CTCTTTCTCT GACCATATCT CCGGTGGGGA TAGCGGAGAT 
GAAGATGCCA ACAGAGAAAA ATTCATATAC TCAGATCACA TCTCCGAAGG AGAAAACGGA 
CCGGACGTCA ATAGCGGAAC GAATTGGCTC CAGATGCACT CTGAGAGGGA AGTGCGGCTC 
CATATGCCCG TGCCGGCTGA GCTGGAAGCG AGGTTTCACA TTTCGTCAGA GCGAAGGACT 
CGACTCAATG TGCCACCTGC TCGTGGTTCT CTAAGCCGAC ATCTTGCTCC AAACGCTCCG 
ATCTGCCCCG CAGATTGGAA AGCGGATGAT TGGAAACACA GCAATGCTGG CGTTGTTAGT 
TTGACCGCAG ATATGCCCGT GGTTGTGTCT CTTACTCCTA CACCGCCTCC GCTAAGAGAC 
GACTCGGTGA GCGGCATTAA ACAAAACGAG CGATCATCAC CAGGGCACAG AGAACAGTAC 
TTCCTGGACA AAAGCCAAGA ACCCAGTTCC GTTGTAGCAG ATCAGTGCAG TCCGGGCATC 
AATGGCACCC CGCAGTCGGT GTGTGTCATT CACCAGCAGT CTCCATCAGC TCTCGGAAAC 
GGGTTTCAAT CAAGCAGCCA GCAGGCCAAG GTTTCCTCGT GCGCATCACC AAAAGGGACA 
GTGTCCTCTG GAGGTGCAGT TTCCAACCGC ATTGCAAATT CCAGTGACAT GGAGGAGATC 
AATACTAAGG ATCTGGCACA GCGCATCTCA GCCGAACTCA AGCGATACAG CATTCCGCAG 
GCTATATTCG CACAACGGGT ACTATGCCGA TCGCAAGGCA CCTTGTCCGA TCTGCTGCGA 
AACCCAAAGC CGTGGTCAAA GCTGAAATCC GGCCGAGAGA CGTTTCGCAG AATGTATAAA 
TGGCTCCAGG AGCCCGAGTT TCAGCGCATG TCTGCTCTGC GAATGGCCGC TGCCCAAATT 
CCTCAGCGAG CTCCTTTGAG CTCTGGAATG TCTCTTGGGT CAGCAACAGG TCCAAGTGGC 
TCCACCGGCA CAATACCGAC GGACTTGGAC CCACATGGCG GCCCTACGAT GATCCAAAAT 
CCTCTGACAA ACGAATCGGA CTCTTCGCCT GCATCAACAC CTGTCACAAG CGTTTTGGTT 
GGGAGCGTCG TTAGCTGTCG TCGAAAGGAA GAGCCCCAGA TCGAACAAAT GCCCCAGCCT 
AAAAAGCCGC GATTGGTCTT CACCGATCTC CAGCGACGAA CATTACAAGC TATTTTCAAA 
GAAACTAAAA GACCTTCGAA GGAGATGCAA GTGACCATCG CGAGACAGCT GGGACTGGAG 
CCTACTACAG TGGGAAATTT TTTTATGAAC GCCAGAAGAA GGTCGATGGA TAAGTGGCGA 
GATGATGACT CCAAAAGCAC TATGCATGTG GCACATAGCC GACAACAACA AGACGAACAG 
CAGAAAGATA ATGCCCACAC TCATAGTCAA TCTCAAATTC AAGATCAAAA TCACTCTCAA 
AATCAAGCTA TCCATAATAG CATGTCGCAA GATCCCTACT CAAATCTTCA CACAACAGCT 
ATGTCGCCTC TTGGAGCTTT TGATGAAGAC GCTGATATGG ACTTGGAACT AGAAAGTCAC 
GACTTTGATT TAGTGGACCC CGATGAACAT GGGGATACCA ATGATCCAAA CGGCGACATG 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

onecut ( NM_080103.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524842.2 Drosophila melanogaster onecut, transcript variant A (onecut), mRNA.

Price & Availability↑

CloneID	OFa00209
Clone ID Related Accession (Same CDS sequence)	NM_001272136.1 , NM_080103.3
Accession Version	NM_080103.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	onecut, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_080103.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTCCT TAAATGATAT AATTGACCAC CAAACATTTA GCCAGGAGTT AGTTGAAGAT 
GCATCGGAGT TCATCACTGT GGGTCATCAT TCGGAGCGCC CTTCGCAGTC CAGTCAGCAG 
CCTAATTCTG GACAGGATCT GACAATGTCT ATGCAAGACA TAATTTCCTG TCCGGTGAAA 
CACCGGACCT GCTCTGCATC TGGGTCGGGA TCGGCATCGG GATCCGATTC CGTTGTCATG 
GTTATAGACG CACTGGGACA AGGTAACAGG CAGTCAGCAT ATCAAATTGT GCCTCAGCAG 
TTGCAGCAAA GAAACATGCC ATTGCCTTTC GGGCTACTTG AACGAGATAG GCAGCATATG 
CAGCATGGGC GTGAGGTCAA TACCAGTCCA GTGGACTTTG TATCATCAGA TATAAATTTG 
GATGGTCTGA CTGTAGACGC AGACGTCTCC CAGACGGATC ACTCTCAAGA AACGGCGGTA 
AAGCAGGAGC AAAAACTACT TATTGTGCAA TCCAAGAGTC AGGATCAAAG CCATAGGCGC 
ATTCGAATGC TTGTAGACGT TTCGAGCGTA AATTCTGGCC TTGGCGTGCA CGTGGATGAT 
ATGGATGAGA TTTCCTCTGA TGGCGTTGGC TGCGACGACG AAGGAGTTAC GCTGAGCCAC 
CAGCATCTTT TGGAGCAAGA GGAACAATTC GGCCTAACAA GCCACCATCC GCATCTCCAA 
CCTCATACAC AGATCATCCA TGGCTTGCAC CAGAGATCAA CACATTCGGA AATGGGACTG 
GACAATGGGC ATGGGGAGGT TCTATCGGTT ATCGTCCATT CCCAGGACAG CGACAAGGAG 
GACTGTGAGG AAAATGATGA TGGGGATGCA GAAGGAGATT TAGAAAATGA AGACGATGAT 
GAACGCGACT CGCGGAGTCG TGAACAGCTG CTTAGTCACA GCTCGTATCA GACGCTGACC 
TCGGTGAATG ATCGCCTATC TTCGCCTGGG TTTAGTCAAA CATCGTATGC TACACTCACC 
CCTATACAAC CACTTCCGCC TATATCAACG ATGTCCGAGA AGTTCGCCTA CTCTGGCCAC 
ATCTCGGGAG GAGACAGTGG AGACACGGAT GTCAATGGAG ATGGTGCAGG TGGAGGAGTC 
GTTGAGGTTG GTGAAGTCAC CAACCAATCC AGCGAGGCTA CAGGGACTGT TTCAATATCT 
AGTGGCAATG CTACATCCTC TGTGTGCTCC AACAACGATT GCAGTTCTTT TTCTGCCCTT 
TCCATGCCCA TAGGAAGTGG CCATTTGGGT CTAGGTGTTT TAAGCGGCGT CCAGTCACCA 
TTTTCTTCAT ACGAAAAGCT ATCTTCGATG ATTTCCCCTC CGCCTAATAA CTATTTGGTC 
TCGTGCGATC TACATTCCTC CGTTTCGGGA CGCGTTATAA ATTCATCGCA CTTGCAGCTA 
AGTCACAACG GCAATAAAAA GGAGTCTGGA ACACACGAAC ACACACATAG GCCTGCTGAT 
GTCAATGGTG GGAAGTTTTC CTATACTGGC CACATCTCCC GAGGTGATAG TGTAGATAAT 
GACGTCAACG GTGAAAAGTT CTCTTTCTCT GACCATATCT CCGGTGGGGA TAGCGGAGAT 
GAAGATGCCA ACAGAGAAAA ATTCATATAC TCAGATCACA TCTCCGAAGG AGAAAACGGA 
CCGGACGTCA ATAGCGGAAC GAATTGGCTC CAGATGCACT CTGAGAGGGA AGTGCGGCTC 
CATATGCCCG TGCCGGCTGA GCTGGAAGCG AGGTTTCACA TTTCGTCAGA GCGAAGGACT 
CGACTCAATG TGCCACCTGC TCGTGGTTCT CTAAGCCGAC ATCTTGCTCC AAACGCTCCG 
ATCTGCCCCG CAGATTGGAA AGCGGATGAT TGGAAACACA GCAATGCTGG CGTTGTTAGT 
TTGACCGCAG ATATGCCCGT GGTTGTGTCT CTTACTCCTA CACCGCCTCC GCTAAGAGAC 
GACTCGGTGA GCGGCATTAA ACAAAACGAG CGATCATCAC CAGGGCACAG AGAACAGTAC 
TTCCTGGACA AAAGCCAAGA ACCCAGTTCC GTTGTAGCAG ATCAGTGCAG TCCGGGCATC 
AATGGCACCC CGCAGTCGGT GTGTGTCATT CACCAGCAGT CTCCATCAGC TCTCGGAAAC 
GGGTTTCAAT CAAGCAGCCA GCAGGCCAAG GTTTCCTCGT GCGCATCACC AAAAGGGACA 
GTGTCCTCTG GAGGTGCAGT TTCCAACCGC ATTGCAAATT CCAGTGACAT GGAGGAGATC 
AATACTAAGG ATCTGGCACA GCGCATCTCA GCCGAACTCA AGCGATACAG CATTCCGCAG 
GCTATATTCG CACAACGGGT ACTATGCCGA TCGCAAGGCA CCTTGTCCGA TCTGCTGCGA 
AACCCAAAGC CGTGGTCAAA GCTGAAATCC GGCCGAGAGA CGTTTCGCAG AATGTATAAA 
TGGCTCCAGG AGCCCGAGTT TCAGCGCATG TCTGCTCTGC GAATGGCCGC TGCCCAAATT 
CCTCAGCGAG CTCCTTTGAG CTCTGGAATG TCTCTTGGGT CAGCAACAGG TCCAAGTGGC 
TCCACCGGCA CAATACCGAC GGACTTGGAC CCACATGGCG GCCCTACGAT GATCCAAAAT 
CCTCTGACAA ACGAATCGGA CTCTTCGCCT GCATCAACAC CTGTCACAAG CGTTTTGGTT 
GGGAGCGTCG TTAGCTGTCG TCGAAAGGAA GAGCCCCAGA TCGAACAAAT GCCCCAGCCT 
AAAAAGCCGC GATTGGTCTT CACCGATCTC CAGCGACGAA CATTACAAGC TATTTTCAAA 
GAAACTAAAA GACCTTCGAA GGAGATGCAA GTGACCATCG CGAGACAGCT GGGACTGGAG 
CCTACTACAG TGGGAAATTT TTTTATGAAC GCCAGAAGAA GGTCGATGGA TAAGTGGCGA 
GATGATGACT CCAAAAGCAC TATGCATGTG GCACATAGCC GACAACAACA AGACGAACAG 
CAGAAAGATA ATGCCCACAC TCATAGTCAA TCTCAAATTC AAGATCAAAA TCACTCTCAA 
AATCAAGCTA TCCATAATAG CATGTCGCAA GATCCCTACT CAAATCTTCA CACAACAGCT 
ATGTCGCCTC TTGGAGCTTT TGATGAAGAC GCTGATATGG ACTTGGAACT AGAAAGTCAC 
GACTTTGATT TAGTGGACCC CGATGAACAT GGGGATACCA ATGATCCAAA CGGCGACATG 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524842.2
CDS	948..4193
Translation	MDSLNDIIDHQTFSQELVEDASEFITVGHHSERPSQSSQQPNSGQDLTMSMQDIISCPVKHRTCSASGSGSASGSDSVVMVIDALGQGNRQSAYQIVPQQLQQRNMPLPFGLLERDRQHMQHGREVNTSPVDFVSSDINLDGLTVDADVSQTDHSQETAVKQEQKLLIVQSKSQDQSHRRIRMLVDVSSVNSGLGVHVDDMDEISSDGVGCDDEGVTLSHQHLLEQEEQFGLTSHHPHLQPHTQIIHGLHQRSTHSEMGLDNGHGEVLSVIVHSQDSDKEDCEENDDGDAEGDLENEDDDERDSRSREQLLSHSSYQTLTSVNDRLSSPGFSQTSYATLTPIQPLPPISTMSEKFAYSGHISGGDSGDTDVNGDGAGGGVVEVGEVTNQSSEATGTVSISSGNATSSVCSNNDCSSFSALSMPIGSGHLGLGVLSGVQSPFSSYEKLSSMISPPPNNYLVSCDLHSSVSGRVINSSHLQLSHNGNKKESGTHEHTHRPADVNGGKFSYTGHISRGDSVDNDVNGEKFSFSDHISGGDSGDEDANREKFIYSDHISEGENGPDVNSGTNWLQMHSEREVRLHMPVPAELEARFHISSERRTRLNVPPARGSLSRHLAPNAPICPADWKADDWKHSNAGVVSLTADMPVVVSLTPTPPPLRDDSVSGIKQNERSSPGHREQYFLDKSQEPSSVVADQCSPGINGTPQSVCVIHQQSPSALGNGFQSSSQQAKVSSCASPKGTVSSGGAVSNRIANSSDMEEINTKDLAQRISAELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMYKWLQEPEFQRMSALRMAAAQIPQRAPLSSGMSLGSATGPSGSTGTIPTDLDPHGGPTMIQNPLTNESDSSPASTPVTSVLVGSVVSCRRKEEPQIEQMPQPKKPRLVFTDLQRRTLQAIFKETKRPSKEMQVTIARQLGLEPTTVGNFFMNARRRSMDKWRDDDSKSTMHVAHSRQQQDEQQKDNAHTHSQSQIQDQNHSQNQAIHNSMSQDPYSNLHTTAMSPLGAFDEDADMDLELESHDFDLVDPDEHGDTNDPNGDML

Target ORF information:

RefSeq Version	NM_080103.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster onecut, transcript variant A (onecut), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080103.3

ORF Insert Sequence:

ATGGATTCCT TAAATGATAT AATTGACCAC CAAACATTTA GCCAGGAGTT AGTTGAAGAT 
GCATCGGAGT TCATCACTGT GGGTCATCAT TCGGAGCGCC CTTCGCAGTC CAGTCAGCAG 
CCTAATTCTG GACAGGATCT GACAATGTCT ATGCAAGACA TAATTTCCTG TCCGGTGAAA 
CACCGGACCT GCTCTGCATC TGGGTCGGGA TCGGCATCGG GATCCGATTC CGTTGTCATG 
GTTATAGACG CACTGGGACA AGGTAACAGG CAGTCAGCAT ATCAAATTGT GCCTCAGCAG 
TTGCAGCAAA GAAACATGCC ATTGCCTTTC GGGCTACTTG AACGAGATAG GCAGCATATG 
CAGCATGGGC GTGAGGTCAA TACCAGTCCA GTGGACTTTG TATCATCAGA TATAAATTTG 
GATGGTCTGA CTGTAGACGC AGACGTCTCC CAGACGGATC ACTCTCAAGA AACGGCGGTA 
AAGCAGGAGC AAAAACTACT TATTGTGCAA TCCAAGAGTC AGGATCAAAG CCATAGGCGC 
ATTCGAATGC TTGTAGACGT TTCGAGCGTA AATTCTGGCC TTGGCGTGCA CGTGGATGAT 
ATGGATGAGA TTTCCTCTGA TGGCGTTGGC TGCGACGACG AAGGAGTTAC GCTGAGCCAC 
CAGCATCTTT TGGAGCAAGA GGAACAATTC GGCCTAACAA GCCACCATCC GCATCTCCAA 
CCTCATACAC AGATCATCCA TGGCTTGCAC CAGAGATCAA CACATTCGGA AATGGGACTG 
GACAATGGGC ATGGGGAGGT TCTATCGGTT ATCGTCCATT CCCAGGACAG CGACAAGGAG 
GACTGTGAGG AAAATGATGA TGGGGATGCA GAAGGAGATT TAGAAAATGA AGACGATGAT 
GAACGCGACT CGCGGAGTCG TGAACAGCTG CTTAGTCACA GCTCGTATCA GACGCTGACC 
TCGGTGAATG ATCGCCTATC TTCGCCTGGG TTTAGTCAAA CATCGTATGC TACACTCACC 
CCTATACAAC CACTTCCGCC TATATCAACG ATGTCCGAGA AGTTCGCCTA CTCTGGCCAC 
ATCTCGGGAG GAGACAGTGG AGACACGGAT GTCAATGGAG ATGGTGCAGG TGGAGGAGTC 
GTTGAGGTTG GTGAAGTCAC CAACCAATCC AGCGAGGCTA CAGGGACTGT TTCAATATCT 
AGTGGCAATG CTACATCCTC TGTGTGCTCC AACAACGATT GCAGTTCTTT TTCTGCCCTT 
TCCATGCCCA TAGGAAGTGG CCATTTGGGT CTAGGTGTTT TAAGCGGCGT CCAGTCACCA 
TTTTCTTCAT ACGAAAAGCT ATCTTCGATG ATTTCCCCTC CGCCTAATAA CTATTTGGTC 
TCGTGCGATC TACATTCCTC CGTTTCGGGA CGCGTTATAA ATTCATCGCA CTTGCAGCTA 
AGTCACAACG GCAATAAAAA GGAGTCTGGA ACACACGAAC ACACACATAG GCCTGCTGAT 
GTCAATGGTG GGAAGTTTTC CTATACTGGC CACATCTCCC GAGGTGATAG TGTAGATAAT 
GACGTCAACG GTGAAAAGTT CTCTTTCTCT GACCATATCT CCGGTGGGGA TAGCGGAGAT 
GAAGATGCCA ACAGAGAAAA ATTCATATAC TCAGATCACA TCTCCGAAGG AGAAAACGGA 
CCGGACGTCA ATAGCGGAAC GAATTGGCTC CAGATGCACT CTGAGAGGGA AGTGCGGCTC 
CATATGCCCG TGCCGGCTGA GCTGGAAGCG AGGTTTCACA TTTCGTCAGA GCGAAGGACT 
CGACTCAATG TGCCACCTGC TCGTGGTTCT CTAAGCCGAC ATCTTGCTCC AAACGCTCCG 
ATCTGCCCCG CAGATTGGAA AGCGGATGAT TGGAAACACA GCAATGCTGG CGTTGTTAGT 
TTGACCGCAG ATATGCCCGT GGTTGTGTCT CTTACTCCTA CACCGCCTCC GCTAAGAGAC 
GACTCGGTGA GCGGCATTAA ACAAAACGAG CGATCATCAC CAGGGCACAG AGAACAGTAC 
TTCCTGGACA AAAGCCAAGA ACCCAGTTCC GTTGTAGCAG ATCAGTGCAG TCCGGGCATC 
AATGGCACCC CGCAGTCGGT GTGTGTCATT CACCAGCAGT CTCCATCAGC TCTCGGAAAC 
GGGTTTCAAT CAAGCAGCCA GCAGGCCAAG GTTTCCTCGT GCGCATCACC AAAAGGGACA 
GTGTCCTCTG GAGGTGCAGT TTCCAACCGC ATTGCAAATT CCAGTGACAT GGAGGAGATC 
AATACTAAGG ATCTGGCACA GCGCATCTCA GCCGAACTCA AGCGATACAG CATTCCGCAG 
GCTATATTCG CACAACGGGT ACTATGCCGA TCGCAAGGCA CCTTGTCCGA TCTGCTGCGA 
AACCCAAAGC CGTGGTCAAA GCTGAAATCC GGCCGAGAGA CGTTTCGCAG AATGTATAAA 
TGGCTCCAGG AGCCCGAGTT TCAGCGCATG TCTGCTCTGC GAATGGCCGC TGCCCAAATT 
CCTCAGCGAG CTCCTTTGAG CTCTGGAATG TCTCTTGGGT CAGCAACAGG TCCAAGTGGC 
TCCACCGGCA CAATACCGAC GGACTTGGAC CCACATGGCG GCCCTACGAT GATCCAAAAT 
CCTCTGACAA ACGAATCGGA CTCTTCGCCT GCATCAACAC CTGTCACAAG CGTTTTGGTT 
GGGAGCGTCG TTAGCTGTCG TCGAAAGGAA GAGCCCCAGA TCGAACAAAT GCCCCAGCCT 
AAAAAGCCGC GATTGGTCTT CACCGATCTC CAGCGACGAA CATTACAAGC TATTTTCAAA 
GAAACTAAAA GACCTTCGAA GGAGATGCAA GTGACCATCG CGAGACAGCT GGGACTGGAG 
CCTACTACAG TGGGAAATTT TTTTATGAAC GCCAGAAGAA GGTCGATGGA TAAGTGGCGA 
GATGATGACT CCAAAAGCAC TATGCATGTG GCACATAGCC GACAACAACA AGACGAACAG 
CAGAAAGATA ATGCCCACAC TCATAGTCAA TCTCAAATTC AAGATCAAAA TCACTCTCAA 
AATCAAGCTA TCCATAATAG CATGTCGCAA GATCCCTACT CAAATCTTCA CACAACAGCT 
ATGTCGCCTC TTGGAGCTTT TGATGAAGAC GCTGATATGG ACTTGGAACT AGAAAGTCAC 
GACTTTGATT TAGTGGACCC CGATGAACAT GGGGATACCA ATGATCCAAA CGGCGACATG 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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onecut cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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